ライフサイエンスコーパス: initiator caspase

1 proposed, which suggests caspase-2 to be an initiator caspase.

2 strating its capacity to affect a vertebrate initiator caspase.

3 polyubiquitination and aggregation of a key initiator caspase.

4 domains is required for their processing by initiator caspases.

5 tive suppression than inhibitors of upstream initiator caspases.

6 daptor protein bridging death receptors with initiator caspases.

7 ause it induces apoptosis independent of the initiator caspases.

8 le of selectively binding to large prodomain initiator caspases.

9 are sufficient to cleave and activate other initiator caspases.

10 activity that resides in the zymogens of the initiator caspases.

11 requires a novel pathway in addition to the initiator caspases.

12 be achieved through restricted activation of initiator caspases.

13 d by mature executioner caspases rather than initiator caspases.

14 sts diverse mechanisms for the activation of initiator caspases.

15 d near complete loss of the p10 subunit from initiator caspases 1 and 8 but not from the executioner

16 effectors of pyroptosis and apoptosis, where initiator caspases-1 and -8 also functioned as execution

17 damaging agent camptothecin to activate the initiator caspase 2.

18 death was associated with activation of both initiator (caspases-2, -8 and -10) and effector (caspase

19 death is partly controlled by the apoptotic initiator caspase-2 (C2).

20 The apoptotic initiator caspase-2 has been implicated in oocyte death,

21 Here, we show that the initiator caspase-2 is required for robust death of ovar

22 ced apoptosis and affinity-labeled activated initiator caspase-2, -8, and -9.

23 a BH3-only protein that is processed by the initiator caspase-2.

24 These bile acids differentially activate initiator caspases-2 and -8 and induce cleavage of full-

25 duced c-Jun N-terminal kinase activation and initiator caspase 8 cleavage.

26 the effector caspases 3 and 7, although the initiator caspase 8 had been activated.

27 pase cascade, antagonizing the activation of initiator caspase 8.

28 eolytic cascade by recruiting and activating initiator caspases 8 and 10.

29 Activation of both initiator caspases 8 and 9 and effector caspase 3 was no

30 bisphosphate (PIP2) is a direct inhibitor of initiator caspases 8 and 9, and their common effector ca

31 (i) rapid formation of covalent complex with initiator caspases 8 or 1, (ii) very slow deacylation, a

32 y monitor executioner (caspase-3 and -7) and initiator (caspase-8 and -9) caspase activity.

33 erexpression of TIMP-3 induced activation of initiator caspase-8 and -9 and promoted caspase-mediated

34 gnals from death receptors to the downstream initiator caspase-8 and connects to the mitochondrial in

35 aspases and, surprisingly, activation of the initiator caspase-8 and processing of its substrate Bid.

36 ken together these data demonstrate that the initiator caspase-8 can directly activate pro-caspase-3

37 rigger apoptosis by recruiting the apoptosis initiator caspase-8 through the adaptor FADD.

38 platforms that either activate the apoptotic initiator Caspase-8 to trigger cell death, or signal thr

39 lustering with subsequent recruitment of the initiator caspase-8, and ultimately cellular demise.

40 We show here that in a purified system, the initiator caspases-8 and -10 directly process the execut

41 only modulated by either VES or MSA included initiator caspases-8 and -10, as well as executioner cas

42 rinsic apoptotic pathways with activation of initiator caspases-8 and -9 and downstream effector casp

43 ation of the effector caspase-3 but also the initiators caspase-8 and caspase-9, mitochondrial cytoch

44 apoptosis is the activation of the apoptotic initiator caspase 9.

45 d (1.5- and 1.4-fold, respectively), whereas initiator caspase-9 and death caspase-3 transcripts were

46 sulfides leads to enhanced persulfidation of initiator caspase-9 and decreases apoptosis.

47 mac antagonizes several IAPs and assists the initiator caspase-9 and effector caspases (caspase-3, ca

48 cytochrome c release; and (iv) activation of initiator caspase-9 and executioner caspase-3.

