ライフサイエンスコーパス: intercalator

1 ow that, in fact, carbamazepine is not a DNA intercalator.

2 ution, indicating that the complex is not an intercalator.

3 al DNA through photoreactions with a rhodium intercalator.

4 base pair rise accommodates stacking of the intercalator.

5 y the groove and sequence recognition of the intercalator.

6 which suggests that NSC 314622 is not a DNA intercalator.

7 st binding affinity reported for such a mono-intercalator.

8 d 3D self-assembled NP lattices by molecular intercalator.

9 e DNA-binding behaviour of a threading tetra-intercalator.

10 scent probe and stained nonspecifically with intercalator.

11 uplex that selectively binds a complementary intercalator.

12 ence of any DNA aggregation by the DNA-bound intercalators.

13 of some of the most effective anticancer DNA intercalators.

14 ents, makes them potential candidates as DNA intercalators.

15 ng ethidium bromide or SYBR Green I as model intercalators.

16 rapid electron transfer between redox active intercalators.

17 t closely resembles cytotoxic antineoplastic intercalators.

18 top2, unwound DNA, and are assumed to be DNA intercalators.

19 tivated DNA cleavage assays with the rhodium intercalators.

20 significantly different from those caused by intercalators.

21 ine analogs do not "behave" like classic DNA intercalators.

22 nteractions between caffeine and various DNA intercalators.

23 riven binding through strategic selection of intercalators.

24 ng a new paradigm for rational design of DNA intercalators.

25 now tested the hypothesis that the membrane intercalator 1-hexadecanol (HD) similarly associates wit

26 In the absence of any bis-intercalators, 7-9 nucleotides (nt) in each of the stran

27 ric acids (8-oxoxanthine) with the known DNA intercalator acridine orange (AO).

28 lexation between caffeine and the planar DNA intercalator acridine orange.

29 a triple-helical structure stabilized by an intercalator, acridine, attached to the third strand of

30 These results support the hypothesis that intercalators (acting as 'molecular midwives') could hav

31 alation site for binding by the GC-selective intercalator actinomycin D (ActiD).

32 GC-specific intercalator actinomycin D and three minor groove-binder

33 se reactions are performed in the absence of intercalators, almost exclusively cyclic tetra- and octa

34 ssumption that other charged, pi-stacked DNA intercalators also have approximately these same values,

35 The three intercalators also protected the red cells against lysis

36 f a macrocyclic bisacridine and an antitumor intercalator ametantrone to DNA has been studied.

37 CP-115,953, etoposide, and azatoxin and the intercalators amsacrine and mitoxantrone.

38 These results were compared with a spermine-intercalator analogue.

39 es, covalently crosslinked to a redox-active intercalator and containing the binding site of the test

40 leotides synthesized with a tethered rhodium intercalator and guanine doublets placed before and afte

41 several agents, including adriamycin (a DNA intercalator and inhibitor of topoisomerase II), camptot

42 on complementary stereochemistry between bis-intercalator and junction.

43 wn by comparison with reactions of a rhodium intercalator and methylene blue, respectively.

44 ation occurs in both directions from the NDI intercalator and on both DNA strands of the target, but

45 ff by collisional quenching between adjacent intercalator and reporter dyes.

46 lization, and by comparative analyses of the intercalator and salt induced release from the nucleosom

47 The methoxyacrylyl-anthranilate intercalator and the minor groove binding A-B-C+ risacch

48 d we provide data showing that DJ34 is a DNA intercalator and topoisomerase II inhibitor.

49 or atypical DNA recognition via multiple DNA intercalators and a structural basis for the drugs' pote

50 Conventional mono-intercalators and bis-intercalators are discussed, follo

51 ction analyses of the complexes between both intercalators and CGTACG.

52 ed xanthine, interacts with polyaromatic DNA intercalators and has been hypothesized to interfere wit

53 dergo conformational changes when exposed to intercalators and may be of relevance when exploring how

54 in creating an artificial nuclease with DNA intercalators and metallopeptides.

