ライフサイエンスコーパス: human cell

1 Double Strand Break (DSB) yields for plasmid/human cell.

2 t sensor of reactive oxygen species (ROS) in human cells.

3 functions to restrain the immune response in human cells.

4 lin-2 maintains spindle integrity in mitotic human cells.

5 ructures are enriched for endogenous DSBs in human cells.

6 receptors (CCR5 and CXCR4) to gain entry in human cells.

7 eta performs predominantly error-free TLS in human cells.

8 s, and up to 70-fold less toxicity in normal human cells.

9 ntify and quantify target proteins in intact human cells.

10 human MRC-5 cells but only slightly in other human cells.

11 DNA-induced genetic instability in yeast and human cells.

12 es can be low at challenging loci in primary human cells.

13 n-ICL through a unique DSB-free mechanism in human cells.

14 proteins, we activate gene transcription in human cells.

15 RNA and diverse classes of noncoding RNA in human cells.

16 deacetylases (KDACs) have been identified in human cells.

17 at sites of clathrin-mediated endocytosis in human cells.

18 in ticks, has severely impaired function in human cells.

19 aptic circuit has not yet been achieved with human cells.

20 ion factors (TFs) in many different types of human cells.

21 ifically bind to SARS-CoV-2 RNAs in infected human cells.

22 -dependent mRNA translation is suppressed in human cells.

23 autonomous retrotransposon that is active in human cells.

24 ained from rodent HSC to events occurring in human cells.

25 hese viruses with a replicative advantage in human cells.

26 interaction between UBE3A and MEF2C also in human cells.

27 nuate RNA targeting and editing by Cas13a in human cells.

28 engages MPC2 with remarkable selectivity in human cells.

29 d autophagosome closure and HIV-1 release in human cells.

30 ndent deamination in Escherichia coli and in human cells.

31 blastoma protein (Rb) to trigger division in human cells.

32 nscriptional mutagenesis of these lesions in human cells.

33 tion using immunoprecipitation approaches in human cells.

34 reduced P-gp and BCRP transport activity in human cells.

35 man immunodeficiency virus type 1 (HIV-1) in human cells.

36 i returned TEER to background levels only in human cells.

37 t versus IRES-mediated translation in living human cells.

38 ct alpha-satellite RNA transcripts in intact human cells.

39 onses, a result conserved with HSC70 S153 in human cells.

40 related with non-CpG methylation patterns in human cells.

41 by simulating DNA rereplication in yeast and human cells.

42 NLRP3 inflammasome activation in murine and human cells.

43 ike DfCas9, the PpCas9 nuclease is active in human cells.

44 epair and other aspects of DNA metabolism in human cells.

45 hich results in reduced heme biosynthesis in human cells.

46 ta in bypassing alkylated guanine lesions in human cells.

47 nuclease, allowing more flexible editing in human cells.

48 nce and 697 variants on activity in cultured human cells.

49 tes the release of virus from FIV-expressing human cells.

50 effects of antileishmanial drugs on primary human cells.

51 on of kinetochore-microtubule attachments in human cells.

52 toxicity in yeast and TDP-43 aggregation in human cells.

53 py-neutral loss of heterozygosity (cnLOH) in human cells.

54 tegration at safe harbor loci in porcine and human cells.

55 er chromatin and gene expression profiles of human cells.

56 nity IgE receptor (FcepsilonRI) signaling in human cells.

57 for the dissection of ribosome biogenesis in human cells.

58 particularly in AT-rich sequence contexts in human cells.

59 activity on rat P2X2 receptors expressed in human cells.

60 n of ADP-ribose moieties after DNA damage in human cells.

61 activated in response to metabolic stress in human cells.

62 any important physiological processes within human cells.

63 m endosomes and decreased PS-ASO activity in human cells.

64 y treatment is predictive of the response in human cells.

65 (CDC20)-dependent destruction of cyclin B in human cells.

66 cription activity and CTD phosphorylation in human cells.

67 nd were proposed to differ between mouse and human cells.

68 distinct regulation of these three genes in human cells.

69 hanisms by which SARS-CoV-2 enters and exits human cells.

70 ructural aberrations in large populations of human cells.

71 motes STRIPAK-mediated inhibition of MST2 in human cells.

72 ansfers isolated mitochondria into mouse and human cells.

73 PARP2, are early responders to DNA damage in human cells(1,2).

74 As in human cells, 3' uridylation of histone mRNA is induced u

75 n improved bioreactor design which maintains human cells alive and metabolically active for up to 72

76 Because human cells also use Arp2/3-dependent lamellar protrusio

77 EM16A potentiator (ETX001) were evaluated in human cell and animal models of airway epithelial functi

78 high-density DNase I cleavage maps from 243 human cell and tissue types and states and integrated th

79 etitive fitness, and transmission in primary human cells and animal models.

