ライフサイエンスコーパス: knockout mutant

1 mitochondrial calcium uptake capacity in the knockout mutant.

2 opied an ACTIN DEPOLYMERIZING FACTOR4 (ADF4) knockout mutant.

3 Physcomitrella patens, we generated a pgrl1 knockout mutant.

4 and root epidermal protoplasts of the Atann1 knockout mutant.

5 ein import-defective phenotypes of an hsp93V knockout mutant.

6 faced core gp120 probe and its cognate CD4bs knockout mutant.

7 revealed that this strain is not a true prrA knockout mutant.

8 al surface of the wild type but not the OppA knockout mutant.

9 plete absence of tassel branches in the bif2 knockout mutant.

10 in five times faster than into the fcbT1T2T3 knockout mutant.

11 ignaling might be affected in the Lpp double-knockout mutant.

12 n system were not affected in the lpp double-knockout mutant.

13 upernatant of cells infected with a cmvIL-10-knockout mutant.

14 ild type was lost in the LHY and CCA1 double knockout mutant.

15 antly reduced in a myosin xik xi1 xi2 triple-knockout mutant.

16 nsitive phenotype of the Arabidopsis mtp11-3 knockout mutant.

17 p in resistance, we created double NspA/FHbp knockout mutants.

18 oth responses are largely diminished in shyg knockout mutants.

19 were determined via SMRT sequencing of gene knockout mutants.

20 core proteins and their corresponding CD4bs knockout mutants.

21 pendent of target size, exclusively recovers knockout mutants.

22 y particle bombardment to engineer 1201 gene knockout mutants.

23 ploid-convertible heterozygous diploid yeast knockout mutants.

24 and XI-2/XI-B, using single and double gene-knockout mutants.

25 o molybdoenzymes in Arabidopsis thaliana PCD knockout mutants.

26 were studied by growth phenotype analysis of knockout mutants.

27 absence of LIP8 expression in the homozygous knockout mutants.

28 athogenicity island (PAI) mutants, or double knockout mutants.

29 ignal transduction pathway is present in the knockout mutants.

30 was slower than that of wild type and single knockout mutants.

31 he phenotype of Fz5/Fz8 compound conditional knockout mutants.

32 ed genes were shown to have vital roles with knockout mutants.

33 Expression of VviCCC in an Arabidopsis ccc knockout mutant abolished the mutant's stunted growth ph

34 A mur3 knockout mutant also resists infection by H. parasitica

35 s of CpRbp1 were performed by constructing a knockout mutant and analyzing the resulting heterokaryon

36 lso observed in a DeltapauA2DeltaphoU double knockout mutant and complemented by the wild-type phoU g

37 organisms in acid was absent in the alpha-CA knockout mutant and in the presence of acetazolamide, al

38 However, both the amyR knockout mutant and over-expression strains showed reduc

39 ng molecular and biological responses of the knockout mutant and overexpression lines of AtRAP upon b

40 Analysis of a thaxtomin dual module knockout mutant and single module knockout mutants revea

41 er of flagella was similar in the lpp double knockout mutant and the WT serovar Typhimurium.

42 Through a series of markerless gene knockout mutants and complementation tests, specific com

43 tightly controllable conditional CL synthase knockout mutants and identified a set of novel CL-depend

44 PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-int

45 udy system, we employ reverse genetic tools (knockout mutants and RNA interference) to demonstrate th

46 Here, we characterized the SMT knockout mutants and their complemented strains in Leish

47 Knockout mutants and transcriptomic analysis of B. subti

48 ene and verified its identity by analysis of knockout mutants and transformation.

49 Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWR

50 d transporter1 and ferric chelate reductase2 knockout mutants and was prioritized over immunity, as h

51 ies using gp120 core protein, single-residue knockout mutants, and chimeric viruses revealed that G37

52 n, we generated dcl2drb4 and dcl4drb4 double knockout mutants, and subjected them to infections with

53 ic screen for suppressors of chlorotic tic40 knockout mutant Arabidopsis thaliana plants.