55 onspecific binders, and we demonstrated that intercalators and minor groove binders affect the confor

56 (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism.

57 La nuclei occurs upon treatment with rhodium intercalators and photoactivation.

58 t DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentratio

59 on that electrostatic attraction between the intercalators and the base pairs in the cleavage complex

60 d macrocyclic diimine and triaza DNA-binding intercalators and their platinum(II) conjugates have bee

61 of structural complexity relative to simple intercalators, and can provide significant insights into

62 Bisnaphthalimide intercalators are anti-tumour agents composed of two pla

63 Although DNA intercalators are believed to inhibit DNA polymerases an

64 Conventional mono-intercalators and bis-intercalators are discussed, followed by unconventional

65 nteractions between those two classes of bis-intercalators are quite different.

66 thesized ferrocenyl (Fc) double-stranded DNA intercalator as a redox marker is presented.

67 his site, consistent with the effect of some intercalators as suppressors of Top2-mediated DNA cleava

68 ous chemical DNA modifications and molecular intercalators as tools for the manipulation of DNA-nanop

69 constructed that contain a tethered rhodium intercalator, as photooxidant, as well as two 5'-GG-3' s

70 mer does not diminish oxidation by a Ru(III) intercalator at a distal guanine doublet, suggesting tha

71 o-tide conjugate was prepared having the NDI intercalator at both the 5'- and 3'-termini.

72 The proposed intercalator-based strategy permits the creation of reco

73 ly establish the advantage of bis- over mono-intercalators before demonstrating the approach by visua

74 Threading intercalators bind DNA with high affinities.

75 DNA intercalators bind nucleic acids by stacking between adj

76 mers also reveal a surprising sensitivity of intercalator binding enthalpy and entropy to the details

77 hat within this set of sequence specific bis-intercalators, both DNA grooves can be accessed, setting

78 The signals obtained from redox-active intercalators bound to DNA-modified gold surfaces displa

79 ontrast to the semi-disordered state of mono-intercalators bound to the same DNA molecule.

80 istently describe the mechanical behavior of intercalator-bound DNA.

81 de (EthBr) is the cheapest and most used DNA intercalators, but presents carcinogenic and teratogenic

82 However, one bis-intercalator--but not its sister with a shorter linker--

83 These applications illustrate how intercalators can be used to facilitate and expand DNA-b

84 Other studies have demonstrated how intercalators can be used to promote the formation of ot

85 Collectively, our results show that DNA intercalators can trigger a common mitochondrial respons

86 r (involving the 222 symmetry) such that the intercalator cavity is flanked by two sets of G x C base

87 The interbend DNA was unwound by use of the intercalator chloroquine, or, alternatively, by raising

88 g the dynamic and equilibrium aspects of DNA-intercalator complex assembly may allow optimization of

89 ting dissociation rate constant of the tetra-intercalator complex dissociating from its preferred bin

90 which the peptide, the metal cation, and the intercalator components of the conjugate are each varied

91 Many different intercalator compounds have been studied because of thei

92 Using intercalator-conjugated AG motif TFOs, we compared the e

93 The results may be useful in the design of intercalator-conjugated oligonucleotides for antisense a

94 nding properties of EDTA.Fe-modified peptide-intercalator conjugates (PICs) are described.

95 g affinity and specificity of aminoglycoside intercalator conjugates can be tuned by optimizing the l

96 rea as a carrier group in cytotoxic platinum-intercalator conjugates prompted us to explore the synth

97 Importantly, the polyamide-intercalator conjugates, which combine sequence-specific

98 Although many natural intercalators contain multiple chiral subunits, only int

99 taarginine units are appended onto a rhodium intercalator containing the sterically expansive chrysen

100 lization) or by adding the triplex selective intercalator coralyne (1000-fold stabilization).

101 n, as did the use of the triplex-stabilizing intercalator coralyne.

102 ffer supplemented with the triplex selective intercalator coralyne.

