ライフサイエンスコーパス: isopeptide

1 is not rate-limiting for hydrolysis of this isopeptide.

2 breaking of Nepsilon-(gamma-glutamyl)lysine isopeptides.

3 es and their subsequent functionalization to isopeptides.

4 g that the proteasome can produce sufficient isopeptide Ag to evoke a T cell response.

5 Finally, we show that isopeptide Ags are immunogenic in vivo.

6 be generally applicable to the synthesis of isopeptide analogues in which an oxirane replaces the sc

7 rked and dose-dependent inhibitory effect on isopeptide and ester bond formation.

8 We conclude that both isopeptide and ester bond ubiquitination regulate protea

9 w that ubiquitin is attached to hD4R through isopeptide and ester bonds.

10 ly catalyses the cleavage of ubiquitin-like (isopeptide and linear) bonds from target proteins.

11 table features include the first examples of isopeptide and thioamide backbone substitutions in ribos

12 in with the capacity to hydrolyze thioester, isopeptide, and peptide bonds.

13 njugates generated in H. volcanii as well as isopeptide- and linear-linked SAMP1-MoaE in purified for

14 nists of endothelin-1 (ET-1) and its related isopeptides are important tools defining the role of ET

15 Additionally, we show that isopeptides are more stable toward further proteasomal p

16 that catalyze the hydrolysis of peptides and isopeptides are often susceptible to inactivation by ele

17 talytically competent and compatible with di-isopeptide binding, the Ins-1 segment obstructs access t

18 ution is associated with a unique interchain isopeptide bond (glutamic acid-lysine), which flanks con

19 resolving the thioester intermediate into an isopeptide bond (transamidation) but not in thioester hy

20 Ub) carboxylate group either by a peptide or isopeptide bond abolishes BUZ-domain interaction.

21 in-like-modifier conjugates bearing a native isopeptide bond and only one point mutation in the linke

22 of cGAS to a lysine residue, cleavage of the isopeptide bond and poly-cGASylation.

23 produce PCNA-Ub at high yield with a native isopeptide bond and study its Usp1/UAF1-dependent deconj

24 A mutant lacking the CNA(1) isopeptide bond assembled deformed pilin subunits that f

25 This singular isopeptide bond at the asymmetric egress site confers an

26 threaded knot structure that is formed by an isopeptide bond attaching the N-terminus of the peptide

27 structure in these peptides derives from an isopeptide bond attaching the N-terminus of the peptide

28 yme forms an epsilon-(gamma-glutamyl) lysine isopeptide bond between a lysine donor from one protein

29 specificity and catalyze the formation of an isopeptide bond between a lysine residue of the substrat

30 on signal, and catalyzes the formation of an isopeptide bond between a substrate (or ubiquitin) lysin

31 ortase-catalyzed introduction of a K190-T494 isopeptide bond between adjacent SpaA pilins causes them

32 ation activity that leads to formation of an isopeptide bond between glutamine and lysine residues fo

33 XIII (FXIIIa) catalyzes the formation of an isopeptide bond between glutamine at position two in alp

34 in FbaB contains a domain with a spontaneous isopeptide bond between Lys and Asp.

35 desulfurization to yield an entirely native isopeptide bond between substrate proteins and ubiquitin

36 polyubiquitin chain, which is defined by an isopeptide bond between the C terminus of one ubiquitin

37 in, monomers are linked to each other via an isopeptide bond between the C-terminal glycine of one Ub

38 pically thought to occur via formation of an isopeptide bond between the C-terminal glycine residue o

39 ovalently attached to a substrate through an isopeptide bond between the ubiquitin carboxy terminus a

40 karyotic cells, involves the formation of an isopeptide bond between ubiquitin (Ub) and targeted prot

41 e bond as well as a HIP-isomer containing an isopeptide bond by linkage to the carboxylic acid side c

42 G15 substrates, as well as in their rates of isopeptide bond cleavage of K48- and K63-linked polyubiq

43 The recent discovery of intramolecular isopeptide bond cross-links, formed autocatalytically, i

44 e active site, which exposes and orients the isopeptide bond for hydrolysis.

