ライフサイエンスコーパス: haploid

1 (Puf5), have parallel roles in quiescence in haploids.

2 s, a recently characterized morphotype where haploid 5-mum cells convert to highly polyploid cells of

3 aged the therapeutic factor IX cassette into haploid AAV2/8 1:3 capsids and injected them into FIX kn

4 transduction in the liver was observed with haploid AAV2/8 1:3 than that with AAV8 alone.

5 phenotypic correction were achieved with the haploid AAV2/8 1:3 virus vector when compared to that of

6 be further subdivided into 2 subtypes: near-haploid ALL with 24 to 30 chromosomes and low-hypodiploi

7 ice (Oryza sativa L.), which are effectively haploid, allowing easy haplotype construction and imputa

8 These polyploid titan cells can generate haploid and aneuploid progeny that may result in systemi

9 ugh the duration of interphase is similar in haploid and diploid cells, haploid cells spend longer in

10 le with an alternation between multicellular haploid and diploid generations that facilitated efficie

11 We used haploid and diploid meiotic seed tissues of a single sel

12 phenotype that rapidly drives extinction of haploid and diploid MMR-proficient cells.

13 aryotic sexual life cycles alternate between haploid and diploid stages, the transitions between whic

14 sly diploidize and can be maintained in both haploid and diploid states.

15 Bud sites are selected differently in haploid and diploid yeast cells: haploid cells bud in an

16 We created haploid and heterozygous diploid Tn7 insertional mutagen

17 xamined the performance of screening in both haploid and hypotriploid cell lines, using two alternati

18 sembly projects have been successful in many haploid and inbred species, the assembly of noninbred or

19 Indeed, haploid and repetitive Y chromosomes in species with mal

20 performance to existing methods for diploid, haploid and trio-binned human samples and report superio

21 nt transformation protocol using C. albicans haploids, and an optimized mating strategy to generate h

22 These patterns include haploid- and diploid-phase sex determination, isogamous

23 luated a collection of new de novo long-read haploid assemblies and conclude that although the new as

24 MarginPolish and HELEN polished haploid assemblies to more than 99.9% identity (Phred qu

25 been shown to have similar effects, inducing haploids at high frequencies.

26 e it allows associations to build up between haploid-beneficial alleles and the sex that experiences

27 Using a newly established haploid biofilm model of C. albicans, we found that S. m

28 Cotransfecting dozens of vectors into the haploid blood stages creates complex pools of barcoded m

29 albicans, we found that S. mutans augmented haploid C. albicans accumulation in mixed-species biofil

30 W303 haploids can enter quiescence, but their diploid counter

31 ferent ratios (3:1, 1:1 and 1:3) to assemble haploid capsids and study both their transduction effici

32 The recent development of haploid cell lines has facilitated forward genetic scree

33 at this panel can be used to confirm doubled-haploid cell lines.

34 tures, telomerase activity becomes limiting: haploid cell populations senesce and generate aneuploid

35 e simulations, colonies initiated by an aged haploid cell show declined mating probability at an earl

36 ormed a genome-wide knockout screen in human haploid cells and identified the calcium pump SPCA1.

37 Here, we exploited human haploid cells as a robust comparative screening platform

38 rting approach also enables the isolation of haploid cells at low percentages, as well as the mainten

39 ferently in haploid and diploid yeast cells: haploid cells bud in an axial manner, while diploid cell

40 Because haploid cells can spontaneously become diploid, their en

41 on is due to a proliferative disadvantage of haploid cells compared with diploid cells.

42 membranes (PSMs), the structures that engulf haploid cells during meiosis II (MII).

43 ious environmental conditions, revealed that haploid cells experienced higher rates of silencing loss

44 We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a

45 amental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ c

46 nstable state, so that cultures of mammalian haploid cells rapidly become enriched in diploids.

47 ase is similar in haploid and diploid cells, haploid cells spend longer in mitosis, indicative of pro

48 We performed a gene-trap screen in haploid cells to identify host factors for adenovirus (A

49 sts that non-homologous end-joining, even in haploid cells where homologous chromosomes are not prese

50 establish the physiological role of Ime4 in haploid cells.

