ライフサイエンスコーパス: islet

1 d thus insulin secretion) in the rest of the islet.

2 ext of the fully vascularized and innervated islet.

3 n from single and groups of murine and human islets.

4 chronic ER stress in INS-1 cells and rodent islets.

5 of hypothalamic neurospheres and pancreatic islets.

6 n development, in adult brain and pancreatic islets.

7 inantly expressed in beta-cells in mammalian islets.

8 ccumulation and beta cell dysfunction in T2D islets.

9 ccumulation and beta-cell dysfunction in T2D islets.

10 on GSIS in mouse and human control and T2DM islets.

11 indicating dysregulation of lipolysis in T2D islets.

12 eins decreased and one increased versus male islets.

13 xiform layer, Purkinje cells, and pancreatic islets.

14 e increasing Tregs in the remaining inflamed islets.

15 longer than those receiving nonencapsulated islets.

16 d autoreactive immunity toward the engrafted islets.

17 The unique architecture of rodent islets, a so-called core-mantle arrangement seen in two-

18 vel putative biomarkers for early pancreatic islet aberrations preceding T2D.

19 and cytotoxic CD8 T cells appeared early in islets, accompanied by regulatory cells with distinct ph

20 These results support a contribution of the islet-acinar axis in pancreatic development and undersco

21 ) (NSG) mouse model of T-cell-mediated human islet allograft rejection and developed a therapeutic re

22 eatment leads to significant prolongation of islet allograft survival in allosensitized recipients.

23 -2 for 3 weeks significantly prolonged human islet allograft survival.

24 Consequently, a second same-donor islet allograft was rejected in an accelerated fashion b

25 jection and promotes permanent acceptance of islet allografts.

26 rmone islet amyloid polypeptide (IAPP) forms islet amyloid in type 2 diabetes, a process which contri

27 Human Amylin, also known as human islet amyloid polypeptide (hIAPP), is the major factor f

28 f prion protein (PrP(106-126)) and the human islet amyloid polypeptide (hIAPP), with giant lipid vesi

29 The polypeptide hormone islet amyloid polypeptide (IAPP) forms islet amyloid in

30 Amyloid self-assembly of islet amyloid polypeptide (IAPP) is linked to pancreatic

31 he amyloidogenic type II diabetes-associated islet amyloid polypeptide (IAPP), a hydrophobic-hydrophi

32 yphenols against relevant targets, including islet amyloid polypeptide, glucosidases, and cholinester

33 Humans form islet amyloid, but baboon IAPP has not been studied.

34 but also mediate cross talk both within the islet and from islets to other metabolic tissues, thus p

35 l analysis provides more integrated views of islet and pancreatic microcirculation.

36 proximately 50% of alpha-cells in lean mouse islets and 70% of alpha-cells in human islets, suggestin

37 -cell environment in vivo in the db/db mouse islets and ex vivo in C57BL/6J islets exposed to differe

38 hese findings using isolated mouse and human islets and find that the beta cell trophic effect of Wis

39 in secretion, both in vitro in primary human islets and in vivo in human islets transplanted into hig

40 lted in robust T(reg) engraftment within the islets and induced remission in all mice.

41 on leak-mediated NGSIS is conserved in human islets and is stimulated by exposure to nonesterified fr

42 ter transcriptional activity assays in mouse islets and mouse insulinoma cells (MIN6) under different

43 in beta cells to uptake and presentation in islets and peripheral sites.

44 athway components in adult murine pancreatic islets and show that DLL1 and DLL4 are specifically expr

45 gative cells compared with those within both islets and spleen.

46 ve in human T2D islets at population, single islet, and single islet cell levels.

47 e that Gestalt theory may explain why rodent islet architecture has historically been seen as having

48 We found that pancreatic islets are innervated by vagal sensory axons expressing

49 glucose tolerance testing; or in pancreatic islet area or islet morphology, demonstrating that while

50 We further found selective loss of islet-associated beta cells in dogs with sDM and sDMPanc

51 s of molecular clocks operative in human T2D islets at population, single islet, and single islet cel

52 ]) compared with mothers of children without islet autoantibodies (2 [1-4]) (P = .002), but declined

53 tary exposures, heralding the development of islet autoantibodies (IA) and type 1 diabetes (T1D).

