ライフサイエンスコーパス: lethal mutation

1 t position 102 or 104 of RKLKR resulted in a lethal mutation.

2 ced in cis with the plasmid-encoded dominant lethal mutation.

3 led that Mom2R encodes a recessive embryonic lethal mutation.

4 the viral gene encoding either gM or gN is a lethal mutation.

5 for circumventing the effects of potentially lethal mutations.

6 mate of assumptions, such as the fraction of lethal mutations.

7 used to molecularly map previously isolated lethal mutations.

8 for studying the maternal effects of zygotic lethal mutations.

9 nd numbers of genes carry similar numbers of lethal mutations.

10 2F alleles among a collection of EMS-induced lethal mutations.

11 ntralized phenotype similar to dpp embryonic lethal mutations.

12 is shown to complement yeast GUK1 recessive lethal mutations.

13 families and obtained 9 recessive embryonic lethal mutations.

14 screens been conducted to isolate embryonic lethal mutations.

15 ing the maternal effect of recessive zygotic lethal mutations.

16 orates beneficial, neutral, deleterious, and lethal mutations.

17 e's essential genes by temperature-sensitive lethal mutations.

18 an adaptive mutant strain are threatened by lethal mutations.

19 GC transitions, which can eventually lead to lethal mutations.

20 ) by recombinational rescue of conditionally lethal mutations.

21 ific chromosomal translocations can generate lethal mutations.

22 for example synthetic phage genomes carrying lethal mutations.

23 ially leading to the stable incorporation of lethal mutations.

24 intain and analyze stocks carrying recessive lethal mutations.

25 rtion mutations, enabling us to recover 2500 lethal mutations.

26 Moreover, none are intact; all have lethal mutations [1, 3, 4].

27 Because of the prevalence of dominant lethal mutations, a comprehensive fitness landscape of t

28 We have screened for zygotic embryonic lethal mutations affecting cuticular morphology in Nason

29 to the isolation of two classes of recessive lethal mutations affecting early dorsoventral pattern fo

30 f G --> A excess and predicted indicators of lethal mutation and applied this model to 325 920 unique

31 between accessible functional groups of the lethal mutation and those of the viable mutation and wil

32 anced sensitivity to a temperature-sensitive lethal mutation and to the neurodegenerative effects pro

33 enous S. pombe gamma-tubulin, resulted in 11 lethal mutations and 12 cold-sensitive mutations.

34 fluorescent protein efficiently rescues cac lethal mutations and allows in vivo analysis of calcium

35 t greatly enhances the rate of appearance of lethal mutations and dominant female sterility.

36 initiated with the isolation and mapping of lethal mutations and genome rearrangements in the region

37 With screens for orc2-1 synthetic lethal mutations and Orc2p two-hybrid interactors, a nov

38 n against hepatitis C virus (HCV) by causing lethal mutations and suppressing RNA polymerase in vitro

39 ed to analyse the phenotype of the recessive-lethal mutations and used to show that they blocked cell

40 the presence of nearby proline residues and lethal mutations, and the presence of nearby alanines re

41 osin II heavy chain, and cells carrying this lethal mutation are defective in actomyosin ring assembl

42 Here we describe a recessive lethal mutation, arkadia, generated using gene-trap muta

43 We present a strategy that allows mapping of lethal mutations, as well as viable mutations with visib

44 In a screen for zygotic lethal mutations associated with maternal effects, we ha

45 S7 is a dominant temperature-sensitive (DTS) lethal mutation at 29 degrees that also acts as a recess

46 proximity, a mild mutation at site 901 and a lethal mutation at site 913.

47 In contrast, lethal mutations at the contact with p15/ARPC5 led to un

48 Lethal mutations at the contact with p19/ARPC4 specifica

49 characterization of a temperature-sensitive lethal mutation, bamAE373K, which alters the fifth polyp

50 r identifying and studying recessive zygotic lethal mutations because unfertilized eggs develop as ma

51 ctures, having a viable (C2658U*G2663A) or a lethal mutation (C2658G*G2663C), were determined at 1.75

52 Therefore, the slight deficit of a non-lethal mutation can be detected and characterized.

53 development, demonstrate that X-linked male lethal mutations can be recovered from ENU mutagenesis s

54 Finally, we characterize an embryonic lethal mutation caused by endogenous splicing disruption

55 Lethal mutations clustered in the amino-terminal half of

56 Classes of mutants include conditional lethal mutations, conditional auxotrophs, and conditiona

57 viability due to the uncovering of recessive lethal mutations correlated with an increase in gross ch

