ライフサイエンスコーパス: ligand binding

1 a general understanding of membrane-mediated ligand binding.

2 tly to prolong mRNA longevity in response to ligand binding.

3 The receptor also features a side pocket for ligand binding.

4 relates closely with the strength of aptamer-ligand binding.

5 hild analysis (pA(2) values) and fluorescent ligand binding.

6 metry, and conformational changes induced by ligand binding.

7 ing due to their fluorogenic properties upon ligand binding.

8 ion by conformational changes in response to ligand binding.

9 le of P450-P450 interactions and of multiple-ligand binding.

10 surface expression of alpha9alpha10 require ligand binding.

11 ly monomeric TNFR1, which exhibited impaired ligand binding.

12 g of major IgE and T-cell epitopes of BLG by ligand binding.

13 e amino acid being primarily responsible for ligand binding.

14 unliganded wild-type TNFR1 but exhibited no ligand binding.

15 ater displacement to contribute favorably to ligand binding.

16 egic positions is an important tool to study ligand binding.

17 c model of coupled conformational change and ligand binding.

18 on coactivator, orthosteric, and allosteric ligand binding.

19 imer-promoting conformations by mutations or ligand binding.

20 lso experiences a reduction in dynamics with ligand binding.

21 and transitions to protease-like forms upon ligand binding.

22 r region with an amphiphilic environment for ligand binding.

23 prebinding conformational change followed by ligand binding.

24 ative mass spectrometry (MS) and competitive ligand binding.

25 domain can regulate EAG channel function via ligand binding.

26 s ligands and what accessory proteins assist ligand binding.

27 information about the mechanisms of protein-ligand binding.

28 A metal ion also plays an important role in ligand binding.

29 sition to an open state that can accommodate ligand binding.

30 undergoes localized structural changes upon ligand binding.

31 f transcription elongation, RNA folding, and ligand binding.

32 significantly influencing the specificity of ligand binding.

33 ed peptides offered synergistic insight into ligand binding.

34 ular juxtamembrane region is reordered after ligand binding.

35 Upon ligand binding, a receptor proximal complex consisting o

36 ffect the protein's structure, dynamics, and ligand-binding activity in both its soluble and membrane

37 n dynamic cross-correlation maps showed that ligand binding affects the coupling between the C-loop a

38 ses, allowing the determination of essential ligand binding affinities (K(D)).

39 647-labeled conjugate allowed measurement of ligand binding affinities of unlabeled hA(2A)AR antagoni

40 ts in two forms: trans Pro300 SthK with high ligand binding affinity and fast activation, and cis Pro

41 l penalties can be major determinants of RNA-ligand binding affinity as well as a source of binding c

42 chine learning scoring functions for protein-ligand binding affinity prediction have been found to co

43 red to the wild type allele by measuring its ligand binding affinity, CCL2 scavenging efficiency, and

44 he receptor to agonists without changing the ligand binding affinity.

45 ptor response without substantially altering ligand binding affinity.

46 , locating small molecule binding, measuring ligand-binding affinity, monitoring protein folding and

47 ultimers, thereby reducing multimer size and ligand-binding affinity.

48 what we know about the molecular details of ligand binding, agonist-driven conformational changes, a

49 rlying membrane-protein functions, including ligand binding, allostery, and signaling.

50 r and six of its mutants, we determined that ligand binding alters the exonuclease digestion kinetics

51 om site-directed mutagenesis and competitive ligand-binding analyses revealed that DPO and Ala-AA occ

52 ighly variable, thus allowing differentiated ligand binding and activity of heparan sulfate proteogly

53 We tested the effect of ligand binding and amino acid sequence on the structure

54 ain a follistatin domain (FSD) important for ligand binding and antagonism.

55 These new strains have altered ligand binding and antigenicity characteristics.

56 different binding sites and coupling between ligand binding and conformational change.

