ライフサイエンスコーパス: lacZ gene

1 ing the Escherichia coli beta-galactosidase [LacZ] gene).

2 to indicator mice containing a cre-activated LacZ gene.

3 utant (hrR3) expressing the Escherichia coli lacZ gene.

4 n and frameshift mutations that occur in the lacZ gene.

5 , the green fluorescent protein gene, or the lacZ gene.

6 /no tail genes with dsRNA from the bacterial lacZ gene.

7 in the alpha-complementation fragment of the lacZ gene.

8 sed to the rrn boxA sequence followed by the lacZ gene.

9 more, there was no loss of expression of the lacZ gene.

10 promoter coupled to the coding region of the LacZ gene.

11 a fusion of this regulatory region with the lacZ gene.

12 to drive expression of the Escherichia coli lacZ gene.

13 isolated and linked to the Escherichia coli lacZ gene.

14 ta-galactosidase, the protein product of the lacZ gene.

15 1/scip/oct-6 gene has been replaced with the LacZ gene.

16 egulatory sequences and the Escherichia coli lacZ gene.

17 e coding sequences with the Escherichia coli lacZ gene.

18 oding region fused in frame to the bacterial lacZ gene.

19 y replacing common exons 8, 9, and 10 with a lacZ gene.

20 d in the trpR gene and coding resumed in the lacZ gene.

21 4+ cells that did not show expression of the lacZ gene.

22 It is also not active on a transfected lacZ gene.

23 Satb2 locus was disrupted by insertion of a LacZ gene.

24 required for maximum expression of the psn::lacZ gene.

25 g all of the coding exons with the bacterial lacZ gene.

26 control strains and a strain expressing the LacZ gene.

27 using a transposon containing a promoterless lacZ gene.

28 g part of the Sax2 coding sequences with the lacZ gene.

29 dramatically reduced cleavage of the mutated lacZ gene.

30 sing gene targeting to replace exon 6 with a lacZ gene.

31 ated Adamts7(-/-) mice carrying a knocked-in LacZ gene.

32 ameshifting or stop codon readthrough of the lacZ gene.

33 f amino acid residues at position 461 in the lacZ gene.

34 re a therapeutic gene is substituted for the lacZ gene.

35 cts of N-terminal p53 and N-terminal deleted LacZ genes.

36 erichia coli tryptophan repressor, trpR, and lacZ genes.

37 ing the Escherichia coli beta-galactosidase (lacZ) gene.

38 131I-labeled FIAU % dose/g) and coexpressed lacZ gene activity.

39 or carrying the cytomegalovirus (CMV)-driven LacZ gene (Ad-CMV-LacZ).

40 astin promoter was inserted either 5' to the LacZ gene (Ad-Lp-LacZ) or 5' to the cytosine deaminase (

41 RSVIL-1ra, control adenovirus containing the lacZ gene (Ad.RSVlacZ), or saline was injected into the

42 similar vector carrying the Escherichia coli lacZ gene (Adbetagal).

43 cted with Ad expressing the Escherichia coli LacZ gene (AdLacZ).

44 an alpha-chymase, coupled to the prokaryotic lacZ gene allows the targeting of beta-galactosidase to

45 is switch system was initially tested in the lacZ gene and a 600-fold induction of beta-galactosidase

46 into a synthetic intron between the reporter lacZ gene and either the LAT or the HSV-1 thymidine kina

47 transduction with a retrovirus encoding the lacZ gene and grafted to athymic mice.

48 TCC 7962 is located 3 kb downstream from the lacZ gene and is transcribed in the opposite orientation

49 ranscriptional fusion between a promoterless lacZ gene and the fimA promoter region was constructed.

50 ne promoters to express the Escherichia coli lacZ gene and the human alpha-globin gene from either ep

51 promoter region was fused to a promoterless lacZ gene and transformed into M. gallisepticum by using

52 hin a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by

53 igated during development using an inducible lacZ gene as an in vivo probe.

54 s used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of

55 Using lacZ genes as portable regions of homology for inversion

56 on transcriptionally fused to a promoterless lacZ gene at a nonessential, ectopic locus.

57 logous recombination to insert the bacterial lacZ gene between the transcription and translation init

58 Excision of the LacZ gene by Cre-mediated recombination initiates expres

59 AAV vector carrying the Cre recombinase, the lacZ gene can be activated and remain active even when r

60 nt and spatial relationships of HSV-1-tk and lacZ gene coexpression in culture and in vivo indicate t

61 vector replication, spread and HSV-1-tk and lacZ gene coexpression in vivo, first- or second-generat

62 For the assessment of HSV-1-tk and lacZ gene coexpression, 0.2 mCi of 131I-labeled FIAU was

63 ing higher expression efficiency of the tnaA-lacZ gene construct than the UAG stop codon.