103 r in the presence of the triplex-stabilizing intercalator coralyne.

104 An intercalator coupled with a de novo-designed alpha helix

105 signal of methylene blue, a redox-active DNA intercalator, coupled to [Fe(CN)6]3-.

106 ic DNA duplexes were prepared with a rhodium intercalator covalently attached to one end and separate

107 DNA hybridization by using the electroactive intercalator daunomycin (DM).

108 All the intercalators decreased accessibility throughout the str

109 dine ring system render these PICs threading intercalators, directing the substituents into both groo

110 A generalized variant of a fluorescent intercalator displacement (FID) assay conducted on a ser

111 se of a technically nondemanding fluorescent intercalator displacement (FID) assay for initial assess

112 o that of distamycin A, using a fluorescence intercalator displacement (FID) assay.

113 sequence selectivity using this fluorescent intercalator displacement (FID) assay.

114 Fluorescent intercalator displacement (FID) is a convenient and prac

115 ing the existing high-throughput fluorescent intercalator displacement (HT-FID) assay developed by Bo

116 A generalized variant of a fluorescent intercalator displacement assay conducted on a series of

117 ed by competition dialysis and a fluorescent intercalator displacement assay.

118 es, allowing the elaboration of a variety of intercalator displacement assays and label-free biosenso

119 The 'fluorescent intercalator displacement replacement' (FIDR) assay is i

120 DNase I footprinting, fluorescence intercalator displacement studies, and circular dichrois

121 ), CD (circular dichroism), FID (fluorescent intercalator displacement), and UV (ultraviolet) thermal

122 , we have reported the NMR analysis of a bis-intercalator-DNA complex in which the peptide linker bet

123 n the same range observed for binding of the intercalators doxorubicin and pyrazoloacridine to DNA.

124 s then enhanced through coordinated designer intercalators-DPQ and DPPZ-and high-precision DNA cleava

125 f a bulged DNA site due to the binding of an intercalator drug may perturb the recognition of DNA def

126 also tested positive for DNA in fluorescent intercalator dye and whole genome amplification (WGA) as

127 alation (FIT)-probes, in which two (or more) intercalator dyes of the thiazole orange (TO) family ser

128 somerase II (TOP2) can be interrupted by DNA intercalators (e.g., doxorubicin), enzyme binders (e.g.,

129 Invader probes based on large intercalators efficiently recognize model targets.

130 ns > 10,000-fold greater than those at which intercalators enable polymerization.

131 We found that substitutions closer to the intercalator end of the TFO (positions 4-6) had a more d

132 Finally, intercalators enhance topoisomerase I-mediated cleavage

133 ossible by the stabilisation provided by the intercalator, enhances mismatch discrimination.

134 ns containing caffeine and the nonplanar DNA intercalator ethidium bromide show optical shifts and st

135 lkaloids berberine and palmatine and the DNA intercalator ethidium bromide, revealed a change in the

136 tures of the two oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and mi

137 Standard intercalators (ethidium, daunorubicin, and actinomycin D

138 The classic DNA intercalator, ethidium, was used to probe the effects of

139 Ru(phen)(bpy')(dppz)(2+), and three organic intercalators, ethidium (Et), thionine (Th), and anthraq

140 coli top2 enzymes in the presence of the non-intercalators fluoroquinolone CP-115,953, etoposide, and

141 iation of 32P-labeled duplex DNA with either intercalator followed by denaturing PAGE allows the dete

142 Ethidium bromide has served as a classic DNA intercalator for more than four decades.

143 een cholesterol and endogenous and exogenous intercalators for association with membrane phospholipid

144 Intercalator-free polymerization is not observed, even a

145 -intercalator-functionalized uridine and N2'-intercalator-functionalized 2'-N-methyl-2'-aminouridine

146 modification with +1 interstrand zippers of intercalator-functionalized nucleotides such as 2'-O-(py

147 e, and +1 interstrand zipper arrangements of intercalator-functionalized nucleotides, respectively.

