ライフサイエンスコーパス: mannosylation

1 onal description of MIR domains in protein O-mannosylation.

2 hown to catalyze the initial step of protein mannosylation.

3 Kdo residue of Kdo2-lipid IVA as the site of mannosylation.

4 the Golgi from the cytoplasm is required for mannosylation.

5 orylceramide due to a defect in sphingolipid mannosylation.

6 in effect was enriched for genes involved in mannosylation.

7 hat PTP69D is a substrate of POMT-mediated O-mannosylation.

8 ry amino acid selectivity near the site of O-mannosylation.

9 cted links to calcium signalling and protein mannosylation.

10 ences between co- and post-translocational O-mannosylation.

11 SL2 TSRs also have consensus sequences for C-mannosylation.

12 xtensive hydrogen bonding network, including mannosylation.

13 lycan synthesis by disrupting dystroglycan O-mannosylation.

14 if is known to be a consensus sequence for C-mannosylation.

15 phosphatidylinositol-anchoring, and C- and O-mannosylation.

16 -branching, core 1 O-glycan formation, and O mannosylation.

17 a1-6-D-myo-inositol-1-HPO4-sn-lipid precedes mannosylation.

18 to catalyze the next two sequential alpha1,2-mannosylations.

19 normal brain development requires protein O-mannosylation activity in neural tissue but not the meni

20 vrg4 mutation causes a general impairment in mannosylation, affecting N-linked and O-linked glycoprot

21 O-Mannosylation also increases the thermostability of CBM

22 To investigate the role played by mannosylation, an immunoreactive cryptococcal mannoprote

23 Additionally, beta-mannosylation and 1,2-cis-d-FucNAc linkage formations we

24 degrees of microheterogeneity; 9 sites of O-mannosylation and 14 sites of O-GalNAcylation were obser

25 yl-PI accumulation would also be expected if mannosylation and acylation were independent of each oth

26 that Mnt1 and Mnt2 are involved in O-linked mannosylation and are required for virulence.

27 iative S(N)2-like mechanism, whereas alpha-O-mannosylation and beta-C-mannosylation are dissociative

28 these unusual sugar modifications, protein O mannosylation and glycan modifications involving the put

29 that the alpha1,6-, alpha1,2-, and alpha1,3-mannosylation and Kex2p-dependent processing events mark

30 as indicated that the alpha1,6- and alpha1,3-mannosylation and Kex2p-dependent processing of pro-alph

31 ein is a significant substrate for protein O-mannosylation and led to the identification of several n

32 branching and elongation as well as elevated mannosylation and N-glycan truncation in AD.

33 O-Mannosylation and N-glycosylation are essential protein

34 ins of fungi are modified by N- and O-linked mannosylation and phosphomannosylation, resulting in cha

35 evolutionary relationship between protein O-mannosylation and protein N-glycosylation.

36 Moreover, the degree of total IgM mannosylation and sialylation correlate significantly wi

37 earch includes triflate-mediated direct beta-mannosylation and tandem glycosylation in a one-pot stra

38 P1 suggested that it is modified by O-linked mannosylation, and ConA binds to these O-linked mannose

39 s, particularly with respect to sialylation, mannosylation, and fucosylation, in normal, pancreatitis

40 tive glycosyltransferase genes involved in O-mannosylation are associated with a loss of ligand-bindi

41 putative glycosyltransferases involved in O-mannosylation are causal for various forms of congenital

42 sm, whereas alpha-O-mannosylation and beta-C-mannosylation are dissociative and S(N)1-like.

43 Here, the effects of O-mannosylation are examined on the Family 1 CBM from the

44 The biological functions of tryptophan C-mannosylation are poorly understood, in part, due to a d

45 Additional functions of O-mannosylation are still largely unknown.

46 eldin A-treated cells received only alpha1,6-mannosylation as did approximately 50% of pro-alphaf tra

47 and suggest that in the absence of proper O-mannosylation, as is associated with certain forms of mu

48 athies, result from defects in the protein O-mannosylation biosynthetic pathway.

49 , and CaMnt5 did not participate in O-linked mannosylation, but CaMnt3 and CaMnt5 had redundant activ

50 is acyl-CoA-dependent and takes place before mannosylation, but uniquely for this class of inositol-a

51 Mannosylation by DPY19L1 but not DPY19L3 is required for

52 canopathy syndrome, supported by deficient O-mannosylation by muscle immunohistochemistry.

53 BCR-associated mannosylation can be found in a subset of germinal cente

54 viding proof of principle that yeast-derived mannosylation can enhance immunogenicity.

55 te-directed mutagenesis, and expression in C-mannosylation-defective Chinese hamster ovary cell varia

56 partial loss of CaVRG4 function resulted in mannosylation defects, which in turn led to a number of

57 O-mannosylation-deficient embryos failed to proceed from t

58 The results show that, although CBM mannosylation does not induce major conformational chang

59 olecular and genetic mechanisms of protein O-mannosylation during development.

