ライフサイエンスコーパス: hydratase

1 quence that spans four exons of the fumarate hydratase.

2 ially larger degree of covalency for nitrile hydratase.

3 y redox behavior at the iron site in nitrile hydratase.

4 rtant to the catalytic activity of enoyl-CoA hydratase.

5 in mitofusin1, peroxiredoxin 4, and fumarate hydratase.

6 required for the high kcat of the enoyl-CoA hydratase.

7 tified as the catalytic residue of enoyl-CoA hydratase.

8 t encodes for the TCA cycle enzyme, fumarate hydratase.

9 ntaining protein and mitochondrial aconitate hydratase.

10 , of the Mycobacterium tuberculosis fumarate hydratase.

11 te determination of the activity of fumarate hydratase.

12 and RpfF shows some relatedness to enoyl CoA hydratases.

13 gy, respectively, to other sequenced nitrile hydratases.

14 that exceeding nutrients suppress Enoyl-CoA hydratase-1 (ECHS1) activity by inducing its acetylation

15 Herein, we report that enoyl-CoA hydratase-1 (ECHS1), the enzyme involved in the oxidatio

16 Aconitase-2 and enoyl-CoA-hydratase-1 expression levels were decreased in L-NMMA-t

17 Depletion of aconitase-2 and enoyl-CoA-hydratase-1 resulted in the inhibition of the Krebs cycl

18 aldehyde dehydrogenase-1; D204 in enoyl CoA hydratase-1), as well as residues of unknown function (e

19 depletion of both aconitase-2 and enoyl-CoA-hydratase-1.

20 ng protein 1), and Ehhadh (enoyl-coenzyme A, hydratase/3-hydroxyacyl coenzyme A dehydrogenase)], and

21 ntribute to renal function include enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EHHADH) and a

22 es: fatty acyl-CoA oxidase (ACOX), enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD), and thio

23 l-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD), peroxiso

24 noate hydrolase, 2-hydroxypenta-2,4-dienoate hydratase, 4-hydroxy-2-oxovalerate aldolase, and acetald

25 Fumarate hydratase, a component of the tricarboxylic acid cycle,

26 the iron(III) site of Fe-containing nitrile hydratase, a designed ligand PyPSH(4) with two carboxami

27 2 (formerly Brevibacterium sp. R312) nitrile hydratase, a novel non-heme iron enzyme, have a large nu

28 Importantly, loss of function of fumarate hydratase, a tumor suppressor and TCA cycle component, c

29 and acetamiprid) and transcripts of nitrile hydratases, a class of enzymes that we experimentally co

30 tion of the nitrile substrate to the nitrile hydratase active site low spin Fe(3+) center.

31 ng with mechanisms for the decarboxylase and hydratase activities.

32 ing additional biosynthetic routes including hydratase activity (e.g., CLA-HY, FA-HY1, FA-HY2).

33 ultiple proteins in the mantle with alpha-CA hydratase activity and mapped to a protein with a simila

34 The results provide evidence for a hydratase activity and set the stage to use the 3-halopr

35 on to the decarboxylase activity, MSAD has a hydratase activity as demonstrated by the MSAD-catalyzed

36 E111Q4-OD/VPH complexes, which retained full hydratase activity but had little decarboxylase activity

37 led to a 28-fold reduction in 2-butenoyl-CoA hydratase activity in a preparation of organelles.

38 results provide additional evidence for the hydratase activity of MSAD and further support for the h

39 and Arg-75 have also been implicated in the hydratase activity of MSAD in which 2-oxo-3-pentynoate i

40 a mechanism-based inhibitor activated by the hydratase activity of MSAD, have been determined.

41 singly, ECH exhibiting less than 2% residual hydratase activity retains essentially 100% beta-elimina

42 nd in Bacillus subtilis, exhibit a low level hydratase activity that converts trans-3-haloacrylates t

43 A weak enoyl-CoA hydratase activity was detected for both DpgB and DpgD.

44 sing" homologue of Glu144 fails to introduce hydratase activity with the substrate analogues.

45 A dehydrogenase also has intrinsic enoyl-CoA hydratase activity, a property of other members of the a

46 exhibit beta-methylcrotonyl-coenzyme A (CoA) hydratase activity, as we predicted.

