ライフサイエンスコーパス: inside-out

1 HCl(3)/MeOH, viscumin A is in effect turned "inside out".

2 aits that, in concert, can protect the plant inside out.

3 examine the incidence and time dependence of inside-out abrasion in asymptomatic patients implanted w

4 y has posed a unique clinical scenario where inside-out abrasion results in externalization of conduc

5 ectric failure and mechanical separation via inside-out abrasion.

6 motaxis in vitro and in vivo with failure of inside-out activation and trafficking of the Mac1 integr

7 kindlin modifies the molecular mechanisms of inside-out activation by decreasing the crossing angle b

8 ier in integrin activation, and suggest that inside-out activation in intact cells may involve confor

9 ent conformation with disulfide bonds resist inside-out activation induced by cytoplasmic domain muta

10 ation, secretion, and integrin alphaIIbbeta3 inside-out activation induced by several agonists were n

11 In the absence of inside-out activation ligand, binding to LFA-1 is extrem

12 n the basis of the results, we propose a new inside-out activation mechanism for integrin alpha(IIb)b

13 ndependent pathological activation is a weak inside-out activation mediated by intracellular kinase d

14 genetic variability in proteins that mediate inside-out activation of alphaIIbbeta3 The RASGRP2 gene

15 ting monocytes through beta(2) integrins and inside-out activation of beta(2) integrins by monocyte c

16 Altogether, these studies establish that inside-out activation of beta1 integrins promotes tumor

17 s is critically dependent on agonist-induced inside-out activation of heterodimeric integrin receptor

18 elet aggregation was accompanied by impaired inside-out activation of integrin alpha(IIb)beta(3) and

19 nd PI3Kbeta in integrin alpha2beta1 promoted inside-out activation of integrin alphaIIbbeta3 and thro

20 Second messenger-mediated inside-out activation of integrin alphaIIbbeta3 is a key

21 A axis suppresses phagocytosis by inhibiting inside-out activation of integrin signaling in the macro

22 nderlie the cell adhesion phenotypes through inside-out activation of integrin signaling.

23 Although talin binding is sufficient for inside-out activation of integrin, other cytoplasmic pro

24 actin-binding protein that controls both the inside-out activation of integrins and actin filament an

25 tems, we show that Akt1 is essential for the inside-out activation of integrins in endothelial cells

26 Inside-out activation of integrins is mediated via the b

27 signals involving TLR2 and PI3k that promote inside-out activation of Mac-1, thereby enhancing spore

28 al changes in the TM domains associated with inside-out activation.

29 ein, and the lipid bilayer promotes integrin inside-out activation.

30 s occurred in the TMs upon alpha(IIb)beta(3) inside-out activation.

31 re or after soluble ligand binding or during inside-out activation.

32 )-mediated activation of nmMYLK resulted in "inside-out" activation of beta1 integrin, followed by "o

33 -methionyl-leucine-phenylalanine to trigger "inside-out" activation, the effects of hyperoxia are rev

34 ssociated Protein-1)-mediated modulation of "inside-out" activation.

35 ich mediates Rap1 activation during platelet inside-out alphaIIbbeta3 signaling.

36 , with embolism formation occurring from the inside out and refilling from the outside in.

37 oocytes and measured unitary currents in the inside-out and cell-attached modes of the patch-clamp te

38 Membrane proteins play vital roles in inside-out and outside-in signal transduction by respond

39 n receptor plays key roles in mediating both inside-out and outside-in signaling between cells and th

40 fetimes of cell surface attachments for both inside-out and outside-in signaling exhibit single-bond-

41 In this article, we examine the impact of inside-out and outside-in signaling in neutrophils on th

42 ration of these domains is required for both inside-out and outside-in signaling, the role of TM homo

43 e plasma membrane in pathways referred to as inside-out and outside-in signaling.

44 ells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions

45 odels to take a comprehensive outside-in and inside-out approach at exploring how integrin alphaIIbbe

46 fiber implantation, our method provides an "inside-out" approach to deliver nanoscopic light emitter

47 istry and biology have come together in the "inside-out" approach to enzyme engineering.

