ライフサイエンスコーパス: induced sputum

1 y biomarkers (eosinophils and neutrophils in induced sputum).

2 lytA rtPCR was also performed on induced sputum.

3 and these cytokines were rarely detected in induced sputum.

4 n that can be demonstrated by examination of induced sputum.

5 Secreted mucin was measured in induced sputum.

6 re (CRQ), incremental shuttle walk test, and induced sputum.

7 holine, and the percentage of eosinophils in induced sputum.

8 o a comparable sample set in humans; namely, induced sputum.

9 ariable most associated with high quality of induced sputum.

10 t tomography, spirometry, and examination of induced sputum.

11 four phenotypes according to cell counts in induced sputum.

12 EPX levels and the eosinophil percentage of induced sputum.

13 on paired NPSs and NPAs; or paired NPSs and induced sputum.

14 itive on NPS, and 2 (18.2%) only positive on induced sputum.

15 sis therapy was 2% (1-3) for expectorated or induced sputum.

16 ave been accurately identified in 120 muL of induced sputum.

17 as percentage change in neutrophil counts in induced sputum 6 h after lipopolysaccharide challenge.

18 Induced sputum allows to measure mediators of asthmatic

19 pared to PCR, culture sensitivity was 60% in induced sputum and 40% in NPSs.

20 9 (6.5%) were bacteriologically confirmed on induced sputum and 72 (16.1%) were bacteriologically con

21 also analyzed the nicotine concentration in induced sputum and BAL.

22 hosphate (AMP) (differential) cell counts in induced sputum and blood and corticosteroid treatment re

23 ere identified through the use of culture of induced sputum and blood, urinary antigen detection, pol

24 Indeed, high levels of IL-17 are found in induced sputum and bronchial biopsies obtained from pati

25 Outcomes included inflammatory cells in induced sputum and bronchial biopsies, and methacholine

26 e, we compare the utility of lytA rtPCR from induced sputum and from NP swabs.

27 cterial microbiota profiles were analyzed in induced sputum and oral wash samples from 32 subjects wi

28 Induced sputum and peripheral blood obtained after each

29 Induced sputum and peripheral blood were obtained at bas

30 Induced sputum and plasma were collected prospectively f

31 Single induced sputum and stool specimens were subjected to cul

32 itutions have little diagnostic success with induced sputum, and BAL is invasive and expensive.

33 etry the CCR4-expressing T cells from blood, induced sputum, and biopsy samples of patients with asth

34 onses to experimental RV infection in blood, induced sputum, and bronchial lavage in 10 asthmatic and

35 in samples of bronchoalveolar-lavage fluid, induced sputum, and bronchial-biopsy specimens obtained

36 y, by radiography, by pulmonary function, by induced sputum, and by histological analysis.

37 Among them, deep throat saliva and induced sputum are desired for RT-qPCR test or other ear

38 in young children, gastric aspirate (GA) and induced sputum, are invasive and rarely collected in res

39 erall, lytA rtPCR performs similarly well on induced sputum as on NP swabs for most patients but perf

40 With "bacteriologically confirmed on induced sputum" as a reference, the sensitivity and spec

41 the analysis of inflammatory cell counts in induced sputum, asthmatic patients can be classified int

42 Both groups underwent spirometry and induced sputum at baseline and at 1, 3, and 6 wk.

43 e percentage of columnar epithelial cells in induced sputum at baseline was associated with the sever

44 crease of eosinophils, monocytes, and ECP in induced sputum at V3 compared with V1.

45 ul in all children who had no eosinophils in induced sputum before the attempted reduction.

46 opharyngeal/oropharyngeal (NP/OP) swabs, and induced sputum by culture and PCR.

47 Bacterial detection in induced sputum by PCR decreased 7% for exposed compared

48 d nasopharyngeal and oropharyngeal swabs and induced sputum (cases only) for Bordetella pertussis pol

49 Induced sputum CC16 and SPD were significantly higher in

50 According to induced sputum cell count, four different asthma phenoty

51 Effects on induced sputum cell counts and differentials, eosinophil

52 Participants had induced sputum cell counts measured and blood analysed f

53 Exhaled nitric oxide (eNO), induced sputum cell counts, and responsiveness to methac

54 timated because of the exclusive reliance on induced sputum cell counts, which are variable among coh

55 In induced sputum cell pellets from 37 asthmatic patients a

56 tion of various proinflammatory mediators in induced sputum cell-free supernatant in eight patients w

57 Induced sputum cells and fractional exhaled nitric oxide

58 gene expression (type 2 gene mean [T2GM]) in induced sputum cells from healthy control subjects, pati

59 ion, we investigated FPR2/ALXR expression in induced sputum cells obtained from the study groups.