54 High-affinity islet autoantibodies predict type 1 diabetes (T1D) but d

55 GN, SETTING, AND PARTICIPANTS: Screening for islet autoantibodies was offered to children aged 1.75 t

56 ) is associated with positivity for multiple islet autoantibodies, irrespective of class II HLA DR-DQ

57 f population-based screening of children for islet autoantibodies.

58 n deficiency-often begins early in life when islet autoantibody appearance signals high risk(1).

59 am of primary care-based screening showed an islet autoantibody prevalence of 0.31%.

60 gh-affinity CD4(+) mimotope (BDC2.5(mim)) of islet autoantigen chromogranin A (ChgA) with or without

61 and proteomic biomarkers for development of islet autoimmunity (IA) and progression to type 1 diabet

62 tion of methylation differences that predate islet autoimmunity and clinical diagnosis may suggest a

63 ion (P < 8.5 x 10(-8)) with progression from islet autoimmunity to type 1 diabetes.

64 ng before and after the onset of preclinical islet autoimmunity.

65 Total pancreatectomy with islet autotransplantation is performed to treat chronic

66 nting large volume of islets necessitated in islet autotransplantation.

67 thymus, spleen, pancreatic lymph nodes, and islets before and after diabetes.

68 nstrate a novel approach aimed at protecting islets before Tx in nonhuman primates (NHPs) (baboons) b

69 tile output from the ganglia can synchronize islet behavior.

70 receptor trafficking can be used to promote islet beta cell survival.

71 1D) retain some functional insulin-producing islet beta cells at the time of diagnosis, the rate of f

72 n as a function of granule age in pancreatic islet beta cells.

73 ity are fundamental properties of pancreatic islet beta cells.

74 Purification of islet beta-, alpha- and delta-cells followed by transcri

75 se chronic lipid exposure is associated with islet beta-cell dysfunction, we investigated LD accumula

76 cores of signaling pathways linked to native islet beta-cell functional maturation, including evidenc

77 The case here is the pancreatic islet beta-cell presented with excessive levels of nutri

78 Consequently, tau knockdown in mouse islet beta-cells facilitates microtubule turnover, causi

79 ed "general" coregulators Sin3a and Sin3b in islet beta-cells, with Sin3a being dispensable for diffe

80 LDs were also found in the islet beta-like cells produced from human embryonic cell

81 t Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan.

82 selenoprotein expression that may influence islet biology and consequentially metabolic disease risk

83 tion is also contributing to dialogue in the islet biology field focused on how to correct the defect

84 iments in the areas of cancer, stem cell and islet biology.

85 Furthermore, knockdown of HB-EGF in rat islets blocks beta-cell proliferation in response to glu

86 Particularly, islet blood flow has been consistently illustrated as on

87 Three models of islet blood flow were previously proposed, all based on

88 se increases lipolysis in non-diabetic human islets, but not in type 2 diabetic (T2D) islets, indicat

89 sis suggests that cholinergic stimulation of islets by pancreatic ganglia resets these endocrine unit

90 stinct islet entrainment of groups of murine islets by pulses of CCh was also observed, providing fur

91 allotransplantation of PVPON/TA-encapsulated islets can elicit localized immunosuppression and potent

92 ing cytosolic calcium overload in pancreatic islets can improve beta-cell survival and function under

93 mutations, including significant pancreatic islet cell adaptation in obesity-associated tumors.

94 Although the molecular pathways underlying islet cell differentiation are beginning to be resolved,

95 that LD enrichment could be impactful to T2D islet cell function.

96 ty type 1 diabetes recipients of intraportal islet cell grafts under antithymocyte globulin induction

97 lets at population, single islet, and single islet cell levels.

98 ar to be significant differences in pancreas islet cell lipid handling between species, and the human

99 insulin sensitivity and restored pancreatic islet cell mass, neuronal innervation and microbiome com

100 in the field require the standardization of islet cell product isolation processes, and this work ai

101 ghted the presence of a heterogeneity in the islet cell product process and product release criteria.

