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1 lowing stimulation with an EBV-infected cell lysate.
2 uantification of unlabeled compounds in cell lysate.
3 0 phosphopeptides from 100 mug of MCF-7 cell lysate.
4 d perform western blotting on each resultant lysate.
5 ivated by a Mycobacterium tuberculosis (Mtb) lysate.
6 cy (RO) for a covalent binding drug in blood lysate.
7 rification of nucleic acids from the sampled lysate.
8 fectively used with purified protein or cell lysate.
9 d for the detection of ERalpha in MCF-7 cell lysate.
10 dvantageous to measure miRNA in a crude cell lysate.
11 de novo sequencing using whole cell E. coli lysate.
12 andard deviations of up to 70% in crude cell lysate.
13 ough on-chip electrical sensing of bacterial lysate.
14 en when dispersed in BSA or Escherichia coli lysate.
15 minant AEBSA target in diluted serum or cell lysate.
16 re protective compared to an uninfected cell lysate.
17 purity of >90% mitochondrial proteins in the lysate.
18 ered saline (PBS) and spiked in E. coli cell lysate.
19 of the total protein from breast cancer cell lysates.
20 demonstrated in food samples using crude DNA lysates.
21 rified esterases in bacterial and human cell lysates.
22 ment showed accumulation of LC3B II in heart lysates.
23 ing either protein standards or complex cell lysates.
24 ll as profiling glycosylase activity in cell lysates.
25 either FLAG or DYKD epitopes from crude cell lysates.
26 munoprecipitated from Schwann cell and nerve lysates.
27 es endogenous KCTD proteins from mouse brain lysates.
28 ins that bind to rRNA G-quadruplexes in cell lysates.
29 mounts of ADP-ribosylated peptides from cell lysates.
30 tivity of caspase-7 in Escherichia coli cell lysates.
31 ted and thioesterified forms of Ub from cell lysates.
32 m and function were also evaluated in tissue lysates.
33 vivo with parasite schizont and erythrocyte lysates.
34 y broad PcG domains in wildtype whole embryo lysates.
35 kines were measured in plasma and neutrophil lysates.
36 avage of PrP(C) is observed in mouse retinal lysates.
37 detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates.
38 -purified with PfMyoA isolated from parasite lysates.
39 tryptase were measured in plasma and biopsy lysates.
40 ies recognized native Siglec-E within spleen lysates.
41 exposed to P. falciprum infected erythrocyte lysates.
42 to profile molecular activities within cell lysates.
43 ly active Fic(E247G) mutant in Drosophila S2 lysates.
44 y of ITPA in bacterial, yeast and human cell lysates.
45 roteins, protein complexes or clarified cell lysates.
46 and CXCL2 were reduced in Il1a(-/-) oviduct lysates.
47 e the endogenous protein from mammalian cell lysates.
48 ltiple Ral-effector interactions in cellular lysates.
49 )-dependent desuccinylation activity in cell lysates.
50 culture media, but increased it in the cell lysates.
51 pattern of respective OH-metabolites in fly lysates.
52 g yeast, wheat germ, and rabbit reticulocyte lysates.
53 eracted with the P. falciparum-infected cell lysates.
54 coverage when applied to S. pombe whole cell lysates.
55 on of NTH1 activity in vitro and in cellular lysates.
56 ',4,6'-trichlorobiphenyl (3'-OHCB 28) in fly lysates.
57 cted immunoreactive IL-27 (953 +/- 504 pg/mg lysate), a mediator not previously associated with human
58 injected systemically, coupled with necrotic lysates, a higher number of large lesions was detected i
60 tumor growth relative to simple mixtures of lysate and CpG-1826 (Lys-Mix) in both Py230 and Py8119 o
61 cantly, and simultaneously upregulated whole lysate and membrane portion NKCC1 protein expression.
63 ion of proteins from minimal amounts of cell lysate and opens more opportunities for the isolation of
64 itro translation assay containing human cell lysate and purified target mRNA fused to a reporter was
65 ning a ribosome-depleted rabbit reticulocyte lysate and ribosomes prepared from HeLa or Huh-7.5 cells
66 oncentration was measured for mammalian cell lysate and serum, which led to estimates of low-mM ribos
67 trazole probe was also assessed in HeLa cell lysate and showed robust labeling only upon photoactivat
68 his iBody for the isolation of FAP from cell lysates and blood serum as well as for its detection by
69 as also measured by ELISA in bronchial/nasal lysates and by immunohistochemistry in bronchial tissue
71 c comparison between metaphase-arrested cell lysates and chromosome-sorted samples reveals a cohort o
72 e mouse Htt transcripts directly from tissue lysates and demonstrate that this can replace the more w
73 AX-haptenated proteins were detected in cell lysates and extracellularly, either as soluble proteins
74 e P300/CBP-associated factor (PCAF) in liver lysates and inhibited its activity in vitro This study t
76 V system, we purified AAVs from HEK293T cell lysates and medium by polyethylene glycol precipitation
77 also increased phospho-VEGFR2 in whole cell lysates and membrane fractions compared with control siR
78 ve assessment of all Htt transcripts in cell lysates and mouse tissues without the need to first extr
82 aneous detection of two miRNAs in crude cell lysates and proved that the method was robust when used
83 agged cardiac myocyte ribosomes from cardiac lysates and subjecting the ribosome-protected mRNA fragm
84 Gag peptides were detectable in both cell lysates and supernatants in CD4+ T cells infected in vit
85 yn I serine 9, site 1, in the frontal cortex lysates and synaptosome preparations of male rhesus maca
86 uccessfully pull-down peptide-MHCs from cell lysates and the aAPCs generated using this technique wer
88 was verified with model samples of bacterial lysates and with four real-matrix samples of knee joint
90 idoyl fluoride electrophile, with human cell lysate, and the protein conjugates formed were identifie
92 phosphopeptides in clinical specimens, cell lysates, and mouse liver tissue samples, demonstrating i
94 on of neutrophil elastase activity in tissue lysates, and we have applied it to demonstrate that this
95 ssolved organic carbon from Emiliana huxleyi lysates, and were conducted using surface-seawater colle
96 Previous investigations using Leishmania lysate antigen demonstrated that the immunoglobulin (Ig)
97 d immunosorbent assays (ELISAs) with rK39 or lysate antigens and novel IgG1 rK39 rapid diagnostic tes
98 e sought to evaluate the effect of bacterial lysate applied orally from the second until seventh mont
102 Together, these results show that the way lysates are processed and packaged has a profound impact
103 e assay-based kinome analysis (in which cell lysates are used to phosphorylate specific kinase substr
106 against the gold standard Limulus Amoebocyte lysate assay in real bacteria culture containing natural
107 tally show that Ca2+ application to cellular lysates at physiological concentrations inhibits PP2C ac
108 -interactive proteins from the S. coelicolor lysate based on the tandem affinity purification (TAP).
111 n HEK293T cells, we detected WT MESD in cell lysate but not in conditioned medium, whereas the conver
112 DH enzyme activity was detected in GV oocyte lysate, but CHDH became highly active during oocyte meio
113 o ability to suppress dicer activity in cell lysates, but higher expression of B2, following heat sho
114 o interrogate itaconate's reactivity in cell lysates, but methods for analyzing targets of itaconatio
115 on accurate miRNA quantitation in crude cell lysate by a CE-based hybridization assay termed direct q
118 values for unpurified proteins in crude cell lysates can be obtained without prior knowledge of the c
119 emonstrated that human CBD and PSP tauopathy lysates (CBD-tau and PSP-tau) contain distinct tau strai
120 high quantity of mRNA from crude yeast cell lysate compared to a phenol/chloroform extraction method
121 oscopy and elemental Zn in bio-chelated cell lysate complex was confirmed by SEM and Energy Dispersiv
122 atly increased in Nf123aIN/23aIN mouse brain lysates, confirming that exon 23a inclusion inhibits Nf1
123 allergic parent received orally a bacterial lysate consisting of heat-killed Gram-negative Escherich
125 We previously demonstrated that crude cell lysates could be used for some limited downstream DNA ap
126 dard data set and a public large-scale yeast lysate data set show that iTop-Q achieves highly accurat
127 mparison of adult bone marrow to fetal liver lysates demonstrated developmental silencing in IGF2BP1.
128 alyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the utility of
129 al method for measuring enzyme activities in lysates derived from individual cells and will contribut
131 in-labeled viral dsRNA or poly(I.C) and cell lysate-derived or in vitro translated G3BP1 indicated th
132 of mdDiLeu-labeled Saccharomyces cerevisiae lysate digest on an Orbitrap Fusion Lumos mass spectrome
136 ines (MAV) prepared from S. pneumoniae TIGR4 lysates enriched for surface proteins by a chromatograph
137 In addition, our sensor could detect SP lysate even when dispersed in BSA or Escherichia coli ly
138 es of SGPs could be detected from total cell lysates, even at a signal-to-noise ratio of as low as 3:
140 ein levels using 0.5 ug/sample total protein lysate extracted from 2 x 5 um FFPE slices absolutely an
141 with dried blood spot punches or fibroblast lysate followed by quantification of enzymatic products
144 Following cell lysis and sampling crude cell lysate for analysis, the substrate and the product are s
145 -scale proteomics analysis of cell or tissue lysate for enhanced proteome coverage, particularly for
146 mensionality of datasets is shown using cell lysates for all possible combinations of up to 9 fingerp
151 ferently to syndecan-4 in the left ventricle lysate from aortic-banded heart failure (ABHF) rats comp
152 f both CXCR4 and CXCR7 compared with protein lysates from a nontumorigenic prostate epithelial cell l
155 sates containing baculovirus-expressed B2 to lysates from dipteran (S2, Aag2) or lepidopteran (Sf9) c
160 e-affinity pulldown assays and proteomics of lysates from primary cortical neurons, we identified tha
161 ly, MLC2 phosphorylation was reduced in lung lysates from TIMAP-deficient mice and upon silencing of
162 ll types is challenging due to the fact that lysates from tissues always represent a mixture of cells
164 sis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention information fo
167 d the presence of chlorotyrosine in the cell lysates, hypochlorous acid is hypothesized to be the che
169 detected in detergent-treated infected cell lysates.IMPORTANCE The entry of enveloped viruses is ach
171 exhibits good sensing performance toward SP lysate in a clinically relevant linear range from 5 to 1
173 ng cation exchange-fractionated HEK293T cell lysate in XL-MS experiments using disuccinimidyl sulfoxi
175 compound 73 enriched NS4B protein from cell lysates in pull-down studies, and the findings together
176 tide-MHCs can directly be captured from cell lysates, including cancer cells using affinity beads, an
177 iple Dhb-modified proteins in mammalian cell lysates, including histone H3, a previously unknown targ
178 lli-Q(TM) water and the infiltration of cell lysate into the porous matrix was monitored by measuring
182 toward the potential of oxidized tumor cell lysate-loaded SNAs as a potent class of immunotherapeuti
183 stant oligomers change in size over time and lysates made from de novo induced cultures are able to c
184 strated that, compared to standard bacterial lysates, MAV was enriched with Hsps and contained severa
188 popolysaccharides (LPS) with and without PRF lysates obtained by repeated freeze-thawing or the secre
189 ab27b protein levels were increased in brain lysates obtained from postmortem tissues of individuals
190 phA7 could be precipitated from the cellular lysate of BJAB cells using recombinant gH/gL, and knocko
193 Bruton's tyrosine kinase (BTK) in the blood lysate of monkeys after dosing with branebrutinib (BMS-9
194 mpanying HNSCC biomarkers from a single cell lysate of oral cancer cell cultures was demonstrated.
196 abolome from the (12)C-/(13)C-dansyl labeled lysates of 100, 1000, and 10000 cells, respectively.
197 was then used to bind candidate ligands from lysates of activated monocytes and analyzed by mass spec
198 ining the inositol phosphate levels in brain lysates of animals previously treated with melatonin.
199 ever, many techniques analyze fixed cells or lysates of bulk populations, in which information about
202 protein required further incubation with the lysates of human umbilical vein endothelial cells (HUVEC
204 The immunomodulatory character of pancreatic lysates of patients with cancer differs from those with
205 oll-like receptor signaling were enriched in lysates of podocytes treated with the TrkC ligand neurot
207 se in CEST-MRI contrast was obtained in cell lysates of rdLRP transfected cells and in in vivo LRP ex
209 ody-based serotyping assay that uses culture lysates of Streptococcus pneumoniae This study describes
210 HIV trans-activation response element RNA in lysates of T cell lines or primary human CD4(+) T cells.
212 mining the numbers of CFU recovered from the lysates of the infected monolayers, by monitoring the le
217 the form of a defined peptide antigen, cell lysates or antigens sourced from AML cells recruited in
218 d transformation results collected from cell lysates or fixed-cells conceal important dynamic informa
220 ts as a biochemical precursor to NAD(+) Cell lysates possess an ATP-dependent kinase activity that ef
221 significantly increased cAMP levels in cell lysates prepared from inner medullary collecting duct (I
223 ll types, nc886 bound to PKR in human T cell lysates, preventing PKR phosphorylation by polyinosinic:
224 MT 10-plex experiments with one microgram of lysate protein and 2000 sorted HeLa cells (roughly half
225 a linear range of 0.05-2.0 fmol/mug of total lysate protein and with coefficients of variation < 15%.
