ライフサイエンスコーパス: living cell

1 sugars into the cell surface glycome of the living cell.

2 most exclusively, outside the context of the living cell.

3 vironment of a giant vesicle, derived from a living cell.

4 ng from a simple phenomenological model of a living cell.

5 s can influence the cofactor repertoire of a living cell.

6 drastically reduces such specificity in the living cell.

7 h size and count molecules for vesicles in a living cell.

8 interaction of mitochondria and lysosomes in living cells.

9 pping, in molecular detail, processes inside living cells.

10 ymerase 1, an important anticancer target in living cells.

11 circuits allow us to modify the behavior of living cells.

12 lexed imaging of miRNAs cancer biomarkers in living cells.

13 de identification of GAG-binding proteins in living cells.

14 enous conformers during agonist treatment in living cells.

15 nt turn-on probe for labeling sphingosine in living cells.

16 and distribution of an alkyne-based drug in living cells.

17 leic acid recognition element to probe pH in living cells.

18 ive detection of low-abundance biomarkers in living cells.

19 ted strategies for bioorthogonal labeling in living cells.

20 n genes has never been addressed directly in living cells.

21 to assay Ca(2+) and calcineurin activity in living cells.

22 ation from individual HIV-1 RNA molecules in living cells.

23 ational regulatory elements that function in living cells.

24 e the subcellular location of RNA targets in living cells.

25 of these high energy light sources to damage living cells.

26 little is known about chromatin dynamics in living cells.

27 or quantitatively studying lipid function in living cells.

28 nt biosensor probes simultaneously in single living cells.

29 ced by chemically distinct opioid ligands in living cells.

30 ody) for ligand-gated antigen recognition in living cells.

31 iate transport states of Lipid II on MurJ in living cells.

32 hanical properties of isolated nuclei and in living cells.

33 behavior at mesoscales both in vitro and in living cells.

34 er-resolution imaging of membrane tension in living cells.

35 on and instantaneous fluorescence imaging in living cells.

36 a lack of tools for detecting sphingosine in living cells.

37 is the most abundant divalent cation in all living cells.

38 ds to successfully image low-abundance TR in living cells.

39 protein (APP) along the endocytic pathway of living cells.

40 stems for monitoring flavivirus infection in living cells.

41 f activation-relaxation properties in intact living cells.

42 kinetics often observed from fluorophores in living cells.

43 and interrogation of MCSs in both fixed and living cells.

44 e "recognition unit" (ligand) for F-actin in living cells.

45 old nanoparticles in the endolysosome of the living cells.

46 alyses of transient RNA processing events in living cells.

47 o enable this control for PLCbeta enzymes in living cells.

48 estigate the interaction between proteins in living cells.

49 ological processes without the use of intact living cells.

50 other processes that affect transcription in living cells.

51 lent strategy to monitor protein dynamics in living cells.

52 scovery of novel RNA-protein interactions in living cells.

53 targeted sequence changes to the genomes of living cells.

54 processes in the nucleus, often in single or living cells.

55 cale electronic interface to the interior of living cells.

56 cs of microtubule and their PTM processes in living cells.

57 because it has been difficult to observe in living cells.

58 toolkit to study cAMP-regulated processes in living cells.

59 rly 100% of them are present in complexes in living cells.

60 new principle to explain the organization of living cells.

61 vestigation of the DNA methylation states in living cells.

62 rs have emerged to detect various targets in living cells.

63 rgoes to enhance the uptake of proteins into living cells.

64 sulfate from organic sulfur contained within living cells.

65 form a stretchable, hyperelastic network in living cells.

66 he way to first-principles modeling of whole living cells.

67 f the surrounding nucleoplasm on nucleoli in living cells.

68 ools for the detection of DNA methylation in living cells.

69 atiotemporal dynamics of protein activity in living cells.

70 cture with high spatiotemporal resolution in living cells.

71 ER tubules and sheets for the first time in living cells.

72 ical gradients across the plasma membrane of living cells.

73 that is central to protein synthesis in all living cells.

74 pply in the physiological environment inside living cells.

75 ks and organelle morphologies extracted from living cells.

76 their signal cross-talk on phagosomes inside living cells.

77 oupled receptor (GPCR) signaling pathways in living cells.

