ライフサイエンスコーパス: membrane protein

1 led enrichment of specific lipids around the membrane protein.

2 the need to purify or label the investigated membrane protein.

3 ng effects on the phenotype of this integral membrane protein.

4 ble proteins, its use remains negligible for membrane proteins.

5 idiscs can stabilize such a large variety of membrane proteins.

6 ransport for directed trafficking of certain membrane proteins.

7 e correct folding and expression of integral membrane proteins.

8 ology by targeting these receptor-associated membrane proteins.

9 key element of the function of many integral membrane proteins.

10 late to studying the effects of mutations in membrane proteins.

11 abels to probe the structure and dynamics of membrane proteins.

12 i trafficking of different classes of apical membrane proteins.

13 and are broadly used for characterization of membrane proteins.

14 lvents and functional cofactors for integral membrane proteins.

15 much less is known about quality control of membrane proteins.

16 o understand the biogenesis of this class of membrane proteins.

17 the complexes of sugars that decorate these membrane proteins.

18 ation with synaptic vesicles, especially for membrane proteins.

19 required for quality control of a subset of membrane proteins.

20 ing studies and has previously been used for membrane proteins.

21 d mutants with defective apical targeting of membrane proteins.

22 ome through the clearance of mislocalized ER membrane proteins.

23 a fundamental step in the folding of helical membrane proteins.

24 noncovalent binding of ligands and lipids to membrane proteins.

25 n about the mechanisms required for assembly membrane proteins.

26 gnals for both the insertion and assembly of membrane proteins.

27 provides a realistic pH-dependent model for membrane proteins.

28 mote raft partitioning for multispan helical membrane proteins.

29 educed biogenesis of numerous multi-spanning membrane proteins.

30 afficking is mediated by lysosome-associated membrane protein 1 (LAMP-1)-positive vesicles based on s

31 the endogenous locus of lysosome-associated membrane protein 1 (LAMP1).

32 inase (JNK) signaling, induced by the latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV).

33 ked whether the PRPH2 binding partner rod OS membrane protein 1 (ROM1) could serve as a phenotypic mo

34 the PRPH2 binding partner, rod outer segment membrane protein 1 (ROM1), and rhodopsin were mislocaliz

35 bility to oligomerize with rod outer segment membrane protein 1 (Rom1), but retained the ability to f

36 mutations in CLN3, which encodes a lysosomal membrane protein(1-3).

37 Vesicle associated membrane protein 2 (VAMP2/synaptobrevin2), a core SNARE

38 e key viral factor in this process is latent membrane protein 2A (LMP2A), which has been described as

39 mRNA levels for lysosomal-associated membrane protein 3 (LAMP3), a gene that is highly up-reg

40 receptor (SNARE) molecule vesicle-associated membrane protein 4 (VAMP4) as a key component of the mac

41 ogous architectures of related inner nuclear membrane proteins(7,8), suggest that phase separation ma

42 her overexpression of the vesicle-associated membrane protein 8 (VAMP8), an R-SNARE found on late end

43 ytophilum surface protein (Asp14), and outer membrane protein A (OmpA) are essential for optimal bact

44 to confirm the spontaneous folding of outer membrane protein A (OmpA) into preformed NDs.

45 junction (TJ) formed by specialized adhesive membrane proteins, adaptor proteins, and the actin cytos

46 to understand the underlying mechanism(s) of membrane protein aggregation.

47 signment method, for example, to investigate membrane protein allostery and drug binding in a more na

48 The fortuitously discovered antiaging membrane protein alphaKlotho (Klotho) is highly expresse

49 synthesized as an endoplasmic reticulum (ER) membrane protein and when cellular proteasome activity i

50 Detergents enable the purification of membrane proteins and are indispensable reagents in stru

51 a phylogroups for putative beta-barrel outer membrane proteins and considered their potential as vacc

52 d random heteropolymers (RHPs)(14) can mimic membrane proteins and exhibit selective proton transport

53 structural and functional investigations of membrane proteins and for preparation of suitable platfo

54 tations or truncations, and is challenged by membrane proteins and large multicomponent complexes.

