ライフサイエンスコーパス: micrococcal

1 We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (Tu

2 c mRNA length) were found to be resistant to micrococcal nuclease (69%) or to remain suspended in ass

3 nization but were found to be susceptible to micrococcal nuclease (85%) or to sediment to a pellet in

4 ergence between different Hi-C protocols and micrococcal nuclease (micro-C).

5 r, since the activities in the extracts were micrococcal nuclease (MN) sensitive.

6 scription elongation complex to digestion by micrococcal nuclease (MN).

7 activity of the S. aureus secreted nuclease, micrococcal nuclease (MN).

8 ribosome integrity but negatively impact the micrococcal nuclease (MNase) activity, necessitating usi

9 ly in situ single cell chromatin imaging and micrococcal nuclease (MNase) assay to show that Brd4 dep

10 ructure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal

11 Chromatin immunoprecipitation (ChIP) and micrococcal nuclease (MNase) digest assays were performe

12 We combined low and high levels of micrococcal nuclease (MNase) digestion along with core h

13 accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (ass

14 uch as chromatin immunoprecipitation (ChIP), micrococcal nuclease (MNase) digestion and DNase I diges

15 We performed a time-course of micrococcal nuclease (MNase) digestion and measured the

16 emodelling analysis at gene promoters, using micrococcal nuclease (MNase) digestion followed by deep

17 We have used micrococcal nuclease (MNase) digestion followed by deep

18 We introduce a metric that uses micrococcal nuclease (MNase) digestion in a novel manner

19 We have combined standard micrococcal nuclease (MNase) digestion of nuclei with a

20 age-sensitive mtDNA regions were examined by micrococcal nuclease (MNase) digestion sequencing and Ln

21 erential nuclease sensitivity assay based on micrococcal nuclease (MNase) digestion to discover open

22 al DNA was significantly less protected from micrococcal nuclease (MNase) digestion up to 6 h postinf

23 erepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease i

24 for differential sensitivity of chromatin to micrococcal nuclease (MNase) digestion, we profile acces

25 Micrococcal nuclease (MNase) digests the linker to yield

26 Micrococcal nuclease (MNase) is commonly used to map nuc

27 Micrococcal nuclease (MNase) is extensively used in geno

28 Micrococcal nuclease (MNase) is widely used to map nucle

29 ely digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase

30 re-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different ce

31 Micrococcal nuclease (MNase) sequence-based profiles of

32 Digestion of the chromatin with micrococcal nuclease (MNase) shows a nucleosome array wi

33 ome-wide mapping of nucleosomes generated by micrococcal nuclease (MNase) suggests that yeast promote

34 method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucl

35 avage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice

36 hEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent

37 specific and highly efficient biosensor for micrococcal nuclease (MNase), an endonuclease produced b

38 Like micrococcal nuclease (MNase), MPE-Fe(II) preferentially

39 the restriction enzymes in Hi-C assays with micrococcal nuclease (MNase), resulting in capturing nuc

40 agments caused by the known sequence bias of micrococcal nuclease (MNase), the most widely used nucle

41 p Region Capture Micro-C (RCMC) by combining micrococcal nuclease (MNase)-based 3C with a tiling regi

42 In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemi

43 Here, using the micrococcal nuclease (MNase)-based chromatin accessibili

44 Using micrococcal nuclease (MNase)-based chromatin occupancy p

45 We used micrococcal nuclease (MNase)-based chromatin occupancy p

46 Compared to micrococcal nuclease (MNase)-based methods that map nucl

47 bind one or both full nucleosomes that flank micrococcal nuclease (MNase)-defined nucleosome-free pro

48 Enrichment of short micrococcal nuclease (MNase)-protected DNA segments indi

49 tes at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites.

50 inaccessible to DNA-binding factors, such as micrococcal nuclease (MNase).

51 e extent to which chromatin is digested with micrococcal nuclease (MNase).

52 n these mutants has reduced accessibility to Micrococcal nuclease (MNase).