48 , Terada and colleagues demonstrate a novel 'inside-out' attachment sensing role for the adapter prot

49 Within minutes, CR3 undergoes inside-out auto-activation that drives the downregulatio

50 However, a role for inside-out beta1 activation in tumor cell metastasis is

51 hough abscission could be organized from the inside out by the microtubule-based midbody or from the

52 rtery wall is constructed radially, from the inside out, by two separate but coordinated processes.

53 macromolecular complex functioned to amplify inside-out Ca(2+) signaling in response to IL-8 stimulat

54 The mechanistic underpinnings of this inside-out Ca(2+) signalling were largely undefined.

55 c cancer selectively affect the mechanism of inside-out cell surface regulation without inhibiting ba

56 pressed in Xenopus laevis oocytes, using the inside-out configuration of the patch clamp technique.

57 212-2-gated current (I(WIN)) was recorded in inside-out configuration.

58 Instead, CIU generates inside-out conformations where previously surface-expose

59 Thus, NHE9 mediates inside-out control of oncogenic signalling and is a high

60 a ZBP1-initiated nucleus-to-plasma membrane "inside-out" death pathway with potentially pathogenic co

61 On its own, computational "inside-out" design can lead to the production of catalyt

62 ed spiroligozymes) were designed, using the "inside-out" design strategy, and mutated synthetically t

63 Thus, initial segments assemble from the inside out driven by the intrinsic accumulation of ankyr

64 nverts changes in external K(+) into rapid, 'inside-out' electrical signaling to direct blood flow to

65 the concept of obligation develops "from the inside-out": emerging first in experiences of shared age

66 d recombinant pleckstrin homology domains to inside-out excised patches.

67 In inside-out-excised neuronal patch recordings, we found a

68 ted vagus and patch clamp and single-channel inside-out experiments showed that the effect of theophy

69 phase NPC assembly proceeds by an asymmetric inside-out extrusion of the INM.

70 ing this region, calmodulin regulates in an "inside-out" fashion the ectodomain shedding of the recep

71 esions and a chronically elevated outside-in/inside-out focal adhesion (FA) kinase (FAK)-Rho signalin

72 We find that contractility-based, inside-out forces are evenly distributed at the edges of

73 sized to occur due to modulation of cellular inside-out forces in response to changes in the external

74 dure that enabled us to image reconstructed, inside-out FtsZ rings by negative-stain EM, revealing th

75 The inside-out FtsZ rings moved back and forth along the tub

76 re that we discovered here for reconstituted inside-out FtsZ rings provides what to our knowledge is

77 iphosphine ((CH2)14)3 P (1) can rapidly turn inside-out (homeomorphic isomerization) to give a mixtur

78 hesis that teeth evolved before jaws and the inside-out hypothesis of dental evolution must be reject

79 on in the vertebrate lineage, inspiring the 'inside-out' hypothesis that teeth evolved independently

80 orces using nanonet force microscopy in both inside-out (I-O intrinsic contractility) and outside-in

81 colony-stimulating factor (M-CSF)-stimulated inside-out integrin activation and cytoskeleton organiza

82 The small GTPase Rap1 regulates inside-out integrin activation and thereby influences ce

83 Radil and negatively regulates Rap1-mediated inside-out integrin activation by tethering Radil on mic

84 , including advances in our understanding of inside-out integrin activation.

85 and spontaneously adhesive in the absence of inside-out integrin signaling but that LFA-1-mediated fi

86 roton fluxes indicated that IAR was inserted inside-out into our sealed LUV system, which we confirme

87 erichia coli, using right-side-out (RSO) and inside-out (ISO) membrane vesicles.

88 etach and go' model explains many aspects of inside-out lamination, defects in the Reeler mutant and

89 ing cascade to establish the characteristic "inside out" lamination pattern.

90 r excitatory and inhibitory neurons, and an "inside-out" layer formation of excitatory neurons as see

91 We outline a unified model for inside-out layering of the neocortex, hinging on a new i

92 The biological activity was determined in inside-out macropatches containing either homotetrameric

93 ates heterologously expressed rat NBCe1-A in inside-out macropatches excised from Xenopus laevis oocy

94 Depletion of endogenous PIP(2) in inside-out macropatches from Xenopus oocytes inhibited h

95 In excised inside-out macropatches of HEK293 cells, activation of w

96 Exposing the cytosolic side of inside-out macropatches to a 5% CO(2)/33 mM HCO(3)(-) so

97 urons do not integrate into the cortex in an inside-out manner but preferentially (75%) occupy superf

98 progenitors (RGPs) in a birth-date-dependent inside-out manner.

