ライフサイエンスコーパス: involucrin

1 cteristic of its final state (keratin 14 and involucrin).

2 atinocyte differentiation markers (loricrin, involucrin).

3 g later markers (profilaggrin, loricrin, and involucrin).

4 nnexin 43 (Cx43), cytokeratin K10 (K10), and involucrin.

5 lacement of K10 and increasing expression of involucrin.

6 ed keratins 1 and 6, filaggrin, loricrin and involucrin.

7 nd gain in a terminal differentiation marker involucrin.

8 pression of late-stage markers, loricrin and involucrin.

9 nified envelope proteins, most abundantly to involucrin.

10 ast cells, or in the epidermal expression of involucrin.

11 d the expression of loricrin, filaggrin, and involucrin.

12 turation were assessed by immunostaining for involucrin.

13 entiation-associated target genes, including involucrin.

14 activity of the differentiation marker gene, involucrin.

15 of the cornified envelope precursor protein involucrin.

16 shed CE precursor proteins SPR1A, SPR1B, and involucrin.

17 nd DNMT3B and negatively with p16(INK4A) and involucrin.

18 differentiation, as assayed by expression of involucrin.

19 ers of epithelial differentiation, including involucrin.

20 and mRNA levels of filaggrin, loricrin, and involucrin.

21 creased expression of the structural protein involucrin.

22 ssion of the suprabasal markers loricrin and involucrin.

23 fferentiation markers such as keratin 10 and involucrin.

24 e SC and enhanced expression of loricrin and involucrin.

25 of differentiation-associated keratin 10 and involucrin.

26 elium and express the differentiation marker Involucrin.

27 the adult vagina, E(2) induced expression of involucrin, a CCAAT/enhancer-binding protein beta and cy

28 Involucrin, a cornified envelope precursor, and the cros

29 related with a decrease in the expression of involucrin, a differentiation marker.

30 es of keratin 5, a proliferative marker, and involucrin, a differentiative marker, respectively.

31 explore the impact of KLF4 on expression of involucrin, a gene that is specifically expressed in dif

32 Involucrin, a marker of epithelial differentiation, and

33 er activity and endogenous protein levels of involucrin, a marker of keratinocyte terminal differenti

34 ophoretic mobility supershift assay using an involucrin activator protein 1 (AP1) response element se

35 Involucrin also binds to SLV containing 12-18% phosphati

36 f differentiation and in contrast to that of involucrin, an early marker of terminal differentiation,

37 ncestral glutamine-glutamate-rich regions of involucrin, an important CE structural protein.

38 arize the literature regarding regulation of involucrin, an important marker gene that serves as a mo

39 etween specific glutaminyl residues of human involucrin and a synthetic analog of epidermal specific

40 Using involucrin and an epidermal omega-hydroxyceramide analog

41 y of the VDRE promoter and the expression of involucrin and CYP24 mRNA.

42 osition of membranes, transglutaminases, and involucrin and envoplakin in the initiation of CE assemb

43 containing lipid Z, lipid Z was attached to involucrin and formed saponifiable protein-lipid adducts

44 ydrocortisone in vitro induced expression of involucrin and high-molecular-mass CKs that are characte

45 his was featured by decreased E-cadherin and involucrin and increased vimentin and integrin beta(1).

46 20(OH)D3 stimulated involucrin and inhibited cytokeratin 14 expression.

47 ed expression of the differentiation markers involucrin and keratin 10 compared to cells with no cell

48 ssion pattern of the differentiation markers involucrin and keratinocyte transglutaminase.

49 lls at the apical surface; the expression of involucrin and keratins 6, 13, 14, and 19; and the absen

50 melatonin and AFMK-stimulated expression of involucrin and keratins-10 and keratins-14 in the epider

51 rneum junction and a modest decrease in both involucrin and loricrin protein expression, markers of k

52 ged, whereas the cornified envelope proteins involucrin and loricrin were increased in ft/ft epidermi

53 ecreased expression of K1 and K10 but not of involucrin and loricrin.