148 be short synthetic routes to a series of O2'-intercalator-functionalized uridine and N2'-intercalator

149 ves including graveolinine and a triplex DNA intercalator have been synthesized by using the catalyti

150 uss how DNA modifications and small molecule intercalators have been employed in the chemical and pho

151 ker length, and surface area of the appended intercalators have marked impact on DNA-hybridization ch

152 In contrast, an alkylating intercalator hedamycin strongly inhibited binding of bot

153 properties of Invader probes based on larger intercalators, i.e., perylene and coronene, expecting th

154 ining an acridine ring system, the strongest intercalator in the series, gave a small but clearly det

155 ity change (DeltaC(p)()) for a series of DNA intercalators, including ethidium, propidium, daunorubic

156 ng an oxaliplatin prodrug and a cationic DNA intercalator inhibit the growth of TMZ-resistant cells f

157 nd to induce nucleolar stress, including DNA intercalators, inhibitors of mTOR/PI3K, heat shock prote

158 We show that this model intercalator interacts with duplex DNA by a "displacemen

159 isomerization to the Z isomer transforms the intercalator into a chain capper, allowing dynamic short

160 ting cardiotoxicity in iPSC-CMs included DNA intercalators, ion channel blockers, epidermal growth fa

161 rmini with a naphthalene diimide-based (NDI) intercalator is described.

162 score the fact that the DNA binding of a bis-intercalator is directional and different from the simpl

163 hat delivery of the peptide by the ruthenium intercalator is not essential for cross-linking; peptide

164 by charge transfer from the tethered rhodium intercalator is quantitative, albeit with low photoeffic

165 hat the DNA binding behavior of these simple intercalators is driven almost equally by hydrophobic ef

166 DNA detection, the use of indicators and/or intercalators is necessary to improve electrochemical se

167 Chemotherapy using DNA intercalators is one of the most successful approaches t

168 srupt TOPO II inhibition by the tethered DNA intercalator, it did provide an alternative mode of entr

169 analysis of a complex between a related bis-intercalator known to display altered DNA sequence speci

170 ere synthesized that incorporate a ruthenium intercalator linked covalently to the 5' terminus of one

171 erization is most favored if the size of the intercalator matches that of the base pair; intercalator

172 d COL1A1 expression and suggest that Sp1-DNA intercalators may be an effective and novel approach for

173 a human leukemia cell line, is resistant to intercalator-mediated DNA complex formation and cytotoxi

174 Double strand specific intercalator methylene blue in combination with [Fe(CN)6

175 monstrates that, when administered to cells, intercalators might not only associate with polynucleoti

176 Our study provides insights on intercalators-mismatch DNA interactions and a rationale

177 ses as a function of the surface area of the intercalator moiety.

178 east Top2 conferring hypersensitivity to the intercalator N-[4-(9-acridinylamino)-3-methoxyphenyl]met

179 idation of ascorbic acid (AA) by a threading intercalator (N,N'-bis(3-propylimidazole)-1,4,5,8-naphth

180 Conversely, the membrane intercalator, n-octanol, increased cholesterol oxidation

181 ied ligands, including pyrrole polyamide and intercalator natural products, designed hairpin pyrrole-

182 matically (>12 degrees C) in the presence of intercalator-neomycin conjugates (1-4) even at a very lo

183 The binding preference of intercalator-neomycin conjugates (1-4) to poly(dA).2poly

184 odynamic studies on the interactions between intercalator-neomycin conjugates and a DNA polynucleotid

185 ed here suggest that the binding affinity of intercalator-neomycin conjugates for poly(dA).2poly(dT)

186 The thermodynamic parameters of these intercalator-neomycin conjugates with poly(dA).2poly(dT)

187 In contrast to intercalator-neomycin conjugates, the increment of T(m3-

188 also explored, but with a mismatch-specific intercalator, no site-specific hydrolysis was found.