51 -activated cell sorting (FACS) enrichment of haploid cells.

52 wide insertional mutagenesis screen in human haploid cells.

53 uring fission yeast karyogamy upon mating of haploid cells.

54 d 7 in TTC7A-knockout (TTC7A-KO) HAP1 (human haploid) cells and reduce the susceptibility to apoptosi

55 On the effectively haploid CHM13 human cell line, HiCanu achieved an NG50 c

56 o eight pseudomolecules corresponding to its haploid chromosome complement, and annotated 27 969 prot

57 locus and loss of chromosome 8p to a single haploid copy recurred at substantial subclonal frequenci

58 nvestigate whether sex-specific selection on haploids could drive the evolution of recombination supp

59 to correctly handle X-chromosome (and other haploid) data from both males and females.

60 ation, we screened the S. pombe nonessential haploid deletion collection and identified 27 gene delet

61 raits in this elite hybrid, a set of doubled haploid (DH) lines derived from ZD958 were evaluated in

62 ap recombinant inbred line (RIL) and doubled haploid (DH) populations from crosses of W7984 by Opata,

63 t with this, a substantial proportion of the haploids die at or shortly after the last mitosis throug

64 Our haploid-diploid eQTL analysis in spruce revealed that co

65 animals, the mitochondrial genome is small, haploid, does not typically recombine, and is subject to

66 s terrestris, using direct sequencing of 299 haploid drone offspring.

67 Using mating-competent C. albicans haploids, each carrying a different gene drive disabling

68 cing organisms starts with the fusion of the haploid egg and sperm gametes to form the genome of a ne

69 During female meiosis, haploid eggs are generated from diploid oocytes.

70 Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian

71 Mammalian haploid embryonic stem cells (haESCs) provide new possib

72 7-week protocol for the derivation of mouse haploid embryonic stem cells (hESCs) from female gametes

73 ogenous leukemia (KBM7 and HAP1), as well as haploid embryonic stem cells derived from several organi

74 Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm de

75 Decreasing nuclear size in post-MBT haploid embryos caused a further delay in cell cycle len

76 and the production of viable parthenogenetic haploid embryos.

77 EPRS-haploid (Eprs(+/-)) mice showed enhanced viremia and inf

78 s well as the maintenance of highly enriched haploid ESC lines throughout passaging.

79 Compared to diploid embryos, haploids exhibited a delay in both zygotic gene expressi

80 y limits imposed on gene expression owing to haploid expression of the X chromosome.

81 tead, the identities of the haploid male and haploid female parents were significant contributors to

82 ecies of cichlid fish in Lake Malawi carry a haploid, female-restricted B chromosome.

83 port that during quiescence, the unicellular haploid fission yeast accumulates mutations as a linear

84 Mice lacking one Pals1 allele (functionally haploid for Pals1) in nephrons developed a fully penetra

85 e functional megaspore, which represents the haploid founder cell of the FG.

86 ich include ants, bees and wasps, develop as haploids from unfertilized eggs.

87 ploid states with some organisms existing as haploids (fungi), diploids (most mammals), and polyploid

88 eproducing organisms use meiosis to generate haploid gametes and faithfully transmit their genome to

89 the specialized cell division that generates haploid gametes and is therefore essential for sexual re

90 mpete for transmission through meiosis, when haploid gametes are created from a diploid parent.

91 In eukaryotic meiosis, generation of haploid gametes depends on the formation of inter-homolo

92 rogram by which a diploid cell gives rise to haploid gametes for sexual reproduction.

93 e and involves the fusion of male and female haploid gametes into a diploid cell.

94 Meiosis generates four genetically distinct haploid gametes over the course of two reductional cell

95 Meiosis produces haploid gametes through a succession of chromosomal even

96 Meiosis, the mechanism of creating haploid gametes, is a complex cellular process observed

97 tion is a crucial step for the production of haploid gametes, which occurs from anterior to posterior

98 two rounds of chromosome segregation to form haploid gametes.

99 omes (homologs) is required for formation of haploid gametes.

