ライフサイエンスコーパス: mannosidase

1 se caused by a deficiency of the enzyme beta-mannosidase.

2 lternate pathway comprising a distinct alpha-mannosidase.

3 ral and mechanistic dissection of endo-alpha-mannosidase.

4 de hydrolase (GH) family (GH164) of putative mannosidases.

5 in turn induces expression of secreted alpha-mannosidases.

6 base of the barrel similar to other Class 1 mannosidases.

7 )S5 conformational itinerary for GH125 alpha-mannosidases.

8 2 epitope is lost when gp120 is treated with mannosidases.

9 eviously unobserved structural-fold for beta-mannosidases.

10 tity) to class I animal and fungal alpha-1,2 mannosidases.

11 GH130 mannoside phosphorylases and beta-1,2-mannosidases.

12 ic GM inhibitors not affecting the lysosomal mannosidases.

13 ed glycans, including an abundance of acidic mannosidases.

14 r human lysosomal and Drosophila Golgi alpha-mannosidases.

15 tions processed for in situ hybridization of mannosidase 1, alpha (CA1), bcl-2-related ovarian killer

16 rans-Golgi, respectively, using domains from mannosidase-1 and galactosyltransferase.

17 Beta-mannosidase, a lysosomal enzyme which acts exclusively a

18 ed previously for the core-specific alpha1,6-mannosidase (acidic pH optimum, inhibition by swainsonin

19 equires endo-1,4-beta-mannanase and 1,4-beta-mannosidase activities.

20 The production of mannosidase activity by all currently recognized species

21 Thus a nonspecific alpha-(1 --> 2)-mannosidase activity converts the glycoprotein to its Ma

22 ve subsequently demonstrated the presence of mannosidase activity in E. faecalis, which releases free

23 ologs, respectively, each of which had alpha-mannosidase activity in vitro.

24 uences caused by the lack of cytosolic alpha-mannosidase activity in vivo by the generation of Man2c1

25 le continuous assay for measurement of alpha-mannosidase activity is described and demonstrated for a

26 vidence support a model in which neither the mannosidase activity nor catalytic domain is essential f

27 When either mannosidase activity or the catalytic activity of protea

28 excellent substrate for the demonstration of mannosidase activity since it is a glycoprotein with a s

29 Intriguingly, EDEM mannosidase activity was dispensable for these protectiv

30 Mannosidase activity was produced only by some members o

31 Inhibition of mannosidase activity with kifunensine or disruption of t

32 s of this enzyme indicated that it has alpha-mannosidase activity, however, we conclusively show that

33 1-deoxymannojirimycin, an inhibitor of alpha-mannosidase activity, without affecting the monomer popu

34 omozygous mutant mice have undetectable beta-mannosidase activity.

35 rement for chitobiase action before alpha1,6-mannosidase activity.

36 cative of a novel post-endoplasmic reticulum mannosidase activity.

37 peroris and Streptococcus sanguinis produced mannosidase activity.

38 f a fusion between the first 13 exons in the mannosidase alpha class 2A member 1 gene (MAN2A1) and th

39 ownstream of EDEM1 (ER degradation enhancer, mannosidase alpha-like 1) (Pgenotype=0.042).

40 C), matrix metalloprotease-2, collagen-6-A1, mannosidase-alpha-1A and HLA-DPA1.

41 alpha-Mannosidase (Ams1) is another resident hydrolase that en

42 enzymes, aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p), to the vacuole.

43 ining the open reading frame of endo-alpha-D-mannosidase, an enzyme involved in early N-linked oligos

44 nd mannose branching glycans, and alpha1-2,3 mannosidase, an enzyme that cleaves 1-2 and 1-3 mannopyr

45 ene organization with tomato endosperm beta -mannosidase and barley seed beta -glucosidase/ beta -man

46 however, this enzyme also exhibited 1,4-beta-mannosidase and cellodextrinase activities.

47 ere constructed to localize active alpha-1,2-mannosidase and human beta-1,2-N-acetylglucosaminyltrans

48 omal glycosidases alpha-galactosidase, alpha-mannosidase and neuraminidase.

