ライフサイエンスコーパス: knock-in

1 h non-homologous end joining (NHEJ)-mediated knock-in.

2 orm for corrective CRISPR/Cas9-mediated gene knock-in.

3 whether ARL13B is required within cilia, we knocked in a cilia-excluded variant of ARL13B (V358A) an

4 In animal experiments knocking in a glutamic acid (S1530E) in DEPDC5, a phosph

5 , we engineered a humanized gene, hmTert, by knocking-in a 47-kilobase hybrid fragment containing the

6 d, we generated a murine reporter strain by "knocking-in" a fluorochrome, tandem-dimer Tomato (tdTom)

7 high efficiency generation of both indel and knock-in allele can be achieved by electroporation of sm

8 In contrast, the Igh (Ter5HDeltaTM) knock-in allele, which generated stable IgHR but no dete

9 C181S mutation into a conditional NrasG12D "knock-in" allele.

10 electroporation for the generation of simple knock-in alleles using single-stranded oligodeoxynucleot

11 Using this mouse model denoted knock-in alpha2 (KI alpha2), our electrophysiological st

12 tope in BG505 SOSIPv4.1 trimer immunogens by knocking in an N-linked glycan at residue 241.

13 taGAG/+) mice are shared with Thap1 (C54Y/+) knock-in and Gnal (+/-) knock-out dystonia models and to

14 ble CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell

15 d characterizing a panel of recombinant TbD1-knock-in and knock-out strains and comparison with clini

16 nditional gene expression, RNA interference, knock-in and knock-out, lack sufficient spatiotemporal a

17 response to a neoepitope when this site was knocked in and the N356-glycan site knocked out.

18 pments of the technique for highly efficient knock-in, and demonstrate pros and cons.

19 y development that leverages the transgenic, knock-in, and knock-out variants of mouse models of huma

20 es on-target insertion and deletion rates in knock-in applications.

21 Using a knock-in approach at the hypoxanthine-guanine phosphorib

22 Using a knock-in approach that combines MMP-resistant or ADAMTS-

23 or by a newly developed CRISPR/Cas9-mediated knock-in approach, we also studied the importance of cri

24 d nAChRs introduced in the mouse by a CRISPR knock-in are regulated and expressed like the native pro

25 ulted in a significantly higher rate of gene knock-in as compared to the HR template (37.0% and 13.8%

26 2/3 double KO [DKO]), TTP-S52A-S178A (TTPaa) knock-in, as well as combined MK2 KO/TTPaa and MK2/3 DKO

27 es in mice in which p.Asp395Gly VCP mice was knocked in, as compared with injected wild-type mice.

28 that synthetic HDR templates can be used to knock-in bacterial nitroreductase (ntr) to facilitate li

29 e in which the alkaline phosphatase gene was knocked-in (Badea et al. [Neuron] 2009;61:852-864; Badea

30 on with a D3-specific S-35 riboprobe in FAAH knock-in C385A mice confirmed significantly increased D3

31 transients, topology of fluorescently tagged knock-in Ca(2+) channels, and Ca(2+) channel-synaptic ve

32 hich suppresses somatic expansion of the Htt knock-in CAG repeat, blocked the Fan1 knock-out-induced

33 ethods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently.

34 approach by assessing the function of these knock-in cell lines in cytokinesis, receptor degradation

35 quencing and RNA sequencing studies of S180A knock-in cells demonstrated enhanced DNA binding and inc

36 A mouse harboring the knock-in Clcn7 variant exhibited hypopigmentation, hepat

37 -type (WT), Nlrp3(-/-) , or Nlrp3(L351PneoR) knock-in crossed to inducible (estrogen receptor Cre-Cre

38 nvestigated the impact of wild-type (WT) and knock-in Cx43 with serine to alanine mutations at the pr

39 UQCRC1 p.Tyr314Ser knock-in Drosophila and mouse models exhibit age-depende

40 The knock-in efficiency for a 1.8 kb gene was contrasted whe

41 ng (HMEJ) approach has been shown to improve knock-in efficiency in non-dividing cells and to harness