102 a distinguishing transcription factor within islet cell subtype specification.

103 e dually linked in both their secretion from islet cells and their action in the liver.

104 ll-molecule cargoes in beta-cells over other islet cells ex vivo or other cell-types in an organismal

105 uction was assessed by acute knockdown using islet cells from Atf6alpha (flox/flox) mice transduced w

106 TF6 pathways are simultaneously activated in islet cells in response to acute stress and that ATF6alp

107 regulation, and revealed that pig and human islet cells share characteristic features that are not o

108 e performed timed exposures of primary mouse islet cells to ER stressors and measured the early trans

109 system for longitudinal examination of human islet cells undergoing developmental/metabolic/pharmacog

110 ased GLT-induced cytosolic calcium influx in islet cells, and all measured beta-cell-protective effec

111 normal human juvenile pancreatic acinar and islet cells, with numbers subsequently increasing throug

112 primary C57BL/6J mouse and nondiabetic human islet cells.

113 f NF-kappaB transcription factor and control islet cells.

114 ecific NIR FI in the nuclei and cytoplasm of islets cells than in non-treated control mice and this f

115 t that beta-Bmal1 (OV) mice display enhanced islet circadian clock amplitude and augmented in vivo an

116 Moreover, our data suggest that islet clocks orchestrate temporal profiles of insulin an

117 Capsules containing islets co-encapsulated with COOH-coated nanoparticles re

118 anted with even a marginal number of labeled islets compared with controls.

119 The pancreatic islet, consisting of 1-2% mass of the whole pancreas, ha

120 In this study, we show that the human islet contains macrophages in perivascular regions that

121 In conclusion, male and female human islets convert T into DHT and E2 via the intracrine acti

122 f nanobubbles specifically within pancreatic islets, correlating with insulitis.

123 al pouch graft with high tissue volume (1000 islets) could be visualized by positron emission tomogra

124 rocessing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, mi

125 the reductive capacity of nondiabetic human islets cultured at 5 mm glucose for 72 h and exacerbated

126 -199a1 and primiR-199a2 mRNA levels in mouse islets cultured in 10 mm glucose compared with 5.5 mm gl

127 Islet cultures at 20 mm glucose increased apoptosis, whi

128 s, islet microcirculation has no relation to islet cytoarchitecture, which explains its well-known va

129 Islets derived from stem cells hold promise as a therapy

130 Regenerating islet-derived protein 3alpha (REG3alpha) is an antimicro

131 osis factor receptor 1 (TNFR1), regenerating islet-derived protein 3alpha (REG3alpha), and interleuki

132 nknown role of Ogt in exocrine and endocrine islet development.

133 s provide insight into the cellular basis of islet development.

134 gh testing streptozotocin-induced pancreatic islet disruption and fatal diabetes, we found that perip

135 activity of beta-cells within the pancreatic islet drives oscillatory insulin secretion.

136 entify key miRNAs dysregulated in pancreatic islets during T1D progression and to develop a theranost

137 hub cell results in widespread inhibition of islet electrical activity and disruption of their coordi

138 cose metabolism, and diabetes onset, but how islet endocrine cells interact with sensory neurons has

139 ne checkpoint to mitigate IBMIR for enhanced islet engraftment with translational potential.

140 Distinct islet entrainment of groups of murine islets by pulses o

141 Transplanted Bbs4(-/-) islets exhibit delayed re-vascularization and reduced va

142 e db/db mouse islets and ex vivo in C57BL/6J islets exposed to different glucose environments.

143 We show that cultured male and female human islets exposed to T produce DHT and downstream metabolit

144 In these islets, exposure to the 5alpha-R inhibitors finasteride

145 tative real-time PCR of laser microdissected islets for gene expression of proinflammatory cytokines,

146 Alginate-encapsulated islets formed amyloid during culture when functional, an

147 Moreover, glucagon secretion by islets from 31 donors at low glucose (1 mmol/L) was also

148 am molecule of PL signaling, was observed in islets from Adipoq (-/-) dams.

149 lanted with 320 (marginal) or 450 (standard) islets from BALB/c (H-2) mice via the portal vein.

