ライフサイエンスコーパス: isotope labeling

1 pids in monolayers and bilayers using stable isotope labeling.

2 icus rubellus were investigated using stable isotope labeling.

3 3-cyanobenzofurans with site specific (13)C-isotope labeling.

4 one-dimensional (1)H NMR spectra without any isotope labeling.

5 ned with experiments that incorporate stable isotope labeling.

6 liminating the need for either heteroatom or isotope labeling.

7 on or near the surface, without the need for isotope labeling.

8 quantification of the pattern and extent of isotope labeling.

9 sion electron microscopies as well as stable isotope labeling.

10 ion mass spectrometry (NanoSIMS) and stable isotope labeling.

11 of the aforementioned structures with (13)C-isotope labeling.

12 chlorophyll and proteins, a combined stable isotope labeling (15N)/mass spectrometry method was used

13 Through strategic isotope labeling, all nonhydrogen atoms were distinct fr

14 ction of metabolites of interest with stable isotopes labeling allowed the discovery of new metabolit

15 fatty acids and branched-chain amino acids), isotope labeling analyses supported the transformation o

16 icrobial mineralization using reverse stable isotope labeling analysis (RIL) of dissolved inorganic c

17 T, which was further confirmed by the stable isotope labeling analysis using deuterated acetate.

18 alysis of cardiomyocytes by combining stable isotope labeling and click chemistry with subsequent mas

19 te whole-plant metabolic profiling by stable isotope labeling and combustion isotope-ratio mass spect

20 e one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation sig

21 ron diffraction in combination with hydrogen isotope labeling and empirical potential structure refin

22 Mechanistic studies including isotope labeling and Hammett correlation suggest that de

23 By combining selective isotope labeling and high-resolution solid-state Li NMR,

24 Here we used stable isotope labeling and isotopomer analysis to trace sterol

25 tion are typically conducted by using stable isotope labeling and label-free quantitation approaches.

26 of Met sulfoxide in proteins accurately, an isotope labeling and LC-MS peptide mapping method was de

27 ormational changes and dynamics using stable-isotope labeling and mass spectrometry (CDSiL-MS), which

28 Using metabolic stable isotope labeling and mass spectrometry, we demonstrate i

29 ays, mutant analysis, metabolic engineering, isotope labeling and metabolic profiling to capture PFCs

30 (13)C isotope labeling and metabolic studies revealed that the

31 microbial nitrate-respiring communities with isotope labeling and metagenomics to unravel how specifi

32 The combination of stable isotope labeling and molecular network generation was sh

33 y combining microdialysis assays with simple isotope labeling and NMR experiments.

34 sent such an approach utilizing differential isotope labeling and reversed phase liquid chromatograph

35 e PDH-deficient parasites using rapid stable-isotope labeling and show that PDH does not appreciably

36 m of the present study was to combine stable isotope labeling and tandem mass spectrometry for the au

37 We have used isotope labeling and two-dimensional infrared spectrosco

38 k assignments were additionally supported by isotope-labeling and energy-resolved collision induced d

39 Recent breakthroughs in isotope-labeling and pulse sequence techniques have enab

40 Using a combination of MS, isotope labeling, and (1)H and (13)C NMR techniques, we

41 mass spectrometry (MS/MS and MS(3)), stable isotope labeling, and GC-MS analysis, we previously prop

42 Kinetic studies, isotope labeling, and in situ high-resolution mass spect

43 solution mass spectrometry, metabolic stable isotope labeling, and MS/MS-based isotopologue quantific

44 A novel stable isotope labeling approach using (15)N and (18)O was appl

45 Using a metabolomic and stable-isotope labeling approach, combined with transcriptional

46 rotrophic plant tissues, while nonstationary isotope labeling approaches are amenable to the study of

47 quantification methods, specifically stable isotope labeling approaches such as isobaric tags and st

48 )C-MFA advanced methods for measuring stable-isotope labeling are needed.

49 p53 gene-derived DNA duplexes using a novel isotope labeling-based approach.