47 H-CoA effects rapid and irreversible loss of hydratase activity.

48 ype complex, but is devoid of any detectable hydratase activity.

49 and Rv2499c and Rv3389c possess itaconyl-CoA hydratase activity.

50 y with a trans-o-hydroxybenzylidene-pyruvate hydratase-aldolase gene (pbhC).

51 from pharmacological inhibition of fumarate hydratase (also known as fumarase).

52 ene encoding the Krebs cycle enzyme fumarate hydratase, an early shift to aerobic glycolysis, and rap

53 transcripts, that this gene encodes fumarate hydratase, an enzyme of the tricarboxylic acid cycle.

54 been obtained for HD-CoA bound to enoyl-CoA hydratase, an enzyme system that has also previously bee

55 Fumarate hydratase, an essential enzyme in the tricarboxylic acid

56 genic variants in the gene encoding fumarate hydratase and an increased risk of papillary renal-cell

57 In performing both hydratase and dehydratase activities, Nmar_1308 reduces

58 ultifunctional enzyme that minimally encodes hydratase and dehydrogenase activities.

59 their sequence, OdaA and OdaI have predicted hydratase and dioxygenase reductase activities, respecti

60 another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belonging to the

61 These results imply that loss of fumarate hydratase and fumarate accumulation contribute to the ag

62 l-CoA (HD-CoA), bound to wild-type enoyl-CoA hydratase and G141P, a mutant in which a hydrogen bond t

63 en bound as an enolate to MCAD and enoyl-CoA hydratase and is used to rationalize the observation tha

64 nts predominantly map to genes for aconitate hydratase and isocitrate dehydrogenase, which are expect

65 e reactions, two of which (2-trans enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenase) are cor

66 the active site of long-chain 2,3-enoyl-CoA hydratase and long-chain 3-ketoacyl-CoA thiolase.

67 DNA-encoded mitochondrial genes for fumarate hydratase and succinate dehydrogenase have been linked t

68 des a linker domain between the NH2-terminal hydratase and the COOH-terminal 3-hydroxyacyl-CoA dehydr

69 Here we show that loss of fumarate hydratase and the subsequent accumulation of fumarate in

70 Shy (side chain hydratase) and Sal (side chain aldolase), are involved i

71 A hydrolase, cyclohexa-1,5-dienecarbonyl-CoA hydratase, and a fluoride-proton antiporter gene, that m

72 erties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-trans-enoyl-CoA isome

73 l carcinoma cells with mutations in fumarate hydratase, and in cells with normal mitochondria subject

74 as NADH peroxidase and NADH oxidase, nitrile hydratase, and the hORF6 and AhpC peroxiredoxins.

75 the metal ion in the iron-containing nitrile hydratases are conserved in this enzyme, suggesting that

76 Nitrile hydratases are enzymes involved in the conversion of nit

77 ristic curve (AUC) for cytoplasmic aconitate hydratase, argininosuccinate synthase, carbamoylphosphat

78 finity labeling strategy identified fumarate hydratase as the principal pharmacological target.

79 a significant change in the Km value of the hydratase as well as a 5.9- and 62-fold increase, respec

80 f the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives t

81 ichia coli enzyme 2-hydroxypentadienoic acid hydratase assembles to form a 20-nm-diameter particle co

82 yl group by 3-vinyl bacteriochlorophyllide a hydratase (BchF) followed by 3-hydroxyethyl bacteriochlo

83 phosphoenolpyruvate carboxykinase, aconitate hydratase, branched-chain alpha-keto acid dehydrogenase

84 ted that Ltp2 forms a tight complex with the hydratase but that each enzyme retains an independent Co

85 A divergent fumarate hydratase C (fumC) gene lies further upstream.

86 protic residues is directly involved in the hydratase catalysis, the multienzyme complexes with eith

87 pids containing FAHFAs via sequential steps: hydratase-catalyzed hydration of unsaturated fatty acids

88 Enoyl-CoA hydratase catalyzes the hydration of trans-2-crotonyl-Co

89 the tricarboxylic acid cycle enzyme fumarate hydratase cause hereditary leiomyomatosis and renal cell

90 Significantly, binding to enoyl-CoA hydratase causes the chemical shifts of the C1 and C3 HD

91 HB cycle, functioning as both a crotonyl-CoA hydratase (CCAH) and 3-hydroxypropionyl-CoA dehydratase

92 main derived from the C-terminal domain of a hydratase (ChsH2(DUF35)) that catalyzes the preceding st

93 d a plant resolve in the prokaryotic nitrile hydratase clade.