150 on and its expression is highly increased in islets from diabetic mice as well as in plasma of diabet

151 man islet transplantation: (a) poor yield of islets from donated pancreas tissue and (b) the need for

152 in-potentiated insulin secretion response of islets from HFD-fed beta cell-specific Galpha(z)-null mi

153 e is significantly improved as compared with islets from HFD-fed WT controls, which, along with no im

154 shown the relevance of cellular hierarchy in islets from multiple species including human, mouse and

155 rated that the expression of miR-216a in the islets from NOD mice significantly changed during T1D pr

156 y processes are also activated in pancreatic islets from obese animals and humans with obesity and/or

157 Islets from prediabetic obese mice show significantly hi

158 Pancreatic islets from rats encapsulated in the device and implante

159 RGS4 was up-regulated in islets from sparc -/- mice, which correlated with decrea

160 Strikingly, our experiments reveal that islets from T2D patients contain clocks with diminished

161 However, we detected T conversion into E2 in islets from two out of four male donors.

162 slet vascularization is essential for intact islet function and glucose homeostasis.

163 tanding pancreatic genetics, development and islet function could limit progress in developing interv

164 approach for preparing islets that enhances islet function in vitro and reduces immunogenicity.

165 g visceral sensory nerves impacts pancreatic islet function, glucose metabolism, and diabetes onset,

166 Following entrainment, hPSC-derived islets gain persistent chromatin changes and rhythmic in

167 isolated from healthy pregnant women promote islet glucose-stimulated insulin secretion (GSIS) and pe

168 h efficiency to reverse diabetes and sustain islet graft function.

169 Islet graft morphology and vascularization were evaluate

170 Transplantation of SA-PDL1-engineered islet grafts with a short course of rapamycin regimen re

171 d to the long-standing dogma that the rodent islet has a mantle of non-beta-cells and that the islet

172 rance to kidneys, but not thoracic organs or islets, has been achieved in nonhuman primates and human

173 l amplitude as a key regulator of pancreatic islet hormone secretion.

174 earchers to seek functional implications for islet hormone secretion.

175 Pancreatic islet-like distributions of islet hormones were observed in human FFPE tissues prese

176 Detection of amyloid in intraportal islet implants of type 1 diabetes patients has been prop

177 ed the antiapoptotic effect of MN-siCas-3 on islets in culture, resulting in minimal islet loss.

178 Islets in human and rat pancreases were analyzed by immu

179 sed therapeutic target to protect pancreatic islets in the setting of diabetes, little is known about

180 insulin secretion in primary human and mouse islets in vitro and in mice by reducing, at least in par

181 cells (hPSCs) differentiate into pancreatic islets in vitro by profiling DNA methylation, chromatin

182 man islets, but not in type 2 diabetic (T2D) islets, indicating dysregulation of lipolysis in T2D isl

183 In fact, a large proportion of islet-infiltrating B lymphocytes in the NOD mouse model

184 Nerve and islet-infiltrating CD4(+) T cells also differed by expre

185 performed an unbiased examination of diverse islet-infiltrating cells during autoimmune diabetes in t

186 stimulation and increased Treg frequency in islet infiltration and pancreatic lymph nodes.

187 These effects likely act in concert to lower islet inflammation while increasing Tregs in the remaini

188 preserved beta-cell mass and a reduction in islet inflammation.

189 Like human islets, INS1 cells showed visible LDs, glucose responsiv

190 Like human islets, INS1 cells showed visible LDs, glucose-responsiv

191 ted: a finding that correlates with enhanced islet insulin secretion and decreased glucagon secretion

192 Transplantation of 300 syngeneic islets into the peritoneal pouch of recipients reversed

193 has a mantle of non-beta-cells and that the islet is completely separated from the exocrine compartm

194 Pulsatile insulin from pancreatic islets is crucial for glucose homeostasis, but the mecha

195 Human islet isolates with insufficient beta-cell mass for impl

196 Successful islet isolation must address the challenges of severe pa

197 extended distension and trimming time during islet isolation of younger and fibrotic pediatric pancre

198 identify differences in the human pancreatic islet isolation processes within European countries.