57 a bidirectional allosteric coupling between ligand binding and coregulator binding that determines r

58 eterminants of signals transduced across the ligand binding and coregulator recruitment by all three

59 These findings uncover coupling between ligand binding and coregulator recruitment that affects

60 ptors with ozone-derived oxysterols impaired ligand binding and corresponded with reduced macrophage

61 gnaling inputs that destabilize this domain (ligand binding and demethylation) disfavor CheA binding

62 n monitoring photoinduced reaction cascades, ligand binding and dissociation, electron transfer, enzy

63 te profoundly influences beta2AR orthosteric ligand binding and downstream function.

64 work provides unique tools to modulate aGPCR-ligand binding and establishes a foundation for the deve

65 ic RNA aptamers and their different modes of ligand binding and fluorescence activation.

66 r large extracellular regions (ECRs) mediate ligand binding and function.

67 The R47H variant of human TREM2 impairs ligand binding and increases Alzheimer's disease (AD) ri

68 nsistent with a two-step mechanism involving ligand binding and isomerization either of the apo or th

69 rs (GPCR) requires thorough understanding of ligand binding and mechanism of activation through high

70 lthough much is known about the mechanics of ligand binding and PRR activation, how cells coordinate

71 ctuations in ECD conformation may affect the ligand binding and receptor activation properties.

72 ool compounds, enabling the visualization of ligand binding and receptor characterization both in vit

73 Here, we analyzed the correlation between ligand binding and receptor conformation of the alpha(1A

74 de force approach to systematically evaluate ligand binding and signaling properties ([(35)S]GTPgamma

75 Combined with mutagenesis, ligand binding and signaling studies, key interactions b

76 providing a potentially crucial link between ligand binding and the receptor core that engages G prot

77 on solid-state NMR spectroscopy for studying ligand binding and the surface structure of nanomaterial

78 el TRPM2 antagonist, tatM2NX, which prevents ligand binding and TRPM2 activation.

79 the separation of specific from non-specific ligand binding and, provides a suitable complement to ch

80 s to identify ligand-receptor pairs based on ligand binding and/or signal transduction could advance

81 We conclude that both ligand-binding and activity-based assays under optimized

82 could be modeled with ten intricately linked ligand-binding and conformational exchange reactions.

83 s and a detailed kinetic characterization of ligand-binding and dissociation revealed that the bindin

84 Ligand-binding and kinetic studies indicate severely red

85 nse to external stimuli including light, pH, ligand-binding and other microenvironmental cues.

86 ystallises, crystal packing often influences ligand-binding and protein-protein interaction interface

87 l to capture the coupling between proton and ligand binding, and conformational changes associated wi

88 f "one domain-one binding site", motif-based ligand binding, and coupled folding and binding of intri

89 al the variability of topology, influence of ligand binding, and glycosidic angle rearrangements seen

90 citly monitoring the conformational changes, ligand binding, and phosphorylation events that occur on

91 e of the actin cytoskeleton in the dynamics, ligand binding, and signaling of the serotonin(1A) recep

92 n retrospect, underlie different patterns of ligand binding, and that the biology of glutamate recept

93 g features critical for signal transduction, ligand binding, and voltage sensing.

94 t from the haem and the reverse process upon ligand binding are what ultimately drives the respirator

95 e resonance energy transfer (NanoBRET)-based ligand binding assay for SMO providing a sensitive and h

96 lizing a recently established NanoBRET-based ligand binding assay.

97 , sensitivity, and throughput of traditional ligand-binding assay (LBA) and liquid chromatography-tan

98 tible assay is superior to commonly used SMO ligand binding assays in the separation of specific from

99 Ligand binding assays routinely employ fluorescently-lab

100 Compared with ligand binding assays, a major advantage of mass spectro

101 otential to promote more accurate and robust ligand binding assays.