54 ae and that several single and multiple gene-knockout mutants are computationally predicted to improv

55 cdkc;2 and cyct1;5 knockout mutants are highly resistant and cdkc;2 cyct1;5

56 Single fps1 and fps2 knockout mutants are phenotypically indistinguishable fr

57 The upp knockout mutants are unable to grow photoautotrophically

58 S-A-c-myc or 35S::AtSCS-B-c-myc in the scs-1 knockout mutant background revealed that, in planta, bot

59 larvae of B. mori infected with a BmNPV ptp knockout mutant (BmPTPD).

60 ACC2 knockout mutants, by contrast, are hypersensitive.

61 emarkably, in some plant species, homozygous knockout mutants can be produced in a single generation.

62 iae yeast strain, and Mn sensitivity of mtp8 knockout mutants characterized the protein as a vacuolar

63 Leaf senescence is delayed in the SAG113 knockout mutant compared with that in the wild type, sto

64 ssing the two genes in trans in the two-gene knockout mutant complemented full virulence.

65 Seed permeability assays of knockout mutants corresponding to signature genes reveal

66 in and an isogenic transcriptional regulator knockout mutant (covR) also were compared.

67 tored in the wild-type strain, as well as in knockout mutants defective in a variety of methylotrophy

68 accine strain measles virus (MV) or isogenic knockout mutants deficient for either V (V(ko)) or C (C(

69 -rbt1), its control (DAY286), homozygous rbt knockout mutants deficient in rbt1 (BCa7-4) or rbt4 (BCa

70 t mutant, DeltaaebG V. harveyi, and the NRPS knockout mutant, DeltaaebF V. harveyi, do not produce am

71 The FACL knockout mutant, DeltaaebG V. harveyi, and the NRPS knoc

72 The knockout mutant Deltamnx showed increased sensitivity to

73 The construction of nonpolar markerless phpP knockout mutants (DeltaphpP) in two pathogenic strains,

74 n from Taiwan, together with an isogenic PVL-knockout mutant (Deltapvl) and complemented PVL-positive

75 wild-type S. aureus cell but not the double knockout mutant DeltatarM/S, which lacks both alpha- and

76 e biosynthetic monomers obtained from a gene knockout mutant (DeltaUV_2091) of V. virens.

77 ve cell-wall analytical assays of the double knockout mutant demonstrated reduced levels of pectin me

78 t construction and examination of single ccm knockout mutants demonstrated that membrane insertion an

79 Although the pir2 knockout mutant did not display altered ABA response, th

80 Interestingly, the knockout mutant did not exhibit enhanced plant growth in

81 Hpse2 knockout mutants display a distended bladder (megacystis

82 mekk1 knockout mutants display a severe dwarf phenotype, const

83 e role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and

84 Interestingly, mri-knockout mutants display spontaneous PT and root-hair bu

85 moving PP2CA, a negative modulator, the pir1 knockout mutant displayed an ABA-hyposensitive phenotype

86 Consistently with this observation, an npr3 knockout mutant displayed enhanced resistance to Pseudom

87 ibuted to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced susce

88 A Pseudomonas aeruginosa PPHD knockout mutant displays impaired growth in the presence

89 These knockout mutants document the importance of His biosynth

90 wer autophagosomes are generated in the NAP1 knockout mutant during starvation stress.

91 ALA10-knockout mutants exhibit reduced phospholipid uptake at

92 xpressing plants stayed green and the sgr2-1 knockout mutant exhibited early leaf yellowing under age

93 The DdTPS8 knockout mutant exhibited slow progression in developmen

94 not observed in slice cultures from Munc13-1 knockout mutants exhibiting defective vesicle priming.

95 We further show that a pgp4 knockout mutant exhibits an in vitro phenotype similar t

96 The s3h knockout mutants fail to produce 2,3-DHBA sugar conjugat

97 ispensable for seed development, as a double knockout mutant failed to make viable seeds.