54 affected in nkt skin, with overexpression of involucrin and profilaggrin/filaggrin along with focal a

55 cornified envelope (CE) precursor proteins (involucrin and small proline-rich [Sprr] -1a, -1b, -2a,

56 quamation of and cornified envelope protein (involucrin and small proline-rich protein [SPRR]-2) expr

57 es for lipid synthesis and the expression of involucrin and small proline-rich proteins, which covale

58 , improved corneal smoothness, and decreased involucrin and SPRR-2 immunoreactivity compared with EDE

59 stress significantly increased expression of involucrin and SPRR-2 in the corneal epithelia.

60 We propose a model in which involucrin and TGase 1 bind to membranes shortly after e

61 However, on SLV carrying both involucrin and TGase 1, only five glutamines serve as do

62 glitazone, a PPAR-gamma activator, increases involucrin and transglutaminase 1 mRNA levels.

63 mRNA and protein levels of involucrin and transglutaminase 1, markers of differenti

64 rotein levels of the differentiation markers involucrin and transglutaminase following administration

65 Levels of involucrin and transglutaminase mRNA and protein were in

66 ation of the calcium-stimulated increases in involucrin and transglutaminase mRNA levels.

67 substantial reduction in calcium-stimulated involucrin and transglutaminase promoter activities.

68 The results showed that involucrin and transglutaminase protein and mRNA levels

69 e also activators of PPARalpha, also induced involucrin and transglutaminase protein and mRNA.

70 of the keratinocyte differentiation markers involucrin and transglutaminase to 1,25-dihydroxyvitamin

71 While induction of the spinous layer markers involucrin and transglutaminase was compatible with late

72 ed expression of the differentiation markers involucrin and transglutaminase were also blocked by the

73 , as demonstrated by increased expression of involucrin and transglutaminase, and inhibited prolifera

74 tion of keratinocyte differentiation markers involucrin and transglutaminase.

75 nly one lysine and two glutamine residues of involucrin and two glutamines of envoplakin were used in

76 inocyte differentiation (cytokeratin K10 and involucrin) and markers of apoptosis (TUNEL and anticasp

77 dance of epidermal barrier proteins (FLG and involucrin) and prevented cytokine-mediated stratum corn

78 n epithelial structural integrity (e.g. Ivl (involucrin) and Sbsn (suprabasin)).

79 f epithelial differentiation marker protein (involucrin), and change of cell cycle position.

80 inocyte differentiation (cytokeratin K10 and involucrin), and markers of apoptosis (TdT-mediated dUTP

81 f the squamous differentiation markers Spr1, involucrin, and cytokeratin 1.

82 litazone or troglitazone increases loricrin, involucrin, and filaggrin expression without altering ep

83 rabasal keratin 10, transglutaminase type I, involucrin, and filaggrin.

84 XA7 expression activated transglutaminase 1, involucrin, and keratin 10 message and protein levels, d

85 differentiation markers, such as filaggrin, involucrin, and loricrin, was slightly increased in PPAR

86 ssion of one of these cross-linked proteins, involucrin, and that this effect can be abolished by mut

87 elium is stratified and is positive for K14, involucrin, and TRP63, but negative for keratin 10.

88 Ca2+-sensitive involucrin AP-1 promotor activity was increased, both in

89 Filaggrin (FLG), loricrin (LOR), and involucrin are important epidermal barrier proteins.

90 ed that key differentiation genes, including involucrin, are bound to heavy polysomes during differen

91 entiation proteins, filaggrin, loricrin, and involucrin, became abnormal.

92 Preferential involucrin biotinylation and the increased cornified cel

93 pression and induce differentiation markers (Involucrin, CK10) in OSCC 3D cultures.

94 It resembles the involucrin coding region of other non-anthropoid mammals

95 rentiating agent on the promoter activity of involucrin, consistent with promotion of early different

96 fundibula expressed filaggrin, profilaggrin, involucrin, cornifin alpha, and loricrin.

97 GF-2, IFNalpha2, IL-1RA, HSA, keratin-6, and involucrin; cortisol was significantly higher (p < 0.05)

98 wever, to date, direct isolation from CEs of involucrin cross-linked by way of the transglutaminase-i

99 sequencing revealed many peptides involving involucrin cross-linked either to itself or to a variety

100 08 and glutamines 465 and 489 for interchain involucrin cross-links.