50 ibutions, are suitable for quantification of isotope-labeling-based studies, and provide additional i

51 molecules (CAMs), measured by pulsed stable isotope labeling by amino acids in cell culture (pSILAC)

52 A two-state stable isotope labeling by amino acids in cell culture (SILAC)

53 hoproteomics that incorporates triple stable isotope labeling by amino acids in cell culture (SILAC)

54 oaches-microarray gene expression and stable isotope labeling by amino acids in cell culture (SILAC)

55 ate (ATP) affinity probe coupled with stable isotope labeling by amino acids in cell culture (SILAC)

56 Additionally, by comparing the stable isotope labeling by amino acids in cell culture (SILAC)

57 oal of the present study was to use a stable-isotope labeling by amino acids in cell culture (SILAC)

58 ication can be achieved by performing stable isotope labeling by amino acids in cell culture (SILAC)

59 Using stable isotope labeling by amino acids in cell culture (SILAC)

60 We combined stable isotope labeling by amino acids in cell culture (SILAC)

61 Quantitative stable isotope labeling by amino acids in cell culture (SILAC)

62 A structure, we used a combination of stable isotope labeling by amino acids in cell culture (SILAC),

63 ng (MRM)-based workflow together with stable isotope labeling by amino acids in cell culture (SILAC),

64 t occur during C. burnetii infection, stable-isotope labeling by amino acids in cell culture (SILAC)-

65 We developed a stable isotope labeling by amino acids in cell culture (SILAC)-

66 Through comparative stable isotope labeling by amino acids in cell culture (SILAC)-

67 By using stable isotope labeling by amino acids in cell culture (SILAC)-

68 Super-stable isotope labeling by amino acids in cell culture (Super-S

69 Stable isotope labeling by amino acids in cell culture and quan

70 eomics analysis was carried out using stable isotope labeling by amino acids in cell culture combined

71 Stable isotope labeling by amino acids in cell culture experime

72 Stable isotope labeling by amino acids in cell culture followed

73 ed in vivo by mass spectrometry using stable isotope labeling by amino acids in cell culture mouse he

74 , we describe systematic quantitative stable isotope labeling by amino acids in cell culture proteomi

75 Quantitative proteomics based on stable isotope labeling by amino acids in cell culture showed t

76 We further performed stable isotope labeling by amino acids in cell culture to analy

77 clarify these issues, we used dynamic stable isotope labeling by amino acids in cell culture to defin

78 expression was revealed using pulsed stable isotope labeling by amino acids in cell culture to ident

79 Proteins, extracted from a SILAC (stable isotope labeling by amino acids in cell culture) labeled

80 SILAC (stable isotope labeling by amino acids in cell culture)-based m

81 ns, we conducted a family-wide SILAC (stable isotope labeling by amino acids in cell culture)-based p

82 00 muM) in conjunction with an SILAC (stable isotope labeling by amino acids in cell culture)-based w

83 Stable isotope labeling by amino acids in cell culture, using p

84 s spectrometry-based technologies and stable isotope labeling by amino acids in cell culture, we ques

85 exes and analyzed their components by stable isotope labeling by amino acids in cell culture-based ma

86 Using stable isotope labeling by amino acids in cell culture-based ma

87 In a stable isotope labeling by amino acids in cell culture-based pr

88 In combination with the use of the stable isotope labeling by amino acids in cell culture-based qu

89 Furthermore, using stable isotope labeling by amino acids in culture (SILAC), we s

90 ic analyses of deletion strains using stable isotope labeling by amino acids in culture identified ot

91 iological light-dark conditions using stable isotope labeling by amino acids quantitative MS.

92 e life span estimates on the basis of stable isotope labeling can vary up to 10-fold among laboratori

93 ing of the milk metabolome based on chemical isotope labeling (CIL) and liquid chromatography mass sp

94 thod was combined with differential chemical isotope labeling (CIL) LC-MS for mapping the metabolome

95 of filling the missing values in a chemical isotope labeling (CIL) LC-MS metabolomics platform.

