ライフサイエンスコーパス: importin

1 -3 proteins, CRM-1 (exportin-1 or XPO-1), or importins.

2 ) substantially decreased binding to several Importins.

3 stone tails are recognized by the individual Importins.

4 and forms an inverse gradient to cargo-bound importins.

5 on of Notch1 (N1IC) through interaction with importins.

6 BP via interactions with 14-3-3 proteins and importins.

7 ugh transient non-specific interactions with importins.

8 nzyme UBE2E3 and its nuclear import receptor importin 11 (Imp-11) regulate Nrf2 distribution and acti

9 In this study, we show that Importin-11 (Ipo11) is a transport receptor for PTEN tha

10 s in the matrix (MA) domain, is dependent on importin-11 and transportin-3 (TNPO3), which are known a

11 nt mice develop lung tumors, also implicates Importin-11 as a novel tumor suppressor.

12 unctions of importin-alpha/importin-beta and importin-11 have been verified in avian cells, whereas t

13 In this issue, Chen et al. show that Importin-11 traffics the tumor suppressor PTEN into the

14 IPO11 (Importin-11) is a nuclear import protein that shuttles c

15 DCD5 at Ser-119 to enhance its stability and importin 13-mediated nuclear translocation of PDCD5.

16 entally from that of the human Ubc9-importer Importin 13.

17 importin-beta, transportin-1, transportin-3, importin-13) or through adaptor proteins (e.g., importin

18 onclassical nuclear import pathway via IPO3 (importin 3, transportin 2) mediates stress-induced NEMO

19 We further disclosed that Importin 4 (IPO4) augmented the nuclear translocation of

20 of H3/H4 with the histone chaperone ASF1 and importin 4 is disrupted and the translocation of green f

21 We identified a novel role for importin-4 in governing the nuclear transport of HE4.

22 ermectin, an importin inhibitor, blocked HE4/importin-4 nuclear accumulation and sensitized HE4-overe

23 Here, we identified importin-5 (IPO5), a member of the importin family of nu

24 A myristoylated peptide that blocks importin 7-mediated ERK nuclear translocation induced ro

25 H2O2 plus IL-1beta had no direct effect on importin-7 but caused a significant loss (61.2+/-12.6% c

26 Knockdown of importin-7 by 38.4 +/- 11.5% (compared with control siRN

27 The importin-7 complex is essential for glucocorticoid funct

28 roxide (H2O2) plus IL-1beta costimulation on importin-7 expression, function, and glucocorticoid resp

29 We hypothesized that importin-7 is central to GR nuclear translocation and gl

30 snRNPs also fail to bind Ketel; however, the importin-7 orthologue Moleskin (Msk) physically associat

31 We investigated the effects of importin-7 siRNA on fluticasone propionate (FP)-induced

32 of Skp2, disruption of Skp2 interaction with importin-7, and decreased levels of p27/p57 in mouse len

33 of nuclear RanGTP, an essential cofactor for importin-7-mediated nuclear import of cargo proteins.

34 dentified TATA-Box Binding Protein (TBP) and Importin 8 (IPO8) to be stable in non-small cell lung ca

35 We have identified importin 8 as a factor that directly imports eIF4E into

36 We found that importin 8 is highly elevated in untreated patients with

37 Importin 8 only imports cap-free eIF4E.

38 ical step in its regulation and position the importin 8-eIF4E complex as a novel therapeutic target.

39 the action of dedicated transport receptors importin-9 and exportin-6, but how this transport is reg

40 r Formin-2 or actin's nuclear import factor, importin-9, increases the number of DNA double-strand br

41 RNAi screening of both importin alpha and beta family members, as well as co-im

42 Importin alpha and beta preferentially inhibit XCTK2 ant

43 only the open-form is capable of binding to importin alpha and that, upon binding, the 627 domain sa

44 These experiments identify importin alpha as a conserved surface area-to-volume sen

45 ition with DHBc for the host kinase, whereas importin alpha binding by CTD may contribute to inhibiti

46 Atomic structures of importin alpha bound to two variants of NLS2 revealed NL

47 Sequestration of importin alpha by GM130 liberates the spindle assembly f

48 tion of ChREBP.14-3-3 and inhibit the ChREBP/importin alpha interaction, resulting in cytosolic local

49 We show that the nuclear transport receptor importin alpha is modified by palmitoylation, which targ

50 importin alpha1 and discuss the evolution of importin alpha isoforms.