94 the active site of cobalt containing nitrile hydratase (Co NHase) was prepared.

95 of the crotonase family including enoyl-CoA hydratase (crotonase) and methylmalonyl-CoA decarboxylas

96 Members of the enoyl-CoA hydratase (crotonase) superfamily catalyze different ove

97 r genes encoded homologs of a 1,2-carotenoid hydratase (CrtC), an O-methyltransferase (CrtF), and two

98 e desaturase (crtU/CT0323), carotenoid 1',2'-hydratase (crtC/CT0301), and carotenoid cis-trans isomer

99 forms a complex with C. testosteroni steroid hydratase (CtShy).

100 Many children with fumarate hydratase deficiency do not survive infancy or childhood

101 ittent porphyria; delta amino-levulinic acid hydratase deficiency porphyria; hereditary coproporphyri

102 so occur in the recessive condition fumarate hydratase deficiency, and some parents of people with th

103 legia, giant axonal neuropathy, and fumarate hydratase deficiency.

104 Fumarate hydratase-deficient (FH-deficient) renal cell carcinoma

105 cule metabolite that accumulates in fumarate hydratase-deficient cells, plays a key role in cell tran

106 Fumarate hydratase-deficient renal cancers are highly aggressive

107 ibute to the aggressive features of fumarate hydratase-deficient tumours.

108 tified a petunia gene encoding cinnamoyl-CoA hydratase-dehydrogenase (PhCHD), a bifunctional peroxiso

109 SD IV contains' a region homologous to yeast hydratase-dehydrogenase-epimerases and to sterol carrier

110 letion analyses confirmed that the enoyl-CoA hydratase/dehydrogenase Fox2p, the putative 3-hydroxypro

111 es show that kidney cancers lacking fumarate hydratase display increased sensitivity to agents that i

112 Enoyl-CoA hydratase domain-containing 3 (ECHDC3) was the top-ranke

113 Isocyanide (formerly isonitrile) hydratase (EC 4.2.1.103) is an enzyme of the DJ-1 superf

114 deficiency of the enzyme fumarase (fumarate hydratase, EC 4.2.1.2) which result in autosomal recessi

115 he crystal structure of the enzyme enoyl-CoA hydratase (ECH) from rat liver with the bound substrate

116 opyl)glycine, against bovine liver enoyl-CoA hydratase (ECH) were characterized.

117 an be directly bioactivated by the enoyl-CoA hydratase (ECH) with the release of 1,2-dichloro-3,3,3-t

118 chain fatty acyl-CoAs, employing 2-enoyl-CoA hydratase (ECH), 3-hydroxyl-CoA dehydrogenase (HAD), and

119 D 3q1t) has been reported to be an enoyl-CoA hydratase (ECH), but SALSA analysis shows a poor match b

120 rected inactivator of bovine liver enoyl-CoA hydratase (ECH).

121 h auxiliary enzymes, including the enoyl-CoA hydratase ECH2, convert (R)-3-hydroxyacyl-CoA intermedia

122 mologous to mammalian enoyl-coenzyme A (CoA) hydratases, EchA6 is non-catalytic yet essential and bin

123 rotonyl-CoA suggesting short chain enoyl-CoA hydratase (ECHS1) activity was inhibited.

124 ibited the activity of short chain enoyl-CoA hydratase (ECHS1).

125 CDH) with downregulation of the crotonyl-CoA hydratase enoyl-CoA hydratase short chain 1 (ECHS1), lea

126 the gene encoding the 2-methyl-cis-aconitate hydratase enzyme is encoded outside the prpBCDE operon.

127 coding the tricarboxylic acid cycle fumarate hydratase enzyme.