58 R4 use is constrained by relatively frequent lethal mutations, deep fitness valleys, and requirements

59 ating E2-Torsin-1A interaction constitutes a lethal mutation, demonstrating that this virus-host prot

60 here on pickwickm171 (pikm171), a recessive lethal mutation discovered in a large-scale genetic scre

61 Previously, a dominant temperature-sensitive lethal mutation, dnaT1, had been isolated in E. coli 15T

62 N-induced cytidine deaminase that introduces lethal mutations during retroviral reverse transcription

63 Three new recessive lethal mutations, E170A, D194A, and R206A, were identifi

64 e yielded five recessive csl (cep1 synthetic lethal) mutations, each defining a unique complementatio

65 al chromosomes have nevertheless accumulated lethal mutations, excess non-synonymous mutations, and e

66 Two new recessive lethal mutations, F256A and Y330A, were identified.

67 rosophila through screens of maternal effect lethal mutations for defects in spindle pole behaviour.

68 lethal phenotype and separated the recessive lethal mutation from the P-element by recombination.

69 Larval lethal mutations generated by ENU mutagenesis affect dif

70 thers are homozygous for the maternal effect lethal mutation gnu (GNU embryos) under DNA synthesis bu

71 these, 455 correspond to genes for which no lethal mutation has yet been reported.

72 Exquisite embryonic lethal mutations have been isolated in hundreds of genes

73 riment, in order to estimate the load due to lethal mutations (i.e. the lethal mutation rate), we man

74 in that acted as intragenic suppressors of a lethal mutation (I485N) mapping to the peptide-binding d

75 ntation analysis of P23 RNA that contained a lethal mutation in 2C confirmed these results.

76 In addition, a mutant with a conditional lethal mutation in lpxC, an essential gene required for

77 udy as to how fish tolerate what is an early lethal mutation in mammals could facilitate improvement

78 That the PE deletion is a lethal mutation in normative environments suggests that

79 As predicted by the genetic results, a lethal mutation in region I resulted in loss of Est3p fr

80 In BirA, G154D was a lethal mutation in single copy, and the purified protein

81 naturally occurring, recessive, perinatally lethal mutation in the aggrecan core protein gene, cmd(b

82 From a genetic mosaic screen, we find that a lethal mutation in the Drosophila Down syndrome cell adh

83 The presence of the same lethal mutation in the env genes of both FeLV and murine

84 r growth mutant orb2-34, which carries a non-lethal mutation in the essential gene shk1(+)/pak1(+)/or

85 ppressors of LG4, a virus with a conditional lethal mutation in the gene encoding ICP27.

86 distant intragenic suppressor of a dominant lethal mutation in the guanine nucleotide-binding region

87 omosome because it can be used to maintain a lethal mutation in the inversion interval as a self-sust

88 f-function, temperature-sensitive, embryonic lethal mutation in the maternally required gene gad-1 (g

89 Analysis of recombinant viruses carrying a lethal mutation in the NES of ICP27 was not accomplished

90 Somatic homozygous clones of an embryonic lethal mutation in this gene (stru1A122) cause wing blis

91 A lethal mutation in trol results in the failure of quiesc

92 infectious virions containing the otherwise lethal mutation in VP1.

93 We recently characterized a lethal mutation in ytr, a conserved gene that encodes a

94 Not really finished (nrf), a larval-lethal mutation in zebrafish generated by retroviral ins

95 onal alleles of foxi one (foo), an embryonic lethal mutation in zebrafish that displays defects in bo

96 An analysis of lethal mutations in a PS integrin gene showed that the i

97 Two conditionally lethal mutations in bimD arrest with aberrant mitotic sp

98 In screening for embryonic-lethal mutations in Caenorhabditis elegans, we defined a

99 dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesi

100 Using this system, several lethal mutations in CHIKV nsP1 were shown to reduce but

101 ic transmission, we have isolated homozygous lethal mutations in Drosophila importin 13 (imp13).

102 Propagation of viruses bearing those lethal mutations in G completely depended on complementa

103 authentic recombinant VSV particles bearing lethal mutations in G, confirms that the hydrophobic fus

104 Recently, we reported a genetic screen for lethal mutations in gene 2.5 that we are using to identi

105 A screen for lethal mutations in gene 2.5 uncovered a variety of esse

106 complementation assay was used to screen for lethal mutations in gene 2.5.

107 sh have led to the isolation of thousands of lethal mutations in genes that are essential for embryon

108 mutagenesis was used to generate conditional-lethal mutations in hitherto-uncharacterized domains of

109 within conserved motifs and demonstrate that lethal mutations in predicted ATP binding-hydrolysis mot

110 ly and identified second-site suppressors of lethal mutations in SP.