64 enerated transgenic mice with N-CAM promoter/lacZ gene constructs containing either a wild-type or a

65 transgenic mouse lines carrying chimeric EPO-lacZ gene constructs.

66 iral vectors were used to introduce a mutant lacZ gene containing an I-SceI cleavage site and to effi

67 t beta-galactosidase activity in vivo from a lacZ gene containing an in-frame TGA codon.

68 Ns promote DNA homologous recombination of a LacZ gene containing paired AvrXa7 or asymmetric AvrXa7/

69 or vein grafts in transgenic mice expressing LacZ gene controlled by TIE2-endothelial specific gene p

70 served in the transgenic mice expressing the lacZ gene downstream from the Flt-1 promoter.

71 ls were crossed with mice transgenic for the LacZ gene downstream of a cassette of tet operator (TRE)

72 A construct containing the bacterial LacZ gene driven by a fragment of the beta-PDE 5' flanki

73 ng at the same genetic locus in MEL cells, a LacZ gene driven by the human beta-globin promoter linke

74 otein E (apoE)(-/-)/transgenic mice carrying LacZ genes driven by an endothelial TIE2 promoter.

75 n vein grafts using transgenic mice carrying LacZ genes driven by an endothelial TIE2 promoter.

76 rize the iCre developmental pattern with the LacZ gene encoding beta-galactosidase enzyme activity as

77 The lacZ gene encoding beta-galactosidase historically has b

78 n knock-in mice, Msx2 gene was replaced by n-LacZ gene encoding beta-galactosidase.

79 oactivity (assessed by autoradiography), and lacZ gene expression (assessed by beta-galactosidase sta

80 r cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off s

81 , a linear relationship was observed between lacZ gene expression and FIAU accumulation 5-40 h after

82 e have designed a 19F NMR approach to reveal lacZ gene expression by assessing beta-galactosidase (be

83 lays induction of embryonic DeltaSmadnkx-2.5/lacZ gene expression during early heart formation.

84 tyrosine hydroxylase (TH) promoter to drive lacZ gene expression elicits the transduction of the hig

85 A rim of lacZ gene expression immediately adjacent to necrotic tu

86 idual clones were screened for activation of lacZ gene expression in cardiac myocytes developing in v

87 inistration of AdSM22-lacZ did not result in lacZ gene expression in the liver or lungs.

88 d 13 appears more advantageous for assessing lacZ gene expression in vivo.

89 pha-responsive reporter constructs, in which lacZ gene expression is driven by minimal Hsp70 gene pro

90 LacZ gene expression of the protein beta-gal was demonst

91 -beta-D-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color se

92 Quantitative analysis of reporter lacZ gene expression showed that the viral vector-mediat

93 LacZ gene expression was observed up to 3 months after t

94 When tetracycline was withdrawn, ICE-lacZ gene expression was rapidly turned on and apoptosis

95 Here, we found that lacZ gene expression was repressed in aerated cultures o

96 of the green fluorescent protein, monitoring lacZ gene expression with a chromogenic reporter, mappin

97 ments conferred tracheal epithelial-specific LacZ gene expression, but parenchymal lung expression wa

98 in KB and CD34+ cells that were positive for lacZ gene expression, little activity was detected in M0

99 y 50% of the donors showed various levels of lacZ gene expression, the expression was undetectable in

100 mediated greater than 100-fold repression of lacZ gene expression, whereas the napF operon 7-2-7 hept

101 19F NMR-sensitive aglycons for detection of lacZ gene expression.

102 A26R mouse model or cell line that carries a lacZ gene flanked by two loxP sites.

103 onic complementation of the Escherichia coli lacZ gene for use in mammalian cells.