49 the G protein with the exoglycosidase alpha-mannosidase and reduced after subsequent treatment with

50 f the antigen oligomannoside moiety by alpha-mannosidase and that CD1e is an accessory protein absolu

51 rly been used for identifying beta-(1 --> 4)-mannosidase and the derived Man(0) form has served in tu

52 talytic properties of the Sf9 class II alpha-mannosidase and to more clearly determine its relationsh

53 alpha-Mannosidases and alpha-mannanases have attracted attenti

54 Endo-alpha-1,2-mannosidases and endo-alpha-1,2-mannanases, members of g

55 ries encoding catalytic domains of alpha-1,2-mannosidases and GnTI from mammals, insects, amphibians,

56 n), core-specific alpha1,6-mannosidase, beta-mannosidase, and cleavage at the reducing terminus by a

57 fluorescent protein-tagged soybean alpha-1,2 mannosidase, and correlated the findings to cytoskeletal

58 mmalian and yeast oligosaccharide-processing mannosidases, and the full-length coding region of the p

59 Based on the mannosidase- and lactacystin-sensitive properties of int

60 ymatic activities and joint actions of these mannosidases are required for this antiviral activity.

61 Although ubiquitously expressed, alpha-mannosidases are targeted to lysosomes or vacuoles throu

62 e cargo proteins, aminopeptidase I and alpha-mannosidase, are selectively transported from the cytopl

63 gh lvsB mutants inefficiently retained alpha-mannosidase, as well as two other lysosomal cysteine pro

64 This enzyme is not a lysosomal alpha-mannosidase because it is not active at acidic pH and it

65 catalytic itinerary is that of exo-1,6-alpha-mannosidases belonging to CAZy family 125.

66 mannosidase (LysMan), core-specific alpha1,6-mannosidase, beta-mannosidase, and cleavage at the reduc

67 ase and barley seed beta -glucosidase/ beta -mannosidase BGQ60.

68 .44 did not bind antigen treated with beta-D-mannosidase but did bind antigen treated with alpha-D-ma

69 nstrated that other mammalian class II alpha-mannosidases can participate in N-glycan processing.

70 Mannose liberated from the actions of mannosidases can, if desired, be quantified by, for exam

71 d residues map to the periphery of the alpha-mannosidase catalytic domain tertiary structure.

72 One unusual enzyme, endo-alpha-mannosidase, cleaves mannoside linkages internally withi

73 The mannosidase component guarantees docking into the Golgi

74 Class I alpha-mannosidases comprise a homologous and functionally dive

75 d, and in many studies, the numbers of alpha-mannosidase-containing cells were enumerated.

76 A beta-mannosidase could be known to hydrolyze beta-mannose, fo

77 CD1e selectively assists the alpha-mannosidase-dependent digestion of PIM(6) species accord

78 ssembly and release of class II is linked to mannosidase-dependent ERAD targeting of the misfolded Ii

79 nce tags were identified for bovine alpha1,6-mannosidase, despite the identification of two sequence

80 med using recombinant Drosophila Golgi alpha-mannosidase (dGMII) has been shown to give the kinetic p

81 ycobacterium smegmatis and digested by alpha-mannosidase, did not activate T cells.

82 Mannosidase digestion and concanavalin A adsorption indi

83 otease treatments but was destroyed by alpha-mannosidase digestion.

84 tion, we have identified three class I alpha-mannosidases, EDEM1, EDEM2, and ERManI, which play a cri

85 sidase, endo-(1-->4)-beta-D-xylanase, beta-D-mannosidase, endo-(1-->4)-beta-D-mannanase, alpha-D-xylo

86 Endoplasmic reticulum (ER) alpha-1, 2-mannosidase (ERManI) contributes to ER-associated protei

87 ies of mammalian Class 1 processing alpha1,2-mannosidases (family 47 glycosidases) play critical role

88 1p (Htm1p-Pdi1p) acts as a folding-sensitive mannosidase for catalyzing this first committed step in

89 m temperature of LAM-spiked urine with alpha-mannosidase (for human TB), and LAM-spiked milk with com

90 ed activity-based protein profiling of alpha-mannosidases from both human cell lysate and mouse tissu

91 and the biologically relevant class II alpha-mannosidases from Drosophila melanogaster belonging to g

92 lts suggest that the chitobiase and alpha1,6-mannosidase function in tandem for mammalian lysosomal N

93 lying these traits as well as a single alpha-mannosidase gene directly associated with this tolerance

94 d the entire coding region of the human beta-mannosidase gene using a combination of cDNA library scr

95 '-flanking sequences for the bovine alpha1,6-mannosidase genes may lead to defective transcription si

96 uscle cell line C2, permanently expressing a mannosidase-green fluorescent protein (GFP) construct.

