ライフサイエンスコーパス: in-frame

1 P proteins-an expected outcome with overlaps in frame -2.

2 sequences but were separated from them by an in-frame "2A-like" sequence element that specifies a cot

3 rame of a gene in the genome and analyze the in-frame (3n) and frameshift (3n + 1 and 3n + 2) mutatio

4 is non-essential, the cells would carry both in-frame (3n) and frameshift (3n + 1 and 3n + 2) mutatio

5 In contrast, the cells would carry only in-frame (3n) mutations if the targeted gene is essentia

6 ters metabolism by cleaving transcripts with in-frame 5'-AAA-G/A-3' sites.

7 ersions in exons 5 and 8, respectively), one in-frame 5-11 microindel in exon 7 and a 410-bp deletion

8 rsions in PRKCA mRNAs (out-of-frame 61 bp or in-frame 66 bp long), and is preferentially included in

9 ine 95 to histidine (P95H) and a P95-to-R102 in-frame 8-amino-acid deletion caused significant change

10 in reorganization within the gorge, freezing-in-frame a conformation distinct from an unbound state o

11 When a non-phase-variable, in-frame allele of FA1090 opaD was reintroduced into Opa

12 RNAs identified key protein interfaces where in-frame alleles resulted in loss-of-function due to des

13 diate phenotypes that co-segregated with the in-frame alleles.

14 different putative knockout alleles and two in-frame alleles.

15 erdisciplinary collaboration that we pursued in framing and completing both projects.

16 leaves a short DNA footprint that can create in-frame and frameshift insertions in coding sequences.

17 We introduced and characterized in-frame and out-of-frame insertions and deletions in th

18 ns, whose synthesis is initiated at internal in-frame and out-of-frame start sites, can be functional

19 57BL/6-Klk4 (+/LacZ) mice have LacZ inserted in frame at the Klk4 translation initiation site so that

20 lation initiation codon and rely on a second in-frame ATG codon to produce an enzymatically active is

21 as suggested by the significant bias against in-frame ATGs specifically found at the beginning of the

22 tochondrial Arf") initiated from an internal in-frame AUG codon specifying methionine-45.

23 hat are translated from the second and third in-frame AUG codons in the NS1 open reading frame.

24 We also show that insertion of an in-frame AUG start codon upstream of the interaction sit

25 roduced from the initiation at a downstream, in-frame AUG start codon.

26 codon is followed by a strong Kozak context in-frame AUG, separated by mitochondrial-targeting seque

27 ized in the nucleus, and mutation of the two in-frame AUGs has no effect on the activation of IRF3 an

28 NS1 protein containing mutations of the two in-frame AUGs results in both the absence of truncated N

29 xA gene was modified to encode toxin with an in-frame beta-lactamase (Bla) fusion.

30 WES and BRAF sequencing identified in-frame BRAF deletions in the beta3-alphaC loop in 6 le

31 arrangements in human ALCL are predominantly in-frame, but often aberrant, with clonal TCRalpha but n

32 genomic ATP7 rescue construct containing an in-frame C-terminal GFP tag.

33 Most Ewing sarcomas are characterized by the in-frame chromosomal translocation t(11;22) generating t

34 tRNA(Lys19-CUU), ribosome stalling occurs at in-frame cognate AAA Lys codons.

35 In-frame complex indels are enriched in PIK3R1 and EGFR,

36 out of 9 TBDR coding genes were individually in-frame deleted.

37 med PtaA for "periplasmic transaminase A" An in-frame-deleted ptaA mutant selectively lacked the alph

38 The analysis of in frame deletion mutants confirmed the role of a hydrox

39 We identified one in-frame deletion (Ala221del) and one rare missense vari

40 es, the mutation identified was a novel 3-bp in-frame deletion (c.20_22del) that results in deletion

41 ation in CAPN3 could be identified; a 21-bp, in-frame deletion (c.643_663del21).

