ライフサイエンスコーパス: inducible NOS

1 ammatory mediators, and expression of COX-2, inducible NOS, and NADPH oxidase.

2 gulation of mRNAs encoding cyclooxygenase-2, inducible NOS, IL-6, and IL-1beta but blunted the produc

3 In noninfarcted myocardium, although inducible NOS (iNOS) mRNA was absent, TNF-alpha, IL-1bet

4 Induction of mRNA for an inducible NOS (iNOS) in DC was confirmed by Northern blo

5 We analyzed inducible NOS (iNOS) expression in ventricular myocardiu

6 eNOS) and inflammation (cyclooxygenase-2 and inducible NOS) and markers of oxidative stress (nitrotyr

7 sion of nitrotyrosine, cyclooxygenase-2, and inducible NOS significantly decreased in patients who ad

8 In addition, constitutive NOS (cNOS) and inducible NOS (iNOS) immunostaining were used for coloca

9 uorescent staining revealed constitutive and inducible NOS in human oviduct and cumulus (the cellular

10 abrogated by inhibitors of constitutive and inducible NOS.

11 he relative contributions of endothelial and inducible NOS (eNOS and iNOS, respectively) to angiogene

12 ich the reductase domains of endothelial and inducible NOS, respectively, were replaced by the reduct

13 tivities, immune cell infiltration, eNOS and inducible NOS (iNOS) expression, sphingosine 1 phosphate

14 s in the expression of eNOS [eNOS (-/-)] and inducible NOS [iNOS (-/-)] was measured with a Clark-typ

15 g enzymes endothelial NO synthase (eNOS) and inducible NOS (iNOS) in human bladder cancer.

16 t purified nNOS, endothelial NOS (eNOS), and inducible NOS (iNOS) enzymes.

17 ed nNOS enzymes, endothelial NOS (eNOS), and inducible NOS (iNOS).

18 onal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS).

19 und that several flavones inhibit Muc5AC and inducible NOS up-regulation as well as cytokine release

20 hibitor L-NAME and in mice with neuronal and inducible NOS deficiency, suggesting that excessive nitr

21 These results suggest that nNOS and inducible NOS but not endothelial NOS are irreversibly i

22 deficient in neuronal NO synthase (nNOS) and inducible NOS (iNOS) resisted apoptosis.

23 nal experiments with neuronal NOS (nNOS) and inducible NOS (iNOS) variants (nNOS W409F and iNOS K82A

24 thelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS) in cardiovascular regulation.

25 thelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS).

26 ectivities for nNOS over endothelial NOS and inducible NOS of the most potent nNOS inhibitor (10a) am

27 synthase (NOS) isoforms (endothelial NOS and inducible NOS).

28 polyclonal antibodies to endothelial NOS and inducible NOS, revealed a diffuse pattern of positive la

29 levels of endothelial NO synthase (NOS) and inducible NOS were increased by HIV-1 Tat stimulation.

30 cal role: neuronal NOS, endothelial NOS, and inducible NOS (iNOS).

31 ong-lasting reduction of blood perfusion and inducible NOS expression selectively in the ileum but no

32 erized by an increased production of TNF and inducible NOS (iNOS) in the lungs.

33 and with antibodies against constitutive and inducible NOSs.

34 but not anti-endothelial NOS (eNOS) or anti-inducible NOS (iNOS) antibodies, whereas skeletal muscle

35 Tadalafil rapidly augmented inducible NOS (iNOS) expression by increasing its mRNA,

36 Because inducible NOS is induced after bile duct ligation but no

37 NO generated by inducible NOS (iNOS) causes buildup of S-nitrosated GAPD

38 This is mediated by inducible NOS, which is expressed in the aging endotheli

39 in-mediated MKP-1 expression was preceded by inducible NOS (iNOS) induction and cGMP production.

40 ially through modulation of NO production by inducible NOS or constitutive NOS.

41 nitric oxide synthase 2 (NOS2), often called inducible NOS, plays a central role in the inflammatory

42 depressed in congestive heart failure (CHF), inducible NOS (iNOS) may be expressed in failing myocard

43 By contrast, under inflammatory conditions, inducible NOS (iNOS)-expressing CD11b(+)Gr-1(+) cells at

44 riggers nuclear factor kappaB and consequent inducible NOS (iNOS)-mediated overproduction of NO, whic

45 results identify the cell types contributing inducible NOS (iNOS) to experimental wounds in rats.