98 ion of eggs that hatched from trpm germ-line knockout mutant females, although eggs were able to comp

99 A knockout mutant for Arabidopsis ADF4 has longer hypocoty

100 Physiological analysis of a transferred DNA knockout mutant for AtALMT1 as well as electrophysiologi

101 A knockout mutant for KNL2 shows a reduced level of cenH3

102 Finally, knockout mutants for 41 genes belonging to the different

103 This indicates the possible usage of TTM2 knockout mutants for agricultural applications to genera

104 We isolated gene knockout mutants for all 13 class XI myosins present in

105 Knockout mutants for athb13 showed increased primary roo

106 Knockout mutants for BGLU45 or BGLU46 do not have a lign

107 Creation of knockout mutants for Cel48S (also known as CelS, S(S), a

108 ) mutants, generated by crossing CRISPR/Cas9 knockout mutants for each gene, accumulated high levels

109 o, we conducted a comparative study of T-DNA knockout mutants for each Tic gene, and for the most abu

110 Knockout mutants for genes encoding either violaxanthin

111 Knockout mutants for putative BH4 synthetic enzyme genes

112 of Arabidopsis, including single and double knockout mutants for the high-affinity transporter, AtHA

113 d cytokinin degradation as well as different knockout mutants for the negative AUX/IAA regulators shy

114 lished a comprehensive study covering 269 TF knockout mutants for the yeast Saccharomyces cerevisiae.

115 of lignified tissues of various Arabidopsis knockout mutants (for AtCAD5, 6, and 9) at different sta

116 Unlike the wild type, an asr1734 knockout mutant formed 5% heterocysts after a nitrogen s

117 AOX1D expression is increased in aox1a knockout mutants from Arabidopsis (especially after rest

118 In nonimmune blood, fHBP knockout mutants from high-expressing stains do not surv

119 CML38-knockout mutants generated via T-DNA insertion were inse

120 relA, csdA, and dac2 knockout mutants grew more slowly at low temperature, bu

121 Knockout mutants grew poorly, but attenuation of Zn(II)2

122 Moreover, multiple sac knockout mutants had an increased number of smaller stor

123 fzl knockout mutants have abnormalities in chloroplast and t

124 Remarkably, slp1 knockout mutants have reduced protein and activity level

125 The T-DNA knockout mutant hcf222-2 showed a more severe defect wit

126 fer is specific to the donor strain; an lpqM knockout mutant in the recipient is still proficient in

127 Unlike the available set of homoplasmic knockout mutants in 25 plastid genes, the rbcL deletion

128 The system includes knockout mutants in all ABC and putative auxin transport

129 Analysis of alix knockout mutants in Arabidopsis showed that ALIX is esse

130 opy and that has not been reported with Mlh3 knockout mutants in Arabidopsis.

131 difficult to obtain homozygous or biallelic knockout mutants in citrus.

132 However, it has been shown that knockout mutants in fact reach the steady states with th

133 To address this, we generated knockout mutants in the moss Physcomitrella patens using

134 -Bar_gosGFP to produce activation-tagged and knockout mutants in the processing tomato cultivar M82.

135 lmonella enterica serovar Typhimurium double-knockout mutants in which either the lipoprotein A (lppA

136 y, we describe a method for rapidly creating knockout mutants in which we make use of yeast recombina

137 c program in Medicago truncatula TR25 (symrk knockout mutant) in the absence of rhizobia.

138 Analysis of knockout mutants indicated that GLR3.3 was a required co

139 Wall fractionation analysis of axy4 knockout mutants indicated that only a fraction containi

140 , the resulting nonfeasibility of creating a knockout mutant is a major limiting factor in studying i

141 However, an mfd knockout mutant is not hypersensitive to either aza-C-in

142 A triple cdkd knockout mutant is not viable, but a combination of null

143 wever, a striking characteristic of elt-2(0) knockout mutants is that while they die shortly after ha

144 rate that the backcrossing of wild mice with knockout mutant laboratory mice retrieves behavioural tr

145 An indh knockout mutant lacked complex I but had low levels of a

146 Knockout mutants lacking all EBs are viable and fertile

147 Targeted P. patens knockout mutants lacking either PpSMF1 or PpSCRM1 develo

148 and early seedling development, we utilized knockout mutants lacking one or more of these components

149 We show that a tpc1 knockout mutant lacks functional slow vacuolar channel a

150 Interestingly, a DdTPS8 knockout mutant lacks not only discoidol, but also a put

151 Here, we report that rsp knockout mutants lead to global transcriptional and prot

152 A T-DNA knockout mutant, lecrka4.1-1, slightly enhanced ABA inhi

153 Chloroplasts isolated from the atHsp93-V knockout mutant line are still able to import a variety

154 owth conditions, two independent Arabidopsis knockout mutant lines displayed significantly reduced le

155 tudy we isolated Arabidopsis T-DNA insertion knockout mutant lines for each of the genes encoding the

156 Several Arabidopsis knockout mutant lines for genes involved in polyester bi

157 Lipidomic analysis of knockout mutant lines of these five genes showed a signi

158 rotein import into chloroplasts, we isolated knockout mutant lines that contain T-DNA disruptions in

159 Using the corresponding knockout mutant lines, we show that bZIP17, bZIP60, BAG7

160 m, we generated two cyp71a12 cyp71a13 double knockout mutant lines.

