ライフサイエンスコーパス: in planta

1 ure enhanced BPM1 stability and accumulation in planta.

2 its impact, little is known about Al action in planta.

3 active sites to support carboxylation rates in planta.

4 protein complexes in monolignol biosynthesis in planta.

5 l pectic domain rhamnogalacturonan II (RGII) in planta.

6 reased protein stability of the PSR1 mutants in planta.

7 h wheat (Triticum aestivum) Actin1 (TaACT1), in planta.

8 regulated genes not detectably bound by NLP7 in planta.

9 ited by both Leptosphaeria spp. in vitro and in planta.

10 s is that they are found in very low amounts in planta.

11 at OsSQE2 is the preferred partner of OsONS1 in planta.

12 A pathway (ABI3, ABI4 and ABI5) was observed in planta.

13 n ATP-binding protein that formed homodimers in planta.

14 arily been investigated after overexpression in planta.

15 maturation into an active form in vitro and in planta.

16 broadly used to investigate effector biology in planta.

17 ith AMT1;1 and AMT1;2 in yeast, oocytes, and in planta.

18 Rx1 binds to NbGlk1 in vitro and in planta.

19 minor difference in sensitivity in vitro and in planta.

20 uenching of ROS by carnosic acid takes place in planta.

21 ng a novel processing mechanism is occurring in planta.

22 here, indicative of increased TCE metabolism in planta.

23 GIPC glucuronosylation could not be analyzed in planta.

24 tween ER bodies and glucosinolate metabolism in planta.

25 the formation of mature and bioactive CLE40 in planta.

26 effectors enabled direct effector detection in planta.

27 graminearum is reorganized both in vitro and in planta.

28 n remain incompletely understood, especially in planta.

29 that SOT1 is responsible for their formation in planta.

30 nd easy platform for assessing gene function in planta.

31 on "gatekeeper" Tyr both in vitro as well as in planta.

32 thod to investigate the RNA binding proteome in planta.

33 y is integrated into the complex HSR network in planta.

34 uptake and sunlight-triggered release of T6P in planta.

35 ECROW transcriptional cascade were validated in planta.

36 genic fruiting bodies and GFP was detectable in planta.

37 t have a higher probability of being present in planta.

38 ntion strategy directly modulates T6P levels in planta.

39 t the Bradi5g03300 UGT converts DON into D3G in planta.

40 ies of self-regulated auxin-based patterning in planta.

41 und state and explaining its weaker activity in planta.

42 tissue-specific expression of BAS1 and SOB7 in planta.

43 ng an absolute requirement for all cysteines in planta.

44 inciding with strongly reduced fungal growth in planta.

45 detect and analyze PCD processes in vivo and in planta.

46 pporting a role for PSKR1 signaling via cGMP in planta.

47 ng in reorganization of the COP1/SPA complex in planta.

48 s and over-expression lines of CGR2 and CGR3 in planta.

49 as FUL) and ARF proteins directly associate in planta.

50 b) directly interacts with RACB in yeast and in planta.

51 he ectopic expression of specific antibodies in planta.

52 nces colonization when transiently expressed in planta.

53 pk23 confirmed the regulatory role of CIPK23 in planta.

54 confirm the N and C terminus of the peptide in planta.

55 dulating lignin composition and/or structure in planta.

56 t role of a phenylpropanoid coupling product in planta.

57 PP2A constitutively associates with BAK1 in planta.

58 e TLS1 protein facilitates the movement of B in planta.

59 Specific interactions were verified in planta.

60 he long-distance translocation of cytokinins in planta.

61 n the nematode and insect molecules secreted in planta.

62 8, which interacts with AvrPtoB in yeast and in planta.

63 confers transcriptional activation activity in planta.

64 d development by affecting auxin homeostasis in planta.

65 or stably fine-tuning residual gene function in planta.

66 notyping data from synthetic peptide screens in planta.

67 hed the accumulation of sulfated salicinoids in planta.

68 ultifunctionality and the structure of cutin in planta.

69 gesting that they interact to form a complex in planta.

70 al precursor, strictosidine, to various TIAs in planta.

71 te with other bacterial species in vitro and in planta.

72 he unannotated ORFs generate stable proteins in planta.

73 the analysis of protein-protein interactions in planta.

74 interacts with herbivory-induced CALMODULIN2 in planta.

75 a heterologous experimental system and also in planta.

76 in Aphis gossypii, Ag10k, interact with TFT7 in planta.

77 O IV, and traces of CO V, both ex planta and in planta.

78 tudy the functional status of xylem networks in planta.