46 Conversely, inducible NOS (iNOS) protein expression was not signific

47 onds to G-CSF by engaging Cathepsin S-CX3CR1-inducible NOS signaling.

48 m of nitric oxide (NO) synthase (NOS), i.e., inducible NOS (iNOS), thereby inducing NO, which in turn

49 vation injury and to characterize endogenous inducible NOS (iNOS) expression.

50 Endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), nitrotyrosine, and tissue hypoxia

51 caveolin-2, resulted in inhibition of eNOS, inducible NOS (iNOS), and neuronal NOS (nNOS) activities

52 The first inducible NOS to be described from an invertebrate regul

53 Here, we characterized a fluorescent inducible NOS (iNOS) inhibitor called PIF (pyrimidine im

54 ild-type mice and those lacking the gene for inducible NOS (iNOS).

55 , a novel and highly selective inhibitor for inducible NOS (iNOS), on in vivo growth of solid tumors

56 ooth muscle cells immunostained strongly for inducible NOS and nitrotyrosine residues.

57 Here, we characterize pterin-free inducible NOS (iNOS) and iNOS reconstituted with eight d

58 increased NO production did not result from inducible NOS expression.

59 However, inducible NOS (iNOS) was increased after PDT in macropha

60 ] (FMNH(*): FMN semiquinone) form of a human inducible NOS (iNOS) bidomain oxygenase/FMN construct.

61 d 48.7% sequence identity to mouse and human inducible NOS and to two constitutive NOSs from rat brai

62 aracterize the activity of recombinant human inducible NOS (iNOS).

63 ere, we extend this methodology to the human inducible NOS (iNOS) isoform, comparing wild-type (wt) a

64 The human inducible NOS (iNOS) promoter transcriptionally regulate

65 NOS-II (inducible NOS) mRNA expression was analyzed 2, 4, 6 and

66 se 2 (NOS2) mRNA in their lungs, implicating inducible NOS as a defense mechanism in this disease.

67 ultures of microglia and preOLs deficient in inducible NOS (iNOS) or gp91(phox), the catalytic subuni

68 ion of NF-kappaB accompanied by increases in inducible NOS, macrophage inflammatory protein-2 (MIP-2)

69 ide synthase (eNOS) uncoupling and increased inducible NOS (iNOS) activity amplify vascular oxidative

70 Because increased inducible NOS (iNOS) expression and NO generation are as

71 t IL-6 decreases contractility and increases inducible NOS (iNOS) in adult rat ventricular myocytes (

72 Ca2+-independent NOS activity, indicating inducible NOS (NOS2), is markedly expressed in approxima

73 manner, demonstrating its ability to inhibit inducible NOS activity in vivo.

74 nNOS over eNOS (endothelial NOS), and iNOS (inducible NOS) are greatly increased in these series.

75 Interestingly, inducible NOS mRNA levels showed a significant (P <0.05)

76 c-oxide synthase (nNOS) and mouse macrophage inducible NOS was inhibited by the hydrogen sulfide (H2S

77 acid C-terminal tail from murine macrophage inducible NOS (iNOS) holoenzyme and from a flavin domain

78 Macrophage-inducible NOS generates NO to kill other cells, whereas

79 S in lipopolysaccharide-treated macrophages (inducible NOS; type II NOS), and bovine pulmonary endoth

80 kilobases (kb), a size similar to mammalian inducible NOS.

81 osed IL-1beta knockout (IL-1beta(-/-)) mice, inducible NOS knockout (iNOS(-/-)) mice, and wild-type m

82 thelial NO synthase knockout (eNOS KO) mice, inducible NOS KO mice, the NO donor S-nitroso-N-acetylpe

83 down-regulates basal and cytokine-modulated inducible NOS (NOS2) expression in human cells in vitro.