80 Analyses in human cells and diabetic mice with cardiomyocyte-specifi

81 mediated indel formation and base editing in human cells and enabled A*T-to-G*C base editing of a sic

82 some is the microtubule organizing center of human cells and facilitates a myriad of cellular functio

83 homologous recombination (HR) DNA repair in human cells and functions as a tumor suppressor in mice.

84 HTT protein levels in mouse striatal cells, human cells and HD mouse models.

85 xpressed 26 of the 29 SARS-CoV-2 proteins in human cells and identified the human proteins that physi

86 re, we analyzed hetero-oligomer formation in human cells and in vitro using purified proteins.

87 s that lack ubiquitination are attenuated in human cells and in wild-type mice, but not in live mosqu

88 subset of insertion events in both mouse and human cells and is coincident with the presence of high

89 f the "myxovirus resistance proteins" MxA in human cells and its ortholog Mx1 in murine cells.

90 es on a wide range of sites with NRN PAMs in human cells and lower but substantial activity on those

91 anine erythrocytes, intermediate for rat and human cells and lowest for bovine cells.

92 endogenous substrates of the RQC pathway in human cells and provide insight into common principles c

93 of STRIPE-seq to TSS profiling in yeast and human cells and show that it can also be effectively use

94 o-Seq datasets from virus-infected yeast and human cells and showed that virus CUB trans-regulated tR

95 t C-NHEJ promotes deletion rearrangements in human cells and that cell type-specific differences in t

96 able and reversible, transparency for living human cells and tissues has remained elusive to date.

97 minated in most, both healthy and malignant, human cells and tissues studied.

98 ese viruses are restricted to replication in human cells and tissues, making them difficult to study

99 also describe novel ex vivo methods based on human cells and tissues, such as engineered heart tissue

100 ns unaffected by transcription inhibition in human cells, and by facilitating accurate differential p

101 Equivalent processes may occur in human cells, and cause neurological disease when impaire

102 e B viruses can recombine to gain entry into human cells, and confirm that human ACE2 is the receptor

103 , primary, differentiating, and immortalized human cells, and demonstrate that a class of origins, te

104 ing data from model organisms and engineered human cells, and show that it can be used to improve the

105 that Z-DNA is mutagenic in yeast as well as human cells, and that the nucleotide excision repair com

106 profiles during EV-A71 infections in primary human cells, and the potential involvement of TREM-1 in

107 r chemical modifications, directly in living human cells, and therefore has great potential in the co

108 strated in neuroblastoma cell lines, primary human cells, and xenografts.

109 hosphorylation sites have been identified in human cells, approaches to determine the functional impo

110 he secretion of ADAM10-bearing exosomes from human cells as well as in mice.

111 detect loops that were previously missed in human cells as well as loops in other organisms.

112 n in diabetic and insulin resistance-induced human cells, as well as in mice fed with high-fat chow;

113 ectively, and knocked down specific mRNAs in human cells at a level of ~25%.

114 odel in which Pol delta promotes Alt-NHEJ in human cells at DSBs, including translocations.

115 nation (IHR) occurs spontaneously in somatic human cells at frequencies that are low but sufficient t

116 We benchmark Cumulus on the Human Cell Atlas Census of Immune Cells dataset of bone

117 The Human Cell Atlas consortium is emerging as an important

118 cell genomics, imaging technologies, and the Human Cell Atlas initiative have together enabled a syst

119 ully used to generate reference maps for the human cell atlas.

120 We generated human cell atlases of chromatin accessibility and gene e

121 r fibrosis model, as well as novel 2D and 3D human cell-based co-culture of human hepatocytes, KCs (K

122 life, identifiable viruses that replicate in human cells become more prominent.

123 Preventing phosphorylation of S183/S185 in human cells boosted p53 activity and allowed tumor cells

124 80 not only accentuates DNA damage levels in human cells but also adversely affects the cell cycle ch

125 n vitro, HPG is capable of infecting bat and human cells, but not mouse cells, and displays a similar

126 Entamoeba histolytica kills human cells by ingesting fragments of live cells until t

127 tor of fetal gamma-globin gene expression in human cells by repressing BCL11A transcription.

