ライフサイエンスコーパス: long-term culture

1 differentiated state for more than 30 days (long-term culture).

2 the absence of a specific gene when grown in long term culture.

3 llagen matrix persisted and increased during long-term culture.

4 e interior regions of the organoid, enabling long-term culture.

5 as generally been problematic, especially in long-term culture.

6 r also exhibited homogeneous inducibility in long-term culture.

7 tinued to express CD34 throughout the 5-week long-term culture.

8 erminal differentiation in vitro, even after long-term culture.

9 rvived longer than wild-type spleen cells in long-term culture.

10 fferentiation of cord blood CD34(+) cells in long-term culture.

11 and assess adenoviral-associated toxicity in long-term culture.

12 tained from a single mouse and maintained in long-term culture.

13 CD34(+) and CD34(+)38(-) clonogenic cells in long-term culture.

14 was statistically associated with successful long-term culture.

15 ting clones, 17A and 17B, were maintained in long-term culture.

16 sc composition, structure, and function with long-term culture.

17 r/tibia HSCs, including clonogenic assay and long-term culture.

18 unable control over cell-contact time during long-term culture.

19 mortalizes human CD34(+) cord blood cells in long-term culture.

20 ary cell population that can be amplified in long-term culture.

21 and its deficiency promoted HSC expansion in long-term culture.

22 ibution is not altered by immortalization or long-term culture.

23 oblast differentiation and mineralization in long-term culture.

24 aSCs (cMaSCs) lose their growth potential in long term cultures.

25 e diffusion limit to prevent cell death over long-term cultures.

26 ormed, cultured human corneal fibroblasts in long-term cultures.

27 on and increased survival in both short- and long-term cultures.

28 e survival of HIV-specific CD8(+) T cells in long-term cultures.

29 are fully functional and equally expanded in long-term cultures.

30 ansfer using embryonic fibroblast cells from long-term cultures.

31 Bovine CECs were grown and aged as long-term cultures.

32 regulatory T cells that can be maintained in long-term cultures.

33 the glucocorticoid induction of apoptosis in long-term cultures.

34 virus serotype 16 was necessary to establish long-term cultures.

35 cursors for colony-forming cells detected in long-term cultures.

36 e emergence of SCH772984-resistant clones in long-term cultures.

37 ys, which require large numbers of cells and long-term cultures.

38 led CD19+ leukemic cells more effectively in long-term cultures.

39 o be examined in hPSC cardiomyocytes include long-term culturing, 3-dimensional tissue engineering, m

40 However, long-term culture (4-14 d) of DC in SMG reduced the expr

41 fferentiated into cardiomyocytes, even after long-term culture (50 passages or approximately 260 popu

42 Our method allows long-term culture (~50 days, 10 passages tested, accumul

43 Long-term cultures (72 h postinfection) of infected phag

44 ector was seen for over 15 weeks of extended long-term culture (9.2 +/- 5.2%, n = 7).

45 These data show that under long-term culture adult S. neumayeri appear to acclimate

46 topoietic progenitors detected in short- and long-term culture analyses.

47 ched during reprogramming to the iPSC stage, long-term culture and differentiation into target cells.

48 utant iPSC-MNs display decreased survival in long-term culture and exhibit hnRNP A2/B1 localization t

49 o establish a cell line that is suitable for long-term culture and large-scale in vitro experimentati

50 se, heterogeneity, and intransigence to both long-term culture and molecular or genetic modification

51 ented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small popu

52 itive hematopoietic potential as measured by long-term culture and phenotypic analysis.

53 functional islet tissue can be maintained in long-term culture and successfully transplanted.