42 ovo, one inheritance uncertain), and Lys2596 in-frame deletion (four de novo).

43 o the presence or absence of a 12-nucleotide in-frame deletion (Fpr3Delta424-435).

44 h a missense (p.Ala368Thr or p.Leu370Pro) or in-frame deletion (p.Val618_Lys619del) in RINT1.

45 ted sequencing, here we identify a recurrent in-frame deletion (VAV1 Delta778-786) generated by a foc

46 oth the rare truncating mutations and common in-frame deletion alteration of FAM190A may contribute t

47 DNA microarray analysis of biofilms of pdeB (in-frame deletion and EAL) mutant cells revealed that ex

48 ound heterozygous mutations consisting of an in-frame deletion I81_K84 and an A500V mutation, and the

49 = 8.3 x 10(-55), beta = -0.90 s.d.), a rare in-frame deletion in FCGR2B abolishing IgG binding to th

50 al and behavioral bases of an ASD-associated in-frame deletion in hDAT at N336 (DeltaN336).

51 quencing revealed a recessive germline 21-bp in-frame deletion in NUAK2 segregating with the disease.

52 analysis demonstrated an association with an in-frame deletion in NUDT15 (chromosome 13, rs746071566)

53 of BAP1 that disrupt the CUBI and notably an in-frame deletion in the CTD that inhibits its interacti

54 EGFRvIII results from an in-frame deletion in the extracellular domain of EGFR, d

55 that is highly correlated with a well-known in-frame deletion in the gene.

56 We demonstrate via in-frame deletion mutagenesis of two different S. equi s

57 d pneumonic plague, we characterized an msbB in-frame deletion mutant incapable of producing an acylt

58 Here, we generated an unmarked, in-frame deletion mutant of csrA to assess its contribut

59 Interestingly, an in-frame deletion mutant of Irr shows no major role in i

60 In-frame deletion mutation (Del-L955) in NaV1.7 sodium c

61 cent studies show that a naturally occurring in-frame deletion mutation (Del-L955) of NaV1.7 channel,

62 Recently, an in-frame deletion mutation (DeltaE81) in a conserved reg

63 e and FBM, our data provide an example of an in-frame deletion mutation exerting a 'polar' effect on

64 ronchiseptica strain RBX9, which contains an in-frame deletion mutation in fhaB, encoding filamentous

65 We constructed an in-frame deletion mutation in rpoN (VP2670) in V. paraha

66 The in-frame deletion mutation was found to be inherited fro

67 Less common point and in-frame deletion mutations at codons 591 to 603 remain

68 In-frame deletion mutations of all of the ctp genes, wit

69 e role of these genes in pathogenesis, large in-frame deletion mutations of bcaA and bcaB were constr

70 Mutants with in-frame deletion mutations of two of the genes identifi

71 entified four mutations (three missense, one in-frame deletion of 30 base pairs) in six primary tumou

72 assays, and resulted in exon skipping and an in-frame deletion of 40 amino acids in primary human fib

73 Col6a3 mRNA and a mutant transcript with an in-frame deletion of 54 bp of triple-helical coding sequ

74 ely spliced F5 transcript that results in an in-frame deletion of 702 amino acids of the large activa

75 The genomic deletion corresponds to an in-frame deletion of 79 amino acids, shortening the prot

76 The deletion corresponds to an in-frame deletion of 92 amino acids, shortening the prot

77 al hypocalciuric hypercalcemia type 2 had an in-frame deletion of a conserved Galpha11 isoleucine (Il

78 DYT1 dystonia is caused by an in-frame deletion of a glutamic acid codon in the gene e

79 ipping of exon 6 of SLC41A1, resulting in an in-frame deletion of a transmembrane helix.