51 he nuclear import factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubi

52 Modulation of importin alpha palmitoylation in human cells similarly a

53 e altered by inhibitors that shift levels of importin alpha palmitoylation.

54 mportin alpha, and activated in stage 8 when importin alpha partitions to a membrane pool.

55 rained proteomimetics to target the HNF1beta-importin alpha protein-protein interaction were designed

56 Reconstitution of importin alpha targeting to the outer boundary of extrac

57 al nuclear localization signal that recruits importin alpha to the Golgi membranes.

58 he Golgi matrix protein GM130 interacts with importin alpha via a classical nuclear localization sign

59 ethylated H3 lysine 9 [H3K9me3]), and DIM-3 (importin alpha), which is involved in DIM-5 localization

60 n stage 3 spindles by the transport receptor importin alpha, and activated in stage 8 when importin a

61 try and repressor function, independently of importin alpha, its classical partner.

62 However, Nup50 dynamics are independent of importin alpha, Nup153, and Nup98, even though the latte

63 orresponding to NLS1 and NLS2 bind weakly to importin alpha, the two NLSs synergize in the context of

64 rimarily binds the major-NLS binding site of importin alpha, unlike NLS1 that associates with the min

65 red for efficient association between NP and importin alpha, which is crucial for IAV RNP nuclear tra

66 terminal half of the protein, which contains importin alpha- and Nup153-binding domains.

67 itotic NPC segregation is independent of its importin alpha- and Ran-binding domains but relies on a

68 facilitated by the nucleoporin Nup2 via its importin alpha- and Ran-binding domains.

69 We found that LHP1 interacts with importin alpha-1 (IMPalpha-1), importin alpha-2 (IMPalph

70 nteracts with importin alpha-1 (IMPalpha-1), importin alpha-2 (IMPalpha-2) and importin alpha-3 (IMPa

71 Palpha-1), importin alpha-2 (IMPalpha-2) and importin alpha-3 (IMPalpha-3) both in vitro and in vivo.

72 phages, NLS peptide specifically blocked the importin alpha-mediated nuclear import of NF-kappaB and

73 librium in the vicinity of the C-terminus of importin alpha.

74 We show that Xenopus Kinesin-14, XCTK2, and importin alpha/beta form an effector gradient that is hi

75 XCKT2 MT crosslinking activity by releasing importin alpha/beta from a bipartite nuclear localizatio

76 Although a classical NLS and importin alpha/beta mediated nuclear import pathway has

77 e actively trafficked to the nucleus via the importin alpha/beta pathway.

78 and inhibit nuclear import of importin beta, importin alpha/beta, and transportin cargoes in permeabi

79 ed with fluorescent cargos (mCherry) for the importin alpha/beta, transportin 1, and transportin 3 im

80 tosis by releasing the inhibitory effects of importin alpha/beta.

81 not with a mutant XCTK2 that cannot bind to importin alpha/beta.

82 ut rather generates effector gradients where importins alpha and beta gradually tune the activities o

83 l protein nuclear import pathway mediated by importin-alpha (Imp-alpha) and -beta1 (Imp-beta1).

84 owing that ivermectin targets the ability of importin-alpha (Imp-alpha) to recognize nuclear localiza

85 Coexpression of importin-alpha and inhibition of nuclear export by lepto

86 imatinib both blocked binding of PKCdelta to importin-alpha and nuclear import, demonstrating that ty

87 Cytoplasmic TDP-43 droplets slowly recruit importin-alpha and Nup62 and induce mislocalization of R

88 Cargo proteins interact with the importin-alpha armadillo repeat domain via nuclear local

89 mRPs and identified a repeat pair from yeast importin-alpha as having the optimal curvature geometry

90 Comparison of the importin-alpha binding affinities of HaRxL106 and other

91 d dimers adopted a conformation incapable of importin-alpha binding.

92 nuclear transport receptors belonging to the importin-alpha family in nucleolar accumulation of the P

93 KPNA7 is a member of the Importin-alpha family of nuclear import receptors.

94 se allele, impa-1(G146E), in one of the nine importin-alpha genes in the Arabidopsis genome.

95 binding assays, we show that it is bound by importin-alpha in almost the same manner and with simila

96 PMTV TGB1 interacted with importin-alpha in N. benthamiana, which was detected by

97 t and the amino-terminal domain required for importin-alpha interaction in plants, nucleolar targetin

98 siRNA knockdown of the human importin-alpha isoform KPNA2, corresponding to the murin

99 a isoform KPNA2, corresponding to the murine importin-alpha isoform previously shown to bind to DENV-

100 on studies using DENV-2 and -4 NS5 and human importin-alpha isoforms failed to identify an interactio

101 n (IFN) response by hijacking select nuclear importin-alpha isoforms.