60 ant part of this glycosylation is a unique O-mannosylation, essential for the interaction of alpha-dy

61 this enzyme actually carries out an alpha1,3-mannosylation, followed by an alpha1,6-mannosylation, to

62 revious studies demonstrated importance of C-mannosylation for efficient protein secretion.

63 gyl ether in the benzylidene acetal directed mannosylation has a detrimental effect on stereoselectiv

64 In recent years protein O-mannosylation has become a focus of attention as a patho

65 Loss of protein O-mannosylation in both mutant strains was unambiguously d

66 it was suggested that acylation must precede mannosylation in both yeast and rodent cells because Glc

67 To study the roles of O-mannosylation in brain development we generated a condit

68 amily participates in three types of protein mannosylation in C. albicans, and these modifications pl

69 To begin to study the role of Golgi mannosylation in C. albicans, we isolated the CaVRG4 gen

70 monstrate that TSR1 from punctin-1 carries C-mannosylation in close proximity to O-linked fucose.

71 Our study demonstrates that regulation of O-mannosylation in higher eukaryotes is more complex than

72 Our results redefine the significance of O-mannosylation in humans and other mammals, showing the i

73 rprisingly, SL15 also corrects the defective mannosylation in Lec35 cells.

74 cation processes were required for protein O-mannosylation in M. tuberculosis.

75 of the biological significance of protein O-mannosylation in mycobacteria and demonstrate the crucia

76 ding of physiology and biochemistry of Trp N-mannosylation in proteins and the overall biochemical me

77 e earlier suggestion that acylation precedes mannosylation in rodents cells.

78 Here, we reinvestigate protein O-mannosylation in the context of protein translocation.

79 rate that inositol acylation is required for mannosylation in the HeLa cell GPI biosynthetic pathway,

80 e thus analyzed the impact of interrupting O-mannosylation in the nonpathogenic saprophyte Mycobacter

81 hat differentially expressed N- and O-linked mannosylation in the yeast Pichia pastoris and compared

82 However, the functional importance of O-mannosylation in these tissues at later stages remains l

83 o provide N-linked and/or extensive O-linked mannosylation increased the capacity of the model Ag OVA

84 C-Mannosylation is a common modification of thrombospondin

85 Protein O-mannosylation is a glycan modification that is required

86 eramides [GIPCs]) contains Man and that this mannosylation is affected in gonst1.

87 Protein O-mannosylation is an essential and evolutionarily conserv

88 Protein O-mannosylation is an essential post-translational modific

89 Among them, O-mannosylation is an unusual type of protein glycosylatio

90 e GlcNalpha-acyl-PI accumulates in vivo when mannosylation is blocked.

91 Thus, the process of protein mannosylation is conserved between M. tuberculosis and e

92 Thus, mannosylation is critical for optimal T cell responses t

93 Protein O-mannosylation is found in yeast and metazoans, and a fam

94 dependent upon the POMGnT1 enzyme and that O-mannosylation is not limited solely to alpha-DG in the b

95 e for RP and indicates that proper protein O-mannosylation is not only essential for early organ deve

96 Together, our data suggested that protein O-mannosylation is required for normal sensory feedback to

97 Protein C-mannosylation is the attachment of alpha-mannopyranose t

98 ransport (and hence Golgi apparatus-specific mannosylation) is a fungus-specific process.

99 Defects in protein O-mannosylation lead to severe congenital muscular dystrop

100 st to the highly conserved requirement for O mannosylation, more generic O glycans present on alpha-D

101 insights gained from the characterization of mannosylation mutants into the role of these cell wall c

102 erated in vitro by pure LpcC showed that the mannosylation occurs on the inner Kdo residue of Kdo(2)-

103 tero)aryl bromides, thereby enabling the a-C-mannosylation of 2-bromo-tryptophan, peptides thereof, a

104 he presence and functional significance of C-mannosylation of ADAMTS-like 1/punctin-1, which contains

105 usly shown for LCMV, we found that protein O mannosylation of alpha-DG is crucial for the binding of

106 sferase, POMGnT1, which is involved in the O-mannosylation of alpha-dystroglycan.

107 Surprisingly, O-mannosylation of cadherins and protocadherins does not r

108 used to determine that TMTC3 supports the O-mannosylation of E-cadherin, cellular adhesion, and embr

109 In contrast, it has been reported that mannosylation of endogenous GlcNalpha-PI by Trypansoma b

110 hese results suggest that a complete lack of mannosylation of glycoproteins in the Golgi leads to inv

111 lly reduces transcription of PIGM and blocks mannosylation of GPI, leading to partial but severe defi

112 t disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout i

113 n pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions d

114 Mannosylation of Kdo2-lipid IVA catalyzed by RfaC procee

115 r Lec35p was required only for MPD-dependent mannosylation of LLO and glycosylphosphatidylinositol in

116 +) T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded

117 Synthetic mannosylation of native allergens prevented antibody-med

118 Here, we present evidence that alpha1,2-mannosylation of pro-alphaf is also initiated in a disti

119 osphatidylinositol membrane anchoring, and O-mannosylation of protein.

