ライフサイエンスコーパス: microdilution

1 lution) to 98.6% (agar dilution versus broth microdilution).

2 crobial susceptibility was measured by broth microdilution.

3 s for 128 A. urinae isolates tested by broth microdilution.

4 xacin susceptibility was determined by broth microdilution.

5 tested for antimicrobial resistance by broth microdilution.

6 ed for antibiotic susceptibility using broth microdilution.

7 present in combination when tested by broth microdilution.

8 pared the results to those obtained by broth microdilution.

9 by the Etest (P < 0.00007) but not by broth microdilution.

10 roth macrodilution were susceptible by broth microdilution.

11 testing was conducted using automated broth microdilution.

12 compared to the results for reference broth microdilution.

13 as similarly poor for calcium-enhanced broth microdilution.

14 25 and 100%, respectively, compared to broth microdilution.

15 MICs were determined via broth microdilution.

16 s clinical isolates were determined by broth microdilution.

17 cavenging activity, disc-diffusion and broth microdilution.

18 tional caMHB and zinc-limited media by broth microdilution.

19 Based on broth microdilution, 0%, 2.2%, and 97.8% of the KPC isolates w

20 n resistance determination compared to broth microdilution (16 to 20 h), our study indicates that it

21 By broth microdilution, 43/44 invasive serotype 6C isolates were

22 rrent growth-based AST assays, such as broth microdilution(5), require several days before informing

23 ely, were as follows: oxacillin MIC by broth microdilution, 94.4% and 96.7%; oxacillin screen agar, 9

24 of oxacillin previously determined by broth microdilution according to CLSI guidelines.

25 MICs were determined using broth microdilution according to the CLSI reference method M27

26 antibiotic classes were determined by broth microdilution according to the guidelines of the Clinica

27 All isolates were MIC tested by broth microdilution against ciprofloxacin, levofloxacin, and o

28 imal inhibitory concentration (MIC) by broth microdilution against Mycobacterium smegmatis as a funct

29 sus result of three reference methods: broth microdilution, agar dilution, and disk diffusion.

30 solates to doripenem was determined by broth microdilution, agar dilution, and Etest.

31 inis, and Ureaplasma urealyticum using broth microdilution and agar dilution techniques.

32 day 1 and 2 thresholds for AUC/MIC by broth microdilution and AUC/MIC by Etest.

33 ., including MDR strains, by reference broth microdilution and disk diffusion (15-mug disk content) m

34 estis was evaluated in comparison with broth microdilution and disk diffusion for eight agents.

35 s tablet assay to both reference M38-A broth microdilution and disk diffusion methods for testing the

36 n = 112) were susceptibility tested by broth microdilution and disk diffusion methods in 3 laboratori

37 oScan dried panels with CLSI reference broth microdilution and disk diffusion methods on a collection

38 (QC) study was performed to establish broth microdilution and disk diffusion QC ranges for strains S

39 cal and Laboratory Standards Institute broth microdilution and disk diffusion reference methods.

40 Broth microdilution and disk diffusion susceptibility testing

41 Using data generated by standardized broth microdilution and disk diffusion test methods, the Antif

42 kacin and methicillin resistance using broth microdilution and disk diffusion testing.

43 Broth microdilution and drug disk diffusion assays demonstrate

44 ro correlations with in vivo outcome of both microdilution and Etest procedures may detect more-relev

45 C50/MIC9(0)s to </= 0.25/0.5 mug/ml by broth microdilution and Etest.

46 determined in triplicate via reference broth microdilution and interpreted according to CLSI guidelin

47 tablet assay to both reference M27-A2 broth microdilution and M44-A disk diffusion methods for testi

48 vancomycin susceptibility phenotype by broth microdilution and modified population analysis.

49 ncomycin MICs of 2 mug/ml by reference broth microdilution and screened for hVISA using PAP-AUC (21/1

50 oxycycline, lincomycin, and tylosin by broth microdilution and that to carbadox by agar dilution.

51 eement by all four dilution tests (two broth microdilution and two agar dilution) was achieved in onl

52 from enrolled patients were tested by broth microdilution and whole genome sequencing at a central l

53 n, Microscan broth microdilution, CLSI broth microdilution, and Etest).

