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1 issues have discouraged its use in capillary isoelectric focusing.
2 yacrylamide could be excellent for capillary isoelectric focusing.
3 uous separation of subcellular organelles by isoelectric focusing.
4 of them are successful for routine capillary isoelectric focusing.
5 M cone opsins by in situ phosphorylation and isoelectric focusing.
6 monoclonal antibody HC-10, and 2-dimensional isoelectric focusing.
7 ed biochemically by CDK2 kinase assay and by isoelectric focusing.
8 dic shift of r-p53 isoforms separated by gel isoelectric focusing.
9 rms of the enzymes were identified by native isoelectric focusing.
10 nd that 16 (1.2%) demonstrated AmpC bands by isoelectric focusing.
11 at least two different isoforms separable by isoelectric focusing.
12 n intermolecular exchange as shown by native isoelectric focusing.
13 n were then purified by SDS-PAGE followed by isoelectric focusing.
14 from the dye-stained blots and subjected to isoelectric focusing.
15 uring polyacrylamide gel electrophoresis and isoelectric focusing.
16 uring polyacrylamide gel electrophoresis and isoelectric focusing.
17 isolates, 83 (9.2%) produced ESBLs based on isoelectric focusing.
18 ession of class I heavy chains as defined by isoelectric focusing.
19 ting after separation by polyacrylamide-urea isoelectric focusing.
20 filtration, or affinity chromatography or by isoelectric focusing.
21 e concentration required for the traditional isoelectric focusing.
23 sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, amino acid analysis, and mass spec
29 semia (D/D) have a characteristic isoform on isoelectric focusing and 50% reduction in galactose-1-ph
31 ol against prefractionation of peptides with isoelectric focusing and demonstrate that FAIMS fraction
37 an efficient procedure involving preparative isoelectric focusing and ion exchange FPLC chromatograph
38 different backgrounds using a combination of isoelectric focusing and liquid chromatography coupled w
41 and identified using the above method where isoelectric focusing and restriction enzyme analysis fai
43 is paper we have applied sequential solution isoelectric focusing and SDS-PAGE for the proteomic char
45 d demonstrate its feasibility for performing isoelectric focusing and simultaneous fractionation of p
46 mplexes were purified using a combination of isoelectric focusing and sucrose gradient ultracentrifug
47 Embryonic Kidney 293 cells expressing TRPV5, isoelectric focusing and total internal reflection fluor
49 ation was demonstrated using high-resolution isoelectric focusing and two-dimensional gel analysis.
50 chromatography, native gel electrophoresis, isoelectric focusing, and analytical sedimentation equil
51 nked immunosorbent assay for IgG and IgM, by isoelectric focusing, and by inhibition studies to deter
52 eomics, HPLC with electrochemical detection, isoelectric focusing, and extraction of acidic and basic
53 ei CYA was then partially purified by liquid isoelectric focusing, and fractions were subjected to so
55 fferential centrifugation and by preparative isoelectric focusing, and purified to homogeneity on two
56 e extraction, anion exchange chromatography, isoelectric focusing, and reverse-phase high performance
59 eight) isoforms of Mcm1 protein, resolved by isoelectric focusing, are present in vivo; two major pho
61 The two dimensions involve the use of liquid isoelectric focusing as the first phase and nonporous si
62 -linked oligosaccharides from the individual isoelectric focusing bands of a glycoprotein using recom
63 ies could be resolved into discrete forms by isoelectric focusing based apparently on their arabinan
65 in the plasma was estimated by quantitative isoelectric focusing before and after liver transplantat
66 he investigations involved phenotypic tests, isoelectric focusing, beta-lactamase inhibitor studies,
67 and concentrated in a manner that resembles isoelectric focusing but that is applicable to a greater
68 a new technique that is related to capillary isoelectric focusing but uses additional high-voltage po
69 s mixture was fractionated using preparative isoelectric focusing by free-flow electrophoresis, follo
71 tography, and the isozymes were separated by isoelectric focusing, characterized, and quantitated.
72 nzyme by anion-exchange, gel filtration, and isoelectric focusing chromatography yielded a protein mo
73 ion of the powerful combination of capillary isoelectric focusing (CIEF) and Fourier transform ion cy
74 paration techniques, observable by capillary isoelectric focusing (cIEF) and ion exchange chromatogra
75 ion technology in combination with capillary isoelectric focusing (CIEF) and the imaging plug flow (i
81 lex protein mixtures, separated by capillary isoelectric focusing (CIEF) for detection using on-line
83 te feasibility of a combination of capillary isoelectric focusing (CIEF) in a tapered fused silica (F
89 e liquid chromatography (CIEX) and capillary isoelectric focusing (cIEF) methods have been developed
92 k, methods for the high-efficiency capillary isoelectric focusing (CIEF) separation of peptides have
93 a first step toward adaptation of capillary isoelectric focusing (cIEF) to microchannels on a glass
94 of each fraction were separated by capillary isoelectric focusing (cIEF) using a set of amino acids a
95 h involving on-line combination of capillary isoelectric focusing (CIEF) with capillary reversed-phas
96 with tapered geometry suitable for capillary isoelectric focusing (CIEF) with electroosmotic displace
98 strated for on-line combination of capillary isoelectric focusing (CIEF) with electrospray ionization
99 pillary hybrid device to integrate capillary isoelectric focusing (CIEF) with parallel capillary sodi
100 terface for on-line combination of capillary isoelectric focusing (CIEF) with transient capillary iso
101 (boronate-affinity chromatography, capillary isoelectric focusing (CIEF), and standardized DCA2000+ i
102 ion mass spectrometry (ESI-MS) and capillary isoelectric focusing (cIEF), which showed that 0-8 DM1s
108 f compounds is presented, based on capillary isoelectric focusing coupled on-line with electrospray i
110 lyacrylamide-gel electrophoresis and also by isoelectric focusing demonstrate the presence of an acid
111 eight complexes and Blue Native/SDS-PAGE and isoelectric focusing demonstrated that different isoform
112 spinal fluid (CSF) prefractionated by OFFGEL isoelectric focusing drastically increases the number of
114 iquots which were analyzed in parallel by 1D isoelectric focusing electrophoresis (IEF)-laser ablatio
115 The protein was purified to homogeneity by isoelectric focusing electrophoresis (pI 5.8), and it ap
116 d by charge-based analytical methods such as isoelectric focusing electrophoresis and cation or anion
120 cyanine CyDye DIGE Fluor minimal dyes before isoelectric focusing, enabling the labeling of 2-3 sampl
127 nslational modifications and a change in the isoelectric focusing fingerprints relative to the parent
130 c proteomic immunoassay (NIA), consisting of isoelectric focusing followed by sensitive chemiluminesc
133 gradient centrifugation as an alternative to isoelectric focusing for protein separation in the first
135 I, and separated from the native species by isoelectric focusing for use in solid phase binding assa
136 roduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for separat
139 ified using immunoprecipitation, followed by isoelectric focusing gel electrophoresis and specific TR
141 s therefore a good choice to couple directly isoelectric focusing gel electrophoresis with mass spect
146 sis of Triton x-100 insoluble fractions, 2-D isoelectric focusing gels, and confocal microscopy.
150 high peak capacity demonstrates that dynamic isoelectric focusing has great potential as a first dime
151 a proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high
154 evice that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary e
155 A combination of time-resolved microfluidic isoelectric focusing (IEF) and in situ antibody blotting
156 analyzed the proteome of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel e
157 WB) milk/dairy products based on concomitant isoelectric focusing (IEF) detection of B gamma2- and ga
159 e CSL was isolated from whole sporozoites by isoelectric focusing (IEF) following observations that t
161 ase related amyloid beta (Abeta) peptides by isoelectric focusing (IEF) in 75 nL microchannels combin
162 rotocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic c
163 rt of an ongoing investigation of the use of isoelectric focusing (IEF) in microfluidic devices, pH g
164 e of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for c
166 s paradoxical result, we applied a capillary isoelectric focusing (IEF) method to determine the patte
169 ingle strand conformation polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of
170 surface and deep seawater by two-dimensional isoelectric focusing (IEF) OFFGEL-lab-on-chip (LOC) elec
172 t forms of CDGs can be recognized by altered isoelectric focusing (IEF) patterns of serum transferrin
174 lected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital Prote
175 Analysis of C/EBPbeta phosphorylation by isoelectric focusing (IEF) shows that LAP is present in
176 nes bla(TEM), bla(SHV), and bla(OXA), and in isoelectric focusing (IEF) tests, all isolates demonstra
177 l (2D) chromatography platform that includes isoelectric focusing (IEF) through immobilized pH gradie