102 os are preferentially imported by a distinct importin-alpha it remains unknown how this specificity i

103 However, owing to the lack of importin-alpha knockout animal models in the past, their

104 Importin-alpha mutants that impair interactions during n

105 the characteristic nuclear import defect of importin-alpha mutants, whereas srp1-49 shows a defect i

106 or importin-alpha, sequence variation at the importin-alpha NLS-binding sites and tissue-specific exp

107 ural armadillo repeat proteins (nArmRP) like importin-alpha or beta-catenin bind their target peptide

108 etermine redundant and specific functions of importin-alpha paralogs from Arabidopsis thaliana.

109 Virus-induced gene silencing of two importin-alpha paralogs in Nicotiana benthamiana resulte

110 Plant genomes typically encode several importin-alpha paralogs that can have both specific and

111 mediated in part by karyopherin-alpha (KPNA)/importin-alpha subtypes, regulates transcription factor

112 importin-alphas determine formation of cargo/importin-alpha transport complexes in plant cells.

113 Srp1 (importin-alpha) can translocate proteins that contain a

114 alization signals, recognized by the adaptor importin-alpha, and the PY nuclear localization signals,

115 Our results suggest that cargo affinity for importin-alpha, sequence variation at the importin-alpha

116 ortin-13) or through adaptor proteins (e.g., importin-alpha, snurportin-1, symportin-1), as well as u

117 sid (NC) domain of Gag and binds directly to importin-alpha, which recruits importin-beta to mediate

118 raction is needed for cell-to-cell movement, importin-alpha-mediated nucleolar targeting of TGB1 is a

119 Next, the vRNPs interact with importin-alpha/beta and enter the nucleus.

120 Finally, the known inhibitor of TPX2, the importin-alpha/beta heterodimer, regulates TPX2 condensa

121 nation of protein import, and disassembly of importin-alpha/CAS complexes after export occurs in the

122 the coordinated assembly and disassembly of importin-alpha/CAS complexes for generating productive t

123 TPase-activating factors to demonstrate that importin-alpha/CAS complexes form in the nuclear basket

124 The functions of importin-alpha/importin-beta and importin-11 have been v

125 lysine/arginine residues, unlike other known importin-alpha:beta-dependent NLSs.

126 311/321: EEPPAKRQCIE) that is recognized by importin-alpha:beta.

127 Binding of this domain to Importin alpha1 (Impalpha1) was confirmed by gel filtrat

128 A55 targets the host importin alpha1 (KPNA2), acting to reduce p65 binding an

129 romolar Kd, that is selective for the murine Importin alpha1 (mImpalpha1) minor site, with the Kd str

130 y of RCC1 for importin alpha3 vs the generic importin alpha1 and discuss the evolution of importin al

131 f magnitude higher affinity than the generic importin alpha1, although the two isoforms share an iden

132 CAS, exportin-2) and its transport substrate importin-alpha1 (imp-alpha1) among significantly up-regu

133 p32-dependent intracellular trafficking and importin-alpha1-mediated nuclear entry of AnkG.

134 c1 GTPase activity and is associated with an importin-alpha2 redistribution.

135 nd Rel-A, are translocated to the nucleus by importin alpha3 and importin alpha4.

136 We propose importin alpha3 evolved to recognize topologically compl

137 mains or are masked by quaternary structures.Importin alpha3 facilitates the nuclear transport of the

138 Here we identify the mechanisms of importin alpha3 selectivity for RCC1.

139 Importin alpha3 uses its greater conformational flexibil

140 ecular basis for the selectivity of RCC1 for importin alpha3 vs the generic importin alpha1 and discu

141 and beta-propeller, disrupts specificity for importin alpha3, demonstrating the structural context ra

142 t of Ran exchange factor RCC1 is mediated by importin alpha3.

143 The expression of importin-alpha3 and Rel-A in the lung of OVA-sensitized

144 reduced blood Tregs, increased expression of importin-alpha3 and Rel-A in the lung tissue.

145 A crystal structure of the importin-alpha3/MOS6 armadillo repeat domain suggests th

146 ocated to the nucleus by importin alpha3 and importin alpha4.