60 FPR2/ALXR expression was reduced in induced sputum cells of children with SA compared with t

61 parable in each group, whereas the amount of induced sputum collected from the CF producers was over

62 ch to October 2015, 320 infants had NPSs and induced sputum collected, and 11 (3.4%) pertussis cases

63 lowed by methacholine challenge (day 7), and induced sputum collection and fractional exhaled nitric

64 adrupole time-of-flight mass spectrometry in induced sputum comparing smokers with and without COPD,

65 We conclude that induced sputum contains high concentrations of eicosanoi

66 nd approximately 20% reduction in yield from induced sputum culture.

67 ected South African adults if blood culture, induced-sputum culture or Gram stain, urine antigen test

68 These results provide direct evidence that induced sputum derives from the central airways with lit

69 Indirect evidence suggests that induced sputum derives from the surfaces of the bronchia

70 nophilic airway inflammation was assessed by induced sputum differential eosinophil count.

71 An induced sputum differential inflammatory cell count sugg

72 Induced sputum differentials were obtained.

73 essment and immunological sampling of blood, induced sputum, endobronchial biopsy, and bronchoalveola

74 nt detailed clinical assessment and provided induced sputum, endobronchial biopsy, bronchoalveolar la

75 Serum and induced sputum endotoxin and cytokine concentrations wer

76 management group) or by normalisation of the induced sputum eosinophil count and reduction of symptom

77 stically significant attenuation of allergen-induced sputum eosinophil count on Day 4 following GSK21

78 nt strategy directed at normalisation of the induced sputum eosinophil count reduces asthma exacerbat

79 ol of eosinophilic inflammation, measured by induced sputum eosinophil count.

80 utum eosinophil peroxidase (EPX) levels with induced sputum eosinophil percentage in 10 adults with p

81 al EPX and pharyngeal EPX levels compared to induced sputum eosinophil percentage were 0.81 (P = 0.00

82 was associated with attenuation of allergen-induced sputum eosinophilia and with lower levels of try

83 and 75 mug significantly attenuated allergen-induced sputum eosinophilia by 63 and 61% at 7 hours, re

84 Budesonide did not reduce allergen-induced sputum eosinophilia versus placebo.

85 The level of induced sputum eosinophilic cationic protein mug/l, was

86 Test), spirometry, exhaled nitric oxide and induced sputum evaluation.

87 ry and present the clinical, radiologic, and induced sputum features.

88 andin (PG)D2 (PGDM) and leukotriene (LT)E4 , induced sputum fluid LTB4 , LTE4 , PGD2 , and PGE2 , pla

89 We collected blood for culture, induced sputum for culture and multiplex polymerase chai

90 Methods: We measured sputum eDNA in induced sputum from 399 patients with asthma in the Seve

91 Analysis of induced sputum from a subset of SARP subjects showed 4 s

92 onally, OSM protein levels were increased in induced sputum from asthmatic patients compared with tha

93 IL-32 is detectable in induced sputum from asthmatic patients.

94 Whole genome sequencing was performed on induced sputum from non-smoking (SAn) and current or ex-

95 cytokine analysis (ELISAs) were performed on induced sputum from T2-high severe asthmatic patients in

96 y of SECs may be a useful quality measure of induced sputum from young children with pneumonia.

97 We evaluated induced sputum Gram stain smears and cultures from hospi

98 Compared with control, induced sputum greatly enhanced clearance after central

99 Induced sputum has been used to study airway inflammatio

100 Induced sputum has provided considerable information abo

101 tion, such as exhaled nitric oxide (eNO) and induced sputum, has been shown to improve asthma monitor

102 (NPSs), nasopharyngeal aspirates (NPAs), and induced sputum, have been used for B. pertussis detectio

103 d children as bacteriologically confirmed on induced sputum if any test performed on induced sputum w

104 o characterize and compare the microbiome of induced sputum in asthmatic and nonasthmatic adults.

105 Protocols for induced sputum in children were collated from published

106 chest radiograph interpretation, utility of induced sputum in children, measurement of pathogen dens

107 We induced sputum in hay fever patients and healthy control

108 n and the reproducibility of measurements in induced sputum in multicenter studies is unknown.

109 Induced sputum inflammatory cells, neutrophils, interleu

110 philic airway inflammation measured by using induced sputum is an important treatment stratification

111 at measurement of markers of inflammation in induced sputum is as reproducible as methacholine PC(20)

112 Induced sputum is indeed not widely available and marker

113 Induced sputum is occasionally used to investigate lower

114 Induced sputum (IS) allows to measure mediators of asthm

115 were enrolled.Measurements and Main Results: Induced sputum (IS) and nasopharyngeal aspirates (NPAs)

116 uberculosis/rifampicin) automated testing on induced sputum (IS) is possible, but the capacity for pe

117 Induced sputum (IS) may provide diagnostic information a

118 The incremental value of a repeated induced sputum (IS) sample, compared with a single IS or

119 ction of respiratory viruses and bacteria in induced sputum (IS) specimens from children hospitalized