42 Additionally, more than a third of the knock-in embryos (36.9%) were non-mosaic.

43 his question, we generated allelic series of knock-in Fgfr1 and Fgfr2 mouse strains, carrying point m

44 generated mouse embryonic stem cells (ESCs) knocked in for a slow elongating form of RNAPII We show

45 Here we characterized a mouse model knocked-in for a frameshift mutation in RYR1 exon 36 (p.

46 We created a mouse model knocked-in for the Q1970fsX16+A4329D RYR1 mutations, whi

47 In a knock-in Fus-ALS mouse model, we identified postsynaptic

48 ting tools have simplified the generation of knock-in gene fusions, yet the prevalent use of gene-spe

49 zygotic CRISPR/Cas9 injections to generate a knock-in GFP reporter mouse at the Gdf11 locus.

50 s including transgenic FLAG-alpha(2a)ARs and knock-in HA-alpha(2a)ARs, we investigated the in vivo sp

51 ng (sNUSP), a method that employs NEDD8 R74K knock-in HEK293 cells, allowing discrimination of endoge

52 tified rare variants using CRISPR/Cas9-based knock-in human dopaminergic SH-SY5Y cell lines, Drosophi

53 Using the knock-in humanized mouse model of SCD and SCD patient bl

54 ormation to TG2, we generated immunoglobulin knock-in (Ig KI) mice that express a prototypical celiac

55 sed by post-growth surface contamination and knocking-in impurity species from the surface.

56 The major weakness of most knock-in JAK2V617F mouse models is the presence of the J

57 Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been ext

58 s to rhesus macaques and bnAb immunoglobulin knock-in (KI) mice expressing diverse precursors of CD4

59 ockout (KO) mice lacking cPLA(2)alpha and in knock-in (KI) mice in which endogenous cPLA(2)alpha was

60 Nox5 knock-in (KI) mice represent the first mechanism-based a

61 pression pattern in sensory neurons of S801A knock-in (KI) mice was comparable to that in WT controls

62 Psen1 KO mice and knock-in (KI) mice with homozygous FAD-associated L435F

63 similarly in affected tissues of mutant C10 knock-in (KI) mice, demonstrating that L-OPA1 cleavage i

64 to truncate full-length mutant HTT in HD140Q knock-in (KI) mice, we show that exon 1 HTT is stably pr

65 HTT from brains of Huntington's disease (HD) knock-in (KI) mice.

66 ery of function following ischemic injury, a knock-in (KI) mouse expressing the UCHL1 C152A mutation

67 We sought to develop a novel Nlrp3 knock-in (KI) mouse model of CAPS to study amyloidosis,

68 dent estrogenic-genes using the AF-2 mutated knock-in (KI) mouse model, AF2ERKI.

69 Mutagenesis of exon 2 in Knock-in (KI) mouse models of the R47H variant introduce

70 B-cell receptor (BCR) knock-in (KI) mouse models play an important role in vac

71 S tau transgenic mice on either a human ApoE knock-in (KI) or ApoE knockout (KO) background, here we

72 blast activation, atrial fibrosis, and AF in knock-in (KI) rats.

73 Because a homozygous knock-in (KI) strain for the mP215L mutation homologous

74 Intriguingly, a knock-in (KI) Tonsl mouse model leads to embryonic letha

75 cies in spermatogenesis, we generated a GRTH Knock-In (KI) transgenic mice with the R242H mutation.

76 ent of the neuronal endolysosomal network by knocking in (KI) an engineered ascorbate peroxidase (APE

77 Using the novel PlxnA4(KRK-AAA) knock-in male and female mice, generated by CRISPR/cas9,

78 To test this, we generated knock in mice carrying a mutation (Bmp7(R-GFlag)) that p

79 of genetic cooperativity in the brain of HD knock-in mice (allelic series of Hdh mice).

80 lication of a similar strategy to Nl3(R451C) knock-in mice (genetic cue) also caused successful recov

81 Wild-type (WT) and mutated TrkB knock-in mice (Ntrk2tm1Ddg/J) with impaired BDNF signali

82 teraction in bone remodeling, we created OPG knock-in mice (opg (AAA) ).