99 dies to special research cell designs or to 'inside-out' measurement approaches.

100 uired to modulate the FHOD1 activity and the inside-out mechanical coupling that tunes the cell inter

101 es compelling support for the importance of "inside out" mechanisms of AD.

102 Here we detail three passive, 'inside-out' mechanotransduction mechanisms with an empha

103 of these mutations by radiotracer efflux and inside-out membrane patch clamping in COSm6 cells expres

104 When applied to inside-out membrane patches expressing rat TRPA1, URB597

105 mon CF mutation, F508del-CFTR, using excised inside-out membrane patches from transiently transfected

106 P/Q-type channels was reconstituted in giant inside-out membrane patches from Xenopus oocytes.

107 in blocking TRPC5 single channels in excised inside-out membrane patches, hinting to a direct block o

108 ensitization can be recapitulated in excised inside-out membrane patches, reversed by strong reducing

109 d acutely suppresses P/Q channel activity in inside-out membrane patches, that this action requires C

110 in physiological saline, and yet in excised inside-out membrane patches, the Na+ EC50 for KNa channe

111 )P(2) levels and/or by adding PI(3,5)P(2) to inside-out membrane patches.

112 effect on BK(Ca) channel open probability in inside-out membrane patches.

113 pressed in Lactococcus lactis and studied in inside-out membrane vesicles and in purified form.

114 When inside-out membrane vesicles from each of these substitu

115 e cross-linking studies on the McjD dimer in inside-out membrane vesicles of E. coli confirmed the pr

116 cium transport measurements in intact cells, inside-out membrane vesicles, and proteoliposomes contai

117 take of tritiated estradiol glucuronide into inside-out membrane vesicles, their affinity for and abi

118 se-coupled H(+) transport was measured using inside-out membrane vesicles.

119 to "outside-in" primary immune mediated and "inside-out" metabolic stress of oligodendrocyte (OL) rel

120 It also suggests an inside-out model of signal transduction where VSV interr

121 These findings support an inside-out model of T cell triggering driven by small-mo

122 alaxies, star formation is quenched from the inside out, on time scales less than 1 billion years in

123 From the inside out or from the outside in?

124 notype-dependent effect was observed for the inside-out or outside-in physical skin barrier function.

125 cell-attached patches but failed to do so in inside-out or outside-out patches.

126 r at the plasma membrane requiring integrin 'inside-out' or 'outside-in' signalling.

127 protein was functionally reconstituted in an inside-out orientation.

128 The spheres develop via an inside-out Ostwald ripening mechanism.

129 s in all four configurations (cell-attached, inside-out, outside-out, and whole-cell).

130 ermined by RT-PCR, immunohistochemistry, and inside-out patch clamp in human trabecular meshwork (TM)

131 Using two-electrode voltage clamp and inside-out patch clamp recordings from Xenopus laevis oo

132 annexin V-based CaPLSase-imaging assay with inside-out patch clamp technique, we thoroughly characte

133 ombined with single-channel recording in the inside-out patch configuration showed that ATP efflux co

134 Inside-out patch experiments revealed that PD-307243 inc

135 d to the internal face of the membrane using inside-out patch recording.

136 tigated this point using both whole-cell and inside-out patch recordings from human Na(v)1.7 channels

137 nels were detected in both cell-attached and inside-out patch recordings in C6 cells expressing Cx43,

138 en applied directly as a purified peptide in inside-out patch recordings.

139 ssion, as assessed by whole-cell and excised inside-out patch recordings.

140 ving this process by using two-electrode and inside-out patch voltage clamp in normal and truncated (

141 In excised inside-out patch-clamp measurements, ATP reactivated the

142 d in human embryonic kidney 293 cells, using inside-out patch-clamp recordings.

143 ressed in HEK-293 cells using whole-cell and inside-out patch-clamp recordings.

144 hatase 1, inhibits NDPK-B-activated TRPV5 in inside/out patch experiments.

145 ANO1 currents in excised inside-out patches activated by 270 nM Ca(2+) at +100 mV

146 +) (10 mum) applied to the cytosolic side of inside-out patches activated the 20 pS channel.