120 Induced sputum (IS) was collected from patients with NER

121 Oral swabs and induced sputum (IS) were collected from 201 South Africa

122 o hundred thirty-two clinical specimens (120 induced-sputum [IS] and 112 bronchoalveolar lavage [BAL]

123 risk, and we confirmed our findings by using induced sputum macrophages isolated from healthy human v

124 findings were confirmed by using DEP-exposed induced sputum macrophages isolated from healthy volunte

125 the relationship of efferocytosis by airway (induced sputum) macrophages and blood monocytes to marke

126 We conclude that markers in induced sputum may be useful, noninvasive outcome measur

127 rmed cross-sectional analyses of the airway (induced sputum) microbiome and cytokine profiles from bl

128 We evaluated the diagnostic utility of induced sputum microscopy and culture in patients enroll

129 Induced sputum microscopy and culture results were not a

130 Plasma PIIINP correlated with induced sputum MMP-1 concentrations and radiological sco

131 cts, smokers and never smokers), we measured induced sputum MMP-12 concentrations (ELISA) and enzyme

132 ng function and computed tomography (CT) and induced sputum MMP-12 concentrations and activity in pat

133 In patients with TB, induced sputum MT1-MMP mRNA levels were increased 5.1-fo

134 ed TB who provided expectorated (n = 438) or induced sputum (n = 128), tracheal aspirates (n = 71), b

135 expression was assessed on the total RNA of induced sputum (n = 83), nasal brushings (n = 41), and e

136 tra; Cepheid), a new rapid test, on repeated induced sputum, nasopharyngeal aspirates, and combinatio

137 tudy generated gene expression profiles from induced sputum of 47 asthmatic patients.

138 soluble IL-6 receptor (sIL-6R) expression in induced sputum of asthmatic patients was correlated with

139 l propeptide (PIIINP) was 3.8-fold higher in induced sputum of HIV-uninfected tuberculosis patients c

140 coinfection decreased MMP concentrations in induced sputum of patients with TB.

141 An induced sputum or an endotracheal aspirate was obtained

142 inflammatory processes and biomarkers using induced sputum or bronchial biopsies.

143 ly involve invasive sampling methods such as induced sputum or bronchoalveolar lavage that are uncomf

144 agnosed by direct microscopic examination of induced sputum or by bronchoalveolar lavage (BAL).

145 n separate occasions followed immediately by induced sputum or no sputum (control), while seated in f

146 Whether microbiome characteristics of induced sputum or oral samples demonstrate unique relati

147 using multicopy gene targets, together with induced sputum or oral wash samples.

148 ed if M. tuberculosis was detected on either induced sputum or stool.

149 EVs were isolated from induced sputum or supernatants of cultured nasal polyp o

150 Sputum, induced sputum, or bronchial specimens are all suitable

151 pneumonia" (nasopharyngeal/oropharyngeal or induced sputum PCR-positive without confirmed/suspected

152 ryngeal swabs, nasopharyngeal aspirates, and induced sputum performed similarly for the detection of

153 (PERCH) study, we reviewed the literature on induced sputum procedures.

154 qPCR on induced sputum provided similarly high sensitivity of 99

155 with >/=2 TB-related symptoms, obtained one induced sputum sample for smear microscopy (AFB) and myc

156 sts including chest radiography and repeated induced sputum sample testing with Xpert MTB/RIF and liq

157 One induced sputum sample was available for analysis from 37

158 ificantly more radioactivity recovered in an induced sputum sample, and greater airways clearance of

159 ), respectively, with use of expectorated or induced sputum samples and 66% (51-81) and 98% (96-99),

160 Furthermore, induced sputum samples are similar to spontaneously expe

161 llenge from patients with mild asthma and in induced sputum samples collected from patients with mild

162 he direct detection of CFP32 by ELISA in the induced sputum samples from 56% of pulmonary TB patients

163 Methods: We collected induced sputum samples from a cohort of nonsmokers, smok

164 tion analysis on cells from nasal lavage and induced sputum samples from all subjects after RV16 inoc

165 Comparisons were made between induced sputum samples from hospitalized children with r

166 ed reduced chemotaxis (approximately 30%) to induced sputum samples obtained during theophylline trea

167 atric asthma using flow cytometry to analyse induced sputum samples obtained from healthy and asthmat

168 IL-32 was determined in serum and induced sputum samples of asthmatic patients and healthy

169 t surprisingly, the microbiome data from the induced sputum samples we collected revealed a significa

170 Induced sputum samples were analysed using 7-colour flow

171 oke exposure (500 mug/m(3)) for 2 hours, and induced sputum samples were collected and processed for

172 Induced sputum samples were obtained from 10 nonasthmati

173 r the detection of pulmonary tuberculosis on induced sputum samples, using mycobacterial cultures as

174 iral therapy, many centers no longer collect induced sputum samples.

175 3%-76%) had positive test results on up to 2 induced sputum samples.

176 Results show that sphingolipid expression in induced sputum significantly differs between smokers wit

177 ercentage of neutrophils in sputum (%PMN) in induced sputum similarly and significantly increased in

178 sis of B. pertussis culture was performed on induced sputum specimens and in a subset of NPSs.

179 valence of potential pathogens cultured from induced sputum specimens and quantity of oropharyngeal f

180 of this study do not support the culture of induced sputum specimens as a diagnostic tool for pneumo

181 The microbial composition of induced sputum specimens collected from adult patients s

182 The microbial composition of induced sputum specimens collected from asthma patients

183 Only 26.3% of 99 induced sputum specimens collected were determined to be

184 t clinics were very proficient at collecting induced sputum specimens for the diagnosis of Pneumocyst

185 These same indices in the induced sputum specimens from CF producers were signific

186 uality ratings have never been evaluated for induced sputum specimens from children with suspected pn

187 Two induced sputum specimens were tested with Xpert MTB/RIF

188 Of 3772 induced sputum specimens, 2608 (69%) had <10 SECs per lo

189 putum cells, as well as eicosanoid levels in induced sputum supernatant (ISS) and urine were extracte

190 ctrometry was used to profile eicosanoids in induced sputum supernatant (ISS).

191 Induced sputum supernatant and plasma was obtained befor

192 We have measured induced sputum supernatant LTC(4)/D(4)/E(4) concentratio

193 ctional analysis of the lipid composition of induced sputum supernatant obtained from asthma patients

194 Shotgun lipidomics of induced sputum supernatant revealed a spectrum of 9 mole

195 Induced sputum supernatant was collected from 211 adults

196 ous proinflammatory and tussive mediators in induced sputum supernatants from 20 patients with cough

197 We examined LXA4 and LTB4 concentrations in induced sputum supernatants from children with intermitt

198 Chemotaxis experiments were performed using induced sputum supernatants from subjects with and witho

199 ith SA have decreased LXA4 concentrations in induced sputum supernatants in comparison with children

200 sues and cell types (i.e. epithelial, blood, induced sputum, T cells and lymphoblastoids).

201 175 oral washes, each paired with either an induced sputum that was positive for Pneumocystis or a B

202 iratory specimen types (expectorated sputum, induced sputum, tracheal aspirates, bronchoalveolar lava

203 spected pneumonia, from whom expectorated or induced sputum, tracheal secretions, or bronchoalveolar

204 on of candidate SNPs with mRNA expression in induced sputum was also elucidated.

205 Induced sputum was analyzed for eosinophilic inflammatio

206 Induced sputum was assayed for bacterial density, cell c

207 Induced sputum was assessed for neutrophils 4 hours afte

208 Induced sputum was collected at baseline before rhinovir

209 Induced sputum was collected before and following bronch

210 Induced sputum was collected before and repeatedly after

211 d to control for inhaled corticosteroid use, induced sputum was collected.

212 ncreased integrin beta3 mRNA accumulation in induced sputum was detected, to our knowledge, for the f

213 de-induced inflammatory cell infiltration in induced sputum was investigated in 21 healthy men.

214 Induced sputum was obtained 3.5 h after exposure.

215 Induced sputum was obtained before and 48h after a whole

216 Induced sputum was obtained for simultaneous enrichment

217 Induced sputum was obtained from nonsmoking patients wit

218 Induced sputum was obtained, and airway hyperresponsiven

219 d on induced sputum if any test performed on induced sputum was positive for Mycobacterium tuberculos

220 Induced sputum was sampled 24 hours after exposure.

221 Induced sputum was used to measure mast cell mediators a

222 Induced sputum weights were comparable in each group, wh

223 her markers of infection and inflammation in induced sputum were a useful and safe outcome measure in

224 , baseline FEV(1) and percent eosinophils in induced sputum were not significantly associated with LA

225 biopsy specimens, epithelial brushings, and induced sputum were obtained from asthmatic patients wit

226 mptom screening, chest radiography (CXR) and induced sputum were performed.

227 Nasal secretions and induced sputum were repeatedly sampled over the next 14

228 Bronchial biopsies and induced sputum were taken from 140 current and former sm

229 Nasopharyngeal specimens and induced sputum were tested by polymerase chain reaction

230 n 149 (67.1%) of 222 patients with available induced sputum, whereas the results of either Gram stain

231 t in asthmatic airway secretions (sampled as induced sputum), which possess chemotactic activity for

232 eproducibility of markers of inflammation in induced sputum with the reproducibility of the FEV(1) an

233 acterial density and inflammatory content of induced sputum would decrease after antibiotic therapy.