100 n meiosis allow diploid organisms to produce haploid gametes: (1) homologous chromosomes (homologs) p

101 s between two multicellular generations, the haploid gametophyte and the diploid sporophyte [1].

102 ive from the chromosome-doubled cells of the haploid gametophyte.

103 the first single-base methylome profiles of haploid gametophytes and diploid sporophytes of a multic

104 d persist as anther somatic cells mature and haploid gametophytes differentiate into pollen.

105 In bryophytes, haploid gametophytes grow via clonal propagation and pro

106 flowering plants relies on the production of haploid gametophytes that consist of germline and suppor

107 highest methylation levels measured in both haploid gametophytes.

108 phytes) depends, in part, on their extensive haploid gene expression.

109 eiotic chromosome stability and in males for haploid gene transcription during postmeiotic sperm diff

110 represents the hallmark between diploid and haploid generations [1].

111 A genome-wide haploid genetic screen identified the transmembrane prot

112 ls (MuCHaChA), which is a novel platform for haploid genetic screening in animals to identify genes e

113 Haploid genetic screening is a powerful tool to reveal f

114 e CRISPR/Cas9-mediated screens together with haploid genetic screens provide a powerful addition to t

115 Using genome-wide haploid genetic screens, here we identify the lipid-modi

116 Although gametes have a haploid genome (n), most mammalian cells have diploid ge

117 Two species with haploid genome assemblies ~150 Mb are among the largest

118 gnant woman was sequenced to a depth of 270x haploid genome coverage.

119 s of detection (LOD(6) values of 1, 2 and 10 Haploid Genome Equivalents).

120 The haploid genome of C. reinhardtii facilitates genetic ana

121 elegans, occurring in ca. 30 400 copies per haploid genome, averaging ca. 1900 copies per telomere,

122 eriments that involve real data, we used two haploid genomes (CHM1 and CHM13) and one human genome (N

123 In an experiment based on 2184 aligned haploid genomes from the 1000 Genomes Project, our algor

124 Doubled haploid production fixes recombinant haploid genomes in inbred lines, shaving years off the b

125 ression in pollen, with actively transcribed haploid genomes.

126 Using WGS sequences of B73xMo17 doubled haploids, genomic locations showing differential repetit

127 recent evolutionary history of a variety of haploid genotype alignments, as it makes no assumptions

128 proteins and RNA, limiting selection on the haploid genotype.

129 ver, significant selection can also occur on haploid genotypes during less conspicuous life cycle sta

130 ly of RNA helicases expressed in meiotic and haploid germ cells which plays an essential role in sper

131 at 8 degrees C gave all homozygous (doubled haploid) gynogenetic progeny with high survival.

132 number that deviates from a multiple of the haploid, has been recognized as a common feature of canc

133 Unlike most MA studies that utilize haploid, homozygous, or self-fertilizing lines, D. pulex

134 ial for AAV serotype 2 (AAV2) infection in a haploid human cell line.

135 hesis, we inactivated six examples in a near-haploid human cell line; five of these mutations increas

136 These lines include near-haploid human cell lines isolated from a patient with ch

137 unbiased genome-wide genetic screen in near-haploid human cells to uncover cellular processes that r

138 Deep mutagenesis of haploid human cells was used to identify host factors re

139 and cell sorting, for the identification of haploid human cells within parthenogenetic ESC lines.

140 ed on the use of a forward genetic screen in haploid human cells, followed by a rigorous single and d

141 generated a clonal cell, HAP1-A12, from near-haploid human cells, in which ATP5G1, ATP5G2, and ATP5G3

142 eens versus gene-trap mutagenesis screens in haploid human cells, which represent the existing 'gold

143 c analysis through reporter-based screens in haploid human cells.