54 cal and Laboratory Standards Institute broth microdilution, and selected isolates were typed by pulse

55 There are currently no FDA-approved broth microdilution antifungal susceptibility testing products

56 SA from the same geographic area using broth microdilution antimicrobial susceptibility testing, mult

57 MIC testing by both agar dilution and broth microdilution, as well as genospecies identification and

58 were tested using the CLSI M27-A2 method for microdilution, as well as the M-44A method and E-test me

59 Susceptibility was determined using a microdilution assay (Sensititre YeastOne; Trek Diagnosti

60 A broth microdilution assay using a 96-well plate was developed

61 A microdilution assay was used to determine antibacterial

62 n disk diffusion test and an oxacillin broth microdilution assay were examined.

63 tee for Clinical Laboratory Standards) broth microdilution assay, quality control (QC) MIC limits hav

64 e clinical isolates of the MAC using a broth microdilution assay.

65 -positive and Gram-negative bacteria using a microdilution assay.

66 sa and Staphylococcus aureus was assessed by microdilution assay.

67 cefoxitin disk diffusion and oxacillin broth microdilution assays categorized 100% and 61.3% of isola

68 stent with those obtained by classical broth microdilution assays for a range of antibiotics and bact

69 Cs were determined using Etest and two broth microdilution assays, MicroScan and Sensititre.

70 ibiotic-modifying activity were evaluated by microdilution assays.

71 e bacterium, Staphylococcus hyicus, in broth microdilution assays.

72 can be tested using broth macrodilution and microdilution assays.

73 ution MIC values compared to reference broth microdilution at +/-1 log(2) dilution were 88% and 94%,

74 g may be performed by broth macrodilution or microdilution at either pH, with NCCLS-recommended inter

75 e explored a novel, automated, at-will broth microdilution-based susceptibility testing platform.

76 r aztreonam-avibactam AST by reference broth microdilution (BMD) according to Clinical and Laboratory

77 l modal MIC concordance for testing by broth microdilution (BMD) and agar dilution was >96% for all t

78 Although both broth microdilution (BMD) and disk diffusion (DD) are listed b

79 ptible-only" interpretive criteria for broth microdilution (BMD) and disk diffusion (DD) testing of d

80 Broth microdilution (BMD) and disk diffusion methods have been

81 mycin and daptomycin MICs, measured by broth microdilution (BMD) and Etest, was prospectively assesse

82 the results were compared to reference broth microdilution (BMD) and to consensus results from three

83 st performed equivalently to oxacillin broth microdilution (BMD) and to oxacillin DD tests among S. a

84 brook 7H11 agar proportion (AP) assay, broth microdilution (BMD) assay, and mycobacterial growth indi

85 Illumina MiSeq WGS and broth microdilution (BMD) assays were performed on 90 bloodstr

86 However, broth microdilution (BMD) confirmatory testing at the Project

87 an panel compared to that of reference broth microdilution (BMD) during the testing of 64 strains enr

88 T-XN06 cards to those obtained by CLSI broth microdilution (BMD) for 255 isolates of Enterobacteriace

89 y Standards Institute (CLSI) reference broth microdilution (BMD) for 99 isolates of Pseudomonas aerug

90 derocol disk diffusion methods (DD) to broth microdilution (BMD) for AST of Gram-negative bacilli (GN

91 lution (CBDE) test compared to that of broth microdilution (BMD) for identifying colistin MICs.

92 s yield equivalent results to those of broth microdilution (BMD) for imipenem-relebactam susceptibili

93 thod that represents an alternative to broth microdilution (BMD) for performing antimicrobial suscept

94 ibility system was compared to that of broth microdilution (BMD) for the determination of MICs of var

95 nd voriconazole, using the CLSI M27-A3 broth microdilution (BMD) method (24-h incubation), in order t

96 y Standards Institute (CLSI) reference broth microdilution (BMD) method by testing 2 quality control

97 y Standards Institute (CLSI) reference broth microdilution (BMD) method by testing two quality contro

98 Studies using the EUCAST broth microdilution (BMD) method have defined wild-type (WT) M

99 The antifungal broth microdilution (BMD) method of the European Committee on

100 The antifungal broth microdilution (BMD) method of the European Committee on

101 test compared to determinations by the broth microdilution (BMD) method.

