ライフサイエンスコーパス: hnRNP A1

1 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1).

2 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1).

3 nctions by binding of the splicing repressor hnRNP A1.

4 tif that mediates bidirectional transport of hnRNP A1.

5 affinity and specificity similar to those of hnRNP A1.

6 les as the nucleocytoplasmic shuttle protein hnRNP A1.

7 ong similarity to the RNA-binding domains of hnRNP A1.

8 and the Gly-rich region of the C terminus of hnRNP A1.

9 Importantly, tax MAbs reacted with hnRNP A1.

10 ponents, including the heteronuclear protein hnRNP A1.

11 n and of the general pre-RNA-binding protein hnRNP A1.

12 gle-stranded overhangs of human telomeres by hnRNP A1.

13 of either RRM impairs splicing repression by hnRNP A1.

14 s conform to the consensus binding motifs of hnRNP A1.

15 ing site for the pre-mRNA processing protein hnRNP A1.

16 nergistic manner with the RNA-binding factor hnRNP A1.

17 mediated by the cytoplasmic accumulation of hnRNP A1.

18 ent cells that express exogenous cytoplasmic hnRNP A1.

19 methylation of specific arginine residues on hnRNP-A1.

20 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), a protein with multiple roles in mRNA metabol

21 hTRN1 interacts with the M9 region of hnRNP A1, a 38-amino-acid domain rich in Gly, Ser, and A

22 We recently found that hnRNP A1, a protein implicated in many aspects of RNA pr

23 NP complexes from HeLa cells associated with hnRNP A1, a shuttling hnRNP protein.

24 icing of HPV18 E6E7 pre-mRNAs via binding to hnRNP A1, a well-characterized, abundantly and ubiquitou

25 A screen identified hnRNP A1 (A1) and RPS25 as IRES-binding trans-acting fac

26 creen to identify novel ITAFs and identified hnRNP A1 (A1) as a mediator of the IL-6 effect.

27 The RNA-binding protein hnRNP A1 (A1) was also critical to IL-6-stimulated trans

28 Telomere bound hnRNPs include hnRNP A1, A2-B1, D and E and telomerase bound hnRNPs inc

29 Most importantly, the expression of hnRNP A1/A2 and PTB/nPTB is significantly altered in pat

30 /CG9983, the fruit fly ortholog of the human hnRNP A1/A2 factors.

31 protein overexpression, and found two tandem hnRNP A1/A2 motifs within the ISS that are responsible f

32 essing cell extracts with an antibody to the hnRNP A1/A2 proteins, which are splicing and nuclear shu

33 ding proteins, such as hnRNP L, PTB/nPTB and hnRNP A1/A2.

34 inhibitory effect of ISTL1 is independent of hnRNP A1/A2B1 and PTB1 previously implicated in SMN2 exo

35 Overexpression of hnRNP A1 accelerated the kinetics of viral RNA synthesis

36 odynamic coupling between the tandem RRMs of hnRNP A1 accounts for its allosteric regulatory function

37 In the present study, we demonstrate that hnRNP A1 also participates in the transcription and repl

38 Importantly, knockdown of hnRNP A1 also renders quiescent Akt-containing cells sen

39 Mutants in the 5' INS that prevent hnRNP A1 and 50 kDa protein binding are inactive in the

40 nstrate that the related splicing repressors hnRNP A1 and A2, as well as the polypyrimidine-tract-bin

41 We used RNA sequencing (RNA-seq) to confirm hnRNP A1 and A2/B1 motif-dependent roles genome-wide, pr

42 o 40) of p17 that is critical for binding to hnRNP A1 and for nucleocytoplasmic shuttling of p17.