102 Using cell-based Notch signaling and ligand-binding assays, we evaluated differences in NOTCH

103 Because ligand binding at internal sites of DNA oligomers modula

104 tation did not affect surface expression and ligand binding but changed the susceptibility to heat de

105 ools to dimerize receptors in the absence of ligand binding, but high-fidelity receptor activation re

106 EphA2 induces antioncogenic signaling after ligand binding, but ligand-independent activation of Eph

107 g systemic administration, in order to block ligand binding by local CL-11 and prevent complement act

108 Ligand binding by other riboswitch aptamers peripheral t

109 sity changes of these isomers in response to ligand binding can be exploited to delineate ligand-prot

110 signaling through release of CNTFRalpha, the ligand-binding component of the CNTF-receptor multiprote

111 The structures illustrate homologies in the ligand-binding core but distinct peripheral tertiary con

112 witches alter gene expression in response to ligand binding, coupling sensing and regulatory function

113 rast, a form of TNFR1 with a mutation in the ligand-binding CRD2 subdomain retained the monomer-to-di

114 vely describe the mechanics of the molecular ligand binding/dissociation of the OTP.

115 om Lactobacillus rhamnosus demonstrates that ligand binding does not ensure successful gene regulatio

116 currences harbor activating mutations in the ligand binding domain (LBD) of ER, which have been shown

117 ative splicing and retains the extracellular ligand binding domain but lacks the intracellular signal

118 y assay showed that PB directly binds to the ligand binding domain of hPXR (K(D) = 1.42 x 10(-05)).

119 analyses reveal that 7j occupies the typical ligand binding domain of the PPARgamma agonists with, ho

120 H08), a radioligand for PET that engages the ligand binding domain on GR.

121 ACVR1-R206H and -G328R do not require their ligand binding domain to over-activate BMP signaling in

122 the structure of the glutamine-II riboswitch ligand binding domain using X-ray crystallography.

123 udies reveal that only subtle changes in the ligand binding domain, often identified only in retrospe

124 nts and cocrystallization with the RORgammat ligand binding domain.

125 r, we biochemically reconstituted the GLR3.3 ligand-binding domain (LBD) and analyzed its selectivity

126 Given that the ligand-binding domain (LBD) of PPARG is the same in kill

127 nding pocket in the C-terminal region of the ligand-binding domain (LBD) of RORgammat was discovered

128 a periplasmic NTD fused to the conventional ligand-binding domain (LBD).

129 al effects and the ability to bind the Nurr1 ligand-binding domain (LBD).

130 olling the tension of the linker between the ligand-binding domain and the transmembrane ion channel

131 t the crystal structure of the zebrafish VDR ligand-binding domain in complex with the ZK168281 antag

132 f the beta11-12 linker in the extracellular, ligand-binding domain is an integral component of the de

133 ncluding those with hotspot mutations in the ligand-binding domain of ERalpha, remain dependent on ER

134 their extracellular binding domains with the ligand-binding domain of metabotropic glutamate receptor

135 ificity mapped primarily to the cytoplasmic, ligand-binding domain of STING.

136 g of GK-15 into the N-terminal extracellular ligand-binding domain of the umami (T1R) receptor was pe

137 of a leucine by threonine in helix 8 of the ligand-binding domain of the zebrafish MR confers the an

138 In fact, the ERRbeta ligand-binding domain remains the last unsolved ERR stru

139 ar docking analysis of PioOH-bound PPARgamma ligand-binding domain revealed an altered hydrogen bondi

140 dicate that CITCO binds directly to the hPXR ligand-binding domain to activate hPXR.

141 number of riboswitches that utilize the same ligand-binding domain to regulate transcription or trans

142 g the linker that connects the extracellular ligand-binding domain to the transmembrane region.

143 olubility and stability of an active ERRbeta ligand-binding domain, thereby providing a protein tool

144 ous conformations of the estrogen receptor's ligand-binding domain, which in turn produces differenti

145 on based on the crystal structures of the MR ligand-binding domain.

146 mational change in a loop on the side of the ligand-binding-domain dimer, which leads to the formatio

147 n function and involves rearrangement of the ligand binding domains (LBDs).

148 n, suggesting uncoupling of the pore and the ligand binding domains.

149 aised by the presence of large extracellular ligand-binding domains (LBDs) and constitutive homo/hete

150 galactomannoprotein antigen with two tandem ligand-binding domains (Mp1p-LBD1 and Mp1p-LBD2), was fo

151 We solve crystal structures of LasR ligand-binding domains complexed with noncognate autoind

152 drives these conformations, we decoupled the ligand-binding domains from specific transmembrane segme

153 Inserted (I) domains function as ligand-binding domains in adhesins that support cell adh

154 , where these domains frequently function as ligand-binding domains.

