ライフサイエンスコーパス: mercaptoethanol

1 thiol like DTT (dithiothreitol) and 2-ME (2-mercaptoethanol).

2 uantitatively in the presence of excess beta-mercaptoethanol.

3 le in the presence of heating, SDS, and beta-mercaptoethanol.

4 o-phthalaldehyde (OPA) in the presence of 2-mercaptoethanol.

5 bond by removal with dithiothreitol or beta-mercaptoethanol.

6 highly stable and resistant to SDS and beta-mercaptoethanol.

7 also inhibited by DMSO and reversed by beta-mercaptoethanol.

8 d by boiling in the presence of SDS and beta-mercaptoethanol.

9 guanidine HCl, 8 m urea, 2% SDS, or 5% beta-mercaptoethanol.

10 ercuric chloride was readily reversible by 2-mercaptoethanol.

11 was denatured by heat or reduced using beta-mercaptoethanol.

12 ex is dissociated by reducing agents such as mercaptoethanol.

13 se but attenuated the effect of both GSH and mercaptoethanol.

14 ts such as 5 mM dithiothreitol or 10 mM beta-mercaptoethanol.

15 ccurred in the presence of an excess of beta-mercaptoethanol.

16 destabilized by D-ribulose-5-phosphate or 2-mercaptoethanol.

17 ercurial inhibition was reversible with beta-mercaptoethanol.

18 d by DEAE chromatography in 6 M urea/4% beta-mercaptoethanol.

19 e completely suppressed by the addition of 2-mercaptoethanol.

20 tial amino acids, fetal calf serum, and beta-mercaptoethanol.

21 ty of FDH occurred only in the presence of 2-mercaptoethanol.

22 The enzymatic activity was increased by beta-mercaptoethanol.

23 amin, Fe(3+), and sulfide in the presence of mercaptoethanol.

24 was denatured and reduced with urea and beta-mercaptoethanol.

25 292Asp enzyme showed complete preference for mercaptoethanol.

26 d to the 31-kDa band by the reducing agent 2-mercaptoethanol.

27 RNeasy Fibrous Tissue Kit combined with beta-mercaptoethanol.

28 ion by H2O2 or reduction by glutathione or 2-mercaptoethanol.

29 he radical scavengers ascorbic acid and beta-mercaptoethanol.

30 inylation occurred after treatment with beta-mercaptoethanol.

31 is subsequently achieved with an excess of 2-mercaptoethanol.

32 GR active site with thiol reagents such as 2-mercaptoethanol.

33 18:1-NO2 detected by exchange to added beta-mercaptoethanol.

34 rotein was denatured in the presence of beta-mercaptoethanol.

35 nline with o-phthaldialdehyde (OPA) and beta-mercaptoethanol.

36 rongly inhibited by high concentrations of 2-mercaptoethanol.

37 significantly quenched by 450-fold excess 2-mercaptoethanol.

38 tivate GABA-AT, but only in the absence of 2-mercaptoethanol.

39 air, but not in the presence of 100 mM beta-mercaptoethanol.

40 pendent inhibitor, even in the presence of 2-mercaptoethanol.

41 Trapping of 1 by O2 or beta-mercaptoethanol (1 M) does not compete with strand sciss

42 300 microM) and the lipophilic antioxidant 2-mercaptoethanol (10 microM) both protected significantly

43 0 (20 mm), PA (2 mm), Mg(2+) (0.5 mm), and 2-mercaptoethanol (10 mm) at pH 7.5 and 30 degrees C.

44 L(R, S) recovered fully after transfer to 2-mercaptoethanol (10 mM).

45 The sulfhydryl reducing agent mercaptoethanol (10-100 microM) mimicked the action of G

46 Treatment of these sera with 2-mercaptoethanol (2-ME) reduced, but did not eliminate, t

47 rythrocytes included: pronase, trypsin, beta-mercaptoethanol (2-ME), and heating to 90 degrees .