55 Membrane proteins and lipids coevolved to yield unique c

56 ial pathogen Vibrio cholerae jettisons outer membrane proteins and lipids in vesicles as it enters th

57 bly to minimize misfolding of multi-spanning membrane proteins and maintain cellular protein homeosta

58 ad map for studying the interactions between membrane proteins and synthetic membranes, which will be

59 e insertion for numerous eukaryotic integral membrane proteins and tail-anchored proteins.

60 ment depends upon interactions between viral membrane proteins and tegument proteins that encrust cap

61 significant advance for the investigation of membrane proteins and their interactions with lipids.

62 rs (XFELs) allows structure determination of membrane proteins and time-resolved crystallography.

63 tool can be applied to diverse alpha-helical membrane proteins, and may aid in the development of oth

64 organelles contain around 70 hydrolases, 200 membrane proteins, and numerous accessory proteins assoc

65 mple sequence motifs can rigidify elementary membrane proteins, and that orthogonal artificial membra

66 naive scFv libraries to be selected against membrane protein antigens in a Chinese hamster ovary cel

67 Our cumulative data indicate that these RND membrane proteins are able to utilize different oligomer

68 ectrophysiological measurements suggest that membrane proteins are affected as well, particularly vol

69 resolution structures of large complexes and membrane proteins are determined regularly.

70 Integral membrane proteins are exposed to a complex and dynamic l

71 the stability, organization, and function of membrane proteins are influenced by certain lipids and s

72 In eukaryotes, the majority of membrane proteins are inserted, modified and folded at t

73 Type II tail-anchored (TA) membrane proteins are involved in diverse cellular proce

74 After ubiquitination, vacuole membrane proteins are sorted into the lumen for degradat

75 the past several decades has determined how membrane proteins are targeted to the ER and how individ

76 culum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the

77 ss spectrometry to identify ligands bound to membrane protein assemblies.

78 polymers that allow isolation of endogenous membrane:protein assemblies in native nanodiscs without

79 agosome is seeded by small vesicles carrying membrane protein autophagy-related 9 (ATG9), the functio

80 We applied this protocol to the model membrane protein bacteriorhodopsin (bR).

81 lso works for the largest currently assigned membrane protein, BamA with 398 residues.

82 lar interactions with the coherently ordered membrane proteins become visible in real time, while non

83 Bacterial membrane proteins belonging to the resistance-nodulation

84 e populated with a host of beta-barrel outer-membrane proteins (betaOMPs).

85 successful for quantification of peripheral membrane protein binding to the PM in living cells.

86 Membrane protein biogenesis faces the challenge of chape

87 Membrane protein biogenesis in the endoplasmic reticulum

88 Consistent with a role in multi-pass membrane protein biogenesis, cells lacking different acc

89 otein levels and a broader role in polytopic membrane protein biogenesis.

90 these proteins is controlled by two integral membrane proteins, BlaR1 and MecR1, which both have an e

91 PE is predicted to be an anchored membrane protein, but its topological organization is un

92 We then apply the strategy to an integral membrane protein by comparing the shapes of a prokaryoti

93 s provide a suitable platform to investigate membrane proteins by a broad range of surface-sensitive

94 s that control the abundance and function of membrane proteins by cleaving their substrate's extracel

95 or charge reduction of detergent-solubilized membrane proteins by native MS.

96 uptake is mediated by an inner mitochondrial membrane protein called the mitochondrial calcium unipor

97 Skin-specific basement membrane proteins called laminins play important roles i

98 ane proteins, and that orthogonal artificial membrane proteins can influence the cofactor repertoire

99 to branch out in many directions, including membrane protein characterization, endocytosis, secretio

100 and cryoEM data of several examples of BRIL-membrane protein chimera highlight the effectiveness of

101 associated with accumulation of the basement membrane protein, collagen IV, in LVV-forming endothelia

102 Structural homology with YidC and the ER membrane protein complex (EMC) implicates an evolutionar

103 The endoplasmic reticulum (ER) membrane protein complex (EMC) was identified over a dec

104 gest a model in which spatial segregation of membrane protein complex assembly and quality control im

105 ver a link between INM proteome identity and membrane protein complex assembly in the remaining ER.