147 Further investigation indicated that, importin-alpha4 and importin-alpha7 directly interacted

148 ggest that upon DNA damage transport adaptor importin-alpha4 imports XPA into the nucleus in an ATR-d

149 he nuclear localization signal (NLS) of XPA, importin-alpha4 or/and importin-alpha7 are required for

150 Interestingly, the binding of importin-alpha4 to XPA was dependent on UV-irradiation,

151 R reduced the amount of XPA interacting with importin-alpha4.

152 in Kd obtains in binding studies with human Importin alpha5 (hImpalpha5), which in some cases has be

153 tion of TRIM59, leading to TRIM59 binding to importin alpha5 and nuclear translocation.

154 miR181b-3p modulates expression of importin alpha5 and sensitivity of TLR4-mediated signali

155 EF1D impaired the interaction between NP and importin alpha5 and the interaction between PB1 and RanB

156 This study identifies a miR181b-3p-importin alpha5 axis in regulating inflammatory signalin

157 the nuclear localization signal of ACSS2 for importin alpha5 binding and nuclear translocation.

158 Overexpression of miR181b-3p decreased importin alpha5 expression and normalized lipopolysaccha

159 181b-3p in liver and increased expression of importin alpha5 in nonparenchymal cells.

160 s, was identified as a target of miR181b-3p; importin alpha5 protein was increased in Kupffer cells f

161 Importin alpha5, a protein involved in p65 translocation

162 transactivator of the nuclear import factor importin-alpha5 (Impalpha5), and interfering with this m

163 on of karyopherin-alpha1 (KPNA1, also called importin-alpha5), which is known to mediate the nuclear

164 NLS in trans, which ensures high avidity for importin alpha7 while preventing non-specific binding to

165 he full length NP to confer high avidity for importin alpha7, explaining why the virus efficiently re

166 signal (NLS) of XPA, importin-alpha4 or/and importin-alpha7 are required for the XPA nuclear import.

167 tigation indicated that, importin-alpha4 and importin-alpha7 directly interacted with XPA in cells.

168 portin-alpha7 was not, suggesting a role for importin-alpha7 in nuclear translocation of XPA in the a

169 Here, we demonstrate that importin-alpha7 is involved in the formation of EBOV inc

170 However, deletion of the gene encoding importin-alpha7 was not sufficient to increase survival

171 dent on UV-irradiation, while the binding of importin-alpha7 was not, suggesting a role for importin-

172 Importin-alphas are essential adapter proteins that recr

173 tes and tissue-specific expression levels of importin-alphas determine formation of cargo/importin-al

174 in suggests that five of the six Arabidopsis importin-alphas expressed in rosette leaves have an almo

175 lear localization signal (NLS) that binds to importin and is required for its function during cytokin

176 f macromolecules through the nuclear pore by importins and exportins plays a critical role in the spa

177 ins of the karyopherin superfamily including importins and exportins represent an essential part of t

178 rosophila and discovered a critical role for importins and exportins, Ran-GTP cycle regulators, nucle

179 s of the karyopherin-beta superfamily termed importins and exportins.

180 om the ER membrane, making it accessible for importins and nuclear import.

181 place Klf6 at the nexus of a novel gp130-Klf-importin axis, which promotes differentiation and viabil

182 s RanGEF without perturbing cargo binding to importin beta and disrupts MI spindle function in chromo

183 Karyopherins, including importin beta and its cargo adaptors, have been shown to

184 We conclude that the cell contains importin beta and transportin "global positioning system

185 The nuclear import receptors importin beta and transportin play a different role in m

186 Here, we report that KPNB1, an importin beta component of the ncRNA repressor of nuclea

187 e a mechanism of EGFR regulation through the importin beta family member RAN-binding protein 6 (RanBP

188 ation analyses, revealed that a nonclassical importin beta family member, IPO3, was the only importin

189 t with IPO5, a nuclear import protein of the importin beta family, via a conserved nuclear localizati

190 GTP produced on chromatin frees factors from importin beta for localized assembly.

191 and there are the emerging roles for RanGTP/importin beta in ciliary protein targeting, we further i

192 cible inhibition of the conserved Drosophila importin beta in lateral neurons abolishes behavioral rh

193 Roles of Ran and importin beta in the coalescence of microtubule organizi

194 Importin beta is known to act by repressing assembly fac

195 biosensors to monitor the changes in Ran and importin beta signaling induced by perturbations of Ran

196 -regulating release of assembly factors from importin beta, and 2) direct action by transportin bindi

197 Here, we show that R-DPRs interact with importin beta, disrupt its cargo loading, and inhibit nu

198 cargo loading, and inhibit nuclear import of importin beta, importin alpha/beta, and transportin carg

199 nuclear transport receptors (NTRs), NTF2 and Importin beta, together with the concomitant film thickn

200 nd exportin 1 and involved the activation of importin beta-dependent nuclear import of 53BP1, a large

201 Soluble karyopherins of the importin-beta (impbeta) family use RanGTP to transport c

202 ta-arrestins (Arrbs), the small GTPase Rab5, importin-beta (Kpnb1), and transportin-1 (Tnpo1).