155 ry effects of ligand strain energy and metal-ligand binding energy that contribute to this conformati

156 ns in supertertiary structures can shape the ligand-binding energy landscape and modulate protein-pro

157 It follows that ligand binding enhances the mechanical resistance and th

158 tree built on the similarities of predicted ligand-binding ensembles, suggesting a novel use of MAGE

159 ifying chemical substituent contributions to ligand-binding free energies is challenging due to nonad

160 information that could help distinguish CB2 ligand binding from CB1, these structures support the ex

161 many receptors that trigger endocytosis upon ligand binding, HER2 is an internalization-resistant rec

162 active, higher-affinity conformation of the ligand-binding I/A-domain (CD11b alphaA-domain).

163 Conformational changes upon ligand binding illuminate a mechanistic rationale for un

164 tailed kinetic and thermodynamic analyses of ligand binding in a heterogeneous system.

165 We also quantified OXTR ligand binding in mice deficient in Magel2, a PWS gene,

166 We found that peripheral OXTR ligand binding in the head is mostly intact in Magel2-de

167 ions can be used to describe the behavior of ligand binding in the MFED and the kinetic rate constant

168 The structural determinants of ligand binding in the prequeuosine (7-aminomethyl-7-deaz

169 Understanding how ligand binding influences protein flexibility is importa

170 f the surface area of neuraminidase, and the ligand binding inhibition is derived from glycans steric

171 is approach is based on our new finding that ligand binding inhibits aptamer digestion by T5 exonucle

172 l that the likely basis for this promiscuous ligand binding is intrinsic structural adaptability enco

173 or, which is also the first study to measure ligand binding kinetics and negative allosteric modulati

174 ctory data to form a coherent picture of the ligand binding landscape.

175 alized structural perturbations in PiuA upon ligand binding, largely consistent with recent descripti

176 differences from the extant human CYP1B1 in ligand binding, metabolism, and potential molecular cont

177 ptake receptor containing 27 CUB domains for ligand binding.METHODSWe used next-generation sequencing

178 The structure reveals a unique ligand binding mode for the K1-cluster involving cystein

179 ith diflunisal revealed a previously unknown ligand-binding mode and was consistent with the results

180 The ligand-binding mode revealed here provides a new directi

181 is series from HTS was supported by a GLP-1R ligand binding model.

182 However, the ligand-binding modes of FPR2 remain elusive, thereby lim

183 Ligand binding occurs via an induced-fit mechanism, i.e.

184 MD simulations to investigate the effect of ligand binding on the energy landscape of acyl-coenzyme

185 te the complex interplay between glycans and ligand binding on the influenza membrane protein neurami

186 rter positions to characterize the effect of ligand binding on the local structure of the orthosteric

187 ften crucial for function yet the effects of ligand binding on the mechanical stability and energy la

188 ve functional properties such as cooperative ligand binding or allosteric regulation(3).

189 the dynamics of structures in the context of ligand binding or assembly.

190 Protein activity is often regulated by ligand binding or by post-translational modifications su

191 bond activation process in the Mn/Fe R2-like ligand-binding oxidase (R2lox) protein is investigated u

192 hat a methionine and a lysine residue in the ligand binding pocket (GluN2D-Met763/Lys766, GluN2C-Met7

193 im molecules are observed within the defined ligand binding pocket and likely underlie the high poten

194 Structure-level analysis showed ligand binding pocket architectures differences in size,

195 VIP1R with its N-terminus inserting into the ligand binding pocket at the transmembrane bundle of the

196 xsA does not bind ExsD through the canonical ligand binding pocket of AraC-type proteins.

197 -terminus region and binding at the putative ligand binding pocket of CypD.