106 e, we used a transposon screen to identify a membrane protein complex that spatially regulates S. aur

107 Ejecting the membrane protein complex with bound lipids in the mass s

108 e charge-reducing molecules do not adduct to membrane protein complexes and are also compatible with

109 ometry (MS) provides the capacity to monitor membrane protein complexes and noncovalent binding of li

110 f even larger, more complex systems, namely, membrane protein complexes and their interactions with l

111 lications of mass spectrometry (MS) to study membrane protein complexes are yielding valuable insight

112 These membrane protein complexes possess numerous subunit isof

113 le approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute

114 Alpha-helical integral membrane proteins contain conserved sequence motifs that

115 Here, we demonstrate that RocA is a membrane protein containing seven transmembrane helices

116 holipid bilayers with two different types of membrane proteins, CorA and tissue factor (TF).

117 al effects, as the function of many embedded membrane proteins depends on phospholipid bilayer biophy

118 lthough soluble protein design has advanced, membrane protein design remains challenging because of d

119 d there have been recent advances in de novo membrane protein design(7,8) and in redesigning naturall

120 tion in Pantoea sp. YR343 results in altered membrane protein distribution and abundance.

121 a model and show that a conserved cis-Golgi membrane protein eas-1/GOLT1B negatively regulates glial

122 d secretion of two distinct families of T6SS membrane protein effectors.

123 ve studies of larger complex systems such as membrane proteins embedded in nanodiscs.

124 rategy for top-down proteomics of endogenous membrane proteins enabled by cloud point extraction and

125 GPCRs are the largest family of membrane proteins encoded in the human genome and are th

126 Membrane proteins engage in a variety of contacts with t

127 d these new insights will offer guidance for membrane protein engineering.

128 salts caused marked charge reduction in the membrane protein, Erwinia ligand-gated ion channel (ELIC

129 CRISPR/Cas9-based functional screening of 59 membrane proteins expressed in the gametocytes of Plasmo

130 yet specializes Msp1 for its unique role in membrane protein extraction.

131 nslocase cooperates with the Cdc48 ATPase in membrane protein extraction.

132 largest and most pharmacologically targeted membrane protein family.

133 st rods," which likely represent accumulated membrane proteins following defective exocytosis or recy

134 modular strategy for directing secreted and membrane proteins for lysosomal degradation, with broad

135 elin oligodendrocyte glycoprotein (MOG), the membrane proteins found in the myelin sheath.

136 strates the selection and export of integral membrane proteins from the endosome via retrograde and p

137 peroxins that mediate import of peroxisomal membrane proteins from the ER, hinting at dual localizat

138 The Tat machinery comprises membrane proteins from the TatA and TatC families.

139 we monitored transport of newly synthesized membrane proteins from the TGN to apical membrane in liv

140 Sorting of integral membrane proteins from the trans-Golgi network (TGN) has

141 Trafficking of photoreceptor membrane proteins from their site of synthesis in the in

142 the perturbing effects of drug candidates on membrane protein function, have implications for preclin

143 branes may produce indiscriminate changes in membrane protein function.

144 ipid order, which raises the question of how membrane proteins function in such an environment.

145 atly influence the thermodynamics underlying membrane-protein functions, including ligand binding, al

146 Two HSV membrane proteins, gE/gI and US9, play an essential role

147 insights into the structure and function of membrane proteins have been obtained using detergents; h

148 The internal motions of integral membrane proteins have largely eluded comprehensive expe

149 tions on the natural dynamics of the labeled membrane proteins have not been well studied.

150 st membrane proteome revealed that polytopic membrane proteins have relatively low ribosome abundance

151 M LRS as a model, we demonstrate how the LRS membrane protein HdrM inhibits its cognate transcription

152 e for G-protein-coupled receptors, which are membrane proteins implicated in cellular signal transduc

153 tant low-resolution information for integral membrane proteins (IMPs), challenging targets for struct

154 ic peptide (ANP, BNP) is amidated, the major membrane protein in atrial granules is peptidylglycine a

155 er nanodiscs are an attractive tool to study membrane proteins in a detergent-free lipid-bilayer envi

156 is crucial for the structure and function of membrane proteins in all cells.

157 tein-coupled receptors (GPCRs) are important membrane proteins in higher eukaryotes that carry out a

158 evertheless, the successful incorporation of membrane proteins in lipid bilayers with sufficiently hi

159 laques, suggesting a major role for basement-membrane proteins in maintaining plaque stability.

160 l density and the abundance of mitochondrial membrane proteins in skeletal muscle increased during la

161 as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018).

162 native to detergents as a means to stabilize membrane proteins in solution for structural and functio

163 alues broadly similar to values reported for membrane proteins in supported lipid bilayers.

164 A large number of newly synthesized membrane proteins in the endoplasmic reticulum (ER) are

165 ns to antagonize SERINC5.IMPORTANCE Cellular membrane proteins in the SERINC family, especially SERIN

166 e required for the formation of complexes of membrane proteins including cell-wall synthetic proteins

167 hat regulate numerous cellular processes and membrane proteins, including ENaC.

168 show that ES24 impairs protein secretion and membrane protein insertion in Escherichia coli via the h

169 s anionic phospholipids through an extensive membrane:protein interface.

170 ompared the extraction and reconstitution of membrane proteins into lipid nanodiscs by a series of zS

171 rane helix (TMH) of many multi-pass integral membrane proteins into the ER membrane, and it is also r

172 s (GPCRs) comprise a large class of integral membrane proteins involved in the regulation of a broad

173 ling pathways activated downstream of the ER membrane proteins IRE1, ATF6, and PERK.

174 Down-regulation of vacuole membrane proteins is initiated by ubiquitination, which

175 A particular challenge, especially for membrane proteins, is preserving noncovalent interaction

176 The mycobacterial membrane protein large (MmpL) proteins transport cell en

177 grity, a process involving the inner nuclear membrane protein LEM2 recruiting CHMP7/Cmp7 and then ESC

178 hanges in the conformational ensemble of the membrane protein LeuT.

179 tudies are challenging regarding multidomain membrane proteins like CusS and also lack the physiologi

180 reducing agents when dissociated from native membrane protein-lipid complexes in the gas phase and pr

181 s extremely challenging to identify specific membrane protein-lipid interactions and their relative s

182 oupling, and provide a paradigm for studying membrane protein-lipid interactions for class B GPCRs.

183 The integral membrane protein Lit (lipoprotein intramolecular transac

184 ogenic EBV nuclear antigen (EBNA) and latent membrane proteins (LMPs), is expressed in newly infected

185 Basement-membrane protein loss is a prominent feature of ruptured

186 Lymphoid-restricted membrane protein (LRMP, Jaw1) and inositol trisphosphate

187 annosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for p

188 (SLC16) family represents a diverse group of membrane proteins mediating the transport of monocarboxy

189 cells, we identified the mitochondrial outer membrane protein mitochondrial carrier homolog 2 (MTCH2)

190 ent ubiquitination of the outer mitochondria membrane protein mitofusin1.

191 This work highlights universal membrane protein motifs, including lipid-protein interac

192 Membrane proteins (MPs) used to be the most difficult ta

193 we used native or nondenaturing MS to ionize membrane protein nanodiscs with heterogeneous lipids.

194 dynein-binding domains in the outer nuclear membrane protein nesprin-2G, which polarizes the inner n

195 glycans and ligand binding on the influenza membrane protein neuraminidase.

196 nsists of a single exon, encoding a putative membrane protein of 127 amino acids.

197 Myelin protein P2 is a peripheral membrane protein of the fatty acid-binding protein famil

198 s compared with the mobility of Sec61beta, a membrane protein of the SR unrelated to the EC coupling

199 The prototype membrane protein of this family, AceI (Acinetobacter chl

200 vivo interactors of AtGET1 and identified a membrane protein of unknown function with low sequence h

201 ain obstacle for extending this technique to membrane proteins of arbitrary topology has remained in

202 (CC) domain of Grp1 engaging two peripheral membrane proteins of the recycling endosome.

203 The latter contains integral membrane proteins of three sizes, collectively known as

204 ations (PTMs) of Hb and red blood cell (RBC) membrane proteins of transgenic SCD mice.

205 The charge states produced by native MS of membrane proteins often result in gas-phase protein unfo

206 Membrane protein oligomerization mediates a wide range o

207 mic chaperone SurA plays a key role in outer membrane protein (OMP) biogenesis.

208 Bacterial outer membrane proteins (OMPs) contain a unique "beta barrel"

209 Integral beta-barrel outer membrane proteins (OMPs) function to establish and maint

210 e folding and insertion of beta-barrel outer membrane proteins (OMPs) to the outer membrane are media

211 asibility of the approach on the 148-residue membrane protein OmpX.

212 id bilayer with a relatively high density of membrane proteins on the support surface.

213 ntify a panel of three novel binders to this membrane protein, one with a dissociation constant (K(D)

214 are known as crystallization chaperones for membrane proteins or as simple alternatives to conventio

215 conformation-specific binders against labile membrane proteins or protein complexes and allows select

216 ct de novo the structures of large proteins, membrane proteins, or proteins of complex topologies.

217 n near the native amino acid distribution in membrane proteins, overcoming a critical flaw in previou

218 Mitochondrial membrane protein p32 can block mtDNA synthesis by restri

219 nvolved in transportation and degradation of membrane, proteins, pathogens, and organelles.

220 Here we characterize the inner membrane protein PbgA and report that its depletion atte

221 ecruited to the organelle by the peroxisomal membrane protein Pex3.

222 The cardiac membrane protein phospholamban (PLN) is targeted by prot

223 strongly suggest that extensive erythrocyte membrane protein phosphorylation and ubiquitination are

224 lity of outer hair cells, underpinned by the membrane protein prestin, to expand the frequency range.

225 he ageing OHCs retained a normal basolateral membrane protein profile, they showed a reduction in the

226 We combined steered molecular dynamics and membrane protein-protein docking experiments to achieve

227 nchored AAA+ protease, plays a vital role in membrane protein quality control.

228 indings reveal 45 integral and 51 peripheral membrane proteins re-routed by golgin-97, evidence for a

229 Diminished frequencies of membrane protein-reactive IFN-gamma+ T cells were partic

230 In both contexts, a membrane protein related to bacterial RND transporters r

231 In meso crystallization of membrane proteins relies on the use of lipids capable of

232 ological systems, the analysis of endogenous membrane proteins remains challenging due to their low s

233 interactions in bilayers and understand how membrane proteins remodel their surrounding lipid enviro

234 Small membrane proteins represent a largely unexplored yet abu

235 d formation of supported lipid bilayers with membrane proteins represents an attractive strategy for

236 of detergents for individual applications in membrane protein research.

237 ptors (GPCRs) are a large family of integral membrane proteins responsible for cellular signal transd

238 of a C. trachomatis recombinant major outer membrane protein (rMOMP) vaccine to elicit cross-serogro

239 metry (XFMS) on large protein assemblies and membrane protein samples requires high flux density to o

240 activation requires the cholesterol-sensing membrane protein Scap.

241 r localizations of SpmX, a low-copy integral membrane protein sequestered by PopZ as part of a signal

242 The human integral membrane protein SERINC5 potently restricts HIV-1 infect

243 ggest that most, if not all, prokaryotic LRS membrane proteins serve as inhibitors of their cognate t

244 geting structural proteins, most importantly membrane protein, should be feasible for the prevention

245 nt developed for crystallography to increase membrane protein stability.

246 Our method advances high-resolution membrane protein structure prediction and design toward

247 ng an example of how ordered membranes alter membrane protein structure.

248 ds provide modest thermodynamic stability to membrane protein structures and that many amides are una

249 vs. cavities for function, are reconciled in membrane protein structures.

250 both quaternary- and internal-symmetries in membrane protein structures.

251 Motivated by the fact that in vitro membrane protein studies often require additives such as

252 The activity of SERCA is regulated by small membrane protein subunits, the most well-known being pho

253 a, as well as reduced abundance of lysosomal membrane proteins such as LAMP1.

254 e of syntaxin 3 (STX3), in trafficking of OS membrane proteins such as peripherin 2 (PRPH2) and rhodo

255 esprin-2G, which polarizes the inner nuclear membrane protein SUN1.

256 In membrane proteins, symmetry and pseudosymmetry often hav

257 whereas inactivating cell division or outer membrane protein synthesis blocked it the slowest.

258 e, we identify the C. difficile heme-sensing membrane protein system (HsmRA) and show that this opero

259 ability data, and in vitro tests using three membrane protein targets with 7, 11 and 16 transmembrane

260 The Na(+)/I(-) symporter (NIS), the plasma membrane protein that actively transports I(-) (stoichio

261 integral membrane M2 protein is a 97-residue membrane protein that assembles as a tetramer to conduct

262 fied S. cerevisiae Mps2 as the outer nuclear membrane protein that connects the LINC complex with the

263 age-gated Hv1 proton channel is a ubiquitous membrane protein that has roles in a variety of cellular

264 en of the prostate 1 (STEAP1) is an integral membrane protein that is highly up-regulated on the cell

265 ntercellular adhesion molecule-1 (ICAM-1), a membrane protein that mediates cell-to-cell adhesion and

266 BAX inhibitor-1 motif-containing 6 (TMBIM6) membrane protein that plays a key role in the control of

267 BTNL2 is a type-1 membrane protein that provides inhibitory signal to T ce

268 bD functional homolog CcdA is a six-TM-helix membrane protein that provides reducing equivalents for

269 SERINC5 is a multipass intrinsic membrane protein that suppresses HIV-1 infectivity when

270 ly 25% of eukaryotic genes code for integral membrane proteins that are assembled at the endoplasmic

271 ptor tyrosine kinases (RTKs) are single-pass membrane proteins that control vital cell processes such

272 re-emptive pathway that reduces synthesis of membrane proteins that have failed to properly assemble

273 G-protein-coupled receptors (GPCRs) are membrane proteins that modulate physiology across human

274 t system consists of a network of plasma and membrane proteins that modulate tissue homeostasis and c

275 In oriented-sample (OS) solid-state NMR of membrane proteins, the angular-dependent dipolar couplin

276 nterference demonstrate that one of five AdV membrane proteins, the E3-19K glycoprotein specifically

277 icum and that acetylation is mediated by the membrane protein TmaT.

278 events ATG16L1 interaction with the integral membrane protein TMEM59 and allows the rerouting of Rab6

279 a crystal structure of the full-length LCI1 membrane protein to reveal LCI1 structural characteristi

280 However, the mechanisms targeting membrane proteins to the apical surface are still poorly

281 ons can affect the structure and dynamics of membrane proteins to various extents, especially in syst

282 anism is analogous to that used by the inner membrane protein TonB to dislodge the plug domains of ou

283 e phenotypes through dysfunctional multipass membrane protein topogenesis.

284 ex virus (HSV) heterodimer gE/gI and another membrane protein, US9, which has neuron-specific effects

285 cloud point extraction efficiently enriched membrane proteins using a single extraction, eliminating

286 s as reporters of conformational dynamics of membrane proteins using DEER spectroscopy.

287 cle membrane (R-SNAREs or vesicle-associated membrane proteins [VAMPs]) and the target membrane (Q-SN

288 re is a previously undescribed mutant of the membrane protein VDAC, crystallized in a lipid bicelle m

289 bly factor vacuolar ATPase assembly integral membrane protein (VMA21), whose X-linked mutations lead

290 nsory rhodopsin II and the beta-barrel outer membrane protein W have been investigated in lipid bilay

291 In an effort to reduce the charge of membrane proteins, we examined the utility of alkali met

292 ble as the NPC1 gene product is an insoluble membrane protein, which increases the need for developme

293 dentifying novel antibodies that act against membrane proteins, which could catalyze the discovery of

294 sensing and sequestration of dsRNAs encoding membrane proteins, which promote ER homeostasis by activ

295 model may be operational in other peripheral membrane proteins with an unprecedented impact in drug d

296 ers commonly seek to correlate activities of membrane proteins with attributes of the domains in whic

297 We found that membrane proteins with C-TMD tails shorter than ~60 amin

298 very important feature, the strategy allows membrane proteins with one large extramembrane domain to

299 ery little is known about how multi-spanning membrane proteins with several TMDs are assembled within

300 How the inner membrane protein YejM with its periplasmic domain change