189 ultaneous addition of both EGR1 and TBP, the intercalator nogalamycin prevented EGR1 complex formatio

190 contrast, although they are also DNA-binding intercalators, none of the amide-functionalized anthrace

191 lyethylene glycol (M(W)~2000 Da) capped with intercalators of varying hydrophobicity, charge, and siz

192 e study the effects of position-specific DNA intercalators on the rate and extent of single-turnover

193 the impact of an additive (chain capper and intercalator) on the chain length over a range of concen

194 ped spirocyclic molecule, DDI (double-decker intercalator), on the basis of our earlier work with the

195 a glioma cell line treated with the cationic intercalator or with TMZ showed substantial differences

196 l method involving elution of histones using intercalators or salt, to assess stability features depe

197 DNA crosslinkers (ultraviolet light) and DNA intercalators (oxaliplatin or adriamycin).

198 sites needs to be considered when estimating intercalator parameters from zero-stress elongation and

199 Quinomycin A, a bis-intercalator peptide, has previously been shown to targe

200 d plasmid has also been demonstrated with an intercalator-peptide conjugate containing acidic residue

201 copper, have been delivered to DNA with our intercalator-peptide conjugates to effect oxidative chem

202 o DNA, and the binding constants for several intercalator-peptide conjugates were determined by lumin

203 An unusual intercalator platform is formed by bringing four complex

204 The conventional wisdom argues that DNA intercalators possess a condensed polyaromatic ring wher

205 2D- and 3D-DNA systems, we demonstrate that intercalators present a powerful alternative for the adj

206 e neighbor-exclusion model, in which a bound intercalator prevents intercalation into adjacent sites.

207 hate (GMP) with an azobenzene-containing DNA intercalator produces supramolecular crystals arranged t

208 Additionally, the rhodium intercalators promote strand cleavage at the mismatch si

209 the MutY binding site using tethered rhodium intercalators promoted cross-linking and yielded informa

210 Fluorescence of the DNA-bound intercalator provides information on the molecule length

211 ty of the duplexes, electrochemical data for intercalator reduction on different mismatch-containing

212 Dumbbell-shaped DNA threading intercalators represent the next order of structural com

213 topo IIbeta expression may contribute to the intercalator resistance of HL-60/AMSA cells.

214 A photoexcited rhodium intercalator, Rh(phi)2DMB3+ (phi = 9,10-phenanthrenequin

215 The intercalator, [Rh(phi)(2)bpy']Cl(3) [phi = 9,10-phenanth

216 Here we show the multi-intercalator [Ru(dppz)2(PIP)](2+) (dppz = dipyridophenaz

217 We also characterized the flexibility of intercalator-saturated dsDNA for the first time.

218 Several of the intercalators show enhanced cytotoxicity compared to pro

219 ng isoamyl alcohol extractions combined with intercalator-specific spin columns, thereby yielding the

220 Sterically demanding intercalators such as [Rh(bpy)2(chrysi)]3+ may have appl

221 rase in mammalian cells, is inhibited by DNA intercalators, such as doxorubicin, in an in vitro assay

222 Here, we propose the use of simple DNA intercalators, such as ethidium bromide, as tools to fac

223 ltured cells, we show that DXR and other DNA intercalators, such as ethidium bromide, can rapidly int

224 lts may in turn guide the rational design of intercalators synthesized for DNA-targeted drugs, optica

225 of novel derivatives of the quinoxaline bis-intercalator TANDEM.

