ライフサイエンスコーパス: mannosyltransferase

1 1p outerchain alpha1,6-Man branch-initiating mannosyltransferase.

2 covered the new gene (lpcC) that encodes the mannosyltransferase.

3 that strongly resembles Och 1p, an alpha-1,6-mannosyltransferase.

4 cerevisiae, we have used Och1p, a cis-Golgi mannosyltransferase.

5 o observed with the Saccharomyces cerevisiae mannosyltransferase.

6 at the C-terminal domain is an alpha-(1-->3)-mannosyltransferase.

7 the activity of the C-terminal alpha-(1-->3)-mannosyltransferase.

8 lure to maintain residence of a medial Golgi mannosyltransferase.

9 endogenous inositol phosphorylceramide (IPC) mannosyltransferase.

10 is polymerized by the serotype-specific WbdA mannosyltransferase.

11 yl-P-Man: Man(7)GlcNAc(2)-PP-dolichyl-alpha6-mannosyltransferase.

12 ntial for substrate recognition by the first mannosyltransferase.

13 l for the inositol acyltransferase and first mannosyltransferase.

14 displays distant similarity to yeast protein mannosyltransferases.

15 to dolichol phosphate-D-mannose:protein O-D-mannosyltransferases.

16 (protein O-mannosyltransferase) family of O-mannosyltransferases.

17 motif present in the cytosolic tails of some mannosyltransferases.

18 s of the respective Saccharomyces cerevisiae mannosyltransferases.

19 pha-DG, and it is the substrate of cytosolic mannosyltransferases.

20 by moderate overexpression of several Golgi mannosyltransferases.

21 inhibit many Golgi-located, Mn(2+)-dependent mannosyltransferases.

22 Genetic defects in protein O-mannosyltransferase 1 (POMT1) and POMT2 underlie severe

23 Biallelic mutations in Protein O-mannosyltransferase 1 (POMT1) are among the most common

24 of glycosyltransferases, including protein O-mannosyltransferase 1, beta3-N-acetylglucosaminyltransfe

25 ction, and was termed CMT1, for cryptococcal mannosyltransferase 1.

26 complex of mutually indispensable protein O-mannosyltransferases 1 and 2 (POMT1 and 2).

27 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

28 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

29 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

30 n the parasite Toxoplasma gondii abolished C-mannosyltransferase activity and reduced levels of the m

31 that have both been shown to have alpha-1,6-mannosyltransferase activity in vitro.

32 ha1,6-mannosyltransferase but lacks alpha1,2-mannosyltransferase activity in vivo.

33 evel and strongly suggests that absence of C-mannosyltransferase activity leads to an insufficient le

34 ysis, we demonstrated that the alpha-(1-->2)-mannosyltransferase activity of the N-terminal domain is

35 The loss of C-mannosyltransferase activity was associated with weakene

36 minal domain of WbdA possesses alpha-(1-->2)-mannosyltransferase activity, and we demonstrate in this

37 Both protein complexes have alpha-1, 6-mannosyltransferase activity, forming a series of poly-m

38 domain of WbdA(O9a) possesses alpha-(1-->2)-mannosyltransferase activity.

39 lymphoid-myeloid lineages by suppressing the mannosyltransferase alg2 and sialyltransferase st3gal2,

40 monstrated that Ktr3p, a cis-Golgi-localized mannosyltransferase, also relies on Erv26p for efficient

41 y1 displayed a reduced half-life of the Och1 mannosyltransferase, an established cargo of intra-Golgi

42 ed in the cis-Golgi marker enzymes alpha 1,6 mannosyltransferase and GDPase.

43 uced amino acid sequence shows similarity to mannosyltransferases and other glycosyltransferases.

44 he cytosolic domains of cis and medial Golgi mannosyltransferases and that loss of this interaction c

45 a bifunctional alpha-(1-->2)-, alpha-(1-->3)-mannosyltransferase, and its counterpart in serotype O8

46 e, inositol acyltransferase, all four of the mannosyltransferases, and the ethanolamine phosphate tra

47 nd pattern of conservation in the O9a and O8 mannosyltransferases are not consistent with the existin

48 oss-of-function alleles of ALG12, encoding a mannosyltransferase, as suppressors of a temperature-sen

49 dD-interaction site remained, the N-terminal mannosyltransferase became an unrestricted polymerase, c

