ライフサイエンスコーパス: lactase

1 parison with previous studies of sucrase and lactase.

2 e lactose in milk more efficiently than free lactase.

3 75% for free lactase to<15% for encapsulated lactase.

4 embrane disruption to physically release the lactase.

5 s with genetic hypolactasia, explain the low lactase activities commonly found in malnourished infant

6 e in digestion and absorption was related to lactase activity (P=0.034, R2=0.38).

7 ubjects, we found an association between low lactase activity and abdominal pain (OR = 1.78; 95% CI =

8 In vitro studies of intestinal lactase activity and breath-hydrogen studies have sugges

9 bjective was to select a probiotic with high lactase activity and compare it with lactase and placebo

10 d yogurts appear to exhibit somewhat reduced lactase activity but are still well tolerated.

11 Lactase activity correlated with gestational age at birt

12 but in most mammals, including most humans, lactase activity declines after the weaning phase.

13 Lactose digestion and absorption and lactase activity doubled between studies (P=0.035 and P=

14 ldhood, but in European-derived populations, lactase activity frequently persists into adulthood.

15 Bacterial cultures were screened for lactase activity in a model of the upper gastrointestina

16 Lactase activity is high and vital during infancy, but i

17 In other healthy humans, lactase activity persists at a high level throughout adu

18 es in lactose absorption relate primarily to lactase activity rather than to mucosal growth.

19 in was found only in adult animals (with low lactase activity), and there was no relationship between

20 elating to lactose digestion and absorption, lactase activity, and small-intestinal mucosal growth.

21 e directly lactose digestion and absorption, lactase activity, and small-intestinal surface area in p

22 ourished controls with normal morphology and lactase activity.

23 o be good proxies for genetically determined lactase activity.

24 dition of sodium caseinate further preserved lactase activity.

25 , honey, fruit preparations, novel cultures, lactase addition, inulin fiber addition, and flavor inte

26 glutaminase eliminated the detection of free lactase after freeze-drying emulsions and the addition o

27 human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold

28 are not detectable, despite the presence of lactase and phlorizin active sites in the polypeptide ba

29 th high lactase activity and compare it with lactase and placebo in clinical trials.

30 Lactase and sucrase enzyme proteins and activities were

31 We see strong signals of selection at lactase and the major histocompatibility complex, and in

32 enterocyte integral membrane proteins using lactase as a prototype, and examined the possibility tha

33 There is little difference in the lactase capability of different commercial yogurts, beca

34 The levels of sucrase, maltase, and lactase decreased in wild-type mice p.i. with the GS str

35 Congenital lactase deficiency (CLD) is a rare severe autosomal rece

36 rst genetically confirmed case of congenital lactase deficiency in Central Europe.

37 Congenital lactase deficiency is an extremely rare gastrointestinal

38 This case demonstrates (a) that congenital lactase deficiency should be considered in cases of seve

39 ature termination of translation, congenital lactase deficiency was confirmed and intestinal biopsies

40 Lactase deficiency was found in 34.3% of patients and di

41 Disaccharidase deficiency, particularly lactase deficiency, is common in youth with abdominal pa

42 used watery diarrhoea, suggesting congenital lactase deficiency.

43 However, in lactase-deficient individuals, lactose feeding supports

44 illebrand Factor (vWF) (p = 0.016) and serum lactase dehydrogenase (LDH) (p = 0.026).

45 .01) and high IL-10 was correlated with high lactase dehydrogenase (P = .0085) and higher Internation

46 Simultaneously, cytotoxicity was assessed by lactase dehydrogenase leakage assay.

47 We sequenced the lactase enhancer region in 457 individuals from 18 Khois

48 hort tandem repeat (STR) loci that flank the lactase enhancer region.

49 5, which has been previously associated with lactase expression and lactose tolerance, had higher die

50 In conclusion, endogenous lactase expression does not depend on the presence of di

51 ies that have attempted to induce intestinal lactase expression with different lactose feeding protoc

52 that derived alleles (at known enhancers for lactase expression) sit on an extended haplotype backgro

53 lactase non-persistence (LNP) is due to low lactase expression, resulting in lactose malabsorption (

54 s not, however, explain all the variation in lactase expression.

55 results from transcriptional suppression of lactase expression.

56 ose from the diet does not reduce intestinal lactase expression.

57 same LCT enhancer region can cause continued lactase expression.