102 ry Standards (NCCLS)-approved standard broth microdilution (BMD) method.

103 ry Standards (NCCLS) approved standard broth microdilution (BMD) method.

104 and cefoxitin disk diffusion (DD) and broth microdilution (BMD) methods for the detection of mecA-me

105 gin, and micafungin, using CLSI M27-A3 broth microdilution (BMD) methods, in order to define wild-typ

106 robial Susceptibility Testing (EUCAST) broth microdilution (BMD) methods.

107 Laboratory Standards Institute (CLSI) broth microdilution (BMD) methods.

108 For all 131 isolates, the broth microdilution (BMD) MIC of at least one extended-spectru

109 mupirocin high-level resistance (HLR), broth microdilution (BMD) MICs of >or=512 microg/ml, and a 6-m

110 Laboratory Standards Institute (CLSI) broth microdilution (BMD) reference method for 134 staphylococ

111 Laboratory Standards Institute (CLSI) broth microdilution (BMD) reference method for 61 isolates of

112 cal and Laboratory Standards Institute broth microdilution (BMD) reference method for the detection o

113 cal and Laboratory Standards Institute broth microdilution (BMD) reference methods.

114 hat observed in a separate centralized broth microdilution (BMD) surveillance.

115 the MIC relative to the gold standard broth microdilution (BMD) test (MIC(50) and MIC(90) of 1 and 1

116 illin could be used as a surrogate for broth microdilution (BMD) testing of imipenem versus Enterococ

117 Disk diffusion and broth microdilution (BMD) were used to perform clindamycin (CL

118 occus aureus isolates using (i and ii) broth microdilution (BMD) with 50-mg/liter calcium medium supp

119 e evaluated by cefoxitin and oxacillin broth microdilution (BMD), disk diffusion (DD), and PBP2a immu

120 T), and doripenem (DOR) were tested by broth microdilution (BMD), Etest, and disk diffusion (DD), and

121 Broth microdilution (BMD), macrodilution (MD), and agar diluti

122 MIC agreement and error rates between broth microdilution (BMD), Vitek 2, and Etest against 48 clini

123 que are validated by the gold standard broth microdilution (BMD).

124 olistin resistant (CoR) when tested by broth microdilution (BMD).

125 oratories have the capacity to perform broth microdilution (BMD).

126 tive testing (Etest and CLSI reference broth microdilution [BMD] method) of stored isolates from 2006

127 e Colorimetric Antifungal plate to reference microdilution broth (NCCLS M27-A2 document) MICs of thre

128 boratory Standards (NCCLS) proposed standard microdilution broth method.

129 ) between each test system and the reference microdilution broth reference method for S. pneumoniae A

130 posaconazole were compared to reference 48-h microdilution broth visual MICs (CLSI [formerly NCCLS] M

131 nd Etest compared to that of reference broth microdilution by use of the revised breakpoints.

132 To address this issue, we utilized microdilution checkerboard assays to evaluate nine stilb

133 ich included disk diffusion, Microscan broth microdilution, Clinical and Laboratory Standards Institu

134 ing methods (disk diffusion, Microscan broth microdilution, CLSI broth microdilution, and Etest).

135 late Bank isolates for which reference broth microdilution colistin susceptibility results were avail

136 s, including susceptibility testing by broth microdilution, detection of Panton-Valentine leukocidin

137 A commercial broth microdilution device (Sensititre; Thermo Fisher Scientif

138 ormance of the HP D300 inkjet-assisted broth microdilution digital dispensing method (DDM), which was

139 es across four methods: agar dilution, broth microdilution, disk diffusion, and Etest.

140 We examined the correlation between the microdilution, E-test, and disk diffusion methods for po

141 ever, because visual interpretation of broth microdilution end points is subjective, it is more prone

142 compared using commercial and in-house broth microdilution, Etest, and common automated methods.

143 scribe the levels of agreement between broth microdilution, Etest, Vitek 2, Sensititre, and MicroScan

144 Isolates were susceptibility tested by broth microdilution, examined for inducible clindamycin resist

145 l'Etoile, France) compared to that of broth microdilution for 629 Enterobacterales and 163 Pseudomon

146 g agar-based methods a viable alternative to microdilution for posaconazole susceptibility testing.

