ライフサイエンスコーパス: lac repressor

1 DNA-binding protein making stable DNA loops (lac repressor).

2 uences by fusing each of the chimeras to the Lac repressor.

3 tor by affecting the binding affinity of the lac repressor.

4 romosomes were isolated using an immobilized lac repressor.

5 phosphates and positively charged groups on lac repressor.

6 teraction on ligand binding and allostery in lac repressor.

7 the RNA pol I termination factor, Reb1p, or lac repressor.

8 ed forms of V52C was comparable to wild-type lac repressor.

9 ts gene that encodes a temperature-sensitive lac repressor.

10 sfection and served as a tag for labeling by lac repressor.

11 y than similar libraries based on the intact lac repressor.

12 e cat expression in rat cells expressing the lac repressor.

13 f DNA can be constrained in a stable loop by Lac repressor.

14 ional states of the dimeric Escherichia coli Lac repressor.

15 c operator sequences and mixed them with the lac repressor.

16 L gene was regulated by the Escherichia coli lac repressor.

17 tringently regulated by the Escherichia coli lac repressor.

18 riments on some model systems, primarily the Lac repressor.

19 the monomer-monomer subunit interface in the lac repressor, a mutation that generates dimeric protein

20 Its key component is the lac repressor, a product of the lacI gene.

21 e individual roles played by H74 and D278 in lac repressor allostery appear more important than their

22 atch substrates containing an end blocked by lac repressor allowed us to identify MSH proteins presen

23 addition of a ligand that weakens binding by Lac repressor also induced lysogens.

24 liding toward the LacO site, the presence of Lac repressor also stimulated sliding in the opposite di

25 replacing lambda Cro with a dimeric form of Lac repressor and adding several lac operators.

26 resembling the sugar-binding portion of the lac repressor and another with similarity to RNase H.

27 -tail orientations, as well as EcoRI(E111Q), lac repressor and even nucleosomes.

28 the hybrid tac promoters is repressed by the lac repressor and induced by the non-metabolizable subst

29 had such a strong impact on a field, as the lac repressor and lambda repressor have had in Molecular

30 with data sets of unbiased mutations in the lac repressor and lysoyzyme.

31 , which inhibits the interaction between the lac repressor and operator).

32 the well-characterized DNA-looping proteins Lac repressor and phage lambda CI to measure interaction

33 laced lambda Cro with a module that included Lac repressor and several lac operators.

34 VP16 as well as nucleosome positioning by R3 Lac repressor and subsequent nucleosome remobilization u

35 e extensive mutation data from two proteins, Lac repressor and subtilisin, to perform such an analysi

36 A hybrid protein (H144), consisting of Lac repressor and T7 endonuclease I, binds at the lac op

37 xpression of the PAB1 gene to control by the lac repressor, and by linking lac repressor expression t

38 latory system (ARES), based on the bacterial lac repressor, and demonstrate its utility for controlli

39 he core and the second DNA-binding domain of lac repressor, and result in a complex which is looped b

40 Here, we use Lac repressor as a tool to probe how a DNA-bound factor

41 on the C-terminal tetramerization domain of Lac repressor, as a model system for four-chain coiled c

42 d by a simple competition formalism in which lac repressor behaves a a specific-binding oligocation (

43 repression of transcription, whereas Fis and Lac repressor binding is not osmotically sensitive.

44 ulovirus early promoter containing optimized Lac repressor binding sites (lac operators).

45 teraction in which CAP bends the DNA and the lac repressor binds simultaneously to its operator site

46 se utilization are negatively regulated; the lac repressor binds to an upstream operator blocking the

47 ned the X-ray crystal structure of a dimeric Lac repressor bound to a 22 base-pair DNA with the natur

48 Previous structures of Lac repressor bound to DNA used a fully symmetric "ideal

49 The crystal structures of the lac repressor bound to inducer and anti-inducer molecule

50 structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-

51 ble lysogens in the presence of a ligand for Lac repressor but switched to the lytic state when the l

52 bility of the isolated coiled-coil domain of Lac repressor by testing the effect of mutations at this

53 alactopyranoside (IPTG), an inhibitor of the lac repressor, can prevent demethylation of the lac oper

54 xplore specific sequence determinants of the lac repressor coiled-coil tetramerization domain, we hav

55 opyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric op

56 Contributions to the local Lac repressor concentration come from free repressor and

57 non-supercoiled plasmid was used to express lac repressor constitutively while a circular plasmid wa

58 at contained the human C/EBPalpha gene under Lac repressor control.