58 n induced a spurious association between the lactase gene (LCT) and tall/short status in a European A

59 n vivo transcriptional gradient of the mouse lactase gene (Lct), which occurs in enterocytes along th

60 Nuclear protein bound to the lactase gene cis element, CE-LPH1, was analyzed by elect

61 Europeans at the 2q21.3 locus harboring the lactase gene has been attributed to selection for the ab

62 tence continue to express high levels of the lactase gene into adulthood.

63 To identify regions of the lactase gene involved in mediating the spatiotemporal ex

64 Transcription of the lactase gene is activated during enterocyte differentiat

65 acts as an enhancer to the expression of the lactase gene LCT is responsible for lactase persistence

66 riants was cloned upstream of the 3.0 kb rat lactase gene promoter in a luciferase reporter construct

67 after exclusion of primary LM by a negative lactase gene test.

68 Lactase gene transcription is spatially restricted to th

69 study concludes that malnutrition suppresses lactase gene transcription or mRNA stability in infants.

70 nal role for the polymorphisms in regulating lactase gene transcription.

71 most closely mimicked that of the endogenous lactase gene with respect to spatiotemporal restriction.

72 e 2, the most extreme signal is found in the lactase gene, which previously has been shown to be unde

73 ide polymorphisms covering 3.2 Mb around the lactase gene.

74 d by a polymorphic element cis-acting to the lactase gene.

75 variant in the cis-regulatory element of the lactase gene.

76 ome Diversity Project, and the analysis of a lactase-height dataset, we show that our method can corr

77 cally, miR-30d regulates the expression of a lactase in Akkermansia muciniphila, which increases Akke

78 upstream from the start of transcription of lactase in an intron of the adjacent gene MCM6.

79 th placebo: 0.25, P = 0.007) were lower with lactase in Booster Alpha.

80 emulsions as delivery systems with retained lactase in milk and controlled release during in vitro d

81 re potential delivery systems to incorporate lactase in milk products.

82 Persistence of intestinal lactase into adulthood allows humans to use milk from ot

83 The persistent expression of lactase into adulthood in humans is a recent genetic ada

84 ersistence (LP), the continued expression of lactase into adulthood, is the most strongly selected si

85 Lactase is an enzyme that hydrolyzes lactose into glucos

86 Synthesis of lactase is not affected by any of the cytokines.

87 Lactase is the intestinal disaccharidase responsible for

88 omization was applied in a subsample via the lactase LCT-13910 C/T single nucleotide polymorphism tha

89 A SNP in the gene encoding lactase (LCT) (C/T-13910) is associated with the ability

90 esolve evolutionarily important enhancers of lactase (LCT) and insulin-like growth factor binding pro

91 association between Bifidobacterium and the lactase (LCT) gene locus and identify an association bet

92 One locus, the lactase (LCT) gene locus, reached study-wide significanc

93 [polymorphism (rs4988235) upstream from the lactase (LCT) gene], where TT and TC genotypes are assoc

94 In malnourished infants, lactase messenger RNA (mRNA) was reduced to 32% and sucr

95 promoter and results in increased endogenous lactase messenger RNA.

96 The reductions of lactase mRNA, distinct from those found in adults with g

97 adequate to explain the greater reduction of lactase mRNA, this study concludes that malnutrition sup

98 Adult lactase non-persistence (LNP) is due to low lactase expr

99 nding the C_(13910) variant, associated with lactase non-persistence, results in a 2.2-fold increase

100 We propose that lactase non-persistent individuals consumed milk when it

101 een the low metabolite responses and genetic lactase nonpersistence (accuracy 0.92), galactitol and g

102 ly 70%-100% of the Asian adult population is lactase nonpersistent (LNP).

103 The effect of dietary lactose in lactase nonpersistent individuals on gut microbiota.

104 mL) per day produced negligible symptoms in lactase-nonpersistent (LNP) individuals self-described a

105 Bifidobacteria are associated with the human lactase nonpersister genotype, which typically confers l

106 lerance, where 2 x 1012 CFUs Bi-07, 4662 FCC lactase, or placebo was consumed simultaneously with a l

107 n intron 13 of the MCM6 gene associated with lactase persistence (i.e. the ability to digest the lact

108 Eastern Africa harbors distinctive lactase persistence (LP) alleles, and therefore LP allel

109 galactose/creatinine) discriminated between lactase persistence (LP) and LNP following lactose chall

110 We genetically defined lactase persistence (LP) in 31 720 individuals from eigh

111 Lactase persistence (LP) is a genetically-determined tra

112 ddle Eastern and southern Asian populations, lactase persistence (LP) is the most strongly selected m

113 </= 20,089) evaluated associations between a lactase persistence (LP) SNP, the minichromosome mainten

114 intake maps closely onto the distribution of lactase persistence (LP), a genetic trait that allows mi

115 Lactase persistence (LP), the continued expression of la

116 ary livestock products, and the evolution of lactase persistence (LP), which allows digestion of lact

117 ability to drink milk into adulthood through lactase persistence (LP).