80 C. muridarum transformants with an in-frame deletion of either pgp3 or -4 but not -7 failed

81 One patient had an in-frame deletion of exon 13, while the second patient h

82 One mutation, c.1843-1G>A, results in an in-frame deletion of exon 13.

83 hat a splice isoform of erbB2, containing an in-frame deletion of exon 16 (herein referred to as ErbB

84 This mutation results in an in-frame deletion of exon 8, which results in non-functi

85 An in-frame deletion of feoA, feoB, or feoC eliminated iron

86 ble hypomorphic allele (xiamen [xm]) causing in-frame deletion of four amino acids from the interswit

87 A mutant containing an in-frame deletion of the bpsABCD structural genes was co

88 Furthermore, we showed that in-frame deletion of the cdaA gene in S. mutans causes d

89 and the T9SS, mutants were constructed with in-frame deletion of the CTD and deletion of porU, a C-t

90 In-frame deletion of the ctpA pilin gene in the ctpCD, c

91 Here we present the first in-frame deletion of the PvdN-encoding gene.

92 The in-frame deletion of the S. mutans frtR gene resulted in

93 CRISPR/Cas9 to create nematodes carrying an in-frame deletion of the same 571-residue portion of the

94 In-frame deletion of these genes impaired in vitro growt

95 An in-frame deletion of this regulator caused P. aeruginosa

96 Nematodes with this in-frame deletion show defective locomotion and muscle f

97 Creation of an S. oneidensis in-frame deletion strain showed increased sensitivity to

98 A pathogenic RYR2 exon 3 in-frame deletion was identified in the third proband di

99 within the functional domains, and one small in-frame deletion was identified.

100 plice donor site in exon 11, and leads to an in-frame deletion within the prelamin A mRNA and the pro

101 e splice site, six missense variants and one in-frame deletion) and one family with a missense varian

102 N (substitution), lasR (deletion), and rpoD (in-frame deletion), all encoding regulators of large gen

103 Each of these homozygous variants (a large in-frame deletion, a frameshift deletion, and a missense

104 ng an exon-skipping mutation that induced an in-frame deletion.

105 he encoded protein, inducing a frameshift or in-frame deletion/insertion of multiple residues.

106 tation in IKBKG (c.518+2T>G, resulting in an in-frame deletion: p.DelQ134_R256).

107 Strains harbouring in frame deletions of all V. cholerae genes that are pre

108 e was replaced by genomes harboring internal in-frame deletions affecting the L- or capsid-coding reg

109 600E harbored somatic mutations in MAP2K1 (6 in-frame deletions and 1 missense mutation) that induced

110 De novo in-frame deletions and duplications in the SPTAN1 gene,

111 In-frame deletions and enzyme activity assays were used

112 ) of DIPGs, including missense mutations and in-frame deletions and insertions not previously describ

113 Markerless in-frame deletions confirmed that clrA, clrB and clrC we

114 however, biallelic mutations involving small in-frame deletions did not induce the anephrogenic pheno

115 In-frame deletions frequently caused dominant retinal de

116 All 12 patients with in-frame deletions had a stable transcript compared with

117 o test strains with transposon insertions or in-frame deletions in biofilm-associated loci: ahrC, arg

118 In-frame deletions in BMD and a DMD non-silent mutation

119 Homozygous frameshift or in-frame deletions in CD70 in these patients abolished e

120 In-frame deletions in ORF69 have varied effects on NEC f

121 ilis epsH-K genes into Escherichia coli with in-frame deletions in the PNAG biosynthetic genes pgaA-D

122 or isolated dystonia by inducing a series of in-frame deletions in zebrafish embryos.

123 e activity has been described, the effect of in-frame deletions is not well understood.

124 reduced by 50% have been proven for specific in-frame deletions of 1 or several amino acids, probably

125 A transcripts in 40% of cancers also contain in-frame deletions of evolutionarily conserved exons.

126 Moreover, in-frame deletions of one hydroxylase and two P450 monoo

127 In-frame deletions of three or more codons conferred at

128 r, many tumors exhibit missense mutations or in-frame deletions or insertions, often outside the func

129 Corresponding in-frame deletions presented with predominantly mild BMD

130 Mutations were missense or small in-frame deletions that affect amino acid residues withi

131 Small in-frame deletions within ORF67 in all cases result in l

132 trophinopathy patients containing equivalent in-frame deletions.