120 The substrate for mannosylation of proteins and lipids in the Golgi appara

121 an-P-Dol-mediated reactions in N-, O-, and C-mannosylation of proteins, GPI anchor assembly, and the

122 GDP-mannose is required for O-mannosylation of proteins, including alpha-DG, and it is

123 , consistent with an interaction between the mannosylation of PTP1 and some unknown host cell mannose

124 These data suggest that the O-mannosylation of PTP1 may have functional significance f

125 ed glycoprotein modifications as well as the mannosylation of sphingolipids.

126 has a beta-hairpin insertion required for O-mannosylation of substrates.

127 in yeast that catalyzes the first step in O mannosylation of target proteins.

128 yl residues, we show that alterations in the mannosylation of the C. albicans cell wall affect the in

129 -12 mannosyl residues followed by additional mannosylation of the core and arabinosylation of a singl

130 Tases) are expressed in E. coli and used for mannosylation of the dolichol mimic, phytanyl pyrophosph

131 e palmitoylation of the inositol residue and mannosylation of the glucosamine residue of the glucosam

132 l-PI(C8) could be mannosylated in vitro, but mannosylation of the latter was significantly more effic

133 its TSR domain, and obtained evidence that C-mannosylation of the TSR influences LPS binding.

134 defects of smp3 mutants and permits in vivo mannosylation of trimannosyl (Man(3))-GPIs.

135 port that provides definitive evidence for N-mannosylation of Trp residues in a protein.

136 ructure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threon

137 reactions were investigated: MPD-dependent C-mannosylation of tryptophanyl residues, and glucose-P-do

138 C-Mannosylation of TSR1 of the related protease ADAMTS5 wa

139 ned out that prolonged ER residence allows O-mannosylation of un-/misfolded proteins or slow folding

140 reo- and site-selective galactosylations and mannosylations of a wide assortment of polyfunctional nu

141 h, we also performed late-stage tryptophan C-mannosylation on a diverse array of peptides, demonstrat

142 and suggests that the impact of changes in O-mannosylation on N-glycosylation has been underestimated

143 fect cell wall integrity, changes in surface mannosylation or the provision of additional carbon sour

144 and capsule production, stress responses, O-mannosylation, or retromer function.

145 tudy identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O

146 Disruption of the O-mannosylation pathway involved in functional glycosylati

147 lassical and evolutionarily conserved POMT O-mannosylation pathway is essentially dedicated to alpha-

148 tently, mutations in genes involved in the O-mannosylation pathway result in infantile-onset, severe

149 1, which encodes an essential component in O-mannosylation pathway, in three unrelated families with

150 Mannosylation patterns were mimicked by FL Ig-derived si

151 ent knock-out mouse models associated with O-mannosylation (POMGnT1, LARGE (Myd), and DAG1(-/-)).

152 used by mutations in two genes involved in O-mannosylation, POMT1 and POMGnT1, respectively.

153 ne are interpreted as indicating that beta-O-mannosylation proceeds via an associative S(N)2-like mec

154 and a few other proteins, whereas a novel O-mannosylation process in mammalian cells is predicted to

155 A posttranslational protein O-mannosylation process resembling that found in fungi and

156 Altered mannosylation profile was restored in patient cells upon

157 Since defects in the O-mannosylation protein glycosylation pathway are primaril

158 For the 4,6-O-benzylidene-directed mannosylation reaction a significant difference in conce

159 n the 4,6-O-benzylidene acetal directed beta-mannosylation reaction.

160 tivity of sensory neurons, suggesting that O-mannosylation regulates the sensory feedback controlling

161 Ag mannosylation represents a promising strategy to augment

162 es for protein N-glycosylation and protein O-mannosylation, respectively.

163 rexpression of LARGE in cells deficient in O mannosylation resulted in highly glycosylated alpha-DG t

164 The structures indicate how the C-mannosylation sequon is recognized by this CMT and its p

165 C-mannosylation sites were identified in TSP1, linker TSP4

166 ich contains four TSRs (two with predicted C-mannosylation sites), using mass spectrometry, metabolic

167 , which led to the identification of novel C-mannosylation sites.

168 ites and variable mannose stoichiometry at C-mannosylation sites.

169 The distribution of identified sites of O-mannosylation suggests a limited role for local primary

170 sive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.

171 hed a microsomal translation/translocation/O-mannosylation system.

172 urther demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and tr

173 on its apparent molecular mass of 98 kDa and mannosylation, the antigen of interest was named MP98.

174 pathway related to the yeast-type protein O-mannosylation, the enzymatic basis and functional import

175 ha1,3-mannosylation, followed by an alpha1,6-mannosylation, to form the first branched pentasaccharid

176 In absence of C-mannosylation, UNC-5 TSRs could only be obtained at low

177 ing to muscle cell glycoproteins, although O-mannosylation was intact.

178 , the high alpha selectivity observed with C-mannosylation was reversed to high beta selectivity if t

179 two sites of a rare type of glycosylation (C-mannosylation) were identified at tryptophan residues 43

180 O-Mannosylation, which accounts for up to 30% of the repor

181 of cells deficient for either alpha-DG or O-mannosylation with matriglycans of sufficient length rec