111 ned the phenotypes of strong and weak larval lethal mutations in spaghetti squash (sqh), which encode

112 h to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second chromos

113 Moreover, phenotypically lethal mutations in the carboxyl-terminal WD-40 repeats

114 rformed genetic screens to isolate recessive lethal mutations in the chromosomal region that includes

115 ribe new myo2 alleles containing conditional lethal mutations in the COOH-terminal tail domain.

116 sites in the genome, allowing the rescue of lethal mutations in the corresponding genes.

117 n's structure and function, we identified 13 lethal mutations in the Drosophila kinesin heavy chain m

118 have used previously characterized recessive lethal mutations in the dynein heavy chain gene, Dhc64C,

119 Lethal mutations in the extended arm drastically reduced

120 uthentic recombinant viral particles bearing lethal mutations in the G gene.

121 We show here that maternal-effect lethal mutations in the gene mex-3 cause descendants of

122 A binding, we isolated an assortment of heat-lethal mutations in the genes encoding RPA2 and RPA3.

123 tions of HSF in Drosophila, we have isolated lethal mutations in the hsf gene.

124 Lethal mutations in the region are suppressed by Cus1-54

125 carried out a large-scale genetic screen for lethal mutations in the region.

126 We have studied lethal mutations in the single calmodulin gene (Cam) of

127 the utility of the method by applying it to lethal mutations in the synaptic transmission genes syna

128 Furthermore, the non-lethal mutations in these essential genes suggest new st

129 nduced mutations vary significantly from non-lethal mutations in virus to localized hypermutations in

130 uman DNA genomes vary significantly from non-lethal mutations in viruses to localized hypermutations,

131 large-scale mutagenesis screen for embryonic lethal mutations in zebrafish Danio rerio we have found

132 genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 muta

133 Whereas mutations in Dm-myb are lethal, mutations in mip130 are viable.

134 Lethal mutations, in particular those that perturbed par

135 expressed in mast cells, including embryonic lethal mutations, in vitro or in vivo.

136 tion groups were defined by seven prenatally lethal mutations, including a group (l7R3) comprised of

137 we report that two independent, postnatally lethal mutations induced by N-ethyl-N-nitrosourea and ma

138 Conditional lethal mutations inserted into mosquito genomes allow fo

139 nt bacterial strains do not evolve because a lethal mutation is required to gain immunity.

140 Surprisingly, five lethal mutations lie outside all polymerase homology in

141 Cx26-G45E is a lethal mutation linked to KIDS that forms constitutively

142 oids, the presence of PRR alone may confer a lethal mutation load or, alternatively, PRR alone may be

143 mutations may be useful for balancing other lethal mutations located in the distal region of LG 12.

144 We describe a lethal mutation mouse strain generated using promoter-tr

145 We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene t

146 mutagenesis of lambda's A gene and found ten lethal mutations, nine of which cause post-cleavage pack

147 sociated with nucleotide binding sites where lethal mutations occur.

148 A non-lethal mutation of CFD1 (cfd1-1) reduced c-aconitase spe

149 d DNA strand-exchange and rescuing the ssb-1 lethal mutation of E. coli respectively.

150 A recently isolated, lethal mutation of the homeotic Abdominal gene of the re

151 egulatory protein, 2A(pro), for an otherwise lethal mutation of the structural VP1 protein to facilit

152 The NtPSA1 cDNA was used to complement a lethal mutation of the yeast PRC1 alpha subunit gene ind

153 Biochemical analysis showed that lethal mutations of Asp425, Arg463, Arg511 and His515 in

154 Screening for suppressors of dominant lethal mutations of essential genes is challenging as th

155 ted the ability of wild-type SCNM1 to rescue lethal mutations of I-mfa and Brunol4.

156 Genetic analyses reveal that lethal mutations of MAGUKs often occur in the guanylate

157 actor-independent peptide synthesis; nor did lethal mutations of nucleotides that abolish the binding

158 Recessive lethal mutations of the lethal(2)giant discs (l(2)gd) an

159 e use temperature-sensitive (ts) conditional lethal mutations of the S. cerevisiae POL2 and POL3 gene

160 diated by cytidine deamination, which causes lethal mutations of the viral genome.

161 regulate cell movement in vivo, we screened lethal mutations on chromosome 3R for defects in border

162 We conducted a mosaic genetic screen of lethal mutations on the Drosophila X chromosome to ident

163 e lines are segregating or are now fixed for lethal mutations on the mutagenized chromosome.