198 tion of numerous structural hot spots in the ligand binding pocket of Epa proteins is a main driver o

199 vealed their unique orientations in the SHBG ligand-binding pocket and suggested opportunities for th

200 f distinct connecting mechanisms between the ligand-binding pocket and the G-protein-binding site in

201 rearrangements not only at the bottom of the ligand-binding pocket but also in a key polar network in

202 r study suggests the existence of an optimal ligand-binding pocket conformation for capsaicin-mediate

203 ve conformation and occupies the orthosteric ligand-binding pocket enabled by a conformational change

204 r, molecular interactions that stabilize the ligand-binding pocket in its permissive conformation, an

205 ly different binding poses but stabilize the ligand-binding pocket in nearly identical permissive con

206 n, and how many permissive conformations the ligand-binding pocket may adopt, remain unclear.

207 l simulations resulted in a water accessible ligand-binding pocket that lacked sodium ions.

208 different set of key residues connecting the ligand-binding pocket to the G(s)-coupling site, and a s

209 tracellular loop (ECL) 2, which composes the ligand-binding pocket, was substantially different from

210 Arising from an in silico screen of the MR1 ligand-binding pocket, we identify one ligand, 3-([2,6-d

211 her monomer and with the DHT molecule in the ligand-binding pocket.

212 pulate the architecture of the extracellular ligand-binding pocket.

213 s an active conformation and exhibits a deep ligand-binding pocket.

214 with an architecture similar to those of the ligand-binding pockets of coronavirus hemagglutinin este

215 fields, are inherently suitable to classify ligand-binding pockets.

216 cavities within protein structures, known as ligand-binding pockets.

217 lipid environment assessed the stability of ligand-binding poses and drug-target interactions over t

218 Recent work reveals unexpected ligand binding profiles for these newly identified gluta

219 bles accurate and efficient screening of the ligand-binding profiles of individual aptamers, as well

220 ts, and whether these variants have distinct ligand-binding properties is unknown.

221 the PLEKHA7-PDZD11 interaction modulate the ligand-binding properties of PLEKHA7.

222 Many studies have analyzed the ligand-binding properties of riboswitches, but this work

223 Here, to gain new insights into the ligand-binding properties of Siglec-7, we carried out in

224 maging reveals aberrant recycling of the WNT ligand-binding protein WLS and mis-trafficking to the ly

225 r) and the protein-, nucleic acid- and small ligand-binding proteins (to study the cross-predictions)

226 Upon ligand binding, Ptch1 is removed from cilia, and Smo is

227 ements between transmembrane helices control ligand binding, receptor activation, and effector coupli

228 lass C GPCR features impact the processes of ligand binding, receptor activation, signal transduction

229 g R67) in addition to the well-known primary ligand-binding region (site 1 containing R124).

230 During animal development, ligand binding releases the intracellular domain of LIN-

231 For MN4, ligand-binding results in the reduction of dynamics that

232 CPSFL1 gene encodes a CRAL-TRIO hydrophobic ligand-binding (Sec14) domain protein.

233 functional studies, but the determinants of ligand binding, selectivity, and signaling are still poo

234 for PAR4 activation and the location of its ligand binding site (LBS) are unknown.

235 Mutations around the ligand binding site allowed salivary gland invasion but

236 findings provide a structural basis for how ligand binding site alterations can allosterically affec

237 ein originating in the peripheral C-terminal ligand binding site and culminating in pore opening.

238 Our generalizable de novo ligand binding site design method provides a foundation

239 Atomic mutation of the RNA at the ligand binding site leads to loss of binding shown by is

240 .38)A that propagated from the extracellular ligand binding site to the intracellular surface, result

241 d surface areas of atom types in the protein-ligand binding site.

242 ir conformational flexibility, together with ligand-binding site and interaction mechanisms.

243 These motifs define the ligand-binding site and make up the most structurally di

244 e via an allosteric network encompassing the ligand-binding site and the G protein-binding site.

245 We find that the base pairs close to the ligand-binding site become stronger upon ligand binding,

246 to describe how structural changes from the ligand-binding site can be transmitted to the central io

247 The extracellular ligand-binding site of DRD2 is remodelled in response to

248 hat, despite conservation of the orthosteric ligand-binding site residues, there are notable conforma

249 sidue centric and scalable method to predict ligand-binding site residues.

