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1 ich expressed one of the ATPases tagged with hexahistidine.
2 aining the photo-tag: 4-benzoylbenzyl-glycyl-hexahistidine.
3 uitin (Ub) monomers N-terminally tagged with hexahistidine.
4 toplasmic proteins expressing the ubiquitous hexahistidine affinity handle can be specifically attach
7 m-affinity purification (TAP) approach using hexahistidine and BirA-specific biotinylation tags for i
8 ith a hexahistidine tag at the N terminus or hexahistidine and c-myc tags at the C terminus was able
10 using a strategy based on two affinity tags (hexahistidine and FLAG) engineered into the N terminus o
11 ing analysis was facilitated by insertion of hexahistidine and short biotinylation sequences on the d
12 eceptors extended on the amino terminus with hexahistidine and the FLAG epitope, DYKDDDDK (H/F-A(2B))
15 (YARKARRQARR) at the amino-terminal end and hexahistidine at the carboxy-terminal end to generate CP
16 ) expression of a f310 fusion construct with hexahistidine at the N-terminus of the coding region all
18 ce (SPR) sensor surface of recombinant human hexahistidine cyclophilin A (His-CypA) is described.
27 hanced the endocytosis of AcGFP by 150-fold (hexahistidine fusion protein) or 600-fold (decahistidine
28 onsible for this interaction, we constructed hexahistidine fusion proteins from different regions of
29 a and IHFbeta were expressed and purified as hexahistidine fusion proteins, and using electrophoretic
31 rio parahaemolyticus, tagged with C-terminal hexahistidine, has been purified to apparent homogeneity
34 Zn(2+) ions binds tightly but reversibly to hexahistidine (His(6)) motifs on surface-exposed protein
35 onal screen utilizes the frequently employed hexahistidine (His(6)) tag as a reporter, and measures i
37 JVZ-007 was initially produced with a c-myc-hexahistidine (his) tag allowing purification and detect
38 over-expressed and purified as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc
41 (H)H fragment was equipped with a C-terminal hexahistidine (His6) tether to facilitate the assembly o
42 y fusing the protein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubiquitin
45 ts to the green fluorescent protein (GFP) or hexahistidine in sporulating B. subtilis or in Escherich
46 ell line has been produced that incorporates hexahistidine-labeled p43 into the multisynthetase compl
47 omitans DspB protein engineered to contain a hexahistidine metal-binding site at its C terminus in Es
48 Microbial growth studies indicate that the hexahistidine motif is important for preventing microbia
49 rt that CP binds Zn(II) at this site using a hexahistidine motif, completed by His103 and His105 of t
51 the green fluorescent protein AcGFP fused to hexahistidine or decahistidine peptides (3 microM) and N
54 istidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, whi
55 nding protein and a polypeptide containing a hexahistidine sequence as the N- and C-exteins, respecti
57 binantly engineered to express an N-terminal hexahistidine sequence was expressed from a eukaryotic p
58 des an influenza hemagglutinin epitope and a hexahistidine sequence, permitting sensitive detection a
59 establish that coordination of Ni(II) at the hexahistidine site is thermodynamically preferred over Z
60 II) affinity at a biologically unprecedented hexahistidine site that forms at the S100A8/S100A9 inter
62 h serine and the C terminus was fused with a hexahistidine tag and (ii) this protein was allowed to f
63 hypothesis, TetA(L) has been purified with a hexahistidine tag at its C terminus and reconstituted in
65 dition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of e
69 e expressed proHP14 with a carboxyl-terminal hexahistidine tag in a baculovirus/insect cell system an
70 the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed due to the
72 hat recombinant, purified ICP47 containing a hexahistidine tag inhibits peptide import into microsome
73 lished using a plasmid carrying abgAB with a hexahistidine tag on the carboxyl terminus of AbgB and s
74 hip, or noncovalently bound via a C-terminal hexahistidine tag on the protein to Ni(2+)nitrilotriacet
75 e factor ectodomain via the engineering of a hexahistidine tag onto its C-terminus and its use in com
76 gs show that the catalytic properties of the hexahistidine tag should be taken into consideration whe
79 combinant PRG B protein (r-PRG B) fused to a hexahistidine tag was purified and analyzed for structur
81 s a recombinant protein (r-NTP) containing a hexahistidine tag, and refolded to the native structure
82 e human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacter
83 mutants with C-terminal deletions fused to a hexahistidine tag, we determined that the processing sit
92 us solution by electrostatic coupling of the hexahistidine tagged OPH (His6-OPH) and poly(ethylene gl
93 bacterial expression system for a C-terminal hexahistidine tagged version of the native enzyme, which
96 garose column was dependent on expression of hexahistidine-tagged alpha(i) and resulted in purificati
97 eterotrimeric G proteins were expressed with hexahistidine-tagged alpha(i) in insect cells, a heterot
98 to the medium can block lysis by a T protein hexahistidine-tagged at its C-terminus, suggesting that
101 displacement from inside-out vesicles by the hexahistidine-tagged cytoplasmic domain of band 3, cdb3-
103 an plasminogen was expressed in E. coli as a hexahistidine-tagged fusion protein and chromatographica
105 ytic site, have been expressed as individual hexahistidine-tagged fusion proteins in the baculovirus
106 ds displaying chelated nickel bound purified hexahistidine-tagged G protein heterotrimers and, subseq
108 r could be assembled with partially purified hexahistidine-tagged gamma(2) in vitro to form a functio
109 derivatized to carry chelated nickel to bind hexahistidine-tagged green fluorescent protein (GFP) and
112 in, we purified and characterized C-terminal hexahistidine-tagged human CLN2p/tripeptidyl-peptidase I
113 ted the suitability of applying C-terminally hexahistidine-tagged interleukin-12 (IL-12) alpha- and b
114 ic acid (NTA)-coordinated metals avidly bind hexahistidine-tagged macromolecules, including peptides
119 es were isolated from PS120 cells expressing hexahistidine-tagged NHERF-1 and NHE3 using nickel-NTA-a
122 ophic yeast Pichia pastoris and purified the hexahistidine-tagged protein by immobilized metal affini
124 l describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorpt
125 ilotriacetic acid lipid analog that can bind hexahistidine-tagged proteins, BAK oligomers were formed
126 -five site-specific mutants were produced as hexahistidine-tagged proteins, purified, and examined fo
129 cetate ligand was immobilized for binding of hexahistidine-tagged red-fluorescing TagRFP, while an ap
131 y plates were used for purifying recombinant hexahistidine-tagged Triticum aestivum (wheat) chlorophy
134 (called aasS) encodes a protein of 60 kDa, a hexahistidine-tagged version of which was readily expres
137 ides a simple approach to exposing concealed hexahistidine tags while retaining native noncovalent pr
139 e was fused to the amino terminus, and (3) a hexahistidine tail was added to the carboxyl terminus.
141 inding protein (PBP) tagged by an N-terminal hexahistidine tail which was immobilized onto Co(2+)-tet