43 a marked increase in the protein levels FUS, hnRNP A1 and hnRNP E2, three RNA-binding proteins involv

44 Although hnRNP A1 and HuR can both be immunoprecipitated from rib

45 voring recruitment of the splicing repressor hnRNP A1 and interfering with that of U2AF65 at the 3' s

46 This study provides novel insights into how hnRNP A1 and lamin A/C modulate nucleocytoplasmic shuttl

47 Here we report that hnRNP A1 and lamin A/C serve as carrier and mediator pro

48 this study provide mechanistic insights into hnRNP A1 and lamin A/C-modulated nucleocytoplasmic shutt

49 sults reveal general rules of specificity of hnRNP A1 and provide a quantitative framework for unders

50 Thus, hnRNP A1 and PTB use different mechanisms to repress c-s

51 Furthermore, hnRNP A1 and PTB, both of which also bind to the complem

52 progression and a coordinated regulation by hnRNP A1 and Sam68 as modifiers of hypoxia-induced skipp

53 a treatment in cell culture led to increased hnRNP A1 and Sam68 levels.

54 ing of SMN exon 7 and was found to bind both hnRNP A1 and Sam68.

55 The activities of hnRNP A1 and SF2/ASF on N1 splicing were examined by add

56 n-proximal 5' splice sites, and tethering of hnRNP A1 and SF2/ASF proteins between competing splice s

57 Mutations in hnRNP A1 and SF2/ASF show that the opposite effects of t

58 differentiation, HPV16 infection upregulates hnRNP A1 and SF2/ASF, both key factors in alternative sp

59 ionship between the potential for binding of hnRNP A1 and U1 snRNA and the effect of hnRNP L on splic

60 heterogeneous nuclear ribonucleoprotein-A1 (hnRNP-A1), and a previously uncharacterized protein, FLJ

61 Further, we found hnRNP L, hnRNP A1, and hnRNP A2B1 bind UNC13A RNA and repress cry

62 s nuclear ribonucleoprotein family (hnRNP L, hnRNP A1, and hnRNP M) were identified.

63 tax MAbs inhibited antibody binding to tax, hnRNP A1, and neurons.

64 ugh interaction with a host splicing factor, hnRNP A1, and regulates E6 and E7 expression of the earl

65 stitutive transcriptional repressor protein, hnRNP A1, and the decreased binding activity of hnRNP A1

66 d the presence of two mRNA-binding proteins, hnRNP A1, and the elav-like protein, HuR, both of which

67 HnRNP A1 antagonizes ASF/SF2 by promoting the distal cho

68 al gray matter of the spinal cord where anti-hnRNP A1 antibodies localized.

69 Anti-hnRNP A1 antibodies were found to surround neuronal cell

70 Additionally, anti-hnRNP A1 antibodies were found within neuronal cell bodi

71 s (EAE), we show here that injection of anti-hnRNP A1 antibodies, in contrast to control antibodies,

72 y shown to undergo neurodegeneration in anti-hnRNP A1 antibody injected EAE mice.

73 osition of betaARB (HuR alone versus HuR and hnRNP A1) appears to be dependent on the mRNA probe used

74 neous nuclear ribonucleoprotein (hnRNP) L or hnRNP A1 are Akt substrates during Treg induction and ha

75 The two RRMs of hnRNP A1 are closely related but have distinct functions

76 These data point to hnRNP A1 as a critical regulator of c-myc translation an

77 Although the importance of hnRNP A1 as a regulator of nuclear pre-mRNA and mRNA pro

78 Here we identify hnRNP A1 as an IRES trans-acting factor that regulates c

79 dentified heterogenous ribonucleoprotein A1 (hnRNP A1) as a G-quadruplex-unwinding helicase, which un

80 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) as a possible mechanism of neurodegeneration i

81 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) as the autoantigen.

82 d heterogenous nuclear ribonucleoprotein A1 (hnRNP-A1) as a pharmacodynamic biomarker of type I PRMT

83 eterogeneous nuclear ribonuclear protein-A1 (hnRNP-A1) as the autoantigen.

84 AKT phosphorylation of hnRNP A1 at serine 199 has been demonstrated to inhibit

85 ely, our findings uncover an MYC-driven DKC1-hnRNP A1 axis that links IRES-dependent translation and

86 NA, they facilitate cooperative spreading of hnRNP A1 between the two sites.

87 thus show that two cellular hnRNPs, PTB and hnRNP A1, bind to the transcription-regulatory sequences

88 We report here that hnRNP A1 binding and splicing repression can occur on an

89 restore exon 7 inclusion in vivo and prevent hnRNP A1 binding in vitro.