104 on restores the reading frame of a disrupted lacZ gene, forming blue papillae on lactose X-gal plates

105 er injection with an AAV vector containing a lacZ gene fragment, and precise correction of the 4-bp d

106 codon) was amplified by PCR, ligated with a lacZ gene from E. coli, and inserted into the Tn4001-con

107 Expression of the lacZ gene from these constructs was repressed to various

108 Expression of the lacZ gene from this construct is repressed by nitrate in

109 ene expression was monitored by using an icd-lacZ gene fusion and shown to vary over a 15-fold range

110 e investigated by using a set of three alc'-'lacZ gene fusion constructs that were contiguous with th

111 The expression of a rdgA-lacZ gene fusion in E. coli MC4100 is suppressed upon ov

112 ng beta-galactosidase production from a lasB-lacZ gene fusion in the presence of the transcriptional

113 Furthermore, the expression of an rsmA-lacZ gene fusion is lower in the RsmC(-) mutant than in

114 btilis strain in which expression of a vanH::lacZ gene fusion is regulated by VanR and VanS of Entero

115 and a deletion analysis using a lambda metE-lacZ gene fusion suggested that there is no specific cis

116 An aegA-lacZ gene fusion was constructed and examined in vivo; a

117 An rbsB-lacZ gene fusion was constructed and used to select muta

118 The expression of comS'::'lacZ gene fusion was reduced by the sinR mutation, but b

119 Using a dyn-1::lacZ gene fusion, a high level of dynamin expression was

120 Using this fragment to generate a carB::lacZ, gene fusion construct, we investigated carB expres

121 We then constructed R1 region-lacZ gene fusions and used the resulting R1 repeat-beta-

122 ene fusions extends the benefits of targeted lacZ gene fusions by providing the additional utility of

123 Previously, targeted lacZ gene fusions have been very useful for analyzing mo

124 s appear to increase expression of the tnp'-'lacZ gene fusions used in this screen, by increasing tra

125 ctivation, a series of transcriptional lasBp-lacZ gene fusions was constructed.

126 in the P. aeruginosa quorum-sensing cascade, lacZ gene fusions were used to determine the effect of P

127 d in R. capsulatus using ccdA:phoA and ccdA :lacZ gene fusions.

128 s also demonstrated using oppA-phoA and dppA-lacZ gene fusions.

129 pecifically induced by phthalate as shown by lacZ gene fusions.

130 well with results obtained using individual lacZ gene fusions.

131 nase activity and by expression of phi(adhE'-lacZ) gene fusions.

132 As assessed by the frequency of functional lacZ gene generation from vectors in which lacZ was disr

133 d minK-deficient mice in which the bacterial lacZ gene has been substituted for the minK coding regio

134 iophage DNA containing the lesion within the lacZ gene in 4 local sequence contexts.

135 Fusing this region to a lacZ gene in a promoter probe vector demonstrated that a

136 of inactivating mutations introduced in the lacZ gene in a single replication cycle provided a measu

137 Specific integration into the lacZ gene in E. coli was obscured by a high background o

138 e was inactivated by inserting the bacterial lacZ gene in frame after codon 131 of the gp64 efp gene.

139 ring the in vivo rate of inactivation of the lacZ gene in one replication cycle of SNV- and murine le

140 28) was required to obtain expression of the lacZ gene in osteoblasts, whereas the C sequence (-1575

141 consistent and high-level expression of the lacZ gene in osteoblasts.

142 a(1A)-AR knockout mouse, which expresses the LacZ gene in place of the coding region for the alpha(1A

143 actosidase expression in mice containing the lacZ gene in place of the Flk-1 gene are consistent with

144 to direct specific expression of the E. coli lacZ gene in transgenic mice, whereas a 2-kilobase pair

145 ter inserting a CSC-box into a plasmid-bound lacZ gene in Y. enterocolitica, the mRNA of this constru

146 grafted into a chimeric mouse carrying TIE2-LacZ genes in bone marrow cells, a proportion of cells d

147 ne was replaced by the beta-D-galactosidase (lacZ) gene in the germ line.

148 beta-galactosidase (which is encoded by the LacZ gene) in the locus coeruleus by twofold compared wi

149 frequencies of mutations of the virus-borne lacZ gene increased significantly in the substitution mu

150 ons of dotA to the Escherichia coli phoA and lacZ genes indicated that DotA is an integral cytoplasmi

151 le12]-alpha-factor in growth arrest and FUS1-lacZ gene induction assays.

152 el that contains a mutant, nuclear-localized lacZ gene inserted at the ubiquitously expressed ROSA26

153 he gamma(1)34.5 gene, R849, which contains a lacZ gene inserted in place of the gamma(1)34.5, R908, w

154 The lacZ gene inserted into the mafF locus revealed prominen

155 mice with the bacterial beta-galactosidase (lacZ) gene inserted downstream of the endogenous Ednrb p

156 648) or an insertion of the Escherichia coli lacZ gene into a truncated U(L)15 exon II [designated HS

157 Insertion of a promoterless lacZ gene into the chvD locus greatly attenuated virulen

158 utant virus was constructed by inserting the lacZ gene into the coding region of mtase1.