78 H(2)O(2))-mediated oxidation in vitro and in living cells.

79 d the unprecedented labeling of active AC in living cells.

80 omplexes that enable live imaging of mRNA in living cells.

81 a direct binding partner of ipomoeassin F in living cells.

82 s) during DNA replication, is challenging in living cells.

83 n vivo approach to study DVL conformation in living cells.

84 ymatic functionality of BRG1 in vitro and in living cells.

85 on of S6K1 occurs mainly in the cytoplasm of living cells.

86 cuits to perform sophisticated operations in living cells.

87 N in the cytoplasm but not in the nucleus of living cells.

88 eras and example oligomeric complexes inside living cells.

89 tructures with features similar to cables in living cells.

90 protein interaction networks on long RNAs in living cells.

91 structure inside the crowded environment of living cells.

92 s to organelle-like hydrogel architecture in living cells.

93 r G(s), G(q), and G(i) signaling pathways in living cells.

94 tional mitochondria-lysosome interactions in living cells.

95 that can be used to deliver biomolecules to living cells.

96 s across the membranes of lipid vesicles and living cells.

97 ime detection of individual G4 structures in living cells.

98 mation about the behavior of biomolecules in living cells.

99 ul means to quantify biochemical dynamics in living cells.

100 stood how mu-ORs produce specific effects in living cells.

101 tion initiation is a fundamental property of living cells.

102 g of anaphase, and we verify our findings in living cells.

103 r studying diffusion and protein dynamics in living cells.

104 atterned to crosslink alginate gels and trap living cells.

105 uch as hydrogel alginate capsules containing living cells.

106 an important organizational principle within living cells.

107 ribution of protein recovery or diffusion in living cells.

108 idence in support of dynamic G4 formation in living cells.

109 and Sad1/UNC-84 (SUN) proteins, in the NE of living cells.

110 s of flavivirus NS2B-NS3 serine proteases in living cells.

111 lify the complex biosynthetic machineries of living cells.

112 tivation and titration of gene expression in living cells.

113 tion, and relocated topoisomerase IIalpha in living cells.

114 ates using real-time kinetic measurements in living cells.

115 f each domain that drives the Tau cluster in living cells.

116 ion events and multiplexed discrimination of living cells.

117 n-mediated control of biological function in living cells.

118 he dynamics and fluxes of small molecules in living cells.

119 pheral membrane protein binding to the PM in living cells.

120 medicine through the genetic programming of living cells(1,2).

121 y a weak capacity to form liquid droplets in living cells; (2) the size, global accessibility, and co

122 t a strong tendency to phase separate within living cells, a process that can drive localized RNA tra

123 High-throughput and dynamic measurement for living cell activities can benefit biological research a

124 escent tags for visualization of proteins in living cells add six to several hundred amino acids to t

125 Photoisomerising these reagents in living cells allows optical control over microtubule net

126 ited excellent cell membrane permeability in living cells and a higher selectivity for mitochondria c

127 can be used to perturb signaling networks in living cells and animals with high spatiotemporal resolu

128 rs, FiNad, for monitoring NAD(+) dynamics in living cells and animals.

129 nvasive monitoring of DSB repair pathways in living cells and animals.

130 y towards active interfacing with individual living cells and cell communities.

131 tification of these mutations challenging in living cells and complex reaction environments.

132 olecule techniques when studying kinetics in living cells and discuss solutions to specific challenge

133 the dynamics of GFP-labeled microtubules in living cells and found that lowering temperature from 37

134 show localisation of S6K1 phosphorylation in living cells and hence a key site of action of inhibitor

135 ted the mechanisms of the BBSome assembly in living cells and how this process is spatially regulated

136 been realized in the compartmentalization of living cells and in obtaining ordered but dynamic partit

137 and studying the effects of nanoparticles in living cells and neurons.

138 esterase both in vitro and on the surface of living cells and neurons.

139 tructural probing of protein interactions in living cells and organisms.

140 ht on in-bulk myosin-driven cell motility in living cells and provide a framework to design a novel t

141 ly described a method to introduce DELs into living cells and recover conjugates that bind to an intr

142 rotocol quantifies the endocytic activity of living cells and the recruitment of associated proteins

143 in understanding macromolecular crowding in living cells and their effects on protein folding, and s

144 provides a snapshot of the dynamic state of living cells and tissue.

145 Living cells and tissues experience various complex mode

146 CL-1 can monitor dynamics of HNO delivery in living cells and tissues, demonstrating the versatility

147 understanding of how chromatin works within living cells and tissues.

148 through means that are highly disruptive to living cells and tissues.

149 microinject fluorescently-labeled tRNA into living cells and use confocal microscopy to image tRNA s

150 lied to the real-time detection of CYP3A4 in living cells and zebrafish.

151 A tracks, DNA robots performing tasks within living cells and/or DNA tweezers as ultra-sensitive bios

152 ents and molecular motors generate motion in living cells, and have internal structures ranging from

153 nvolved at many stages of the development of living cells, and often external forces applied to a bio

154 ntitative biophysical tests of this model in living cells, and phase separation has not yet been dire

155 luating the selectivity of CDK inhibitors in living cells, and present a refined set of tool molecule

156 odulation of the microtubule cytoskeleton in living cells, and promise new possibilities for studying

157 rapid and reversible binding to chromatin in living cells, and transcription occurs in sporadic burst

158 cted or metabolically traumatized, but still living, cells, and this 'murder by phagocytosis' may be

159 cal effects of circularly polarized light on living cells are considered to be negligibly weak.

160 Living cells are known to be in thermodynamically nonequ

161 er, observations of this phase transition in living cells are limited.

162 conditions, and find that regions containing living cells are maintained at cooler temperatures.

163 ing the abundance of proteins of interest in living cells are powerful tools for studying protein fun

164 e fluorometabolites into the biochemistry of living cells are scarce.

165 rene beads, drug delivery microcapsules, and living cells) are patterned in response to a brief expos

166 Synthetic biology uses living cells as the substrate for performing human-defin

167 ssing conformational changes in receptors in living cells at ambient conditions.

168 molecule imaging of mu-ORs on the surface of living cells at physiological expression levels.

169 nce resonance energy transfer experiments on living cells, biochemical and mutational analysis, we sh

170 struction of organelle-like architectures in living cells, but has proven difficult due to the lack o

171 atiotemporal regulation of cell signaling in living cells, but the utility of the red spectrum for bi

172 ular events to achieve tailored functions in living cells, but their applications to probe the struct

173 ficient to build and maintain the S-layer in living cells by efficient protein crystal nucleation and

174 easurement of local mechanical properties of living cells by nano/micro indentation relies on the fou

175 improve the efficiency and predictability of living cells by removing extraneous genes from their gen

176 cytokine receptors in the plasma membrane of living cells by single-molecule fluorescence microscopy.

177 ir utility as selective chromatin binders in living cells by stably expressing eCRs in mouse embryoni

178 sh whether proteins not normally released by living cells can be extracted without harming cells, wit

179 a focus on cytoskeletal organization in free-living cells, ciliates in particular, in which these pro

180 (3D) displacement in real time under complex living cell conditions.

181 as the most effective for GRK inhibition in living cells, confirming that GRK2 predominantly drives

182 tory of a single protein in the cytosol of a living cell contains information about its molecular int

183 The interior of a vital, living cell contains multiple dynamic and electrically a

184 Living cells control crystallization using chemical reac

185 nalysis of synthetic and natural circuits in living cells could be made more scalable using the same

186 Using LLSM to image biosensors in living cells could provide unprecedented visualization o

187 asic routes: they either aggregate from free-living cells, creating potentially chimeric multicellula

188 pe times of DNA-binding species diffusing in living cells: CRISPR-Cas9, TetR, and LacI.

189 on (STED) microscopy, we demonstrate that in living cells, CTCF forms clusters typically containing 2

190 The study of complex signaling networks in living cells demands the ability to track more than one

191 ingle-molecule study of membrane p75(NTR) in living cells, demonstrating that the vast majority of re

192 Living cells do not interface naturally with nanoscale m

193 In living cells, dynamics of the endoplasmic reticulum (ER)

194 nsight into the ancient mechanism that helps living cells ensure the stereochemistry of protein synth

195 s demonstrated by competition experiments in living cells expressing CB(2)R at native levels.

196 obility of CD56 receptors on whole fixed and living cells, finding that CD56 accumulated at cell-cell

197 The reagents were also used in living cells for cysteine proteomic profiling and displa

198 ld tumor constructs via precise placement of living cells, functional biomaterials, and programmable

199 Bioluminescence-based imaging of living cells has become an important tool in biological

200 dosomes, but controlling this process within living cells has been challenging.

201 ently, methods for assessing drug binding in living cells have advanced and are now integral to medic

202 Surprisingly, experiments in living cells highlight a role for this module in the ass

203 ase a potent kinase inhibitor, ibrutinib, in living cells, highlighting its broad utility in controll

204 membrane proteases and unrelated proteins in living cells (human and Drosophila) and planar lipid bil

205 cal signal within biochemical processes of a living cell in a human designed way and thus, may have s

206 sed to statistically quantify LD dynamics in living cells in a label-free manner.

207 stematically map RNA-protein interactions in living cells in an unbiased manner.

208 etection of lysine synthesized by only a few living cells in microfluidic droplets via mass spectrome

209 ct WNT-induced FZD conformational changes in living cells in order to assess WNT action via FZDs at t

210 In this context, the number of living cells in suspensions was directly determined util

211 y of two cell signaling pathways in the same living cells in tissues.

212 oncentration remains largely untested within living cells, in which the richly multicomponent nature

213 t are actively and selectively secreted from living cells independently of the classical endoplasmic

214 ecordings of chemically fixed and untreated, living cells indicate that the characteristic lattice di

215 Analysis of protein-protein interactions in living cells indicated that the ZNF277-uS5 complex is fo

216 introduce a cell-in-the-loop approach where living cells interact through in silico signaling, estab

217 The cultivation of human living cells into scaffolding matrices has progressively

218 tive means to deliver functional proteins to living cells is a central problem in biotechnology and m

219 cognate chemical communication channels with living cells is an important challenge for synthetic bio

220 sensitive imaging of telomerase RNA (TR) in living cells is crucial for improved guidance in cancer

221 e nanoscale, our work reveals that the ER in living cells is not limited to uniform sheets and tubule

222 but the acquisition of such information from living cells is still demanding.

223 While visualization of viral proteins in living cells is well developed, imaging of viral RNAs ha

224 ucleotide variants (SNVs) into DNA or RNA in living cells - is one of the most recent advances in the

225 tool for characterizing protein complexes in living cells, it was recently demonstrated that conventi

226 fully comprehensive computational model of a living cell may be drawing closer to reality, but our an

227 anding the unique mechanism of Api action in living cells may facilitate the development of new medic

228 Neurons like other living cells may have ultraweak photon emission (UPE) du

229 ocesses, the nanomechanical responses of the living cell membrane have been elusive due to complexiti

230 ed to investigate nanoscale mechanics of the living cell membrane.

231 in payload delivery to between 75 and 97% of living cells of three species: B. dendrobatidis, B. sala

232 Living cells often identify their correct partner or tar

233 detailed tracking of transient metabolism in living cells on the subminute time scale has become amen

234 ovalently link virus-interacting proteins in living cells on UV exposure at different time points, an

235 tion of natural materials, or that integrate living cells or bioactive moieties, can respond to a ran

236 em with maleimide fluorophores, we generated living cells or purified proteins that bind but do not t

237 particles, e.g., on biological samples like living cells, or to measure mechanical properties like t

238 delivery and performing complex tasks within living cells/organisms.

239 osin-based production of force and motion in living cells, particularly in muscle, and for the interp

240 Fusion of identifiable components of the living cell post cyst formation is unknown in modern inv

241 Living cells proliferate by completing and coordinating

242 In a living cell, protein function is regulated in several wa

243 from three kinases simultaneously in single living cells, providing evidence for a role of Src famil

244 nvasive monitoring of molecular processes in living cells, providing insights on the mechanisms under

245 Nonetheless, in living cells PSEN1/gamma-secretase transiently visits AD

246 interactions and holistic changes within the living cell remain invisible.

247 organization and transcriptional activity in living cells remains unclear.

248 rks, its mechanical role in the cytoplasm of living cells remains unknown.