90 ng and in vitro evolution identify consensus hnRNP A1 binding motifs; however, such data do not revea

91 negative strand of the 3'-UTR also inhibited hnRNP A1 binding on the positive strand, indicating a po

92 ructed MHV mutants with a very high-affinity hnRNP A1 binding site inserted in place of, or adjacent

93 When the high affinity hnRNP A1 binding site was inserted into the beta-globin

94 This inserted hnRNP A1 binding site was not able to functionally repla

95 se change does indeed create a high-affinity hnRNP A1 binding site, and base substitutions that disru

96 In contrast, the mutant hnRNP A1 binding site, or binding sites for hnRNP C and

97 , potentially creates a second SMN2-specific hnRNP A1 binding site.

98 We show that the SC35 and the hnRNP A1 binding sites overlap within the juxtaposed ESE

99 -related exon-skipping mutations that create hnRNP A1 binding sites, but show that splicing can be re

100 Initial hnRNP A1 binding to an exonic splicing silencer at the 3

101 solution as a dimer and supports a model for hnRNP A1 binding to nucleic acids in arrays that may mak

102 We propose that hnRNP A1 binding to the ESS inhibits splicing of the ups

103 arose by cytoplasmic hnRNP A1, while nuclear hnRNP A1 binding was unaffected.

104 solution, which could potentially influence hnRNP A1 binding.

105 A high-affinity hnRNP A1-binding sequence can substitute functionally fo

106 ulatory elements as follows: a high affinity hnRNP A1-binding site (WA1), a sequence involved in Rev-

107 ective-interfering RNAs containing a mutated hnRNP A1-binding site have reduced RNA transcription and

108 Introduction of point mutations into the hnRNP A1-binding site or knockdown of hnRNP A1 expressio

109 A strong hnRNP A1-binding site was located 90 to 170 nucleotides

110 Mutation of hnRNP A1-binding sites prevented hypoxia-induced skippin

111 hnRNP A1 binds both IRESs in vitro and in intact cells a

112 hnRNP A1 binds to RNA in a cooperative manner.

113 Serine 199-phosphorylated hnRNP A1 binds to the IRESs normally but is unable to su

114 We show that hnRNP A1 binds to the loop of pri-miR-18a and induces a

115 e presence of excess protein, we reveal that hnRNP A1 binds with selectivity to SL3 through mechanism

116 Recombinant hnRNP A1 bound to these two RNA regions in vitro in a se

117 hroleukemia cell line, CB3, does not express hnRNP A1 but still supports MHV replication, suggesting

118 re, we demonstrate that in basal conditions, hnRNP A1, but not hnRNP A1B, represses interferon stimul

119 ing led us to test more directly the role of hnRNP A1 by analysis of MHV replication and RNA synthesi

120 progenitor 32Dcl3 cells, BCR/ABL stabilizes hnRNP A1 by preventing its ubiquitin/proteasome-dependen

121 We further show that hnRNP A1 can also spread in a 5'-to-3' direction, althou

122 hnRNP A1 can be proteolyzed to unwinding protein (UP1),

123 n addition, we show by NMR that both RRMs of hnRNP A1 can bind simultaneously to a single bipartite m

124 Moreover, hnRNP A1 can effectively unwind an RNA hairpin upon bind

125 ss-linking experiments show that SF2/ASF and hnRNP A1 compete to bind pre-mRNA, and we conclude that

126 To confirm that the hnRNP A1 complex is responsible for INS activity, a synt

127 to present a detailed description of the 7SK-hnRNP A1 complex.

128 e of UP1, the amino-terminal domain of human hnRNP A1 containing two RNA-recognition motifs (RRMs), b

129 A1 and depends on the formation of a FUS-Jun-hnRNP A1-containing complex and on lack of PKCbetaII pho

130 Nuclear and cytoplasmic hnRNP A1 could be distinguished by the differential sens

131 Antibodies to hnRNP-A1 cross-reacted with HTLV-1-tax, the immune respo

132 romoting distal splicing was also seen in an hnRNP A1-deficient MEL cell line.