227 ectrometric analysis indicated that 628 cell-lysate proteins enriched in 44 cellular component cluste
229 nts with GBM undergoing treatment with tumor lysate-pulsed DC vaccination and PD-1 mAb blockade were
234 exes from as little as 4 micrograms of input lysate, representing a 50-100-fold downscaling compared
235 ucrose gradient fractionation of the nuclear lysate resolved several ribonucleoprotein (RNP) complexe
237 rotein interactions in E. coli and HeLa cell lysates, respectively, identifying 1,158 and 3,301 uniqu
239 A-MB-231 xenografts were exposed to necrotic lysates, resulted in an increase in both proliferation a
240 determined for miRNA samples in a crude cell lysate, RNA extract from the lysate, and a pure buffer.
243 LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Sin
245 alization of BfrB-iron in P. aeruginosa cell lysates separated on native PAGE gels and stained for ir
246 Immunoprecipitation of ARC from mouse islet lysates showed ARC binds JNK, suggesting interaction bet
249 eover, the addition of purified RelE to cell lysates shows promise as a method for generating ribosom
250 rganic compounds and 0.2 mum filtered diatom lysate significantly enhanced the growth of this bacteri
251 mplexity were compared: (1) data from a cell lysate study performed in our lab, (2) data from an inte
253 d human patients compared with lymphoma cell lysates, suggesting a concentration of these factors ove
255 ndent desuccinylation activity in crude cell lysate systems, as compared to the desuccinylation react
256 tion identified Hspb1 mRNA in wild-type lens lysate TDRD7-pulldowns, and single-molecule RNA imaging
257 tivities in dried blood spots and fibroblast lysates that allow newborn screening and diagnosis of al
258 ded cellular environment and eukaryotic cell lysates, that parameters optimized toward a pseudo first
259 protein could be obtained directly from cell lysates, thus leading to an improved method to recombina
260 for sensitive and selective analysis in cell lysates, tissues, and serums by mass spectrometry (MS).
264 an embryonic kidney cells and cardiac tissue lysates to enable the identification of 188 and 124 endo
267 ual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane micro
268 n of inflammatory markers in the lung tissue lysates using reverse transcription quantitative polymer
269 man and mouse GBM extracellular nanovesicles lysates using the Ras-binding domain of Raf also copreci
271 lysis (SCX-RPLC) of S. cerevisiae whole cell lysate was used to generate a retention dataset of appro
276 te middle-range sized peptides from cellular lysates, we explored the use of the proteases Asp-N and
277 ced conversion assays with RML-infected cell lysates, we observed a strong signal over all six passag
278 .250 nM for drug-bound BTK (DB-BTK) in blood lysate were successfully achieved by using this IC-LC-MS
280 increased levels of Cyc-D and p21 in spleen lysates were observed in animals administered control.
283 nd in complex environments of mammalian cell lysates, where relative amounts of free reactive oxygen
284 e spectral matches enriched from mouse brain lysates, which more than triples identifications from st
285 of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome sample
286 ct derivatization of AP sites in the nuclear lysate with O-(pyridin-3-yl-methyl)hydroxylamine, yieldi
287 lon cancer cell lines and flash-frozen tumor lysates with a linear range of 0.05-2.0 fmol/mug of tota
288 using proteome-wide reactions of HT-29 cell lysates with a model probe of threonine beta-lactone.
291 IIA heavy chain coimmunoprecipitated from EC lysates with endogenous TIMAP, and endogenous MLC2 coloc
292 ne cells when compared to simple mixtures of lysates with linear oligonucleotides both in vitro and i
293 tion can be performed in blood serum or cell lysates with minimal interference from biomolecules.
294 tation of rat liver or transfected HeLa cell lysates with rOATP1A1 antibody specifically co-immunopre
296 concentrations in the culture media and cell lysates without changing the expression of 5-HT synthesi
297 European Commission) using raw mitochondrial lysates without DNA extraction or polymerase chain react
298 ates, and also allows the direct use of cell lysates without the need for DNA purification or nuclear
299 ies and identified 1861 proteoforms in yeast lysate, yielding an approximately 40% increase over the
300 ntibodies against LcrV, Y. pestis whole-cell lysate (YPL), and F1 antigen and more balanced IgG1:IgG2