48 an structurally similar monothiols such as 2-mercaptoethanol (2-ME), that cleavage by DTT exhibits sa

49 ation coefficient between IgG anti-LPS and 2-mercaptoethanol (2-ME)-resistant vibriocidal antibodies

50 unit/mL) with heat (95 degrees C, 5 min), 2-mercaptoethanol (2-ME, 0.83 %), and l-cysteine (l-Cys, 5

51 ion by sodium dodecyl sulfate (SDS) and beta-mercaptoethanol (2ME), suggesting the recognition of non

52 A synthetic vitamin K analogue, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or compound

53 hown that a synthetic vitamin K analog, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or compound

54 epidermal growth factor, we found that 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone or Compound

55 Compound 5 (Cpd 5) or 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone, is an inhi

56 robed the effects of a Cdc25 inhibitor, 2-(2-mercaptoethanol)-3-methyl-1,4-naphthoquinone, or Compoun

57 enotype was canceled by the ADSS inhibitor 6-mercaptoethanol (6-MP).

58 Treatment of DENV-2 with beta-mercaptoethanol abolished binding of Fab 1A5, indicating

59 Treatment of protein samples with 2-mercaptoethanol abolished the S-thiolation signals detec

60 of the NCS-chrom C-6 radical, generated by 2-mercaptoethanol activation, to C-5 of the uracil at the

61 resumably due to their purification (i.e., 2-mercaptoethanol adducts and carbamylation) and related t

62 n be released, trapped, and detected as beta-mercaptoethanol adducts by mass spectrometry.

63 n-5-maleic acid, and the L-cysteine and beta-mercaptoethanol adducts of EM in aqueous and hydrophobic

64 e mass measurement was used to identify beta-mercaptoethanol adducts of sulforaphane that had been re

65 such as tris(2-carboxyethyl)phosphine and 2-mercaptoethanol also abrogate NS5A-NS5A interactions, im

66 ent of alpha1PI with the reducing agent beta-mercaptoethanol also inhibited binding of trypsin to alp

67 hiol-reducing agents dithiothreitol and beta-mercaptoethanol also inhibited high affinity [3H]ryanodi

68 vatized on-line with o-phthaldialdehyde/beta-mercaptoethanol and automatically transferred to a separ

69 7,8-dihydrobenzo[a]pyrene was trapped with 2-mercaptoethanol and characterized as a thioether conjuga

70 100-fold higher than that observed for beta-mercaptoethanol and cysteine, suggesting that thioredoxi

71 vatized on-line with o-phthaldialdehyde/beta-mercaptoethanol and detected by LIF using the 354 nm lin

72 gnature-events were abolished by 100 mM beta-mercaptoethanol and did not occur in a cysteineless gain

73 tivity was demonstrated in the presence of 2-mercaptoethanol and dithiothreitol.

74 Data analysis shows that beta-mercaptoethanol and ethanol both interact or bind prefer

75 in 2% sodium ascorbate containing 10mM beta-mercaptoethanol and heated to release the folates.

76 tment with 2% sodium dodecyl sulfate-1% beta-mercaptoethanol and heating to 100 degrees C for 5 min.

77 utathione (GSH), N-acetyl-cysteine, and beta-mercaptoethanol and identified the adducts.

78 Two thiols, 2-mercaptoethanol and mercapto-2-propanol, were poorer sub

79 activation was blocked by the antioxidants 2-mercaptoethanol and N-acetyl-L-cysteine, suggesting that

80 derivatization by o-phthalaldehyde and beta-mercaptoethanol and optically gated capillary electropho

81 However, Cu(2+) and Mn(2+) ions, Mercaptoethanol and Triton X-100 reduce the activity of

82 de heterodimer was sensitive to reduction by mercaptoethanol and was enhanced by the thioredoxin-redu

83 he presence of a sacrificial electron donor (mercaptoethanol) and continuous irradiation, the resulti

84 ne, homocysteine, cysteinylglycine, and beta-mercaptoethanol) and human serum albumin.

