ライフサイエンスコーパス: methylguanosine

1 e methylation of the exocyclic N2 amine of 7-methylguanosine.

2 unmethylated counterparts, or nucleoside N7-methylguanosine.

3 guanosine, gamma-monomethyl phosphate, nor 7-methylguanosine.

4 lity of the 5'-5'-triphosphate bridge and N7-methylguanosine.

5 ne, inosine, xanthosine, pseudouridine, N(2)-methylguanosine, 1-methyladenosine, and N(2),N(2)-dimeth

6 ridine, was ~ tenfold as large as those of 1-methylguanosine, 1-methyladenosine, or 4-acetylcytidine,

7 ) and for 2'-O-methylcytidine (24%) and 2'-O-methylguanosine (22%) in 23S rRNA.

8 rategy for efficient synthesis for 2'-C-beta-methylguanosine (3).

9 nspecific binding of RNA, recognition of a 7-methylguanosine 5' mRNA cap, and methylation of a nuclei

10 of beta-D-2'-deoxy-2'-alpha-fluoro-2'-beta-C-methylguanosine-5'-monophosphate.

11 re we describe the synthesis of 8-nitro-2'-O-methylguanosine, a ribonucleoside analogue of this lesio

12 mical studies demonstrated that 8-nitro-2'-O-methylguanosine adopts a syn conformation about the glyc

13 thway, since mature tRNA(Val(AAC)) lacking 7-methylguanosine and 5-methylcytidine is rapidly degraded

14 se from Drosophila that cleaves m(7)GMP to 7-methylguanosine and inorganic phosphate.

15 es and previous crystallographic data for N7-methylguanosine and its phosphorylated derivatives, thes

16 The modified nucleosides N(2)-methylguanosine and N(2)(2)-dimethylguanosine in transfe

17 ganism were dihydrouridine, pseudouridine, 7-methylguanosine, and 5-methyluridine.

18 tly identified 2'-C-methyladenosine and 2'-C-methylguanosine as potent nucleoside inhibitors of HCV R

19 to frameshift suppressor tRNA(SufA6) and N1-methylguanosine at position 37 (m(1)G37) modification-de

20 The 7-methylguanosine cap added to the 5' end of mRNA is requi

21 rand RNA virus that lacks the canonical 5' 7-methylguanosine cap and a 3' poly-A tail.

22 cognize and productively interact with the 7-methylguanosine cap at the 5' end of the mRNA and subseq

23 ctions that result in the formation of an N7-methylguanosine cap during mRNA maturation.

24 The 5' N(7)-methylguanosine cap is a critical modification for mRNAs

25 A 5',7-methylguanosine cap is a quintessential feature of RNA p

26 mRNAs are appended at the 5' end, with the 7-methylguanosine cap linked by a 5'-5'-triphosphate bridg

27 ex, made up of eIF4E, which recognizes the 7-methylguanosine cap of messenger RNA, and eIF4G, which s

28 initiation factor 4E (eIF4E) binds to the 7-methylguanosine cap of mRNA and facilitates binding of m

29 sphorylation subsequent to addition of the 7-methylguanosine cap on pre-mRNA in a manner that facilit

30 The cotranscriptional placement of the 7-methylguanosine cap on pre-mRNA is mediated by recruitme

31 20 and CBP80, respectively) that binds the 7-methylguanosine cap on RNAs transcribed by RNA polymeras

32 of protein synthesis, the mRNA 5'-terminal 7-methylguanosine cap structure and several recognition pr

33 The 7-methylguanosine cap structure at the 5' end of eukaryoti

34 f an mRNA through its interaction with the 7-methylguanosine cap, and it subsequently scans along the

35 eIF4A is part of the 5'-7-methylguanosine cap-binding complex, eIF4F, along with e

36 f the translational repressor 4EBP1 to the 7-methylguanosine cap-binding complex.

37 e first encoded nucleotide adjacent to the 7-methylguanosine cap.

38 in eukaryotes requires the addition of the 7-methylguanosine cap.

39 on initiation independent of the 5' end N(7)-methylguanosine cap.

40 transcriptional 5'-end modification with a 7-methylguanosine cap.