83 In mouse studies, phospholemman knock-in mice (PLM(3SA); phospholemman [FXYD1] in which

84 Osteoclasts from the knock-in mice also showed reduced Src activity.

85 llenged wild-type mice but not in Nlrp3L351P knock-in mice and ex vivo-stimulated mutant macrophages.

86 Moreover, we generated cks2(cks1/cks1) knock-in mice and found that CKS1 can compensate for CKS

87 ated from "redox dead" (Cys17Ser) PKARIalpha knock-in mice and their wild-type littermates.

88 ing HIV-1 antibodies in human bnAb precursor knock-in mice and wild-type macaques vaccinated with imm

89 hodopsin level in the retinae of Rho(P23H/+) knock-in mice at 1 month of age.

90 Knock-in mice bearing a mutation of MET caspase cleavage

91 CHIP-S20E knock-in mice better clear ubiquitinated proteins and ar

92 We showed that knock-in mice carrying N88K, a prevalent CMS mutation of

93 we generated and characterized heterozygous knock-in mice carrying the human R100W-mutated allele (N

94 To assess the function of CCN1 in vivo, knock-in mice carrying the integrin alpha6beta1-binding-

95 Knock-In mice carrying the SJ1 patient mutation (SJ1(RQ)

96 sed in lesions of ApoE(-) (/) (-) /P387-TSP4 knock-in mice compared to ApoE(-) (/) (-) mice.

97 oclast number were significantly elevated in knock-in mice compared with wild-type mice.

98 Heterozygous and homozygous R405W-desmin knock-in mice develop both a myopathy and a cardiomyopat

99 Female TDP-43(Q331K) knock-in mice displayed increased weight relative to wil

100 Adult heterozygous knock-in mice displayed smaller neuromuscular endplates

101 lts indicate that while female TDP-43(Q331K) knock-in mice do display progressive behavioural phenoty

102 Here, R1441C and G2019S knock-in mice enabled thorough evaluation of dendritic s

103 y sequences elicited in human bnAb precursor knock-in mice encoded functional improbable mutations cr

104 In naive CD4bs, unmutated common ancestor knock-in mice Env(+)B cell clones develop anergy and par

105 Y. pestis-infected Mefv(M680I/M680I) FMF knock-in mice exhibited IL-1-dependent increased surviva

106 Compared with Raptor(+/+) mice, Raptor(D/D) knock-in mice exhibited smaller livers and hearts, and a

107 The knock-in mice express low levels of the resulting trunca

108 Mice with myeloid-specific Ccn1 deletion and knock-in mice expressing CCN1 unable to bind alpha(v)bet

109 We further show that knock-in mice expressing pV1 sensed heat normally but su

110 avage by hydroxylamine, and macrophages from knock-in mice expressing the E3 ligase-inactive HOIL-1[C

111 Here we report that knock-in mice harboring a cysteine-to-alanine substituti

112 further generated and analyzed R405W-desmin knock-in mice harboring the orthologous form of the huma

113 Moreover, whereas heterozygous knock-in mice have a normal life span, homozygous animal

114 rease Trem2 expression, and in parallel from knock-in mice heterozygous or homozygous for the Trem2 R

115 In anti-thymocyte/Thy-1 autoreactive BCR knock-in mice lacking self-Thy-1 ligand, immunoglobulin

116 nes (ISGs), we hypothesized that STING N153S knock-in mice may develop more severe autoinflammatory d

117 her studies of male and female TDP-43(Q331K) knock-in mice may help to unravel the mechanisms underly

118 ecapitulated in the hearts from R405W-desmin knock-in mice of both genotypes.