203 ur results suggest that rAAV2 interacts with importin-beta alone or in complex with other karyopherin

204 KPNA7 forms a complex with Importin-beta and facilitates the translocation of signa

205 The functions of importin-alpha/importin-beta and importin-11 have been verified in avia

206 signal (NLS) at its C-terminus that binds to importin-beta and is required for cortical polarity and

207 psid proteins from rAAV2 could interact with importin-beta and that this interaction was sensitive to

208 Of interest, the importin-beta binding (IBB) domain of SPN1, which is ess

209 5 nuclear localization, whereas knockdown of importin-beta did.

210 These data indicate that Ketel/importin-beta does not play a significant role in Drosop

211 extended our studies to other members of the importin-beta family and found that all tested karyopher

212 The importin-beta family members (karyopherins) mediate the

213 we show that transportin 1, a member of the importin-beta family proteins, binds to a PY-NLS(2) sequ

214 Msn5 and Los1, members of the importin-beta family, function in tRNA nuclear export.

215 of MKL1, which affects its interaction with Importin-beta for efficient nuclear import.

216 tazole, a small-molecule inhibitor of RanGTP/importin-beta function, to study the role of Ran in spin

217 3, two integral nuclear pore components, and importin-beta IMB-1 provides strong evidence that this r

218 mics simulations using crystal structures of Importin-beta in its free form or in complex with nuclea

219 other serotypes and found that the extent of importin-beta interaction varied, suggesting that differ

220 , small interfering RNA (siRNA) knockdown of importin-beta partially inhibited rAAV2 nuclear transloc

221 describe how microtubules and the RAN GTPase/importin-beta system collaborate to control timing of HU

222 s directly to importin-alpha, which recruits importin-beta to mediate nuclear entry.

223 icated that vertebrate snRNP import requires importin-beta, the transport receptor that binds directl

224 family, which can bind cargo directly (e.g., importin-beta, transportin-1, transportin-3, importin-13

225 d particles revealed that rAAV2 localized to importin-beta-dense regions of cells in late trafficking

226 GIR1 encodes an importin-beta-like protein required for the nuclear impo

227 r experiments using dual-fluorophore-labeled Importin-beta.

228 capsid severely inhibited interactions with importin-beta.

229 la SNUP (dSNUP) does not interact with Ketel/importin-beta.

230 ulators of Epac1 activity, we show here that importin beta1 (impbeta1) is an Epac1 binding partner th

231 an interaction of the C9-isoforms with both Importin beta1 and Ran-GTPase, components of the nuclear

232 ta-actin, GAP-43, Neuritin, Reg3a, Hamp, and Importin beta1 mRNAs.

233 Nuclear import of ErbB3 occurs via importin beta1, which drives the receptor through the nu

234 Huntingtin enters the nucleus via an importin beta1- and 2-dependent proline-tyrosine nuclear

235 ta-catenin cytoplasmic stabilization and the importin beta1/Ran-mediated transport system.

236 Analogous changes in importin-beta1, nucleoporin 98 and nucleoporin 62 nuclea

237 We show that although importin beta2 binding does not regulate anillin's funct

238 w that anillin is targeted to the nucleus by importin beta2 in a Ran-dependent manner through an atyp

239 Depletion of endogenous importin-beta5 associated with IQGAP1 also reduced expre

240 be a regulator of beta-catenin function via importin-beta5.

241 Thus, influenza A virus uses dual importin-betas for distinct steps in host cell entry.

242 Importin binding assays indicate that nuclear access occ

243 Here we reveal the mechanism whereby importin binding favors a conformation required for anil

244 tion of a cortical protein, we also show how importin binding positively regulates protein function.

245 Importin binding subsequently stabilizes a conformation

246 large-scale conformational changes prior to importin binding.

247 dle self-organization through the release of importin-bound spindle assembly factors (SAFs), which st

248 y of nuclear import pathways are mediated by importin-cargo interactions.