50 clustered genes of C. glabrata encoding beta-mannosyltransferases, BMT2-BMT6, were deleted simultaneo

51 ly Golgi compartment that houses an alpha1,6-mannosyltransferase but lacks alpha1,2-mannosyltransfera

52 hat the inhibitors bind to the first alpha-D-mannosyltransferase by means of charge interactions with

53 MNN2 is an alpha-1, 2-mannosyltransferase catalyzing the addition of the first

54 is characterized by deficiency of alpha-1,3-mannosyltransferase caused by pathogenic variants in the

55 The tryptophan C-mannosyltransferase (CMT) enzymes that install the modif

56 ansport was blocked in vivo, subunits of the mannosyltransferase complex accumulated in the vacuole.

57 ervations indicate that the Mnn9p-containing mannosyltransferase complexes cycle back and forth betwe

58 Interestingly, the C-mannosyltransferase-deficient Deltadpy19 parasites were

59 ion of the C. glabrata Anp1, Mnn2, and Mnn11 mannosyltransferases directly affects the structure of t

60 urface-exposed alpha-helix in the C-terminal mannosyltransferase domain of WbdA as the site of intera

61 etermined the role of the dolichol phosphate mannosyltransferase (DPM) complex, a central regulator f

62 mmary, our results indicate that T. gondii C-mannosyltransferase DPY19 is not essential for parasite

63 A single C-mannosyltransferase (dumpy-19, DPY-19), modifying the fi

64 erminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene.

65 homologous family of four putative protein O-mannosyltransferases encoded by the TMTC1-4 genes, which

66 osition -270 from the start codon of PIGM, a mannosyltransferase-encoding gene, disrupts binding of t

67 aromyces cerevisiae mutants lacking multiple mannosyltransferase-encoding genes are hypersensitive to

68 telium discoideum which encodes the beta-1,4-mannosyltransferase enzyme that catalyzes the reaction:

69 synthesized as a polyprenol-linked glycan by mannosyltransferase enzymes located at the cytoplasmic m

70 etylase, inositol acyltransferase, and first mannosyltransferase enzymes.

71 athway to the well-described POMT (protein O-mannosyltransferase) family of O-mannosyltransferases.

72 A second mannosyltransferase from C. neoformans membranes adds ma

73 ylation by deleting the initiating alpha-1,6-mannosyltransferase gene from P. pastoris, several combi

74 knowledge, this study is the first to link a mannosyltransferase gene to osteochondrogenesis.

75 g D-mannosamine (ManN) as a tool to identify mannosyltransferase genes involved in LAM synthesis.

76 to mammalian organisms, Drosophila has two O-mannosyltransferase genes, rotated abdomen (rt) and DmPO

77 Our data suggest that the two Drosophila O-mannosyltransferase genes, rt and tw, have nonredundant

78 In vertebrates, a new C-mannosyltransferase has apparently evolved to increase g

79 lcN-(2-O-alkyl)PI weakly inhibited the first mannosyltransferase in a competitive manner.

80 aromyces cerevisiae, the roles of two of the mannosyltransferases in the pathway, Alg2 and Alg11, hav

81 ence that LpqW regulates the activity of key mannosyltransferases in the periplasmic leaflet of the c

82 The family of mammalian O-mannosyltransferases includes two enzymes, POMT1 and POM

83 Thus, MT1671 is the mannosyltransferase involved in deposition of the first

84 the mptA gene, encoding a membrane alpha1-6-mannosyltransferase involved in extending the alpha1-6-m

85 a family of conserved orthologous protein O-mannosyltransferases is believed to initiate this import

86 nservation between yeast and human protein O-mannosyltransferases is uncharacterized.

87 DID), features that are common to eukaryotic mannosyltransferases (ManTs) of the GT-C superfamily tha

88 iple membrane-associated, substrate-specific mannosyltransferases (ManTs) responsible for the sequent

89 ed to characterize the GDP-mannose-dependent mannosyltransferase MgtA from C. glutamicum that extends

90 The yeast alpha-1,3-mannosyltransferase (Mnn1p) is localized to the Golgi by

91 ogether, these studies have identified a new mannosyltransferase, MptA, and they shed further light o

92 acid substitution within the ppMan-dependent mannosyltransferase MptB could bypass the need for LpqW.