79 zes with components of the decapping complex in planta.

80 ication of chemical specificity on autophagy in planta.

81 er midgut surface of insect vectors, but not in planta.

82 ontaining proteins able to induce cell death in-planta.

83 To check this mechanism in planta, a benzyl etherification of nonesterified hydr

84 by the legal scheduling of Cannabis, the low in planta abundances of nearly all of the dozens of know

85 ay a role in the regulation of HPR1 activity in planta, although it was not required for growth under

86 In planta analyses revealed that AVR1 and Sec5 are in cl

87 However, detailed in planta analyses suggest that the biosynthesis of 2,3-

88 nce its inception, the available options for in planta analysis are still subject to very low signal-

89 In planta analysis of IAA, PAA, SA, and BA and their res

90 Using in planta ancestral protein reconstruction, we demonstra

91 , we investigated the role of the ACT domain in planta and confirmed its involvement in chloroplast d

92 FDH is ubiquitinated in planta and degraded by the 26S proteasome.

93 m avrBs2 and xopX both showed reduced growth in planta and delayed spread through the vasculature sys

94 es, no relationship was seen between fitness in planta and either the magnitude of their expression i

95 ng disease and its pathogen was investigated in planta and fungal growth and sporulation production w

96 ary evidence obtained through gene silencing in planta and heterologous expression in bacteria suppor

97 diates, complementing and extending previous in planta and in vitro investigations.

98 Functional studies (in planta and in vitro) show that Vv3AT has a broad anth

99 We evaluated, both in planta and in vitro, how whitefly infestation affects

100 their roles have been difficult to decipher in planta and in vitro.

101 on pathway and interact physically in vitro, in planta and in wheat cells.

102 or immunity and protein-protein interactions in planta and in yeast (Saccharomyces cerevisiae).

103 on triggers Sr50-dependent defense responses in planta and interacts directly with the Sr50 protein.

104 of its antioxidative features, this compound in planta and its antioxidant mechanism have received li

105 Excess Mn also increased the interaction in planta and led to greater accumulation of the complex

106 on how the levels of tyrosine are controlled in planta and linked to overall growth and development.

107 nse-related Accelerated Cell Death11 (ACD11) in planta and promotes the proteasome-dependent turnover

108 ell defined toolkit for H(2) O(2) monitoring in planta and showed that intracellular H(2) O(2) measur

109 entroid vectors is significantly affected by in planta and soil concentration gradients and when conc

110 s to a dissolution of the low soluble CeO(2) in planta and subsequent precipitation.

111 We show that HsGCPII cannot substitute AMP1 in planta and that an HsGCPII-specific inhibitor does no

112 provides fitness and colonization advantages in planta and that the role of the T6SS is not restricte

113 hese genes can determine anthocyanin content in planta and, hence, fitness under UV-B irradiation str

114 -19-ol and germacrene-d-4-ol were detectable in planta, and gene expression analysis of the biosynthe

115 ecretome of X. fastidiosa, both in vitro and in planta, and identified LesA as one of the pathogenici

116 ependent interbacterial competition activity in planta, and the C-terminal variable region of VgrG2 g

117 led structure, the function of these glycans in planta, and the mechanisms by which they are depolyme

118 d MLA10 can self-associate both in vitro and in planta, and this self-association correlates with the

119 erformed to validate one of the interactions in planta, and virus-induced gene silencing was used for

120 functional roles of individual NCR peptides in planta are not known.

121 In planta as in the moss Physcomitrella patens protoplas

122 cal relationship appears to be very specific in planta, as other fatty acids (FA) desaturases do not

123 -spread conidia infect ash and may sporulate in planta, as well as in forest debris.

124 ive site and tested these by mutagenesis and in planta assays of necrosis induction by expression in

125 In vitro and in planta assays showed that unlike SrCPS and SrKS1, SrC

126 ity of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and d

127 lization and quantification of fungal growth in planta at early infection stages surpassing visualiza

128 mall group of endogenous molecules affecting in planta auxin concentrations.

129 In planta, auxin is the first hormone group that was dis

130 PYL6 and MYC2 interact in planta based on bimolecular fluorescence complementat

131 PGIPs are predominantly studied in planta because their heterologous expression in micro

132 overed, many of which have not been reported in planta before.

133 In planta bimolecular fluorescence complementation assay

134 SIS8 based on a yeast two-hybrid screen and in planta bimolecular fluorescence complementation.

135 t broader DNA structural features may define in planta binding.