119 Foxf1(WT/S52F) knock-in mice recapitulated histopathologic findings in

120 Sustained GSK3 activity in GSK3(S/A) knock-in mice reportedly accelerates peripheral nerve re

121 Heterozygous green fluorescent protein (GFP)-knock-in mice revealed rapid induction of gene expressio

122 For example, S180A knock-in mice showed markedly reduced spontaneous tumori

123 ed and survival of heterozygote Tsc2(S1365A) knock-in mice subjected to the same stress is improved b

124 nsive wild-type mice, but not C42S PKG1alpha knock-in mice that are resistant to disulfide activation

125 elicited antibody responses in macaques and knock-in mice that exhibited the mutational patterns, st

126 Homozygous knock-in mice that express a phosphorylation-silencing m

127 Here we show that knock-in mice that express catalytically inactive caspas

128 ruses, we generated heterozygous STAT1 R274W knock-in mice that have a frequently reported STAT1 muta

129 We previously generated STING N153S knock-in mice that have a human disease-associated gain-

130 Here, we use Cbfb-MYH11 knock-in mice to show that IL1RL1 is expressed by cell p

131 The blood pressure of wild-type and C42S knock-in mice was assessed using implanted telemetry pro

132 The prevailing phenotype of Trem2 R47H knock-in mice was decreased expression levels of Trem2 i

133 HSC-specific Nlrp3(L351P) knock-in mice were generated by crossing transgenic mice

134 ed in a child with this disorder, and Grin2a knock-in mice were generated to model and extend underst

135 ivo, new phosphorylation-deficient p53-S180A knock-in mice were generated.

136 f C674 oxidation on apoptosis in vivo, SERCA knock-in mice were subjected to chronic ascending aortic

137 In humans and knock-in mice with a loss of function BDNF SNP (Val66Met

138 confirmed cognitive deficits in Atxn1154Q/2Q knock-in mice with brain-wide expression of mutant ATXN1

139 To this end, we generated knock-in mice with Cre-conditional expression of a cytid

140 CE1 S-palmitoylation through the analysis of knock-in mice with cysteine-to-alanine substitution at t

141 Using knock-in mice with disrupted ZBP1 nucleic acid-binding a

142 e the dramatically different manifestations, knock-in mice with human HD-AIP missense mutations c.500

143 ouse model obtained by crossing JAK2V617F/WT knock-in mice with PF4iCre transgenic mice.

144 ecific S-35 riboprobe were carried out in 30 knock-in mice with the FAAH C385A polymorphism (20/30 AC

145 In Npm1c/Dnmt3a mutant knock-in mice, a model of AML development, leukemia is p

146 er validate these results, we also used Q175 knock-in mice, an animal model of Huntington's disease i

147 igher (+)-PHNO binding in both humans and in knock-in mice, and this effect was restricted to D3 sele

148 In myocytes from SERCA knock-in mice, basal SERCA activity and SR calcium conte

149 expression, either by siRNA or from the R47H knock-in mice, displayed a similar phenotype.

150 sess learning and memory in homozygous Kv7.2 knock-in mice, Kv7.2(S559A), which show reduced M-curren

151 In ApoE(-) (/-) /P387-TSP4 knock-in mice, lesions size measured by Oil Red O did no

152 In knock-in mice, Msx2 gene was replaced by n-LacZ gene enc

153 In SCA17 knock-in mice, mutant TBP inhibits SP1-mediated gene tra

154 Using knock-in mice, we also find that induced expression of D

155 Using sorted B cells from V(H)B1-8 knock-in mice, we evaluated B-1b, marginal zone, and fol

156 In bnAb precursor knock-in mice, we isolated a vaccine-elicited monoclonal

157 ed in tumors grown in the Ifnar1(S526A) (SA) knock-in mice, which are deficient in IFNAR1 downregulat

158 ed glutamatergic transmission in FDD and FBD knock-in mice, which carry pathogenic FDD and FBD mutati

159 he NSC's physiological role in corresponding knock-in mice.

160 ailure, all of which were inhibited in SERCA knock-in mice.

161 , we generated phospho-deficient GluA2 Y876F knock-in mice.

162 d serum ceramide levels in Cln3 (Deltaex7/8) knock-in mice.

163 ing human fecal transfers into ATG16L1 T300A knock-in mice.

164 CRF(2)(-/-), and MC-deficient Kit(W-sh/W-sh) knock-in mice.

165 ased Th17 cells selectively in ATG16L1 T300A knock-in mice.

166 ent increased survival relative to wild-type knock-in mice.

167 liorates Purkinje cell degeneration in SCA17 knock-in mice.

168 tor dysfunction in UQCRC1 p.Tyr314Ser mutant knock-in mice.