54 nephron stem cell niche and hold promise for long-term culture and utilization of these progenitors i

55 e previously defined using NK cell clones in long-term culture and with the frequencies of cells expr

56 cells derived from marrow and cord blood in long-term cultures and long-term culture-initiating cell

57 te and maintain leukemic cell growth in both long-term cultures and nonobese diabetic/severe combined

58 when the primary lymphomas were subjected to long-term culture, and completely missed in the standard

59 ndifferentiated, pluripotent ES cells during long-term culture, and maintain their potential to diffe

60 ion and maturation), cellular composition in long-term culture, and the transcriptome of the two cult

61 e is designed to maintain cell viability for long-term culture as well as to introduce exogenous reag

62 ome, produced very low levels of virus after long-term culture, as previously reported in other astro

63 NOD/SCID) mice, and in primary and secondary long-term culture assays.

64 phases do not separate from each other) over long-term culture (at least 3 weeks) and support spatial

65 In these long-term cultures, bone marrow microvascular endothelia

66 creases in parallel with the loss of CD28 in long-term cultures, but this effect is blunted in the pr

67 ted beta-catenin expands the pool of HSCs in long-term cultures by both phenotype and function.

68 analysis at the individual cell level during long-term culture, by FISH techniques, reveals chromosom

69 However, reprogramming and long-term culture can also induce abnormalities in these

70 whether adult self-renewing spermatogonia in long-term culture can generate such stem cells as well.

71 d for in vitro stem-cell-like multipotential long-term culture capability.

72 Long-term cultured cells of this kind retained some pote

73 In addition to experiments on long-term cultured cells, we also found that wild-type p

74 In long-term cultures, cells maintain their morphology, for

75 were seen in cultured chondrocytes, a stable long-term culture chondrosarcoma cell line, as well as C

76 s) and more primitive stem/progenitor cells (long-term culture colony-forming cells) could be shown a

77 32D/EGFRvIII P5 cells subjected to long-term culture conditions in the absence of IL-3 reve

78 lows for cross-species co-culture as well as long-term culturing conditions.

79 uman hepatocyte spheroids after 3-4 weeks of long-term culture confirmed the presence of the liver-sp

80 onsmoker and smoker control subjects, during long-term culture, COPD fibroblasts displayed increased

81 ls was sustainable yet reversible even after long-term culture, demonstrating the impact of mechanica

82 Long-term culture did not affect the chondrogenic potent

83 CLiPs in long-term culture did not lose their proliferative capac

84 Long-term cultures displayed a myelomonocytic morphology

85 o test somatic CAG-repeat alterations during long-term culture, DNA was extracted from transgenic tis

86 )KDR(+) cells were grown in 3-month extended long-term culture (ELTC) through 3 serial culture rounds

87 these iTreg displayed a stable phenotype in long-term cultures, even in the presence of proinflammat

88 ary mouse hepatocytes that also show GJIC in long-term culture for 30 days.

89 cells capable of sustaining hematopoiesis in long-term cultures for 5 weeks.

90 ststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic

91 Long-term cultures (> 50 population doublings [PDs]) wer

92 cytokines recently shown to maintain HSCs in long-term culture, had a more-than-additive effect in el

93 ator to maintain homogeneous inducibility in long-term culture has not been examined.

94 In the first stage (human B-progenitor long-term culture [HB-LTC]), CD34(+) hematopoietic proge

95 ells using retroviral vectors from short- or long-term cultured human and mouse amniocytes using four

96 Interestingly, long-term-cultured human epithelial cancer cells in clon

97 is emphasized by mitotic cells and cells in long-term culture, identifies a hitherto underappreciate

98 uccessful in maintaining trabecular cells in long-term culture if low perfusion rates occur.

99 h were investigated in airway epithelia in a long term culture in the absence of luminal infection, w

100 ability effect of the agonist was blocked by long-term culture in 25 mM glucose.

101 bited synaptic transmission between SCGNs in long-term culture in a time-dependent manner, significan

102 y calcium-carbonate-dependent organism after long-term culture in predicted end-century acidification

103 Many minichromosomes were lost after long-term culture in the absence of telomerase, which ma

104 can be stably maintained during continuous, long-term culture in the presence of drug selection.

105 However, resistant clones occur upon long-term culture in the presence of inhibitors.

106 ered tissues, allowing for ease of handling, long-term culture in vitro and anchoring of the central

107 ic progenitor cells remain pluripotent after long-term culture in vitro and that E2A proteins play a

108 they may undergo progressive adaptation upon long-term culture in vitro.