96 We report a high-performance chemical isotope labeling (CIL) LC-MS method for profiling the ca

97 a metabolomic data set generated by chemical isotope labeling (CIL) liquid chromatography mass spectr

98 Chemical isotope labeling (CIL) liquid chromatography mass spectr

99 human sweat submetabolome based on chemical isotope labeling (CIL) liquid chromatography-mass spectr

100 High-performance chemical isotope labeling (CIL) liquid chromatography-mass spectr

101 a trap column for high-performance chemical isotope labeling (CIL) metabolomic profiling with deep c

102 a method based on high-performance chemical isotope labeling (CIL) nanoflow liquid chromatography ma

103 The application of stable isotope labeling combined with SRM can overcome many of

104 gate this disparity, we generated new stable isotope labeling data in healthy adult subjects using bo

105 Dynamic isotope labeling data provides crucial information about

106 (13)C isotope labeling demonstrates that the reactive electrop

107 temperature dependence is perturbed by heavy isotope labeling, demonstrating a direct link between (p

108 An isotope labeling experiment is used to identify the oxyg

109 measured in vivo using a pulse-chase stable isotope labeling experiment.

110 Isotope labeling experiments and detailed DFT calculatio

111 nection between data obtained from elemental isotope labeling experiments and the well-known compartm

112 mbination was corroborated by intramolecular isotope labeling experiments and theoretical calculation

113 ynaMet for fully automated investigations of isotope labeling experiments from LC-high-resolution MS

114 Using genetic engineering and isotope labeling experiments in combination with infrare

115 he FFC algorithm is able to integrate stable isotope labeling experiments into the analysis and can a

116 trinsic reaction coordinate calculations and isotope labeling experiments of the reactions of D8-cycl

117 Isotope labeling experiments revealed a novel gamma-CH a

118 Through 13C-labeled isotope labeling experiments we elucidate that exosomes

119 Further isotope labeling experiments were carried out to charact

120 Isotope labeling experiments with (15)N-labeled P.A. con

121 Isotope labeling experiments with (18)O(2), (15)NO and N

122 sing results from X-ray crystallographic and isotope labeling experiments, a mechanism for this unusu

123 ovided structural insights and guided stable-isotope labeling experiments, which led to the assignmen

124 erent analytical platforms in the context of isotope labeling experiments.

125 identified metabolites obtained from stable isotope labeling experiments.

126 Furthermore, isotope-labeling experiments demonstrate that the S-meth

127 Here, we describe the use of stable isotope-labeling experiments for studying metabolism und

128 Finally, isotope-labeling experiments rule out the alternative hy

129 A series of isotope-labeling experiments shed light on the bond reor

130 reaction mechanism through kinetic studies, isotope-labeling experiments, (19)F NMR, electrochemical

131 analyzing untargeted LC/MS data from stable isotope-labeling experiments.

132 ted and shown to resolve past mysteries from isotope-labeling experiments.

133 ns (MID) is of great significance for stable isotope-labeling experiments.

134 lubility and ionization, and utilizes stable isotope labeling for MS1 level identification of hydroph

135 Mass spectrometry-based stable isotope labeling has become a key technology for protein

136 Stable isotope labeling has become a well-established such tool

137 ially useful in rapid carboxylic acid carbon isotope labeling, however development toward its applica

138 h the complementary application of oxygen-18 isotope-labeling, HPLC combined with electrospray ioniza

139 (13)C isotope labeling identifies an unexpected bromine migrat

140 measure carbohydrate composition and stable-isotope labeling in algal biomass using gas chromatograp

141 Pulsed Stable Isotope Labeling in Cell culture (SILAC) approaches allo

142 y blue-native gel electrophoresis and stable isotope labeling in cell culture proteomics that the TbS

143 rotein mass spectrometry with dynamic stable isotope labeling in cell culture to achieve a proteome-w

144 eatly propelled by the development of stable isotope labeling in cell cultures (SILAC), a set of stan

145 ly, PAF-C purifications combined with stable isotope labeling in cells (SILAC) quantitation for PAF-C

146 chain (13)C=(18)O will complement main chain isotope labeling in future IR studies of amyloids and in

147 We conclude that stable isotope labeling in healthy humans is consistent with a

148 describe use of quantitative in vivo stable isotope labeling in mammals to accurately compare serum

149 resolution orbital trap was used to quantify isotope labeling in peptides that were obtained from unl

150 f the protein dynamic was studied by protein isotope labeling in the framework of the Variational Tra

151 We made use of stable isotope labeling in tissue culture (SILAC) to identify I

152 Here, we use stable isotope labeling in vivo and proteomics to achieve this

153 With the goal of achieving controllable isotope-labeling in N-alkylated amines, we herein ration

154 Isotope labeling is a powerful technique to probe detail

155 Our study shows that (15)N and (18)O isotope labeling is a useful approach for direct quantif

156 Stable isotope labeling is central to NMR studies of nucleic ac

157 sing sparse NOE data combined with selective isotope labeling is presented.