128 tion multi-catalytic system, merging nitrile hydratase enzymes with a Cu-catalysed N-arylation reacti

129 ferentially conserved between hydrolases and hydratases established that this position is relevant to

130 of the active site of Fe-containing nitrile hydratase (Fe-NHase), a model complex of the NO-bound ac

131 Surprisingly, however, fumarate hydratase (FH) activity was also upregulated in obese li

132 has extensive homology to the human fumarate hydratase (FH) and encodes a 288-amino acid protein (Mw

133 enes succinate dehydrogenase (SDH), fumarate hydratase (FH) and isocitrate dehydrogenase (IDH), advan

134 of Plasmodium falciparum, with only fumarate hydratase (FH) and malate-quinone oxidoreductase (MQO) b

135 we targeted two TCA cycle enzymes, fumarate hydratase (FH) and succinate dehydrogenase (SDH), and co

136 uclear-encoded Krebs cycle enzymes, fumarate hydratase (FH) and succinate dehydrogenase (SDHB, -C and

137 genes, or germline mutations in the fumarate hydratase (FH) and succinate dehydrogenase genes (SDHA,

138 icarboxylic acid (TCA) cycle enzyme fumarate hydratase (FH) are associated with a highly malignant fo

139 Succinate dehydrogenase (SDH) and fumarate hydratase (FH) are components of the tricarboxylic acid

140 t succinate dehydrogenase (SDH) and fumarate hydratase (FH) are tumour suppressors and which associat

141 the tricarboxylic acid cycle enzyme fumarate hydratase (FH) associates with a malignant form of renal

142 Mutation of fumarate hydratase (FH) at 1q43 is known to cause the Mendelian s

143 icarboxylic acid (TCA) cycle enzyme fumarate hydratase (FH) cause a hereditary cancer syndrome known

144 Mutations in fumarate hydratase (FH) cause hereditary leiomyomatosis and renal

145 the tricarboxylic acid cycle enzyme fumarate hydratase (FH) causes hyperaccumulation of fumarate.

146 Pathogenic mutations in fumarate hydratase (FH) drive hereditary leiomyomatosis and renal

147 uccinate dehydrogenase A (SDHA) and fumarate hydratase (FH) expression.

148 the tricarboxylic acid cycle enzyme fumarate hydratase (FH) further increases intracellular fumarate

149 cation of germline mutations in the fumarate hydratase (FH) gene in European families supports it as

150 tivating pathogenic variants in the fumarate hydratase (FH) gene, which encodes the enzyme that catal

151 The tricarboxylic acid cycle enzyme fumarate hydratase (FH) has been identified as a tumor suppressor

152 the tricarboxylic acid cycle enzyme fumarate hydratase (FH) have been linked to an aggressive variant

153 intracellular fumarate, a result of fumarate hydratase (FH) inactivation, but it is not clear how NRF

154 Fumarate hydratase (FH) is a mitochondrial enzyme that catalyzes

155 Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid cy

156 Patients with germline fumarate hydratase (FH) mutation are predisposed to develop aggre

157 ene encoding the Krebs cycle enzyme fumarate hydratase (FH) predispose to hereditary leiomyomatosis a

158 succinate dehydrogenase (SDH), and fumarate hydratase (FH) that produce oncometabolites that competi

159 infection, is regulated by the host fumarate hydratase (FH) to limit inflammation.

160 that regions at or around CpDAAs in fumarate hydratase (FH) were enriched for VUSs and pathogenic var

161 Fumarate hydratase (FH), a key node of mitochondrial metabolism,

162 utations, biallelic inactivation of fumarate hydratase (FH), and collagen, type IV, alpha 5 and colla

163 Expression of fumarate hydratase (FH), which regulates urine fumarate accumulat

164 type of kidney cancer that harbors fumarate hydratase (FH)-inactivating mutations and has elevated R

165 the tricarboxylic acid cycle enzyme fumarate hydratase (FH).

166 succinate dehydrogenase (SDH), and fumarate hydratase (FH).

167 l and mutation of the gene encoding fumarate hydratase (FH).