159 strate the stable insertion of the bacterial lacZ gene into the H. zea genome.

160 null mice were generated by insertion of the lacZ gene into the Nor-1 genomic locus.

161 uses with insertions of the Escherichia coli lacZ gene into the predicted gE and gI reading frames.

162 ector containing the beta-galactosidase (AAV-lacZ) gene into adult BALB/c mice, protein expression wa

163 e assay in vitro since the expression of the lacZ gene is dependent on M2-1.

164 utilized mice in which the Escherichia coli lacZ gene is integrated into the neurotrophin-3 locus (N

165 In this fashion, the lacZ gene is placed under the regulatory control of the

166 The bacterial lacZ gene is widely used as a reporter in a myriad of mo

167 ite selection based on the disruption of the lacZ gene, it is rarely practiced due to its high backgr

168 h the gC coding sequence was replaced by the lacZ gene (KCZ) was substantially more impaired (ca. 65%

169 for a Kit null allele marked by an inserted LacZ gene (Kit(W-LacZ)), we show that the onset of Mitf

170 nserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early

171 Eleven different BmNPV mutants carrying the lacZ gene marker at various distances (1.4 to 61.7 kb) f

172 in gene (polh) locus and a second carrying a lacZ gene marker cassette.

173 effusion lymphoma cell lines, activated the lacZ gene of T1H6 in a sensitive and dose-dependent mann

174 Mutant frequency in the lacZ gene of the pUR288 plasmid was determined in DNA is

175 s construct was crossed into the chromosomal lacZ gene of V. cholerae.

176 cause of a frameshift mutation affecting the lacZ gene on its episome.

177 hat is out of frame relative to a downstream lacZ gene on the chromosome to examine transcriptional s

178 vectors were used to transfer the bacterial lacZ gene or a synthetic green fluorescent protein gene

179 Adenoviral (AdV) vectors encoding either the lacZ gene or delta-sarcoglycan gene were constructed.

180 , driving expression of the Escherichia coli lacZ gene or the gene for the enhanced green fluorescent

181 promoter/enhancer, encoding nuclear-specific LacZ gene (pCMV-nlsLacZ) (50 microg/ml) to a single LV m

182 xon 1 and intron 1 with the Escherichia coli lacZ gene, placing lacZ under control of the Fmr2 promot

183 mechanism of hmuO regulation, a promoterless lacZ gene present on the promoter-probe vector pCM502 wa

184 creased as indicated by blue staining of the lacZ gene product, beta-galactosidase.

185 ells, these cells were also positive for the lacZ gene product.

186 -deficient retroviral vectors containing the lacZ gene, providing a permanent label that is transmitt

187 Expression of a transduced lacZ gene (rAAV/CMVlacZ) is silenced in greater than 90%

188 Furthermore, using an in vitro plasmid LacZ gene reactivation assay to determine DSB repair fid

189 nsgenic mice were established containing the lacZ gene regulated by the MIEP (nucleotides -670 to +54

190 a transgenic Samd9L(+/-) mouse in which the LacZ gene replaced exon 2 in the Samd9L gene.

191 serted between the Escherichia coli phoA and lacZ genes, respectively, to generate operon fusions to

192 event between two complementarily defective lacZ genes resulted in the production of beta-galactosid

193 cells and Agtr1a intact cells expressing the lacZ gene (ROSA26).

194 beta-Gal expression from the LacZ gene seems to parallel Tst-1/SCIP/Oct-6 expression

195 ecause fusion of the cum1(+) promoter to the lacZ gene showed that activation of the reporter gene oc

196 Studies of promoter fusion to a promoterless lacZ gene showed that compared to in vitro-grown bacteri

197 denovirus expressing the beta-galactosidase (LacZ) gene showed significantly lower tumor uptake value

198 affect the expression of the closely linked lacZ gene, suggesting that the insulator does not propag

199 a recombinant adenovirus vector carrying the lacZ gene survive grafting into the injured spinal cord

200 nohistochemical study of taste cells in BDNF(LacZ) gene targeted "knock-in" adult mice.

201 1.4-2.6-fold higher mutant frequency in the lacZ gene than mice not carrying the transgene.

