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1 protein complexes of IN with viral DNA ends (intasomes).
2 rge nucleoprotein complex, which we call the intasome.
3 factor is required for reconstitution of the intasome.
4 he native structure of the HIV-1 protein-DNA intasome.
5 tegration mediated by the PIC but not by the intasome.
6 viral DNA (vDNA) ends in a complex termed an intasome.
7 r virus (MMTV) strand transfer complex (STC) intasome.
8 nd to the active site of the deltaretroviral intasome.
9 2A-H2B subunits to allow engagement with the intasome.
10 with two viral DNA ends forms the functional intasome.
11 in within a nucleoprotein assembly called an intasome.
12 NA long terminal repeat (LTR) ends termed an intasome.
13 ructure of the HIV-1 strand transfer complex intasome.
14 l DNA ends in a stable complex, known as the intasome.
15 ng dimer in the crystal structure of the PFV intasome.
16 orts to preferentially stabilize an excisive intasome.
17 ome but inhibits formation of an integrative intasome.
18 DNA bridged by integrase within the excisive intasome.
19  required for assembly of recombinogenic Cre intasomes.
20 gher order recombination structures known as intasomes.
21 ith earlier studies on prototype foamy virus intasomes.
22 perly arranged protein-DNA structures termed intasomes.
23 d by the PIC for integration compared to the intasomes.
24 ation of integrase (IN)-DNA complexes termed intasomes.
25 een critical for structural studies of HIV-1 intasomes.
26 e also seen with both wild-type and Sso7d-IN intasomes.
27  regulated by the environment encountered by intasomes.
28 teractions critical for assembly of both RSV intasomes.
29 ravel the functional mechanisms in different intasomes.
30  simultaneously with IN and viral DNA within intasomes.
31 y the integration host factor IHF, forms the intasome, a higher order structure, within which Int, a
32                                          The intasome, a higher-order nucleoprotein complex composed
33 f retroviral replication, is mediated by the intasome, a multimer of integrase (IN) assembled on vira
34 uired for the production of a recombinagenic intasome, a mutant attP DNA deficient in binding of the
35              This process is operated by the intasome, a nucleoprotein complex composed of an integra
36       The cleft between IN dimers within the intasome accommodates chromosomal DNA in a severely bent
37 clarified the INSTI binding modes within the intasome active sites and helped elucidate an important
38 retroviruses that assemble unique multimeric intasomes, although the number of protomers in the intas
39             Insight into the deltaretroviral intasome and its interaction with the host will be cruci
40 lucidate the binding of INSTIs to the HTLV-1 intasome and support their use for pre-exposure prophyla
41 ide an accurate depiction of interactions of intasomes and INSTIs to be leveraged for structure-based
42 re observed in cryo-EM studies of lentiviral intasomes and suggest that this organization underlies m
43 t of the high-resolution structures of HIV-1 intasomes are based on an HIV-1 IN with an Sso7d protein
44                                              Intasomes are capable of integrating the DNA termini in
45 l/target DNA substrates have indicated these intasomes are composed of IN subunits ranging from tetra
46 ggesting the disordered outer domains of PFV intasomes are not required for intasome assembly or inte
47          High-resolution structures of HIV-1 intasomes are required to understand the mechanism of IN
48                                              Intasomes are targeted by the latest generation of antir
49      Herein we present a model for the HIV-1 intasome assembled using the PFV structure as template.
50       The improved biophysical properties of intasomes assembled with LEDGF peptide fusion IN have en
51 X1 addition stimulates the activity of HIV-1 intasomes assembled with the unprocessed viral DNA but n
52 his by biochemical and structural studies of intasomes assembled with wild-type HIV-1 IN.
53 and S124 in cleaved synaptic complex and STC intasome assemblies and their catalytic activities, demo
54  work highlights the diversity of retrovirus intasome assembly and provides insights into the mechani
55                                      But how intasome assembly is controlled remains unclear.
56 omains of PFV intasomes are not required for intasome assembly or integration.
57  of nucleoprotein interactions important for intasome assembly.
58 s suggest that these may be intermediates in intasome assembly.
59  ingress, IN functions within an oligomeric "intasome" assembly to catalyze viral DNA integration int
60 -electron microscopy to visualize the HTLV-1 intasome at 3.7- angstrom resolution.
61 ualize the functional maedi-visna lentivirus intasome at 4.9 angstrom resolution.
62 d flexibility into the DNA, did not increase intasome binding or targeted integration.
63      Histone tails also significantly affect intasome binding, but have little impact on PFV integrat
64 ome to assay integration activity as well as intasome binding.
65 ith integration efficiency and suggests most intasome-binding events are unproductive.
66 ty of PICs-extracted from infected cells-and intasomes, biochemically assembled PIC substructures usi
67        Challenges associated with lentiviral intasome biochemistry have hindered high-resolution stru
68 w cryo-EM structures of drug-resistant HIV-1 intasomes bound to DTG or 4d, with better than 3- angstr
69 n cryo-electron microscopy structures of HIV intasomes bound to the latest generation of INSTIs.