161 N receptor (IFNLR1) and double IFNAR1/IFNLR1 knockout mutant mice had reduced lung inflammatory patho

162 Generation of knockout mutant mice has currently been achieved by many

163 Sperm of the double knockout mutant mice show responses to stimulus by HCO3

164 By using conditional knockout mutant mice, we report that neuronal autophagy

165 Smad1/5(CKO) (cartilage-specific knockout) mutant mice are nearly identical to Smad1/5(CK

166 We find homozygous Mllt10 knockout mutants (Mllt10-KO) exhibit midline facial clef

167 Seed of a knockout mutant, nic2-1, had reduced nicotinamidase acti

168 By examining AHA2 localization in a knockout mutant of a receptor protein kinase, FERONIA, w

169 A null T-DNA knockout mutant of AtAGP19 was obtained and compared to

170 uppressor screen of a transfer DNA insertion knockout mutant of AtCNGC2 in order to identify downstre

171 A knockout mutant of AtGPAT9 demonstrates both male and fe

172 In this report, we generated a knockout mutant of crgA (DeltacrgA) in the serum-sensiti

173 Prior infection with an fpv014-knockout mutant of FWPV still blocked transfected poly(I

174 Creation of a gene 5a knockout mutant of MHV-A59 demonstrated that a major com

175 These dynamics were largely absent in the knockout mutant of PSII core protein kinase SER/THR PROT

176 M NaCl treatment in wild-type rice (WT); GR3 knockout mutant of rice (gr3); and the functional gr3-co

177 lonized more efficiently on the sgc protease knockout mutant of S. gordonii than on the parent biofil

178 uced by transfection of a nonreplicative NS1 knockout mutant of the MVC infectious clone, as well as

179 An isogenic knockout mutant of the open reading frame NTHI1441 showe

180 to the identification of a grazer-resistant knockout mutant of the wzm ABC O-antigen transporter gen

181 Generation of a knockout mutant of this regulatory gene in the nonfood b

182 We constructed a knockout mutant of VC1758 (int) with V. cholerae strain

183 In either of the knockout mutants of AHL3 and AHL4, encoding closely rela

184 next isolated and characterized insertional knockout mutants of all three isoforms confirming a comp

185 Knockout mutants of Arabidopsis, deficient in various ke

186 Knockout mutants of AT5G41040 show almost complete elimi

187 Knockout mutants of At5g63560 were severely reduced in t

188 Knockout mutants of bHLH142 exhibited retarded meiosis a

189 The knockout mutants of CEK2 and CEK3 both affected root gro

190 Knockout mutants of CPRabA5e displayed delayed seed germ

191 e isethionate but not taurine; corresponding knockout mutants of D. alaskensis G20 did not grow with

192 , pollen fitness, and pollen tube growth for knockout mutants of five of the six myosin XI genes expr

193 Knockout mutants of genes for protease subunits are of l

194 Knockout mutants of GGT2 and GGT4 showed no phenotype.

195 e analyses of overexpressing hairy roots and knockout mutants of MtMYB5 and MtMYB14 indicate that MtM

196 Knockout mutants of Plasmodium falciparum lacking pfpm1,

197 o indicated by frequently subdued effects of knockout mutants of regulators, their evolutionary losse

198 The knockout mutants of svb showed enhanced tolerance to ER

199 In this study, we attempted to generate knockout mutants of the ortholog of yeast HOG1 MAP kinas

200 the gene was done by generating CRISPR/Cas9 knockout mutants of the orthologous tomato gene resultin

201 Three additional knockout mutants of the other components of the BCKDH co

202 stigating single, double and triple isogenic knockout mutants of the PhiHKU.vir-encoded exotoxins, we