164 nk patterning and morphogenesis; we screened lethal mutations on the second chromosome for those that

165 infection in at least two ways: by inducing lethal mutations on the viral cDNA; and by blocking step

166 However, when we subsequently mapped the lethal mutations onto a model of the structure of the mu

167 se of an increased probability of generating lethal mutations or inducing apoptosis.

168 most resistant replicon was able to rescue a lethal mutation (P540A) in NS5B that disrupts its intera

169 s type of insertional mutagen is 1 embryonic lethal mutation per 70-100 proviral insertions, screenin

170 Interestingly, Thr121, the lethal mutation position in MFSD2A, forms stable interac

171 associate with each other, while one of the lethal mutations qkI(kt4) with a single amino acid chang

172 e viability may not be much greater than the lethal mutation rate (0.01 in these lines), but the resu

173 e the load due to lethal mutations (i.e. the lethal mutation rate), we manipulate thousands of indivi

174 ent-dependent dominant lethal, and recessive lethal mutation rates.

175 s the inbreeding load of both lethal and non-lethal mutations, reducing the amount of inbreeding depr

176 Finally, heterozygous DmORC2 recessive lethal mutations resulted in a suppression of PEV.

177 Zebrafish embryos with the recessive lethal mutations santa (san) and valentine (vtn) do not

178 We have isolated an embryonic lethal mutation, SBU2, that causes somite formation defe

179 L63-specific lethal mutations showed that L63 is required not only fo

180 d in these pairs show decreased frequency of lethal mutations, suggesting their specific role in male

181 that a subset of loss-of-function (LOF) and lethal mutations tended to increase distances of TL resi

182 alyronz12 (aln) is a recessive lethal mutation that affects early stages of neural cres

183 is plant carries a semidominant, conditional lethal mutation that confers constitutive expression of

184 amn) mouse, which has a recessive, embryonic lethal mutation that interferes specifically with the fo

185 In addition, recessive-lethal mutations that affect residues highly conserved o

186 een of >4000 mutagenized chromosomes bearing lethal mutations that affected multiple aspects of larva

187 Early screens for conditional lethal mutations that affected rRNA expression in Escher

188 Screens for zygotic lethal mutations that are associated with specific mater

189 o tolerate mutations rather than having more lethal mutations that are not observed.

190 tisubunit IN-viral DNA complex, we found the lethal mutations that caused virus morphogenesis defects

191 In previous work with phage lambda, lethal mutations that changed ATP-reactive residues 46 a

192 y chain gene was used to isolate EMS-induced lethal mutations that define at least eight essential ge

193 1 that uncouple metamorphosis, and embryonic-lethal mutations that map to common sequences encoding t

194 of visceral leishmaniasis, are conditionally lethal mutations that render the insect vector form of t

195 gene in a genetic screen to recover X-linked lethal mutations that require this transgene for viabili

196 However, many of these are perinatal lethal mutations that result in death from respiratory d

197 on in A. nidulans, we searched for synthetic lethal mutations that significantly reduced growth in th

198 ous mutagenesis studies identified recessive lethal mutations that were rescued by a genomic fragment

199 applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line.

200 e an obligate diploid that carries recessive lethal mutations throughout the genome.

201 that influence the effect of early embryonic lethal mutations, thus furthering knowledge of genetic i

202 domain are severely affected by the class II lethal mutations; thus, the mutant sequences should be v

203 viously mapped these X-linked dominant, male-lethal mutations to an overlapping region of 600 kb that

204 Embryonic lethal mutations (total of 34) were overwhelmingly the l

205 L5Jcs1 is a perinatal lethal mutation uncovered in a screen for ENU-induced mu

206 Further, cells with proviruses containing lethal mutations upstream of CTL epitopes can also be re

207 In its human homolog MFSD2A, a lethal mutation was mapped at its Na(+)-binding pocket;

208 A recessive embryonic lethal mutation was obtained.

209 A screen for synthetic lethal mutations was carried out with an rtf1 deletion m

210 other vector that is suitable for generating lethal mutations was constructed in a plasmid containing

211 ells that carry a temperature-sensitive cell-lethal mutation, we conditionally ablate patches of tiss

212 genetic approach to isolate lin-35 synthetic-lethal mutations, we have identified redundant roles for

213 ctions, a series of absolute and conditional lethal mutations were generated.

214 A total of 79 lethal mutations were isolated, representing at least 17

215 sociated with the lethal spotted and piebald lethal mutations which manifest only in the large intest

216 is similar to the lethal spotted and piebald lethal mutations, which are due to defects in endothelin

217 Maternal-effect lethal mutations with a partitioning defective phenotype

218 dentifying Mu insertion sites linked to seed-lethal mutations with a preliminary success rate of near

219 Therefore, an efficient approach to study lethal mutations would be useful.