ライフサイエンスコーパス: lysogen

1 ith their host cell, forming a unit called a lysogen.

2 th L5 and efficiently forms plaques on an L5 lysogen.

3 particles following induction of a phage P22 lysogen.

4 ced Nun activity and Nun antigen in an HK022 lysogen.

5 mote positive evolutionary selection for the lysogen.

6 n wild-type cells with or without the SPbeta lysogen.

7 onstitutively transcribed phage genes in the lysogen.

8 -gene expression system of an induced lambda lysogen.

9 a mixture of total genomic fragments from a lysogen.

10 ated when fused to lacZ and tested as single lysogens.

11 c inability of rII mutants to grow on lambda lysogens.

12 ges because it encodes a repressor and forms lysogens.

13 ne accounts for the inability of D29 to form lysogens.

14 anscript in order to allow icd expression in lysogens.

15 rom growing on Escherichia coli lambda phage lysogens.

16 plays a crucial role in establishing stable lysogens.

17 ssed by the bacterial LexA protein in lambda lysogens.

18 omains to suppress biofilm formation in DMS3 lysogens.

19 tcdA, tcdB, tcdR, tcdE, and tcdC in PhiCD119 lysogens.

20 nt switch behavior upon induction of CTX Phi lysogens.

21 e, some cells lyse whereas others survive as lysogens.

22 Cr2010 and recently lost the ability to form lysogens.

23 e can account for the lower stability of Stx lysogens.

24 phage loci in B. anthracis and/or B. cereus lysogens.

25 se variants grew lytically and formed stable lysogens.

26 eakens binding by Lac repressor also induced lysogens.

27 the Siphoviridae family and produced stable lysogens.

28 ly into its host chromosome and forms stable lysogens.

29 to the Vibrio cholerae genome to form stable lysogens.

30 e of S. enterica serovar Typhimurium Gifsy-2 lysogens.

31 ther activated eib expression in the derived lysogens.

32 Specifically, the MAV1 lysogen 158L3-1 was more virulent than the nonlysogen st

33 Upon induction of a bacteriophage lambda lysogen, a site-specific recombination reaction excises

34 Through varphi24B conversion the lysogen also gains increased antimicrobial tolerance to

35 nt expression of a small non-coding RNA in a lysogen and in late lytic growth, although it is non-ess

36 ivity is not essential for integration; both lysogens and recombination intermediates are detected wh

37 Our results show that interactions between lysogens and sensitive strains are shaped by antagonisti

38 e of PhiCD119, was expressed in C. difficile lysogens and that its product, RepR, could downregulate

39 icates that Lac repressor was present in the lysogens and was necessary for stable lysogeny.

40 Nonetheless, 933W forms lysogens, and 933W prophage display a threshold response

41 This phage grows lytically, forms stable lysogens, and can switch from this regulatory state to l

42 uld grow lytically, could form highly stable lysogens, and carried out prophage induction.

43 to significantly reduce the stability of its lysogens, and may account for the hair-trigger nature of

44 s able to grow lytically, form stable single lysogens, and switch to lytic growth upon prophage induc

45 e sources in gene expression of phage lambda lysogen are quantified using models described by stochas

46 Lysogens are common at high bacterial densities, an obse

47 Because lysogens are generally found on the periphery of large c

48 assays for counting bacteriophages and their lysogens are labor-intensive and perturbative to the hos

49 o reduce local biofilm structural integrity, lysogens are predisposed to disperse into the passing li

50 Interestingly, Lula/phi80 lysogens are recD and sbcCD phenocopies, so GamL and Agt

51 that: (i) approximately 0.005% of the H-19B lysogens are spontaneously induced per generation during

52 Lysogens, bacteria with one or more viruses (prophages)

53 ase the expression of other phage genes in a lysogen because their transcripts should be terminated d

54 response to DNA damage and suggests how 933W lysogens behave as "hair triggers" with spontaneous indu

55 osmotic induction in lambdaphi(P3rpoH:lacZ ) lysogens, but had no effect on the activation of the dna

56 All 10 were capable of forming immune lysogens, but their original hosts were resistant to the

57 CI regulates its own synthesis in a lysogen by activating and repressing its promoter, P(RM)

58 ly arose via infection of an O139 CTX(ET)phi lysogen by CTX(calc)phi.

59 P(A) promoter, which is dually repressed in lysogens by the phage-encoded repressor RstR and the hos

60 ression system in which gpD deficient lambda lysogens can be co-complemented with both wild-type and

61 CTXPhi lysogens can be induced with DNA damage-inducing agents

62 Marvin is not temperate, and stable lysogens cannot be recovered from infections, although t

63 a culture spontaneously induce and when the lysogen carries two lambdoid prophages with different re

64 These lysogens carry an integrated L5 prophage inserted at a s

65 ble repressor fails to produce Stx, unlike a lysogen carrying a 933W derivative encoding a cleavable

66 A lysogen carrying a 933W derivative encoding a noncleavab

67 imit excisive site-specific recombination in lysogens carrying a single Mx8 prophage, which are less

68 nd studies utilizing Escherichia coli lambda lysogens carrying lacZ transcriptional fusions reveal th

69 which are less immune to superinfection than lysogens carrying multiple, tandem prophages.