147 Zn(2+) to the intracellular face of excised, inside-out patches activates TRPA1 with an EC(50) value

148 K(ATP) channel activity was measured in inside-out patches and plasma membrane potential in perf

149 Addition of FMRP to inside-out patches containing native Aplysia Slack chann

150 In inside-out patches diC8-PIP(2) also inhibited TRPC6 acti

151 d CNBD, application of cyclic nucleotides to inside-out patches did not affect currents recorded from

152 a water soluble form of PIP(2), to quiescent inside-out patches evoked single channel currents with a

153 lar magnitude in cell-attached patches as in inside-out patches exposed to 10 mm MgATP.

154 Single channel recordings of excised inside-out patches from the apical membrane of aldostero

155 Utilizing inside-out patches from Xenopus oocytes heterologously e

156 ified Gbetagamma proteins applied to excised inside-out patches inhibited TRPM3 currents, indicating

157 ent chemicals; no activation was observed in inside-out patches unless a polyphosphate was present.

158 matched the maximum activation achieved with inside-out patches with zero cytosolic Ca(2+), whereas t

159 In inside-out patches, currents were inhibited strongly by

160 In excised inside-out patches, GsMTx4 sensitized both channels to t

161 In whole-cell recordings and inside-out patches, H(2)O(2) or diamide caused a strong

162 In excised, inside-out patches, the same method of FN application le

163 K(ATP) channel density, recorded in excised inside-out patches, was larger at the cell end when comp

164 id and allowed channel activation by heat in inside-out patches.

165 ol 4,5-bisphosphate (PI(4,5)P(2)) in excised inside-out patches.

166 experiments and when transiently applied to inside-out patches.

167 but had little effect on channel opening on inside-out patches.

168 , H(2)O(2) did not directly activate ENaC in inside-out patches.

169 by acidification of the cytosolic surface of inside-out patches.

170 in the cell-attached patches but not in the inside-out patches.

171 0 nm Ang II-evoked TRPC6 channel activity in inside-out patches.

172 er the ability of PIP(2) to activate SOCs in inside-out patches.

173 l or macroscopic currents were recorded from inside-out patches.

174 ctivate TRPA1, also failed to activate it in inside-out patches.

175 Hypoxia did not inhibit BK in inside-out patches.

176 ly rising ICl was recorded in whole-cell and inside-out patches.

177 y when applied to the internal surface using inside-out patches.

178 le-cell dialysis, as well as when applied to inside-out patches.

179 els inactivate upon prolonged stimulation in inside-out patches; this "rundown" is due to PIP(2) depl

180 Inhibition of kindlin-dependent steps in the inside-out pathway as an approach to block platelet aggr

181 The importance of integrin inside-out pathway in vascular physiology has been unequ

182 nction, the identity of the T cell receptor "inside-out" pathway for lymphocyte function-associated a

183 Here, we define the "inside-out" pathway mediated by N-terminal SKAP1 (SKAP-5

184 Overall, our findings define a TCR "inside-out" pathway via N-SKAP1-C-RapL that regulates T

185 rgic projections within the cortex follow an inside-out pattern of innervation.

186 pregnated neurons conformed to the expected "inside-out" pattern of development, meaning that cells p

187 However, the mechanism is probably "inside-out": pharmacological chaperoning in the endoplas

188 olves a novel process whereby large, intact, inside-out phosphatidylserine (PS)-exposed autophagic ve

189 Herein we report an inside-out preinstallation-infusion-hydration method for

190 The "inside out" proponents derive support from evidence that

191 circulating mature reticulocytes expressing inside-out PS-exposed autophagic vesicles because of asp

192 is of radial-glia neural progenitors (RGCs), inside-out radial migration of post-mitotic glutamatergi

193 These results suggest that RGP-guided inside-out radial neuronal migration facilitates the ini

194 pletion of DISABLED-1, which compromises the inside-out radial neuronal migration pattern in the deve

195 ellular catalytic domain can be activated by inside-out regulation is not completely understood.

196 d a fluorescent probe that shed light on the inside-out regulation of one of the major leukocyte inte

197 We propose that inside-out regulation of protein exchange modulates adhe

198 lipodia formation, suggesting a key role for inside-out S1P signaling.