97 showed good and selective inhibition of beta-mannosidase (Helix pomatia).

98 epitope-tagged full-length form of the human mannosidase homolog in normal rat kidney cells resulted

99 he full-length coding region of the putative mannosidase homolog was isolated by a combination of 5'-

100 he COOH-terminal luminal domain of the human mannosidase homolog, was expressed in COS cells, the fus

101 Here we report that the complex of the mannosidase Htm1p and the protein disulfide isomerase Pd

102 ibition of human endoplasmic reticulum alpha-mannosidase I (ER Man I) and mouse Golgi alpha-mannosida

103 , including endoplasmic reticulum (ER) alpha-mannosidase I (ERManI) and Golgi alpha-mannosidase IA (G

104 d how, modification by endoplasmic reticulum mannosidase I (ERManI) contributes to the preferential s

105 ER mannosidase I (ERManI) is a quality control factor that

106 Endoplasmic reticulum alpha1,2 mannosidase I (ERManI), a central component of ER qualit

107 ene of Drosophila melanogaster encodes Golgi mannosidase I (MAS-1), and flies homozygous for small de

108 d by the sequential action of Golgi alpha1,2-mannosidase I (MIa,b,c), MGAT1, alpha1,2-mannosidase II

109 The Arabidopsis ER-alpha-mannosidase I (MNS3) generates an oligomannosidic N-glyc

110 Interestingly, inhibition of mannosidase I also results in prolonged association betw

111 lycoprotein processing inhibitors that block mannosidase I and increase the amount of protein-bound M

112 me reactions using a combination of human ER mannosidase I and recombinant Golgi mannosidase IA indic

113 proteins as well as vesicle cargo molecules (mannosidase I and sialyltransferase-yellow fluorescent p

114 selective trimming of N-glycans by ER alpha-mannosidase I and subsequent recognition by the ER degra

115 ached to misfolded glycoproteins by ER alpha-mannosidase I and subsequent recognition by the ER degra

116 wild type and mutant forms of human ER alpha-mannosidase I as well as by structural analysis of a co-

117 erized by down-regulation of the Golgi alpha-mannosidase I coding gene MAN1A1, leading to elevation o

118 teolytically driven checkpoint control of ER mannosidase I contributes to the establishment of an equ

119 alnexin (CNX) and calreticulin (CRT), and ER mannosidase I in apo(a) intracellular targeting.

120 g the participation of endoplasmic reticulum mannosidase I in the disposal process.

121 rent primary cells or in the presence of the mannosidase I inhibitor deoxymannojirimycin dramatically

122 In addition, ER mannosidase I inhibitor kifunensine and down-regulation

123 ral serine kinase inhibition implied that ER mannosidase I is subjected to regulated proteolysis.

124 Unexpectedly, mannosidase I redistributed from the Golgi complex to co

125 reshaping of the glycome by inhibiting alpha-mannosidase I resulted in significantly higher migratory

126 endent on mannose trimming and inhibition of mannosidase I stabilizes Ii.

127 igosaccharides by endoplasmic reticulum (ER) mannosidase I targets misfolded glycoproteins for disloc

128 Redistribution of mannosidase I was also observed in cells incubated at 15

129 Herein the intracellular fate of human ER mannosidase I was monitored to determine whether a post-

130 Inhibition of ER mannosidase I with deoxymannojirimycin or kifunensine ha

131 MAN1B1 gene product MAN1B1, also known as ER mannosidase I, is to function within the ER similar to t

132 abidopsis (Arabidopsis thaliana) Golgi alpha-mannosidase I, Nicotiana tabacum beta1,2-N-acetylglucosa

133 haride modification by endoplasmic reticulum mannosidase I, the latter of which occurred as PI Z was

134 sly isolated for Saccharomyces cerevisiae ER mannosidase I, the oligosaccharide in the active site of

135 in which processing by endoplasmic reticulum mannosidase I, which attenuates the removal of glucose f

136 wild type and mutant forms of human ER alpha-mannosidase I.