133 tion on SMN2 exon 7 splicing is specific, as hnRNP A1 depletion has little or no effect on other inef

134 We demonstrate that the striking effect of hnRNP A1 depletion on SMN2 exon 7 splicing is specific,

135 n be restored only modestly or not at all by hnRNP A1 depletion.

136 By comparison, hnRNP A1 directly binds to the A3 consensus cleavage sit

137 With longer flap substrates the hnRNP A1 effect is more modest and is suppressed by the

138 Thus, cytoplasmic and nuclear hnRNP A1 exhibit different RNA binding profiles, perhaps

139 to the hnRNP A1-binding site or knockdown of hnRNP A1 expression promoted 233^416 splicing and reduce

140 The increase in AUBP activity of cytoplasmic hnRNP A1 following RNA polymerase II inhibition correlat

141 We determined the affinity of hnRNP A1 for all possible sequence variants (n = 16,384)

142 organization of the two RRMs is essential to hnRNP A1 function.

143 The cellular absence of hnRNP A1 had no detectable effect on the production of i

144 Whereas nuclear hnRNP A1 has been shown to bind intronic sequences and m

145 heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) has been implicated in telomere protection and

146 Drosophila Hrp38, a homolog of human hnRNP A1, has been shown to regulate splicing, but its f

147 Notably, hnRNP A1, hnRNP A2, and hnRNP B1 bound to many dispersed

148 geneous nuclear ribonucleoproteins (hnRNPs), hnRNP A1, hnRNP A2/B1, and hnRNP Q, bind to the dengue v

149 The sumoylation of hnRNP A1, hnRNP F, and hnRNP K were confirmed in vivo by

150 This sequence was also specifically bound by hnRNP A1, hnRNP H, ASF/SF2 and SRp40, but not by 9G8.

151 s binding to this element were identified as hnRNP A1, hnRNP H, hnRNP F, and SF2/ASF by site-specific

152 Here, we identify the mRNAs bound to the hnRNP-A1, hnRNP-E2, hnRNP-K, and La/SSB RBPs in BCR/ABLt

153 vary gland cells with fluorescent hrp36, the hnRNP A1 homolog, and the nuclear envelope by fluorescen

154 Drosophila hnRNP A1, Hrp38, is required for germ line stem cell mai

155 a suggest that seemingly disparate roles for hnRNP A1 in alternative splice site selection, RNA proce

156 tional studies failed to indicate a role for hnRNP A1 in ARE-dependent mRNA turnover.

157 quired for the subcellular redistribution of hnRNP A1 in cells subjected to OSM.

158 esting that alternative proteins can replace hnRNP A1 in cellular functions and viral infection.

159 RNA foci with SRSF2, hnRNP H1/F, ALYREF and hnRNP A1 in cerebellar granule cells and with SRSF2, hnR

160 , stress-induced cytoplasmic accumulation of hnRNP A1 in MDA-MB-468 cells dynamically alters the CEAC

161 These results support a role for hnRNP A1 in mediating rapamycin-induced alterations of c

162 r findings demonstrate that the functions of hnRNP A1 in MHV RNA synthesis can be replaced by other c

163 involved in the cytoplasmic accumulation of hnRNP A1 in NIH 3T3 cells subjected to OSM.

164 This effect of hnRNP A1 in promoting distal splicing was also seen in a

165 hnRNPs have previously been shown to replace hnRNP A1 in regulating RNA splicing.

166 ha phosphorylation-dependent accumulation of hnRNP A1 in SGs.

167 gardless of the presence of P-TEFb, HEXIM or hnRNP A1 in the complex.

168 ay, which in turn results in accumulation of hnRNP A1 in the cytoplasm.

169 The exact role of HuR and hnRNP A1 in the regulation of beta-AR mRNA stability rem

170 cular mimicry between an infecting agent and hnRNP-A1 in autoimmune disease of the CNS.

171 The role of hnRNP-A1 in telomere protection also involves DNA-depend

172 Two of the human MARs bind hnRNP-A1 in vitro directly within a 35-bp sequence that

173 heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) in vivo during transcriptional up-regulation o

174 Expressing GzmA-resistant hnRNP A1 inhibited GzmA-mediated cell death and rescued

175 ral CB3 cellular proteins similar in size to hnRNP A1 interacted with the MHV RNA.