41 In eukaryotes, the 5'-methylguanosine (cap) structure is principally removed b

42 e gene promoters was introduced along with 7-methylguanosine capped RNAs encoding piggyBac transposas

43 this study, we discovered and characterized methylguanosine-capped and polyadenylated small RNAs (CP

44 igh affinity variant of eIF-4E to capture 5'-methylguanosine-capped RNA followed by 3'-RACE sequencin

45 NA is transcribed by RNA polymerase II and 7-methylguanosine-capped, binds the seven Sm proteins, bec

46 y related to mammalian eIF4E-1, binds only 7-methylguanosine caps and is essential for viability.

47 iardia mRNAs have blocked 5'-ends and that 7-methylguanosine caps promote translation of transfected

48 ow that aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but is not adept at removing a deo

49 IFE-4, which binds only 7-methylguanosine caps, is most closely related to an unus

50 Fifty-eight analogues of the 5'-terminal 7-methylguanosine-containing cap of eukaryotic messenger R

51 ding of the initiation factor eIF4E to the 7-methylguanosine-containing cap of mRNAs.

52 truct bearing a conventional cap analogue (7-methylguanosine) failed to produce ITGA4 protein, but ex

53 able substrate and replacement of dG by 2'-O-methylguanosine generated a substrate with a low specifi

54 The terminal 7-methylguanosine is recognized by cap-binding proteins th

55 The minimal 'cap0' consists of N7-methylguanosine linked to the first nucleotide via a 5'-

56 uch as N(1) -methyladenosine (m(1) A), N(1) -methylguanosine (m(1) G), N(3) -methylcytosine (m(3) C),

57 her analysis showed the accumulation of N(1)-methylguanosine (m(1)G(37)) in tRNA from cells bearing a

58 e (m(1)A), N(3)-methylcytidine (m(3)C), N(1)-methylguanosine (m(1)G) and N(2),N(2)-dimethylguanosine

59 TRMT10A installs N (1)-methylguanosine (m(1)G) in tRNA, and FTO performs demeth

60 ron microscopy, we demonstrate that the N(1)-methylguanosine (m(1)G) modification at position 37 of E

61 m(1)A), N(3)-methylcytidine (m(3)C) and N(1)-methylguanosine (m(1)G), all commonly found in tRNAs.

62 ively converts m(2)2 G modification to N(2) -methylguanosine (m(2) G).

63 tion, a common methylation mark seen in N(2)-methylguanosine (m(2)G) and N(2),N(2)-dimethylguanosine

64 7-Methylguanosine (m(7)G) at tRNA position 46 is a conserv

65 located adjacent to the 5'-end of the mRNA 7-methylguanosine (m(7)G) cap structure.

66 Uniquely, the sfTR transcript harbors a 5'-7-methylguanosine (M(7)G) cap, as opposed to the more typi

67 A Pol II are modified at the 5' end with a 7-methylguanosine (m(7)G) cap, which is recognized by the

68 e (DAP), 7-deazaguanosine (7-deaza-G), and 7-methylguanosine (m(7)G) diphosphates efficiently accepte

69 is unique among eukaryotes and consists of 7-methylguanosine (m(7)G) followed by four methylated nucl

70 N(7)-methylguanosine (m(7)G) introduced at position 1575 on 1

71 N(7)-methylguanosine (m(7)G) is a positively charged, essenti

72 diverse collection of tRNA modifications, 7-methylguanosine (m(7)G) is frequently found in the tRNA

73 N(7)-methylguanosine (m(7)G) is required for integrity and st

74 WDR4, a tRNA-binding cofactor of the N(7)-methylguanosine (m(7)G) methyltransferase complex, remai

75 s of the N6-methyladenosine (m(6)A) versus 7-methylguanosine (m(7)G) modification in polyA+-purified

76 he RNA methyltransferase METTL1 catalyzes N7-methylguanosine (m(7)G) modification of tRNAs.