84 p that interacts with H(4)B (Trp457 in mouse inducible NOS) regulates the kinetics of electron transf

85 ty of individually inactive mutants of mouse inducible NOS (iNOS; NOS2), each deficient in binding a

86 med single turnover reactions with the mouse inducible NOS oxygenase domain (iNOSoxy) mutants W457F a

87 nerated complementary mutations in the mouse inducible NOS oxygenase domain (iNOSoxy, V346I) and in b

88 Crystal structures of the mouse inducible NOS oxygenase domain reveal a homologous BH4-b

89 ponding human neuronal NOS (nNOS) and murine inducible NOS (iNOS) fusion proteins have also been expr

90 tructs of rat neuronal NOS (nNOS) and murine inducible NOS (iNOS), in which only the oxygenase and FM

91 Mutation of Trp-188 to histidine in murine inducible NOS was shown to retard NO synthesis and allow

92 man endothelial NO synthase (eNOS) or murine inducible NOS (iNOS) cDNA to study the regulatory role o

93 Structures of the murine inducible NOS oxygenase domain (iNOS(ox)) complexed with

94 xpression of NAD(P)H-oxidase, nitrotyrosine, inducible-NOS, and NF-kappaB, suggesting decreased super

95 We found dose-dependent Type II NOS [inducible NOS (iNOS)] mRNA upregulation in dura mater be

96 ole of one of the cytosolic isoforms of NOS [inducible NOS (NOSII)] in the bioactivation of this DNA-

97 RL, VIP, neural nitric oxide synthase (NOS), inducible NOS, and the interferon type I receptor were s

98 -oxide synthase (NOS) isoforms neuronal NOS, inducible NOS (iNOS), and endothelial NOS in a protein k

99 OX-2, and NOX4), nitric-oxide synthase [NOS; inducible NOS and endothelial NOS], and cyclooxygenase (

100 ing demonstrated an increase in eNOS but not inducible NOS (iNOS) expression.

101 thelial NOS (eNOS) and neuronal NOS, but not inducible NOS, were demonstrated in P and NP arteries.

102 ney tissue with large VT ventilation but not inducible NOS.

103 S100 beta can induce a potent activation of inducible NOS in astrocytes, an observation that might h

104 It is believed that the activity of inducible NOS (iNOS) is regulated primarily at the trans

105 increase was mainly due to high activity of inducible NOS, whereas eNOS activity was reduced.

106 ficient mice, but did not affect activity of inducible NOS.

107 trimeric S-nitrosylase complex consisting of inducible NOS (iNOS), S100A8, and S100A9.

108 ug also enhanced mRNA and protein content of inducible NOS (iNOS) and endothelial NOS (eNOS) in the m

109 Previous work with the oxygenase domain of inducible NOS (iNOSoxy) demonstrated that H(4)B radical

110 n-bound complexes of the oxygenase domain of inducible NOS (iNOSoxy).

111 d its subsequent effect on the expression of inducible NOS (iNOS) and nitrite biosynthesis using cell

112 aHS unexpectedly augmented the expression of inducible NOS (iNOS) but not endothelial NOS.

113 The expression of inducible NOS (iNOS) in various tissues was estimated by

114 Resting T cells had minimal expression of inducible NOS (NOS2), endothelial NOS (NOS3), and GTPCH-

115 his response was caused by the expression of inducible NOS and could be blocked by pharmacologic inhi

116 he heme domain, (c) the Ca2+ independence of inducible NOS requires interactions of calmodulin with b

117 hobic residues in the Ca(2+) independence of inducible NOS.

118 reductions in insulin-mediated induction of inducible NOS protein expression and cGMP generation but

119 y LPS of 5-LO metabolism and no induction of inducible NOS.

120 timulated astrocytes, owing to inhibition of inducible NOS (iNOS) protein expression.

121 nary ligation in situ, whereas inhibition of inducible NOS and neuronal NOS had no effect.

122 In striking contrast, a genetic lack of inducible NOS, which does not affect BP, has no effect o

123 /cJ and BALB/cByJ mice had similar levels of inducible NOS mRNA in their brains, which were not affec

124 erated and characterized the W457A mutant of inducible NOS and the corresponding W678A and W678F muta

125 inhibitors, and mice with null mutations of inducible NOS (iNOS) or eNOS.

126 rleukin (IL)-1beta by decreasing the rate of inducible NOS gene transcription.

127 comitantly with significant up-regulation of inducible NOS (iNOS) levels.

128 The exact role of inducible NOS (iNOS) in liver ischemia/reperfusion (I/R)

129 rmacological inhibition or gene silencing of inducible NOS abrogated LPS-induced LKB1 degradation, wh

130 New crystallographic structures of inducible NOS(ox) reveal that conformational changes in

131 ty of endothelial NOS was decreased, that of inducible NOS was increased, and both were preserved in

132 X inhibitors to determine the role of COX on inducible NOS (iNOS) expression in the gastric mucosa.

133 MA and N(G)-nitro-l-arginine methyl ester on inducible NOS (macrophages) and endothelial NOS in vitro

134 ffects on NF-kappaB activation and ICAM-1 or inducible NOS expression, the NOS inhibitor almost compl

135 ciated with increased neuronal NOS (nNOS) or inducible NOS (iNOS) activity in a variety of neurologic

136 produced from neuronal NO synthase (nNOS) or inducible NOS (iNOS) during inflammation of the central

137 whereas deficiency of neuronal NOS (nNOS) or inducible NOS (iNOS) suppresses choroidal, but not retin

138 Inhibition of neuronal NOS or inducible NOS did not affect baselines, or evoked respon

139 o-L-arginine) or genetic (endothelial NOS or inducible NOS) inhibition of NOSs in females enhances ki

140 y nNOS, but unaffected by endothelial NOS or inducible NOS.

141 ression of nitric oxide synthase-2 (NOS2, or inducible NOS) contributes to this loss of inotropic res

142 ion of p65 is not accompanied by TNFalpha or inducible NOS (iNOS) expression.

143 increase in isoform selectivity of nNOS over inducible NOS was not obtained in this series.

144 5, 17, 21), as well as good selectivity over inducible NOS.

145 Foxo1 promotes inducible NOS (iNOS)-dependent NO-peroxynitrite generati

146 No change in pulmonary inducible NOS levels or localization was detected.

147 ing growth factor (TGF)-beta1 down-regulates inducible NOS after its induction by interleukin (IL)-1b

148 a45eNOS exhibited characteristics resembling inducible NOS (iNOS).

149 ed nitric oxide synthase (OA-NOS) and rodent inducible NOS (iNOS).

150 ced mRNA levels for TNFalpha, CCL20, S100A7, inducible NOS, and IL-36gamma itself.

151 , pretreatment with the relatively selective inducible NOS (iNOS) inhibitor S-methylisothiourea sulfa

152 ase subunits (gp91phox and P67phox), Mn SOD, inducible NOS (iNOS), endothelial NOS (eNOS), and nitrot

153 vealed that, compared with control subjects, inducible NOS, endothelial NOS, and nitrotyrosine, but n

154 st that the ability of TGF-beta1 to suppress inducible NOS promoter/enhancer activity occurs through

155 ession of a distinct isoform of NO synthase (inducible NOS [iNOS]) contributing to the suppression of

156 An antisense targeting inducible NOS is inactive.

157 Our recent work has provided evidence that inducible NOS (iNOS) may also catalyze O-2 formation in

158 ing one-tenth and one-sixth as active as the inducible NOS (iNOS) and the neuronal NOS (nNOS), respec

159 open reading frame of the cDNA encoding the inducible NOS (iNOS) were designed to produce a selectiv

160 ibitors of, or alternate substrates for, the inducible NOS isoform.

161 ortance we substituted His for Trp188 in the inducible NOS oxygenase domain (iNOSoxy) and characteriz

162 previously that H4B radical formation in the inducible NOS oxygenase domain (iNOSoxy) is kinetically

163 ucible factor-1 (HIF-1) on expression of the inducible NOS (iNOS) in myocardial cells in vivo and in

164 r plasmids containing various lengths of the inducible NOS 5'-flanking region into primary cultured r

165 f the plasmid containing -1485 to +31 of the inducible NOS gene by more than 10-fold, indicating the

166 at a construct containing -234 to +31 of the inducible NOS gene contained the majority of promoter/en

167 Co-administration of the inducible NOS inhibitor S-methylisothiourea or the prote

168 A heme domain construct of the inducible NOS isoform (iNOSheme) was used to assess the

169 ted after cytokine induced expression of the inducible NOS isoforms.

170 odulating airway responsiveness and only the inducible NOS isoform appears to respond to antigen stim

171 Our single-catalytic turnover study with the inducible NOS oxygenase domain showed that a conserved T

172 Western blot analysis verified inducible NOS (iNOS) expression after TNFalpha exposure.

173 gly similar to that described for vertebrate inducible NOS gene promoters.

174 s with OSA than in control subjects, whereas inducible NOS expression was 56% greater.

175 olecules identified a novel quinolinone with inducible NOS (iNOS) inhibitory activity.