137 ncoding the resident Golgi protein alpha-1,2 mannosidase I.

138 e encodes an enzyme previously designated ER mannosidase I.

139 Remarkably, ER mannosidase I/Man1b1, the first alpha-mannosidase implic

140 g five active eukaryotic proteins, including mannosidases I and II, N-acetylglucosaminyl transferases

141 cells cultured in the presence of the alpha-mannosidase-I inhibitor kifunensine.

142 alpha-mannosidase I (ERManI) and Golgi alpha-mannosidase IA (GMIA), are responsible for cleavage of t

143 nnosidase I (ER Man I) and mouse Golgi alpha-mannosidase IA (Golgi Man IA).

144 nosidases, we have crystallized murine Golgi mannosidase IA (space group P2(1)2(1)2(1)), and the stru

145 human ER mannosidase I and recombinant Golgi mannosidase IA indicated that that these two enzymes are

146 t yeast strain and subsequent treatment with mannosidase IA.

147 nnose trimming enzyme drosophila Golgi alpha-mannosidase II (dGMII) complexed with the inhibitors man

148 characterized included inserts in the alpha-mannosidase II (dGMII), ash1, and pumilio genes.

149 Golgi mannosidase II (GMII) catalyzes the sequential hydrolysi

150 Golgi alpha-mannosidase II (GMII), a member of glycoside hydrolase f

151 aminyltransferase I, Arabidopsis Golgi alpha-mannosidase II (GMII), and Arabidopsis beta1,2-xylosyltr

152 Inhibition of Golgi alpha-mannosidase II (GMII), which acts late in the N-glycan p

153 nd cosedimented with the Golgi marker, alpha-mannosidase II (Man II).

154 GalT was compared with transfected rat alpha-mannosidase II (medial-Golgi, polyclonal antibody).

155 alpha-Mannosidase II (MII) is a key enzyme converting precurso

156 1,2-mannosidase I (MIa,b,c), MGAT1, alpha1,2-mannosidase II (MII, IIx), and MGAT2.

157 development consistent with increasing alpha-mannosidase II and core fucosyl-transferase enzyme activ

158 cDNA encoding a protein homologous to alpha-mannosidase II and designated it alpha-mannosidase IIx.

159 Mannosidase II and giantin were observed to colocalize w

160 reen et al. now show that mice lacking alpha-mannosidase II develop an autoimmune disease similar to

161 alpha-mannosidase IIx colocalizes with alpha-mannosidase II in COS cells.

162 The functional role of ER mannosidase II in glycoprotein quality control is discus

163 Golgi alpha-mannosidase II is an enzyme that processes the intermedi

164 nto small punctate structures at a time when mannosidase II is still largely localized to Golgi struc

165 g kinetics was seen with the HeLa GalT/alpha-mannosidase II pairing.

166 ed oligosaccharides by endoplasmic reticulum mannosidase II partitions variant PI Z away from the con

167 on is temporally and spatially distinct from mannosidase II relocation and that FTCD provides a novel

168 its redistribution is distinct from that of mannosidase II relocation.

169 onsisting of the first 117 residues of alpha-mannosidase II tagged with a fluorescent protein and a t

170 Swainsonine, an inhibitor of Golgi alpha-mannosidase II that causes abnormal N-glycosylation, str

171 taining pattern was similar to that of alpha-mannosidase II which is a known resident enzyme of the G

172 NBCCV was found to be colocalized with alpha-mannosidase II, a marker for the Golgi complex.

173 noprecipitate betaCOP, Golgi 58K protein, or mannosidase II, all Golgi-resident proteins.