249 to engage different cytoplasmic proteins in living cells - remains untested due to the complexity of

250 he ability to engineer feedback control into living cells represents an important milestone in achiev

251 Synthetic assembly within living cells represents an innovative way to explore pur

252 pendent chemical trapping of unfolded G4s in living cells reveal that G4s fluctuate between folded an

253 sualizing siRNA targeting of single mRNAs in living cells reveals that passing ribosomes temporarily

254 on of Al(3+) is tested in milk as well as in living cells (Saccharomyces cerevisiae and Debaryomyces

255 Living cells segregate molecules and reactions in variou

256 The SHL probe loaded in living cells shows signature chemical properties of ioni

257 In living cells, sptPALM revealed PSEN1/gamma-secretase mai

258 t that forces impact biological responses of living cells such as gene transcription via previously u

259 es to lipids and E-cadherin molecules at the living cell surface.

260 is sufficient to detect light emission from living cells that is directly proportional to the number

261 t-mediated transport system is selective for living cells that produce biosurfactants.

262 ion imaging of ORAI1, STIM1, and septin 4 in living cells that septins facilitate Ca(2+) signalling i

263 ot suitable for samples, such as proteins or living cells, that are often available in limited volume

264 However, because this domain is essential in living cells, the functional requirement of the full CTD

265 l that can specifically mark tubulin PTMs in living cells, thus severely limiting our understanding o

266 noninvasive imaging of small biomolecules in living cells, tissues, and organisms.

267 An adequate response of a living cell to the ever-changing environment requires in

268 s on imaging DNA and single RNA molecules in living cells to define eukaryotic functional organizatio

269 agenesis and electrophysiological methods in living cells to demonstrate that NaRs can be converted i

270 loit the information-processing abilities of living cells to diagnose disease and then treat it in a

271 d proteins (IDPs) is a remarkable feature of living cells to dynamically control intracellular partit

272 design can be expanded to different types of living cells to fulfill diverse biological tasks.

273 Here, we engineer living cells to tag glycans with editable chemical funct

274 It has not been possible, in living cells, to precisely characterize the respective c

275 itative characterization of CTCF clusters in living cells, uncovers the opposing effects of cohesin a

276 or the spontaneous formation of OR dimers in living cells under physiological conditions is missing.

277 Two examples of kinetic studies of living cells under various doses of drugs are presented.

278 ent intracellular viscoelastic properties of living cells using multifrequency excitation and in situ

279 Reconstructing the functions of living cells using nonnatural components is one of the g

280 ified the possible MEHP-targeted proteins in living cells using the cellular thermal shift assay (CET

281 ments and can be attached to proteins within living cells using unnatural amino acid (UAA) mutagenesi

282 Actin in living cells was labeled with 580CP and 610CP probes, an

283 To investigate protein state transitions in living cells, we attached Cry2 to Tau and studied the co

284 Using super-resolution imaging of living cells, we find that PIP2 is tightly colocalized w

285 RPB1 (the largest subunit of RNA Pol II) in living cells, we identified Pol II as a direct gene-spec

286 ntation of fluorescently labeled proteins in living cells, we mapped the local alignments and the tim

287 By measuring DNA mobility in living cells, we show that condensed chromatin also exhi

288 ne of the most fundamental compartments of a living cell, where key processes such as selective trans

289 Polyamines are abundant in living cells, where they have physiologically important

290 recovery after photobleaching experiments in living cells, which expressed different versions of the

291 have been studied by tracking viruses within living cells, which limits the precision with which fusi

292 The wings of Lepidoptera contain a matrix of living cells whose function requires appropriate tempera

293 rated FGF2 oligomers at the inner leaflet of living cells with a FGF2 dimer being the most prominent

294 e kinetics of FGF2 membrane translocation in living cells with an average duration of ~200 ms.

295 hods, selective visualization of glycogen in living cells with high spatial resolution has proven to

296 nano-electronic interface to the interior of living cells with integrated fluorescence readout of met

297 small molecules, enzymes, nanoparticles and living cells within hours.

298 er, approaches that allow us to probe G4s in living cells without perturbing their folding dynamics a

299 s representative of the population of G4s in living cells, without globally perturbing G4 formation a

300 Cell) aim to build an entity that mimics how living cells work.