53 antibody, followed by binding of a protein A-Micrococcal Nuclease (pA/MNase) fusion protein (Skene an

54 Pretreatment of the U1 snRNP with micrococcal nuclease abolished the assembly of galectin-

55 High-resolution micrococcal nuclease analyses revealed that a positioned

56 Micrococcal nuclease analysis revealed that the heteroch

57 sults demonstrate increased DNA laddering by micrococcal nuclease and an increased amount of DNA inte

58 units are equally accessible to DNase I and micrococcal nuclease and contain similar levels of histo

59 n vitro and in vivo assays of sensitivity to micrococcal nuclease and dam methyltransferase, respecti

60 tivity of wild-type nuclei to digestion with micrococcal nuclease and deoxyribonuclease I, indicating

61 ecreased accessibility of their chromatin to micrococcal nuclease and DNase I digestion and increased

62 s of chromatin structure by accessibility to micrococcal nuclease and DNase I digestion demonstrated

63 core region of the insulator as revealed by micrococcal nuclease and DNase I digestion studies.

64 T118-I) are more accessible to digestion by micrococcal nuclease and do not constrain DNA in a preci

65 n mitoribosome footprints are generated with micrococcal nuclease and mitoribosomes are separated fro

66 s with defined ends, bulk NCPs prepared with micrococcal nuclease and molecular modelling to reassess

67 s no hypersensitivity to either DNase I or a micrococcal nuclease and no translational positioning of

68 complex, as supported by its sensitivity to micrococcal nuclease and proteinase K.

69 e of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking.

70 undaries were determined by assays combining micrococcal nuclease and restriction endonuclease digest

71 region for several days, as demonstrated by micrococcal nuclease and restriction enzyme accessibilit

72 tuted mononucleosomes using exonuclease III, micrococcal nuclease and restriction enzymes demonstrate

73 Micrococcal nuclease and short-recognition-sequence blun

74 erformance liquid chromatography analysis of micrococcal nuclease and spleen phosphodiesterase-digest

75 In micrococcal nuclease and supercoiling assays, addition o

76 eabilization and digestion of chromatin with micrococcal nuclease and then compared tumor necrosis fa

77 e flexibility and strongly blocked access of micrococcal nuclease as contour lengths shortened, consi

78 ain reaction, site-directed mutagenesis, and micrococcal nuclease assay to determine the role of S-ni

79 Gene expression profiling, micrococcal nuclease assay, and chromatin immunoprecipit

80 and increased mononucleosome generation in a micrococcal nuclease assay.

81 ccessibility, as indicated by results of the micrococcal nuclease assay.

82 Chromatin immunoprecipitation and micrococcal nuclease assays were performed to determine

83 om human metaphase chromosomes digested with micrococcal nuclease associate spontaneously forming mul

84 The RTC becomes permeable to micrococcal nuclease but not to antibodies.

85 ent types of nucleosome remodeling events in micrococcal nuclease ChIP-seq (chromatin immunoprecipita

86 osome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to

87 itional perturbation is marked by changes in micrococcal nuclease cleavage patterns, restriction endo

88 chromatin nor to differences in the in vivo micrococcal nuclease cleavage sites in individual genes

89 10-bp periodicity in WW dinucleotides and in micrococcal nuclease cleavage, providing evidence for ro

90 caused by either linker histone addition or micrococcal nuclease cleavage.

91 clei sorting; 3) preparation of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-

92 The protection against micrococcal nuclease digestion afforded to chromatosomal

93 gradient sedimentation, thermal disassembly, micrococcal nuclease digestion and atomic force microsco

94 examined HSV-1 during lytic infection using micrococcal nuclease digestion and chromatin immunopreci

95 of S. cerevisiae nucleosome lengths based on micrococcal nuclease digestion and paired-end sequencing

96 er DNA, stabilizing an additional 20 bp from micrococcal nuclease digestion and restrict nucleosome m

97 e demonstrate by single-molecule approaches, micrococcal nuclease digestion and small-angle X-ray sca

98 e center of the DNA sequence, protected from micrococcal nuclease digestion by incorporation into a p

99 HBc 149, 154, and 157) remained intact after micrococcal nuclease digestion by native gel electrophor

100 region of the capsid pgRNA is susceptible to micrococcal nuclease digestion during its isolation and

101 replicative aging using spike-in controlled micrococcal nuclease digestion followed by sequencing.

102 ammalian linker histone H1 and have a unique micrococcal nuclease digestion footprint that allows the

103 ound histone H3 and increased sensitivity to micrococcal nuclease digestion in WHS patient-derived ce

104 Partial micrococcal nuclease digestion indicated that the sequen

105 Micrococcal nuclease digestion indicated the presence of

106 itivity of bulk chromatin from sin4 cells to micrococcal nuclease digestion is strikingly increased r

107 Micrococcal nuclease digestion of active promoters in nu

108 of genomic DNA species, produced by partial micrococcal nuclease digestion of chromatin, can be sequ

109 me-length DNA ( approximately 166 bp) during micrococcal nuclease digestion of chromatin.