249 cellular dynamics, we developed in vivo beta-importin complex coimmunoprecipitation (co-IP) assays us

250 e mediated by direct binding of TRIM3 to the Importin complex.

251 mpair docking of cargo-receptor (karyopherin/importin) complex and disrupt nuclear import.

252 calisation signal)-cargo release from RanGTP-importin complexes.

253 In particular, we provide evidence for importin-dependent long-distance transport from synapto-

254 he perinuclear area and at the spindle in an Importin-dependent manner during cell cycle progression.

255 s revealed that Tsr2 efficiently dissociates importin:eS26 complexes via an atypical RanGTP-independe

256 lso used in fluorescence assays to find that importins facilitate the transport of signal-tagged albu

257 The evolutionarily conserved beta-importin family member Los1 (Exportin-t) has been the on

258 t in vivo biochemical evidence that two beta-importin family members, Los1 (exportin-t) and Msn5 (exp

259 dentified importin-5 (IPO5), a member of the importin family of nuclear transport proteins, as an int

260 belong to the evolutionarily conserved beta-importin family.

261 7:Impbeta:H1.0 complex, showing that the two importins form a cradle that accommodates the linker his

262 he histone chaperone Asf1b are important for Importin-histone recognition.

263 ition of VEEV capsid protein (C) by the host importin (IMP) alpha/beta1 nuclear transport proteins.

264 oteomics approach to identify members of the importin (IMP) family of nuclear transporters as interac

265 d nucleoporins Nup153 or Nup214 or some beta importins (Imp7 or Impbeta), it mediates the association

266 studies of H3 and H4 tails binding to seven Importins, Impbeta, Kapbeta2, Imp4, Imp5, Imp7, Imp9, an

267 ight into the structural flexibility of beta-importins in the unbound state.

268 s indicate that nuclear access occurs via an importin-independent mechanism.

269 Here, we identify a code that determines importin-independent nuclear import of ankyrin repeats (

270 DAR (RanGDP/AR) pathway represents a general importin-independent nuclear import pathway and is frequ

271 ecessitating the identification of a general importin-independent nuclear import pathway.

272 Treatment with ivermectin, an importin inhibitor, blocked HE4/importin-4 nuclear accum

273 pbeta dimerization and H1 import, resembling importin interaction with nucleoporins, which, in turn,

274 es NF-kappaB signaling by disrupting the p65-importin interaction, thus preventing NF-kappaB transloc

275 The nuclear importin IPO5 was identified as a novel interacting prot

276 f the mitotic inducer Cdc25 by targeting its importin, Kap123.

277 expression levels of an NF-kappaB-associated importin (KPNA4: one of the proteins responsible for the

278 which is transported to the cell body in an importin-mediated manner.

279 ition to revealing a novel mechanism for the importin-mediated regulation of a cortical protein, we a

280 7 nm, respectively, and binds the other five Importins more weakly.

281 In contrast, beta-importin Msn5 preferentially assembles with RanGTP and s

282 Yet not all nuclear proteins interact with importins, necessitating the identification of a general

283 ng elements overlap with the binding site of importins on TPX2.

284 nopore by nuclear transport receptors (e.g., importins) owing to their interactions with barrier-form

285 pproach to identify interactors of the yeast importin Pdr6/Kap122.

286 Therefore, the importin proteins IMPalpha-1, -2 and -3 are necessary fo

287 eins were chaperones, cytoskeletal proteins, importins, proteins involved in ubiquitination, kinases

288 Importin receptors are associated with nuclear transloca

289 lecular basis of the affinity of 627-NLS for importins remained unclear from these structures, appare

290 r localization sequence is a determinant for importin specificity.

291 ment of cells with 6 and 22 also upregulated importin subunit alpha-2 levels and repressed translatio

292 ortin beta family member, IPO3, was the only importin that was able to associate with NEMO and whose

293 ed transport receptors, called exportins and importins, that interact with cargo proteins in a RanGTP

294 lexibility therefore enables 627-NLS to bind importin through conformational selection from a tempera

295 3 and H4 are known to bind several different Importins to import the histones into the cell nucleus.

296 The importin Transportin-1 (TNPO1) plays a key role in the (

297 nding protein (CREB), and by an inhibitor of importin, which is required for activated CREB to get in

298 Our model is that during anaphase, "free" importins, whose gradient inversely correlates with acti

299 r two basic segments to bind the same set of Importins with a similar trend of relative affinities as

300 The specific interaction of importins with nuclear localization signals (NLSs) of ca