118 also have influenced the evolution of adult lactase persistence across Europe.

119 the LCT locus predates the emergence of the lactase persistence allele by thousands of years.

120 ery residues, faunal mortality profiles, and lactase persistence allele frequencies, provide a partia

121 Finally, we find that the lactase persistence allele had a large positive effect o

122 n of the lactase gene LCT is responsible for lactase persistence and appears to have been under stron

123 en the high metabolite responses and genetic lactase persistence and between the low metabolite respo

124 here TT and TC genotypes are associated with lactase persistence and CC with nonpersistence.

125 3915 and C/G-13907) that are associated with lactase persistence and that have derived alleles that s

126 However, individuals with lactase persistence continue to express high levels of t

127 veness of our method on HapMap-simulated and lactase persistence datasets, where we significantly out

128 roduct tests through the association between lactase persistence genotype and the postprandial dynami

129 Lactase persistence has been strongly correlated with si

130 an evolutionary context for the emergence of lactase persistence in Africa.

131 ence) in Europeans, but the genetic basis of lactase persistence in Africans was previously unknown.

132 T, -13915T>G, -14010G>C) are associated with lactase persistence in different populations.

133 0,000 years, consistent with an advantage to lactase persistence in the setting of dairy farming; the

134 Lactase persistence is a heritable, autosomal dominant,

135 hat is frequent in Northern Europeans, where lactase persistence is frequent.

136 strength and timing of natural selection on lactase persistence or height, among others.(6)(,)(11)(,

137 flected in the rise of the allele conferring lactase persistence to approximately 50% by this time co

138 two alleles that are tightly associated with lactase persistence uniquely mark a common (~77%) haplot

139 ed in some cases, such as lactose tolerance (lactase persistence) in adults, but is less well underst

140 d with the ability to digest milk as adults (lactase persistence) in Europeans, but the genetic basis

141 ns and at known positive control loci (e.g., lactase persistence), G12 outperforms the allele frequen

142 ry sequence in the MCM6 gene associated with lactase persistence, a human trait tied to the cultural

143 er positive selection, including skin color, lactase persistence, and resistance to malaria.

144 These include those coding for lactase persistence, blue eye color, Y chromosome R1b ha

145 e, -14009*G, has borderline association with lactase persistence, but loses significance after correc

146 ciated variants, including those linked with lactase persistence, likely reflecting ancestry change r

147 The T_(13910) variant, associated with lactase persistence, results in an even greater 2.8-fold

148 architectures underlying traits ranging from lactase persistence, to skin pigmentation, to hypoxic re

149 minantly inherited genetic trait is known as lactase persistence.

150 (rs4988235) was performed to assess primary lactase persistence.

151 -obesity, observationally or genetically via lactase persistence.

152 n and surprisingly, no Neolithic presence of lactase persistence.

153 date) are each significantly associated with lactase persistence.

154 usative role in the mechanism specifying the lactase persistence/non-persistence phenotypes in humans

155 The DNA region of the C/T_(13910) lactase persistence/non-persistence variant functions in

156 enetic polymorphisms closely associated with lactase persistence/nonpersistence have been identified.

157 lactose into adulthood (i.e., they have the lactase-persistence [LP] trait).

158 trace positively selected loci-including the lactase-persistence allele of LCT and alleles of ANKA th

159 However, a number of lactase persistent individuals carry none of these allel

160 the enhancer is significantly higher in the lactase persistent members of this and a second cohort c

161 o denied lactose intolerance (A-LNP), and 10 lactase-persistent individuals who believed they were la

162 The higher risk of type 2 diabetes in lactase-persistent individuals without milk intake likel

163 The distribution of these different lactase phenotypes in human populations is highly variab

164 r beta-glucosidase in the hydrolysis of PNG, lactase phlorizin hydrolase (LPH) was purified from rat

165 ture stop codons in the coding region of the lactase-phlorizin hydrolase (LPH) gene.