110 of a 166 bp chromatosome intermediate during micrococcal nuclease digestion of chromatin.

111 itation, we developed a novel strategy using micrococcal nuclease digestion of cross-linked chromatin

112 Capsid disassembly can be induced by micrococcal nuclease digestion of encapsidated RNA.

113 445 nucleotide human telomerase RNA (hTR) by micrococcal nuclease digestion of partially purified hum

114 In this study, we use a procedure based on micrococcal nuclease digestion of reconstituted nucleoso

115 The micrococcal nuclease digestion pattern of chromatin from

116 pause at approximately 168 base pairs in the micrococcal nuclease digestion pattern of the chromatin.

117 ure on the transient template as measured by micrococcal nuclease digestion pattern.

118 rther, we found that A-T cells had different micrococcal nuclease digestion patterns compared to norm

119 structure as DNaseI-hypersensitive sites or micrococcal nuclease digestion patterns.

120 Despite these changes, the micrococcal nuclease digestion profile of this promoter

121 from the Rb-/- cells is more susceptible to micrococcal nuclease digestion than that from Rb+/+ fibr

122 the exon 1 region is much more sensitive to micrococcal nuclease digestion than the exon 2 and exon

123 A major site of micrococcal nuclease digestion was identified by mapping

124 obes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove a

125 Similar kinetics of micrococcal nuclease digestion were seen for all three t

126 nucleosome position in follicle cells using micrococcal nuclease digestion with Ilumina sequencing.

127 and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves

128 coli chromosome in vivo and protect DNA from micrococcal nuclease digestion, allowing us to map bindi

129 leosomes, produce a chromatosome stop during micrococcal nuclease digestion, and aggregate chromatin.

130 NA and multiples of approximately 60 bp from micrococcal nuclease digestion, and immunoprecipitation

131 nucleosomes prepared by partial and maximum micrococcal nuclease digestion, coupled with Western blo

132 niques along with such laboratory methods as micrococcal nuclease digestion, predicting the genomic l

133 H3 nucleosomes protect 90-100 bp of DNA from micrococcal nuclease digestion, sufficient for only a si

134 some maps generated by chemical cleavage and micrococcal nuclease digestion, the chemical map shows c

135 Using micrococcal nuclease digestion, we probed the chromatin

136 ved from K562 human erythroleukemic cells by micrococcal nuclease digestion.

137 (HTa) that protects part of the genome from micrococcal nuclease digestion.

138 . thermophila mononucleosomes were stable to micrococcal nuclease digestion.

139 ity, and chromatin that is hypersensitive to micrococcal nuclease digestion.

140 ome segment protected by the polymerase from micrococcal nuclease digestion.

141 CTD antibodies from chromatin solubilized by micrococcal nuclease digestion.

142 chromatin results in increased resistance to micrococcal nuclease digestion.

143 size differences between repeats in partial micrococcal nuclease digests and by trypsin treatment of

144 Analysis of micrococcal nuclease digests of chromatin using hybridiz

145 a typical pattern of nucleosomal repeats in micrococcal nuclease digests, the Tec element chromatin

146 by Snf-Swi, by a high resolution analysis of micrococcal nuclease digests.

147 rmal global chromatin density as assessed by micrococcal nuclease digests; and expressed normal level

148 e)--a protein containing five staphylococcal/micrococcal nuclease domains and a tudor domain--is a co

149 Pretreatment of nuclear extracts with micrococcal nuclease eliminated the phosphorylation of C

150 tivity () was solubilized by Triton X-100 or micrococcal nuclease extraction, whereas hTSH2B was rela

151 osomal protections in Drosophila cells using Micrococcal Nuclease followed by sequencing.

152 Micrococcal nuclease footprinting shows that after track

153 DNase I and micrococcal nuclease footprinting, of both 5' and 3' 32P

154 examined salt-soluble chromatin released by micrococcal nuclease from a 15-day-old chicken embryo er

155 ested chromatin from untreated rats revealed micrococcal nuclease hypersensitive regions in the proxi

156 The TBP gene comprises a 220 bp micrococcal nuclease hypersensitive site corresponding t

157 ional responses to cold tend to contain more micrococcal nuclease hypersensitive sites in their promo