203 Previous work has established that the knockout mutants of these genes display heat-sensitive p

204 Knockout mutants of this transporter failed to grow on m

205 We further tested the ATP-induced genes in knockout mutants of transcription factors, demonstrating

206 sition system to generate single- and double-knockout mutants of two tandemly duplicated cytochrome P

207 Knockout mutants of yajQ do not support the replication

208 ve heat shock response of mycobacterial sigE knockout mutants only in the presence of a functional my

209 cagA knockout mutants or CagA phosphorylation-defective mutan

210 In Arabidopsis, the oxoprolinase knockout mutants (oxp1-1 and oxp1-2) accumulate more 5-o

211 TF knockout mutant phenotypes are consistent with model pre

212 We further demonstrated that two knockout mutants (pilB and pilQ mutants) that are defici

213 AtTTM2 knockout mutant plants exhibit an enhanced hypersensitiv

214 The size of AtHIPM knockout mutant plants of Arabidopsis was slightly large

215 s (Arabidopsis thaliana) and phospholipase D knockout mutants pld zeta1, pld zeta2, and pld zeta1 pld

216 n FH domains 6 and 7 fused to Fc than double knockout mutants prepared from two sensitive meningococc

217 One knockout mutant produced fruiting bodies of abnormal sha

218 Knockout mutants produced more mycelium, particularly at

219 on is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpr

220 Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC-MS-b

221 in mice of 2,578 barcoded Plasmodium berghei knockout mutants, representing >50% of the genome, and c

222 Expression of CBP in the pme3 knockout mutant revealed that PME3 is required but not t

223 ariants produced by a M. tuberculosis Rv2181 knockout mutant revealed the presence of but a single Ma

224 ual module knockout mutant and single module knockout mutants revealed that 4-nitrotryptophan is an i

225 A novel, conditional FUS knockout mutant reveals that postnatal elimination of FU

226 nerated the recombinant rGLS virus and an NS knockout mutant, rGLSNSdelta virus, using reverse geneti

227 The knockout mutant sdg8 displays a reduced growth phenotype

228 lowed Arabidopsis wild type but not AtHSP101 knockout mutant seedlings to survive otherwise lethal te

229 s transposition in calli derived from dng701 knockout mutant seeds compared with that in wild-type ca

230 Rice cslf6 knockout mutants show a slight decrease in height and st

231 ted by the activated epicardium and an nrp1a-knockout mutant showed a significant delay in heart rege

232 A mot1.3-1 knockout mutant showed impaired growth concomitant with

233 Interestingly, an sigH-knockout mutant showed increased sensitivity to a sustai

234 An oppA knockout mutant showed marked impairment in its capacity

235 The znu knockout mutant showed marked impairment in its capacity

236 In addition the NarK2 knockout mutant showed no defect in nitrite export.

237 susceptibility to H. schachtii, while a pme3 knockout mutant showed opposite phenotypes.

238 in different organs of wild-type and a ggt3 knockout mutant showed that GGT3 was a major contributor

239 Analysis of LDIP T-DNA knockdown and knockout mutants showed a decrease in LD abundance and a

240 lysaccharide preparations from RGP1 and RGP2 knockout mutants showed a significant reduction in total

241 Knockout mutants showed impaired stomatal closure in res

242 arization, while CRISPR-Cas9-mediated single knockout mutants showed precocious endosperm cellulariza

243 ESCRT knockout mutants showed that the release enhancement was

244 AKR2A knockdown and kcs1 knockout mutants showed the worst performance under chil

245 An fcbT1T2T3 knockout mutant shows a much slower growth rate on 4-chl

246 ation across experimental conditions for all knockout mutants simultaneously.

247 ota/1145/2007 (H3N2) (SIV 1145-WT), a PB1-F2 knockout mutant (SIV 1145-KO), and its N66S variant (SIV

248 The quadruple knockout mutants still contained about 10% of the wild-t

249 aracterization of wild-type P patens and the knockout mutant stn8 (depleted in SER/THR PROTEIN KINASE

250 Plate-killing assays indicate that a PE-knockout mutant strain of Escherichia coli drastically o

251 n of the growth of the Escherichia coli uxaB knockout mutant strain on galacturonate.