109 ype 1 (HIV-1) is frequently attenuated after long-term culture in vitro.

110 21 patients with MDS was then propagated in long-term cultures in the presence or absence of TRAIL.

111 6-infected primary cell lines established as long-term cultures in vitro was also evaluated at RNA an

112 However, during long-term culturing in BrainPhys, non-neuronal cells app

113 Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteo

114 ells to Pseudomonas aeruginosa obtained from long-term cultures inhibits Duox1-dependent hydrogen per

115 We have recently reported that long-term cultures initiated with CD34+CD38- cells from

116 effect on CFU-GM and BFU-E formulation or on long term culture initiating cells.

117 e LTC-IC period (35 to 60 days) and extended long-term culture initiating cell (ELTC-IC) period ( > 6

118 following chemotherapy and a higher leukemic long-term culture initiating cell potential, targeting m

119 We have demonstrated that long-term culture initiating cells (LTC-IC) are maintain

120 in -/34(+)/DRdim cells could generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as 1

121 We have recently shown that more than 90% of long-term culture initiating cells (LTC-IC) mobilized in

122 -forming unit-granulocyte-macrophage and for long-term culture initiating cells as compared with bone

123 lineage(-) and Sca(+) lineage(-) cells, and long-term culture initiating cells.

124 ells from patients with AML were enriched in long-term culture, initiating cells and repopulating cel

125 ormation from purified CD34+ marrow cells in long term culture-initiating cell assays.

126 e IGF-II-dependent enhancement of CFU-GM and long term culture-initiating cell numbers.

127 ophages (CFU-GM), and primitive progenitors, long term culture-initiating cells (LTC-IC) derived from

128 Long-term culture-initiating assays with CD34(+)/CD38(-)

129 not yet routine, in vitro assays such as the long-term culture-initiating cell (LTC-IC) assay have be

130 that IFN-gamma is a potent inhibitor in the long-term culture-initiating cell (LTC-IC) assay, the be

131 row and cord blood in long-term cultures and long-term culture-initiating cell (LTC-IC) assays.

132 ration on days 3, 5, and 7, then assayed for long-term culture-initiating cell (LTC-IC) function on d

133 Long-term culture-initiating cell (LTC-IC) maintenance w

134 omposed mostly of progenitors and cells with long-term culture-initiating cell (LTC-IC) potential.

135 with acquired aplastic anemia (AA) using the long-term culture-initiating cell assay (LTC-IC), in par

136 mitive leukemic cells capable of growth in a long-term culture-initiating cell assay and expansion on

137 on hematopoietic progenitors and increase in long-term culture-initiating cell assay cells (primitive

138 The inclusion of BCCs in stromal support of long-term culture-initiating cell assay frequencies show

139 Long-term culture-initiating cell assay, with or without

140 Long-term culture-initiating cell assays demonstrated a

141 Long-term culture-initiating cell assays on murine strom

142 Of relevance is the novel finding by long-term culture-initiating cell assays that showed an

143 The clone detected in the long-term culture-initiating cell compartment that recon

144 ut not CD34(+)ACE(-) cells, are endowed with long-term culture-initiating cell potential and sustain

145 y of FL and SCF to maintain the viability of long-term culture-initiating cells (25 and 32%, respecti

146 a 5 to 7 times higher frequency of leukemic long-term culture-initiating cells (L-LTC-IC) compared w

147 Long-term culture-initiating cells (LTC-IC) are arguably

148 Long-term culture-initiating cells (LTC-IC) are hematopo

149 We now show that long-term culture-initiating cells (LTC-IC) are maintain

150 tly demonstrated that 50% of primitive human long-term culture-initiating cells (LTC-IC) are maintain

151 phenotype of primitive progenitors, such as long-term culture-initiating cells (LTC-IC) in mobilized

152 elphia chromosome (Ph)- and BCR/ABL-negative long-term culture-initiating cells (LTC-IC) in selected

153 ain-specific differences in the frequency of long-term culture-initiating cells (LTC-IC) in the bone

154 progenitors as well the ability to maintain long-term culture-initiating cells (LTC-IC) in vitro.