136 d soil microbiome analysis with in vitro and in planta bioassays to show that competition for iron vi

137 ing an integrated approach to understand the in planta biotransformation of KAuCl4 into AuNPs.

138 -1 knockout mutant background revealed that, in planta, both forms are negative regulators of abscisi

139 ited plant callose biosynthesis in vitro and in planta but also partially restored virulence to the D

140 is necessary for natural rubber biosynthesis in planta, but yeast-expressed CPTL2 and CPT3 alone coul

141 Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI)

142 content-specifically, RNAs 3 and 4-assembled in planta by agrobacterium-mediated transient expression

143 ngly suggesting that the antibiome expressed in planta by B. amyloliquefaciens does not reflect the v

144 ree Gbetagamma dimers at the plasma membrane in planta by bimolecular fluorescence complementation.

145 These interactions were confirmed in planta by FLIM-FRET and BiFC and the roles of SR34 do

146 o test the hypothesis that OGs are generated in planta by partial inhibition of pathogen-encoded poly

147 we verified that a similar trimer is formed in planta by the common bean (Phaseolus vulgaris) NF-Y s

148 n yeast or Xenopus laevis oocytes, and their in planta cellular and subcellular localization.

149 By in planta co-expression of tyrosyl protein sulfotransfer

150 However, in an in planta coinfection assay, A. tumefaciens used Tde eff

151 that are transcriptionally regulated by LEC1 in planta Collectively, our results show that LEC1 contr

152 expression in planta or degree of induction in planta compared to in vitro conditions measured in ot

153 Results indicate that in planta concentrations are significantly and positivel

154 In planta concentrations of these compounds strongly inh

155 The biofilms formed by the exDNase mutant in planta contained more and thicker fibres.

156 Therefore, in planta, CUS1 can catalyze the esterification of both

157 certain types of cells at defined stages of in planta development for in-depth analysis.

158 The altered Ce in planta distribution was partially associated with the

159 We have recently provided the first direct in planta evidence for polyspermy induced polyploidizati

160 ely induced programmed cell death, providing in planta evidence of allosteric CNGC regulation by CaM.

161 d us to assemble each virion type separately in planta Experimental approaches analyzing the morpholo

162 Some in planta experiments designed to test the validity of P

163 In planta expression identified an effector, with an N t

164 In planta expression of a thermophilic endoglucanase (Ac

165 Elevated in planta expression of resistance-type Rhg1 alpha-SNAPs

166 In planta expression of SAUR-immune pp2c.d2 or pp2c.d5 d

167 Here, we show that in planta expression of the RXLR effector Pi04314 enhanc

168 Enzyme kinetics, in planta expression, lignin structural analysis, and im

169 transient Agrobacterium tumefaciens-mediated in planta expression, transformation of P. infestans wit

170 In planta feeding of C(14) or C(13)-labelled tZ suggests

171 mutualist services to hosts and had superior in planta fitness during clonal infections, consistent w

172 Here, we assessed in planta flg22-signaling competency in the context of l

173 We used in planta fluorescence lifetime imaging microscopy and f

174 s including yeast two-hybrid, pull-down, and in planta fluorescence resonance energy transfer assays,

175 have broad application as it determines the in planta flux control that a single component has upon

176 ort, we scrutinized the requirement of Rad54 in planta for two distinct fully infectious geminiviruse

177 analysis of E. lathyris L. mature seeds and in planta functional characterization, we identified thr

178 y characterized at biochemical levels, their in planta functions remain uncertain.

179 on of ligands and discriminate between their in planta functions.

180 his enzyme variant can produce bioactive GAs in planta Furthermore, a genetically modified GA3ox5 var

181 ation of acetolactate synthase1 gene through in planta gene editing.

182 The in planta generation of one of the most complex human pr

183 and AtBRCA1 physically interact in vitro and in planta Genetic interaction between the RBR-silenced a

184 that had not been previously associated with in planta growth were also required for maximum epiphyti

185 wall of plant-pathogenic fungi during on and in planta growth, following the elucidation of infection

186 large aggregates without the cognate Se SSU in planta, harboring active Rubisco that enables plant g

187 xidants; however, their antioxidant activity in planta has been debated.

188 hanism of heme-dependent inhibition of GluTR in planta has remained elusive.