168 Germline mutations of the fumarate hydratase (FH, fumarase) gene are found in the recessive

169 Class-II fumarases (fumarate hydratase, FH) are dual-targeted enzymes occurring in th

170 study, we investigated the role of fumarate hydratase (Fh1), a key component of the mitochondrial tr

171 and that RS can distinguish between Fumarate hydratase (Fh1)-deficient and Fh1-proficient cells based

172 Fumarate hydratases (FHs) are essential metabolic enzymes grouped

173 Monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli has been purified 3800-f

174 r crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofol

175 r crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofol

176 cond and third shells of the Co-type nitrile hydratase from Pseudomonas putida (ppNHase) that may be

177 Nitrile hydratase from Pseudomonas putida NRRL-18668 has been pu

178 data were obtained for the iron-type nitrile hydratase from Rhodococcus equi TG328-2 (ReNHase) using

179 The alphabeta dimer of active nitrile hydratase from Rhodococcus sp. R312 contains one low-spi

180 The absence of nitrile hydratase from several sequenced species indicates that

181 The fungal-specific enzyme homoaconitate hydratase from this pathway is moderately similar to the

182 Mycobacterium tuberculosis (Mtb) fumarate hydratase (fumarase) is a highly conserved essential pro

183 Fumarate hydratase (fumarase), a vulnerable component of the citr

184 requires a 4-carboxy-2-hydroxymuconate (CHM) hydratase (GalB), which has a 12% sequence identity to a

185 Germline mutations in the fumarate hydratase gene (FH) predispose to multiple cutaneous and

186 The nitrile hydratase gene in M. brevicollis was believed to have ar

187 nce presented here demonstrates that nitrile hydratase genes are present in multiple eukaryotic super

188 NGS: Here we report the detection of nitrile hydratase genes in five eukaryotic supergroups: opisthok

189 e specific acyl-CoA synthetase and enoyl-CoA hydratase genes.

190 ATP-synthase delta chain and Enoyl-CoenzymeA hydratase, glutathione-s-transferase omega, alpha-1-acid

191 yme A (HD-CoA) bound to the enzyme enoyl-CoA hydratase has been determined using transferred nuclear

192 64 and E144) in the active site of enoyl-CoA hydratase has been probed by site-directed mutagenesis.

193 9 and 9.2, whereas the Glu119 --> Gln mutant hydratase has only a single pKa of 9.5.

194 of Shy provides insight into how MaoC family hydratases have adapted to accommodate large polycyclic

195 they are widespread in prokaryotes, nitrile hydratases have only been reported in two eukaryotes: th

196 of the PPARalpha target genes rat enoyl-CoA hydratase (HD) and peroxisomal fatty acyl-CoA oxidase (A

197 derstand the structural basis for isocyanide hydratase (ICH) catalysis, we determined the crystal str

198 how covalent catalysis modulates isocyanide hydratase (ICH) conformational dynamics throughout its c

199 ve catalysis in a designed mutant isocyanide hydratase (ICH) enzyme that enhances sampling of importa

200 2 (formerly Brevibacterium sp. R312) nitrile hydratase in frozen solutions at pH 7 and 9 has been ana

201 The presence of nitrile hydratases in many other eukaryotic groups is unresolved

202 OHEDH is distinct from other hydratases in that it requires Mn(2+) as a cofactor.

203 s recover a clade of eukaryotic-type nitrile hydratases in the Opisthokonta, Amoebozoa, SAR and CCTH;

204 family of nonheme iron enzymes, the nitrile hydratases, in which post-translational oxidation of two

205 NO-sensitive [4Fe-4S] (de)hydratases, including the Krebs cycle aconitase and the

206 egulation of ech-1.1 (a homolog of enoyl-CoA hydratase involved in fatty acid beta-oxidation) and tub

207 These data suggest that enoyl-CoA hydratase is an important enzyme in the bioactivation of

208 Loss of fumarate hydratase is associated with suppression of miR-200 and

209 2-Hydroxypentadienoic acid hydratase is found on many bacterial catabolic pathways

210 Activity of fumarate hydratase is reduced in lymphoblastoid cells from indivi

211 showed significant homology to the enoyl-CoA hydratase/isomerase enzyme family.