226 Bulky rhodium intercalators target DNA base mismatches with high speci

227 has been designed as a sterically demanding intercalator targeted to destabilized mismatched sites i

228 Our study suggests that improved XR5944 bis-intercalators targeting ERE may be designed through opti

229 n initiation and the utility of the platinum-intercalator technology for the design of sequence-speci

230 meric sequence nonspecifically, the platinum-intercalator technology has the potential to produce tel

231 range (16 to 26 angstroms), with the rhodium intercalator tethered to either end of the duplex assemb

232 ; pluramycin, a minor-major-groove threading intercalator that alkylates N7 of guanine; KMnO4, which

233 free energy landscape for an unconventional intercalator that binds DNA through a novel two-step mec

234 (chrysi)]3+ is a novel, sterically bulky DNA intercalator that has been designed to bind specifically

235 Doxorubicin is an anthracycline DNA intercalator that is among the most commonly used antica

236 ochondria after photoreaction with a rhodium intercalator that penetrates the intact mitochondrial me

237 intercalator matches that of the base pair; intercalators that bind to Watson-Crick base pairs promo

238 DNA intercalators that have high affinity and slow kinetics

239 Two intercalators that present efficient FRET were used to e

240 Here we examined the effect of two DNA intercalators that prevent Sp1 binding on the expression

241 results identify specific binding sites for intercalators that result in trapping of Top2.

242 They are DNA intercalators that upon photoexcitation form adducts wit

243 and structural similarity to a known triplex intercalator, the napthylquinoline MHQ-12.

244 The intercalator (thiazole orange or benzothiazole orange) p

245 ctrochemical data monitoring reduction of an intercalator through DNA films, damage ratios correlate

246 e utilize ethidium bromide (EtBr) as a model intercalator to demonstrate selective and isotropic latt

247 The binding of the Fc intercalator to dsDNA was measured by differential pulse

248 h permits access of the sterically demanding intercalator to the base stack.

249 The binding of intercalators to an Okazaki hairpin of sequence 5'-r(gc)

250 Recent studies have used DNA scaffolds and intercalators to construct supramolecular assemblies tha

251 relevant in understanding binding of certain intercalators to DNA structure associated with the quadr

252 we describe the application of bulky rhodium intercalators to inhibit cellular proliferation differen

253 The results show that the binding of intercalators to Okazaki fragments is accompanied with h

254 base pair-to-dye ratios of 10/1 for the bis-intercalator TOTO-1 and 5/1 for YOYO-1 for all three loc

255 h locus were mixed individually with the bis-intercalators TOTO-1 and YOYO-1 and their corresponding

256 The diverse properties of DNA intercalators, varying in affinity and kinetics over sev

257 Variation of the rhodium intercalator was also explored, but with a mismatch-spec

258 The NDI intercalator was prepared in a single step from the corr

259 In contrast to other bis-intercalators, we find that ligand association is descri

260 NDI and octahedral rhodium intercalators, when tethered directly to the 5' terminus

261 ear decrease in current flow for a DNA-bound intercalator, where the reaction is DNA-mediated; in con

262 robust DNA structural recognition of chiral intercalators, which are critical for optimizing DNA bin

263 important class of these ligands is that of intercalators, which bind DNA by inserting aromatic moie

264 or groove binding ligands, and then focus on intercalators, which have been probed extensively with t

265 interact with the phenanthroline ring of the intercalators, which may lead to a larger disruption of

266 ncer agents, including camptothecins and DNA intercalators, which reversibly stabilize (trap) top1 ca

267 The NDI intercalator with a free hydroxyl was then added in the

268 effect may be due to complexation of the DNA intercalator with caffeine.

269 ntiplasmodial activity, but it is also a DNA intercalator with cytotoxic properties.

270 XR5944, a deoxyribonucleic acid (DNA) bis-intercalator with potent anticancer activity, can bind t

271 thracycline drug nogalamycin, a non-covalent intercalator with preference for 5'-TG dinucleotides, in

272 d by the binding of ditercalinium, a DNA bis-intercalator with strong anti-tumour properties.

273 ligands based on the derivatization of this intercalator with two neamine moieties (Amt-Nea,Nea) or

274 ators contain multiple chiral subunits, only intercalators with a single chiral unit have been quanti

275 Several intercalators with different complexities, including eth

276 In contrast, the related bis-intercalator WP652 prefers the 5'-PyGTPu tetranucleotide

277 nce intensity response was obtained with the intercalator YOYO-1 using allele 8 of the F13A01 locus,