70 nges involved in the lysis of induced lambda lysogens carrying prophages with either the lambda canon

71 e features of the new system are: (1) lambda lysogens carrying the fusion are made without regard for

72 gut metagenomic samples showed the ratios of lysogens (cells harboring prophages) to non-lysogens var

73 In a lysogen, CI represses the two lytic promoters, pR and pL

74 the accumulation of free phages in bacterial lysogen communities, thereby enhancing viral spread.

75 Furthermore, E. coli lambda phage lysogens complemented with B. burgdorferi recA produced

76 of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes

77 3 mutation confers thermoinducibility on N15 lysogens, consistent with CB being the primary repressor

78 bacteria, we used kanamycin-sensitive (KanS) lysogens containing a lambda kan- prophage.

79 Lysogens containing a single lambda kan- prophage per ba

80 ge Phi24(B) integrase expression in multiple lysogen cultures are demonstrated along with apparently

81 phi80 also did not change the phage titer in lysogen cultures, whereas the host dam mutation did incr

82 Studies with an E. coli lasB::lacZ lysogen demonstrated that RhlR multimerization was neces

83 gly, the intrinsic stability of lambdaprm240 lysogens depended markedly on the growth conditions; lys

84 g whether the HIV-1 Rev protein could direct lysogen development for bacteriophage derivatives that e

85 tion from the lambda origin was inhibited by lysogen-encoded cI repressor.

86 The derived lysogens express little or no Eib protein, in sharp cont

87 t protein nor super-repressor mutants induce lysogen formation for a P22 phage encoding an RNA hairpi

88 The wild-type coat protein directs efficient lysogen formation for P22 phages that carry several frag

89 The R17/MS2 coat protein efficiently directs lysogen formation for P22 R17 , a bacteriophage P22 deri

90 nding properties since they direct efficient lysogen formation for P22 R17 and P22 R17 [A(-10)U]; how

91 Phage lysogen formation occurs efficiently in recipient cells,

92 fe cycles after infection-host survival with lysogen formation, or host lysis and phage production.

93 HK022 prophages protect lysogens from superinfection by producing a sequence-spe

94 In CTX lysogens, gene expression originating from the rstA phag

95 depended markedly on the growth conditions; lysogens grown in minimal medium were nearly stable but

96 The SIVET lysogen has a defective H-19B prophage encoding the TnpR

97 In some cases rare lysogens have been formed in cells that belong to a muta

98 ugh lysogeny was rarely observed because the lysogens have increased death rates due to prophage indu

99 ause of a change in cI could not form stable lysogens; however, this defect could be suppressed by th

100 production of RstR(calc) renders a CTX(calc) lysogen immune to superinfection by CTX(calc)phi but sus

101 iscriminate between MC1061 and the varphi24B lysogen in standard culture, and when treated with 2 ant

102 (iv) Only a small fraction of the lysogens in a culture spontaneously induce and when the

103 Growth of H-19B lysogens in low iron concentrations or in conditions tha

104 Phage RedRock forms stable lysogens in Mycobacterium smegmatis in which the prophag

105 racterized mycobacteriophage L5 forms stable lysogens in Mycobacterium smegmatis.

106 racterized temperate phage that forms stable lysogens in Mycobacterium smegmatis.

107 eriophage L5 is a temperate phage that forms lysogens in Mycobacterium smegmatis.

108 We estimate that HK022 lysogens in stationary phase contain several hundred mol

109 Another variant could form stable lysogens in the presence of a ligand for Lac repressor b

110 yle but are apparently unable to form stable lysogens in their hosts.

111 phi in culture supernatants of El Tor CTXphi lysogens increased rapidly during exponential growth but

112 ambda lysogen with the generated lysate (the lysogen inhibits the helper phage used to package the re

113 Hence, if the repressor in a lysogen is present as a dimer, how can RecA-stimulated a

114 e high phage titer in cultures of Lula/phi80 lysogens is apparently in response to endogenous DNA dam

115 on Escherichia coli and bacteriophage lambda lysogens is reported.

116 pression-enhancing activity that the derived lysogens lack.

117 s are capable of detecting low numbers of L5 lysogens like L5 luciferase phages.

118 The lysogen-lytic viral reproduction switch is central to vi

119 disruptants grew more slowly than a control lysogen made with an att+ phage vector and gave smaller

120 e-encoded genes that exclusively benefit the lysogen maintain prophages at higher frequencies compare

121 Unlike wild-type lysogens, mutant lysogens were somewhat unstable under c

122 Why are lysogens not only resistant but also immune to the phage

123 Induction of a lysogen of a lambdoid bacteriophage usually involves Rec

124 Establishment and maintenance of a lysogen of the lambdoid bacteriophage 434 require that t

125 and virulence was reexamined by creating new lysogens of 158 and of a relatively avirulent mutant, st

126 Lysogens of Ef11 are resistant to the Ef11 endolysin.

127 nits of a Shiga-like toxin; Escherichia coli lysogens of H-19B are converted to toxin producers.