176 t comprise the NES can modulate both p17 and hnRNP A1 interaction and nucleocytoplasmic shuttling of

177 y attenuates viral replication by abrogating hnRNP A1 interactions.

178 plicing towards the downstream site, whereas hnRNP A1 interferes with U1 snRNP binding such that 5'SS

179 These data suggest that hnRNP A1 is a cellular factor that regulates the RNA-dep

180 hnRNP A1 is a nucleocytoplasmic shuttling heterogeneous

181 Human hnRNP A1 is a versatile single-stranded nucleic acid-bin

182 We conclude that hnRNP A1 is able to direct unspliced globin pre-mRNA int

183 d rescued pre-mRNA splicing, suggesting that hnRNP A1 is an important GzmA substrate.

184 sequences and modulate splicing, cytoplasmic hnRNP A1 is associated with poly(A)+ RNA, indicating dif

185 Previous studies indicated that cytoplasmic hnRNP A1 is capable of high-affinity binding of reiterat

186 une response between HTLV-I tax and neuronal hnRNP A1 is contained within the human immunodominant ep

187 in testis out of six mouse tissues, whereas hnRNP A1 is down-regulated during germ cell development.

188 that the recruitment of cellular proteins by hnRNP A1 is important for MHV RNA synthesis.

189 sults suggest that the shuttling activity of hnRNP A1 is important for the nucleocytoplasmic traffick

190 We showed that hnRNP A1 is methylated by PRMT5 on two residues, R218 an

191 The stimulation by hnRNP A1 is most dramatic using DNA substrates with shor

192 These results strongly suggest that hnRNP A1 is not a required host factor for MHV discontin

193 The region consisting of residues 1-195 of hnRNP A1 is referred to as UP1.

194 Here we report that hnRNP-A1 is phosphorylated by DNA-PKcs during the G2 and

195 Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multipurpose RNA-binding protein (RBP) in

196 Heterogeneous ribonucleoprotein A1 (hnRNP A1) is a prototype for the family of eukaryotic RN

197 Heterogeneous ribonucleoprotein A1 (hnRNP A1) is an abundant nuclear protein that participat

198 Heterogeneous nuclear ribonucleoprotein (hnRNP A1) is involved in pre-mRNA splicing in the nucleu

199 The heterogeneous nuclear ribonucleoprotein, hnRNP A1, is an IRES transacting factor (ITAF) that regu

200 NP A1, and the decreased binding activity of hnRNP A1 leads to the overexpression of gamma chain in H

201 We show here that hnRNP A1 levels are increased in myeloid progenitor cell

202 trongly correlated with elevated Ser(P)(199)-hnRNP A1 levels in a panel of 22 glioblastomas.

203 documented changes in the abundance of these hnRNP A1-like proteins during embryogenesis.

204 Furthermore, as hnRNP A1 likely participates in mRNA export, it raises t

205 and pathophysiological conditions that alter hnRNP A1 localization, are not known.

206 Although the Tap NLS and the hnRNP A1 M9 element are shown to compete for transportin

207 ndicated that direct interaction of p17 with hnRNP A1 maps within the amino terminus (amino acids [aa

208 ally constitutively spliced, suggesting that hnRNP A1 may be a general inhibitor of proximal splicing

209 it is the major determinant of Tra2beta- and hnRNP A1-mediated regulation.

210 We propose that interactions between hnRNP A1 molecules bound to the exonic and intronic site

211 The hnRNP A1 mutant caused a global inhibition of viral mRNA

212 Similar to the wild-type hnRNP A1, the hnRNP A1 mutant complexed with an MHV polymerase gene pr

213 sion in the cytoplasm of a dominant-negative hnRNP A1 mutant that lacks the nuclear transport domain

214 ell-signaling pathways regulates the rate of hnRNP A1 nuclear import.