101 cies represents an early intermediate in the involucrin crosslinking process.

102 ntiation marker keratin K1 and inhibition of involucrin crosslinking.

103 neage-tracing studies in combination with an involucrin-driven Cre/lox reporter system confirmed that

104 Involucrin, envoplakin, and periplakin form the protein

105 In CEs of 3-d cultured cells, involucrin, envoplakin, and small proline-rich proteins

106 Together, these data indicate that involucrin, envoplakin, periplakin, and possibly other s

107 barrier proteins-envoplakin, periplakin, and involucrin (EPI-/- mice)-have a defective cornified laye

108 barrier proteins-envoplakin, periplakin and involucrin-(EPI-/- mice) have a defective cornified laye

109 on microscopy, this exposed large amounts of involucrin epitopes as well as of desmoplakin, a desmoso

110 ts keratinocyte differentiation, we assessed involucrin expression in HHD keratinocytes.

111 Involucrin expression is tightly linked to the onset of

112 orbol-13-acetate-dependent increase in human involucrin expression, and PRMT5 dimethylates proteins i

113 ed for appropriate differentiation-dependent involucrin expression, and that the mechanism of regulat

114 ng the action of 1,25-dihydroxyvitamin D3 on involucrin expression, but the vitamin D response elemen

115 alcium or vitamin D-induced up-regulation of involucrin expression, suggesting that the enzymatic act

116 to the timing and requirements for Aire and involucrin expression, the latter a marker of terminally

117 augments the PKCdelta-dependent increase in involucrin expression, whereas KLF4 knockdown attenuates

118 tosidase staining follows that of endogenous involucrin expression.

119 region of the promoter required for in vivo involucrin expression.

120 scriptionally inactive forms do not increase involucrin expression.

121 y inhibition of calcium or vitamin D-induced involucrin expression.

122 tern-regulated cell-cell contact to modulate involucrin expression.

123 by pattern size, did not alter keratinocyte involucrin expression.

124 anscription factors known to be required for involucrin expression.

125 including decreased keratin-14 and increased involucrin expression.

126 ent of the epidermis revealed a reduction in involucrin, filaggrin, and loricrin-markers of different

127 P63, C/EBPdelta, CK10 and involucrin fluorescence combined with morphology observa

128 The vitamin D response element from the involucrin gene bound the vitamin D receptor and the ret

129 Regulation of human involucrin gene expression and promoter activity was ass

130 factors and signaling cascades in regulating involucrin gene expression are presented.

131 y region AP1-5 element, in the regulation of involucrin gene expression during corneal epithelial cel

132 ut is absolutely necessary for activation of involucrin gene expression in the differentiating cornea

133 Involucrin gene expression is initiated early in the dif

134 In conclusion, calcium-regulated involucrin gene expression is mediated at least in part

135 urcumin and EGCG produce opposing effects on involucrin gene expression via regulation of C/EBP facto

136 Regulation of involucrin gene expression was monitored in cultures of

137 n promoter or prevent calcium stimulation of involucrin gene expression, but blocked 1,25-dihydroxyvi

138 the impact of specific promoter mutations on involucrin gene expression.

139 and proximal-regulatory regions, to regulate involucrin gene expression.

140 regulation of human corneal epithelial cell involucrin gene expression.

141 omplete differentiation-dependent program of involucrin gene expression.

142 ducer of keratinocyte differentiation and of involucrin gene expression.

143 The coding region of the involucrin gene of Tupaia glis has been cloned and seque

144 Previous studies show that the human involucrin gene promoter has two distinct regulatory reg

145 ining HSEs and STREs that are present in the involucrin gene promoter.

146 In the present study, the involucrin gene was used as a model to study this regula

147 within the distal 5'-flanking region of the involucrin gene which contributes to differentiation-dep

148 ncreased AP-1-dependent transcription of the involucrin gene, an effect that may be mediated by liver

149 al promoter activity and TPA response of the involucrin gene.

150 ssion, and transcriptional regulation of the involucrin gene.