199 of this intronic circular RNA in pancreatic islets leads to a decrease in the expression of key comp

200 Pancreatic islet-like distributions of islet hormones were observed

201 The generation of glucose-responsive islet-like organoids that are able to avoid immune detec

202 e proteome landscape of the cells towards an islet-like signature.

203 Islet loss after Tx related to apoptosis, inflammation,

204 3 on islets in culture, resulting in minimal islet loss.

205 text, but the homeostatic functions of human islet macrophages are not known.

206 recipients containing PVPON/TA-encapsulated islets maintained euglycemia and delayed graft rejection

207 nvasive detection of NF-kappaB in pancreatic islets may serve as a potential strategy for monitoring

208 Thus, islet microcirculation has no relation to islet cytoarch

209 y proposed, all based on the assumption that islet microcirculation occurs in an enclosed structure.

210 ve confocal analysis of non-fixed pancreatic islet microscopy we demonstrated that ODND probes may be

211 rast agents can be used to measure increased islet microvasculature permeability and indicate asympto

212 In this Perspective we present key islet miRNA families involved in T2D pathogenesis includ

213 inning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear.

214 st that clustering of endocrine cells during islet morphogenesis is guided, at least in part, by repe

215 out (Robo) receptors are required for proper islet morphogenesis.

216 These advances include studies of islet morphology and human beta-cell gene expression in

217 ance testing; or in pancreatic islet area or islet morphology, demonstrating that while ucOC is impac

218 patic site for transplanting large volume of islets necessitated in islet autotransplantation.

219 ympathetic innervation markers in pancreatic islets of adult dogs with spontaneous DM (sDM), spontane

220 Localization of the tracer in the pancreatic islets of BALB/c nude mice was examined using fluorescen

221 rotein transcript profiles in the pancreatic islets of C57BL/6J mice.

222 99 genes, were differentially methylated in islets of humans with T2D.

223 10 mum pixel size allows us to identify the islets of Langerhans associated with lipid isomer upregu

224 Islets of Langerhans were isolated from genetically iden

225 es (T1D) autoreactive T-cells infiltrate the islets of Langerhans, depleting insulin-secreting beta-c

226 f this circularized intron is reduced in the islets of rodent diabetes models and of type 2 diabetic

227 a), a type I interferon, is expressed in the islets of type 1 diabetic individuals, and its expressio

228 er" cells within the beta-cell network drive islet oscillations and that electrically silencing or op

229 ll populations that display mixed pancreatic islet phenotypes and immaturity.

230 the beta- and alpha-cells of the pancreatic islets play a central role in the regulation of systemic

231 okines and proliferated in response to human islet preparations.

232 orter present in MIN6m9 cells and pancreatic islets, prevented this flush.

233 In INS-1E cells and rat and human islets, proinflammatory cytokines reduced the content of

234 ctive innate repair receptor agonist, exerts islet protective and antiinflammatory properties and imp

235 n and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, an

236 ce of CCK promoting insulin release in human islets remains to be determined.

237 vel strategy to minimize the number of donor islets required from either cadaveric or living donors.

238 Through these mechanisms, islet-resident macrophages underlie the inflammatory res

239 oimmune disease that destroys the pancreatic islets, resulting in insulin deficiency-often begins ear

240 s is to utilize insulin-producing pancreatic islets seeded in a bioscaffold for implantation into dia

241 Intraperitoneal implantation of pancreatic islets seeded within the copolymer bioscaffold supports

242 pient diabetic mice upon implantation of the islet-seeded biomaterial coupled with reduced blood gluc

243 display of SA-PDL1 protein on the surface of islets serves as a practical means of localized immunomo

244 were noted in control mice, in which female islets showed 5 selenoproteins decreased and one increas

245 Quantitative analysis of 665 human islets showed a significant SGLT2 protein colocalization

246 Inhibition of islet-specific autoreactive T cells to rescue beta-cells

247 struction of insulin-producing beta-cells by islet-specific autoreactive T cells.