144 We have recently derived haploid human embryonic stem cells (ESCs) by parthenogen

145 Haploid human ES cells exhibited typical pluripotent ste

146 S) cells from several mammalian species, but haploid human ES cells have yet to be reported.

147 Although haploid human ES cells resembled their diploid counterpa

148 We expect that haploid human ES cells will provide novel means for stud

149 isolation of essentially pure populations of haploid human ESCs by this protocol requires basic PSC c

150 ic variation, here we sequence and analyse a haploid human genome (CHM1) using single-molecule, real-

151 Shasta produced a complete haploid human genome assembly in under 6 h on a single c

152 Surprisingly, we found that a haploid human genome is compatible not only with the und

153 e, real-time (SMRT) sequencing data from two haploid human genomes.

154 Haploid human pluripotent stem cells (PSCs) integrate ha

155 We used data generated from a haploid hydatidiform mole genome (CHM1) and a diploid hu

156 fferentially expressed genes between the two haploids in hybrid were associated with either genetic o

157 We found 58% of the haploids in hybrids maintain their parental compartment

158 o circumvent this process to produce doubled haploid individuals, which derive from the chromosome-do

159 lines using intraspecific crosses to in vivo haploid inducer males derived from Stock 6, first report

160 ested in field and sweet corn using a native haploid-inducer line(4) and extended to dicots using an

161 Edited haploid plants lack both the haploid-inducer parental DNA and the editing machinery.

162 o-opted the aberrant reproductive process of haploid induction (HI)(3-6) to induce edits in nascent s

163 such technologies could use in vivo paternal haploid induction (HI), which occurs when double fertili

164 etic complementation, and gene editing, that haploid induction in maize (Zea mays) is triggered by a

165 , and that novel edits in MTL lead to a 6.7% haploid induction rate (the percentage of haploid progen

166 discovery may enable development of in vivo haploid induction systems to accelerate breeding in crop

167 In plants, haploid induction through genome elimination has recentl

168 f pollen-specific genes overexpressed during haploid induction, some of which may mediate the formati

169 ploid and its synthetic diploid produced via haploid induction, to estimate transcriptome size and do

170 the accelerated tumor onset observed in the haploid-insufficient ErbB2 tumors, deletion of both beta

171 We partition the load in haploids into two components.

172 ee major lineages (VNI, VNII, and VNB), some haploid isolates show hybrid ancestry including some tha

173 tenance of human ES cell lines with a normal haploid karyotype.

174 d a loss-of-function genetic screen in human haploid KBM7 cells to discover the mechanism of action o

175 breeding load was low to moderate (0.98-4.66 haploid lethal equivalents) and increased with the time

176 aining reads in the hydatidiform mole (CHM1, haploid-like) genome.

177 urs only in felids, wherein fertilisation of haploid macrogametes by haploid microgametes, results in

178 Instead, the identities of the haploid male and haploid female parents were significant

179 ng males with no Y chromosome genes produced haploid male gametes and sired offspring after assisted

180 Interestingly, the haploid male gametophyte or pollen in Arabidopsis, on th

181 e critical for the specialized maturation of haploid male germ cells called spermiogenesis.

182 Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa,

183 Accordingly, single-cell-sorted haploid mammalian cells maintain the haploid state for p

184 et was used to enrich and sequence a doubled haploid mapping population of hexaploid wheat derived fr

185 between mating success (number of different haploid mates) and fecundity (number of diploid offsprin

186 s in Saccharomyces cerevisiae, in which four haploid meiotic products become encased by prospore memb

187 Our rich catalogue of haploid methylomes across multiple tissues will allow va

188 ein fertilisation of haploid macrogametes by haploid microgametes, results in diploid zygotes, around

189 rocesses essential to progress from a single haploid microgametocyte to the release of eight flagella

190 Haploid microspores undergo polar nuclear migration and

191 butes to diversity and adaptive evolution of haploid (monokaryotic) fungi.

192 Haploid moss gametophytes harbor distinct stem cell type

193 t HO endonuclease is expressed in late G1 in haploid mother cells to initiate mating-type interconver

194 create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting

195 stabilizes haploidy in human HAP1 cells and haploid mouse embryonic stem cells.

196 Only haploid mutator cells with significant lifetime mutation

197 However, because of its repetitive and haploid nature, it is frequently absent from genome asse

198 oom-producing fungi typically contain paired haploid nuclei (n + n), most Armillaria gallica vegetati

199 genome provided by sperm coexist as separate haploid nuclei in the zygote.

