ライフサイエンスコーパス: methionyl-tRNA

1 ractions with the anticodon of the initiator methionyl tRNA.

2 n protein synthesis are aminoacylated to non-methionyl-tRNAs.

3 somes, mRNA's, tRNA's including an initiator methionyl-tRNA, aminoacyl tRNA synthetases, and other pr

4 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40 S ribosomal subunit to

5 sential role in the binding of the initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to

6 esponsible for extensive misacylation of non-methionyl tRNAs, and mismethionylation also occurs in th

7 MetRS readily misacylates non-methionyl tRNAs at frequencies of up to 10% in mammalian

8 tion of 80 S ribosomes, stabilizes initiator methionyl-tRNA binding to 40 S subunits, and is required

9 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

10 The formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

11 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

12 Formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

13 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF;

14 It proposes that the 40S ribosome-methionyl-tRNA complex recognizes and binds to the 5'-en

15 uorescent probes were covalently attached to methionyl-tRNA(f) and tested for their incorporation int

16 he N-terminal domain is highly homologous to methionyl-tRNA(f)Met formyltransferase.

17 Met is misacylated to specific non-methionyl-tRNA families, and these Met-misacylated tRNAs

18 y believed to require a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation.

19 oplasts normally uses a formylated initiator methionyl-tRNA (fMet-tRNA(f)(Met)).

20 ation complexes (ICs) that carry an N-formyl-methionyl-tRNA (fMet-tRNA(fMet)).

21 y believed to require a formylated initiator methionyl-tRNA (fMet-tRNAfMet) in a process involving in

22 ss by limiting the availability of initiator methionyl-tRNA for translation.

23 shares 32% identity with Escherichia coli L-methionyl-tRNA formyltransferase (EC 2.1.2.9), was expre

24 hich the nuclear gene encoding mitochondrial methionyl-tRNA formyltransferase (FMT1) has been deleted

25 Concordantly, in vivo ablation of methionyl-tRNA formyltransferase (MTF) in Escherichia co

26 in an inducible manner the Escherichia coli methionyl-tRNA formyltransferase (MTF) in the cytoplasm

27 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

28 initiator methionyl-tRNA (Met-tRNA(Met)) by methionyl-tRNA formyltransferase (MTF) is important for

29 on of initiator methionyl-tRNA (Met-tRNA) by methionyl-tRNA formyltransferase (MTF) is important for

30 e formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

31 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

32 Formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

33 The formylation reaction is catalyzed by methionyl-tRNA formyltransferase (MTF) located in mitoch

34 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF; EC 2.1.2.9) is im

35 sary for synthesis of 10-formyl-THF, and the methionyl-tRNA formyltransferase (open reading frame YBL

36 ed at the FMT1 locus, encoding mitochondrial methionyl-tRNA formyltransferase, lack detectable fMet-t

37 li overproducing aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase, or IF2, we identified

38 e same Rossmann fold as the related enzymes, methionyl-tRNA-formyltransferase and glycinamide ribonuc

39 10-formyltetrahydrofolate dehydrogenase and methionyl-tRNA-formyltransferase extends to the C termin

40 16-aa insertion loop present in eubacterial methionyl-tRNA formyltransferases (MTF) is critical for

41 ast 18S rRNA critical in vivo for recruiting methionyl tRNA(i)(Met) to 40S subunits during initiation

42 ong with the initiator transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece

43 In vitro, eIF-2 binds the initiator methionyl-tRNA in a GTP-dependent fashion.

44 sults indicate that formylation of initiator methionyl-tRNA is not required for mitochondrial protein

45 ment distinguishing initiator from elongator methionyl tRNA, is required for recognition of the methi

46 n occur without formylation of the initiator methionyl-tRNA (Met-tRNA(fMet)).

47 een shown to direct binding of the initiator methionyl-tRNA (Met-tRNA(i)) to 40 S ribosomal subunits

48 N-Formylation of initiator methionyl-tRNA (Met-tRNA(Met)) by methionyl-tRNA formylt

49 The specific formylation of initiator methionyl-tRNA (Met-tRNA) by methionyl-tRNA formyltransf

50 s region of the E. coli enzyme and initiator methionyl-tRNA (Met-tRNA) by using two complementary pro

51 ic initiation factor 2 (eIF2), the initiator methionyl-tRNA (Met-tRNA), and GTP is a critical step in

52 eIF2), GTP, and methionine-charged initiator methionyl-tRNA (met-tRNAi).

53 n synthesis, a ribosome with bound initiator methionyl-tRNA must be assembled at the start codon of a

54 These include SAH hydrolase, methionyl-tRNA synthase, 5-methyltetrahydrofolate:Hcy me

55 ments of the anticodon for aminoacylation by methionyl tRNA synthetase and IleRS.

56 ective at creating negative determinants for methionyl tRNA synthetase and positive determinants for

57 Here we describe a mutant murine methionyl-tRNA synthetase (designated L274GMmMetRS) that

58 both of which are activated by an engineered methionyl-tRNA synthetase (designated NLL-MetRS), are ex

59 Human mitochondrial methionyl-tRNA synthetase (human mtMetRS) has been ident

60 e-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-

61 In one case, the C-terminal disruption of methionyl-tRNA synthetase (MetG) results in a 10,000-fol

62 a strain carrying a single genomic copy of a methionyl-tRNA synthetase (MetRS) gene, metG*, engineere

63 x with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm to re

64 ighly accurate, recent results show that the methionyl-tRNA synthetase (MetRS) is an exception.