169 lation and elicit behavioral changes in SOUL knock-in mice.

170 bility of the expanded CAG repeat in Htt CAG knock-in mice.

171 Ddr2 during tooth formation using Ddr2-LacZ knock-in mice.

172 variants and in cells derived from P387-TSP4 knock-in mice.

173 peared to be reduced during periodontitis in knock-in mice.

174 g mutant presenilin 1 (PS1) or PS1(M146V/+) "knock-in" mice leads to a complete rescue of deficits in

175 We generated a knock-in model carrying an amino acid substitution (V154

176 A mouse knock-in model expressing the patient mutation N1768D re

177 e early stage motor deficits in a Drosophila knock-in model of ALS that best replicates gene dosage i

178 ent in this study, to our knowledge, a novel knock-in model of constitutive active CD11b in mice.

179 (RAD18) and a non-ubiquitylable (PCNA K164R) knock-in model recapitulate the observed SL induction.

180 Previously, we used a mouse knock-in model to show that Cbfb-MYH11 induces changes i

181 rom 3 species: a mouse huntingtin (Htt) gene knock-in model, a transgenic HTT sheep model, and humans

182 ALS, we made use of a Drosophila Sod1 (G85R) knock-in model, in which all cells harbor the disease al

183 ing mTORC1 activity is not altered in either knock-in model.

184 i-GONAD can also generate knock-in models containing up to 1-kb inserts when singl

185 r both knock-out and multiple user-specified knock-in modifications from one or many edited samples.

186 Transgenic mice with a knock-in mole CYP17A1 enhancer or overexpressing FGF9 sh

187 AT in the medial forebrain bundle (MFB) of a knock-in mouse (both sexes) were analyzed using confocal

188 Here, using a newly generated, tagged Gli3 knock-in mouse (Gli3(TAP) ), we performed proteomic anal

189 a novel mouse model of CDD, the Cdkl5(R59X) knock-in mouse (R59X), to investigate changes in synapti

190 n Erdj5 knockout mouse crossed with the P23H knock-in mouse and by adeno-associated viral (AAV) vecto

191 Here we developed a combined knock-in mouse and chemogenetic approach for cell-type-s

192 acterized a nonphosphorylatable nNOS(S1412A) knock-in mouse and evaluated its enteric neurotransmissi

193 ssues, we generated a Copa(E241K/+) germline knock-in mouse bearing one of the same Copa missense mut

194 We previously generated a knock-in mouse carrying the corresponding CMT2O mutation

195 ineage-specific enhancers using an enCRISPRi knock-in mouse establish in vivo evidence for lineage-re

196 By utilizing a knock-in mouse expressing a chimeric protein comprised o

197 intact brain and acute brain slices from the knock-in mouse expressing epitope-tagged DAT.

198 Using a new knock-in mouse for activity-dependent genetic labeling (

199 ARIalpha disulfide formation in "redox dead" knock-in mouse hearts resulted in larger infarcts (2-fol

200 Here, we used a knock-in mouse in which the p53-Mdm2 negative feedback l

201 Using a human CTLA-4 knock-in mouse lacking FcgammaR function, antitumor effi

202 We found that a knock-in mouse lacking the CTD of GluA1 expresses normal

203 Here, we use a knock-in mouse line (mice expressing Angpt2(443)), a gen

204 Using a Grp-Cre knock-in mouse line, we show that the upper epidermis of

205 To study T cells in vivo, we developed a new knock-in mouse line, which expresses a fusion protein of

206 imensional RNA-seq and proteomic data from a knock-in mouse model (Hdh mice) of Huntington's disease

207 sed an alpha9 cholinergic nicotinic receptor knock-in mouse model (of either sex) with enhanced media

208 associations were replicated in the Q175 Htt knock-in mouse model (p = 6.0 x 10(-8)) and in the trans

209 Here, we generated a knock-in mouse model expressing a mutation that abolishe

210 We have developed a novel knock-in mouse model harboring heterozygous (Tubb4a(D249

211 In a knock-in mouse model of a loss of function human variant

212 senilin-1 (APP/PS-1) human transgenic double-knock-in mouse model of AD.