200 in dikaryotic fungi (containing two distinct haploid nuclei).

201 the data by excluding heterozygous sites in haploid nuclei, duplicated regions of the genome, and lo

202 ive nuclei are produced by fusions of paired haploid nuclei, they are thought to be diploid (2n).

203 oximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African linea

204 Diploid strains, derived by mating haploids of various genotypes, carried variable numbers

205 induce parthenogenesis and the production of haploid offspring in transgenic sexual pearl millet.

206 rthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and

207 aternal heterozygous SNPs are phased using a haploid PB2 or oocyte as a reference.

208 The occurrence of selection during the haploid phase can have far-reaching consequences for fun

209 ionally display strong defects in the female haploid phase.

210 asic life cycle with alternating diploid and haploid phases.

211 however, is supported by industrial doubled haploid pipelines using intraspecific crosses to in vivo

212 Edited haploid plants lack both the haploid-inducer parental DN

213 le triploid populations, because recombinant haploid pollen produced by diploids allows the apomictic

214 sional fertilization of diploid egg cells by haploid pollen, resulting in triploid apomicts that prod

215 Nearly half of the genome is expressed in haploid pollen, which facilitates genetic analysis, even

216 that can develop without fertilization, but haploid pollen.

217 y form of epistasis, the total response of a haploid population is proportional to the initial total

218 association in the CI 9214/Stirling doubled haploid population mapped Rph1 to the short arm of chrom

219 Here we develop a theoretical model of a haploid population undergoing LGT which includes two new

220 ring per generation for facultatively sexual haploids possessing a biallelic mating-type locus (e.g.,

221 Doubled haploid production fixes recombinant haploid genomes in

222 7% haploid induction rate (the percentage of haploid progeny versus total progeny).

223 Si of haploid PV presents cellular infectivity of a single gen

224 ith its unmutated ancestral strain to create haploid recombinant lines, each carrying an average of 5

225 NHEJ factor DNA Ligase 4 results in enhanced haploid recovery.

226 Compared to traditional haploid reference genomes, graph genomes are an efficien

227 fies the bioinformatic analysis, as only one haploid reference sample is required to establish phase

228 These results indicate that haploid resolution of long-read sequencing data will sig

229 togenesis being most highly expressed in the haploid round spermatid stage.

230 e-feedback (PF) gene circuit integrated into haploid Saccharomyces cerevisiae cells to test if the po

231 imate the mutational load in a population of haploid Saccharomyces cerevisiae that are deficient for

232 present methods for a multiplex CRISPR/Cas9 haploid screen in chimeric axolotls (MuCHaChA), which is

233 Here, we employ a haploid screen to identify sulfated glycosaminoglycans (

234 We performed a genome-wide haploid screen with the EV-D68 prototype Fermon strain t

235 , some of which may mediate the formation of haploid seed.

236 s that zygotic sex-ratios become biased when haploid selected loci become linked to the sex-determini

237 Haploid selected loci can favor recombination suppressio

238 atios, we find that a period of sex-specific haploid selection generally favors recombination suppres

239 Although haploid selection is well established in plants, current

240 eficial alleles and the sex that experiences haploid selection most often (e.g., pollen beneficial al

241 Lastly, I review work on how the haploid selection resulting from pollen competition has

242 In plants, haploid selection should oppose gene loss from Y chromos

243 olution of genes that modify the strength of haploid selection to predict when evolution intensifies

244 ld become enriched for genes that experience haploid selection, as is expected for genes that experie

245 These haploid selective processes are typically sex-specific,

246 of the brown alga Ectocarpus sp. that has a haploid sex determination system (UV system) recovering

247 r understanding the epigenetic regulation of haploid-specific gene expression.

248 lti-omics data from the hybrid mice provided haploid-specific information on the 3D nuclear architect

249 ng event in sexual reproduction, occurs when haploid sperm and egg recognize each other and fuse to f

250 h as the honeybee have been shown to produce haploid sperm through an abortive meiosis.