199 Reelin is essential for the stereotypical inside-out sequential lamination of the neocortex, but t

200 eric membrane proteins that uniquely mediate inside-out signal transduction, whereby adhesion to the

201 tant gap in understanding substrate-specific inside-out signal transfer along cleaved transmembrane p

202 This inside-out signal transfer required substrate homodimeri

203 These cells exhibit increased beta2 integrin inside-out signaling (binding affinity and avidity), and

204 dlins are cytoplasmic molecules that mediate inside-out signaling and activation of the integrins.

205 ontractility mediates integrin signaling via inside-out signaling and emphasizes the importance of ma

206 ional states independently regulated by both inside-out signaling and ligand binding.

207 critical role in integrin alpha(IIb)beta(3) inside-out signaling and platelet aggregation.

208 dulates ethanol inhibition of L1 adhesion by inside-out signaling and that differential regulation of

209 required for ligand binding during integrin inside-out signaling and that the deadbolt does not regu

210 The molecular mechanisms underlying inside-out signaling are not completely understood.

211 gain of function is not because of enhanced inside-out signaling because granular secretion, Thrombo

212 s, licensing controls signals as proximal as inside-out signaling by activating receptors but not int

213 zation step is mediated by the activation of inside-out signaling by integrins and by the secretion o

214 In toto, our data suggest that inside-out signaling by specific residues in the cytopla

215 a1, beta2 and beta3 integrins, but defective inside-out signaling causes immune deficiency and bleedi

216 telets, suppressing classic thrombin-induced inside-out signaling events (eg, Akt activation, intrace

217 ssue macrophages and the requirement of TLR2 inside-out signaling for CR3 exploitation by P. gingival

218 ngs provide evidence for a novel paradigm of inside-out signaling in platelets, whereby beta3 integri

219 This indicates the existence of inside-out signaling in the EGF receptor system.

220 Remarkably, inside-out signaling induced by each one of these recept

221 Inside-out signaling is mediated by binding adaptor prot

222 lets, is known to regulate receptor-mediated inside-out signaling leading to integrin activation and

223 regulates integrin activation, TCR-initiated inside-out signaling may induce a conformational change

224 E-cadherin present at the cell surface by an inside-out signaling mechanism is important in cancer.

225 at upon binding Syk opens the receptor by an inside-out signaling mechanism that amplifies BCR signal

226 rolling activation of these receptors via an inside-out signaling mechanism, but the precise structur

227 egrin signaling in HTM cells, possibly by an inside-out signaling mechanism.

228 most active, which has implications for the inside-out signaling mechanism.

229 We present evidence that LapD utilizes an inside-out signaling mechanism: binding c-di-GMP in the

230 , with activators, inhibitors, and elaborate inside-out signaling mechanisms controlling its conforma

231 Moreover, the inside-out signaling of RAP1 activation is coordinated w

232 mechanism impairs the activation of a major inside-out signaling pathway that triggers the conformat

233 pon denervation, demonstrating an unexpected inside-out signaling pathway; the receptor up-regulation

234 Upon platelet activation, inside-out signaling pathways increase the affinity of a

235 1), can directly participate in the platelet inside-out signaling process.

236 Inside-out signaling regulation of the beta2-integrin le

237 e as compared with controls, suggesting that inside-out signaling remains intact.

238 duce a cascade of signaling events termed as inside-out signaling that culminate in exposure of high-

239 apid activation of Pyk2 and JNK, followed by inside-out signaling that leads to cell detachment-induc

240 through Itgbeta1 to drive cell invasion, and inside-out signaling that maintains tumor cell-matrix co

241 TLR2 proadhesive pathway is characterized by inside-out signaling that transactivates beta(2) integri

242 V-containing ligand to study the role of the inside-out signaling through formyl peptide receptor and

243 modulates adhesion of T cells by regulating inside-out signaling through LFA-1.

244 Inside-out signaling to integrins is mediated by the sma

245 Inside-out signaling to integrins is mediated by the sma

246 l cytotoxicity and that steps as distinct as inside-out signaling to LFA-1 and signals for granule re

247 -1 properties but was instead due to reduced inside-out signaling to LFA-1 by activating receptors.

248 extracellular transportation of S1P and its inside-out signaling via S1P1.

