ライフサイエンスコーパス: isoaspartate

1 , indicated that cruciferin subunits contain isoaspartate.

2 fuscimiditide hydrolyses regioselectively to isoaspartate.

3 The link between isoaspartate accumulation and the neurological abnormali

4 ut) mice exhibit greatly increased levels of isoaspartate and typically succumb to fatal epileptic se

5 thod can accurately detect 5 pmol or less of isoaspartate and works with tryptic digests as well as i

6 tein, suggesting that it contains a level of isoaspartate at least 50 times greater than that of the

7 th and without the disease-associated PTM, L-isoaspartate, at position 23 (L-isoAsp23).

8 Subsequently, the existence and site of isoaspartate can be confirmed by electron transfer disso

9 une antigens, of particular interest because isoaspartate can greatly enhance the antigenicity of sel

10 n neurons, accumulated exceptional levels of isoaspartate: collapsin response mediator protein 2 (CRM

11 Surprisingly, this isoaspartate-containing peptide is also a substrate for

12 Moreover, the distribution of isoaspartate-containing proteins in E. coli differed dra

13 particular allows the affinity enrichment of isoaspartate-containing proteins.

14 lived, has been hindered by large amounts of isoaspartate-containing storage proteins.

15 a substantial and consistent increase in the isoaspartate content of tubulin was observed.

16 The enzyme protein L-isoaspartate (D-aspartate) O-methyltransferase (E.C. 2.1

17 mapping of the gene for the murine protein-L-isoaspartate (D-aspartate) O-methyltransferase (EC 2.1.1

18 ave demonstrated that mice lacking protein L-isoaspartate (D-aspartate) O-methyltransferase (Pcmt1-/-

19 Protein-L-isoaspartate (D-aspartate) O-methyltransferases (EC 2.1.

20 Protein L-isoaspartate (D-aspartate) O-methyltransferases (MTs; EC

21 ly conserved cytosolic enzyme, the protein L-isoaspartate(D-aspartate) O-methyltransferase (EC 2.1.1.

22 The widely distributed protein-L-isoaspartate(D-aspartate) O-methyltransferase (PIMT; EC

23 e embryonic stem cells to generate protein L-isoaspartate(D-aspartate) O-methyltransferase-deficient

24 Protein l-isoaspartate-(d-aspartate) O-methyltransferases (EC ), p

25 e post-translational modification sequences: isoaspartate-D7, the phosphorylation of S8, and an N-ter

26 TDP-43 was found to be prone to isoaspartate formation and a substrate for ASRGL1.

27 ay shed important new light on mechanisms of isoaspartate formation in cells and the molecular pathol

28 s suggests that tubulin constantly undergoes isoaspartate formation in vivo, but that the levels are

29 Isoaspartate formation is a ubiquitous post-translation

30 isoaspartate formation occurred in parallel with, but wa

31 The near stoichiometric levels of isoaspartate in S11, estimated at 0.5 mol of isoaspartat

32 rved aspartate residue to a beta-amino acid, isoaspartate, in the lanthipeptide OlvA(BCS(A)).

33 The formation of isoaspartate inserts a methylene group into the protein

34 rtate methyltransferase selectively converts isoaspartates into the corresponding methyl esters.

35 rium between Snn and its hydrolysis products isoaspartate (isoAsp) and aspartate.

36 tion in these MAbs leads to formation of the isoaspartate (IsoAsp) and the cyclic imide (Asu) variant

37 fication of asparagine (Asn) deamidation and isoaspartate (isoAsp) in proteins remains a challenging

38 discovery and characterization of isomerized isoaspartate (isoAsp) residues and D-amino acids within

39 Spontaneous formation of isoaspartates (isoDs) often causes protein damage.

40 Aspartate-to-isoaspartate isomerization in proteins occurs in cells b

41 OGT, enzymes that may catalyze aspartate to isoaspartate isomerization include PARPs, enzymes known

42 soaspartyl peptide, the relationship between isoaspartate levels and S-adenosyl-l-homocysteine produc

43 the possibility that the rat enzyme reduces isoaspartate levels in E. coli proteins, a result predic

44 sensitive HPLC-based method for quantitating isoaspartate levels in peptides and proteins is describe

45 Isoaspartate levels in synapsin from the knock-out mice

46 expressing rat PIMT had significantly lower isoaspartate levels than control cells, especially in st

47 Isoaspartate levels were estimated by the transfer of ra

48 (At3g48330 and At5g50240) encoding protein-l-isoaspartate methyltransferase (EC 2.1.1.77; PIMT), an e

49 The enzyme protein L-isoaspartate methyltransferase (PIMT), present in many b

50 is first specifically methylated by protein isoaspartate methyltransferase (PIMT, EC 2.1.1.77) to th

51 In the initial step, protein isoaspartate methyltransferase selectively converts isoa

52 dation and by isoAsp detection using protein isoaspartate methyltransferase.

53 8), identified within this study, with the l-isoaspartate modification introduced at Asp(58) and Asp(

54 m knock-out mice contains 0.9 +/- 0.3 mol of isoaspartate/mol of synapsin, whereas the levels in wild

55 Protein L-isoaspartate O-methyltransferase (PIMT) is postulated to

56 l-Isoaspartate O-methyltransferase (PIMT) repairs isoD res

57 In almost all organisms, protein L-isoaspartate O-methyltransferase (PIMT, EC2.1.1.77) reco

58 Protein l-isoaspartate O-methyltransferase activity, a repair enzy

59 Isomerization of aspartate to isoaspartate occurs spontaneously in proteins, causes ch

60 ematic and comprehensive characterization of isoaspartate, particularly in complex systems.

61 isoaspartate in S11, estimated at 0.5 mol of isoaspartate per mol of S11, suggests that this unusual

62 Further study of the isoaspartate-prone proteins identified here may help elu

63 These isoaspartate-prone proteins represent a wide range of ce

64 t a chemo-enzymatic detection method for the isoaspartate protein, which in particular allows the aff

65 ethyltransferase in neurons and suggest that isoaspartate-related alterations in the function of pres

66 notype results from the cumulative effect of isoaspartate-related damage to a number of the neuron-ri

67 ic-phase cells, nearly all of the detectable isoaspartate resided in a single 14-kDa protein which we

68 ng four interlocking rings and positions the isoaspartate residue in a solvent exposed loop that is s

69 important role in protein repair, restoring isoaspartate residues formed from asparagine deamidation

70 ersion of asparagine into both aspartate and isoaspartate residues.

71 duce mature proteins capable of converting l-isoaspartate to l-aspartate in small peptide substrates.

72 ts, and animals, catalyzes the conversion of isoaspartate to normal alpha-linked aspartyl bonds and i

73 lecular mass range of 66 to 14 kDa contained isoaspartate, whereas in logarithmic-phase cells, nearly

74 nexpectedly, SsdA contains a beta-amino acid isoaspartate, which is important for enzymatic activity

75 Asparaginase-like-1 protein (ASRGL1) cleaves isoaspartates, which alter protein folding and susceptib

76 In contrast, the introduction of l-isoaspartate within a previously identified anti-chapero

77 uman lens, we localize the accumulation of l-isoaspartate within water-soluble protein extracts prima