70 ent, which promotes assembly of the excisive intasome but inhibits formation of an integrative intaso
71 d, TNPO3 effectively bound to the functional intasome but not to naked viral DNA, suggesting that TNP
72 ic acid (PCA), enhance the integration of FL intasomes by preventing aggregation.
73 edicts further ways in which the role of the intasome can be explored.
74 ion with photobleaching reveals that the MVV intasome can bind a variable number, up to sixteen molec
75 stituting nucleoprotein complexes possessing intasome characteristics.
76  the outer CTDs enhances the lifetime of the intasome compared to full length (FL) IN or deletion of
77 propose that integration mediated by the Ty3 intasome complex (IN and cDNA) is subject to inputs from
78 he formation of specific integrase-mIHF-attP intasome complexes.
79 gration of molecular mechanisms performed by intasomes (complexes containing viral DNA, IN, and its c
80 ecombination, demonstrates the importance of intasome components on the directionality of the reactio
81                                          The intasome, composed of a tetramer of integrase bridging a
82                                          The intasome comprises a homo-hexadecamer of IN with a tetra
83 ure shows the organization of the retroviral intasome comprising an integrase tetramer tightly associ
84  break/gap site without inducing substantial intasome conformational changes.
85                                          Ty3 intasome contacts a region of TBP, a subunit of TFIIIB,
86 residues are critical for the assembly of an intasome containing IN octamers but not for an intasome
87 tasome containing IN octamers but not for an intasome containing IN tetramers.
88 ers to assemble two viral DNA molecules into intasomes containing IN tetramers in contrast to one pos
89 th the unprocessed viral DNA but not that of intasomes containing the 3'-processed viral DNA.
90 ion is catalyzed by a nucleoprotein complex (Intasome) containing the viral integrase (IN) and the re
91 c, and hexadecameric, highlight how a common intasome core architecture can be assembled in different
92 ight how HIV-1 can use the common retroviral intasome core architecture to accommodate different IN d
93 te on viral DNA ends to assemble a conserved intasome core machine that facilitates integration.
94                                The conserved intasome core region consisting of IN subunits contribut
95 ing critical carboxy-terminal domains to the intasome core that cannot be provided in cis because of
96 relative to the IN subunits in the conserved intasome core, similar to results previously shown with
97  tetramer, and it is generally believed that intasomes derived from other retroviral genera use tetra
98 arious proteins and DNA binding sites in the intasome determine not only whether recombination can oc
99 ution structure of the prototype foamy virus intasome engaged with a nucleosome core particle.
100                                          The intasome engages chromosomal DNA within a target capture
101 or acquires a dependence on supercoiling for intasome formation and recombination.
102 ave begun to categorize the requirements for intasome formation in the site-specific recombination sy
103 rotein-DNA interfaces involved in lentiviral intasome formation.
104 e intramolecular Int-mediated bridges during intasome formation.
105 and Int-L5, readily recombines with a second intasome formed by Int-L5, mIHF and attL DNA (intasome-L
106  we have shown that the assembled complexes (intasomes) formed in vitro with linear 3.6-kbp DNA donor
107     Here we report crystal structures of the intasome from prototype foamy virus in complex with targ
108                        Reconstitution of the intasome from the maedi-visna virus (MVV), an ovine lent
109 essed ends were protected by IN in assembled intasomes from DNase I digestion up to approximately 20
110              Recent structures of lentiviral intasomes from simian immunodeficiency virus (SIV) and H
111 ization of integrase-viral DNA complexes, or intasomes, from the spumavirus prototype foamy virus rev
112 stal structures of the prototype foamy virus intasome have shown that all three FDA-approved drugs, r
113                                        HIV-1 intasomes have been refractory to high-resolution struct
114                                         Both intasomes have similar catalytic activities.
115 tructures of wildtype HIV-1 IN tetramers and intasome hexadecamers.
116 ed to form the native structure of the HIV-I intasome in infected cells.
117        Treatment with high salt disrupts the intasome in parallel with loss of intermolecular integra
118 tion is mediated by nucleoprotein complexes (intasomes) in which a pair of viral DNA ends are bridged
119  protein-DNA interactions that form the attP intasome (intasome-P) or the capture of attB, but acts l
120                                          PFV intasome integration to 601 nucleosomes occurs in cluste
121 sted that DNA target-site recognition limits intasome integration.
122                                      How the intasome interfaces with chromosomal DNA, which exists i
123 of U3 suggesting that a transient structural intasome intermediate was identified.
124                                      The HIV intasome is a large nucleoprotein assembly that mediates
125 n of specific protein-DNA architectures; the intasome is constructed by the formation of intramolecul
126 ggest that the integrase tetramer within the intasome is different from the integrase tetramer formed
127 we show that the prototype foamy virus (PFV) intasome is proficient at stable capture of nucleosomes
128                                          The intasome is the basic recombination unit of retroviral i
129 in contributes to the heterogeneity of HIV-1 intasomes is therefore raised.