158 Stable isotope labeling is the state of the art technique for i

159 rticular methodological challenge for stable isotope labeling is to ensure that the label is traceabl

160 Isotope labeling is used for the study of TTR by NMR, ne

161 at this approach, which does not require any isotope labeling, is applicable to ligand-target systems

162 Assisted by the isotope labeling, it was possible to determine the seque

163 A stable isotope labeling kinetics experiment in NHPs was perform

164 ells with (13)C methionine and measuring the isotope-labeling kinetics of both intracellular and extr

165 . coli strains using a differential chemical isotope labeling LC-MS platform.

166 We present a high-performance chemical isotope labeling liquid chromatography mass spectrometry

167 d (UMS) method, in conjunction with chemical isotope labeling liquid chromatography-mass spectrometry

168 c metabolite standards via the use of stable isotope labeling, liquid chromatography mass spectrometr

169 , global untargeted metabolomics, and stable isotope labeling mass spectrometry to identify metabolic

170 Stable-isotope-labeling mass spectrometry involves the addition

171 sponse to 294 biological perturbations using isotope-labeling mass spectrometry.

172 ral parameters for biomembrane systems where isotope labeling may be prohibitive.

173 membranes, but it is challenging to use the isotope labeling method to study interfacial biomolecule

174 Stable isotope labeling-multiple reaction monitoring mass spect

175 Using stable isotope labeling of amino acids in a cell culture phosph

176 By coupling our methodology with stable-isotope labeling of amino acids in cell culture (SILAC),

177 -inducible RTA expression and applied stable isotope labeling of amino acids in cell culture (SILAC)-

178 hed fractions, which were compared by stable isotope labeling of amino acids in cell culture (SILAC)-

179 approaches such as isobaric tags and stable isotope labeling of amino acids in cell culture (SILAC).

180 nical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC).

181 Using stable isotope labeling of amino acids in cell culture and nano

182 ce in combination with in vivo pulsed stable isotope labeling of amino acids in cell culture proteomi

183 We used the stable isotope labeling of amino acids in cell culture proteomi

184 were screened by quantitative SILAC (stable isotope labeling of amino acids in cell culture) co-immu

185 Using a SILAC (stable isotope labeling of amino acids in cell culture)-based p

186 ing)-based transcriptomics and SILAC (stable isotope labeling of amino acids in cell culture)-based q

187 Using stable isotope labeling of amino acids in culture (SILAC) and M

188 w is straightforward, including differential isotope labeling of individual samples and a pooled samp

189 Surprisingly, results obtained with stable isotope labeling of mammals revealed that, in vivo, the

190 Isotope labeling of mESCs revealed that threonine provid

191 antifying metabolic fluxes based on tracking isotope labeling of metabolite within cells.

192 olution mass spectrometry of a double stable isotope labeling of P. nordicum enabled the specific det

193 Stable isotope labeling of peptides is the basis for numerous m

194 We report an enzymatic strategy for "stable isotope labeling of phosphonates in extract" (SILPE) tha

195 method is based on differential (12)C-/(13)C-isotope labeling of polyphenols through derivatization w

196 ntified by isotope shifts, but site-specific isotope labeling of proteins is today possible only for

197 The method was extended to the synthesis and isotope labeling of quinoline and 1,2,3,4-tetrahydroquin

198 al dissociation (HCD)-based fingerprints and isotope labeling of RNA.

199 his protocol describes a strategy for stable isotope labeling of several widely used metal and metal

200 re, this chemistry could be adapted to (13)C-isotope labeling of six pharmaceutically relevant compou

201 omatic ring, and it can be exploited for the isotope labeling of the aldehyde group.

202 By taking advantage of selective isotope labeling of the chains, we studied the role of i

203 Selective (15)N isotope labeling of the cytochrome bo(3) ubiquinol oxida

204 Analysis of constructs with selective isotope labeling of the delta1 methyl groups of isoleuci

205 etic information without the need for stable isotope labeling of the molecules of interest.

206 tive measurements can be performed by stable-isotope labeling of the peptides in the reductive dimeth

207 ts which unambiguously show PET and, through isotope labeling of the protein and the chromophore, are

208 oped system was applied in the synthesis and isotope labeling of two pharmaceuticals, nordazepam and

209 es needed to define fragments, manage stable isotope labeling, optimize collision energy and generate

210 A chemical isotope labeling or isotope coded derivatization (ICD) m

211 that minute spectral shifts induced by metal isotope labeling or temperature changes are detected usi

212 times of 0.4-1.2 h, and can handle arbitrary isotope labeling patterns and data from other types of N

213 s search space can be tailored for different isotope labeling patterns expected in different stable i

214 The isotope labeling patterns of 40 metabolites were obtaine

215 irect evidence for the Fl(N5[O]) species via isotope labeling, proteolytic digestion, and high-resolu

216 n into both virus and host proteins using an isotope-labeling proteomics approach in a model marine c

217 In this work an improved stable isotope labeling protocol for nucleic acids is introduce

218 We present a (13)C-based isotope labeling protocol for RNA.

219 using heavy water (D(2)O) with Raman-stable isotope labeling (Raman-D(2)O), we evaluated the reliabi

220 e between bound and free ligand or on stable isotope labeling, relying instead on a tert-butyl group

221 fication protocol allowed for cost-effective isotope labeling required for a detailed NMR structural

222 icarbonate and propionate) and (15)N-ammonia isotope labeling reveals that cells performing sulfide o

223 Stable-isotope labeling reveals that even severely aggregated e

224 es, along with a judiciously designed stable isotope labeling scheme, to measure atomistic-resolution

225 agnetic resonance combined with an efficient isotope labeling scheme.

226 nkers, replicated analysis with a permutated isotope-labeling scheme.

227 We describe specific isotope-labeling schemes required for working with large

228 onstrates the feasibility and power of using isotope labeling SFG to probe molecular structures of in

229 Peptide quantitation based on stable isotope labeling showed that the surfactant induced 1.5-

230 odeled by the parasite cytoplasm, and stable isotope labeling shows some apicoplast lipids are genera

231 Stable isotope labeling (SIL) techniques have the potential to

232 urally similar internal standards, different isotope labeling strategies, radiolabeling, and predicte

233 An isotope labeling strategy is described for efficient ide

234 As an isotope-labeling strategy for NMR studies, reductive met

235 Isotope labeling studies confirmed that the oxygen atom

236 Isotope labeling studies confirmed the origin of the met

237 We employ a series of kinetic and isotope labeling studies made largely possible by electr

238 Isotope labeling studies rule out a direct four-electron

239 Isotope labeling studies show that a hydrogen is transfe

240 Preliminary kinetics and isotope labeling studies suggest epoxide ring opening as

241 This dogma was recently challenged by stable isotope labeling studies with heavy water, which yielded

242 Isotope labeling studies, coupled with FTIR and CSI-MS,

243 Isotope-labeling studies suggest a mechanism proceeding

244 h the in situ spectroscopic measurements and isotope-labeling studies, support this mode of operation

245 An isotope-labeling study supports an oxidative cross-coupl

246 Degradation-reconstruction approaches for isotope labeling synthesis have been known for their rem

247 We used a combination of genetics, isotope labeling, tandem mass spectrometry, and chemical

248 Here, we used a stable isotope labeling technique ((18)O and (2)H) to determine

249 We also employed a stable-isotope labeling technique to illuminate high-priority m

250 Here, we applied a stable isotope-labeling technique in combination with mass spec

251 Using a stable isotope-labeling technique, we found that dexamethasone

252 Stable isotope labeling techniques for quantitative top-down pr

253 Like popular bottom-up isotope labeling techniques, most top-down labeling meth

254 were analyzed by ESI/qTOF/MS using MS/MS and isotope labeling techniques.

255 roughs during the past decade, especially in isotope-labeling techniques, have enabled NMR characteri

256 currently tenable due to the requirements of isotope labeling, the large size of the proteins, and th

257 en made thanks to the introduction of stable isotope labeling, the state-of-the-art technique for in

258 With the help of site-specific isotope labeling, the topologies of these two structures

259 Together with isotope labeling, these techniques also revealed the bin

260 We use 2D infrared spectroscopy and isotope labeling to monitor the kinetics of fibril forma

261 The approach was enhanced with stable isotope labeling to overcome ambiguities in determining

262 We further used stable-isotope labeling to trace the metabolic dynamics of fatt

263 gen evolution reaction (OER) as evidenced by isotope labeling together with the differential electroc

264 rometry with metabolic [(2)H3]-leucine heavy isotope labeling under divergent conditions.

265 gh sensitivity, we developed cysteine-stable isotope labeling using amino acids in cell culture (SILA

266 ribe an integrated approach combining stable isotope labeling, various protein enrichment and extract

267 The (13)C-isotope labeling was achieved applying a Pd-catalyzed me

268 Using stable isotope labeling, we demonstrated that phosphocholine an

269 s of electronic structure analyses and (18)O isotope labeling, we present a mechanism comprising a co

270 c solvent, and gradient slope) and different isotope labelings were addressed by multiple-factor scre

271 include unpredictable mass shifts following isotope labeling, which impedes analysis of unknown prot

272 ethodology is also highly suitable for (13)C isotope labeling, which was demonstrated through the syn

273 We utilized stable isotope labeling with amino acids (SILAC) in PTECs to co

274 sine profiling method with 'spike-in' stable isotope labeling with amino acids in cell culture (SILAC

275 We report here that pulse-chase stable isotope labeling with amino acids in cell culture (SILAC

276 ent and non-adherent conditions using stable isotope labeling with amino acids in cell culture (SILAC

277 We coupled cell fractionation with stable isotope labeling with amino acids in cell culture (SILAC

278 Using stable isotope labeling with amino acids in cell culture (SILAC

279 irus (IBV) N protein was mapped using stable isotope labeling with amino acids in cell culture (SILAC

280 technique, NeuCode (neutron encoding) stable isotope labeling with amino acids in cell culture (SILAC

281 ss spectrometry and quantification by Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC

282 e surface biotinylation combined with stable isotope labeling with amino acids in cell culture (SILAC

283 In combination with stable isotope labeling with amino acids in cell culture (SILAC

284 a newly established pipeline coupling stable isotope labeling with amino acids in cell culture (SILAC

285 Here, we applied pulsed stable isotope labeling with amino acids in cell culture (SILAC

286 affinity purification), coupled with stable isotope labeling with amino acids in cell culture (SILAC

287 By stable isotope labeling with amino acids in cell culture and ma

288 Using stable isotope labeling with amino acids in cell culture and re

289 us proteins in a given sample (e.g., stabile isotope labeling with amino acids in cell culture is not

290 ovel TBK1/IKKepsilon substrates using stable isotope labeling with amino acids in cell culture phosph

291 this study was generated using SILAC (Stable Isotope Labeling with Amino acids in Cell culture) techn

292 sis of the TIM23 interactome based on stable isotope labeling with amino acids in cell culture.

293 ity-labeled proteins were analyzed by stable isotope labeling with amino acids in culture (SILAC)-LC/

294 In this paper, we describe a stable isotope labeling with amino acids in culture-based quant

295 nce of FTY720 were then identified by stable isotope labeling with amino cells in cell culture, inclu

296 Stable isotope labeling with deuterium oxide, followed by immon

297 Stable isotope labeling with multiple reaction monitoring-mass

298 We couple stable isotope labeling with stimulated Raman scattering micros

299 ced by this fungi, we combined a full stable isotopes labeling with the dereplication of tandem mass

300 asurements of metabolic function from stable isotope labeling within individual organelles in situ.