202 In addition, mice with a lacZ gene that had been introduced into the Gsc locus we

203 riptional fusions of the same sequences to a lacZ gene that retained the lacZ ribosome-binding site w

204 utations in the Escherichia coli chromosomal lacZ gene that revert via deletion of a short duplicated

205 Expression of the lacZ gene that was under control of the promoter for nat

206 scriptional and translational fusions to the lacZ gene, that both putative transcriptional start site

207 o alpha 2(I) collagen promoter linked to the lacZ gene, the cis-acting elements responsible for enhan

208 where the Tie2 promoter drives the reporter LacZ gene (Tie2-LacZ), we demonstrate that at steady sta

209 on at the first intragenic terminator in the lacZ gene (tiZ1) which can be overcome by NusG: Rho can

210 ene to assess enhancer blocking and an hsp70-lacZ gene to examine the structure of a heat shock puff

211 E.U2V variant were ligated to a promoterless lacZ gene to prepare translational fusions for use as re

212 th a NF-kappaB responsive promoter driving a LacZ gene to produce beta galactosidase) demonstrated ne

213 cts high-level expression of reporter GATA-1/lacZ genes to primitive and definitive erythroid cells a

214 with unmodified and (especially) control Ad-LacZ gene-transduced DC.

215 that herpes simplex virus amplicon-mediated LacZ gene transfer into tumors can be transiently and th

216 icities of infection and the efficiencies of lacZ gene transfer were observed.

217 use models for BBS resulting from a targeted LacZ gene trap allele (Bbs5-/-) that is a predicted cong

218 In this study, we generated an Il-33-LacZ gene trap reporter strain (Il-33(Gt/Gt)) and used t

219 virus HD-2, containing the Escherichia coli lacZ gene under control of the HSV ICP8 early gene promo

220 is vector also contains the Escherichia coli lacZ gene under control of the thymidine kinase promoter

221 d a line of transgenic mice that express the LacZ gene under the control of an NF-kappaB-regulated pr

222 stable C2C12 cell lines carrying the nuclear lacZ gene under the control of the mouse epsilon subunit

223 A medium-copy-number plasmid carrying the lacZ gene under the control of the native sigma-54 promo

224 nsgenic mice expressing the fused mutant ICE-lacZ gene under the control of the neuron specific enola

225 a reporter cell line T1H6 that contains the lacZ gene under the control of the polyadenylated nuclea

226 polymerase is required for the expression of lacZ gene under the control of virB promoter (virBp::lac

227 We constructed an rAAV containing the LacZ gene under the transcriptional control of the cytom

228 Double-reporter constructs with the lacZ gene under transcriptional control of the ilvC prom

229 by joining exon 8 of Mll with the bacterial lacZ gene using homologous recombination in mouse embryo

230 veral nucleotides deleted within the E. coli lacZ gene was allowed time to repair in lacZ-expressing

231 The regional expression of the lacZ gene was also analyzed during embryonic and postnat

232 t either time point, mutant frequency in the lacZ gene was at least 40-fold higher in MeIQx-treated m

233 p promoter region of P. pantotrophus and the lacZ gene was constructed.

234 The expression of the lacZ gene was detected in melanoblasts and precursors of

235 In addition, the transient expression of the lacZ gene was detected in the brain of G0 zebrafish embr

236 The expression of LacZ gene was induced eight times and of VEGF 20 times i

237 The expression of the LacZ gene was induced in bitransgenic mice by administra

238 veral nucleotide deletion within the E. coli lacZ gene was introduced for repair in lacZ-expressing m

239 sion of the 199-bp region and a promoterless lacZ gene was introduced into Escherichia coli, promoter

240 ess of sequence, injection of dsRNA from the lacZ gene was likewise effective in strongly reducing tb

241 cZ recombinants was constructed in which the lacZ gene was regulated by either late (the AmEPV sphero

242 The beta-galactosidase reporter (lacZ gene) was targeted to the beta1 locus, which provid

243 ant retrovirus encoding the Escherichia coli lacZ gene, we have found that medium conditioned with NI

244 ng transcriptional fusions to a promoterless lacZ gene, we identified two independent promoters relat

245 onal fusions of dprA to the Escherichia coli lacZ gene, we show that expression of dprA::lacZ require

246 recombinant containing the Escherichia coli lacZ gene were applied to the abraded skin of the dorsal

247 Mutations in the lacZ gene were constructed that allowed the detection of

248 LP promoter upstream from a nuclear-targeted LacZ gene were generated, and expression was detected in

249 T binding sites control transcription of the lacZ gene, which codes for the enzyme beta-Galactosidase

250 A reporter gene, the bacterial lacZ gene, which expressed beta-galactosidase, was inser

251 By delivering the ICE-lacZ gene within a retroviral vector and under the contr