189 In this study we determined that the in planta heterologous expressed OeGLU, an oleuropein-sp

190 Such high specialization of class II CPRs in planta highlights the evolutionary strategy that ensu

191 Cutin influences many biological processes in planta; however, due to its complexity and highly bra

192 protein interaction resource, obtained using in planta immunoprecipitation (IP) followed by mass spec

193 to the nucleus and interact with each other in planta in bimolecular fluorescence complementation an

194 length Sr33 and Sr50 proteins self-associate in planta In contrast, truncated CC domains equivalent i

195 ical and biophysical techniques in vitro and in planta, including kinase assays, microscale thermopho

196 ndirect effects of BAR-containing transgenes in planta, including modified amino acid levels, have be

197 In planta, increasing NEDD8 gene dosage is sufficient to

198 Subcellular localization in planta indicated that AtPyrP2 was localized in plasti

199 In planta-induced mobilization of HAI2 was regulated by

200 In planta interactions between UVR8 and PIF5 are not obs

201 ic profiling revealed that MDCA is converted in planta into piperonylic acid (PA), an inhibitor of CI

202 In planta, IPK acts in parallel with the MVA pathway and

203 ant metabolism for colonization and survival in planta Kiwellin (KWL) proteins are a widespread famil

204 des a dysfunctional protein that accumulates in planta like wild-type PEN3.

205 mass spectrometry analysis, we show that the in planta mature form of proGrCLE1, a multidomain CLE ef

206 operties of the sensor that are critical for in planta measurements, including specificity, pH stabil

207 Here we present an efficient in planta method for Agrobacterium-mediated genetic tran

208 on of genetic factors associated solely with in planta mobilization of an ICE demonstrates that this

209 ato mutants, were performed to elucidate the in planta molecular mechanisms involved in induced resis

210 RY2 with COP1 in yeast two-hybrid assays and in planta Mutations in the VP motif of CRY2 abolished th

211 In summary, we established a technique for in planta NAD redox monitoring to deliver important insi

212 In planta, NatB complex formation was essential for enzy

213 Both antibodies were efficiently sulfated in planta on coexpression of an engineered human tyrosyl

214 tations on protein function and localization in planta, on metal-binding properties in vitro and on p

215 factors that participate in gene expression in planta or are suspected to be involved in that proces

216 and either the magnitude of their expression in planta or degree of induction in planta compared to i

217 ymes and the improvement of MIA availability in planta or in vitro.

218 be useful in the engineering of anthocyanins in planta or in vitro.

219 Unlike previous in planta or intra-genomic homologous recombination repo

220 end the duration of photoreceptor activation in planta Our findings show that slowing the phototropin

221 We have developed an in planta packaging assay based on the transient express

222 s recent progress in understanding SL action in planta, particularly in the context of the regulation

223 zation of recombinantly produced enzymes and in planta peptide cyclization assays, we define the mole

224 ishes enhanced leaf colonization mediated by in planta Pi04314 expression.

225 e interaction between cryptochromes and Cop1 in planta, pointing to a common ancestor in which the cr

226 tive kinases in vitro but are phosphorylated in planta, possibly by an unknown kinase.

227 nt or pathogen cues triggers pathogen growth in-planta post penetration.

228 Genetic analysis shows that both in planta PR gene expression and release of elicitors ar

229 otein profiling analyses documented that the in planta presence of 4E02 does not impede enzymatic act

230 ue contribution SiMPull is poised to make to in planta protein interaction studies.

231 wledge validated here for the first time for in planta quantitation of biopharmaceuticals, is especia

232 The functional roles of XIP in planta remain poorly identified.

233 for the regulation of nuclear-encoded genes in planta remains to be explored.

234 n insight into their capacity for moving Suc in planta, representative members of each clade were fir

235 )-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at

236 gulate 76% and 87% of TF(1) indirect targets in planta, respectively.

237 Our in planta results show that OPT3 is important for leaf p

238 This is the first study to employ in planta RNAi approach to target the Rs-cps gene for th

239 To evaluate the effect of in planta RNAi on the control of this nematode, a specif

240 ss III peroxidases (PRXs) in plants, but the in planta role of most members of this family still rema

241 Next we showed that in planta sgRNAs bound to Cas9 are devoid of the expecte

242 RNA sequencing analysis in planta showed that SnTox1 was differentially expresse

243 Here, we perform in planta silencing efficacy assays on seven Arabidopsis

244 ction than wild-type plants, and vice versa, in planta silencing of MjTTL5 substantially increased pl

245 subfamily of GPCRs, with many of them having in planta-specific upregulation and a common promoter el

246 Knowledge of thapsigargin in planta storage and biosynthesis has been limited.

247 In vitro and in planta studies demonstrate that P2K2 and P2K1 interac

248 In planta, such monolignol-pCA conjugates become incorpo

249 ased when co-expressed with a functional KEG in planta, suggesting that KEG contributes to FDH degrad