155 Efficient gene transfer to progenitors and long-term culture-initiating cells (LTC-IC) was obtained

156 y 12 colony-forming units-spleen (CFU-S), or long-term culture-initiating cells (LTC-IC), suggesting

157 orming unit (CFU-S) assay and in vitro using long-term culture-initiating cells (LTC-ICs) and methylc

158 f cobblestone area-forming cells (CAFCs) and long-term culture-initiating cells (LTC-ICs) in a transw

159 ced survival, serial replating capacity, and long-term culture-initiating cells (LTC-ICs) in LSCs fro

160 was not significantly altered, the number of long-term culture-initiating cells (LTC-ICs) was 1.5-fol

161 Furthermore, the number of long-term culture-initiating cells (LTC-ICs) within an a

162 that rhesus SP cells are highly enriched for long-term culture-initiating cells (LTC-ICs), an indicat

163 s (primary colony-forming cells [CFCs]), and long-term culture-initiating cells (LTC-ICs; a stem cell

164 Quantitative analysis of long-term culture-initiating cells (LTCIC) failed to dem

165 m immunophenotypic data, the effect of FL on long-term culture-initiating cells (LTCIC) was determine

166 etermine the effect of ANK on more primitive long-term culture-initiating cells (LTCIC), the IL-2-sup

167 R/ABL-positive primitive progenitors (6-week long-term culture-initiating cells [LTCICs]) as well as

168 Ph-negative long-term culture-initiating cells are detectable in man

169 ntly suppressed CML colony-forming cells and long-term culture-initiating cells but did not significa

170 hB4 depleted primitive cells, as measured by long-term culture-initiating cells or CD34(+)CD38(-) cel

171 er numbers of human colony-forming units and long-term culture-initiating cells per engrafted human C

172 Analysis of bcr/abl expression in long-term culture-initiating cells suggested that purgin

173 In contrast, even the frequency of long-term culture-initiating cells within the CD34(+) DC

174 le to generate CFU beyond 60 days ("extended long-term culture-initiating cells" or ELTC-IC) are func

175 e-macrophage colony-forming units) and late (long-term culture-initiating cells) hematopoietic progen

176 colony-forming units in methylcellulose, and long-term culture-initiating cells) occurred several day

177 l blood was not associated with detection of long-term culture-initiating cells, consistent with the

178 dition, Tpo promoted viability of CD34+CD38- long-term culture-initiating cells, further supporting t

179 med by a dramatic increase in the numbers of long-term culture-initiating cells, the most primitive h

180 ransduction of both colony-forming units and long-term culture-initiating cells, with transduction ef

181 the notion that SRCs are more primitive than long-term culture-initiating cells.

182 Transcriptomic landscapes of long-term cultured iPSC-CMs allowed mapping of distinct

183 ia species, Plasmodium falciparum, for which long term culture is possible, and Plasmodium vivax, for

184 possible, and Plasmodium vivax, for which no long-term culture is feasible.

185 Long-term culture is known to achieve partial maturation

186 A major obstacle to long-term culture is that in vitro mitogens quickly driv

187 layers, the use of classic stroma-dependent long-term cultures is not possible.

188 We evaluated the use of long-term cultured islet cells for the treatment of diab

189 presynaptic VAMP2 and postsynaptic PSD95 in long-term cultured live primary neurons in 96 well micro

190 CR and CNR cells were cultured in secondary long-term cultures (LTCs) and assayed weekly for transdu

191 Taken together, our results demonstrate that long-term cultures maintained on multi-electrode arrays

192 h stromal ligands, hydrocortisone-containing long-term culture medium, IL-2, IL-7, and stem cell fact

193 ns poorly known, mainly due to the lack of a long-term culture method for this parasite.

194 tissues are dominated by midgut derivatives, long-term-cultured microdissected hepato-biliary-pancrea

195 bstrate-integrated microelectrode arrays and long-term cultured neuronal networks.