212 sociated with the members of the 2-enoyl-CoA hydratase/isomerase enzyme superfamily are compared to s

213 active site of one member of the 2-enoyl-CoA hydratase/isomerase family, 4-chlorobenzoyl-CoA dehaloge

214 moderate sequence identity to members of the hydratase/isomerase superfamily of enzymes.

215 arity to FabA, but rather is a member of the hydratase/isomerase superfamily.

216 ication of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of th

217 se family and that IBR10 resembles enoyl-CoA hydratases/isomerases.

218 a C-terminal module homologous to enoyl-CoA hydratases/isomerases.

219 at the level of the second enzyme, enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE) of t

220 In this study, the function of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE), the

221 d transcription from a peroxisomal enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase bifunctional

222 y of CBP and TRAP150, to the mouse enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase gene promote

223 strates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed.

224 ence identity to a previously identified CHM hydratase (LigJ) fromSphingomonassp. SYK-6.

225 in this enzyme, suggesting that this nitrile hydratase, like the enzyme from Rhodococcus rhodochrous

226 owever, atypical RiPP leaders called nitrile hydratase-like leader peptides (NHLPs) are longer and st

227 mponent enzymes of TOC, long-chain enoyl-CoA hydratase, long-chain 3-hydroxyacyl-CoA dehydrogenase, a

228 wn that triosephosphate isomerase, aconitate hydratase, M-protein, nucleoside diphosphate kinase B, a

229 -oxidation mutants showed that the enoyl CoA-hydratase MAOC-1 serves an important role in ascaroside

230 (e.g., isocitrate dehydrogenase and fumarate hydratase) may enhance or mimic the effects of recurrent

231 rt a previously unassigned modular enoyl-CoA hydratase (mECH) domain and the assembly of enzyme const

232 Nitrile hydratase metalloenzymes are unique and important biocat

233 enzyme assigned as (3S)-methylglutaconyl-CoA hydratase (MGCH), which catalyzes the syn-hydration of (

234 In contrast, growth of a putative enoyl-CoA hydratase mutant (DeltaechA) was abolished on short-chai

235 vels in succinate dehydrogenase and fumarate hydratase-mutant tumors, were identified as potent Tet i

236 ing on nitrile metabolizing enzymes: nitrile hydratase (NHase) and amidase versus nitrilase activity.

237 Nitrile hydratase (NHase) catalyzes the hydration of nitriles to

238 Nitrile hydratase (NHase) is an iron-containing metalloenzyme th

239 Nitrile hydratase (NHase) is one of a growing number of enzymes

240 ive site of the non-heme iron enzyme nitrile hydratase (NHase) is studied using sulfur K-edge XAS and

241 catalytic role in the metalloenzyme nitrile hydratase (NHase), a reactive five-coordinate Co(III) th

242 o the catalytic mechanism of Fe-type nitrile hydratases (NHase), the pH and temperature dependence of

243 oth cysteine dioxygenases (CDOs) and nitrile hydratases (NHases), and yet the mechanisms by which sul

244 e a detailed catalytic mechanism for nitrile hydratases (NHases), the pH and temperature dependence o

245 DI) and two-oxo-hept-3-ene-1,7-dioate (OHED) hydratase (OHEDH).

246 nd related lactobacilli, including an oleate hydratase (ohyA) and putative fatty acid efflux pump (fa

247 The soluble flavoprotein oleate hydratase (OhyA) hydrates the 9-cis double bond of unsat

248 Oleate hydratases (OhyAs) belong to a large family of bacterial

249 inucleotide (FAD)-dependent bacterial oleate hydratases (OhyAs) catalyze the addition of water to iso

250 rboxylase (gamma-RSD), and 4-oxalomesaconate hydratase (OMAH).