133 uded seven unique missense variants and nine in-frame deletions/duplications of which 12 were novel.

134 ing transcripts, and approximately 20-30% of in-frame Delta9,10 transcripts predicted to encode a BRC

135 Although most children showed a decrease in frame dependency over the 4 years of the study, almos

136 or mutant tarP alleles engineered to harbor in-frame domain deletions.

137 We identified two novel in-frame Drosha isoforms generated by alternative splici

138 the microscope over the imaged area, warping in frames due to changes in contact angle and varying re

139 ion hotspots map to nonstructural genes with in-frame duplications at gene boundaries.

140 riants, including nonsense variants, induced in-frame E12 skipping through the modification of splice

141 probing of the putative linker segment using in-frame enhanced green fluorescent protein (EGFP) inser

142 man CVID-affected family with a heterozygous in-frame exon 9 skipping mutation (c.835+2T>G) and in a

143 tory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin.

144 (Rb) tumor suppressor protein (RbN) harbors in-frame exon deletions in partially penetrant hereditar

145 nous domain of collagen VII is encoded by 82 in-frame exons, which makes splice-modulation therapies

146 RNA-seq revealed one case with an in-frame FAM73A-BRAF fusion lacking the BRAF autoinhibit

147 Our analyses identified 331, 303 and 667 in-frame FGs with retaining kinase, DNA-binding, and epi

148 We used a "cDNA in-frame fragment library" screening approach to identif

149 This in-frame frtR mutant was used for RNA-sequencing and the

150 /Mental Retardation, X-linked) that generate in-frame fusion (IFF) proteins devoid of key chromatin i

151 Moreover, we predicted six in-frame fusion genes at sequenced duplication breakpoin

152 ed IL-1Ra (PAS-IL-1Ra) has been generated by in-frame fusion of a long, defined-length, N-terminal Pr

153 lamellar carcinoma (FLC) is characterized by in-frame fusion of DnaJ heat shock protein family (Hsp40

154 tion with ASOs or siRNA was achieved via the in-frame fusion of either Saccharomyces cerevisiae GAL4

155 In-frame fusion of green fluorescent protein (GFP) to th

156 Here we show that in-frame fusion of human C-propeptide of alpha1(I) colla

157 The resultant in-frame fusion protein AML1-ETO (AE) acts as an initiat

158 vidence that BCAM-AKT2 is translated into an in-frame fusion protein in the patient's tumor.

159 st of these fusion transcripts do not encode in-frame fusion proteins.

160 In-frame fusion transcripts involving histone methyltran

161 Sixty-seven in-frame fusion transcripts were identified, including t

162 This in-frame fusion was observed in 3/89 tested tumors and 2

163 tumors from 49 patients by RNA-Seq and found in-frame fusions or C-terminal truncations of MAP3K8 in

164 Our approach identified three in-frame gene fusions (YAP1-MAML2, PTPLB-RSRC1, and SP3-

165 atic mutations and unravels the landscape of in-frame gene fusions in glioblastoma.

166 ccur at two intragenic positions, leading to in-frame gene-gene fusions that generate chimeric mRNAs.

167 genome sequencing strategy and identified an in-frame germline compound heterozygous deletion, p.[Gln

168 The C-terminal in-frame green fluorescent protein fusion may slow down

169 agglutinin (IE3-HA) virus by insertion of an in-frame HA epitope sequence allowed detection of the IE

170 In particular, two individuals shared an in-frame heterozygous deletion of three nucleotides (c.4

171 erial expression vector to produce a genetic in-frame His-tagged TAT-SOD fusion protein.

172 human brain, cataloguing 123 high-confidence in-frame human NRXN1alpha isoforms.