166 Lactase-phlorizin hydrolase (LPH) is an absorptive enter

167 liver fatty acid binding protein (Fabp1) and lactase-phlorizin hydrolase (LPH), and a surprising indu

168 Sucrase-isomaltase (SI), lactase-phlorizin hydrolase (LPH), and neutral Aminopept

169 ecline in production of the digestive enzyme lactase-phlorizin hydrolase during maturation.

170 PH1-binding proteins do not seem to regulate lactase-phlorizin hydrolase expression during this perio

171 Sucrase-isomaltase and lactase-phlorizin hydrolase expressions change remarkabl

172 e nuclear proteins to sucrase-isomaltase and lactase-phlorizin hydrolase gene expression in rats duri

173 to the CE-LPH1 cis-regulatory element of the lactase-phlorizin hydrolase gene, in this regulation.

174 hip between enzymatic activity and levels of lactase-phlorizin hydrolase mRNA.

175 r interruption of HNF-1-binding sites in the lactase-phlorizin hydrolase promoter resulted in a compl

176 necessary for cooperative activation of the lactase-phlorizin hydrolase promoter.

177 g because of decreasing levels of the enzyme lactase-phlorizin hydrolase, encoded by LCT.

178 Spray-dried lactase powder was suspended in anhydrous milk fat/Span(

179 Lactase promoter activities were assayed in cells transf

180 rsistence, results in a 2.2-fold increase in lactase promoter activity.

181 ve characterized the interaction between the lactase promoter and Cdx2, a homeodomain protein involve

182 homeodomain protein Cdx2 interacts with the lactase promoter and is capable of activating transcript

183 a previous report, Cdx2 interaction with the lactase promoter correlates with enterocyte differentiat

184 Thus, a distinct 5'-region of the lactase promoter directs intestine-specific expression i

185 erns of a luciferase reporter gene driven by lactase promoter regions in transgenic mice.

186 Two independent, 1.3- and 2.0-kb, lactase promoter-reporter transgenic lines expressed app

187 fferential transcriptional activation of the lactase promoter.

188 Many malnourished infants have reduced lactase specific activity in the small intestine.

189 Lactose digestion and absorption, lactase-specific activity, and lumen-to-mucosa water flu

190 or dairy consumption is a poor indicator of lactase status, corroborating the results of interventio

191 8), located 13.9 and 22 kb upstream from the lactase structural gene.

192 Activities of lactase, sucrase, maltase, and palatinase consistently e

193 Disaccharidase assays tested for activity of lactase, sucrase, maltase, and palatinase.

194 presence of functional brush-border enzymes (lactase, sucrase-isomaltase and dipeptidyl peptidase 4)

195 Subjects with a lactase/sucrase ratio < 0.2 were found to be associated

196 The bacterial lactase survives the acidic conditions of the stomach, a

197 shed children was associated with much lower lactase than sucrase mRNA abundance and because the epig

198 The enzyme lactase that is located in the villus enterocytes of the

199 to 4500 Food Chemicals Codex (FCC) units of lactase, the amount in the European Food Safety Authorit

200 , ~70% of adults are deficient in intestinal lactase, the enzyme required for the digestion of lactos

201 ointestinal transit time allow the bacterial lactase to be active, digesting lactose from yogurt suff

202 ter 3-week storage reduced from>75% for free lactase to<15% for encapsulated lactase.

203 on of a neighboring gene (MCM6) and modulate lactase transcription in vitro.

204 localized to a 1.2-kb region upstream of the lactase transcription start site.

205 milk intake was 5 glasses/wk (IQR: 0-10) for lactase TT/TC persistence and 3 (0-7) for CC nonpersiste

206 Genetically for lactase TT/TC persistence compared with CC nonpersistenc

207 tinal Caco-2 cells were transfected with the lactase variant/promoter-reporter constructs and assayed

208 gion of the C/T_(13910) or G/A_(22018) human lactase variants was cloned upstream of the 3.0 kb rat l

209 Noninferiority of Bi-07 compared with lactase was observed in Booster Omega (0.460; 95% CI: 0.

210 The encapsulated lactase was released gradually during the simulated dige

211 Lactase was superior to placebo in Booster Alpha (0.190;