59 wrapping proteins including the nucleosome, lac repressor core tetramer, RNA polymerase core protein

60 nd to prevent it from repressing cI, because lac repressor could not repress P(RM) in our constructs.

61 monstrated that the dimer-dimer interface of lac repressor could persist at higher levels of urea tha

62 iding RNA polymerases blocked by a DNA-bound lac repressor could slowly re-initiate transcription and

63 preferentially shifts nucleosomes away from Lac repressor, demonstrating that a DNA-bound factor def

64 Addition of Lac repressor directly demonstrates that bound repressor

65 tional cyan fluorescent protein (CFP)-tagged lac repressor-ER chimera (CFP-LacER) was used in live ce

66 ac operator sites either directly, through a lac repressor-ER fusion protein (lac rep-ER), or indirec

67 be fine-tuned in a supervised fashion using lac repressor experimental datasets to more effectively

68 troduction into the transformed cells of the lac repressor, expressed from a second retroviral vector

69 control by the lac repressor, and by linking lac repressor expression to the disease resistance state

70 Transcription factors (TFs) such as the lac repressor find their target sequence on DNA at remar

71 to involve competition of RNA polymerase and Lac repressor for overlapping binding sites.

72 tion of the hinge helices and release of the Lac repressor from the operator.

73 c-operator repeat in cells stably expressing lac repressor fused with enhanced green fluorescent prot

74 binding of a green fluorescent protein (GFP)-Lac repressor fusion expressed from the HIS3 promoter.

75 A VP16-lac repressor fusion protein targeted the VP16 acidic ac

76 in vivo assay in which UBF is targeted via a lac repressor fusion protein to a heterochromatic, ampli

77 In this paper, we show that a LacI (Lac repressor) fusion of HJURP drove the stable recruitm

78 equence-specific DNA-binding proteins; i.e., lac repressor, gal repressor, and lambda O protein, are

79 a 7-kb fragment consisting of a venus-tagged lac repressor gene along with a target lacZ reporter int

80 ty of the method by separately inserting the lac repressor gene and the lacZ target into the chromoso

81 We provide evidence from Escherichia coli Lac repressor gene regulatory loops supporting these hyp

82 the in vitro looping dynamics of the classic Lac repressor gene-regulatory motif.

83 loci on the right arm of chromosome IV and a Lac repressor-GFP fusion protein, we were able to visual

84 st cells and made visible by expression of a lac repressor-green fluorescent fusion protein.

85 Because the lac repressor homotetramer contains two DNA binding modu

86 Production of a GFP-Lac repressor hybrid protein in cells carrying F or P1 p

87 or binding sites, and a repressor census for Lac repressor in Escherichia coli.

88 ions of mouse and human cells expressing the lac repressor, in parallel with the constitutively expre

89 CAP-lac repressor interactions may play important roles in r

90 DNA looping mediated by the Lac repressor is an archetypal test case for modeling pr

91 DNA binding by the Escherichia coli lac repressor is mediated by the approximately 60 amino

92 y quickly; the experimental binding rate for lac repressor is orders-of-magnitude higher than predict

93 The lactose (lac) repressor is an allosteric protein that can respond

94 , we directly observed specific binding of a lac repressor, labeled with a fluorescent protein, to a

95 of the monomeric DNA binding domain (DBD) of lac repressor (lac DBD) and on formation of an oligomeri

96 The large stabilizing effect of GB on lac repressor-lac operator binding is predicted quantita

97 putrescine concentration on a specific PNAI (lac repressor-lac operator) as a function of inverted qu

98 expressed inducibly in human cells using the lac repressor/lac operator regulatable promoter system.