250 We also discover a distant (25 angstrom) ligand-binding site unique to SARS-CoV-2, which can alte

251 s information on the chemical structure, the ligand-binding site, the direction of modulation, the po

252 or MN19 decreasing in both stem 1 and at the ligand-binding site.

253 ct binding mode in the orthosteric PPARgamma ligand-binding site.

254 regulated in this way often contain multiple ligand binding sites or modification sites, which can op

255 Our results suggest that the two ligand-binding sites are potentially controlled by each

256 Detecting protein-ligand-binding sites experimentally is time-consuming an

257 perpendicularly to the NBDs and its putative ligand-binding sites face the transporter to likely modu

258 Fast and accurate classification of ligand-binding sites in proteins with respect to the cla

259 The discovery of protein-ligand-binding sites is a major step for elucidating pro

260 first transforms the molecular structures of ligand-binding sites to 2D Voronoi diagrams, which are t

261 to coordinate allosteric coupling of the two ligand-binding sites.

262 ragment subpockets and compare them to whole ligand-binding sites.

263 These calculations identified two potential ligand-binding sites.

264 upon purification, and possess deeply buried ligand-binding sites.

265 IL-11Ralpha are generally distal to putative ligand-binding sites.

266 To relate TSPO protein expression to ligand binding, specific binding of the TSPO ligands 3H-

267 Here we report the ligand binding specificity and physiological relevance o

268 he variable loop CBL2 is key for programming ligand binding specificity, albeit with limited predicta

269 uidance and shed light on Robo family member ligand binding specificity, conformational variability,

270 ot spots in conferring host cell binding and ligand binding specificity.

271 mics, and thermodynamics combine to modulate ligand-binding specificity and have implications for the

272 Our structures define the basis of ligand-binding specificity in the CRF receptor-hormone s

273 ciating compounds from real-time fluorescent ligand-binding studies.

274 structure-guided mutagenesis of RGMs and BMP ligands, binding studies, and cellular assays suggest th

275 The ACE competes with ligand binding, such that DAs generate a signal upon lig

276 Cs respond faster and more vigorously to TLR ligand binding than their closely related macrophages.

277 anges within the soluble protein calmodulin, ligand binding to a G protein-coupled receptor, and acti

278 In rare instances, ligand binding to a riboswitch has been found to alter t

279 nd states, the structural mechanism coupling ligand binding to channel gating is unknown.

280 smitter binding site, but modify coupling of ligand binding to channel opening.

281 tence of innate immune receptors that couple ligand binding to endocytic vesicle damage to permit MHC

282 abotropic NMDAR pathway (i.e., by preventing ligand binding to NMDARs with competitive antagonists or

283 onical pathway involving first activation of ligand binding to Patched followed by alleviation of Smo

284 aracterized here, allosterically couples the ligand binding to S1 through a similar mechanism.

285 amine in S1 is allosterically coupled to the ligand binding to S2 through altering protein conformati

286 Previous molecular dynamics studies on ligand binding to the beta(2)-adrenergic receptor (beta(

287 AG channel gating that could be activated by ligand binding to the PAS domain.

288 TRPV1 S1-S4 membrane domain couples chemical ligand binding to the pore domain during channel gating.

289 a two- to eightfold decrease in the rate of ligand binding to the primary binding site of neuraminid

290 gating changes in the capacitance induced by ligand binding to the serotonin transporter and to the g

291 a result of the high surface-charge density, ligand binding to this protein is allosterically activat

292 Ligand binding to thrombin takes place exclusively in th

293 identified small molecules that are genuine ligands binding to the RET extracellular domain.

294 rmine hH(3,4)Rs/mH(4)R binding affinities of ligands binding to these receptors.

295 udies indicate that substrate and inhibitor (ligands) binding to ABCG2 can be differentiated quantita

296 The ligand binding transition states predicted by these Mark

297 ce on a C57BL/6J background, specific AVPR1A ligand binding was observed in the neonatal mouse periph

298 the ligand-binding site become stronger upon ligand binding, whereas those located away from the bind

299 apable of directly detecting and quantifying ligand binding within a wide range of membrane protein s

300 ucidate allostery at atomic resoluion on the ligand-binding WW domain of the enzyme Pin1, multistate