130        Formation of such complexes, known as intasomes, is required for site-specific recombination c
131 ntasome formed by Int-L5, mIHF and attL DNA (intasome-L) to generate the attP and attB products of ex
132                       Interestingly, optimal intasome-mediated integration required the cellular cofa
133                 Structures of drug-bound PFV intasomes moreover elucidated the mechanism of inhibitor
134 (IN) synapses viral DNA ends within a stable intasome nucleoprotein complex for integration into a ho
135 troviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze insertion of
136 yo-electron microscopy reveals a multivalent intasome-nucleosome interface involving both gyres of nu
137            The octameric architecture of the intasome of mouse mammary tumour virus provides new insi
138 ted octameric integrase architecture for the intasome of the betaretrovirus mouse mammary tumour viru
139                                 However, the intasomes of orthoretroviruses, which include all known
140 ino and carboxyl terminal domains of the PFV intasome outer subunits during integration to a nucleoso
141 NA interactions that form the attP intasome (intasome-P) or the capture of attB, but acts later in th
142 ion of IN in higher order oligomerization of intasomes, potentially informing future strategies to pr
143  report cryo-EM structures of the lentiviral intasome prior to engagement of target DNA and following
144 ma virus (RSV) strand transfer complex (STC) intasome produced with IN and a preassembled viral/targe
145 h the RSV octameric cleaved synaptic complex intasome produced with IN and viral DNA only.
146                                   Retrovirus intasomes purified from virus-infected cells contain the
147 NA bend, and facilitates the formation of an intasome-R complex.
148 igher-order multimers, can form a functional intasome, reconcile the bulk of early HIV-1 IN biochemic
149                 Formation of attR-containing intasomes requires only Int and Xis, distinguishing Puko
150                                    Stacks of intasomes resulting from domain swapping are also seen w
151                          The majority of PFV intasome search events were non-productive.
152 t the prototype foamy virus (PFV) retroviral intasome searches for an integration site by one-dimensi
153 cryo-EM reveal a similar range of multimeric intasome species as with Sso7d-IN with the same common c
154        The propensity to assemble multimeric intasome species is, therefore, an intrinsic property of
155 smFRET), we show prototype foamy virus (PFV) intasomes specifically bind to DNA strand breaks and gap
156 data advance our understanding of retroviral intasome structure and function, as well as factors that
157 uirement for detailed understanding of HIV-1 intasome structure and function.
158          In parallel, the native protein-DNA intasome structure detected at the ends of HIV-1 by Mu-m
159 nucleoprotein complexes displayed the native intasome structure detected in wild-type preintegration
160 rences that could be important in quaternary intasome structure.
161 laces constraints on models for the excisive intasome structure.
162 s advance our understanding of different RSV intasome structures and molecular determinants involved
163 g and stabilizing a DNA bend that alters the intasome structures formed during recombination.
164                          The recently solved intasome structures of different retroviral species, whi
165  substrates and in forming higher-order attL intasome structures.
166  CTD of the outer monomers are disordered in intasome structures.
167                    The CTDs of the outer PFV intasome subunits dramatically affect nucleosome binding
168           The self-association properties of intasomes suggest that the integrase tetramer within the
169  in the buried interior of the modeled HIV-1 intasome, suggesting that even very subtle fitness effec
170 mes with structures of Prototype Foamy Virus intasomes suggests a binding mode for target DNA prior t
171  DNA breaks or gaps as modulators of dynamic intasome-target DNA interactions that encourage site-dir
172 ly truncated IN (residues 1-269) produced an intasome that contained tetramers but failed to produce
173 e construction of higher-order nucleoprotein intasomes that integrate and excise the genome of phage
174 ation, and will allow modelling of the HIV-1 intasome to aid in the development of antiretroviral dru
175 e nucleoprotein structure, which we term the intasome to distinguish it from the greater preintegrati
176 these contacts impinge on the ability of the intasome to engage nucleosomes in vitro and redistribute
177 s repurposed as an integral component of the intasome to mediate HTLV-1 integration.
178 nd BET proteins for MoMLV) that tether viral intasomes to chromatin.
179 forms a higher order nucleoprotein assembly (intasome) to catalyze the integration reaction, in which
180 ving rise to both tetrameric and dodecameric intasomes, together with other less well-characterized s
181                             Although several intasome/transpososome structures from the DDE(D) recomb
182     Under suboptimal assembly conditions for intasomes using U3 att DNA, DNase I probing demonstrated
183 mes, although the number of protomers in the intasome varies between viruses.
184 tion of IN at the att sites in reconstituted intasomes was similar to that observed in PIC.
185 otein-DNA interactions within the functional intasome were lacking.
186  outer CTDs contribute to aggregation of PFV intasomes which may be inhibited by high ionic strength
187 into a recombinationally active complex, the intasome, which is capable of attB capture and formation
188 he structure of the cleaved synaptic complex intasome, which is the direct target of INSTIs.
189 ttL, producing SarA-DNA complexes similar to intasomes, which consist of bacteriophage lambda integra
190 contained tetramers but failed to produce an intasome with octamers.
191 etically stabilized Rous sarcoma virus (RSV) intasomes with human immunodeficiency virus type 1 stran

 
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