196 Long term culture of MCF-7 cells in estrogen (E2)-deplet

197 As expected, telomerase activity declined in long term culture of stable transfectants.

198 vage of fibrocystin occurs constitutively in long term cultures of polarized inner medullary collecti

199 Short term and long term cultures of transfected HEK293 cells suggest t

200 Here, we show that long term culturing of cells in the presence of the LRP-

201 Interestingly, long-term culture of a virus lacking the BHA cytoplasmic

202 We established conditions for long-term culture of adult mouse cardiomyocytes that are

203 e plated under Whitlock-Witte conditions for long-term culture of B lineage cells.

204 Long-term culture of B-CLL clones would permit the colle

205 stem cell properties can be derived from the long-term culture of diverse tissues, it is not clear wh

206 Long-term culture of EBV+ lymphoblastoid cells in IFN-al

207 Long-term culture of EIAV-transduced human cells showed

208 Finally, via long-term culture of gastric tumour epithelium, we revea

209 xpansion within the mutant HTT allele during long-term culture of HD cells.

210 To promote long-term culture of human islets in PIM-R medium (used

211 Long-term culture of infected Huh7.5 cells with increasi

212 This platform could support long-term culture of intestinal organoids, potentially r

213 olic output, and cellular homeostasis during long-term culture of iPSC-CMs.

214 velopmental progression, as recapitulated by long-term culture of iPSC-CMs.

215 Our results show that SFM allows for the long-term culture of islet tissue.

216 A new method was developed to permit the long-term culture of islets.

217 We show that CDF can support the long-term culture of laboratory strains and demonstrate

218 In the present study, a long-term culture of M. avium-M. intracellulare-infected

219 This approach allowed the long-term culture of mouse myeloid progenitors (HoxB8 pr

220 vaccine is the only option in the absence of long-term culture of P. vivax parasites.

221 We used short-term and long-term culture of pal-mutated viruses in permissive C

222 nize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.

223 Long-term culture of primary human cells modified with e

224 been limited by the lack of methods for the long-term culture of primary human intestinal epithelial

225 Long-term culture of primary murine small intestinal epi

226 We describe a method for long-term culture of primary small intestinal epithelial

227 g a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specime

228 the EBV P3HR-1 strain, we have reproduced in long-term culture of SVK epithelial cells an unusual pat

229 Long-term culture of transfectant clones in the absence

230 In long-term culture of transfected MDA-MB-231 cells, expre

231 However, it has been difficult to establish long-term cultures of adenoma cells, especially those of

232 F74 did not detect viral gene transcripts in long-term cultures of bone marrow stromal cells from 23

233 signals in the cortical regions, short- and long-term cultures of E14.5 telencephalic progenitors we

234 Long-term cultures of GPR125+ SPCs (GSPCs) also converte

235 developmental progression, we have generated long-term cultures of hematopoietic progenitors by enfor

236 show that modulation of TNF-alpha levels in long-term cultures of human CD8(+) T lymphocytes, by chr

237 t the establishment, from mouse ES cells, of long-term cultures of immature DCs that share many chara

238 Sequence analysis of viral DNA derived from long-term cultures of Jurkat cells revealed a specific m

239 s observed in conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells M

240 es containing conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells w

241 ecruitment to conditioned media derived from long-term cultures of mouse Lewis lung carcinoma cells.

242 virus (HCV) infection require examination of long-term cultures of normally differentiating primary h

243 Treatment with NGF causes long-term cultures of oligodendrocytes to die via a yet

244 Long-term cultures of SV40-infected human keratinocytes

245 Long-term cultures of T cells and FLS form heterotypic f

246 ve protein (PARP) fraction was purified from long-term cultures of Trypanosoma brucei procyclic forms

247 Long-term cultures of unstimulated mouse HSCs secreted I

248 We report the derivation of long-term cultures of untransformed DCs, uniformly expre

249 day 14, and expression of IL-10 continued in long-term cultures of up to 120 days.

250 A PIV5 PI cell line established by long-term culturing of acutely infected A549 cells showe

251 ia-BRCA pathway, became perturbed only after long-term culturing of infected cells.