178 L analytical glass syringe has been used for isoelectric focusing (IEF) utilizing the stainless-steel
181 tion/separation system, combining non-native isoelectric focusing (IEF) with sodium dodecyl sulfate (
185 cs, use of peptide markers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptame
191 pproaches [native, urea, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE
196 noprobed isoelectric focusing is composed of isoelectric focusing in a large pore-size polyacrylamide
198 ecting protein phosphorylation that involves isoelectric focusing in a vertical mini-gel format follo
201 r reports the application of ampholyte-based isoelectric focusing in poly(dimethylsiloxane) (PDMS) us
203 dimensional gel electrophoresis studies with isoelectric focusing in the first dimension identified n
206 e high resolution of immobilized pH gradient isoelectric focusing (IPG-IEF) for the analysis of these
210 , and with a spatial resolution matching the isoelectric focusing, it is therefore a good choice to c
211 ry positioning also allows on-line capillary isoelectric focusing mass spectrometry with in-probe foc
212 present an automated nondenaturing capillary isoelectric focusing-mass spectrometry (ncIEF-MS) method
214 inator device to our multijunction capillary isoelectric focusing (MJ-CIEF) instrument and perform di
216 ulting in glass-based microfluidic free-flow isoelectric focusing (muFFIEF) chips with integrated pH
217 ques, such as free-flow isotachophoresis and isoelectric focusing, no complex buffer compositions are
220 n emerging separation method for preparative isoelectric focusing of complex peptide mixtures, as a t
224 rate the use of VICAT reagents together with isoelectric focusing of peptides on an immobilized gel s
225 imensional peptide separation methods (here, isoelectric focusing of peptides) are incorporated into
226 all amounts of nucleic acids; more recently, isoelectric focusing of proteins also has been adapted t
233 introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionator to fraction
236 lysis detected a HLA-A H chain with a unique isoelectric-focusing pattern, which does not associate w
238 proteins and by sodium dodecyl sulfate- and isoelectric focusing polyacrylamide gel electrophoresis,
240 d anion exchange chromatography, preparative isoelectric focusing, polymer-based C18-like, and analyt
241 cence difference labeling followed by liquid isoelectric focusing prefractionation/two-dimensional ge
243 ese charged carbohydrates in determining the isoelectric focusing profile has been limited to observi
244 ples from all the patients showed a modified isoelectric-focusing profile, identical to hepatic EPO t
246 ophagus cell line and separated by capillary isoelectric focusing, reproducibly generating dozens of
250 imately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins wi
252 lian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immuno
253 itro, the modified proteins were analyzed by isoelectric focusing, SDS polyacrylamide electrophoresis
254 analysis, immunoprecipitation, 2-dimensional isoelectric focusing/SDS PAGE and immunoblot analysis sh
260 absolute quantification approach with OFFGEL isoelectric focusing separation and liquid chromatograph
263 henotype of adult HPFH transgenic animals by isoelectric focusing showed the presence of hybrid mouse
264 system, we could visualize a high resolution isoelectric focusing signature of PKCdelta upon stimulat
265 s documented by mass spectrometry, SDS-PAGE, isoelectric focusing, size-exclusion chromatography with
266 modifications of BSA were investigated by 2D isoelectric focusing sodium dodecyl sulfate polyacrylami
267 , as judged by separation on two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylami
270 diac myofibrils separated by two-dimensional isoelectric focusing subjected to a Western blot with a
272 egrated and rapid multispectral immunoprobed isoelectric focusing technique for identifying specific
274 utation prompted us to develop an analytical isoelectric focusing technique that resolves endogenous
275 a rapid and efficient procedure utilizing an isoelectric focusing technique to obtain vaccines from t
278 mass spectrometry, the expected mobility on isoelectric focusing, the calculated amino acid composit
280 uestion, we used affinity chromatography and isoelectric focusing to fractionate plasma immunoglobuli
281 ese DNA-assisted RCA techniques in capillary isoelectric focusing to resolve endogenous signaling tra
283 zyme forms were separated from each other by isoelectric focusing to show that heterotetramers were f
284 , and can be used, together with transferrin isoelectric focusing, to diagnose patients with CDG-Ia a
287 amide gel electrophoresis (PAGE) and agarose isoelectric focusing under nondenaturing conditions.
288 mass without resorting to ampholytes and/or isoelectric focusing, using a single- and two-step free-
294 spectrometry (and, for comparative purposes, isoelectric focusing) were used to characterize venoms f
297 sisting of a single 3.75 cm long channel for isoelectric focusing, which is sampled in parallel by 20
299 se blood were determined after separation by isoelectric focusing with an octyl-bonded capillary.