215 Neurons displayed increased levels of hnRNP A1 nucleocytoplasmic mislocalization and stress gr

216 To assess the potential role of hnRNP A1 nucleocytoplasmic shuttling activity in normal

217 IRES activity by inducing phosphorylation of hnRNP A1 on serine 199.

218 The negative activity of hnRNP A1 on splicing was compared with that of PTB, a pr

219 Depletion of hnRNP A1 or L by RNAi in HEK-293 cells promoted exon 7 i

220 Knockdown of hnRNP A1 or lamin A/C led to inhibition of nucleocytopla

221 Reducing expression levels of hnRNP A1 or overexpressing a dominant negative version o

222 Consequently, in cells lacking hnRNP-A1 or DNA-PKcs-dependent hnRNP-A1 phosphorylation,

223 al gel and proteolysis studies, we establish hnRNP A1 (or structurally related proteins that are post

224 lation in vivo and correlates with increased hnRNP A1 phosphorylation.

225 our results indicate that DNA-PKcs-dependent hnRNP-A1 phosphorylation is critical for capping of the

226 he G2 and M phases and that DNA-PK-dependent hnRNP-A1 phosphorylation promotes the RPA-to-POT1 switch

227 cells lacking hnRNP-A1 or DNA-PKcs-dependent hnRNP-A1 phosphorylation, impairment of the RPA-to-POT1

228 ng was antagonized by cotransfection with an hnRNP A1 plasmid.

229 s suggest that autoimmunity to RBPs, such as hnRNP A1, play a role in neurodegeneration in EAE with i

230 ecent evidence has further demonstrated that hnRNP-A1 plays a crucial role in maintaining newly repli

231 Previous studies suggested that hnRNP A1 preferentially binds (under nonequilibrium cond

232 We additionally demonstrate that cytoplasmic hnRNP A1 preferentially binds ARE relative to pre-mRNAs

233 RNA interference to knock down hnRNP A1 prevented an IL-6 increase in myc protein expre

234 As hnRNP A1 propagates toward the 5' end of the exon, it an

235 he Kap beta2-mediated nuclear import of host hnRNP A1 protein and, in this way, favors virion formati

236 novel 38 amino acid transport signal in the hnRNP A1 protein, termed M9, which confers bidirectional

237 These studies suggest that hnRNP A1-PTB interactions provide a molecular mechanism

238 With purified components, hnRNP A1 reduces U1 snRNP binding to 5'SSs by binding co

239 k, together with the discovery of E2F3 as an hnRNP-A1-regulated factor, outlines the relevant role pl

240 HnRNP A1 regulates many alternative splicing events by t

241 Here, we provide the mechanism by which hnRNP A1 regulates this event.

242 LANA and identified a cellular RNA helicase, hnRNP A1, regulating the translation of LANA mRNA.

243 During MHV infection, hnRNP A1 relocalizes from the nucleus to the cytoplasm,

244 SF2/ASF and in agreement with other systems, hnRNP A1 repressed c-src splicing in vitro.

245 tide hairpin loop contains the high-affinity hnRNP-A1-responsive 5'-UAGU-3' element and a proximal 5'

246 differentiation, and knockdown of hnRNP L or hnRNP A1 results in the lower induction of Treg cells.

247 RNA immunoprecipitation of hnRNP L and hnRNP A1 revealed a binding motif located central and 3'

248 nformational change to assemble a functional hnRNP A1-RNA complex.

249 Mechanisms and putative hnRNP A1-RNA interactions have been inferred primarily f

250 esented here provide the first insights into hnRNP A1-RNA interactions.

251 ittle is known about the structural basis of hnRNP A1-RNA recognition.

252 esembles sequence elements of several native hnRNP A1-RNA stem loop targets.

253 Here we describe a phosphomimetic mutant of hnRNP A1 (S199E) that is capable of binding both the cyc

254 monstrate that the phosphorylation status of hnRNP A1 serine 199 regulates the AKT-dependent sensitiv

255 hnRNP A1 shuttles between the nucleus and cytoplasm and

256 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) shuttles between the cytoplasm and nucleus and

257 Moreover, interference with hnRNP A1 shuttling activity resulted in downmodulation o

258 ated in CML-BCCD34+ in a BCR/ABL kinase- and hnRNP-A1 shuttling-dependent manner.