252 ould make them useful laboratory models, but long-term culturing of tardigrades historically has been

253 lly isolated from the relevant T cells after long-term culture, often after repeated antigen stimulat

254 y acquire the ability to form colonies after long-term culture on bone marrow stroma, coincident with

255 ng cell (CAFC) subsets in stromal-associated long-term cultures on fresh and frozen PBPC.

256 Porcine hematopoiesis can be maintained in long-term cultures on primate stroma with pig cytokines.

257 a limited fashion without measurable loss of long-term culture or in vivo engrafting potential as mea

258 freshly isolated, short term activated, and long term cultured PBT.

259 theca cells from PCOS ovaries maintained in long term culture persistently secrete significantly gre

260 s (apoptotic indices) between the short- and long-term culture-positive animals were not different.

261 not suitable for studies with live cells in long-term culture prior to analysis.

262 t study, we showed that, although a panel of long-term cultured rat uveitogenic T cell lines specific

263 ted to a small number of lineages, and their long-term culturing remains problematic.

264 We conclude that long-term cultured rhesus macaque spleen-derived Valpha2

265 Progenitor cells from long-term cultures showed altered expression of genes de

266 lretinin and bromodeoxyuridine antibodies in long-term cultures showed that only a few mitotic utricu

267 IHK-beta-globin transgenes from silencing in long-term culture studies.

268 n a newly developed, stromal cell-dependent, long term culture system, the ability of selected thymic

269 Furthermore, development of a long-term culture system for rat SSCs has established a

270 We evaluated HCV-IFN interactions within a long-term culture system of Huh7 cell lines harboring di

271 Here we present a long-term culture system of the normal mouse urothelium

272 Long-term culture systems have played a pivotal role in

273 early lethality of the Tbx1-/- mice, we used long-term culture techniques that allow the unharmed gro

274 ansgene were observed in cells maintained in long-term culture that had been infected with the LA vec

275 In MS-5 feeder cell-based long-term cultures that supported the growth of primary

276 When maintained in long term-culture, the L2198S RNA evolved into a stable

277 al endothelial cells which remain diploid in long-term culture, the aneuploidy of tumor endothelial c

278 In long-term cultures, the granular layer appeared well pre

279 escribed, it has become apparent that during long-term culture these cells (collectively referred to

280 D34+ HLA-DR+ mobilized PB cells can initiate long-term cultures, they are relatively mature and canno

281 During long-term culture, this circuitry was found to initiate

282 activity in vivo and maintain the ability in long-term culture to give rise to multipotent adult sper

283 e the requirement for in vitro activation or long-term culture to introduce the transgene and obtain

284 d used marrow-derived preadipocyte lines and long-term cultures to explore potential roles in hematop

285 In long-term cultures, typical cellular morphology and pigm

286 This study examined the effects of long-term culture under altered conditions on the Antarc

287 ighly oligotrophic conditions which disabled long-term culturing under laboratory conditions.

288 tilage biopsies of patients and subjected to long-term culture undergo dedifferentiation and that the

289 Normal dog gallbladder epithelial cells in long-term culture were used as a model to study the morp

290 Four long-term cultures were established from 39 attempts.

291 city of T cells from fresh leukocytes and of long-term cultures were monitored by flow cytometry.

292 exhibit enhanced proliferative responses in long-term cultures when stimulated to divide with antibo

293 prediabetic adult non-obese diabetic mice in long-term cultures, where they were induced to produce f

294 is a functional hierarchy of progenitors in long-term culture which correlates with their level of q

295 al respiratory rate and enzyme activities by long-term culture with 2 mmol/L adenosine 5'-diphosphate

296 king the p53-inactivating domain to maintain long-term cultures with a p53-responsive phenotype.

297 ially induce migration in Th1 cells, and, in long-term cultures with IL-2, IL-16 facilitates the expa

298 3 dimensional growth in agarose gels and in long-term cultures within matrigel.

299 phase is also corrupted in cells adapted to long-term culture (WT or CIZ1-null), and also accompanie

300 T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes.