ライフサイエンスコーパス: kanamycin resistance

1 rom pUC21 (ampicillin resistance) and pUK21 (kanamycin resistance).

2 d replacing it with a gene cassette encoding kanamycin resistance.

3 cation, and genes for rare transfer RNAs and kanamycin resistance.

4 is, which increase its expression and confer kanamycin resistance.

5 utonomously replicating plasmid that confers kanamycin resistance.

6 hia coli-H. pylori shuttle vector conferring kanamycin resistance.

7 cells, and positive clones were selected by kanamycin resistance.

8 A spontaneous kanamycin resistance and capreomycin resistance mutation

9 The transposon introduces both positive (kanamycin resistance) and negative (I-SceI recognition s

10 a bacteriophage T7 promoter, (ii) it confers kanamycin resistance, and (iii) it uses an R6K origin of

11 The loxP sites flank npt, conferring kanamycin resistance, and sacB, which confers sensitivit

12 s to the stable phenotypes of tumorigenesis, kanamycin resistance, and stable beta-glucuronidase (GUS

13 crown gall tumorigenesis, transformation to kanamycin resistance, and stable GUS expression.

14 D were each insertionally inactivated with a kanamycin resistance (aphA) cassette and allelic exchang

15 active hpyIIRM cassette [containing a 1.4 kb kanamycin resistance (aphA) marker], whether such acquis

16 resistance gene may encode spectinomycin or kanamycin resistance based on the expression of aadA or

17 he inactivation of sll0088 by insertion of a kanamycin resistance cartridge in the primary C14S(PsaC)

18 The insertion of a kanamycin resistance cartridge into cbbL resulted in a p

19 e sacB gene of Bacillus subtilis cloned in a kanamycin resistance cartridge.

20 Interruption of por by a kanamycin-resistance cartridge between the codons for M2

21 i, nixA was insertionally inactivated with a kanamycin resistance cassette (aphA) and this construct

22 A kanamycin resistance cassette (Kan(r)) was inserted into

23 ubunit sequence in E. coli by insertion of a kanamycin resistance cassette and sought to construct an

24 , and interruption of the cloned gene with a kanamycin resistance cassette eliminated the overexpress

25 ing sequence was removed and replaced with a kanamycin resistance cassette flanked by two res sites f

26 cells, and disruption of the oapA gene with kanamycin resistance cassette insertion resulted in a si

27 ts providing evidence for integration of the kanamycin resistance cassette into atpD.

28 Insertion of a kanamycin resistance cassette into the 5' end of pifC re

29 BBA74 was disrupted by the insertion of a kanamycin resistance cassette into the coding region.

30 be required for macrophage killing, since a kanamycin resistance cassette introduced into icmS by ge

31 y disruption of the flaA or flaB gene with a kanamycin resistance cassette or by introduction of both

32 Replacement of GSU1501 with a kanamycin resistance cassette produced a similarly defec

33 nC mutant generated by introducing an aphA-3 kanamycin resistance cassette produced CPS with no O-ace

34 An in-frame replacement of eptB with a kanamycin resistance cassette rendered E. coli WBB06 (bu

35 rferi B31 by replacing the BBK32 gene with a kanamycin resistance cassette through homologous recombi

36 strain Ec1a (O1:K1:H7) were replaced with a kanamycin resistance cassette to produce an oxyRS mutant

37 hromosome by homologous recombination with a kanamycin resistance cassette to produce mutant J45-100.

38 ed in which the bca gene was replaced with a kanamycin resistance cassette via homologous recombinati

39 duce a GlnA-deficient mutant of H. pylori, a kanamycin resistance cassette was cloned into the Tth111

40 y of phiBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned

41 Afterwards, the kanamycin resistance cassette was removed from each muta

42 of R. leguminosarum was created by placing a kanamycin resistance cassette within acpXL, the gene whi

43 g rifampin resistance) or a large insertion (kanamycin resistance cassette).

44 d by insertional inactivation of plcR by the kanamycin resistance cassette, aphA3.

45 r hypB (HypB:kan) have been interrupted by a kanamycin resistance cassette.

46 subcloned and mutagenized by insertion of a kanamycin resistance cassette.

47 nsion, was interrupted by the insertion of a kanamycin-resistance cassette between the ferrochelatase

48 phenicol-resistance cassette in place of the kanamycin-resistance cassette in pOX38-tra715 and a muta

49 Insertion of a kanamycin-resistance cassette in the traY gene of the pO

50 sogenic H. pylori uvrB mutant by inserting a kanamycin-resistance cassette into uvrB and verified its

51 optional chloramphenicol-, tetracycline-, or kanamycin-resistance cassettes.

52 ent strain was constructed by insertion of a kanamycin resistance determinant within the ureC gene vi

53 (encoded by ssrA), coupled with a multicopy kanamycin resistance determinant, suppressed both lon ph

54 nstructed in this gene by the insertion of a kanamycin resistance DNA cassette into the chromosome.

55 ked insertion of transposon Tn5 by using the kanamycin resistance encoded by that transposon.

56 ulting MS17 clone possessed erythromycin and kanamycin resistance, flat-wave morphology, and microsco

57 the corresponding gene was disrupted with a kanamycin resistance gene (aphA3) in H. pylori ATCC 4350

58 Integration plasmids carrying a kanamycin resistance gene (kan) that was used to substit

59 A kanamycin resistance gene (kan) was introduced into B. b

60 eliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous reco

61 firmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA.

62 train, E. coli O157:H7 W6-13, by inserting a kanamycin resistance gene cassette (kan) into wcaD and w

63 mutant, which was created by insertion of a kanamycin resistance gene cassette at the 5' region of i

64 e K8.1 open reading frame and insertion of a kanamycin resistance gene cassette within the K8.1 gene.

65 344 (SL1344 trxA), replacing the gene with a kanamycin resistance gene cassette.

66 ch either fimA or orf365 was replaced with a kanamycin resistance gene did not participate in type 2

67 598-bp segment of the gene and inserting the kanamycin resistance gene from Tn903 into the gap.

68 tions of the transposon Tn5 or Tn3-nice or a kanamycin resistance gene in each of these genes abolish

69 The MDV US3 orthologue was replaced with a kanamycin resistance gene in the infectious bacterial ar

70 nalysis revealed there was an insertion of a kanamycin resistance gene in the lgt2 gene of D4, which

71 e vectors into a plasmid containing a mutant kanamycin resistance gene in vitro.

72 ted DBB25, was constructed by insertion of a kanamycin resistance gene into alcA, one of the genes es

73 d one of these fragments hybridized with the kanamycin resistance gene of the plasmid vector.

74 ucted loxP511 transposons contained either a kanamycin resistance gene or no marker.

75 disrupted by insertion of the aphII neomycin-kanamycin resistance gene resulted in the accumulation o

76 disrupted in strain 81-176 by insertion of a kanamycin resistance gene through homologous recombinati

77 LEU2 genes and a transposon-derived neomycin/kanamycin resistance gene were successfully expressed fr

78 es a gC-negative deletion mutant harboring a kanamycin resistance gene, a markerless mutant with the

79 of pDMG21A, a pVT745 derivative containing a kanamycin resistance gene, displayed a structural rearra

80 pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in Esch

81 onstructed by replacing the mazG gene with a kanamycin resistance gene.

82 der gene (originally annotated yfgK) with a kanamycin resistance gene.

83 plants transformed with a vector containing kanamycin-resistance gene (npt) flanked by FRT sites, wh

84 gene product inhibited a transposon-derived kanamycin-resistance gene in both M. smegmatis and M. tu

85 lytic subdomains I-III by the insertion of a kanamycin-resistance gene resulted in slightly delayed,

86 nd negative selection markers, which are the Kanamycin-resistance gene, the sacB gene and temperature

87 plasmids containing mariner transposons and kanamycin resistance genes expressible in B. burgdorferi

88 segment required for psbL editing, chimeric kanamycin resistance genes were constructed containing p

89 ssette was designed so that the luxABCDE and kanamycin resistance genes were linked to form a single

90 gulated expression for the LEU2 and neomycin/kanamycin resistance genes.

91 mined in this study that exhibited low-level kanamycin resistance harbored eis promoter mutations.

92 antify the static and dynamic composition of kanamycin resistance in artificial microbiota to evaluat

93 plication origin, the nptIII gene conferring kanamycin resistance in bacteria, both the right and lef

94 r DNA that adjoins the transposon contains a kanamycin resistance (Kanr) gene.

95 meC in the kan- gene resulted in mutation to kanamycin resistance (KanR).

96 Genetic analysis of the kanamycin-resistance (KanR) trait in >900 independent tr

97 rless chloramphenicol resistance (Cm(r)) and kanamycin resistance (Km(r)) cassettes, respectively, th

98 gI fragment within lob-2A was deleted, and a kanamycin resistance (Km(r)) gene was inserted into this

99 laP, ApR) and pNF2214 carries the Tn903 aph3 kanamycin resistance (KmR) element.

100 Using kanamycin resistance (Kn(r)) insertional alleles of the

101 Insertion of a kanamycin resistance (KnR) cassette at a SalI site in a

102 The kanamycin resistance marker allows efficient direct sele

103 laced in a binary plasmid vectorcontaining a kanamycin resistance marker and a cauliflower mosaic vir

104 nstructs of fbpA and fbpB disrupted with the kanamycin resistance marker OmegaKm and containing varyi

105 omposite transposons containing IS6110 and a kanamycin resistance marker were constructed.

106 structed with the sucA gene interrupted by a kanamycin resistance marker.

107 A gene, which encodes L27, was replaced by a kanamycin resistance marker.

108 When used in tandem with a kanamycin-resistance marker, the cassette was successful

109 These mutations restored high levels of kanamycin resistance not through an improvement in the p

110 slation in the correct reading frame confers kanamycin resistance on the host.

111 Bioluminescence and kanamycin resistance only occur in a bacterial cell if t

112 with both approaches using attP vectors with kanamycin resistance or spectinomycin resistance as the

113 cin, moxifloxacin, amikacin, capreomycin and kanamycin resistance produced similar sensitivities and

114 that suffers mutations during acquisition of kanamycin-resistance results in an overwhelming majority

115 cillin resistance), aphA1-Iab (which encodes kanamycin resistance), strA and strB (which encode strep

116 the ability of these heterodimers to confer kanamycin resistance to Escherichia coli cells was impai

117 hotransferase type II gene, which can confer kanamycin resistance to transgenic plants, represent an

118 The mutations R177S and V198E restored the kanamycin resistance to wild-type levels while maintaini

119 nce of AID and a genetic reversion assay for kanamycin-resistance to investigate the causes of multip

120 d a genetic selection for splicing-dependent kanamycin resistance with no significant bias when six a

121 model successfully predicted the dynamics of kanamycin resistance within artificial microbiota under