248 We identified islet-specific CD8+ T cells using high-content, single-c

249 the creation of three-dimensional pancreatic islet structures in both microscale and microfluidic sys

250 mouse islets and 70% of alpha-cells in human islets, suggesting a paracrine alpha to beta-cell signal

251 ficiently displayed on biotin-modified mouse islet surface without a negative impact on their viabili

252 b7a inhibition promotes beta cell growth and islet survival, and protects against activation of apopt

253 , a high frequency (~40%) of HIP2.5-specific islet T cells were identified at both prediabetic and di

254 Notably, in cultured male and female islets, T enhanced glucose-stimulated insulin secretion

255 dy, we describe a new approach for preparing islets that enhances islet function in vitro and reduces

256 ated insulin secretion (NGSIS) in pancreatic islets that is activated by nonesterified free fatty aci

257 e insulin-producing beta-cells in pancreatic islets that is mediated by autoimmune mechanisms.

258 for the propagation of excitation across the islet, there is no obvious electrophysiological mechanis

259 show that exposure of isolated rat and human islets to HB-EGF stimulates beta-cell proliferation.

260 te cross talk both within the islet and from islets to other metabolic tissues, thus providing a uniq

261 he Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-B

262 nd prevents recurrence of autoimmunity after islet transplantation in ~50% of NOD mice.

263 Islet transplantation is a promising treatment for T1D,

264 Islet transplantation is an effective therapy for life-t

265 Islet transplantation is an emerging therapy for type 1

266 ally delay graft destruction in future human islet transplantation studies.

267 Here, we employ a sensitized murine model of islet transplantation to test strategies that promote lo

268 Here we used an allogeneic murine islet transplantation tolerance model to examine the imp

269 ted with type 1 diabetes mellitus (allogenic islet transplantation), or the prevention of surgical di

270 undergoing total pancreatectomy (autologous islet transplantation).

271 h the liver is the primary site for clinical islet transplantation, it poses several restrictions, es

272 ise for overcoming two major issues in human islet transplantation: (a) poor yield of islets from don

273 Islets transplanted into a dissected peritoneal pouch sh

274 in primary human islets and in vivo in human islets transplanted into high-fat diet-fed mice.

275 Human but not mouse islets transplanted into immunodeficient NSG mice effect

276 Human islets transplanted into the peritoneal pouch of diabeti

277 on (IBMIR) causes significant destruction of islets transplanted intraportally.

278 TSPAN-7 is the most abundant islet TSPAN and immunostaining of mouse and human pancre

279 a- and alpha-cells; however, the function of islet TSPAN-7 has not been determined.

280 global translational rate in Lmna (LCS/LCS) islets, two major processes involved in insulin secretio

281 bility and function in primary rat and human islets under GLT.

282 Islet vascularization is essential for intact islet func

283 unclear whether they are also implicated in islet vascularization.

284 cells and induces insulin secretion in human islets via TrkB.T1 identifies a new regulatory function

285 lated mouse islets without a negative impact islet viability and insulin secretion.

286 ve diffusion characteristics can support the islet viability, metabolic activity, and dose necessary

287 Total RNA collected from cultured islets was purified and global miRNA profiling was perfo

288 duction of lizards to replicate experimental islets, we aimed to determine if chemical signal design

289 use insulinoma MIN6 cell line and pancreatic islets, we investigated the effects of G protein subunit

290 Syngeneic C57BL/6 donor islets were transplanted into the peritoneal pouch of di

291 findings using a biochemical approach, human islets were used for pulse-chase experiments.

292 We analyzed beta-cell function in adult islets when SMAD7 was either absent or overexpressed in

293 show that FURIN is highly expressed in human islets, whereas PCs that potentially could provide redun

294 Replacing beta cells using stem cell-derived islets while fostering immune tolerance, exemplified in

295 Culturing islets with atorvastatin (15 uM) for 24 hours decreased

296 robes may be used to distinguish between the islets with high levels of NF-kappaB transcription facto

297 12-h pretreatment of human islets with pertussis-toxin (PTX) improved GSIS and prev

298 Nanotomy allows analyses of complete donor islets with up to macromolecular resolution.

299 splayed on the surface of biotinylated mouse islets without a negative impact islet viability and ins

300 ibrotic pediatric pancreases, gave increased islet yield with improved patient outcomes.