158 ma regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the

159 Both hotspot sequences were sites of micrococcal nuclease hypersensitivity in meiotic chromat

160 Micrococcal nuclease hypersensitivity in the PBRU was la

161 We report DNase I and micrococcal nuclease hypersensitivity, chromatin immunop

162 The chromatin accessibility to micrococcal nuclease in the MFA2 promoter is unaffected

163 P knockout) brain homogenate with RNase A or micrococcal nuclease inhibited hamster but not mouse PrP

164 ocalization of Cse4 in chromatin digested by micrococcal nuclease is consistent with the potential as

165 cribe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzy

166 reconstituted branch migration substrates by micrococcal nuclease mapping and exonuclease III and hyd

167 High-resolution micrococcal nuclease mapping showed that ZL0580 induces

168 Chromatin immunoprecipitation and micrococcal nuclease mapping studies reveal that Knirps

169 Micrococcal nuclease mapping studies revealed that a nuc

170 With high-resolution micrococcal nuclease mapping, we show that the HMRa locu

171 The micrococcal nuclease Nuc1 is one of the major S. aureus

172 Treatment with micrococcal nuclease of highly purified mtRNase P confir

173 products, similar to the well-characterized micrococcal nuclease of Staphylococcus aureus.

174 moderately greater sequence preference than micrococcal nuclease or DNase I, and the sites attacked

175 essed by sedimentation and by digestion with micrococcal nuclease or DNase I.

176 prepared from chromatin digested with either micrococcal nuclease or DNaseI and are restricted in the

177 th Cse4 and H2A are precisely protected from micrococcal nuclease over the entire CDE of all 16 yeast

178 Micrococcal nuclease partial digestion generated a ladde

179 Treatment of the polyribosomes with micrococcal nuclease prior to salt extraction solubilize

180 Use of DNase I and micrococcal nuclease probes further indicated that the s

181 DNase I and micrococcal nuclease probes show that GATA-1 binding cau

182 nt stagger is explained by the finding that micrococcal nuclease produces NCPs not with flush ends,

183 Micrococcal nuclease protection analysis showed that les

184 ich antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA compl

185 this probe by the secreted S. aureus enzyme micrococcal nuclease results in emission of a readily de

186 ive and inactive CEN chromatin digested with micrococcal nuclease revealed that periodic nucleosome a

187 e of exons 1, 2, and 5 of the DHFR gene with micrococcal nuclease revealed that the exon 1 region is

188 PELP1 overexpression increased the micrococcal nuclease sensitivity of estrogen response el

189 This protocol describes single-cell micrococcal nuclease sequencing (scMNase-seq), a method

190 mics simulations based on publicly available micrococcal nuclease sequencing data for nucleosome posi

191 Micrococcal nuclease studies revealed that TRs are embed

192 Consistently, micrococcal nuclease susceptibility analysis revealed al

193 pressed CFS, the FRA3B, is more resistant to micrococcal nuclease than that of the flanking non-fragi

194 Consistently, the accessibility of micrococcal nuclease to chromatin was generally inhibite

195 a cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P R

196 riments where the extracts were treated with micrococcal nuclease to digest endogenous snRNAs, the ef

197 acO/GFP-LacI plants were lysed, treated with micrococcal nuclease to digest the DNA to fragments of a

198 one-DNA complexes using an antibody-targeted micrococcal nuclease to release DNA complexes for paired

199 that the 3' ends of capsid pgRNA isolated by micrococcal nuclease treatment are heterogeneously dispe

200 ted by using an established method involving micrococcal nuclease treatment demonstrated reduced leve

201 as not bridged by nucleic acids, as shown by micrococcal nuclease treatment of the proteins prior to

202 (1mg), and provides the option to use MNase (micrococcal nuclease) for chromatin fragmentation.

203 eosome cores were liberated using an enzyme (micrococcal nuclease) with a strong preference for cleav

204 vage activity is sensitive to treatment with micrococcal nuclease, also consistent with an activity a

205 Linker DNA was freely accessible to micrococcal nuclease, although the oligomers remained pa

206 digestion by DNAse, restriction enzymes, and micrococcal nuclease, and an increased affinity for GAL4

207 DNase I, micrococcal nuclease, and exonuclease III footprinting s

208 By combining DNase I, micrococcal nuclease, and specific restriction endonucle

209 atin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppr

210 Micrococcal nuclease, DNase I, and restriction enzymes s

211 f nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and restriction enzymes.

212 s associated with increased accessibility to micrococcal nuclease, i.e. nucleosome depletion.