49 High glucose also induces loss of the initiator caspase-9 and increases caspase-3 cleavage, ef

50 sis largely through direct inhibition of the initiator caspase-9 and the effector caspase-3 and -7.

51 the executioner caspase-3 and the intrinsic initiator caspase-9 in primary cerebellar granule neuron

52 During stress-induced apoptosis, the initiator caspase-9 is activated by the Apaf-1 apoptosom

53 Activation of the initiator caspase-9 is essential for induction of apopto

54 ed in recent years, the mechanism of how the initiator caspase-9 is regulated by IAPs remains enigmat

55 D specifically cleaved caspase-8 but not the initiator caspase-9 or -10 and significantly increased c

56 la wing discs and a delayed reporter for the initiator caspase-9 ortholog Dronc activity, we identify

57 inhibiting the effectors caspase-3/-7 and an initiator caspase-9 through its BIR2 and BIR3 domains, r

58 are exemplified by XIAP, which regulates the initiator caspase-9, and the executioner caspases-3 and

59 data suggest that caspase-9 is the critical initiator caspase activated during heat-induced apoptosi

60 investigated the activation of caspase-2, an initiator caspase activated in response to DNA damage an

61 Caspase-2 is an initiator caspase activated in response to heat shock an

62 ctive caspase, we identified caspase-2 as an initiator caspase activated in rotenone-treated primary

63 Although widely regarded as an inhibitor of initiator caspase activation and cell death, c-FLIP(L) i

64 nducing signaling complex (DISC) to regulate initiator caspase activation and launch the apoptotic pr

65 evaluated and other possible mechanisms for initiator caspase activation are discussed.

66 at TNF death signals are blocked proximal to initiator caspase activation, at the level of TNF recept

67 ss, which could be explained by a failure of initiator caspase activation.

68 mitochondrial dysfunction, we determined the initiator caspase and its role in cell death in primary

69 Caspase-9 (casp-9) is an initiator caspase and plays a central role in activating

70 9 has the potential to inhibit both upstream initiator caspases and downstream effector caspases, we

71 ins assemble into the DISC to fully activate initiator caspases and execute cell death, and finally w

72 The activated form of caspase-8, an initiator caspase, and amyloid-beta, a product of APP pr

73 Caspase-9 is the initiator caspase, and its loss blocks effector caspase

74 reted to be proximity-driven dimerization of initiator caspases, and consequently their activation.

75 Initiator caspases are activated in response to death st

76 The molecular mechanisms by which initiator caspases are activated remain poorly understoo

77 According to dogma, initiator caspases are activated through proximity-induc

78 cytometry, and immunoblotting, we find that initiator caspases are active during the long and variab

79 It is widely believed that 'initiator' caspases are recruited to and activated withi

80 Caspase 8, the initiator caspase associated with Fas-mediated apoptosis

81 activated by the cleavage of caspase-8, the initiator caspase associated with the death receptor pat

82 Caspase-2 is considered an initiator caspase because its long prodomain contains a

83 and execution phases of cell death, with the initiator caspases being recruited to multicomponent sig

84 the decline in SK1 occurs downstream of the initiator caspase but upstream of the effector caspase.

85 mechanistic insights into the activation of initiator caspase by the apoptosome.

86 o that any free p20 formed by deacylation of initiator caspases cannot reassociate to active heterote

87 tor caspases (caspase 3, 6, 7, or 12) or the initiator caspases (caspase 8 or 9) could be detected in

88 de biochemical evidence that other apoptosis initiator caspases (caspase-2 and -10) as well as a proc

89 related activities of caspases, including an initiator caspase, caspase 8, and effector caspases, suc

90 he activity of the death receptor-associated initiator caspase, caspase 8, and is inhibited by the pe

91 Activity of the DR-associated initiator caspase, caspase 8, was also increased in the

92 ing to the recruitment and activation of the initiator caspase, caspase-9.

