ライフサイエンスコーパス: hybridoma

1 imed IL-2 release from Ag85B-specific T cell hybridoma.

2 ant H(V)1 channels in LK35.2 cells, a B cell hybridoma.

3 albumin to a SIINFEKL-specific CD8(+) T-cell hybridoma.

4 riable fragment (scFv) cloned from the HMFG2 hybridoma.

5 ous IgG switch mutants from an anti-Gal IgG1 hybridoma.

6 extent than the parental Ab produced by the hybridoma.

7 hologically at day 21 after injection of the hybridoma.

8 helial IC deposition, as compared with NEP25/hybridoma.

9 in-specific and DR4 allele-restricted T cell hybridoma.

10 se splenocytes and a CD1d-positive mouse NKT hybridoma.

11 ional R-mAbs from a non-viable cryopreserved hybridoma.

12 ltered the duration of signaling in a T cell hybridoma.

13 ation of insulin to a Qa-1-restricted T cell hybridoma.

14 nimals without the need to make conventional hybridomas.

15 dues 62-70) to murine, DQ2-restricted T cell hybridomas.

16 cell lines, did not activate mouse iNKT cell hybridomas.

17 d in 2 different TCR alpha(-)/beta(-) T cell hybridomas.

18 or MHC class I and II presentation in T cell hybridomas.

19 or murine class II MHC-restricted CD4 T cell hybridomas.

20 II to antigen-specific MHC-restricted T cell hybridomas.

21 omplexes that could directly activate T cell hybridomas.

22 ters) of mAb-producing cell lines, including hybridomas.

23 specific B cell lines prior to generation of hybridomas.

24 hnologies of fluorescence flow cytometry and hybridomas.

25 that stimulated various dNKT, but not iNKT, hybridomas.

26 One of these hybridomas, 260.8, produced a monoclonal antibody that r

27 A total of 73 anti-Dsg hybridomas (47 IgM and 26 IgG) were isolated.

28 d binding of the tetramer to a number of the hybridomas, a significant percentage remained unstainabl

29 study, we report that certain murine B cell hybridomas accumulate intracellular IgM and release larg

30 T cell hybridoma activation was measured by enzyme-linked immun

31 T cell hybridomas against an immunogenic B. pseudomallei FliC e

32 Screening of these T cell hybridomas against IKEPLUS and ribosomes enriched from I

33 Hybridoma (AGS-22M6E) and CHO (ASG-22CE) versions of enf

34 reover, after transduction into the 2 T cell hybridomas, all 4 were functional as evidenced by their

35 Strikingly, pre-FS hybridomas also exhibit evidence of antigen selection, i

36 g of the F240 mRNAs produced in the parental hybridoma and CHO cells revealed identical sequences, su

37 educing and enhancing mutants using a T cell hybridoma and found similar reducing and enhancing effec

38 es Fas death receptor expression in a T cell hybridoma and human PBL.

39 chains were isolated from the corresponding hybridoma and inserted into a replication-defective sero

40 rotein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum

41 we present a platform process that combines hybridoma and morphogenics technologies for the generati

42 gondii-specific, lacZ-inducible, CD4 T cell hybridoma and used it as a probe to screen a T. gondii c

43 anized control rAbs derived from anti-myelin hybridomas and anti-myelin monoclonal antibodies readily

44 Hybridomas and cDNA clones derived from the immunized mi

45 antibody sequences from retrovirus-specific hybridomas and GC B cells from infected mice revealed Ig

46 e used functional screening of anti-human AM hybridomas and isolated a mAb, PLK-1, which inhibits AM

47 1d levels more efficiently activate NKT cell hybridomas and primary NKT cells independently of whethe

48 different purposes, including generation of hybridomas and reprogramming of somatic cells.

49 onses using T. gondii-specific CD8(+) T cell hybridomas and splenic CD8(+) immune T cells from chroni

50 lls, we generated spontaneous splenic B cell hybridomas and used a novel microscopy screen to detect

51 uces protective antibody, the IgG3-producing hybridoma, and a nonprotective IgG1-producing hybridoma

52 g target specific antibodies from bacterial, hybridoma, and B cell libraries.

53 le of activating an OVA-specific CD8+ T-cell hybridoma, and that this phenomenon is dependent on the

54 identified, characterized with cloned T-cell hybridomas, and confirmed in tetramer and ELISpot assays

55 ificity of VSG-specific T-cell lines, T-cell hybridomas, and Th cells activated during infection.