174 When coexpressed with alpha-mannosidase II, alpha-mannosidase IIx colocalizes with a

175 -GFP fusion colocalized with a Golgi marker, mannosidase II, and retained catalytic activity compared

176 Swainsonine, an inhibitor of Golgi alpha-mannosidase II, blocked beta1,6GlcNAc N-glycan expressio

177 tegral membrane Golgi proteins called GEARs (mannosidase II, GOS-28, GS15, GPP130, CASP, giantin, and

178 several Golgi and vesicle markers, including mannosidase II, p58, trans-Golgi network (TGN)38, and be

179 at mutation of a single gene, encoding alpha-mannosidase II, which regulates the hybrid to complex br

180 icular stomatitis virus (VSV)-G protein to a mannosidase II-containing Golgi compartment.

181 d for the delivery of a cargo protein to the mannosidase II-containing Golgi compartment.

182 alpha-Mannosidase II-deficient autoimmune disease is due to an

183 ne its relationship to mammalian Golgi alpha-mannosidase II.

184 localized with the medial-Golgi marker alpha-mannosidase II.

185 emical similarities to mammalian Golgi alpha-mannosidase II.

186 the Sf9 enzyme is distinct from Golgi alpha-mannosidase II.

187 es originating from the Golgi and containing mannosidase II.

188 Alpha-mannosidase-II (alphaM-II) deficiency diminishes complex

189 olgi transport of Rh1, downstream from alpha-mannosidase-II in the medial- Golgi.

190 Brefeldin A treatment inhibited mannosidase-II recruitment and phagocytic uptake of seru

191 Mechanistically, recruitment of mannosidase-II vesicles during phagocytic uptake require

192 The recruitment of mannosidase-II vesicles was an early event mediated by f

193 We report recruitment of mannosidase-II-positive Golgi-derived vesicles during up

194 perties, we designated this enzyme Sf9 alpha-mannosidase III and concluded that it probably provides

195 Because alpha-mannosidase IIx (MX) is a candidate enzyme for this path

196 atozoa, a phenotype similar to that of alpha-mannosidase IIx (MX) KO mice.

197 disruption of Man2a2, a gene encoding alpha-mannosidase IIx (MX), an enzyme that forms intermediate

198 coexpressed with alpha-mannosidase II, alpha-mannosidase IIx colocalizes with alpha-mannosidase II in

199 in A fusion of the catalytic domain of alpha-mannosidase IIx hydrolyzes a synthetic substrate, 4-umbe

200 The results suggest that alpha-mannosidase IIx hydrolyzes two peripheral Man alpha 1-->

201 e, we show by immunocytochemistry that alpha-mannosidase IIx resides in the Golgi in HeLa cells.

202 ese hamster ovary cells overexpressing alpha-mannosidase IIx show a reduction of M(6)Gn(2) and an acc

203 alpha-mannosidase II and designated it alpha-mannosidase IIx.

204 ly, ER mannosidase I/Man1b1, the first alpha-mannosidase implicated in this conventional N-glycan-med

205 d with kifunensine, an inhibitor of alpha1,2-mannosidase in the ER, indicating that degradation of AT

206 analysis demonstrates that BtMan2A is a beta-mannosidase in which substrate binding energy is provide

207 function for maternally deposited acid alpha-mannosidase in yolk consumption.

208 pha- and beta-galactosidase, alpha- and beta-mannosidase) in an assay that measured the rate of hydro

209 e glycoside hydrolase family 47 (GH47) alpha-mannosidases, including endoplasmic reticulum (ER) alpha

210 m in which Golgi-localized MAN1B1 can play a mannosidase-independent gatekeeper role in the proteosta

211 Accelerated degradation occurred in a mannosidase-independent manner and was arrested by lacta

212 al content of the beta subunit was less with mannosidase inhibition compared with that found in the N

213 Proteasome inhibition but not mannosidase inhibition led to the accumulation of full-l

214 Golgi mannosidase inhibition to prevent carbohydrate chain bra

215 hibitor lactacystin, and in combination with mannosidase inhibition, revealed that the removal of man

216 Complexes with the established endo-alpha-mannosidase inhibitor alpha-Glc-1,3-deoxymannonojirimyci

217 iated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutrali

218 ctical synthesis of the potent class I alpha-mannosidase inhibitor kifunensine (1) beginning from the

219 hen Env was expressed in the presence of the mannosidase inhibitor kifunensine to force retention of