173 Such a shortlist will aid in framing hypotheses to prioritize a manageable number

174 All four in-frame IFNGR2 hypomorphic mutant alleles encoding surf

175 ing activity and are tolerated when inserted in-frame in variable protein regions.

176 Exome analyses revealed a homozygous in-frame indel mutation (c.495_507delinsT [p.Glu165_Pro1

177 S520F, p.R1158H, p.E1163K, p.D1165H, and the in-frame indel p.VYEEDM1161V) identified in Sezary Syndr

178 ring variants defined as missense plus LoFs, in frame indels and stop-loss variants (n = 13 014, OR =

179 used to generate large libraries with random in-frame InDels across the entire single-chain variable

180 ious single-nucleotide polymorphisms (SNPs), in-frame indels, and deletions; 2) single/multigene dele

181 rate libraries of random variants with short in-frame InDels, and screen TRIAD libraries to evolve a

182 function variants 22 missense variants and 2 in-frame indels.

183 ypes (in-frame [missense point mutations and in-frame indels] versus truncating [nonsense mutations a

184 show that a naturally occurring, downstream, in-frame initiation codon is used to make a dCREB2 prote

185 on, GR(369-), which eliminates all potential in-frame initiation codons upstream of the DNA binding d

186 , instead, force translation from downstream in-frame initiation codons, yielding amino-terminally tr

187 nce located just proximal to the alternative in-frame initiation site.

188 ancestry were homozygous for an 18 base pair in-frame insertion in TKT.

189 nalysis of one splice site variant showed an in-frame insertion of 12 bp.

190 site variant (c.636+1G>A) that results in an in-frame insertion of 45 nucleotides and a missense vari

191 of 13 or more lysines followed by either an in-frame insertion of a repeat of the four amino acids p

192 predicted to cause an early truncation or an in-frame insertion or deletion.

193 associated with ADCME by identifying a novel in-frame insertion/deletion in 2 Italian families.

194 lies, we identified heterozygous missense or in-frame insertion/deletion mutations in C1R (15 familie

195 somatic hypermutation (SHM) frequencies and in-frame insertions and deletions (indels).

196 menable to structure-function analyses using in-frame insertions and deletions as presented herein.

197 n unusual DNA repeat structure that predicts in-frame insertions and deletions.

198 made it amenable to a genetic analysis using in-frame insertions and deletions.

199 d single nucleotide polymorphisms, and short in-frame insertions and deletions.

200 or specific driver events, including hotspot in-frame insertions that target KBTBD4 and 'enhancer hij

201 t SPINE-generated libraries are enriched for in-frame insertions, have drastically reduced sequence b

202 re likely to have a regulatory role, several in-frame internal ORFs within existing ORFs, resulting i

203 mutated bMSTN gene (bMSTN-mut) was inserted in frame into the pEF1a-IRES-DsRed-Express2 vector and t

204 table fusion candidates, we focused on three in-frame kinase fusion transcripts that retain a kinase

205 nt anti-ERbeta antibodies, we showed that an in-frame ligand binding domain and C terminus were prese

206 The fusion resulted in an in-frame linkage of the PRKCA catalytic domain with the

207 MCI 'non-progressors' is a weakness inherent in framing MCI primarily within a deterministic neurodeg

208 nitiates translation at codon 54, the second in-frame methionine of the TDP2 coding sequence.

209 essive versus dominant) and molecular types (in-frame [missense point mutations and in-frame indels]

210 n an experimental parameter regime for which in-frame motion prevents SPT and tight confinement of fa

211 The unique in-frame MPP5-FAM71D fusion product is important for pro

212 Two gene fusions were in-frame: MPP5-FAM71D in PC346C and ARHGEF3-C8ORF38 in G

213 s from the mutated transcript can produce an in-frame mRNA and a truncated, but still functional, pro

214 entify a homozygous nonsense mutation and an in-frame multiexon deletion in two families.