93 Therefore, like other initiator caspases, Caspase-1 activation by inflammasome

94 ved to have a low potency for inhibiting the initiator caspases, caspase-1 and caspase-8, and caspase

95 DP-ribose) polymerase, and activation of the initiator caspases, caspase-8 and -9, earlier in infecti

96 stern blots, indicated that each of the main initiator caspases, caspase-8, caspase-9, and caspase-12

97 ccompanied by activation of a combination of initiator caspases (caspases-2, -8, and -9) and executed

98 DR-proximal initiator caspases cleaved the clathrin adaptor subunit

99 provide evidence that ceramide generation is initiator caspase-dependent and occurs prior to commitme

100 Ceramide generation induced by FADD was initiator caspase-dependent, being blocked by crmA.

101 es DIAP2-dependent polyubiquitylation of the initiator caspase DREDD.

102 CRINKLED (CK) selectively interacts with the initiator caspase DRONC and regulates some of its non-ap

103 It was found that the initiator caspase Dronc and the proapoptotic gene head i

104 This set includes the Drosophila initiator caspase Dronc and, surprisingly, several metab

105 The initiator caspase Dronc does not cleave dSREBP, but anim

106 The initiator caspase Dronc is the only CARD-domain containi

107 The initiator caspase Dronc is the only Drosophila caspase t

108 phila effector caspase DrICE or its upstream initiator caspase DRONC prevented FHV-induced apoptosis

109 wing that cellular NADPH levels modulate the initiator caspase Dronc through its phosphorylation at S

110 In living glands, activation of the initiator caspase dronc triggers cortical F-actin disman

111 This proliferation relies on the initiator caspase Dronc, and occurs independent of JNK,

112 This process is under control of the initiator caspase Dronc, but not effector caspases.

113 nfirming that P49 targeted DrICE and not the initiator caspase DRONC, depletion of DrICE by RNA silen

114 bitor p35 or a dominant-negative form of the initiator caspase Dronc, is sufficient to inhibit saliva

115 in Drosophila IAP1 (DIAP1) and displaces the initiator caspase DRONC.

116 of DNA-damage sensing but that requires the initiator caspase Dronc.

117 x, which culminates in the activation of the initiator caspase, either caspase-8 or caspase-10.

118 as distal to activation of several different initiator caspases, evidence for a further downstream pr

119 The autocatalytic activation of an initiator caspase, exemplified by caspase-9 in mammals o

120 ctivation is started by trans-cleavage by an initiator caspase followed by autocleavage of effector c

121 Caspase 8, the initiator caspase for death receptor-induced apoptosis,

122 Here, we show that the initiator caspase for the intrinsic pathway is activated

123 e a downstream caspase that depends on other initiator caspases for activation.

124 Specific adaptors regulate the activation of initiator caspases; for example, FADD and Apaf-1 engage

125 ap1 silencing was rescued by cosilencing the initiator caspase gene Aedronc, indicating that the mort

126 In summary, we demonstrate that an apoptotic initiator caspase has a very critical non-apoptotic func

127 inally proposed to explain the activation of initiator caspases, has recently been reinterpreted to b

128 lly, b-VAD-fmk failed to label any activated initiator caspase in Apaf-1-deficient cells exposed to h

129 rthermore, we determined that caspase-10, an initiator caspase in death receptor signaling, is the mo

130 In response to TRAIL, caspase-8, an initiator caspase in death receptor-mediated apoptosis,

131 utocatalytic cleavage of Dronc, an important initiator caspase in Drosophila, results in a drastic en

132 ase inhibitor, we identified caspase-2 as an initiator caspase in EtOH-treated corneal fibroblasts.