259 alorimetric titrations further validate that hnRNP A1-SL3 assembly is complex with the affinity of di

260 rentially spliced gene isoforms in LIN28 and hnRNP A1 small interfering RNA (siRNA)-treated cells.

261 ence of Tra2beta, ASF/SF2 and SRp40, whereas hnRNP A1 specifically inhibits this choice.

262 The physical determinants of hnRNP A1 splice site recognition remain poorly defined i

263 K5 as the kinase responsible for controlling hnRNP A1 subcellular localization in response to hyperto

264 d calorimetric and NMR titrations of several hnRNP A1 subdomains into ESS3.

265 Overexpression of hnRNP A1 suppressed basal gamma-fibrinogen transcription

266 loops represent an important class of known hnRNP A1 targets, yet little is known about the structur

267 Here, we show for hnRNP A1 that interdomain communication is vital for the

268 ein export, even for proteins such as GR and hnRNP A1 that use distinct nuclear export pathways.

269 ) bound to one of its substrates, the NLS of hnRNP A1, that we report here explains the mechanism of

270 Similar to the wild-type hnRNP A1, the hnRNP A1 mutant complexed with an MHV poly

271 However, in contrast to the wild-type hnRNP A1, the mutant protein failed to bind a 250 kDa ce

272 s that regulate the cellular localization of hnRNP A1, the precise mechanism(s), including physiologi

273 the transcription factor E2F3 associates to hnRNP-A1 through a conserved binding site located in the

274 hnRNP A1 thus has been proposed as a host factor in MHV

275 hnRNP L represses CD45 exon 4 by recruiting hnRNP A1 to a sequence upstream of the 5' splice site.

276 into the basis for high-affinity binding of hnRNP A1 to certain RNA sequences, and for nucleic acid

277 ings are relevant to the specific ability of hnRNP A1 to serve distinct roles in post-transcriptional

278 nown exonic splicing regulators, SF2/ASF and hnRNP A1, to the splicing of an exon primarily controlle

279 The formation of p17-hnRNP A1-transportin 1 carrier-cargo complex is required

280 our results reveal that the formation of p17-hnRNP A1-transportin 1 carrier-cargo complex is required

281 This study shows that MARs recruit and bind hnRNP-A1 upon transcriptional up-regulation.

282 , a synthetic high-affinity binding site for hnRNP A1 was also analysed.

283 The importance of hnRNP A1 was demonstrated by induction of apoptosis in e

284 A similar effect of hnRNP A1 was demonstrated with mutant hamster adenine ph

285 RE-specific binding protein in cells lacking hnRNP A1 was purified from CB3 mouse erythroleukemia cel

286 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) was identified as a component of the complexes

287 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), which forms a direct complex with FEN-1 and s

288 by inducing the cytoplasmic accumulation of hnRNP A1, which attenuates internal ribosome entry site-

289 binding site for a known repressor protein, hnRNP A1, which binds to SMN2 but not SMN1 exon 7 RNA.

290 Furthermore, the amount of hnRNP A1, which has been implicated in the export of cel

291 MS patients make antibodies to hnRNP A1, which have been shown to lead to neuronal dysf

292 Furthermore, cellular stress induces hnRNP A1, which is necessary for stress-induced ATF4 pro

293 We show here that hnRNP A1, which shifts splicing towards an upstream 5'SS

294 r ribonucleoproteins [hnRNP]) represented by hnRNP A1, which shuttle continuously between the nucleus

295 tivity) and poly(U)-Sepharose by cytoplasmic hnRNP A1, while nuclear hnRNP A1 binding was unaffected.

296 heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), whose structure was previously solved at 1.75

297 s methylation facilitates the interaction of hnRNP A1 with IRES RNA to promote IRES-dependent transla

298 n the nucleocytoplasmic shuttling pathway of hnRNP A1 with its bound RNAs.

299 This interaction of hnRNP A1 with LANA mRNA could be exploited for controlli

300 phosphorylation modulates the interaction of hnRNP A1 with transportin Trn1.