45 proposed to contain activities in catalyzing isopeptide bond formation (ubiquitin ligation) and subst

46 linkage of SpyTag shed light on spontaneous isopeptide bond formation and should provide a targetabl

47 In theory, polyubiquitination can occur by isopeptide bond formation at any of the seven lysine res

48 r proteins through a mechanism that involves isopeptide bond formation between Gln and Lys residues a

49 r proteins through a mechanism that involves isopeptide bond formation between Gln and Lys residues.

50 on-lysine transferase EC 2.3.2.13) catalyzes isopeptide bond formation between glutamine and lysine r

51 enzymatic protein cross-linking activity via isopeptide bond formation between glutamine and lysine r

52 ein kinase fold that catalyzes ATP-dependent isopeptide bond formation between the amino group of fre

53 Streptococcus pneumoniae adhesin and enables isopeptide bond formation between two peptide tags: DogT

54 antibody labeling approach which is based on isopeptide bond formation between two recognition peptid

55 mportant for the subsequent reaction step of isopeptide bond formation between two ubiquitin molecule

56 ude split intein reconstitution, spontaneous isopeptide bond formation by SpyTag and SpyCatcher and s

57 Spontaneous isopeptide bond formation, a stabilizing posttranslation

58 or catalysis of E2- and E2/RING E3-dependent isopeptide bond formation, but dispensable for upstream

59 via a cysteine protease and cyclization via isopeptide bond formation.

60 asparagine are dispensable for catalysis of isopeptide bond formation.

61 hat utilize distinct mechanisms in promoting isopeptide bond formation.

62 sate, based on SpyTag-SpyCatcher coupling by isopeptide bond formation.

63 , that locks itself together via spontaneous isopeptide bond formation.

64 ilin monomer, which joins two pilins with an isopeptide bond formed between threonine and lysine.

65 the modification by specific cleavage of the isopeptide bond formed with Pup.

66 inked by way of the transglutaminase-induced isopeptide bond has not been reported.

67 ubunit is found approximately 2.4 A from the isopeptide bond in the partly hydrophobic pocket that co

68 TAM directed AMSH cleavage toward the distal isopeptide bond in tri-ubiquitin chains.

69 upport that (1) the cleavage of the proximal isopeptide bond is not a prerequisite for proteasomal de

70 onstrate that the Nepsilon-Gly-L-homothiaLys isopeptide bond is processed to a similar extent by deub

71 our finding that BRISC could not cleave the isopeptide bond joining a ubiquitin to a non-ubiquitin s

72 types of modifications, like succinimide or isopeptide bond moieties due to cross-linking between As

73 s an enzyme that specifically hydrolyzes the isopeptide bond of lasso peptides, rendering these pepti

74 demonstrated by isolating and sequencing the isopeptide bond of polyubiquitin.

75 ss, we systematically examined the effect of isopeptide bond position and molecular sizes of auxiliar

76 ovel fluorescent polarization assay using an isopeptide bond substrate mimetic (IsoMim) that can be m

77 FETCH leverages a spontaneous covalent isopeptide bond that forms between the 23-amino acid Dog

78 abilized by factor XIIIa through an amide or isopeptide bond that ligates adjacent fibrin monomers.

79 yChIP takes advantage of a specific covalent isopeptide bond that rapidly forms between the 15-amino

80 -Thr-Glu-Arg cross-linked via an Lys3-Gln102 isopeptide bond to Ala-Val-Pro-Ser-Gln102-Lys.

81 pathways that attach the polypeptides via an isopeptide bond to epsilon-lysyl amino group(s) in the t

82 s a peptide engineered to form a spontaneous isopeptide bond to its protein partner SpyCatcher.

83 n specificity and the mechanism to cleave an isopeptide bond to release m-DAP.

84 NF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin.

85 r an enzyme that specifically hydrolyzes the isopeptide bond to yield the linear peptide.