215 that the presence of at least 1 missense or in-frame mutation is associated with adult onset and slo

216 spot" regions would introduce biases towards in-frame mutations and would compromise the reproducibil

217 Kctd10, resulted in null-like phenotypes and in-frame mutations in alleles.

218 Heterozygous in-frame mutations in coding regions of human STAT3 unde

219 Here we report noncysteine in-frame mutations in IL7R and CRLF2 located in a region

220 BMD is caused by in-frame mutations in the gene encoding dystrophin, a st

221 may be obscured by proportional presence of in-frame mutations that still produce functional protein

222 the US1 gene that encodes ICP22, produces an in-frame, N-terminally truncated form of ICP22, known as

223 suggest that this classification is helpful in framing new diagnostic and therapeutic approaches to

224 in which a protein encoded by a gene with an in-frame nonsense codon at an essential lysine can be ex

225 ible gene-targeting system by introducing an in-frame nonsense mutation into the coding sequence of C

226 oding a putative TBC1D20-ZFN protein with an in-frame p.[H140_Y143del] deletion within the highly con

227 ulting in difficulty in placing the patients in frame, patient's inability or unwillingness to tolera

228 se mutations in the CHM gene resulting in an in-frame premature termination codon (PTC).

229 In-frame premature termination codons (PTCs) account for

230 Splicing regulation was most divergent in frame-preserving events and events in noncoding regio

231 In vitro, we detected expression of an in-frame protein (C-terminal PB1-F2) from downstream ATG

232 We identified at least one in-frame protein kinase fusion in 324 of 4366 samples (7

233 retrocopies often lead to the origination of in-frame proteins relative to the parental genes.

234 As (ACE-tRNA) which can effectively suppress in-frame PTCs and faithfully encode their cognate amino

235 inants occurring at gene junctions presented in-frame sequence duplications, whereas most intragenic

236 RNA sequencing identified a recurrent in-frame SH3PXD2A-HTRA1 fusion in 12/125 (10%) cases, an

237 the latter identified by mutations resulting in frame shift or premature truncation.

238 these gene structure rearrangements resulted in frame-shift mutations and premature termination codon

239 s in replicating eukaryotic cells, resulting in frame-shifting insertion/deletion (indel) mutations a

240 rom mice and humans, representing over 4,600 in-frame single-cell-derived TCRalphabeta sequence pairs

241 exon 12 (E12) as a model system because its in-frame skipping leads to a potentially functional prot

242 ce-donor-site mutation (c.730+4A>G), causing in-frame skipping of exon 8.

243 putative LoF variants susceptible to induce in-frame splicing modifications.

244 The exonic mutation c.3538G>A causes 3 in-frame splicing variants (23del, 26del, and 23/26del)

245 TgBCP1 has three potential in frame start codons that produce 51, 33 or 25 kDa prot

246 Rabies virus (RABV) P gene mRNA encodes five in-frame start codons, resulting in expression of full-l

247 duce truncated polypeptides from downstream, in-frame start codons.

248 tivated by promoter defects, introduction of in-frame stop codon, or the lack of a polyadenylation si

249 am of the LRR-encoding exons and contains an in-frame stop codon, the alternative transcript is predi

250 r the end of messenger RNA (mRNA) without an in-frame stop codon.

251 Despite the two in-frame stop codons introduced by splicing between exon

252 through across two evolutionarily conserved, in-frame stop codons of MTCH2 using luminescence- and fl

253 with genomic (main) ORF1 by converting three in-frame stop codons to nonstop codons, a uORF-ORF1 fusi

254 rosophila lebanonensis, but it does not have in-frame stop codons, suggesting the exon's functional d

255 h proteins from mutated genes with premature in-frame stop codons.

256 automated genome engineering, we introduced in-frame TAG codons into 22 essential genes, linking the

257 This approach allowed for highly efficient in-frame targeting of MYF5 in human iPS cells.

258 n FA1090 in which all opa genes were deleted in frame, termed Opaless.

259 In-frame TGA (opal) codons are found in most genes (85%)

260 ivity in vivo from a lacZ gene containing an in-frame TGA codon.