251 ism caused by deficient activity of fumarate hydratase, one of the constituent enzymes of the Krebs t

252 riments show that the MFP2 2-trans enoyl-CoA hydratase only exhibits activity against long chain (C18

253 s encoding succinate dehydrogenase, fumarate hydratase or isocitrate dehydrogenase, can dysregulate s

254 on of the resonance Raman spectra of nitrile hydratase prepared at pH 7.3 and 9.0 shows a shift of in

255 The novel family of eukaryotic nitrile hydratases presented in this paper represents a promisin

256 ydratase (probably PrpD), 2-methylisocitrate hydratase (probably PrpD), and 2-methylisocitrate lyase

257 recapitulated by the incubation of fumarate hydratase-proficient cells with cell-permeable fumarate.

258 fer effect on the mechanism of the enoyl-CoA hydratase reaction is discussed.

259 quantitatively assessed from the K(m) in the hydratase reaction, 3 microM, and the K(i), 1.0 microM,

260 , is inactive in the dehydrogenation and the hydratase reactions.

261 ression of the gene ech-6 encoding enoyl-CoA hydratase remitted fat diet-induced deleterious effects

262 xA (Rv1470), trxB (Rv1471), and an enoyl-coA hydratase (Rv1472), indicating a possible role for CtpD

263 Two nitrile hydratase sequences from an animal and a plant resolve i

264 tion of the crotonyl-CoA hydratase enoyl-CoA hydratase short chain 1 (ECHS1), leading to accumulation

265 Furthermore, the wild-type hydratase shows a bell-shaped pH dependence of the kcat/

266 , the acyl-CoA ligase SidI and the enoyl-CoA hydratase SidH, linking biosynthesis of mevalonate and T

267 ed to 3-hydroxypropionyl-CoA by acryloyl-CoA hydratase (SPO0147).

268 logy validates the modeling of the enoyl-CoA hydratase structure with the 4-(chlorobenzoyl)-CoA dehal

269 onal protein deficiency, due to mutations in hydratase subunit A (HADHA), results in sudden infant de

270 e, and is a fusion of beta and alpha nitrile hydratase subunits.

271 ncer, including the VHL, MET, FLCN, fumarate hydratase, succinate dehydrogenase, TSC1, TSC2, and TFE3

272 art of a consensus sequence in the enoyl-CoA hydratase superfamily, the results presented here provid

273 seven paralogues of the crotonase (enoyl CoA hydratase) superfamily.

274 th the strong sequence homology to enoyl-CoA hydratase support the intramolecular suprafacial transfe

275 s enzyme is a member of the class of nitrile hydratase that contains cobalt.

276 s, including a missense mutation in fumarate hydratase that controls variation in the mitochondrial u

277 t from previously characterized MaoC steroid hydratases that are (pseudo) heterodimers with one subst

278 In nitrile hydratase, the active-site Cys-SOH functions in both iro

279 Like the other cobalt-containing nitrile hydratases, this enzyme is relatively stable, maintainin

280 ompounds where ring-cleavage is achieved via hydratases, this lyase might represent a new ring-openin

281 Our assay uses fumarate hydratase to convert fumarate to malate and uses oxaloac

282 r at the active site of the enzyme acetylene hydratase to facilitate acetylene hydration to acetaldeh

283 We have used the similarity of homoaconitate hydratase to isopropylmalate isomerase (serving in leuci

284 ed glutamate residues of rat liver enoyl-CoA hydratase to which Glu119 and Glu139 of the large alpha-

285 onate decarboxylase (4-OD) and vinylpyruvate hydratase (VPH) from Pseudomonas putida mt-2 form a comp

286 otonate tautomerase (4-OT) and vinylpyruvate hydratase (VPH) from the catechol meta-fission pathway a

287 alytically essential in homologous enoyl-CoA hydratases was also essential in CHY1.

288 a coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome,

289 rmore, we identify an Mtb-encoded isonitrile hydratase, which can possibly mediate intracellular zinc

290 nzyme activity of an iron-containing nitrile hydratase, which requires a catalytic alphaCys114-SOH in

291 vity changes caused by mutations in fumarate hydratase, which underlie the familial cancer predisposi

292 udes ACMSD and ACMSD-like decarboxylases and hydratases with diverse substrate specificities, many of

293 ease, respectively, in the kcat of enoyl-CoA hydratase without a significant change in the Km value o

294 y an acyl-CoA dehydrogenase and a subsequent hydratase yielding an intermediate with a tertiary hydro