86 g an aspartyl catalyst, did not generate the isopeptide bond within the jelly-roll structure of XNA.

87 ntermolecular epsilon-(gamma-glutamyl)lysine isopeptide bond(s) and the formation of high molecular m

88 erminal tails (branch initiation, through an isopeptide bond), and additional glutamates can extend t

89 rtners which lock together via a spontaneous isopeptide bond).

90 Besides the class-defining isopeptide bond, other post-translational modifications

91 ns, each of which harbours an intramolecular isopeptide bond, previously described in several Gram-po

92 DUBs) as that of a native Nepsilon-Gly-L-Lys isopeptide bond, thereby establishing the utility of TEC

93 hrough mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops present in I

94 thod to engineer functional control into the isopeptide bond-forming SpyTag/SpyCatcher protein ligati

95 tide bond-linked polyubiquitin, but not with isopeptide bond-linked polyubiquitin, indicating that th

96 sine side chain of a substrate protein by an isopeptide bond.

97 uorine substitution adjacent to the scissile isopeptide bond.

98 an electrophilic replacement of the scissile isopeptide bond.

99 ine side chains of the target protein via an isopeptide bond.

100 proteases that specifically cleave only the isopeptide bond.

101 he Ub that donates the C-terminal Gly to the isopeptide bond.

102 n site" binds the Ub that donates Lys to the isopeptide bond.

103 lariat knot-like fold and the presence of an isopeptide bond.

104 stabilizing latch that covers the K190-T494 isopeptide bond.

105 most cases, a lysine of the substrate via an isopeptide bond.

106 developmentally downregulated gene 8 (Nedd8)isopeptide bond.

107 so remodel ubiquitin chains to hydrolyse the isopeptide bond.

108 strands are linked through an intramolecular isopeptide bond.

109 cceptor lysine, resulting in formation of an isopeptide bond.

110 plug-and-play platform using the spontaneous isopeptide-bond formation of the SpyTag:SpyCatcher syste

111 significantly only to generic protein terms (isopeptide-bond/ubiquitin-like conjugation) and pulse-ch

112 sed in wheat-based foods through introducing isopeptide bonds (e-y glutamyl-lysine).

113 Isopeptide bonds (IPBs)-formed between the amine group o

114 ses (MTGs) catalyze the formation of Gln-Lys isopeptide bonds and are widely used for the cross-linki

115 mate or aspartate side chains to form stable isopeptide bonds and be used for the precise labeling of

116 The isopeptide bonds and disulfide bonds played important ro

117 ical studies demonstrate that although these isopeptide bonds are dispensable for fimbrial assembly,

118 These covalent isopeptide bonds are of great physiological significance

119 In a mixed chain, not all the isopeptide bonds are restricted to a specific lysine of

120 by incorporating site-specific interhelical isopeptide bonds as the redox-sensitive disulfide surrog

121 otein substrate occurred exclusively through isopeptide bonds at a lysine epsilon-amino group within

122 n which the 8.6-kDa polypeptide is linked by isopeptide bonds between carboxyl termini and Lys-48 res

123 Poly-Ub chains, linked through isopeptide bonds between internal Lys residues and the C

124 be ligated together through the formation of isopeptide bonds between Lys48 and Gly76 of successive u

125 Factor XIII catalyzes the formation of isopeptide bonds between noncovalently associated fibrin

126 s an autocatalytic reaction that creates 420 isopeptide bonds between proteins.

127 formation of epsilon-(gamma-glutamyl)lysine isopeptide bonds between specific Gln and Lys residues.

128 collision-induced dissociation demonstrated isopeptide bonds between the C-terminal glycine of SAMP2

129 moiety or a chain of Ub molecules joined by isopeptide bonds between the C-terminus of one Ub with o

130 for fiber formation as they create covalent isopeptide bonds between the sortase recognition motif a

131 et share a common beta-grasp fold, also form isopeptide bonds by a mechanism that appears streamlined

132 Removal of the isopeptide bonds by mutagenesis readily allowed Spy0128

133 e covalently crosslinked together via lysine-isopeptide bonds by pilus-specific sortase enzymes.

134 s) are defined as enzymes capable of forming isopeptide bonds by transfer of an amine onto glutaminyl

135 ture of these reduction-insensitive bonds is isopeptide bonds formed between side chains of lysine an

136 of cross-linking of constituent proteins by isopeptide bonds formed by transglutaminases.

137 ach acquire intramolecular lysine-asparagine isopeptide bonds formed via catalytic glutamic acid or a

138 at mutants unable to form the intramolecular isopeptide bonds in the CNA(2) or CNA(3) domains retain

139 or studies have demonstrated the presence of isopeptide bonds in the sheath and cuticle of filarial p

140 HK97 subunits cross-link via isopeptide bonds into oligomers that are closed rings of

141 Cyclization via domains forming spontaneous isopeptide bonds is a general strategy to generate resil

142 dification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines

143 re a family of >100 proteases that hydrolyze isopeptide bonds linking ubiquitin to protein substrates

144 Isopeptidase T (IPaseT) can hydrolyze isopeptide bonds of polyubiquitin (polyUb) chains, simpl

145 Prokaryotes form ubiquitin (Ub)-like isopeptide bonds on the lysine residues of proteins by a

146 The chemically identical isopeptide bonds recently documented in bacterial pili h

147 rom the strategically located intramolecular isopeptide bonds recently identified in the x-ray struct

148 tin (Ub) chains linked through Lys-48-Gly-76 isopeptide bonds represent the principal signal by which

149 olypeptides covalently linked via peptide or isopeptide bonds to the C-terminal glycine of ubiquitin.

150 nds its protein partner SpyCatcher and forms isopeptide bonds under physiological conditions.

151 n chains linked internally via epsilon-amino isopeptide bonds using Lys48 and can process some, but n

152 cy-thiolactone underlies its ability to form isopeptide bonds with protein lysine residues (N-Hcy-pro

153 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

154 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

155 Hcy-thiolactone has the ability to form isopeptide bonds with protein lysine residues, which gen

156 alkoxyamines, resulting in the formation of isopeptide bonds with varied susceptibilities to hydroly

157 MALDI-TOF revealed the formation of covalent isopeptide bonds, and spectroscopic and FTIR analyses de

158 oil proteins, in contrast to those linked by isopeptide bonds, are dispersed by treatment with chemic

159 olyubiquitin chain assembled through K48-G76 isopeptide bonds, rather than by ligation of monoubiquit

160 of substrates was thought to occur only via isopeptide bonds, typically to lysine residues.

161 ll as other protein pairs that form covalent isopeptide bonds, will facilitate many additional in viv

162 including native ISG15 conjugates linked via isopeptide bonds.

163 was active in vivo based on the detection of isopeptide bonds.

164 establishing epsilon-(gamma-glutamyl)lysine isopeptide bonds.

165 in chains linked exclusively through K48-G76 isopeptide bonds.

166 attached to the B-carboxylate sidechains by isopeptide bonds.

167 inines attached to their side chains through isopeptide bonds.

168 omain molecule stabilized by two intradomain isopeptide bonds.

169 tin molecules are connected through specific isopeptide bonds.

170 We recently discovered that crosslinking isopeptide bridges within the de novo helical trimers ad

171 one of two minor components of the isolated isopeptides, but neither the major isopeptide nor the ot

172 are normal peptides, and we demonstrate that isopeptides can bind to HLA-A2.1 and HLA-A3 with high af

173 ly on the catalytic cysteine, abrogating the isopeptide-cleaving activity without affecting these enz

174 ns, it efficiently deubiquitinates a Ub-SUMO isopeptide conjugate and a Ub-SUMO fusion protein.

175 ty-based probes and stress the importance of isopeptide-containing reagents for validating isopeptida

176 perties of the derivative bearing an A11-B10 isopeptide cross-link suggests that the disulfide has a

177 Herein, we introduce a covalent interhelical isopeptide cross-linking strategy that significantly enh

178 Microscopic localization and isopeptide cross-linking studies suggest that TIG3 and t

179 mmunohistochemical analysis, indicating that isopeptide cross-links are important for the stabilizati

180 This enzyme catalyzes the formation of isopeptide cross-links between fibrin molecules in nasce

181 Isopeptide cross-links form between asparagine and lysin

182 Furthermore, isopeptide cross-links were reduced in both uninjured sk

183 acellular matrix proteins by introduction of isopeptide cross-links.

184 lutamyl-epsilon-Lys (Gln40(Ub)-Lys92(Ube2N)) isopeptide crosslink using a transglutaminase mechanism.

185 (TGases) are enzymes that catalyze covalent isopeptide crosslinks between reactive lysine and glutam

186 om human epidermal cell envelopes containing isopeptide crosslinks inserted by transglutaminases in v

187 Because the U-II isopeptide family is highly conserved across species, bo

188 that recognizes diglycine (diGly)-containing isopeptides following trypsin digestion.

189 at specifically eliminates RING E3-catalyzed isopeptide formation but not HECT E3 transthiolation (N7

190 wo epoxide-containing peptidomimetics of the isopeptide, glutamyl-gamma-glutamate, have been synthesi

191 yme-product complex is rate-limiting in this isopeptide hydrolysis reaction.

192 nation and elicits greater ubiquitin-protein isopeptide ligase (E3) activity toward p53, thereby incr

193 owever, the mRNA levels of ubiquitin-protein isopeptide ligase (E3) carboxyl terminus of Hsp70-intera

194 the identification of the ubiquitin-protein isopeptide ligase (E3) EFP (estrogen-responsive finger p

195 We previously described a ubiquitin-protein isopeptide ligase (E3) from erythroid cells that assembl

196 in) and RING domains) is a ubiquitin-protein isopeptide ligase (E3) that is important for the control

197 The ubiquitin-protein isopeptide ligase (E3) that ubiquitinated IRF-8 was like

198 with Hsp40, Hsp70, and the ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase carboxyl terminu

199 The SCF-ROC1 ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase complex targets

200 ergy in lymphocytes) is an ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase necessary for th

201 or for the SCFHOS/betaTrCP ubiquitin-protein isopeptide ligase (E3) via one canonical and one cryptic

202 Mutations of parkin, a protein-ubiquitin isopeptide ligase (E3), appear to be the most frequent c

203 unctions like an anti-SUMO ubiquitin-protein isopeptide ligase (E3), interacting both with HSF2 and t

204 CF(Skp2/Cks1) complex, the ubiquitin-protein isopeptide ligase (E3).

205 by the dipeptide Leu-Ala, an inhibitor of Ub isopeptide ligase (E3).

206 Parkin is a ubiquitin-protein isopeptide ligase able to translocate to the mitochondri

207 dTopors protein possesses ubiquitin-protein isopeptide ligase activity in vitro and that dTopors med

208 strate that San1 possesses ubiquitin-protein isopeptide ligase activity in vitro, and the ubiquitin-p

209 p90 substrates through the ubiquitin-protein isopeptide ligase activity of CHIP.

210 activity in vitro, and the ubiquitin-protein isopeptide ligase activity of San1 is required for its f

211 cting proteins through the ubiquitin-protein isopeptide ligase activity of their RING domain.

212 an IAPs by promoting their ubiquitin-protein isopeptide ligase activity.

213 ine phosphorylation of the ubiquitin-protein isopeptide ligase c-Cbl and promoted its interaction wit

214 function while binding the ubiquitin-protein isopeptide ligase Cbl.

215 the ElonginB/C-Cullin2-CIS ubiquitin-protein isopeptide ligase complex.

216 Ste6p* is dependent on the ubiquitin-protein isopeptide ligase Doa10p and is largely independent of t

217 The ubiquitin-protein isopeptide ligase E6-associated protein (E6AP) associate

218 Sina protein, function as ubiquitin-protein isopeptide ligase enzymes to target a wide range of cell

219 The Ras effector and ubiquitin-protein isopeptide ligase family member IMP acts as a steady-sta

220 e on MDM2 and inhibits its ubiquitin-protein isopeptide ligase function, resulting in the stabilizati

221 largely independent of the ubiquitin-protein isopeptide ligase Hrd1p/Der3p, whereas the opposite is t

222 gene, pVHL, functions as a ubiquitin-protein isopeptide ligase in regulating HIF-1 protein turnover,

223 PIASxbeta/Siz2, which is a ubiquitin-protein isopeptide ligase in the SUMO pathway, as the potential

224 pression of beta-TrCP1, an ubiquitin-protein isopeptide ligase interacting with ATF4 by RNA interfere

225 The ubiquitin-protein isopeptide ligase RNF8 was critical for 53BP1 focus targ

226 eport that the RING finger ubiquitin-protein isopeptide ligase Siah2 binds to and targets OGDHC-E2 fo

227 , a chaperone-dependent E3 ubiquitin-protein isopeptide ligase that is known to ubiquitylate other hs

228 in homologue Nrdp1/FLRF, a ubiquitin-protein isopeptide ligase that ubiquitinates ErbB3 and ErbB4, wa

229 Ps also possess E3 ligase (ubiquitin-protein isopeptide ligase) activities implicated in both caspase

230 ive form of the E3 ligase (ubiquitin-protein isopeptide ligase) that targets Runx2 for degradation (S

231 PMEPA1 protein is a NEDD4 (ubiquitin-protein isopeptide ligase)-binding protein, which negatively reg

232 As a ubiquitin-protein isopeptide ligase, cIAP1 ubiquitinated caspase-3 and -7,

233 hat inactivation of Cbl, a ubiquitin-protein isopeptide ligase, partially rescues T cell development

234 Nedd4-2, a ubiquitin-protein isopeptide ligase, tags ENaC with ubiquitin for internal

235 l ubiquitin exerts its effect at the step of isopeptide ligase-catalyzed (E3) ubiquitin conjugation s

236 culocyte extract demonstrated RAD6 supported isopeptide ligase-dependent degradation only through Lys

237 (PIAS) family members are ubiquitin-protein isopeptide ligase-small ubiquitin-like modifier ligases

238 n of Trim32, a RING domain ubiquitin-protein isopeptide ligase-ubiquitin ligase mutated in human limb

239 to both A3G and a Cullin 5 ubiquitin-protein isopeptide ligase.

240 Parkin is a ubiquitin-protein isopeptide ligase.

241 aining protein (beta-TrCP) ubiquitin-protein isopeptide ligase.

242 s on their catalytic context with respect to isopeptide ligase.

243 ts sumoylation, thus serving as SUMO-protein isopeptide ligases (E3) for SnoN sumoylation.

244 Siah proteins are ubiquitin-protein isopeptide ligases (E3) that have been implicated in a v

245 in, a motif often found in ubiquitin-protein isopeptide ligases (E3).

246 , which are believed to be ubiquitin-protein isopeptide ligases (type E3).

247 hat cIAP1 and cIAP2 are E3 ubiquitin-protein isopeptide ligases (ubiquitin ligases) for Smac.

248 tors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathway

249 known as an adaptor in SCF ubiquitin-protein isopeptide ligases.

250 d asparaginyl ligase OaAEP1 catalyzes direct isopeptide ligation by accepting an internal 2,3-diamino

251 We provide the first demonstration that isopeptide ligation, a noncanonical activity of the enzy

252 s represent the first examples of successful isopeptide ligations starting from O-acyl Tyr-peptides.

253 ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an

254 x of these proteins stabilized by a covalent isopeptide linkage between Glu 112 and Lys beta400 of th

255 To determine the isopeptide linkage formed by BRCA1/BARD1-dependent polyu

256 but is instead dictated by how the substrate isopeptide linkage is oriented within the enzyme active

257 The isopeptide linkage of a polyubiquitin chain is a particu

258 Among seven possible isopeptide linkage sites in ubiquitin, K48 and K63 occur

259 ically conjugates to target proteins through isopeptide linkage to the epsilon-amino group of lysine

260 s by human UCH-L3 of a 13-residue peptide in isopeptide linkage with ubiquitin, consistent with consi

261 ubiquitin chains containing a single type of isopeptide linkage, and that chains composed of linkages

262 to the SpyCatcher protein via a spontaneous isopeptide linkage, thereby offering a genetically encod

263 ss spectrometry (ESI-MS) method to determine isopeptide linkage-selectivity and affinity of poly-Ub.U

264 t whose Lys-48 side chain is involved in the isopeptide linkage.

265 at 3.2 A resolution confirms the site of the isopeptide linkage.

266 somal degradation by formation of a covalent isopeptide linkage.

267 g that potentially not only peptide but also isopeptide linkages could be formed.

268 allow hOtu1 to discriminate among different isopeptide linkages in polyubiquitin substrates.

269 SpyCatcher, that spontaneously form covalent isopeptide linkages under physiological conditions.

270 directly to lysine residues on Ubc9, forming isopeptide linkages.

271 orks without affecting synthesis of standard isopeptide linkages.

272 -coil interactions or through intermolecular isopeptide linkages.

273 x, which cleaves K11, K48, and K63 ubiquitin isopeptide linkages.

274 bstrates that link ubiquitin via peptide and isopeptide linkages.

275 ic ubiquitin-like protein) is deamidated and isopeptide linked to proteins by a mechanism distinct fr

276 uitins are added onto a substrate to form an isopeptide-linked 'polyubiquitin' chain, which targets s

277 ognizes K63-linked polyUb, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyU

278 portantly, these antibodies do not recognize isopeptide-linked diglycine (ubiquitin) modifications on

279 ylases, harboring catalytic activity against isopeptide-linked Fubi.

280 -specifically modify target proteins with an isopeptide-linked glycylglycine moiety that serves as an

281 ecombinant USP16 cleaved pro-ISG15 and ISG15 isopeptide-linked model substrates in vitro, as well as

282 further expands the range and complexity of isopeptide-linked products that can be accessed with OaA

283 nabling straightforward formation of diverse isopeptide-linked products under simple reaction conditi

284 ility of the 20S proteasome to proteolyze an isopeptide-linked ubiquitin-conjugate.

285 tein fusion; UBICEP52) and representative of isopeptide-linked ubiquitin-protein conjugates [ubiquiti

286 Glutamylation-the addition of branched (isopeptide-linked) glutamate chains-is the most evolutio

287 ethods described here for the preparation of isopeptide-linked, fully folded substrates will aid in t

288 BapA1 possesses nine putative pilin isopeptide linker domains which are crucial for pilus as

289 SP11 in interacting with mono-ubiquitin, the isopeptide mimetic ubiquitin-GGG, and the C-terminal tru

290 DUB-resistant Ub probes is reported based on isopeptide-N-ethylated dimeric or polymeric Ub chains, w

291 ree quantitative (LFQ) MS indicated that the isopeptide-N-ethylated Ub probes may complement the tria

292 We show how site-specific isopeptide (Nepsilon-Gly-L-homothiaLys) bonds are forged

293 isolated isopeptides, but neither the major isopeptide nor the other minor isoform was found within

294 ases, enzymes that catalyze the formation of isopeptide protein-protein cross-links, are key enzymes

295 We confirmed that this isopeptide replacement is resistant to DUBs and to shavi

296 Exposure of these cells to NmU isopeptides resulted in an increase in intracellular Ca(

297 led equipotent, high affinity binding of NmU isopeptides to membranes prepared from D10.G4.1 cells.

298 echniques, hydrolysis of the non-fluorinated isopeptide was characterized by a burst phase followed b

299 The beta-lysyl-lysine isopeptide was identified in the exhaustive Pronase dige

300 eptides represented over 95% of the isolated isopeptides, which, at 2.5 nm concentration, induced the