151 of the consensus sequence of four mammalian involucrin genes comprises four pairs of complementary o

152 popeptides, the ester linkage formation used involucrin glutamine residues 107, 118, 122, 133, and 49

153 This cascade activates transcription of involucrin (hINV) and other genes associated with differ

154 delta (PKCdelta) is a key regulator of human involucrin (hINV) gene expression and is regulated by ty

155 ) isoforms are important regulators of human involucrin (hINV) gene expression during keratinocyte di

156 ein kinase C (PKC), Ras, and MEKK1 regulates involucrin (hINV) gene expression in epidermal keratinoc

157 ggest that a PKC/Ras/MEKK1 cascade regulates involucrin (hINV) gene expression in human epidermal ker

158 te differentiation as measured by effects on involucrin (hINV) gene expression.

159 In the present study, the human involucrin (hINV) gene was used as a model to study gene

160 expression of the AP1 factor-regulated human involucrin (hINV) gene.

161 Human involucrin (hINV) is a keratinocyte protein that is expr

162 Involucrin (hINV) is a marker of keratinocyte differenti

163 Human involucrin (hINV) is a precursor of the keratinocyte cor

164 Human involucrin (hINV) is a structural protein that is select

165 Human involucrin (hINV) is a structural protein that is select

166 Involucrin (hINV) is an important structural component o

167 Human involucrin (hINV) mRNA level and promoter activity incre

168 The KLF4 induction of human involucrin (hINV) promoter activity is mediated via KLF4

169 s, increased binding of these factors to the involucrin (hINV) promoter, and increased expression.

170 factors suppress transcription of the human involucrin (hINV) promoter.

171 Expression of involucrin (hINV), a marker of keratinocyte differentiat

172 Human involucrin (hINV), first appears in the cytosol of kerat

173 of the keratinocyte differentiation marker, involucrin (hINV), via a Ras, MEKK1, MEK3, p38delta sign

174 skin keratinocytes releases several discrete involucrin-immunoreactive peptides.

175 Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs.

176 Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs.

177 ed decreased expression of Krt86, Krt6b, and involucrin in the epidermal portion of the claw field in

178 mRNA levels for loricrin, profilaggrin, and involucrin in the outer epidermis, but protein levels di

179 ne expression driven by a cellular promoter (involucrin) inserted in an internal position in the retr

180 s required for cornified envelope formation, involucrin (INV) and transglutaminase, increased 2- to 3

181 otein levels of transglutaminase (TGase) and involucrin (INV) over time in culture.

182 pression of Cx26 from the epidermis-specific involucrin (INV) promoter (INV-Cx26) demonstrated that d

183 y to basal, proliferating keratinocytes; the involucrin (Inv) promoter targeted the receptor to supra

184 Involucrin is a major protein of the cornified envelope

185 Involucrin is a major protein of the cornified envelope

186 Involucrin is a marker of human keratinocyte differentia

187 Involucrin is a marker of keratinocyte terminal differen

188 Involucrin is a protein that makes up the cornified enve

189 ucrin provide experimental confirmation that involucrin is an important early scaffold protein in the

190 Involucrin is an integral component of the cornified env

191 Involucrin is expressed in the differentiated suprabasal

192 Although the function and evolution of involucrin is known, the regulation of its gene expressi

193 ggest that when the central segment of human involucrin is predominantly alpha-helical, accompanied b

194 S5 and early differentiation markers such as involucrin (IVL) and cytokeratin CK13 in a CSL-dependent

195 This included genes such as involucrin (IVL), keratinocyte differentiation-associate

196 for epidermal function and are surrounded by involucrin (IVL).

197 Thus, involucrin joins the ranks of a small set of genes that

198 ile of the epidermal differentiation markers involucrin, keratin 10, and filaggrin during tissue reco

199 Both SPRR2 and involucrin levels accumulated in the presence of MDC.

200 were detected in the cytosolic fraction, and involucrin levels increased after UVB.

201 The expressions of involucrin, loricrin, and cathepsin L is initially incre

202 Cytokeratin 10, involucrin, loricrin, and filaggrin protein levels were

203 necessary for cornified envelope formation, involucrin, loricrin, and filaggrin, and the activity of

204 pression of terminal differentiation markers involucrin, loricrin, and filaggrin.

