ライフサイエンスコーパス: immobilized protein

1 l as charged amino acids associated with the immobilized protein.

2 developed using magnetic beads containing an immobilized protein.

3 robing the homogeneity of the populations of immobilized protein.

4 arfarin is small compared to the size of the immobilized protein.

5 on, allowing the detection down to 2 nmol of immobilized protein.

6 he visible range around 425nm, caused by the immobilized proteins.

7 robust single-molecule detection of surface-immobilized proteins.

8 flowed through the capillary to bind to the immobilized proteins.

9 can influence the binding activities of the immobilized proteins.

10 gel matrix; and (v) in-gel immunoprobing of immobilized proteins.

11 anic complexes shuttle electrons between the immobilized proteins.

12 nstant (Ka) for human IgG binding to surface-immobilized protein A is determined to be (3.0 +/- 0.5)

13 ques measuring biotinylated gC1qR binding to immobilized protein A revealed specific binding that was

14 o that in NHS preabsorbed by the yeast or by immobilized protein A/G to remove serum immunoglobulin.

15 In addition, immobilized protein activity levels were high, typically

16 containing DNA conjugated oligos to identify immobilized protein analysts using real-time qPCR.

17 of the formation of a bound complex between immobilized protein and immobilized ligand as a function

18 correlation was found between the amount of immobilized protein and the amplitude of the pH transiti

19 ays are based on the interaction between the immobilized protein and the sampled analyte directly on

20 The activity of the immobilized protein and the surface density were determi

21 ecifically designed for staining NC membrane-immobilized proteins and is faster and more sensitive th

22 on of resolved proteins, antibody probing of immobilized proteins, and all interim wash steps.

23 s used for investigating the conformation of immobilized proteins, and then examines how protein load

24 Because no stripping is involved, immobilized proteins are not lost from the membrane, thu

25 This research describes how to prepare immobilized protein arrays and how to assay the binding

26 RNA pull-down and immobilized protein assays showed that hrp48 protein bin

27 scaffold for subsequent antibody probing of immobilized protein bands.

28 sed on analysis of protein conformation, the immobilized proteins bind with partner antibody after SD

29 Moreover, using an immobilized protein-binding assay, we found that phospho

30 ndent fashion, Kd (app) = 29 nM and bound to immobilized protein C in a Ca2+-dependent fashion.

31 of a complex between immobilized ligand and immobilized protein close to that for the formation of c

32 iles has been developed, based on the use of immobilized protein columns and HPLC.

33 st the epsilon subunit was passed over these immobilized protein columns, and the purified antibodies

34 residual activity of 40% for the covalently immobilized protein compared to 20% of residual activity

35 igated the binding of fluid-phase CRP to six immobilized proteins: complement factor H, oxidized low-

36 double resonance (ELDOR) spectroscopy on an immobilized protein containing RX all reveal a highly co

37 In this experiment, the immobilized protein content and LDH activity on each mod

38 t 3 logs was observed for protein stains and immobilized protein content, with a lower limit of detec

39 obilized proteins, it was necessary that the immobilized proteins, except factor H, were also exposed

40 h immobilized proteins, indicating that some immobilized proteins express an Abeta epitope.

41 of high-capacity silica supports containing immobilized protein G.

42 C4BP bound to immobilized protein H or AP1 bacteria retained its cofac

43 Arrays of immobilized proteins have been developed for the discove

44 the buffer pK(a) value(s), and the amount of immobilized protein.Hence, it can be used to design pH t

45 dividual protein kinases on a complex mix of immobilized proteins, (ii). the fractionation of the pho

46 ely for isolating aptamers against a surface-immobilized protein (immunoglobulin E) and a solution-ph

47 d thioredoxin showed the presentation of the immobilized protein in a sterically accessible orientati

48 americ H2O2-treated CRP bound to a number of immobilized proteins including oxidized LDL, IgG, amyloi

49 yloid-beta peptide 1-42 (Abeta) reacted with immobilized proteins, indicating that some immobilized p

50 d support, and because the orientation of an immobilized protein is important for its function.

51 Understanding the properties of immobilized proteins is critical to the optimal design o

52 ase interactions between small molecules and immobilized proteins is of intense interest, especially

53 fficient binding of acidic pH-treated CRP to immobilized proteins, it was necessary that the immobili

54 s developed, in which both the deposition of immobilized protein layers and the electrophoretic deliv

55 e quantify relates linearly to the number of immobilized protein molecules (R(2) = 0.98), and our pre

56 Immobilized protein molecules on the surface of an alumi

57 us, our procedure allows for single, surface-immobilized protein molecules to be detected with high s

58 rd compact disc (CD) as a biochip containing immobilized protein molecules.

59 of multistep reaction procedures leading to immobilized proteins on solid surfaces.

60 ction screening against a dilution series of immobilized proteins on the microarray enabling the obse

61 teins to acidic pH alter the conformation of immobilized proteins, our findings suggest that conforma

62 proach resulted in random orientation of the immobilized proteins over the surface.

63 microscopy of fluorescent ligand binding to immobilized protein partners.

64 on of low molecular weight ligand binding to immobilized protein receptors.

65 The immobilized proteins remain bioactive, as evidenced by e

66 ates; however, the identity of the ligand on immobilized proteins remains unknown.

67 gions and conditions studied for the surface immobilized proteins showed restricted motion, with indi

68 faces across space and provide insights into immobilized protein structure investigations for areas s

69 l inhibitory factor (NIF), C3bi, and certain immobilized protein substrates, represented by denatured

70 superficially inserted state; and 3), fully immobilized proteins, suggesting a fully inserted state.

71 l, we observe 50% immunoassay signal loss of immobilized protein targets during stripping rounds.

72 ing between small molecules and a variety of immobilized protein targets, pairs that have binding aff

73 estricted due to their strong binding to the immobilized protein targets.

74 importance given to determine the amount of immobilized protein, there is no simple method that allo

75 " packed with the same resin but without the immobilized protein to evaluate the dead volume, but thi

76 roteins on microtiter plates and exposure of immobilized proteins to acidic pH alter the conformation

77 Subsequent incubation of the immobilized protein using a fluorescently labeled or PEG

78 ), which overcomes this challenge by probing immobilized proteins using orthogonally labeled detectio

79 on of substantial conformational changes for immobilized proteins using SPR has been reported.

80 scence from single molecules attached to the immobilized proteins was detected with a high signal/noi

81 different CRP mutants capable of binding to immobilized proteins were constructed, and their binding

82 rase and lysozyme--the highest quantities of immobilized proteins were obtained when using a low ioni

83 e method utilized the ionic character of the immobilized protein while implementing biologically comp

84 and binding constants for the association of immobilized protein with free and immobilized ligand.