99 The Lac repressor/lac operator system was used to label spec

100 m) on the formation of 1:1 complexes between lac repressor (LacI) and its symmetric operator site (Sy

101 Lac repressor (LacI) binds two operator DNA sites, loopi

102 ce indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational

103 Lac repressor (LacI) forms DNA loops which are critical

104 eir 5'-flanking sequence with or without the lac repressor (lacI) gene, conditionally expressed tRNAs

105 Using a coarse-grained model of DNA and lac repressor (LacI) in the Escherichia coli nucleoid, s

106 The interaction of lac operator DNA with lac repressor (LacI) is a classic example of a genetic r

107 The lac repressor (LacI) is a well characterized transcripti

108 anding gene control by DNA looping where the lac repressor (LacI) protein competes with RNA polymeras

109 e chromosomal protein 6A (Nhp6A), facilitate lac repressor (LacI) repression loops in E. coli.

110 was developed using the lac operator (lacO)/lac repressor (LacI) system from Escherichia coli.

111 ese questions we investigated the binding of lac repressor (LacI) to a series of 40 bp fragments carr

112 Now, Poelwijk et al. report evolving the lac repressor (LacI) to reverse its regulatory logic, re

113 The rates of association of the tetrameric Lac repressor (LacI), dimeric LacIadi (a deletion mutant

114 ligand-responsive transcription factor, the lac repressor (LacI), in different functional states (ap

115 Some proteins, like the lac repressor (LacI), mediate long-range loops that alte

116 A known DNA loop-forming protein, the lac repressor (LacI), was used to confirm that cooperati

117 of insertion mutants of the Escherichia coli lac repressor (LacI).

118 We engineered an aTF, the Escherichia coli lac repressor, LacI, to respond to one of four new induc

119 ontrol of the lac promoter by the regulators Lac repressor (LacR) and cAMP-receptor protein (CRP).

120 on of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous

121 (lacO) sequences is first bound to purified lac repressor (LacR).

122 t was 170-fold stronger, consistent with the lac repressor levels required to control LacI-regulated

123 For example, the probability of lac repressor-mediated looping in individual molecules r

124 We have introduced sequences encoding the lac repressor of Escherichia coli into the genome of the

125 t transcriptional repression mediated by the lac repressor of Escherichia coli using experimental mea

126 us purine holorepressor-operator complex and lac repressor-operator complex.

127 Using Lac repressor-operator complexes as roadblocks, we show

128 Here we show that as few as three lac repressor-operator complexes block Escherichia coli

129 forks reconstituted in vitro and blocked by lac repressor-operator complexes.

130 The Lac repressor-operator interaction was used as a reversi

131 We have utilized the Escherichia coli lac repressor-operator system to test whether protein bi

132 we used an in vivo microscopy assay based on Lac Repressor/operator recognition to show that Mcp, a p

133 we utilize the well-defined Escherichia coli lac repressor/operator system in human cells to determin

134 ere, using the well defined Escherichia coli lac repressor/operator system, we have found that direct

135 We have constructed a lac repressor/operator-based system to tightly regulate

136 n splits using 5,009 single mutations of the lac repressor, our best-performing model achieved a medi

137 mponent of lambda's genetic switch, with the lac repressor (plus two lac operators).

138 se results suggest that, in the areas of the lac repressor probed by these substitutions, the inducer

139 substitution, K84L, in the Escherichia coli lac repressor produces a protein that has substantially

140 ltiscale simulation of a complex between the lac repressor protein (LacI) and a 107-bp-long DNA segme

141 The lac repressor protein (LacI) efficiently represses trans

142 e by the binding of constitutively expressed Lac repressor protein (LacI) to operator sequences withi

143 ives that constitutively express the E. coli Lac repressor protein (LacI).

144 yeast, use of the green fluorescent protein-lac repressor protein allowed extended, in vivo observat

145 Chromosome tagging with GFP-Lac repressor protein allowed us to track, for the first

146 ctor-containing segments were enriched using lac repressor protein and then self-ligated.

147 Lac repressor protein binds simultaneously to two operat

148 ces hindered, RNA polymerase passage through lac repressor protein bound to natural binding sites.

