ライフサイエンスコーパス: hemicholinium 3

1 competitive, high-affinity uptake inhibitor, hemicholinium-3.

2 haracterized inhibitor of choline transport, hemicholinium-3.

3 250 microM) and the choline kinase inhibitor hemicholinium-3 (2 mM) enhanced and inhibited, respectiv

4 able ACh using inhibitors of choline uptake (hemicholinium-3; 20-50 microM) or vesicular ACh transpor

5 lted in a persistent 30-40% reduction of [3H]hemicholinium-3 ([3H]HC-3) binding in the hippocampus th

6 lls, whereas only FCH was inhibited (90%) by hemicholinium-3, a specific inhibitor of choline transpo

7 the reductions of ACh release, vesamicol and hemicholinium-3 also decreased H82 cell proliferation.

8 ptake and vesicular acetylcholine release by hemicholinium-3 also enhanced stretch-evoked responses.

9 Vesamicol, an inhibitor of VAChT, and hemicholinium-3, an inhibitor of choline transport, both

10 n preparations stimulated in the presence of hemicholinium-3, an inhibitor of choline uptake, or in N

11 ited by the structurally similar derivative, hemicholinium-3 and acetylcholine, but not by substrates

12 gated due to inhibition of ACh production by hemicholinium-3 and restored by exogenously added carbac

13 Intracerebroventricular injection of hemicholinium-3 at doses known to deplete the endogenous

14 ne acetyltransferase activity (ChAT) and [3H]hemicholinium-3 binding (HC-3) in the hippocampus, midbr

15 ne acetyltransferase activity (ChAT) and [3H]hemicholinium-3 binding (HC-3) were monitored; ChAT is a

16 sferase, high-affinity choline transport and hemicholinium-3 binding to the choline carrier were redu

17 Hemicholinium-3 binding was reduced in ventral and dorsa

18 activity of the choline transporter and the hemicholinium-3 binding were decreased in all spinal seg

19 nergic markers choline acetyltransferase and hemicholinium-3 binding, a marker for the high-affinity

20 ted with a reduction of ChAT activity (30%), hemicholinium-3 (HC-3) binding (40%), and AChE-positive

21 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline

22 Hemicholinium-3 (HC-3), an inhibitor of choline kinase,

23 CHT1 is a Na+-dependent, hemicholinium-3 (HC-3)-sensitive choline transporter.

24 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline

25 overy was blocked by (-)-vesamicol (VES), by hemicholinium-3 (HC3) and by nicotinic cholinergic agoni

26 take studies were performed with and without hemicholinium-3 (HC3), a selective inhibitor of high aff

27 incipal inhibitors were (-)-vesamicol (VES), hemicholinium-3 (HC3), and NH4+.

28 The choline kinase inhibitor hemicholinium-3 inhibits approximately 60% of choline-me

29 nhibited, when the choline uptake inhibitor, hemicholinium-3, is present or when extracellular Na+ (r

30 s well documented that the sodium dependent, hemicholinium-3 sensitive, high affinity choline co-tran

31 y of choline, acquired by a plasma membrane, hemicholinium-3-sensitive (HC-3) choline transporter (CH

32 uptake of choline via the sodium-dependent, hemicholinium-3-sensitive choline transporter (CHT) is b

33 and release is thought to be sustained by a hemicholinium-3-sensitive choline transporter (CHT).

34 Hemicholinium-3-sensitive choline uptake and subsequent

35 dividuals revealed significant reductions in hemicholinium-3-sensitive choline uptake, a finding cons

36 f OCT1 and OCT2 in Xenopus oocytes increased hemicholinium-3-sensitive choline uptake.

37 cultures, we demonstrate that CHO-1 mediates hemicholinium-3-sensitive, high-affinity choline uptake

38 high-affinity choline transporter antagonist hemicholinium-3 similarly increased muscle spindle affer