251 e arrested primary spermatocytes rather than haploid sperm.

252 by which uncommitted germ cells give rise to haploid sperm.

253 iploid mothers to strengthen selection among haploid sperm/pollen, because this reduces the mutation

254 These changes are accompanied by a loss of haploid spermatids due to impeded meiosis.

255 mitotic cell division and the production of haploid spermatids from the tetraploid primary spermatoc

256 anscription levels of the sex chromosomes in haploid spermatids via regulation of postmeiotic sex chr

257 ells including spermatogonia, spermatocytes, haploid spermatids, and spermatozoa, which takes place i

258 spermatogonia to clusters of 64 post-meiotic haploid spermatids, demonstrating that RCs are stable an

259 nal control of mRNA fate in late meiotic and haploid spermatogenic cells.

260 ransformation of stem cells into millions of haploid spermatozoa--is elaborately organized in time an

261 nclude germ-line stem cells, and ending with haploid spermatozoa.

262 l human reproduction is the fusion between a haploid spermatozoon and a metaphase II oocyte.

263 The fusion of the haploid spermatozoon and oocyte is the culminating event

264 aromyces cerevisiae undergo meiosis and form haploid spores, a process collectively referred to as sp

265 s since gametogenesis requires production of haploid spores, which divide and differentiate into spec

266 olates vary greatly in their ability to make haploid spores, with some isolates generating up to 46%

267 them to enter meiosis and differentiate into haploid spores.

268 ordinated with sporulation events to produce haploid spores.

269 the faeces of cats and meiosis gives rise to haploid sporozoites within the oocysts.

270 emical cocktail could maintain haESCs in the haploid state for at least five weeks without fluorescen

271 -sorted haploid mammalian cells maintain the haploid state for prolonged periods, owing to the absenc

272 gression was critical for the maintenance of haploid state.

273 to complete meiosis as demonstrated by their haploid status and the expression of several post-meioti

274 ts, heterothallic species are those in which haploid strains fall into two distinct and stable mating

275 trains is very well studied, but its role in haploid strains has remained unknown.

276 The FG is a few-celled haploid structure that orchestrates division of labor to

277 many crops, while seed-based in vivo doubled haploid systems are rare in nature and difficult to mana

278 redominantly control gene expression for the haploid-to-diploid transition during sexual reproduction

279 nd a processing time nearly one-tenth of the haploid-transformation method.

280 r AAV2), with the highest of these being the haploid vector AAV2/8 3:1.

281 Here we report finding haploid vegetative nuclei in A. gallica at multiple site

282 Additionally, the haploid virus AAV2/8 1:3 was able to escape AAV2 neutral

283 To improve the Nab evasion ability of the haploid virus, we produced the triploid vector AAV2/8/9

284 After muscular injection, all of the haploid viruses induced higher transduction than their p

285 ome constructed in silico by merging the two haploids, we find that approximately 59% of the heterozy

286 Haploids were obtained after cenh3 L130F-complemented ce

287 observation that inducer lines do not induce haploids when crossed to themselves.

288 ta=4N e mu for diploids or Theta=2N e mu for haploids (where N e is the effective population size and

289 Unlike land plants, it is a haploid with very few gene duplicates, making it ideal f

290 Interestingly, haploids with increased N/C volume ratios exhibited an i

291 romosomes; females are diploid and males are haploid, with sex usually determined by heterozygosity a

292 (several versions of the Moran model and the haploid Wright-Fisher model) to examine fixation probabi

293 ns of the Moran process, as well as for the (haploid) Wright Fisher model are presented.

294 Haploid yeast cells secrete mating pheromones that are s

295 Exposure of haploid yeast cells, carrying mating type "a," to "alpha

296 esence of m(6)A-modified FAA1 transcripts in haploid yeast cells.

297 d for strain validation by WGS, we sequenced haploid yeast segregants derived from a popular commerci

298 We applied MeSMLR-seq to haploid yeast, where single DNA molecules represent sing

299 for cell survival and mating-type switch in haploid yeast.

300 of a reference genome assembly for a double haploid YY male garden asparagus (Asparagus officinalis