149 eported on four single-tryptophan mutants of lac repressor protein from Escherichia coli: H74W/Wless,

150 theless, the physical mechanism by which the Lac repressor protein prevents transcription of the lact

151 were fused with a modified Escherichia coli Lac repressor protein that contains a nuclear localizati

152 A key element in the ability of lac repressor protein to control transcription reversibl

153 , because when translocation is blocked by a Lac repressor protein, DNA cleavage is similarly blocked

154 he lac operator sequence, which binds to the lac repressor protein, providing a simple and rapid way

155 In the inducer-bound structure of the lac repressor protein, the side chains of H74 and D278 a

156 n DNA duplexes and a DNA duplex bound to the Lac repressor protein.

157 ids into erythroid K562 cells expressing the lac repressor protein.

158 system based on the interaction between the lac repressor (protein) and operator (DNA) and its inter

159 Direct control of mammalian promoters by the lac repressor provides tight, reversible regulation, pre

160 Here we characterize TS for lac repressor(R)-lac operator(O) binding by analyzing ef

161 The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base

162 l interfaces and unfolding of the tetrameric lac repressor, reflecting the exceptional stability of t

163 We have developed lac repressor-regulated retroviral expression vectors th

164 tribution of nucleosomes away from the bound Lac repressor site.

165 In searching for the operator, a lac repressor spends approximately 90% of time nonspecif

166 ep-ER but not by wild-type ER recruited by a lac repressor-SRC570-780 fusion protein.

167 Lac repressor staining provided high resolution labeling

168 onal and professional journey, starting from Lac repressor studies, progressing to studying bacterial

169 red TALED repression system with the natural lac repressor system.

170 me under the control of the Escherichia coli lac repressor system.

171 g of these enhanced cyan fluorescent protein-lac repressor-tagged RARalpha-containing proteins to the

172 In our studies of lac repressor tetramer (T)-lac operator (O) interactions

173 nvolves cooperative binding of the bidentate lac repressor tetramer to pairs of lac operators via DNA

174 The Lac repressor tetramerization domain forms an antiparall

175 esigned peptides are based on the 20-residue Lac repressor tetramerization domain.

176 f Saccharomyces cerevisiae fused to a mutant lac-repressor that binds its operator with increased aff

177 e three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous

178 -induced ensemble reweighting in the E. coli lac repressor to describe the functional switching mecha

179 ncreatic ribonuclease A and Escherichia coli lac repressor to double-stranded DNA immobilized on cell

180 ers to serve as pairing sites and a modified lac repressor to perform the role of a protein cross-bri

181 nding of wild-type Escherichia coli lactose (lac) repressor to its regulatory site (operator O1) in t

182 The key component is a lac repressor transgene that resembles a typical mammali

183 we have determined the methylation status of lac repressor transgenes encoded by either the bacterial

184 ntiparallel four-stranded coiled coil of the lac repressor type in which the a, d, and e side chains

185 itution of Cys for Val at position 52 of the lac repressor was designed to permit disulfide bond form

186 e T7 RNA polymerase and the Escherichia coli lac repressor was inserted into an alternative site in t

187 Tethering in vitro with the Lac repressor was measured for the same 600-to 3,200-bp

188 This finding indicates that Lac repressor was present in the lysogens and was necess

189 Binding by the enhanced lac repressor was sufficiently tight to allow strong att

190 nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (A

191 Genomic analyses indicated that lac repressors were co-selected only within the conserve

192 hese lac operator-tagged spots, stained with lac repressor, were measured in isolated metaphase chrom

193 uorescent protein (GFP) fusions to LacI, the lac repressor, which binds to lacO-related sequences in

194 ween green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator b

195 xpression of a tetramerizing form of the GFP-Lac repressor, which can bind Lac operators on two diffe

196 elute C/EBP, more salt was required to elute lac repressor, while Gal4 showed a biphasic dependency w

197 in the intact phage with a module including Lac repressor, whose function is tunable with small mole

198 otein (lac rep-ER), or indirectly, by fusing lac repressor with the ER interaction domain of the coac

199 use was controlled by the interaction of the lac repressor with the regulatable Tyrosinase transgene