205 oxysterol treatment increased the levels of involucrin, loricrin, and profilaggrin protein and mRNA

206 erexpression and knockdown studies show that involucrin mRNA and protein level correlates directly wi

207 A, in turn, was caused by increased rates of involucrin mRNA degradation.

208 f cornified envelope formation, reduction of involucrin mRNA expression, and transcriptional regulati

209 Our previous studies show that involucrin mRNA levels are increased by the keratinocyte

210 volucrin protein levels were caused by lower involucrin mRNA levels in HHD keratinocytes.

211 t PPARalpha activators induce an increase in involucrin mRNA levels.

212 n of Kdap mRNA expression similar to that of involucrin mRNA, but with differing kinetics.

213 Decreased involucrin mRNA, in turn, was caused by increased rates

214 ntrolled--demonstrates that the emergence of involucrin(+) mTECs critically depends upon the presence

215 Here we demonstrate that both involucrin-negative and involucrin-positive cells are ab

216 6 microm per h) of the mean rate achieved by involucrin-negative cells (46.5 microm per h).

217 in wounds, it is likely that both the basal, involucrin-negative cells and the involucrin-positive su

218 e specific AP-1 proteins, thereby activating involucrin, one of the genes required for epidermal diff

219 holipase C-gamma1 construct showed decreased involucrin or transglutaminase promoter activity in resp

220 Cotransfection of keratinocytes with the involucrin or transglutaminase promoter construct and th

221 erase reporter vectors containing either the involucrin or transglutaminase promoter, the antisense C

222 with a luciferase reporter vector containing involucrin or transglutaminase promoters led to a substa

223 2 had no effect on protein or mRNA levels of involucrin or transglutaminase.

224 Involucrin plays an important role in the lipid and prot

225 Despite their decreased migration rates, involucrin-positive cells appear to possess an intact me

226 emonstrate that both involucrin-negative and involucrin-positive cells are able to respond to a direc

227 The new observation that involucrin-positive cells can indeed migrate suggests th

228 The involucrin-positive cells, however, display mean migrati

229 ltures increased the number of MUC5AC(+) and involucrin-positive cells, which were blocked with the D

230 hannel that was found only in differentiated involucrin-positive cells.

231 the basal, involucrin-negative cells and the involucrin-positive suprabasilar cells respond to this c

232 the basal layer, or the more differentiated, involucrin-positive suprabasilar cells.

233 Epidermal differentiation markers, including involucrin, profilaggrin, and loricrin, detected by immu

234 epidermis there was decreased expression of involucrin, profilaggrin-filaggrin, and loricrin as assa

235 red with the epidermis of PPARalpha+/+ mice, involucrin, profilaggrin-filaggrin, and loricrin express

236 oteins of the upper spinous/granular layers (involucrin, profilaggrin-filaggrin, loricrin) increased

237 ion and distribution of cytokeratin K1, K14, involucrin, proliferating cell nuclear antigen, and p21c

238 knockdown of PKC-eta inhibits TPA-dependent involucrin promoter activation.

239 cholesterol increased transglutaminase 1 and involucrin promoter activity 2- to 3-fold.

240 tentiated the calcium-stimulated increase in involucrin promoter activity unlike NPS S-467 or vehicle

241 PKC-delta knockdown reduces TPA-activated involucrin promoter activity, nuclear activator protein-

242 cribed AP1 sites mediated Whn suppression of involucrin promoter activity.

243 We have sequenced the upstream region of the involucrin promoter and localized a calcium response ele

244 scription factor-binding site present in the involucrin promoter distal regulatory region is required

245 ad epidermis by showing that the full-length involucrin promoter drives differentiation-appropriate e

246 Within the distal regulatory region of the involucrin promoter lies an AP-1 site and an element hom

247 ement did not reduce basal expression of the involucrin promoter or prevent calcium stimulation of in

248 te and the vitamin D response element in the involucrin promoter play important roles in mediating th

249 scription factor DNA binding to two discrete involucrin promoter regions, the distal- and proximal-re

250 e regions in vivo, we have constructed human involucrin promoter transgenic mice and monitored the im

251 The effect of calcium on the involucrin promoter was enhanced synergistically by phor

252 epeat (LTR), the keratin 14 promoter, or the involucrin promoter was not altered, nor was expression

253 on of the -2452 bp to -1880 bp region of the involucrin promoter, or mutation of the AP-1 site within

254 ctivity and block calcium stimulation of the involucrin promoter, whereas the vitamin D response elem

255 The transgenic animals express whn from the involucrin promoter, which is active in keratinocytes un

256 ransfected with either transglutaminase 1 or involucrin promoter-luciferase constructs.