252 The resulting putA knockout mutant strain was characterized by oxidative st

253 periodontal lesions, both capsular-defective knockout mutant strains of P. gingivalis induced less al

254 Arabidopsis hpat1/2/3 triple knockout mutants suffer from a strong male sterility def

255 RNA blots and analysis of enzyme activity in knockout mutants suggest that GGT1 is expressed most str

256 A triple-knockout mutant tga2-1 tga5-1 tga6-1 was shown previousl

257 )) of neuronal SLO-2 is ~50% lower in scyl-1 knockout mutant than wild type.

258 We have now constructed an E knockout mutant that confirms that the highly defective

259 The acs1 knockout mutant that has a disruption in the plastidic a

260 and actin-interacting protein 1, as well as knockout mutants that lack Coronin and actin-interacting

261 In an fra1-5 knockout mutant, the expansion rate of the inflorescence

262 We used a fliL knockout mutant to gain further insight into the functio

263 Failure of an E. ictaluri urease knockout mutant to increase the ECV pH in the in vivo ra

264 ted the relative lethality of 11 known mouse knockout mutants to categorize essentiality.

265 Here, we used overexpression lines and knockout mutants to examine the role of SPL10 in floweri

266 The different phenotypes of Phox2 knockout mutants, together with their asynchronous onset

267 The increased sensitivity of atpollambda knockout mutants toward high salinity and MMC treatments

268 egulation by testing overexpression (OE) and knockout mutants under heat stress.

269 ative analysis of seedling growth of snrk2.4 knockout mutants versus wild-type Arabidopsis suggests t

270 ally induced apoptosis indicated that the NS knockout mutant virus induces earlier and increased DNA

271 Compared to the wild-type virus, the ToV-PLP knockout mutant virus showed impaired growth and induced

272 tosis indicated that the NV-deficient and NV knockout mutant viruses induce apoptosis earlier in cell

273 ed earlier, amylovoran production in an amyR knockout mutant was about eight-fold higher than that in

274 t Dicarboxylate Transporter (tDT) in the tdt knockout mutant was associated previously with an impair

275 The pgk3.2 knockout mutant was characterized by reduced growth but

276 d as a lipoprotein and purified, and an oppA knockout mutant was constructed.

277 The lpp double-knockout mutant was defective in invading and inducing c

278 To study its physiological role, a SynK-less knockout mutant was generated and characterized.

279 role of LytSR in biofilm development, a lytS knockout mutant was generated from a clinical S. aureus

280 In addition, the rgga knockout mutant was hypersensitive to ABA in root growth

281 t in reduced virulence; however, an mltE(EA) knockout mutant was reduced in virulence and growth in i

282 The Crvtc2-1 knockout mutant was viable and, depending on the growth

283 rimary roots in PLDzeta1 and PLDzeta2 double knockout mutants was slower than that of wild type and s

284 y comparative metabolomics of overexpression/knockout mutants, we identified a tryptophan-derived iro

285 ugh construction and analysis of a series of knockout mutants, we identified the genes necessary for

286 Moreover, while the ccm knockout mutants were completely incompetent for photosy

287 dadRAX knockout mutants were constructed and subjected to analy

288 Two constitutive double knockout mutants were generated (designated as dpe2-1xph

289 B'theta knockout mutants were impaired in peroxisomal beta-oxida

290 Strikingly, the pdx1 knockout mutants were impaired in root growth and early

291 MAM overproduction and knockout mutants were more and less mitogenic, respectiv

292 The knockout mutants were more efficiently ingested and kill

293 lated in As-treated Arabidopsis, and ggct2;1 knockout mutants were more tolerant to As and cadmium th

294 ssing the ppi3 mutants with ppi1, an atToc33 knockout mutant) were unsuccessful, indicating that the

295 In contrast, an isogenic fHbp knockout mutant, which grew well in broth, was rapidly k

296 wo independent pal1 pal2 pal3 pal4 quadruple knockout mutants, which are stunted and sterile.

297 h the investigation of independent complex I knockout mutants, which were found to have corresponding

298 e markedly opposite phenotypes to the double knockout mutant, with increased cell-wall methylesterifi

299 t created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants).

300 tophore type, we engineered asip1 homozygous knockout mutant zebrafish.