249 The small G protein Rap1 can mediate "inside-out signaling" by recruiting effectors to the pla

250 phils in Mg2+ plus the chemokine IL-8 (i.e., inside-out signaling) induces several-hundred-fold longe

251 leads to alphaIIbbeta3 integrin activation (inside-out signaling) is not clearly defined.

252 cated in integrin activation in neutrophils (inside-out signaling).

253 in the GPIb-IX-mediated integrin activation (inside-out signaling).

254 lin-2-mediated integrin activation (integrin inside-out signaling).

255 This occurs through a process known as inside-out signaling, which has been shown to require se

256 or P-selectin glycoprotein ligand-1-induced inside-out signaling.

257 and to perform proper alphaIIbbeta3 integrin inside-out signaling.

258 R7 activate LFA-1 through processes known as inside-out signaling.

259 ily members as crucial mediators of integrin inside-out signaling.

260 ta(3) outside-in signaling in the absence of inside-out signaling.

261 found in the active state in the absence of inside-out signaling.

262 pha4 tail and activated alpha4 integrins via inside-out signaling.

263 namically regulated through a process termed inside-out signaling.

264 lular signals from CD36, similar to integrin inside-out signaling.

265 /AKT activation, thereby initiating integrin inside-out signaling.

266 ITAM to recruit activated Syk family kinases Inside-out signaling: signals initiated by engagement of

267 is a multifunctional adapter that regulates "inside-out" signaling from the TCR to integrins.

268 of responsiveness of LFA-1 to SDF-1-induced "inside-out" signaling involving CXCR4 and Lyn, leading t

269 ble IFNAR1 degradation, the existence of an "inside-out" signaling that accelerates IFNAR1 turnover i

270 ding of controls involving "outside-in" and "inside-out" signaling that restrain cilium assembly.

271 in into TCR-induced adhesive junctions, and "inside-out" signaling to beta1 integrins.

272 ly to their ligands but become active after 'inside-out' signaling through other membrane receptors.

273 Inside-out signalling activates integrins through a tali

274 The first talin-binding wave mediates inside-out signalling and also ligand-induced integrin a

275 t affect platelet activation markers such as inside-out signalling to integrin alpha(IIb)beta(3) or P

276 Each receptor was capable of inducing inside-out signals for LFA-1, promoting adhesion, but no

277 nd avidity of integrins can be regulated via inside-out signals from other receptors.

278 Strong inside-out signals induced by the combination of NKG2D a

279 d platelet aggregation, providing proof that inside-out signals that activate alpha(IIb)beta(3) requi

280 quired for activation of the GTPase Rap1 and inside-out signals that promote integrin adhesion.

281 CD14, independently of TLR/MyD88-induced or inside-out signals.

282 tion of beta1 integrins can be regulated by "inside-out" signals leading to extravasation from the ci

283 dence that epidermal Par3 loss disturbed the inside-out skin barrier, coinciding with altered express

284 se results delineate a novel force-activated inside-out Src/PI3K/FAK/Akt pathway by which cancer cell

285 However, activation of Rap1b and inside-out stimulation of integrin alphaIIbbeta3 were re

286 Here, the authors show an inside-out strategy to synthesize multilayered polymer c

287 Here, we demonstrate an inside-out technique for creating multilayered polymer c

288 that explain the ability of talin to mediate inside-out TM signalling.

289 heterocomplex and the mechanism of integrin inside-out transmembrane signaling.

290 re, we examined the molecular basis of this "inside-out" triggering mechanism.

291 Our findings provide insight into the "inside-out" triggering of Vgamma9Vdelta2 T cell activati

292 We tested each of them directly in inside-out TRP-expressing patches excised from the rhabd

293 w the key signaling events that occur in the inside-out versus outside-in pathways, highlighting rece

294 brane translocation in a fully reconstituted inside-out vesicle system.

295 screening (VLS) were tested in vitro, using inside-out vesicles (IOV), for inhibition of cGMP efflux

296 es from our laboratory using plasma membrane inside-out vesicles (ISOV) prepared from yeast expressin

297 gy with the sequence seen during spontaneous inside-out vesiculation of erythrocyte membranes suggest

298 sh that the channels are inserted uniformly "inside-out" with their cytoplasmic surface facing the me

299 The inside-out Z rings were highly dynamic, and generated a

300 This assembled 'inside-out' Z rings that wrapped around the outside surf