257 , MycERTAM, is targeted to epidermis via the involucrin promoter.

258 vator protein (AP)-1 response element in the involucrin promoter.

259 al keratinocytes of murine epidermis via the involucrin promoter.

260 ne transactivator), expressed from the human involucrin promoter.

261 1,25-dihydroxyvitamin D3 stimulation of the involucrin promoter.

262 1,25-dihydroxyvitamin D3 stimulation of the involucrin promoter.

263 e with desmoglein 3 under the control of the involucrin promoter.

264 press human factor VIII under control of the involucrin promoter.

265 igand-binding domain (ODCER) is driven by an involucrin promoter.

266 cornea tissue, SPRR1, SPRR2, filaggrin, and involucrin protein expression were detected in the centr

267 in mRNA levels, and filaggrin, loricrin, and involucrin protein levels all increased with air exposur

268 +, we found that these cells expressed lower involucrin protein levels at both low and high extracell

269 Decreased involucrin protein levels were caused by lower involucri

270 SPRR2 and involucrin protein levels were studied by immunofluoresc

271 In HCECs, SPRR2 and involucrin proteins were detected in the cytosolic fract

272 The multiple cross-linked partners of involucrin provide experimental confirmation that involu

273 Moreover, cotransfection of the human involucrin reporter plasmid with C/EBPalpha increases pr

274 mmunostaining for keratins 10 and 16 and for involucrin revealed an initial pattern of epithelial imm

275 eratinocyte differentiation regulators (e.g. involucrin, SERPINB7 and SERPINB13), antimicrobial pepti

276 ing of tryptic peptides from TGase 1-reacted involucrin showed a large increase in deamidation of sub

277 l known or novel barrier proteins, including involucrin, small proline-rich proteins, repetin, and ep

278 Nine genes including involucrin, SPRR (types 1A, 1B, 2A, 2B, and 3), late env

279 Levels of involucrin; Sprr-1a, -1b, -2a, -2b, -2f, and -2g; and Tg

280 signaling, continued mTEC development to the involucrin(+) stage maps to activation of the LTalpha-LT

281 g, including keratin 1, loricrin, filaggrin, involucrin, TGK, and SPR-1.

282 ad-to-tail and head-to-head cross-linking of involucrin to itself and to envoplakin and perhaps perip

283 teraction of TGase 1 with substrates such as involucrin to permit specific cross-linking for initiati

284 These results suggest that activation of involucrin transcription involves a pathway that include

285 the promoter for the differentiation marker involucrin, transgenic mice that ectopically express whn

286 le in hair shaft formation (trichohyalin and involucrin, ultra-high sulfur matrix proteins, and trans

287 cytokeratin K1 transglutaminase type I, and involucrin was increased in the absence of exogenous ret

288 The physiological transglutaminase substrate involucrin was preferentially biotinylated in situ, dete

289 d, expression of the EDC genes filaggrin and involucrin was strongly decreased directly by IL-13.

290 line-rich proteins (SPRRs) and filaggrin and involucrin was studied in human cornea sections by immun

291 Involucrin was the first protein to be identified as a l

292 tic digestion after saponification, of which involucrin was the most abundant.

293 n of the differentiation markers K1, K6, and involucrin were abnormal.

294 eratinization-related proteins filaggrin and involucrin were not expressed in normal conjunctival epi

295 When recombinant human TGase 1 and involucrin were reacted on the surface of synthetic lipi

296 By this time >15 residues of involucrin were used for cross-linking.

297 Specific glutamines or lysines of involucrin were used to cross-link the different protein

298 specific cytokeratins (CKs), filaggrin, and involucrin were used to define distinct stages of TE cel

299 EGQLEH, found in the central region of human involucrin, were studied by circular dichroism spectrosc

300 In reactions of involucrin with TGase 1 enzyme in solution, 80 of its 15