65 aturation mutagenesis library of the E. coli methionyl-tRNA synthetase (MetRS) led to the discovery o

66 chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow eff

67 Methionyl-tRNA synthetase (MetRS) plays a crucial role i

68 The active site of methionyl-tRNA synthetase (MetRS) possesses two function

69 he centerpiece of the AND gate is a bisected methionyl-tRNA synthetase (MetRS) that charges the Met s

70 cylation of tRNA(Leu) with methionine by the methionyl-tRNA synthetase (MetRS).

71 anaerobiosis and antibiotic exposure via the methionyl-tRNA synthetase (MetRS).

72 s typically accomplished using an engineered methionyl-tRNA synthetase (MetRS-NLL).

73 In the work described here, human methionyl-tRNA synthetase (MRS) and human lysyl-tRNA syn

74 dified to lysidine to prevent recognition by methionyl-tRNA synthetase (MRS) and production of a chim

75 (ERS), glutaminyl-tRNA synthetase (QRS), and methionyl-tRNA synthetase (MRS), which were specifically

76 re, we identify alanyl-tRNA synthetase 1 and methionyl-tRNA synthetase 1 variants as new gene defects

77 Previously, we demonstrated that the class I methionyl-tRNA synthetase aminoacylates RNA microhelices

78 itroso-Hcy is in fact transferred to tRNA by methionyl-tRNA synthetase and incorporated into protein

79 plication of the carboxyl-terminal domain of methionyl-tRNA synthetase and may direct tRNA to the act

80 Model enzymes, such as methionyl-tRNA synthetase and trypsin, were inactivated

81 ere we describe a rationally designed mutant methionyl-tRNA synthetase containing two point substitut

82 istakenly selected in place of methionine by methionyl-tRNA synthetase during protein biosynthesis, w

83 NA microarrays and filter retention that the methionyl-tRNA synthetase enzyme from Escherichia coli (

84 surrogate that requires a mutant form of the methionyl-tRNA synthetase for activation.

85 identical to the carboxyl-terminal domain of methionyl-tRNA synthetase from Caenorhabditis elegans, a

86 ort that heterologous expression of a mutant methionyl-tRNA synthetase from Escherichia coli permits

87 thioester of homocysteine, is synthesized by methionyl-tRNA synthetase in all cell types.

88 Urea-based methionyl-tRNA synthetase inhibitors were designed, synt

89 ogate, azidohomoalanine, is activated by the methionyl-tRNA synthetase of Escherichia coli and replac

90 Homocysteine (Hcy) editing by methionyl-tRNA synthetase results in the formation of Hc

91 ve identified mutations in the mitochondrial methionyl-tRNA synthetase, Aats-met, the homologue of hu

92 Mes1p, methionyl-tRNA synthetase, also suppresses the defect in

93 n is our finding that the plant Oryza sativa methionyl-tRNA synthetase, expressed in Escherichia coli

94 siological buffer conditions with wheat germ methionyl-tRNA synthetase, required mutation of the anti

95 modifications formed via a pathway involving methionyl-tRNA synthetase-catalyzed metabolic conversion

96 em alone can be aminoacylated by the class I methionyl-tRNA synthetase.

97 cylated with methionine by overproduction of methionyl-tRNA synthetase.

98 of the respective gene products alanyl- and methionyl-tRNA synthetase.

99 tone in L. luteus, suggesting involvement of methionyl-tRNA synthetase.

100 ine concentration, suggesting involvement of methionyl-tRNA synthetase.

101 f the anticodon in aminoacylation of tRNA by methionyl-tRNA synthetase.

102 a similar base-pair uncoupling when bound to methionyl-tRNA synthetase.

103 Escherichia coli isoleucyl- and methionyl-tRNA synthetases are closely related enzymes t

104 s, aminoacyl-tRNA synthetases, including the methionyl-tRNA synthetases MetRS1 and MetRS2, are attrac

105 (eIF2) bound to GTP transfers the initiator methionyl tRNA to the 40S ribosomal subunit.

106 Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes

107 1- and eIF1A-dependent delivery of initiator methionyl-tRNA to the 40 S ribosomal subunit and subsequ

108 e dissociation, the binding of the initiator methionyl-tRNA to the 40 S ribosomal subunit, and mRNA r

109 d through modulation of binding of initiator methionyl-tRNA to the 40 S ribosomal subunit.

110 on initiation factor eIF2 delivers initiator methionyl-tRNA to the 40 S ribosomal subunit.

111 Events regulating the binding of initiator methionyl-tRNA to the 40S ribosomal subunit were assesse

112 erhaps to direct or stabilize the binding of methionyl-tRNA to the ribosomal P site.

113 facilitating the proper binding of initiator methionyl-tRNA to the ribosomal P site.

114 2B controls the recruitment of the initiator methionyl-tRNA to the ribosome and is activated by insul

115 nucleotide exchange to deliver the initiator methionyl-tRNA to the ribosome.

116 overproduction of lysyl-tRNA synthetase and methionyl-tRNA transformylase results in partial formyla

117 ectly to supporting or stabilizing initiator methionyl tRNA (tRNA-Met(i)) association with the riboso

118 in its GTP-bound state to deliver initiator methionyl-tRNA (tRNA(i)(Met)) to the small ribosomal sub

119 nary complex (TC) with GTP and the initiator methionyl-tRNA (tRNAi), mediating ribosomal recruitment

120 gh-copy-number IMT genes, encoding initiator methionyl tRNA (tRNAiMet), or LHP1, encoding the yeast h

121 strate that Escherichia coli misacylates non-methionyl-tRNAs with methionine in response to anaerobio