128 Normal human cells can either synthesize cholesterol or take it

129 In summary, our findings uncover how human cells can exploit remnants of once-infectious retr

130 immunocompetent mice, as well as transgenic human cells carrying an inducible gene circuit for the o

131 Genomic deletion of one FMRE in human cells caused proliferative deficiencies and transc

132 poptosis in JAK2-dependent mouse and primary human cells, causing regression of the malignant clones

133 dly generate tyrosine-tRNA(GUA) fragments in human cells-causing significant depletion of the precurs

134 ntal lineage tracing dataset to date, 34,557 human cells continuously traced over 15 generations, and

135 st complex and energy demanding processes in human cells, critical for cell growth and proliferation.

136 of Abeta (Abeta40 and Abeta42) in a primary human cell culture model.

137 fe human exposure cohorts, a mouse model and human cell culture to test our hypothesis.

138 In human cell culture, bacterial SLs are processed by host

139 Competence with human cell culture, tissue processing, immunohistology a

140 In addition, Leg5,7Ac(2)-fed human cells did not display this NulO on their surface.

141 , which aims to track, understand and target human cells during the onset and progression of complex

142 reased, and senescent CD8 T cells decreased (human cells); effects were generally maintained in the e

143 enesis in an MGEP model system consisting of human cells eliciting <3% productive dengue infection.

144 Enriched astrocyte-biased human cells enable future experiments to determine the s

145 The gene regulatory network (GRN) of human cells encodes mechanisms to ensure proper function

146 For >130 days, the device supported human cells engineered to secrete erythropoietin in immu

147 RHBDL2 in giant plasma membrane vesicles of human cells even at concentrations that highly exceed en

148 DALRD3-deficient human cells exhibit nearly complete loss of the m3C modi

149 y, TFEB is mislocalized and downregulated in human cells expressing GGGGCC repeats and in C9-ALS pati

150 events that lead to a DNA expansion event in human cell extracts.

151 In human cells, FACT depletion extends dCas9 residence time

152 well as subcellular changes) that accompany human cell fate transitions.

153 trum inhibition of distinct Cas9 proteins in human cells (for example, SpyCas9, SauCas9, SthCas9, Nme

154 y, PAC-MAN (prophylactic antiviral CRISPR in human cells), for viral inhibition that can effectively

155 Here, using newly developed human cell-free systems that recapitulate distinct inter

156 asthma-associated factors are attenuated in human cells from asthma subjects when exogenous SP-A is

157 tects not only NMR cells, but also mouse and human cells from stress-induced cell-cycle arrest and ce

158 e identification of their targets in primary human cells has been challenging.

159 manipulation that is not easily available in human cells, has been used to characterize the cellular

160 es in mice are different from humans in that human cells have a natural mutation in the alpha1,3 gala

161 blocking the site on the virus used to bind human cells have been identified.

162 editing and RNAi-mediated gene silencing in human cells, here we analyzed the consequences of PDS5 d

163 To examine such rearrangements in human cells, here we developed a reporter assay based on

164 double-strand break (DSB) repair pathways in human cells, how DNA repair failures can lead to human d

165 In all human cells, human leukocyte antigen (HLA) class I glyco

166 y factor in all tested cell types, including human cells, I/LnJ CD300LF does not function as an MNV e

167 tethered at molecular distances to the ER in human cells in a tunable fashion.

168 ion of eight upregulated LTRs into recipient human cells in culture showed robust and heterogenous ac

169 independently of adhesive cues in flattened human cells in culture.

170 act of such antileishmanial drugs on primary human cells in relation to immune function.

171 stigate the interphase chromatin dynamics in human cells in response to local DNA damage, specificall

172 dan bat, could mediate infection of Vero and human cells in the presence of exogenous trypsin.

173 as investigating the action of IRL201104 on human cells in vitro.

174 We found that all Arfs expressed in human cells, including Arf6, normally associated with th

175 s behind these functions in mouse models and human cells, including interactions with other TFs, and

176 Despite the ~90 DUBs in human cells, including two others in addition to UCHL5 a

177 E2, consistent with 2019-nCoV's capacity for human cell infection.

178 s of DNA double-strand break (DSB) repair in human cells involve the MRE11-RAD50-NBS1 (MRN) complex a

179 Understanding how SARS-CoV-2 enters human cells is a high priority for deciphering its myste

180 hat ZCWPW1 has co-evolved with PRDM9 and, in human cells, is strongly and specifically recruited to P

181 es demonstrating C5aR2 can modulate C5aR1 in human cells, it is not yet known whether C5aR2 functiona

182 Human cells lacking Skd3 exhibit reduced solubility of v

183 Telomere shortening in human cells leads to a DNA damage response that signals

184 ed Dicer repression in zebrafish embryos and human cells leads to increased canonical miRNA productio

185 Deleting such candidate TEs in human cells leads to the collapse of conserved loop and

186 events, CHyMErA identifies exons underlying human cell line fitness.

187 The human cell line HEK293 has bio-synthetic potential for h

188 sing a genome-wide CRISPR/Cas9 screen in the human cell line PLB-985.

189 urate 21-plex quantification of labeled K562 human cell line protein digests via single-shot nanoLC-M

190 severe NDDs and subsequent confirmation in a human cell line revealed interactions between UBE3A/Ube3

191 f the ribosomal proteins RPS25 or RACK1 in a human cell line, as both proteins are implicated in dire

192 On the effectively haploid CHM13 human cell line, HiCanu achieved an NG50 contig size of

193 D-dependent control of ITGB7 expression in a human cell line.

194 cal approaches to analyze a MT-CYB-deficient human cell line.

195 e occupancy and methylome (nanoNOMe) on four human cell lines (GM12878, MCF-10A, MCF-7 and MDA-MB-231

196 pensive transcriptomic profiles derived from human cell lines after chemical compound treatment to tr

197 entary in vitro studies were performed using human cell lines and a murine organoid system.

198 affected these pathways in various cultured human cell lines and in mouse embryonic fibroblasts.

199 n this issue of the JCI, Palrasu et al. used human cell lines and mouse models to provide mechanistic

200 Using human cell lines and mouse models, we found that elevate

201 data and showed efficacy in a broad range of human cell lines and primary AML cells from patients.

202 We show that HUSH-depletion in human cell lines and primary fibroblasts leads to induct

203 ing SEMA6A/6B expression in several distinct human cell lines and primary human endothelial cells res

204 AB569 was not toxic to human cell lines at bactericidal concentrations using a

205 We generated a large library of human cell lines deficient in a particular BBSome subuni

206 l rearrangements of the respiratory chain in human cell lines depleted of the catalytic complex IV su

207 We used CRISPR-Cas9 to engineer human cell lines expressing POLE tumor variants, with an

208 leverages the genetic diversity of multiple human cell lines to highlight viral determinants that co

209 f 684 TFs and cofactors assayed across a 117 human cell lines under a multitude of growth and mainten

210 acterize large libraries of GPCR variants in human cell lines with a barcoded transcriptional reporte

211 transferrin receptor 1 (TfR1) in established human cell lines with a designer disulfide FRET probe.

212 further describe how OOPS can be applied in human cell lines, Arabidopsis thaliana, Schizosaccharomy

213 s of cytoplasm, nucleoplasm, and nucleoli of human cell lines, challenged by various perturbations.

214 Here, using human cell lines, fluorescence microscopy, and pulldown

215 compared to commercial controls for several human cell lines, including HeLa, HEK 293T, K562, and ke

216 as antibody-mediated inhibition of IL2RA in human cell lines, mouse models, and primary patient samp

217 ed in this review have proven efficacious in human cell lines, patient-derived cells, and pre-clinica

218 e number of distinct biological functions in human cell lines, such as four different types of chroma

219 We model the interaction in 58 human cell lines, where IFN-gamma in vitro exposure lead

220 ttenuation of HSV-1 replication in mouse and human cell lines.

221 gene of MVA enhances replication in numerous human cell lines.

222 tium and essentiality screens carried out on human cell lines.

223 n were found to be different in mouse versus human cell lines.

224 predict the structural ensembles of multiple human cell lines.

225 s and edges labeled with chromatin states in human cell lines.

226 iral infection are different in mouse versus human cell lines.

227 /3 complex, reduced viral replication in two human cell lines.

228 OV and MARV during infection of both bat and human cell lines.

229 witching of genomic compartments observed in human cell lines.

230 in profiling of alpha-mannosidases from both human cell lysate and mouse tissue extracts.

231 ulfuramidimidoyl fluoride electrophile, with human cell lysate, and the protein conjugates formed wer

232 directly analyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the

233 Entamoeba histolytica acquires and displays human cell membrane proteins, enabling immune evasion.

234 s and quantitation of O-glycans derived from human cell membrane.

235 ying large-scale nuclear architecture across human cell nuclei in interphase.

236 We successfully cultured human cells on PLA substrates and devices, without coati

237 s is in stark contrast to what we observe in human cells or T. thermophila, an organism with similar

238 extensions, including the release of primary human cells or the design of the paper-based diagnostic,

239 -end-directed microtubule-based transport in human cells, performing functions that range from retrog

240 as powerful proxies for difficult to obtain human cell populations, facilitating the illumination of

241 and non-invasive method of isolating viable human cells present at the site of skin wounds.

242 Disruption of these events in human cells prevented the recruitment of downstream ESCR

243 OCRL-depleted human cells proliferated more slowly and about 10% of th

244 e cytotoxicity and high biocompatibility for human cells, promising a wide range of bio applications,

245 dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein prec

246 However, defining this architecture in human cells remains challenging because of the large siz

247 derlying this dysregulation, particularly in human cells, remains poorly understood.

248 hat increased purinosome assembly in hypoxic human cells requires the activation of hypoxia inducible

249 efficiency of ~40% and ~20% in bacterial and human cells, respectively, and knocked down specific mRN

250 re, we show that the elimination of TRIM6 in human cells results in an increase in WNV replication an

251 transient overexpression of the kinase PLK4, human cells return to a normal centriole number during t

252 hagocytosis assays in MPO-deficient mice and human cells revealed altered neutrophil function and imp

253 based on the formation of stress granules in human cells, revealed cell cycle-associated kinases as m

254 ncogenic Ras than mouse, blind mole-rat, and human cells, revealing suppressed Ras signaling as an an

255 We found surprisingly that, in yeast and human cells, RNA abasic sites are prevalent.

256 nts cyclin B1 localization to centrosomes in human cells, suggesting that this mechanism of cyclin-CD

257 cies was less pronounced than in transformed human cells, suggesting that transformation may impact a

258 We executed an interactome screen of 564 human cell-surface and secreted proteins, most of which

259 n and mouse ROR2 but not human ROR1 or other human cell-surface antigens.

260 Further, neighboring introns in human cells tend to be spliced concurrently, implying th

261 gure its active site entirely differently in human cells than in the purified Pol establishes a new p

262 e developed a hepatic organoid platform with human cells that can be used to model complex liver dise

263 We further find in human cells that loss of RAZUL by CRISPR-based gene edit

264 Finally, we provide evidence of human cells that phenocopy murine CD122+Macs in secretor

265 of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides tha

266 Moreover, in human cells the loss of H3K9me3 leads to an increase in

267 Our results reveal that, in yeast and human cells, there are RNA abasic sites, and we identify

268 eria, and later by viruses that replicate in human cells; this second phase is modulated by breastfee

269 er allows several lineage B viruses to enter human cells through an unknown receptor.

270 en successfully corrected in mice, dogs, and human cells through CRISPR/Cas9 editing.

271 physiological and pathological processes in human cells; thus, our results provide a structural basi

272 s, large-animal models, ex vivo studies with human cells/tissues, and new delivery techniques will li

273 est-resolution maps of chromosome folding in human cells to date, providing a valuable resource for s

274 we use live-cell single-molecule imaging in human cells to determine rate constants of the AGO2 clea

275 Upon exposure of human cells to DNA-damaging agents, NUCKS1 controls the

276 ity to form virus-like particles (VLPs) from human cells to form a competent system for BSL-2 studies

277 we performed a massively parallel screen in human cells to identify loss-of-function missense varian

278 proteins together promote MVA replication in human cells to levels that are comparable to titers in c

279 onsive regulatory network operates in single human cells to process repetitive IFN stimulation.

280 teractions using multi-contact 3C (MC-3C) in human cells to provide insights into the topological ent

281 d that CRISPR/Cas9-mediated GALE deletion in human cells triggers major imbalances in NSs and dramati

282 owever, here we found that when expressed in human cells, two highly homologous HSP70s, HSPA1A and HS

283 housands of putative REs specific for either human cell type.

284 in landscape underlying the specification of human cell types is of fundamental interest.

285 iched proteins, generated from 104 different human cell types or tissues.

286 , and analysed gene expression in 24 diverse human cell types, including core ENCODE cell lines.

287 of mediating ADCC responses against infected human cells undergoing lytic reactivation.

288 enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA p

289 We investigated the infection of human cells using Chikungunya virus (CHIKV) and influenz

290 tom engineered circuits stably integrated in human cells using CRISPR.

291 te-specific recombination and RMCE assays in human cells using native 'attB' sites.

292 eening for brightness and expression rate in human cells, we developed mGreenLantern, a fluorescent p

293 Using CENP-A genetic inactivation in human cells, we directly interrogate if differences in t

294 To investigate IAV tropism in human cells, we evaluated the replication of IAV strains

295 In cultured human cells, we find that misfolded proteins can directl

296 In human cells, we observe robust inhibition of SauCas9-ind

297 ) and minor-groove N (2)-alkyl-dG lesions in human cells, where the alkyl groups are ethyl, n-butyl (

298 understanding of DNA DSB repair pathways in human cells will lead to novel therapeutic strategies to

299 of purified LOXCAT to the medium of cultured human cells with a defective electron transport chain de

300 Moreover, titrating human cells with a G4 ligand alters the ability of ribos