93 Endoplasmic reticulum mannosyltransferases (MTases) are expressed in E. coli a

94 ional cbk1 mutants mislocalize the cis-Golgi mannosyltransferase Och1, are hypersensitive to the amin

95 Two Golgi mannosyltransferases, Och1p and Mnn1p, were mislocalized

96 The natural substrate for the first alpha-D-mannosyltransferase of glycosylphosphatidylinositol bios

97 d with GlcNalpha-PI(C8), confirming that the mannosyltransferase of trypanosomes is divergent from th

98 been determined recently, the structures of mannosyltransferases of the PMT family, which are an int

99 een synthesis and degradation by a family of mannosyltransferase/phosphorylases (MTPs) newly discover

100 Here, we identify a class of dual-activity mannosyltransferase/phosphorylases (MTPs) that catalyze

101 nd domain motions displayed by the essential mannosyltransferase PimA from mycobacteria.

102 ase reactions, and a homology model with the mannosyltransferase PimA, from Mycobacteria smegmatis ,

103 e show for the first time that although both mannosyltransferases PimA and PimB' (MSMEG_4253) recogni

104 The mannosyltransferase PimE catalyzes the transfer of the f

105 Protein O-mannosyltransferases (PMTs) add mannose to serine/threon

106 In analogy, it was assumed that protein O-mannosyltransferases (PMTs) also act at the translocon,

107 Protein O-mannosyltransferases (PMTs) are conserved endoplasmic re

108 Protein O-mannosyltransferases (PMTs) represent a conserved family

109 synthesis, including the two human protein O-mannosyltransferases, POMT1 and POMT2, underlie a subgro

110 dystrophies.SIGNIFICANCE STATEMENT Protein O-mannosyltransferases (POMTs) are evolutionarily conserve

111 Mutations in protein O-mannosyltransferases (POMTs) result in severe brain defe

112 from dolichyl phosphoryl mannose:polypeptide mannosyltransferase (protein mannosyl transferase; PMT),

113 Golgi-bound mannosyltransferases require Mn(2+) as an essential cofa

114 that HOC1 encodes a Golgi-localized putative mannosyltransferase required for the proper construction

115 Thus, MSMEG4245 is apparently a key mannosyltransferase, required for the proper elongation

116 steady-state Golgi localization of multiple mannosyltransferases requires recognition of their cytos

117 nked glycosylation 2 (Alg2), which encodes a mannosyltransferase residing on the endoplasmic reticulu

118 This is in agreement with the mannosyltransferase role predicted for WadC and the lack

119 Previously mutations in a putative protein O-mannosyltransferase (SCO3154, Pmt) and a polyprenol phos

120 Our data indicate that hSmp3p functions as a mannosyltransferase that adds a fourth mannose to certai

121 MNN1 is an alpha-1,3-mannosyltransferase that adds the terminal mannose to th

122 ALG2 is an alpha-1,3-mannosyltransferase that also catalyses early steps in t

123 an PIG-M, an endoplasmic reticulum-localized mannosyltransferase that is required for synthesis of th

124 TMEM260 gene encodes an ER-located protein O-mannosyltransferase that selectively glycosylates IPT do

125 A mannosyltransferase that uses GDP-mannose and the conser

126 doplasmic reticulum by a family of protein O-mannosyltransferases that are conserved from yeast (PMTs

127 MNN10 and MNN11 encode mannosyltransferases that are part of the N-glycosylatio

128 ormational transitions are important for the mannosyltransferase to interact with the donor and accep

129 MSMEG_4241 mutant that lacks the alpha-(1,6)-mannosyltransferase used late in LM elongation, we showe

130 s of this synthetic lethality included three mannosyltransferases, VAN1, KTR4, and HOC1.

131 mbrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial

132 ases, WbdC and WbdB, and a serotype-specific mannosyltransferase, WbdA.

133 nol pyrophosphoryl-GlcpNAc, by two conserved mannosyltransferases, WbdC and WbdB, and a serotype-spec

134 lly and pharmacologically blocking protein O-mannosyltransferases, we found that this posttranslation

135 accharomyces cerevisiae ScAlg2, an alpha-1,2-mannosyltransferase, which functions in the early stages

136 maintains the Golgi localization of several mannosyltransferases, which is subsequently critical for

137 e, we initiated purification of an alpha-1,3-mannosyltransferase with appropriate specificity for a r

138 A novel mannosyltransferase with specificity appropriate for a r

139 lustrate assembly systems exploiting several mannosyltransferases with flexible active sites, arrange