220 Mice treated with the mannosidase inhibitor kifunensine to prevent the formati

221 a CHO cell line in the presence of an alpha-mannosidase inhibitor kifunensine, and an endoglycosidas

222 pe KOR1 in the presence of the class I alpha-mannosidase inhibitor kifunensine, which abolished the c

223 ng several concentrations of the known alpha-mannosidase inhibitor swainsonine are also presented, de

224 reatment of zebrafish embryos with the alpha-mannosidase inhibitor swainsonine resulted in the accumu

225 Finally, kifunensine, a mannosidase inhibitor that can block entry of ER protein

226 er of alpha-mannosides, and the potential of mannosidase inhibitors as cellular probes and therapeuti

227 Compounds 15 and 16 were specific alpha-mannosidase inhibitors, and 24 and 26 were potent and se

228 se inhibitor, castanospermine (CST), and two mannosidase inhibitors, kifunensine (KIF) and deoxymanno

229 sidase, that is susceptible to several known mannosidase inhibitors.

230 substrate interactions within the family 47 mannosidases involved in glycan maturation and ER-associ

231 f glycosidases including the Jack Bean alpha-mannosidase (JBalphaMan) and the biologically relevant c

232 ial treatment strategies, we produced a beta-mannosidase knockout mouse.

233 By contrast, the lysosomal mannosidase lacks an equivalent GlcNAc binding site and

234 the majority of strains transformed with the mannosidase/leader library displayed only modest in vivo

235 clinically and pathologically, tissue alpha-mannosidase levels were assayed, and in many studies, th

236 EDEM1 (ER degradation-enhancing alpha-mannosidase-like 1 protein) has been proposed to play a

237 with kifunensine or disruption of the EDEM1 mannosidase-like domain by mutation had no effect on EDE

238 EDEM1 binds nonnative proteins and uses its mannosidase-like domain to target aberrant proteins to t

239 1 associates through a region outside of its mannosidase-like domain with the nonglycosylated protein

240 d upregulates ER degradation-enhancing alpha-mannosidase-like protein (EDEM) and ER chaperones, thus

241 mes predicted ER degradation-enhancing alpha-mannosidase-like protein and Mns1 orthologs, respectivel

242 nition by the ER degradation-enhancing alpha-mannosidase-like protein family of lectins, both members

243 nition by the ER degradation-enhancing alpha-mannosidase-like protein family of lectins, both members

244 lasmic reticulum degradation-enhancing alpha-mannosidase-like protein mRNA levels were inversely rela

245 tion of EDEM (ER degradation-enhancing alpha-mannosidase-like protein) also suppressed the degradatio

246 tion of EDEM (ER degradation-enhancing alpha-mannosidase-like protein).

247 et gene EDEM (ER degradation-enhancing alpha-mannosidase-like protein, a protein degradation factor)

248 luding ERdj4, ER degradation-enhancing alpha-mannosidase-like protein, and p58(IPK), or expression of

249 through upregulation of ERAD-enhancing alpha-mannosidase-like proteins (EDEMs) protected against chro

250 atively, an alpha-1,2-mannosidase (Mns1) and mannosidase-like proteins (ER degradation-enhancing alph

251 ike proteins (ER degradation-enhancing alpha-mannosidase-like proteins 1, 2, and 3) are part of a pro

252 -mannosidase ManIIb (GM) and lysosomal alpha-mannosidase LManII (LM).

253 ncluding a broad specificity lysosomal alpha-mannosidase (LysMan), core-specific alpha1,6-mannosidase

254 ycan processing gene arrays identified alpha-mannosidases (MAN1A2 and MAN1C1) as targets for down-reg

255 (Nicotiana tabacum) plants of a human alpha-mannosidase, MAN2B1, which is a lysosomal enzyme involve

256 alpha-Mannosidase (MAN2C1) is the enzyme responsible for the p

257 to one of the target genes, lysosomal beta A mannosidase (MANBA), we observed that genetic variants a

258 Endo-alpha-1,2-mannosidase (MANEA) is the sole endo-acting glycoside hy

259 glycohydrolase family 38, namely Golgi alpha-mannosidase ManIIb (GM) and lysosomal alpha-mannosidase

260 ng function, and that genes related to alpha-mannosidase may influence risk of emphysema.