133 previously unknown role for caspase-2 as an initiator caspase in lipoapoptosis and suggest that casp

134 We investigated whether the initiator caspase in the extrinsic pathway, caspase-8, o

135 and reduced activations of both effector and initiator caspases in high cell density, postconfluent C

136 n neuronal injury, direct evidence of active initiator caspases in stroke and the functional relevanc

137 evidence for the differential involvement of initiator caspases in the apoptotic volume decrease duri

138 Activation of initiator caspases in the dying cells stimulates the pro

139 To define the potential role of initiator caspases in vivo, we tested the effect of cell

140 ignificantly reduced the expression level of initiator caspases, increased the ratio of XIAP to Smac/

141 ion motif is required but not sufficient for initiator caspase inhibition by P49.

142 Caspase-8, an initiator caspase involved in lymphocyte apoptosis, is p

143 Activation of an initiator caspase is essential to the execution of apopt

144 This role in regulating initiator caspases is an entirely novel role for the PAK

145 Enzymatic activation of initiator caspases leads to proteolytic activation of do

146 Thus we demonstrate a direct role for initiator caspase-mediated proteolysis in promoting gene

147 induced equilibrium folding/unfolding of two initiator caspases, monomeric caspase-8 and cFLIP(L), ov

148 In Drosophila, the initiator caspase Nc (previously Dronc) cleaves and acti

149 etected in dying cells, and neither of these initiator caspases nor the endoplasmic reticulum stress-

150 so detected the activation of caspase 8, the initiator caspase of the death receptor pathway.

151 Caspase-8 is the initiator caspase of the extrinsic apoptosis pathway and

152 e examine the role of caspase-9 (Casp9), the initiator caspase of the intrinsic apoptotic cascade, in

153 quitous IAP, can inhibit both caspase-9, the initiator caspase of the mitochondrial apoptotic pathway

154 The binding pocket has been observed in initiator caspases of other species.

155 heat shock does not require any of the known initiator caspases or their activating complexes to prom

156 ability of the caspases as the substrates of initiator caspases or upstream regulators without impact

157 CA) into two distinct subfamilies: monomers (initiator caspases) or dimers (effector caspases).

158 Binding of initiator caspase precursors to activator molecules appe

159 lammatory signaling is the activation of the initiator caspase, procaspase-1.

160 NOS) inhibitors regulate S-nitrosation of an initiator caspase, procaspase-9, in a human colon adenoc

161 eptors include the adaptor protein FADD, the initiator caspases procaspases-8 and -10 and the regulat

162 These data indicate that activated initiator caspases provide another effective target for

163 Procaspase-8, an initiator caspase recruited to death receptors, is activ

164 esults suggest that caspase-11 is a critical initiator caspase responsible for the activation of casp

165 Examination of initiator caspases revealed the cleavage of caspase 9 bu

166 uring apoptosis occurs in a cascade from the initiator caspase(s) (e.g. caspase-8) to the effector ca

167 other basal deuterostomes, signal-responsive initiator caspase subfamilies (caspases-8/10 and 9, whic

168 as caspase-7 via a linker_BIR2 fragment and initiator caspases such as caspase-9 via the BIR3 domain

169 Caspase-8 is an initiator caspase that becomes activated when Fas death

170 otif, P49 was impaired for inhibition of the initiator caspase that cleaves and activates pro-Sf-casp

171 el in vivo specificity for a P35-insensitive initiator caspase that functions at an evolutionarily co

172 apoptosis in invertebrates by inhibiting an initiator caspase that is P35 insensitive.

173 e consistent with the involvement of a novel initiator caspase that is resistant to P35, but directly

174 sulted in isolation of caspase-8 (Casp8), an initiator caspase that mediates death-receptor-induced a

175 These results indicate that levels of initiator caspases vary widely among different leukemia

176 No evidence of TcapQ647 cleavage by initiator caspases was observed.

177 To define the roles of specific initiator caspases, we utilized Jurkat cells genetically

178 e to TRAIL or TNF requires the activation of initiator caspases, which then activate the effector cas

179 nc mutation, indicating that dronc is a main initiator caspase, while strica plays a minor role that

180 roduced after adapter-mediated clustering of initiator caspase zymogens.