ライフサイエンスコーパス: limited proteolysis

1 gen and for heparin and by susceptibility to limited proteolysis.

2 through transmission electron microscopy or limited proteolysis.

3 negatively modulated by proteasome-mediated limited proteolysis.

4 and bell-shaped peak intensity profiles, and limited proteolysis.

5 dant local conformational change detected by limited proteolysis.

6 n folding, thermostability, and tolerance to limited proteolysis.

7 and high structural flexibility as probed by limited proteolysis.

8 truncated protein was slightly diminished by limited proteolysis.

9 nal modifications and regions susceptible to limited proteolysis.

10 rminates at a domain boundary, as defined by limited proteolysis.

11 ses confirmed that HP21 activates proPAP3 by limited proteolysis.

12 ivating procoagulant factors Va and VIIIa by limited proteolysis.

13 ein in the presence and absence of DNA using limited proteolysis.

14 en defined the minimal structured core using limited proteolysis.

15 ntification of a putative domain of xNAP1 by limited proteolysis.

16 footprinting, fluorescence spectroscopy, and limited proteolysis.

17 ations of Hop and Hop mutants were probed by limited proteolysis.

18 scade by degrading cofactors Va and VIIIa by limited proteolysis.

19 Similar folded regions were detected by limited proteolysis.

20 , P81L and V126D, on the structure of p16 by limited proteolysis.

21 g, and enhance the A1 domain's resistance to limited proteolysis.

22 Domain architecture was further studied by limited proteolysis.

23 ts and a structural perturbation revealed by limited proteolysis.

24 ic misfolding of GGPP as studied by in vitro limited proteolysis.

25 r52Pro TTR was generated readily in vitro by limited proteolysis.

26 rsors, or zymogens, that become activated by limited proteolysis.

27 terminal ends of fibronectin, we prepared by limited proteolysis a dimeric 140 kDa (Fib-2)2 fragment

28 ed N-terminal region of IL-1ra is removed by limited proteolysis, a 2-fold increase in the time cours

29 ng of the loop conformation as elucidated by limited proteolysis, accompanied by the impaired binding

30 Limited proteolysis, accomplished by endopeptidases, is

31 actin (Ybt) gene cluster of Yersinia pestis, limited proteolysis afforded a key 11 kDa peptide from t

32 Limited proteolysis also reveals that phosphorylation in

33 Circular dichroism and limited proteolysis analysis indicate that magnesium ind

34 ing nucleotide analogue could be detected by limited proteolysis analysis, a comparison of the stabil

35 Here, through limited proteolysis analysis, we demonstrate that the ev

36 The purified N-terminal domain prepared by limited proteolysis and anion exchange chromatography wa

37 shadowing electron microscopy, combined with limited proteolysis and antibody labeling, demonstrates

38 Limited proteolysis and biochemical experiments indicate

39 Limited proteolysis and chemical cross-linking suggest u

40 Here we used time-resolved limited proteolysis and chemical cross-linking to examin

41 Previous work using limited proteolysis and chemical modification revealed t

42 Limited proteolysis and deletion analyses show that both

43 We have used limited proteolysis and deletion analysis to probe the c

44 Using limited proteolysis and deletion mutagenesis we show tha

45 etence in diabetes; this was confirmed using limited proteolysis and denaturation/refolding.

46 Small-angle X-ray scattering, limited proteolysis and differential scanning fluorimetr

47 However, limited proteolysis and electron microscopy observations

48 ytoplasmic domain of EpsL was identified via limited proteolysis and facilitated the crystallization

49 tting and then examined the mechanisms using limited proteolysis and fluorescence resonance energy tr

50 the intron-maturase complex, and results of limited proteolysis and fluorescence spectroscopy experi

51 Here, we have harnessed the power of limited proteolysis and heterologous protein expression

52 The combination of limited proteolysis and MALDI-TOF mass spectrometry has

53 tion in Ku structure, which was confirmed by limited proteolysis and mass spectrometry analyses.

54 Using limited proteolysis and mass spectrometry, we found that

55 Using limited proteolysis and mass spectrometry, we show that

56 most of the PRLR ECD, a result confirmed by limited proteolysis and mass spectrometry.

57 esonance (SPR) and an approach that combines limited proteolysis and mass spectrometry.

58 Domain mapping of the 98-kDa enzyme by limited proteolysis and NaBH(4) cross-linking with a BER

59 Limited proteolysis and peptide mass mapping of intact p

60 This was confirmed by limited proteolysis and protein sequencing, which showed

61 n partially inhibited, proteasomes carry out limited proteolysis and release the processed Nrf1 (lack

62 Limited proteolysis and REMSA demonstrate the accessibil

63 kB coiled coil, obtained by a combination of limited proteolysis and site-directed cross-linking appr

64 Differential scanning calorimetry, limited proteolysis and small-angle X-ray scattering (SA

65 Limited proteolysis and surface-topology mapping of prot

66 We confirmed these findings using limited proteolysis and tryptophan fluorescence experime

67 Each of these toxins undergoes limited proteolysis and/or disulfide bond reduction to f

68 kened zinc binding, increased sensitivity to limited proteolysis, and altered circular dichroism spec

69 tryptophan fluorescence, circular dichroism, limited proteolysis, and cross-linking.

70 erscored by the results of alanine-scanning, limited proteolysis, and deletion analysis, which show t

71 mpetition assays, cross-linking experiments, limited proteolysis, and mass spectrometry revealed that

72 We used immunoblotting, limited proteolysis, and mass spectrometry to localize g

73 In addition, via chemical cross-linking, limited proteolysis, and mass spectrometry, we identifie

74 Infectivity, limited proteolysis, and matrix-assisted laser desorptio

75 Intrinsic fluorescence, limited proteolysis, and molecular dynamics studies sugg

76 imensional nuclear magnetic resonance (NMR), limited proteolysis, and small-angle X-ray scattering (S

77 orbance and circular dichroism spectroscopy, limited proteolysis, and solution NMR.

78 s of CBS indicate that activation by AdoMet, limited proteolysis, and thermal denaturation share a co

79 y Ca(2+), as shown by Cibacron blue binding, limited proteolysis, and tryptophan fluorescence.

80 ctured core, revealed by Raman spectroscopy, limited-proteolysis, and fibril disaggregation experimen

81 Intracellular peptides generated by limited proteolysis are likely to function inside and ou

82 We devised an in vitro limited proteolysis assay of Hmg2p in its native membran

83 elevance of this model behavior, we used the limited proteolysis assay to examine the effects of chan

84 Limited proteolysis assays indicate that acetylation of

85 nucleotide binding, kinase measurements, and limited proteolysis assays on this semisynthetic ATP-lin

86 We developed limited proteolysis assays using trypsin and V8 protease

87 Limited proteolysis assays using trypsin in the absence

88 Using limited proteolysis assays, nucleotide-binding assays, a

89 determined by UIC2 antibody shift assays and limited proteolysis assays.

90 polymerization of the large multimers is the limited proteolysis by a vWF-cleaving protease present i

91 istently, DBD-B became more resistant to the limited proteolysis by chymotrypsin after RPA hyperphosp

92 After limited proteolysis by proteinase K, the most abundant f

93 m assumes that FV activation is initiated by limited proteolysis by traces of (meizo) thrombin.

94 and studied changes in Tm susceptibility to limited proteolysis by trypsin and changes in regulation

95 Limited proteolysis by trypsin results in the production

96 Limited proteolysis by trypsin shows that binding of nuc

97 drogen-deuterium exchange mass spectrometry, limited proteolysis, circular dichroism spectroscopy, an

98 Limited proteolysis, circular dichroism, and fluorescenc

99 TRCF mutants are dramatically sensitized to limited proteolysis compared with repressed TRCF, pointi

100 Analysis by limited proteolysis confirmed the identity of Gbeta(5) i

101 Limited proteolysis confirms assembly of stargazin with

102 he isoforms, bRPGRIP1 and bRPGRIP1b, undergo limited proteolysis constitutively in vivo in the cytopl

103 Limited proteolysis coupled with LC-MS/MS could reveal t

104 Using limited proteolysis coupled with mass spectrometric anal

105 fied experimentally, with techniques such as limited proteolysis coupled with mass spectrometry, or c

106 ering, high-resolution NMR spectroscopy, and limited proteolysis coupled with mass spectrometry, we s

107 eosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spec

108 lity of the domain 3-4 linker, inferred from limited proteolysis data and from the relaxation behavio

109 Limited proteolysis data conducted on the MA-CaM complex

110 NMR and limited proteolysis data suggest that approximately 30 a

111 This shape, together with limited proteolysis data, suggests that the S1-RNA bindi

112 Limited proteolysis defined an independently folded C-te

113 Binding of the hydrophobic probe bis-ANS and limited proteolysis demonstrate CII proteins undergo sig

114 Limited proteolysis demonstrates that a structural core

115 as the minimal coiled-coil domain defined by limited proteolysis, did not retain the ability to inter

116 was assayed by biochemical methods including limited proteolysis, dynamic light scattering, CD, and N

117 Limited proteolysis experiments combined with mass spect

118 Cosedimentation and limited proteolysis experiments indicated a similar acti

119 Limited proteolysis experiments reveal redox- and mutati

120 Conversely, limited proteolysis experiments show a reduced protease

121 In addition, limited proteolysis experiments showed a reduced stabili

122 This conclusion is supported by limited proteolysis experiments showing an increase in t

123 Sedimentation velocity and limited proteolysis experiments suggest that heme bindin

124 ns disrupt the STAS domain directly, whereas limited proteolysis experiments suggest that the DeltaY5

125 Limited proteolysis experiments suggest the optimal reac

126 These observations are confirmed by limited proteolysis experiments that reveal an intrinsic

127 DnaJ using in vitro cross-linking assays and limited proteolysis experiments that show that this inte

128 Also, by systematic truncation and limited proteolysis experiments we identified a portion

129 Limited proteolysis experiments with epitope-tagged copi

130 Circular dichroism and limited proteolysis experiments with F-virosomes indicat

131 ulated ATPase activity and with results from limited proteolysis experiments, which indicated that th

132 In this report, we have used limited proteolysis, filter binding, and NMR spectroscop

133 nsoluble SOD1 amyloid fibrils, we first used limited proteolysis followed by mass spectrometric analy

134 thione S-transferase (GST) pull-down assays, limited proteolysis followed by mass spectrometry, and c

135 62 StAR but were structured as determined by limited proteolysis followed by mass spectrometry.

136 We found limited proteolysis followed by RP-HPLC-MS to be less ti

137 ening, and autoinactivation, consistent with limited proteolysis fragmentation patterns.

138 Limited proteolysis further defines regions of primary s

139 Limited proteolysis, hydrodynamic, and kinetic measureme

140 However, limited proteolysis identifies a sequence flanking the C

141 buted, calcium-dependent enzymes that induce limited proteolysis in a wide range of substrates.

142 te antigens (HLA) bind peptides generated by limited proteolysis in cells and present them at the cel

143 present work has demonstrated the utility of limited proteolysis in helping to elucidate the potentia

144 on of GC, fluorescence emission spectra, and limited proteolysis in the absence and presence of Ca2+.

145 l structures, whereas spectroscopic data and limited proteolysis indicate that H6 displays poorly coo

146 Activity is generated following limited proteolysis indicating that the conversion of fa

147 These results suggest that limited proteolysis is another post-translational mechan

148 Cleavage pattern of SMC2 by limited proteolysis is changed upon its binding to ATP o

149 protein fragments in vivo, whereas in vitro limited proteolysis is often employed to probe different

150 we show that a C-terminal domain isolated by limited proteolysis is tetrameric in solution, like the

151 Using circular dichroism spectroscopy, limited proteolysis, kinase assays, and isothermal titra

152 Limited proteolysis, kinetic simulations, and MS analyse

153 We use an array of approaches (limited proteolysis, magic angle spinning NMR, Fourier t

154 Limited proteolysis, mass spectrometry and deletion anal

155 Limited proteolysis, mass spectrometry and N-terminal se

156 nitrificans were supported by cross-linking, limited proteolysis, mass spectrometry, and functional d

157 For this study, we use a combination of limited proteolysis, mass spectrometry, and N-terminal s

158 We used surface plasmon resonance, limited proteolysis, mass spectrometry, and NMR spectros

159 protein to the postfusion conformation using limited proteolysis, mass spectrometry, and single-parti

160 To test this hypothesis, limited proteolysis, mass spectrometry, and solution-sta

161 y from the current model and correlates with limited proteolysis, matrix-assisted laser desorption/io

162 Limited proteolysis measurements confirm the expected do

163 uncated form is generated intracellularly by limited proteolysis mediated by proprotein convertase(s)

164 high-throughput alternative to the classical limited proteolysis method for the isolation of stable c

165 Limited proteolysis, microsequencing, and sedimentation

166 g a combination of top-down and middle-down (limited proteolysis) MS approach, which ensures full seq

167 Limited proteolysis, NMR spectroscopy and chromophoric c

168 s approach, we also performed membrane-based limited proteolysis of a large Arabidopsis GTPase, Root

169 Presenilins have been shown to influence limited proteolysis of amyloid beta protein precursor (A

170 Limited proteolysis of an N-terminal construct from resi

171 Here we test the validity of this model by limited proteolysis of B17 and the characterization of i

172 ells with anti-Fas induced caspase-dependent limited proteolysis of beta-catenin N- and C-terminal re

173 co-regulates the metalloproteinase-mediated limited proteolysis of beta1AR.

174 Limited proteolysis of betaB1 produced betaB1DeltaN47, w

175 Limited proteolysis of BjPutA under reducing conditions

176 Limited proteolysis of BPL with trypsin and chymotrypsin

177 Limited proteolysis of BRCA1/BARD1 complexes, monitored

178 ted by a specific stimulus, caspases execute limited proteolysis of downstream substrates to trigger

179 The structure of the T.acidophilum LplA and limited proteolysis of E.coli LplA together highlighted

180 Limited proteolysis of epitope-tagged c" (Vma16p) indica

181 FV as an inactive procofactor and reveal how limited proteolysis of FV progressively destabilizes key

182 domain in Gis1 and is essential for both the limited proteolysis of Gis1 and its full activity.

183 ied several different aggregating sites from limited proteolysis of harvested aggregates and effects

184 e use for these purposes is the induction of limited proteolysis of host proteins, which is the main

185 We have defined by limited proteolysis of human capping enzyme residues 229

186 Limited proteolysis of IPK1 revealed that N-lobe stabili

187 Limited proteolysis of Lef4 and ensuing COOH-terminal de

188 Employing the method of limited proteolysis of lipid droplet surface proteins, w

189 The technique is based on limited proteolysis of live cells in the absence of dena

190 Limited proteolysis of MAB007 with Lys-C led to a single

191 Limited proteolysis of native and recombinant Cdc37, in

192 solated the N-terminal domain in vitro using limited proteolysis of native OCP.

193 in of approximately 15 kDa was detected upon limited proteolysis of oxidized but not reduced Yap1p.

194 as a protease involved in the degradation or limited proteolysis of parasite proteins involved in inv

195 ere reduced by the PfKelch13 mutation, which limited proteolysis of PfPI3K and thus increased levels

196 Limited proteolysis of PR-B demonstrated that the hydrod

197 uding PC3 (also known as PC1), that catalyze limited proteolysis of precursor proteins.

198 the appearance of a 119-kDa fragment during limited proteolysis of proline-reduced PutA.

199 Limited proteolysis of purified S. aureus DNA ligase by

200 Previously, limited proteolysis of PutA indicated that the conformat

201 einforces the biofilm structure and leads to limited proteolysis of RbmA to RbmA*.

202 Limited proteolysis of RNase-A yields a short N-terminal

203 s, which induce neuronal death by catalyzing limited proteolysis of specific cellular proteins.

204 Limited proteolysis of spheroplasts revealed that the C-

205 a(2+)-activated proteases that result in the limited proteolysis of substrate proteins and subsequent

206 We demonstrated previously that limited proteolysis of talin1 by the calcium-dependent p

207 Limited proteolysis of TbCet1 suggests that the hydrophi

208 competes with FXa for binding to FV(a), and limited proteolysis of the B-domain, specifically at Arg

209 Full or limited proteolysis of the collagen fibril is known to b

210 or the monovalent Fab fragments derived from limited proteolysis of the covalently modified 8A11 were

211 We show that limited proteolysis of the detergent-solubilized and pur

212 Limited proteolysis of the Escherichia coli cell divisio

213 Although limited proteolysis of the histone H3 N-terminal tail (H

214 We have used limited proteolysis of the in vitro-translated mouse AhR

215 Limited proteolysis of the NLD revealed characteristic f

216 Limited proteolysis of the pyruvate decarboxylase (E1, a

217 Limited proteolysis of the recombinant A6 protein identi

218 we identified a putative hrg binding site by limited proteolysis of the recombinant extracellular dom

219 ajor structural transformation, triggered by limited proteolysis of the scaffolding proteins.

220 ent from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain.

221 Circular dichroism spectroscopy and limited proteolysis of these reconstituted particles ind

222 Consistent with this proposal, limited proteolysis of wild-type and hybrid modules show

223 sucrose gradient centrifugation experiments, limited proteolysis, one-dimensional NMR, and beta-lacta

224 rgoing conformational alterations induced by limited proteolysis or elevated temperature.

225 ormed into a potent antiangiogenic factor by limited proteolysis or mild heating.

226 ition to circular dichroism spectroscopy and limited proteolysis, our strategy incorporates nanogold-

227 milar with regard to cellular concentration, limited proteolysis patterns, rate of autophosphorylatio

228 erized the microtubule-binding properties of limited proteolysis products and subcomplexes of DASH, t

229 -ray scattering (SAXS) data-based models and limited proteolysis profiles of some IgG1 mAbs known to

230 Three approaches, ATP hydrolysis assays, limited proteolysis protection, and equilibrium dialysis

231 Finally, limited proteolysis provided direct evidence that the pr

232 Limited proteolysis rapidly degrades MPs to a stable C-t

233 f exposed sites on the fibril as a result of limited proteolysis, regulate these processes and that o

234 d alpha-synuclein very efficiently, and that limited proteolysis resulted in the generation of carbox

235 Experiments on limited proteolysis reveal that a long range conformatio

236 Limited proteolysis revealed that the linker between the

237 Limited proteolysis reveals a bipartite organization con

238 Limited proteolysis reveals that eIF3 binding to the 40S

239 pproach, well-established techniques such as limited proteolysis, reversed-phase (RP) high-performanc

240 Limited proteolysis separates ER from mitochondria, wher

241 Structural analysis and limited proteolysis show that sterol binding closes the

242 Structure probing by limited proteolysis showed that AmEPV ligase is punctuat

243 ourier transformed infrared spectroscopy and limited proteolysis showed that fibrillar PABPN1 has a s

244 Although HspBP1 is encoded by seven exons, limited proteolysis shows that it has only two structura

245 Limited proteolysis, small-angle X-ray scattering, and f

246 Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fra

247 Limited proteolysis studies of MutLalpha demonstrated th

248 we used trypsin and chymotrypsin to conduct limited proteolysis studies on synthetic amyloid fibrils

249 Limited proteolysis studies suggest that PR-A asymmetry

250 Limited proteolysis studies suggested that the R445L mut

251 sis on Asp134, and chemical modification and limited proteolysis studies, we propose a catalytic mech

252 Sequence comparisons and limited proteolysis suggest that their folds are conserv

253 Limited proteolysis suggested further conformational dif

254 Limited proteolysis supported a structural role for this

255 , but when partially inhibited, they perform limited proteolysis that generates the active form of Nr

256 ay ionisation mass spectrometry (ESI-MS) and limited proteolysis that homocysteine attacks intramolec

257 Using cross-linking and limited proteolysis, the alpha-gamma intersubunit contac

258 DSDO rendered the kinase very susceptible to limited proteolysis; the former preferentially caused th

259 amide peptide using experimental techniques (limited proteolysis, thermal denaturation, urea denatura

260 We have used NMR spectroscopy and limited proteolysis to characterize the structural prope

261 loped a method to produce NS5A and performed limited proteolysis to confirm the domain organization m

262 Here we apply limited proteolysis to cystatin B amyloid fibrils and sh

263 the disease-causing PrP isoform (PrPSc) used limited proteolysis to digest the precursor cellular PrP

264 scence spectroscopy, molecular modeling, and limited proteolysis to examine structural dynamics of th

265 We used NMR spectroscopy together with limited proteolysis to examine the dynamics of the lambd

266 We have used limited proteolysis to generate a fragment of RGS9-1 tha

267 events in the self-assembly of IAPP, we used limited proteolysis to identify an exposed and flexible

268 on NMR spectroscopy, circular dichroism, and limited proteolysis to investigate the effects of these

269 Here, we use limited proteolysis to investigate the role of the N and

270 terium exchange-mass spectrometry (DXMS) and limited proteolysis to probe the folded regions of D-AKA

271 tionally encoded, we used amide exchange and limited proteolysis to probe the structures of these two

272 st prion protein (PrP), which is assessed by limited proteolysis to yield resistant PrP bands (PrP-re

273 The 88-kDa secreted VacA protein can undergo limited proteolysis to yield two domains, designated p33

274 uctural analyses of tRNase Z(L) performed by limited proteolysis, two dimensional gel electrophoresis

275 Applying limited proteolysis using endoproteinase Lys-C resulted

276 The cysteinylation observed after limited proteolysis was confirmed by peptide mapping cou

277 Limited proteolysis was used to identify a stable and fu

278 Limited proteolysis was used to locate involvement in th

279 Using limited proteolysis we have also shown that HspBP1 assoc

280 Using limited proteolysis, we also identified modifications re

281 Using NMR spectroscopy and limited proteolysis, we demonstrate that several of thes

282 Using in situ limited proteolysis, we demonstrated a clear change in t

283 Here, using limited proteolysis, we map the site of disorder in unli

284 Sequence alignment and limited proteolysis were used to identify and eliminate

285 of dysferlin, we have applied the method of limited proteolysis, which allows nonspecific digestion

286 o interdomain boundaries were identified via limited proteolysis, which led to the production of a 90

287 The p48/p58 dimer was subjected to limited proteolysis, which produced two stable structura

288 e titration, tryptophan emission spectra, or limited proteolysis, which suggests that the E240K mutat

289 features of prothrombin can be monitored by limited proteolysis with chymotrypsin that attacks W468

290 The limited proteolysis with Glu-C or Lys-C proteases shows

291 Limited proteolysis with MMP-2 and -9 degraded PEDF in a

292 Limited proteolysis with pepsin was used to determine se

293 Limited proteolysis with trypsin and V8 proteases was us

294 Limited proteolysis with trypsin was employed to demonst

295 lubilized from porcine thyroid microsomes by limited proteolysis with trypsin.

296 dney) or endothelial (human brain) cells, by limited proteolysis with trypsin.

297 mains, with generation of activity requiring limited proteolysis within a region that separates the l

298 Here, we show that RbmA undergoes limited proteolysis within the biofilm.

299 ross-link bond between actin protomers using limited proteolysis, X-ray crystallography, and mass spe

300 type IX collagen (CIX) from cartilage using limited proteolysis yielded partially degraded material.