213 in cerebellar cell lines derived from a CLN3 knock-in mouse model of human Batten disease and control

214 We used an isogenic germline knock-in mouse model to study the effects of RIT1 mutati

215 progression of breast cancer, we generated a knock-in mouse model where binding of Pygo2 to H3K4me(2/

216 role in disease pathogenesis, we generated a knock-in mouse model with NB disruption mediated by 2 po

217 h TYK2 kinase domain mutants and a TYK2 980I knock-in mouse model, we demonstrate that this single am

218 dy focuses on the MH susceptible G2435R-RYR1 knock-in mouse model, which is the murine equivalent of

219 filin-2 with the human disease, we created a knock-in mouse model.

220 phosphorylation, we generated phosphomimetic knock-in mouse models by replacing conserved serines 171

221 enetic studies, Fam83h knockout and mutation-knock-in mouse models indicated that FAM83H does not ser

222 Knock-in mouse models of PM revealed defects in embryoni

223 evidence of metabolic defects in G2435R-RYR1 knock-in mouse muscle under basal conditions.

224 We recently described a knock-in mouse strain in which the substitution of 2 ami

225 ciated mutation induces skin inflammation, a knock-in mouse strain was generated that allows tamoxife

226 onventional hFcRn transgenic mice and in new knock-in mouse strains.

227 The C5aR2 knock-in mouse will help to reliably track and condition

228 aches, including a nonphosphorylatable STIM1 knock-in mouse, that STIM1 phosphorylation is not requir

229 We previously characterised a TDP-43(Q331K) knock-in mouse, which demonstrated behavioural phenotype

230 we performed mechanistic studies in two Pten knock-in murine models, distinct from each other in cell

231 In addition, we show that the efficiency of knock-in mutagenesis can be further increased by electro

232 The phosphomimetic Raptor-S606D knock-in mutant led to a reduction in cell size and prol

233 ke and anxiety-like behaviors in Hdh(+/Q111) knock-in mutant mice by using a battery of behavioral te

234 Both PRAP1-deficient and E85V knock-in mutant mice fed a chow diet manifested an incre

235 amine- and cAMP-regulated phosphoprotein 32) knock-in mutant mouse was generated to analyze the role

236 The conditional knockout of MYPT1 or the knock-in mutation T853A in mice had no effect on muscari

237 A knock-in mutation that blocks E2F1 acetylation abolishes

238 We show that mice with DAD knock-in mutations have memory deficits, reduced anxiety

239 m autoactivation in mice is possible through knocking in mutations that render trypsinogen sensitive

240 n vitro, this mutation increased adhesion of knock-in neutrophils to fibrinogen and decreased neutrop

241 e generated mice with smooth muscle-specific knock in of the hepcidin-resistant isoform fpn C326Y.

242 Knock-in of a human KGKY sequence resulted in a reductio

243 rmed as the mechanism of protection by using knock-in of a mutant p27 that was unable to bind to Skp2

244 In Drosophila, knock-in of a proofreading deficient mtDNA polymerase (P

245 e foundation for curing hemophilia A by NHEJ knock-in of BDDF8 at Alb introns after AAV-mediated deli

246 r dsDNA templates, we successfully performed knock-in of fluorophores at multiple genomic loci and de

247 , we generated fetuses with a liver-specific knock-in of fpnC326Y or knockout of the hamp gene.

248 DNMT's activity, we engineered combinatorial knock-in of human DNMT genes in Komagataella phaffii, a

249 end joining (NHEJ)-based strategies for the knock-in of markers, figure out recent developments of t

250 CRISPR knock-in of mScarlet into the daf-2b genomic locus confi

251 and a robust murine model of ACM (homozygous knock-in of mutant desmoglein-2 [Dsg2(mut/mut)]) that re

252 hanges in metabolism following a single copy knock-in of mutant PIK3CA (H1047R) in the MCF10A cell li

253 ration of loss of function mutations and the knock-in of specific alleles using DNA templates and hom

254 we found that mice with genetic knockout and knock-in of the Fxn gene (KIKO mice) developed exercise

255 harboring a paternally-inherited ubiquitous knock-in of the HAMP-resistant fpnC326Y.