85 es, a reducing agent (dithiothreitol or beta-mercaptoethanol), and Mg2+ and was resistant to inhibito

86 Tris (pH 7.5), 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4 degrees C].

87 s-HCl, pH 7.5, 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4.0 degrees C].

88 as observed with cysteine, dithiothreitol, 2-mercaptoethanol, and 3-mercaptopropionate.

89 lactone, trifluoromethylarachidonoyl ketone, mercaptoethanol, and ATP, but was inhibited by MJ33 and

90 Indeed, both quinones form adducts with mercaptoethanol, and beta-lapachone is 10-fold more reac

91 The CoM analogues 2-mercaptopropionate, 2-mercaptoethanol, and cysteine substituted poorly for CoM

92 Glutathione, mercaptoethanol, and dihydrolipoate support the hydroxyl

93 thiol reagents, including glutathione, beta-mercaptoethanol, and dithiothreitol.

94 tivation of spinach PRK by dithiothreitol, 2-mercaptoethanol, and glutathione was examined.

95 not with sodium dodecyl sulfate (SDS) and 2-mercaptoethanol, and migrated as polydisperse, high-mole

96 mall molecules (chloroform, dichloromethane, mercaptoethanol, and nitric oxide), we present evidence

97 the solvent effects of a co-substrate, beta-mercaptoethanol, and of a model nonsubstrate, ethanol, o

98 h proteinase K, boiled in detergent and beta-mercaptoethanol, and subjected to sucrose density gradie

99 d with three different reductants (DTT, beta-mercaptoethanol, and TCEP).

100 Reducing agents, such as DTT, 2-mercaptoethanol, and thioredoxin, were able to activate

101 other reagents such as polyethylene glycol, mercaptoethanol, and urea were also tested in the assay.

102 thiols methyl-3-mercaptopropionate and beta-mercaptoethanol are shown to add exclusively and quantit

103 Dioxygen and beta-mercaptoethanol are unable to compete with loss of uraci

104 amino acid derivatives, generated using beta-mercaptoethanol as a nucleophile, was characterized unde

105 hydryl compounds such as l-cysteine and beta-mercaptoethanol as cosubstrates.

106 vity toward thiols like dithiothreitol and 2-mercaptoethanol as well as reagents: mixed disulfides (m

107 2-Mercaptoethanol assists in the conversion of monomers to

108 n with sodium-dodecyl-sulfate (SDS) and beta-mercaptoethanol at 50-60 degrees C for >=1 h), followed

109 e reducing agents-dithiothreitol (DTT), beta-mercaptoethanol (beta-MCE), and tris(2-carboxyethyl)phos

110 These mutants produce a beta-mercaptoethanol (beta-ME) adduct of the 2'-deoxyuridine-

111 de linkage by 1,4-dithiothreitol (DTT), beta-mercaptoethanol (beta-ME) or cysteine at low temperature

112 Transient treatment with the reductant beta-mercaptoethanol (beta-ME) was able to partially rescue t

113 Urea, ethanol, and beta mercaptoethanol (beta-ME) were used to evaluate the effe

114 fferent photostabilizing agents, namely beta-mercaptoethanol (beta-ME), Trolox (TX), n-propyl gallate

115 y can be restored by the treatment with beta-mercaptoethanol (beta-ME).

116 The reducing agent beta-mercaptoethanol (betaME) reversibly decreased the adhesi

117 ue-fluorescent state by incubation with beta-mercaptoethanol (betaME).

118 rcular dichroism spectra of the H93G Mb beta-mercaptoethanol (BME) thiolate adduct reveal a high-spin

119 ed kinetic studies of the reaction with beta-mercaptoethanol (BME) yielded the second-order rate cons

120 In the presence of 30 mM beta-mercaptoethanol (BME), the k(cat) increased to 176 min(-

121 Our results show that ethanol and beta-mercaptoethanol both alter the equilibrium distribution

122 Incubation of Dex3(1 kD)-Cyt c with mercaptoethanol caused significant loss in the tertiary

123 be co-localized on immunoblots of yeast beta-mercaptoethanol cell wall extracts and cytosolic fractio

124 The free radical scavenger 2-mercaptoethanol completely suppressed ROS generation by

125 roteins extracted from coats with urea and 2-mercaptoethanol could, after removal of urea by gel filt

126 ihydrobiopterin and other thiols (E.G., beta-mercaptoethanol, cysteine, and glutathione, with less ef

127 Higher concentrations of beta-mercaptoethanol decrease the concentration of the quinon

128 We encountered beta-mercaptoethanol-dependent artifact signals in western bl

129 /mass spectrometry of the o-phthalaldehyde/2-mercaptoethanol derivatives and identified as citrulline

130 The procedure was used in parallel with mercaptoethanol derivatization and LC-MS(2) analysis to

131 Treatment with beta-mercaptoethanol did not impact the apparent molecular we

132 Three types of beta-mercaptoethanol-dissociable complexes were visualized wi

133 not observed with several more polar thiols: mercaptoethanol, dithiothreitol, L-cysteine, glutathione

134 , urea, glycerol, ammonium sulfate, and beta-mercaptoethanol, do not interfere with this method.

135 tects the enzyme from inactivation, but beta-mercaptoethanol does not, suggesting that the 6 beta-pro

136 tects the enzyme from inactivation, but beta-mercaptoethanol does not, suggesting that these compound

137 ncentration of either dithiothreitol or beta-mercaptoethanol eliminated the background problems and a

138 ified a new spore protein, ExsM, from a beta-mercaptoethanol extract of B. cereus ATCC 4342 spores.

139 The moiety was present in the beta-mercaptoethanol extracts of cell walls from both blastoc

140 ation of microcystin-containing samples with mercaptoethanol, followed by LC-MS analysis, clearly dis

141 acetyl cysteine reacts similarly, while beta-mercaptoethanol gives equal amounts of 1,4 and 1,6 addit

142 2-Mercaptoethanol, glutathione, dithiothreitol, and thioph

143 other hand, neither dithiothreitol nor beta-mercaptoethanol had any effect on the N-SMase, suggestin

144 group, an external thiolate provided by beta-mercaptoethanol has likely been recruited to supply the

145 owed that [Asp(3), Dhb(7)]MC-RR reacted with mercaptoethanol hundreds of times more slowly than MC-RR

146 the presence of Co(2+), Mn(2+)or Mg(2+)and 2-mercaptoethanol in cacodylate or HEPES buffer, pH 7.2, e

147 from trapping of the intermediate with beta-mercaptoethanol in competition with hydride transfer fro

148 Inclusion of 2-mercaptoethanol in the upper electrode buffer greatly im

149 hiol reducing agents dithiothreitol and beta-mercaptoethanol inactivated the enzyme.

150 Ag(2+) and beta-mercaptoethanol increased while SDS and EDTA inhibited t

151 d from a phenylarsine oxide matrix with beta-mercaptoethanol indicating that they contain vicinal dit

152 e than the native protein in preventing beta-mercaptoethanol induced aggregation of insulin.

153 yo-electron microscopy and compare it to a B-mercaptoethanol-inhibited structure at 2.5 A resolution.

154 how that the use of a shorter diluent like 2-mercaptoethanol is more advantageous than using a longer

155 ctive aerobic oxidation of sulfur compounds (mercaptoethanol is oxidized to the corresponding disulfi

156 e radical abstracts hydrogen atoms from beta-mercaptoethanol (k = 8.8 +/- 0.5 x 10(6) M(-)(1) s(-)(1)

157 Low concentrations of the co-substrate beta-mercaptoethanol (Kd = approximately 13 mM) decrease the

158 To assess the efficacy of 2-mercaptoethanol/L-cysteine mixed disulfide (CySSME) as a

159 ming a thiol place-exchange reaction between mercaptoethanol (ME) attached to the Au nanostructures a

160 employing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) resulted in 39.5% and 29.5%, respec

161 , 2-(diethylamino)ethanethiol (DEAET), and 2-mercaptoethanol (ME) with o-phthaldialdehyde in the pres

162 molecules, such as dithiothreitol (DTT) and mercaptoethanol (ME), as release triggers.

163 rescued from l-Hcy-induced apoptosis by beta-mercaptoethanol medium supplementation that increases cy

164 bation of calf-thymus DNA with MA and DTT or mercaptoethanol (MER) resulted in the formation of MA-DN

165 sfully as co-substrates were thiosulphate, 2-mercaptoethanol, mercaptoacetate and aminoethylthiopseud

166 A structure with the loop occupied with beta-mercaptoethanol mimics binding of MAs(III).

167 ction species, as demonstrated with the beta-mercaptoethanol model.

168 lene) bridge facilitates the addition of two mercaptoethanol molecules as compared to the alkane brid

169 hibition by 3 was blunted by the presence of mercaptoethanol, no such protection was observed against

170 Hydrogen atom transfer to 1 from beta-mercaptoethanol occurs exclusively from the alpha-face t

171 Heating and reduction with 2-mercaptoethanol of GIF resulted in the release of a appr

172 erivatization with appropriate thiols (e.g., mercaptoethanol) of the olefin in the alpha,beta-unsatur

173 Lipophilic thiols such as mercaptoethanol or cysteamine could partially reverse th

174 On reduction with mercaptoethanol or dithiothreitol, the alpha chains of t

175 2+ > Mg2+; no inhibition was found with beta-mercaptoethanol or dithiothreitol.

176 tion of metal complexing agents such as beta-mercaptoethanol or imidazole to the reaction buffer.

177 syntheses between 3-chloroalanine and either mercaptoethanol or mercaptoacetate with equal facility,

178 rsh conditions such as treatment with pure 2-mercaptoethanol or treatment with boiling water for 5 h.

179 s were mixed with 300 x molar excess of beta-mercaptoethanol over the p-hydroxymercuribenzoate groups

180 mM phosphate buffer-1 mM Na2EDTA-47 mM beta-mercaptoethanol, pH 5.85 and then with 10 mM phosphate b

181 Pretreatment of corneas with 2-mercaptoethanol prevented the effects of Hg2+ on the par

182 Treatment of the labeled samples with beta-mercaptoethanol prior to SDS-PAGE removed the disulfide-

183 reparations, such as dithiothreitol and beta-mercaptoethanol, probably preserve enzyme activity by pr

184 other antioxidants, N-acetyl cysteine and 2-mercaptoethanol, reduced growth and viability of MO7e ce

185 dium dodecyl sulfate (SDS, surfactant), beta-mercaptoethanol (reducing agent) or ethylenediaminetetra

186 Treatment with beta-mercaptoethanol removed all radiolabel.

187 Supplementation of culture media with beta-mercaptoethanol rescues CD98hc-deficient cell survival.

188 igh molecular mass (>200 kDa), SDS- and beta-mercaptoethanol-resistant Fas aggregates were formed imm

189 Reduction of this disulfide bond by beta-mercaptoethanol restores activity, indicating that mobil

190 f a differentiation protocol containing beta-mercaptoethanol resulted in cells that expressed signifi

191 Mg(2+), Zn(2+), Cu(2+), K(1+), EDTA and beta-mercaptoethanol resulted in enhanced xylanase activity.

192 rtially protected via co-treatment with beta-mercaptoethanol, resulting in reduced disulfide bond for

193 reatment of the Co3+-PEPCK complex with beta-mercaptoethanol results in a loss of cobalt and full rec

194 Treatment of the complex with beta-mercaptoethanol results in near quantitative release of

195 s with reconstituted VcrA in the presence of mercaptoethanol revealed the presence of Cob(II)alamin.

196 In both instances, beta-mercaptoethanol reversed the inhibitory effects.

197 HIV-uninfected persons generated beta-mercaptoethanol-sensitive and -resistant antibodies to p

198 nd V(H)4 determinants were expressed by beta-mercaptoethanol-sensitive antibodies only; and HIV-infec

199 Moreover, human UBA3 could form a beta-mercaptoethanol-sensitive conjugate with NEDD8 in the pr

200 A beta-mercaptoethanol-sensitive Ubc9-sentrin conjugate could a

201 Reduction with beta-mercaptoethanol showed that the 70-kDa protein consists

202 The reducing agent 2-mercaptoethanol significantly attenuates filament assemb

203 500 mM NaCl, 20 mM Tris (pH 8.4), 2 mM beta-mercaptoethanol significantly enhances dimer formation w

204 Skim milk with low levels of added beta-mercaptoethanol (SM-ME) and untreated skim milk (SM) wer

205 compound 8a and subsequent treatment with 2-mercaptoethanol/sodium methoxide afforded the guanine an

206 At physiological pH, zinc and beta-mercaptoethanol stimulated the adenine DNA glycosylase a

207 Inhibition by dithiothreitol and beta-mercaptoethanol suggests that intramolecular disulfide b

208 The active site contains a molecule of beta-mercaptoethanol that is positioned between His-106 and A

209 Upon reduction with 2-mercaptoethanol the mass of this product decreased to 14

210 280 to 100 kDa, but in the presence of beta-mercaptoethanol the top of the DSP smear disappeared.

211 After selective elution with beta-mercaptoethanol, the peptides are sequenced using nanofl

212 f thiols, such as cysteine, glutathione, and mercaptoethanol, the yield of hydrogen sulfide release r

213 diates in the reaction of L-serine with beta-mercaptoethanol, they retain activity in the reaction of

214 Under reducing condition, beta-mercaptoethanol (thiol reducing agent) dissociated disul

215 me cysteine residues were derivatized with 2-mercaptoethanol to form S,S-(2-hydroxyethyl)thiocysteine

216 ons of amino acids with o-phthaldialdehyde/2-mercaptoethanol to generate electroactive derivatives th

217 The addition of the reducing agent beta-mercaptoethanol to samples prepared from METH-treated ra

218 e cross-linked complex was treated with beta-mercaptoethanol to transfer the 125I photomoiety from th

219 iation with ubiquitin was sensitive to beta2-mercaptoethanol, unlike lysine-ubiquitin bonds.

220 be incubated in 10 mM dithiothreitol or beta-mercaptoethanol until the precipitate is dissolved and t

221 2-Mercaptoethanol was a weak competitive inhibitor vs CoM

222 A preincubation with 2-mercaptoethanol was also included for optimal permeabili

223 hE(wt) stored in the presence of beta-mercaptoethanol was covalently modified at three cystein

224 f the isoindole derivative L-serine-NDA-beta-mercaptoethanol was found to follow pseudo-first order k

225 derived from factor V treated with 1 mm beta-mercaptoethanol was inactivated more rapidly than the un

226 When mercaptoethanol was used to reduce dehydroascorbic acid

227 otein from B. subtilis cells when urea and 2-mercaptoethanol were used in breakage buffers, implying

228 as acetate, and by thiols such as GSH and 2-mercaptoethanol, which disrupt its stabilizing double bo

229 his new assay uses 1,2-diacetyl benzene/beta-mercaptoethanol, which forms a fluorescent iso-indole-me

230 xchange that leads to the generation of beta-mercaptoethanol, which in turn decouples the conjugate a

231 presence of thiol nucleophiles such as beta-mercaptoethanol, which was used as a model, o-QMs are co

232 and FDH was independent of the presence of 2-mercaptoethanol while 10-FDDF dehydrogenase activity of

233 e radical abstracts hydrogen atoms from beta-mercaptoethanol with a bimolecular rate constant = 2.6 +

234 Replacing beta-mercaptoethanol with dithiothreitol in the loading buffe

235 n PBS but became fluorescent after SDS and 2-mercaptoethanol, with a quenching capacity of 10-fold fo

236 rogen peroxide or reducing the products with mercaptoethanol, with analysis by gas chromatography mas