261 Alternatively, an alpha-1,2-mannosidase (Mns1) and mannosidase-like proteins (ER deg

262 e role of Arabidopsis thaliana class I alpha-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.

263 f the beta-1,2-xylose, followed by the alpha-mannosidase NixJ (GH125), which removes the alpha-1,6-ma

264 emoval of the alpha-1,3-mannose by the alpha-mannosidase NixK (GH92) is a prerequisite for the subseq

265 lines processed and targeted lysosomal alpha-mannosidase normally, indicating the lack of a significa

266 anavalin A as well as the enzymes alpha1-2,3 mannosidase or beta1-4 galactosidase to provide structur

267 se but did bind antigen treated with alpha-D-mannosidase, other alpha- or beta-glycosidases, or a pan

268 ine-linked oligosaccharides by a slow-acting mannosidase partitions the misfolded monomer into the pr

269 ERManI, a putative ER resident mannosidase, plays a rate-limiting role in generating a

270 ervation that this motif is invariant in GH2 mannosidases points to a generic role for these residues

271 we determine that most glucosidases and beta-mannosidases preferentially bind their substrates in the

272 ides in the ER lumen, followed by ENGase and mannosidase processing in the cytosol and lysosomes.

273 Investigations on mannosidase production using synthetic (4-methylumbellif

274 ycoprotein as the substrate demonstrate that mannosidase production within the viridans group strepto

275 Digestion with mannosidase reduced infectivity by fivefold.

276 us for upper-lower lobe ratio near the alpha-mannosidase-related gene MAN2B1 (rs10411619; P = 1.1 x 1

277 frican Americans, a locus near a third alpha-mannosidase-related gene, MAN1C1 (rs12130495; P = 9.9 x

278 ent of the catalytic domain of class I alpha-mannosidases reveals four well-supported phylogenetic gr

279 he reaction coordinate of an inverting alpha-mannosidase show how the enzyme distorts the substrate a

280 ence, such as the neuraminidase NanA and the mannosidase SpGH92, we anticipate that the alpha-fucosid

281 o be less potent inhibitors of Class I alpha-mannosidases than kifuensine itself, the bis(hydroxymeth

282 length cDNA clone encoding a human alpha1, 2-mannosidase that catalyzes the first mannose trimming st

283 that Htm1p-Pdi1p is a glycoprotein-specific mannosidase that preferentially targets nonnative glycop

284 rom all other known mammalian class II alpha-mannosidases that can hydrolyze Man(5)GlcNAc(2).

285 phosphate binding residues, are indeed beta-mannosidases that hydrolyze beta-1,2-mannosidic linkages

286 e found that it is a trimeric retaining beta-mannosidase, that is susceptible to several known mannos

287 tions of alpha-(1 --> 3) and alpha-(1 --> 6)-mannosidases, to the Man(1) form via Man(4), Man(3), and

288 alpha1,6-Mannosidase transcripts were ubiquitously expressed in h

289 A swainsonine-sensitive alpha-mannosidase trims some N-glycans to biantennary Man(3)Gl

290 However, no evidence of a role for mannosidases was found for TCR-alpha, and significant re

291 asis for substrate recognition among Class 1 mannosidases, we have crystallized murine Golgi mannosid

292 ing efficiency of the lysosomal enzyme alpha-mannosidase were normal in the mutant strain.

293 e efficiency of targeting of lysosomal alpha-mannosidase were normal, although lvsB mutants inefficie

294 Three class I alpha-mannosidases were identified to play a critical role in

295 eriments, Rab7 T22N cells oversecreted alpha-mannosidase, whereas Rab7 WT cells retained this hydrola

296 (GFP) fusion protein co-localized with alpha-mannosidase, which indicated that the fusion protein loc

297 multiple enzymatic digestion steps including mannosidase with activity toward specific Man(alpha 1,3)

298 ct (Sf9) cell cDNA encoding a class II alpha-mannosidase with amino acid sequence and biochemical sim

299 ovel human glycosylhydrolase family 38 alpha-mannosidase with catalytic characteristics similar to th

300 city studies comparing the novel human alpha-mannosidase with human LysMan revealed that the former e