256 Mice with knock-in of the human GRTH mutation are sterile and lack

257 -Cas9 genome editing to make a single allele knock-in of the most commonly mutated amino acid residue

258 Heterozygous knock-in of the mutations or deletion of one copy of TIN

259 We generated mice with knock-in of the p.K24R mutation (called T7K24R mice), wh

260 Mice with heterozygous knock-in of the SERCA C674S mutation were subjected to c

261 le and highly efficient generation of double knock-in of two different CARs in the same T cell.

262 erimental and therapeutic approaches such as knock-in/out strategies are more suitable for binary con

263 P1 knock-in premegakaryocyte/erythroid progenitors demonstr

264 urther development of approaches to generate knock-in protein fusions via generic donors that do not

265 s oligonucleotides for use with the GeneWeld knock-in protocol; (ii) MEDJED, a machine learning metho

266 tant in thyroid hormone receptor beta(PV/PV) knock-in (PV) mice that develop metastatic thyroid cance

267 of 15 patients with OS and the 129Sv/C57BL/6 knock-in Rag2(R229Q/R229Q) (Rag2(R229Q)) mouse model.

268 ating inflammation and cardiac fibrosis in a knock-in rat model.

269 double-stranded DNA mediated large size gene knock-in rates, systematically reduces off-target insert

270 otective/pathogenic mechanisms, we generated knock-in rats carrying either the protective (App(p)) or

271 y SAD pathogenesis, we generated Trem2(R47H) knock-in rats, which carry the SAD risk factor p.R47H va

272 stage in fetal gonads using Prdm14 H2BVenus knock-in rats.

273 graft survival is significantly prolonged in knock-in recipients and sustained recipient expression o

274 elta was made resistant to ROS activation by knock-in replacement of regulatory domain methionines wi

275 sparse random recombination of a conditional knock-in reporter allele expressing alkaline phosphatase

276 Here, we generated the HOXA9-mCherry knock-in reporter cell lines to dissect HOXA9 regulation

277 Using a CDX2(eGFP) iPSC knock-in reporter line to track the emergence of hindgut

278 Using an MYH6:mCherry knock-in reporter line, we developed a protocol to gener

279 Here, we use two novel hPSC knock-in reporter lines expressing GFP under the LMX1A a

280 cytometry as well as the use of Cldn14-lacZ knock-in reporter mice confirmed increased Cldn14 expres

281 l level, we have generated a novel strain of knock-in reporter mice, termed GATIR, by inserting an ex

282 Here, we show that a knock-in reporter mouse for the stem cell gene Musashi 2

283 In this study, we used mice bearing a knock-in sarcomeric mutation, which is exhibited in huma

284 After cysteine knock-in strains have been generated, this protocol can

285 We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, gene

286 sed a CRISPR/Cas9-mediated homology-directed knock-in strategy to generate missense mutants and analy

287 as induced for 30 days in wild-type and Msx2 knock-in Swiss mice using Porphyromonas gingivalis infec

288 Using murine knock-in technology and quantitative PCR, immunoblotting

289 We also attempted to knock in the N289-glycan to block the sole autologous NA

290 We demonstrate that we can "knock in" this ability for TLR9 amplification in membran

291 We generated a novel mouse line harboring a knock-in Thr607 to Ala (Kv4.2TA) mutation that abolished

292 Here, we used CRISPR/Cas9-mediated knock-in to tag the critical ESCRT-I component tumor sus

293 ally encoded vinculin tension sensor that we knock-in to the mouse genome, we show that cortical forc

294 els in which an expanded CAG repeat had been knocked in to the endogenous Htt gene.

295 transgene Prom1(cre-ert2-nlacz) , which was knocked in to the Prom1 gene locus, thus creating functi

296 The half-life of the knocked-in transcript was shorter than that of the endog

297 kinase activity with 1-NM-PP1 in TrkA(F592A)-knock-in (TrkA-KI) eosinophils.

298 e precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway

299 parison of inserts introduced through CRISPR-knock in, with the aim of optimising this approach for s

300 with an ERalpha DNA-binding domain mutation knocked in (WT/KI) to produce WT, ERalpha KO, or ERalpha