261 at expresses human calreticulin (hCRT) fused in frame to MmuPV1 E6 (mE6) and mE7 early proteins and r

262 d driven by the hly promoter (h30) or linked in frame to the ActA N-terminal 100 amino acids and driv

263 l region of the glutamate-rich protein fused in frame to the C-terminal region of merozoite surface p

264 ncoded green fluorescent protein (GFP) fused in frame to the internal capsid scaffold and maturation

265 m30 vaccine candidates expressing r30 linked in frame to the L. monocytogenes listeriolysin O signal

266 ments of MET fusing the kinase domain of MET in-frame to six different N-terminal partners.

267 DMD phenotype in 4 of 4 subjects despite an in-frame transcript.

268 bidopsis thaliana) AtSCS gene results in two in-frame transcripts and subsequently two proteins, that

269 ons fuse genes that are predicted to produce in-frame transcripts of SIRPG-WWOX, SMOC2-PROX1, and PIE

270 In the K-12 leader sequence, two in-frame translation initiation codons have been identif

271 frame in the Wnt16a mRNA to an alternative, in-frame translation initiation site, resulting in the p

272 has a one-nucleotide insertion that allowed in-frame translation of a full Cav1.2 channel.

273 of the functional complex required internal in-frame translation of small subunits from within the l

274 set of BAC-expressing neurons results in the in-frame translation of the reporter protein hence the s

275 Certain in-frame transposon insertion mutants did not interact w

276 When this gene is in-frame, Trbv5 evades TCRbeta-signaled feedback inhibit

277 xon skipping by testing whether an internal, in-frame truncation of a transmembrane protein gamma-sar

278 ded E. coli strain that lacks any endogenous in-frame UAGN sequences and release factor 1.

279 arly sensitive to antibiotics due to its ten in frame UGA codons.

280 roteinogenic amino acid encoded by a recoded in-frame UGA codon that does not operate as the canonica

281 ins occurs by translational recoding whereby in-frame UGA codons are redefined to encode the selenium

282 ), which in vertebrates may contain up to 22 in-frame UGA codons.

283 selenocysteine (Sec), which is encoded by an in-frame UGA stop codon.

284 date genes, but this approach often produces in-frame variants that retain functionality, which can o

285 , unessential, and/or functionally undefined in-frame versions of A/A*-like proteins.

286 rgely nonproductive kappa and enrichment for in-frame VJ of the others.

287 as nonproductive VJ, sterile transcripts, or in-frame VJ whose products may not associate with the H

288 mplementary DNA from renal tissue and cloned in frame with a CPD (YARKARRQARR) at the amino-terminal

289 he mixed lineage leukemia (MLL) gene fuse it in frame with multiple partner genes creating novel fusi

290 ng the extra-cellular domain of a CAR-target in frame with one of the marine luciferases or their eng

291 molog of the molecular chaperone DNAJ, fused in frame with PRKACA, the catalytic domain of protein ki

292 on of viral transcripts, fusing the pipo ORF in frame with the 5' third of the polyprotein ORF.

293 repeat and exogenous insert were positioned in frame with the native protein-encoding sequences but

294 ould produce transcripts bringing these ORFs in frame with the upstream polyprotein, thus leading to

295 heterologous proteins (ABL1, Rev, PKIA, APC) in-frame with AF10 are sufficient to immortalize murine

296 We introduced a hemagglutinin tag in-frame with Alt-ATXN1 in ATXN1 cDNA and showed in cell

297 A FusionRed (FRed) cassette was inserted in-frame with the C terminus of CSF1R, separated by a T2

298 in vivo, we introduced a luciferase reporter in-frame with the C-terminus of the dystrophin gene in m

299 ion of T-DNA into the targeted exon sequence in-frame with the hptII gene would allow hptII expressio

300 d upon either fusion of the reporter protein in frame within nonstructural protein 3 (nsP3) or insert