213 histone promoters and transcribed regions to micrococcal nuclease, implicating UBTF1/2 in mediating D

214 DNA and stabilize it against digestion with micrococcal nuclease, in a similar manner to histone H1.

215 fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumpt

216 ith increased resistance to both DNase I and micrococcal nuclease, indicating that the silenced state

217 mic DNA is either sonicated or digested with micrococcal nuclease, making it possible that current pr

218 chromatic sequences become hypersensitive to micrococcal nuclease, nucleoli fail to form, and transcr

219 deed induce sites hypersensitive to DNase I, micrococcal nuclease, or restriction enzymes on either s

220 be produced by pretreatment with DNase I or micrococcal nuclease, respectively.

221 table for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by imm

222 A (vRNA) has been depleted by treatment with micrococcal nuclease, was used to study transcription in

223 By fusing a subunit of Mcm to micrococcal nuclease, we previously showed that known or

224 By using micrococcal nuclease, which has both endo- and exo-nucle

225 This Asf1p/H3/H4 complex generated micrococcal nuclease--resistant DNA in the absence of DN

226 organization at subnucleosome resolution by micrococcal nuclease-based chromosome conformation captu

227 staining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that E

228 we report single-cell Micro-C (scMicro-C), a micrococcal nuclease-based three-dimensional (3D) genome

229 Separation of micrococcal nuclease-digested chromatin by sucrose gradi

230 Low resolution Southern analysis of micrococcal nuclease-digested chromatin from untreated r

231 We begin with micrococcal nuclease-digested non-cross-linked chromatin

232 letion of both tails, a lethal event, alters micrococcal nuclease-generated nucleosomal ladders, plas

233 adjacent to the TATAA box and an additional micrococcal nuclease-hypersensitive site in the linker D

234 The position and intensity of micrococcal nuclease-hypersensitive sites correlate poor

235 d Sik1 promoter accessibility as measured by micrococcal nuclease-quantitative PCR and impaired histo

236 ent protein-tagged H1 variants, we show that micrococcal nuclease-resistant chromatin is specifically

237 ift assay, this protein produced a discrete, micrococcal nuclease-resistant complex with an approxima

238 Characterization of the most micrococcal nuclease-resistant DNA indicates that micron

239 visiae, obtained by paired-end sequencing of micrococcal nuclease-resistant DNA.

240 s that the centromeric nucleosome contains a micrococcal nuclease-resistant kernel of 123-135 bp, dep

241 ementing activity was protease-sensitive and micrococcal nuclease-resistant, had a native molecular m

242 der ( 150 bp) NDRs instead contain unstable, micrococcal nuclease-sensitive ("fragile") nucleosomal p

243 nctional significance of this association, a micrococcal nuclease-sensitive component, i.e., an snRNP

244 he human protamine cluster were subjected to Micrococcal Nuclease-seq.

245 Micrococcal nuclease-treated cell extracts are fully com

246 Using micrococcal nuclease-treated chromatin, we find that Cse

247 ng to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin.

248 by incubating human 32P-labeled U2 snRNP in micrococcal nuclease-treated HeLa nuclear extracts, foll

249 Addition of DNA to micrococcal nuclease-treated samples restored interactio

250 Micrococcal nuclease-treated TAP preparations were devoi

251 tects approximately 11 bp of linker DNA from micrococcal nuclease.

252 p of DNA is protected against digestion with micrococcal nuclease.

253 bited by a specific competitive inhibitor of micrococcal nuclease.

254 DNA fragments resulting from digestions with micrococcal nuclease.

255 the sensitivity of chromatin to digestion by micrococcal nuclease.

256 al DNA ladders sharper than those created by micrococcal nuclease.

257 d treatment of lysates with Ca(2+)-dependent micrococcal nuclease.

258 s even after digestion of the chromatin with micrococcal nuclease.

259 component is sensitive to cycloheximide and micrococcal nuclease.

260 iation to treatment with ethidium bromide or micrococcal nuclease.

261 decreased rate of digestion of chromatin by micrococcal nuclease.

262 ated by treatment with proteinase K, but not micrococcal nuclease.

263 leosomal DNA to Dam methyltransferase and to micrococcal nuclease.

264 the primary chromatin structure is probed by micrococcal nuclease.

265 sed sensitivity of chromatin to digestion by micrococcal nuclease; however, phosphorylation of H2A an

266 In micrococcal-nuclease digestion experiments, nucleosomes

267 erstood, including several studies utilizing micrococcal-nuclease sequencing (MNase-seq) methodologie