77 N(7)-methylguanosine (m(7)G) modification, routinely occurrin

78 Here, we show that the transfer RNA N(7)-methylguanosine (m(7)G) transferase METTL1 is highly exp

79 7-Methylguanosine (m(7)G), also known as the mRNA "cap", i

80 y during transcription by the addition of N7-methylguanosine (m(7)G), which forms the "cap" on the fi

81 re, we document microprocessor-independent 7-methylguanosine (m(7)G)-capped pre-miRNAs, whose 5' ends

82 lity of a monoclonal antibody to recognize 7-methylguanosine (m(7)G).

83 ment located immediately downstream of the 7-methylguanosine [m(7)G] cap of TOP mRNAs, which encode r

84 reviously known hyperactive mutant toward N1-methylguanosine (m1G) in RNA.

85 ansferase 5 (TRMT5)-mediated formation of N1-methylguanosine (m1G) in the transfer RNA (tRNA) anticod

86 In addition, the novel ser-tRNACAG has 1-methylguanosine (m1G-37) at position 37, 3' to the antic

87 germ extract translation systems, whereas N2-methylguanosine (m2G) moderately impeded translation.

88 e show deposition of one RNA modification-N2-methylguanosine (m2G) on the G72 of U6 snRNA (the cataly

89 To investigate this possibility for N 2-methylguanosine (m2G), which is present in a wide variet

90 ) did not perturb translational fidelity, O6-methylguanosine (m6G) at the first and second codon posi

91 bed by RNA polymerase II and their initial 7-methylguanosine (m7G) 5' cap structures subsequently bec

92 Mammalian mRNAs possess an N7-methylguanosine (m7G) cap and 2'O methylation of the ini

93 In eukaryotes, a 7-methylguanosine (m7G) cap is added to newly transcribed

94 ny eukaryotic viruses, contain an inverted 7-methylguanosine (m7G) cap linked to the 5' nucleotide of

95 The formation of the 5' 7-methylguanosine (m7G) cap structure is known to require

96 to be the first factor to bind mRNA during 7-methylguanosine (m7G) cap-dependent translation initiati

97 nscripts being modified by addition of the 7-methylguanosine (m7G) cap.

98 7-methylguanosine (m7G) is present at mRNA caps and at def

99 With recent progress in mapping N7-methylguanosine (m7G) RNA methylation sites, tens of tho

100 Recent progress in N7-methylguanosine (m7G) RNA methylation studies has focuse

101 One example is the N1-methylguanosine modification at guanine nucleotide 37 (m

102 he orthologue of trm8, which catalyses the 7-methylguanosine modification of tRNA in Saccharomyces ce

103 modification, locked nucleic acid (LNA) N(7)-methylguanosine modifications on the cap and LNA + 5 x 2

104 e methylated cap nucleotide in the form of 7-methylguanosine monophosphate (m(7)GMP) or diphosphate (

105 P39 with a genuine nucleobase analogue of N7-methylguanosine, namely, N7,9-dimethylguanine, indicated

106 We also applied our method to O6-methylguanosine (O6mG) modified DNA substrates and ident

107 2'-C-Methylguanosine, on the other hand, was neither efficien

108 triphosphate, cap0 (triphosphate-bridged N7-methylguanosine), or cap1 (cap0 with RNA 2'-O-methylatio

109 ross-resistance exists between the 2'-F-2'-C-methylguanosine prodrugs and other classes of HCV inhibi

110 Moreover, the amplitude of the 1-methylguanosine rhythm was correlated with the rest-acti

111 1 is the enzyme responsible for converting 7-methylguanosine RNA caps to the 2,2,7-trimethylguanosine

112 N7,9-dimethylguanine, indicated that the N7-methylguanosine rotational orientation within the stack

113 snoRNAs) undergoes hypermethylation from a 7-methylguanosine to a 2,2, 7-trimethylguanosine structure

114 ne nucleoside phosphorylase with substrate 7-methylguanosine to reduce the calculated internal [Pi] i

115 we report the synthesis of 5'-O-(1-thio)-N2-methylguanosine triphosphate (m2GalphaS) and its incorpo

116 igand binding between VPg and anthraniloyl-7-methylguanosine triphosphate for eIFiso4E.

117 2'-C-Methylguanosine triphosphate has been known as a potent

118 2'-C-Methyladenosine and 2'-C-methylguanosine were identified as potent inhibitors of