250 Nevertheless, bioassays using an in Planta System showed that the Asian citrus psyllid wa

251 d quantitative proteomics, we established an in planta system that enables rapid study of MPK4 signal

252 er bioenergy crops to accumulate bioproducts in planta that can be fractionated and recovered at bior

253 e three proteins may be present in a complex in planta that is required to coordinate a correct photo

254 We found that, in planta, the constitutively active, GTP-bound AtGPA1(Q

255 expression analyses in mutants reveal that, in planta, the majority of these regulators repress the

256 is intact when AvrRpt2 is directly expressed in planta These observations led us to discovery of a ne

257 ns made in yeast, Lr67res reduced Glc uptake in planta These results confirm that the pathway by whic

258 als retention of ATG8CL binding in vitro and in planta This study offers new insights into structure/

259 ow our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the st

260 ochemical assays and heterologous expression in planta to demonstrate that TcADH2 encodes an enzyme t

261 of PCBER is to reduce phenylpropanoid dimers in planta to form antioxidants that protect the plant ag

262 dies revealed that SPT known to be activated in planta to SPT-enol acts as a developmental inhibitor

263 ates the auxin-responsive PIN3 transcription in planta to steer the early steps of lateral root forma

264 bunit Regulatory Particle AAA-ATPase6 (RPT6) in planta to suppress proteasome activity, resulting in

265 We developed a novel in planta transcriptional activation/repression assay an

266 tudy, particularly rhizosphere interactions, in planta transformations, and physicochemical propertie

267 ining mechanisms of uptake and accumulation, in planta transformations, the effects of PPCPs on plant

268 g a Tobacco rattle virus-derived plasmid for in planta transient expression of double stranded RNA (d

269 In planta transient expression of NnCYP76B6 showed a sig

270 Furthermore, the in planta transient expression of these three selected n

271 In planta transient over-expression of WsMYC2 showed sig

272 In planta transport assays demonstrate that PIC30 specif

273 with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX.

274 f possible co-evolution with host plants and in-planta up-regulation in particular, aided identificat

275 at these sites is substantially more stable in planta upon photoconversion to Pfr and is hyperactive

276 Here we describe the in planta use of carbon-based nanoparticles produced by

277 itute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts.

278 asive hyphae was required for optimal growth in planta Using a multicell model of fungal hyphae, we s

279 ysis of three PLRV-interacting host proteins in planta using a reverse-genetics approach revealed a c

280 um); (2) confirmation of the identified hits in planta using Arabidopsis (Arabidopsis thaliana); (3)

281 However, the genetic determinants underlying in planta variation of cannabinoid alkyl side-chain leng

282 pHapo was monitored in planta via microscopy-based ratio imaging, and the le

283 in cells to indirect targets responding only in planta via Network Walking.

284 on, length, motility, biofilm formation, and in planta virulence.

285 m Arabidopsis and rice transiently expressed in planta, we demonstrate that a urease-UreD-UreF-UreG c

286 In yeast (Saccharomyces cerevisiae) and in planta, we further demonstrated by both coimmunopreci

287 Here, through a forward genetic screen in planta, we identify a conserved amino acid residue th

288 omposition of developing embryos match those in planta were used to quantify substrate uptake and res

289 at BAK1 and SOBIR1 associate with each other in planta when the function of BIR1 is compromised.

290 ogical relevance of the CaM-based regulation in planta, where stomatal closure, induced by exogenous

291 ine and 2-phenylethyl-beta-d-glucopyranoside in planta, whereas PtAAS1 likely contributes to the herb

292 function, but not for localization, of HMA4 in planta, whereas the Glu residue is important but not

293 f infection structures and was nonpathogenic in planta, which was partially recovered by addition of

294 PRR5 can be O-fucosylated by SPY in planta, while point mutation in the catalytic domain

295 Likewise, ACHT4 reacted in planta with 2-Cys Prx, indicating that it is oxidized

296 Pi04089 interacts in yeast and in planta with a putative potato K-homology (KH) RNA-bin

297 lant cell periphery, interacted in yeast and in planta with multiple receptor-like kinases, including

298 Furthermore, DKM can interact in yeast and in planta with proteins involved in shoot apical meriste

299 the host nucleus and interacts in yeast and in planta with StUBK.

300 orylation occurs at multiple serine residues in planta, with S258 phosphorylation significantly reduc