128 Lysogens of phage HK022 are resistant to infection by ph

129 We produced isogenic Escherichia coli K-12 lysogens of seven different Shiga toxin 2 (Stx2)-encodin

130 intestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to i

131 Lysogens of Stx phages are known to be less stable than

132 Pathogenic strains of Vibrio cholerae are lysogens of the filamentous phage CTXphi, which carries

133 We constructed lysogens of the seven B3-like phages in strain Ps33 of P

134 We found that the majority of lysogens offer protection against at least one additiona

135 xtension analysis of RNA isolated from HK022 lysogens or RNA made in vitro by transcribing a template

136 six sequences showed evidence of novel phage lysogens or sequence remnants of phage integrations, inc

137 were isolated from the environment and from lysogens, or were obtained from other laboratories.

138 favor the evolution of resistant and immune lysogens, particularly if the environment includes virul

139 expressed repressor explain why members of a lysogen population are spontaneously induced.

140 ne phosphorylation can be detected in a 933W lysogen prior to infection with HK97, while extensive St

141 We also demonstrate that lysogens produce signaling molecules and that signal is

142 pread in the laboratory environment: cryptic lysogen productivity and stealthy infectivity.

143 ically relevant context of an induced lambda lysogen, Q remains stably associated with RNAP as it tra

144 We found that dinL mutant lysogens release fewer phage in response to endogenous D

145 MAV1 DNA were cloned from three independent lysogens shown to have MAV1 DNA inserted at different si

146 As L5 lysogens spontaneously generate free phage particles, pr

147 a toxins by Stx phages is directly linked to lysogen stability because toxins are only synthesized an

148 Reduced lysogen stability can lead to increased frequency of gen

149 mbda), but this has a minimal effect on 933W lysogen stability.

150 repressor, the phenotype observed in B3-like lysogens suggested the presence of other exclusion genes

151 b protein, in sharp contrast to the parental lysogen, suggesting that ECOR-9 has an expression-enhanc

152 half of intestinal viruses are derived from lysogens, suggesting that these bacteria encounter trigg

153 of V. fischeri with the Aeromonas sp. ARM81 lysogen suppresses phage ARM81ld virion production.

154 et, restoring a functional cat gene; induced lysogens survive and are chloramphenicol resistant.

155 dolysin-dependent lysis of an induced lambda lysogen that was defective in the holin gene.

156 As a lysogen, the prophage alters the bacterial physiology by

157 In CTXphi lysogens, the activity of P(rstA), the only CTXphi promo

158 r prophage by immunity; where upon infecting lysogens, the free temperate phage coded by their propha

159 In bacteriophage lambda lysogens, the lambdacI repressor is encoded by the leade

160 Contrary to the lysogens, these capsulated-resistant clones are pan-resi

161 During passage of CTXphi lysogens through the infant mouse intestine, transductio

162 reporter system integrated as a single-copy lysogen to avoid titrating NtrC or polymerase.

163 uced Stx2d1 only, and supernatants from that lysogen transformed with a plasmid encoding RecA were cy

164 Lysogens underwent prophage induction upon addition of a

165 olling CI expression was weakened, rendering lysogens unstable.

166 n and viral replication are disassociated in lysogens until an induction event such as DNA damage occ

167 lysogens (cells harboring prophages) to non-lysogens varied widely associated with age, health condi

168 lasmid encoding RecA were cytotoxic when the lysogen was induced with mitomycin C.

169 Moreover, an stx(2d1)-containing lysogen was isolated from plaques on strain DH5alpha tha

170 bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken.

171 Lysogens were also transformed with these vectors, by vi

172 These lysogens were capable of transducing an E. coli recipien

173 chromosomal DNA junctions from each of three lysogens were determined.

174 When lambda(imm434) lysogens were grown to mid-log or stationary phase and s

175 Unlike wild-type lysogens, mutant lysogens were somewhat unstable under certain growth con

176 to 40-fold more toxin than a pure culture of lysogens, whereas the addition of phage to phage-resista

177 lytic infection, but did produce light in L5 lysogens which are known to repress D29 promoters.

178 ains a complex microbial ecosystem with many lysogens, which are bacteria containing dormant phages (

179 ion of E. coli to create a library of single lysogens, which were tested for defense against the same

180 Infection of the 933W lysogen with a non-excluded phage fails to induce Stk-de

181 ce difference, we further compared the TIGR4 lysogen with an equally transparent variant of TIGR4 in

182 ing a recombination-deficient E. coli lambda lysogen with the generated lysate (the lysogen inhibits

183 recognize an OcRNA sequence by selecting for lysogens with a P22 R17 [Oc] phage derivative.

184 beta-galactosidase production in single-copy lysogens with appropriate genotypes.

185 The subpopulation of STEC lysogens with induced prophages has been postulated to c

186 x encoding prophages are induced compared to lysogens with non-Stx encoding prophages, suggesting inc

187 (iii) A greater fraction of lysogens with stx encoding prophages are induced compare

188 the closed-open transition probability), the lysogen would be significantly less stable.