56 in the ability of the tetramer to stain the hybridomas, and there was a strong correlation between t

57 e seems to be biased, particularly among IgG hybridomas, and third, most hybridomas are mutants and e

58 Also found among 56R/kdel hybridomas are clones that have inactivated the H chain

59 ularly among IgG hybridomas, and third, most hybridomas are mutants and exhibit a bias in favor of CD

60 studies demonstrate that in a murine T cell hybridoma as well as in primary murine thymocytes, a fra

61 les of Jbeta1(DJbeta/omega) alphabeta T cell hybridomas, as compared with on the Jbeta1(omega) allele

62 C class I and MHC class II-restricted T cell hybridomas, as well as enhanced a Th2-like response by i

63 could cause some anti-HCV-antibody-producing hybridoma B cells to make less-protective antibodies.

64 In this study, a hybridoma based biosensor was developed for rapid, sensi

65 efficiently stimulated virus-specific T cell hybridomas but failed to induce alloreactive stimulation

66 When expressed in a T-cell hybridoma by retroviral vector, MS4a4B protein constitut

67 The time required to isolate hybridomas by a limiting serial-dilution, however, has r

68 Stimulation of dNKT hybridomas by microbial PG was independent of Toll-like

69 ion of 2B11.3 hybridoma to NEP25 mice (NEP25/hybridoma) caused IC deposition limited to the subendoth

70 Finally, hybridoma CD4(+) T clones derived from DBA/2 recipients

71 tional screening of up to 300,000 individual hybridoma cell clones within less than a day.

72 munosorbent assay (ELISA) for rat IgG from a hybridoma cell culture.

73 The application of whole hybridoma cell directly as a sensing element in biosenso

74 xamined by using an I-A(b)-restricted T-cell hybridoma cell line (BB7) that recognizes an epitope der

75 Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody spec

76 yc antibodies harvested from a cultured 9E10 hybridoma cell line without the need for further purific

77 alpha-GalCer was comparable to a murine iNKT hybridoma cell line.

78 ion technology, we generated 6 stable hetero-hybridoma cell lines from EBV-transformed B-CLL cells, a

79 Hybridoma cell lines were generated and compared for bin

80 d mononuclear cells] and epithelial and iNKT-hybridoma cell lines) have been used to determine the im

81 ese antibodies were spiked into an unrelated hybridoma cell population in a ratio of 1:10,000 we obse

82 e expression of the CD4 gene in CD4-positive hybridoma cells and double-positive thymocytes.

83 G (IgG) variable domains from cryopreserved hybridoma cells and input them into an integrated pipeli

84 ted cells to both M. tuberculosis-specific T hybridoma cells and naive P25 M. tuberculosis T-cell rec

85 ion: in order to detect antigen specificity, hybridoma cells are incubated with a hapten-horseradish

86 ak stimulation to M. tuberculosis-specific T hybridoma cells but not naive P25 T cells.

87 he first time in present study suggests that hybridoma cells could provide a valuable tool for future

88 ogenics, could be used to improve suboptimal hybridoma cells generated by means of ex vivo immunizati

89 njected with viable MHC-incompatible 3F7.A10 hybridoma cells grown in serum-free medium mounted stron

90 Moreover, these hybridoma cells have proven suitable for genetic optimiz

91 Here we describe a system in cultured hybridoma cells in which transcription of the endogenous

92 ransduced into either TCRalphabeta-deficient hybridoma cells or Rag1-/- bone marrow progenitor cells.

93 inemia was induced by i.p. injection of 6-19 hybridoma cells producing an IgG3 cryoglobulin with rheu

94 Hundreds of labeled hybridoma cells producing monoclonal antibodies (mAbs) s

95 tuberculosis-specific CD4+ T cells or F9A6 T hybridoma cells specific for M. tuberculosis antigen (Ag

96 the folded MR1 conformer activated 2/2 MAIT hybridoma cells tested, 3) the pattern of MAIT cell acti

97 MS-1-secreting hybridoma cells were then transferred into the peritoneu

98 sor was constructed by transfecting specific hybridoma cells with aequorin reporter gene and the biol

99 15 min by co-compartmentalizing single mouse hybridoma cells, a fluorescent probe and single beads co

100 reens require the generation of immortalized hybridoma cells, as well as clonal expansion in microtit

101 detection of antibody secretions from single hybridoma cells, the enhanced time resolution revealed t

102 5HT2c serotonin receptor and derived clonal hybridoma cells, which we tested for specific antigen re

103 microarray expression profiling of 3B4.15 T-hybridoma cells.

104 to present exogenous antigenic peptide to T hybridoma cells.

105 ogenic T cells to Ag paralleled those of the hybridoma cells.

106 (IS) formation by CD3-specific Ab expressing hybridoma cells.

107 exogenous lipids to CD1d-restricted Valpha14 hybridoma cells.

108 ion in CD3zeta-deficient MA5.8 murine T-cell hybridoma cells.

109 milar efficiencies for presentation to BB7 T hybridoma cells.

110 ng and MHC-II presentation, as detected by T hybridoma cells.

111 novel hapten-specific labeling technique of hybridoma cells.

112 y HIV-1-infected individuals and produced by hybridoma cells.

113 r mobility than CD8alpha-bound Lck in OT-I T hybridoma cells.

114 ecting antibodies secreted from single mouse hybridoma cells.

115 First, clonally related sets of anti-DSG1 hybridomas characterize the response in individual FS pa

116 y genetic structure of hp-B6.1, the original hybridoma clone (ori-B6.1) stored frozen since 1995, a s

117 njury model NEP25 mice and an IgG3-producing hybridoma clone 2B11.3 established by MRL/lpr mice, the

118 as completely abrogated by anti-alpha4beta7 (hybridoma clone DATK32) or anti-alpha4 integrins (PS/2).

119 y used to block VLA-4 function in the mouse (hybridoma clone PS/2) is not specific to VLA-4 but inhib

120 After generation of hybridoma clones and assessment of their binding and fun

121 A substantial proportion of hybridoma clones generated from L2pB1 cells reacted to d

122 To test this hypothesis, we infected human hybridoma clones producing either neutralizing or non-ne

123 asurements of the supernatants of the sorted hybridoma clones revealed that all hapten-specific hybri

124 oma clones revealed that all hapten-specific hybridoma clones secrete antibodies against the target.

125 T-selection and cloning, we established nine hybridoma clones secreting anti-apoA-I mAbs in which fou

126 luding increased yield of antibody-producing hybridoma clones, ensured monoclonality of sorted cells,

127 All of the hybridoma clones, except for a neutralizing antibody-pro

128 Purified IgEs from those hybridomas could activate a basophilic cell line to unde

129 except for a neutralizing antibody-producing hybridoma, could be infected with HCV and support virus

130 e used in vitro IEC binding assays to screen hybridomas created from B cells of unmanipulated wild-ty

131 The use of FBS in hybridoma culture media is examined here, with regards t

132 ols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface pla

133 1E1 is produced in hybridoma cultures as a mixture of monomers, dimers, and

134 uccessful in silico affinity maturation of a hybridoma derived antibody, AB1, using just a homology m

135 Using a unique panel of human hybridomas derived from memory B cells after pneumococca

136 The mAbs were generated in hybridomas derived from mice vaccinated with a fentanyl

137 hich is expressed by almost all IgG anti-DNA hybridomas derived from the glD42H mouse.

138 A panel of hybridoma-derived anti-EpCAM mAbs was generated and scre

139 Vaccination with hybridoma-derived autologous tumor immunoglobulin (Ig) i

140 Vaccination with patient-specific hybridoma-derived Id vaccine after chemotherapy-induced

141 kinetic profile of either transfectoma- or a hybridoma-derived ipilimumab.

142 In this work, the crystal structures of the hybridoma-derived PFA1 Fab in complex with pyro-Glu3-Abe

143 protocols, we eliminated aberrant Sp2/0-Ag14 hybridoma-derived variable light transcripts using restr

144 Importantly, nearly 30% of the hybridomas did not stain with tetramer, and these cells

145 -/- mice with and without subcutaneous 260.8 hybridomas disclosed that this IgA did not affect B. the

146 e performed with use of the murine iNKT cell hybridoma DN32.D3.

147 tion and 2) to minimize subsequent laborious hybridoma efforts by positive selection of Ag-specific,

148 Using collagen-specific T cell hybridomas engineered to overexpress either Syk, Zap-70,

149 is of TCR expression showed that >90% of the hybridomas expressed the same TCR beta-chain variable re

150 nse, we analyzed 22 MOG35-55-specific T cell hybridomas expressing distinct TCR.

151 ited anti-70k T cell proliferation and bound hybridomas expressing the conserved CDR3 motifs.

152 antibody requires retrieval of an individual hybridoma from polyclonal mixtures of cells producing an

153 t repertoire of V(H) usage in IgM anti-dsDNA hybridomas from AID-deficient mice suggests that there i

154 We have previously shown that anti-DNA hybridomas from diseased New Zealand Black/New Zealand W

155 ity complex (MHC) class II-restricted T cell hybridomas from IKEPLUS-immunized mice.

156 i-PA) and IQNLF (anti-LF), were developed as hybridomas from individuals immunized with licensed anth

157 The majority of hybridomas from lungs, but not from spleens, responded t

158 ain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transg

159 The analysis of hybridomas from these mice revealed that the only avenue

160 of Ag-specific, Ab-secreting cells prior to hybridoma fusion and validation screening.

161 BALB/c-derived T-cell hybridomas generated against this region were CD3(+), CD

162 Four-way primary screening of 763 hybridomas generated from mice immunized with guanosine

163 Most hybridomas generated from the proliferating cells are po

164 ABX-CBL is a hybridoma-generated murine IgM monoclonal antibody again

165 In the subset of patients who received hybridoma-generated vaccines, we found that anti-idiotyp

166 After immunization and hybridoma generation, a collection of 20 mouse monoclona

167 ic sorting using the same tetramers prior to hybridoma generation.

168 es (cDbs) derived from the parental antibody hybridomas GK1.5 and 2.43, respectively, for (89)Zr-immu

169 intra-islet-derived anti-peripherin-reactive hybridoma H280.

170 The V(H) sequences in the infected hybridomas had a significantly higher mutation frequency

171 have since found that a highly passaged B6.1 hybridoma (hp-B6.1) resulted in antibody that has little

172 stimulate the sulfatide-reactive type II NKT hybridoma Hy19.3 in a CD1d-dependent manner.

173 Comparison of the hybridomas, identical except for TCR sequence, revealed

174 s I MHC ligand were transduced into a T cell hybridoma in the absence or presence of coreceptors.

175 SF to both the HC gp-39 and human CII T cell hybridomas in an IFNgamma-dependent and MHC-restricted m

176 control rAbs were generated from anti-myelin hybridomas in which murine variable region sequences wer

177 lly, introducing the CD4 coreceptor into the hybridomas increased the proportion of cells that could

178 late-bound PG/CD1d complexes stimulated dNKT hybridomas, indicating direct recognition by the dNKT ce

179 e random TCR repertoires in recipient T cell hybridomas, interrogated their reactivities to antigen-p

180 V(H) gene segment use by IgM hybridomas is diverse, but is restricted among IgG hybri

181 eported that the Ab F240, when produced in a hybridoma, is nonneutralizing as assessed by standard ne

182 Individual hybridomas isolated from the spleen of a 10-month-old BX

183 Valpha14Jalpha18 T-cell receptor-expressing hybridoma line by dendritic cells that is CD1d-dependent

184 els at the single-cell level within a single hybridoma line was observed and high expressors could be

185 ein endothelial cells and their immortalized hybridoma line, EA.hy926.

186 We report the generation of six murine hybridoma lines producing two IgM and four IgG1 MAbs to

187 We were able to generate hybridoma lines secreting mAbs with high binding specifi

188 od and hepatic mononuclear cells, mouse MAIT hybridoma lines, HLA-DR4-transgenic mice, MAIThighHLA-DR

189 ma mice with sublytic podocyte injury (NEP25/hybridoma/LMB2) resulted in increased glomerular CFH exp

190 s dominantly expressed in podocytes of NEP25/hybridoma/LMB2.

191 ponses to BCR ligation and, when captured as hybridoma mAb lines, maintain their dual (gp41/lipid) af

192 uch as conditioned cell culture supernatant, hybridoma media, and mouse ascites fluid.

193 diotype proteins were produced either by the hybridoma method or by expression of recombinant idiotyp

194 mory B cells of a healthy individual using a hybridoma method.

195 ross-present exogenous male Ag to the T cell hybridoma, MHH, specific for HYUty plus D(b).

196 NEP25/hybridoma mice with sublytic podocyte injury (NEP25/hybr

197 In a mouse T cell hybridoma model, TNF attenuated T cell receptor (TCR) si

198 r from commercial sources or during a rabbit hybridoma monoclonal screening and selection process usi

199 urface protein A (PspA) to a PspA-specific T hybridoma more efficiently than macrophages.

200 We showed previously that in a CD8(+) T cell hybridoma, nonstimulatory endogenous peptides enhance T

201 lonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the

202 f whether it has been generated via standard hybridoma or display methods.

203 with more labor-intensive and time-consuming hybridoma or recombinant DNA methods.

204 n recognized as agonists by Treg-derived TCR hybridomas or CD4+CD25+ thymocytes, containing both natu

205 Antibody discovery typically uses hybridoma- or display-based selection approaches, which

206 In vitro treatment of mouse hybridoma (PA2.1) cultures with antiYIL-6R decreased IgG

207 ns undergo receptor editing, we have studied hybridoma panels from 56R/kappa-deleted (kdel) mice.

208 and rapid assay was developed using a murine hybridoma Ped-2E9 cell model.

209 After investigation on hybridoma performance, the biosensor was constructed by

210 ibodies were isolated from phage display and hybridoma platforms by functional screening for opsonoph

211 These hybridomas predominantly used the TCR V(beta)14 and V(be

212 M, we have screened a panel of IgM-producing hybridomas prepared from peritoneal cells enriched in B-

213 T cell hybridomas produced from these joint-derived cells revea

214 BV-transformed B-CLL cells, and these hetero-hybridomas produced immunoglobulin.

215 The majority (92%) of CD8(+) T cell hybridomas produced large amounts of IFN-gamma only when

216 under these conditions, most rescued B cell hybridomas produced mAbs that lacked HIV-1 Envelope (Env

217 y and binding affinity to Stx2 as the parent hybridoma-produced 5C12.

218 tumor mass in a syngeneic host compared to a hybridoma producing a monoclonal antibody to the high mo

219 variable region genes isolated from a B cell hybridoma producing alphaGal-specific IgM Ab that make i

220 We also showed that a hybridoma producing monoclonal antibody C11C1 injected i

221 monoclonal antibody line RS6, selected from hybridomas raised against sieve elements isolated from C

222 We generated a set of hybridomas reactive with filarial E/S products and scree

223 Using a hybridoma sampling approach, we found that SNF1 T cells

224 nd mouse tumor rectal (RIT) cell lines using hybridoma-screened Dulbecco's modified Eagle's medium an

225 The conventional hybridoma screening and subcloning process is generally

226 In this study, mice implanted with hybridoma secreting 6-19 IgA anti-IgG2a rheumatoid facto

227 A subcutaneous hybridoma secreting IgE antibodies developed.

228 Generation of hybridomas secreting human mAbs has been previously repo

229 y human hybridoma technology, we isolated 37 hybridomas secreting human MAbs to dengue viruses from 1

230 From hybridomas secreting IgG Abs reactive with apoptotic cel

231 neration, enrichment, and screening of mouse hybridomas secreting mAb specific for a protein of inter

232 Three hybridomas secreting mAbs with anti-LukAB activity (desi

233 the epitopes recognized by Dob1, which is a hybridoma-secreting human immunoglobulin G2 antibody to

234 dNKT hybridomas showed distinct reactivities for diverse anti

235 mice present exogenous protein Ags to T cell hybridomas similarly well, but H2-O(-/-) DCs induce stro

236 cted macrophages were overlaid with a T cell hybridoma specific for an Ag85B epitope complexed with M

237 tigen-loaded FLS were cocultured with T cell hybridomas specific for immunodominant portions of human

238 ity complex-deficient background, and T cell hybridomas specific for the glycosylated and nonglycosyl

239 e points from 10 min to 4 h, in a 1B6 T cell hybridoma stimulated by a set of three myelin proteolipi

240 gene segment use was diverse even among IgG hybridomas suggesting that the V(L) is less critical to

241 hared multiple R mutations with anti-DSG1 FS hybridomas, suggesting selection by the same or a simila

242 PG, lipids from both sources stimulated dNKT hybridomas, suggesting that presentation of microbial li

243 ; these reporters can detect specific mAb in hybridoma supernatants via plate-bound Ab-capture arrays

244 in proteolipid altered peptide ligands and a hybridoma T cell line derived from a mouse model of expe

245 nii Five anti-OmpA MAbs were developed using hybridoma technology and showed strong binding to strain

246 active human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of health

247 coprotein precursor complex (GPC) and murine hybridoma technology to generate 25 mouse monoclonal ant

248 We utilized hybridoma technology to produce fully human immunoglobul

249 A new hybridoma technology using human B cells from house dust

250 Hybridoma technology was the breakthrough that enabled t

251 Using a high-efficiency human hybridoma technology, we isolated 37 hybridomas secretin

252 Using hybridoma technology, we isolated a panel of H10- and N8

253 tics from murine mAbs produced from standard hybridoma technology.

254 bs against ETN (MA-ETN) were generated using hybridoma technology.

255 myeloma fusion partner allowed us to recover hybridomas that captured naturally primed, antigen-speci

256 from 2C-TCR transgenic mice and from T cell hybridomas that expressed the 2C TCR or a high-affinity

257 zed by surface plasmon resonance, and T cell hybridomas that expressed these TCR, with or without CD8

258 monoclonal antibody (MAb) from a library of hybridomas that inhibited the binding of T. vaginalis or

259 sonance (SPR) binding assay, we selected six hybridomas that produced mAbs with 10(-11) M binding aff

260 e mice yielded splenic B cells for preparing hybridomas that secrete chimeric human IgE specific for

261 To study the etiology of FS, hybridomas that secrete either IgM or IgG (predominantly

262 tion assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galact

263 alysis in mouse primary T cells and a T cell hybridoma to define the regulatory enhancers responsible

264 We have developed an IgE hybridoma to LABD97 antigen.

265 Intra-abdominal injection of 2B11.3 hybridoma to NEP25 mice (NEP25/hybridoma) caused IC depo

266 An IgE mouse hybridoma to trinitrophenyl was used as a control.

267 es how to use microengraving to screen mouse hybridomas to establish new cell lines producing unique

268 Finally, we engineer mouse hybridomas to secrete Fab' fragments instead of the whol

269 the intestines of mice bearing subcutaneous hybridoma tumors.

270 were well expressed on primary T cells or T hybridomas using a tricistronic (alpha, beta, green fluo

271 This hybridoma was injected subcutaneously in SCID mice with

272 -39 peptide, and a set of peptide-responsive hybridomas was derived.

273 resolution, a panel of P1 HA-specific B cell hybridomas was generated following immunization of mice

274 n GAD (hGAD65)-specific CD4 T-cell lines and hybridomas was generated to serve as detection reagents

275 he antibodies secreted from the HCV-infected hybridomas was impaired.

276 Comparing different T cell hybridomas, we identified key residues on the MHCII alph

277 - or lenti-virus to IgM(+) mouse B cells and hybridomas, we induce class-switch recombination (CSR) o

278 Using this assay on engineered T-cell hybridomas, we observed approximately 85% accurate pairi

279 ybridoma, and a nonprotective IgG1-producing hybridoma were compared.

280 Twenty-six stable hybridomas were derived from BALB/c mice immunized with

281 Additionally, the IgG hybridomas were extensively mutated and the distribution

282 Seven hybridomas were found to cross-react with NF-M15-35.

283 the etiology of this disease, CD4(+) T cell hybridomas were generated from inflamed tissue-derived C

284 rigger their proliferation, V beta 4+ T cell hybridomas were generated from the lungs and spleens of

285 T cell hybridomas were generated to investigate cross-reactivit

286 boost with whole virus, antibody-expressing hybridomas were generated.

287 Hybridomas were produced and clones selected for their r

288 Hybridomas were produced by fusing splenocytes with mous

289 A majority of T cell hybridomas were specific for myelin protein 0 (P0), whic

290 Hybridomas were stained with the DR4-gp-39 tetramer and

291 any surface molecule on the cell lines, the hybridomas were stimulated most frequently by pMHC ligan

292 Human hybridomas were then generated and characterized.

293 ma (P815) cells, supported IL-2 secretion of hybridomas when substituted for syngeneic splenocytes as

294 omas is diverse, but is restricted among IgG hybridomas, where the majority uses one of two V(H) gene

295 e deaminase levels were greatest in the B6.1 hybridomas, which may explain the instability.

296 the source of B cells for the preparation of hybridomas, which secreted monoclonal human IgE antibodi

297 We isolated hybridomas with anti-GA specificity from MRL/lpr mouse k

298 by interrogating a panel of dNKT mouse cell hybridomas with lipid extracts from the pathogen Listeri

299 ation frequency than those in the uninfected hybridomas, with mutations concentrating in complementar

300 ase (Lck)-associated CD4 molecules in T-cell hybridomas would allow for the detection of subthreshold