147 testing (AST) methods were compared to broth microdilution for testing of Staphylococcus aureus and e

148 e compared Etest and disk diffusion to broth microdilution for the detection of fluoroquinolone resis

149 y Standards Institute (CLSI) reference broth microdilution, gradient diffusion (Etest), 23S rRNA gene

150 co-primary endpoints, were AUC/MIC by broth microdilution >=650 and AUC/MIC by Etest >=320.

151 All but one isolate tested by broth microdilution had MICs of < 1.0 microg/ml, while 96% of

152 Researchers employed microdilution high-volume sampling systems in conjunctio

153 rium tuberculosis (Mtb) isolates using broth microdilution in Middlebrook 7H9.

154 All isolates underwent testing by the broth microdilution (in duplicate) and agar dilution (in dupli

155 Only broth microdilution is recommended for polymyxin susceptibilit

156 These results indicate that broth microdilution may be a reliable method for fosfomycin su

157 stitute (formerly the NCCLS) reference broth microdilution method (BMD) by testing 2 quality control

158 The standard broth microdilution method (BMD) is demanding and requires exp

159 tes were determined by using the NCCLS broth microdilution method (BMD), and those isolates for which

160 ium apiospermum) by the CLSI reference broth microdilution method (M 38-A document).

161 linical Laboratory Standards reference broth microdilution method and Etest (amphotericin B).

162 cal and Laboratory Standards Institute broth microdilution method and the Etest GRD (glycopeptide res

163 oratory Standards Institute (CLSI) reference microdilution method by testing 2 quality control strain

164 nical Laboratory Standards (NCCLS) reference microdilution method by testing two quality control stra

165 However, a microdilution method demonstrated that CFX exhibited hig

166 tudy investigated the reproducibility of the microdilution method developed at the Center for Medical

167 droplets matched the MIC obtained from broth microdilution method for all strains.

168 Compared to the results of the broth microdilution method for detecting linezolid-nonsuscepti

169 od in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility

170 they were also tested by the reference broth microdilution method in parallel.

171 cal and Laboratory Standards Institute broth microdilution method incorporating cation-adjusted Muell

172 nce currently relies on a conventional broth microdilution method that requires a 16- to 20-h incubat

173 f the compounds was assessed using the broth microdilution method to determine the minimum inhibitory

174 ity rates determined with the standard broth microdilution method using cation-adjusted Mueller-Hinto

175 y Standards Institute (formerly NCCLS) broth microdilution method using Mueller-Hinton lysed horse bl

176 Similarly, concordance with the broth microdilution method was 40/43 isolates (93%) for both t

177 correlate well with both the E-test and the microdilution method, making agar-based methods a viable

178 sted for their susceptibilities by the broth microdilution method, they were tested for inducible cli

179 dilution steps) with the standardized broth microdilution method, validating the use of the Etest as

180 Using a reference broth microdilution method, we found that the serial isolates

181 ted States, all tested by the CLSI reference microdilution method.

182 nce Program, all tested by a reference broth microdilution method.

183 2) dilutions compared to the reference broth microdilution method.

184 B, C, F, and G were tested by the CLSI broth microdilution method.

185 s were determined using a standardized broth microdilution method.

186 ards Institute (CLSI) M27-A2 reference broth microdilution method.

187 testing is an attractive alternative to the microdilution method.

188 diameters was better for the E-test than the microdilution method.

189 solates of Candida spp. using the CLSI broth microdilution method.

190 ttee for Clinical Laboratory Standards broth microdilution method.

191 fluconazole as determined by the NCCLS broth microdilution method.

192 da species by both the CLSI and EUCAST broth microdilution methodologies.

193 g unit of the standard inoculum, using broth microdilution methodology with ceftazidime, cefotaxime,

194 boratory Standards Institute reference broth microdilution methodology.

195 ycline compounds by disk diffusion and broth microdilution methods according to CLSI guidelines.

196 activity was evaluated by agar diffusion and microdilution methods and antioxidant activity by DPPH a

197 Therefore, reference broth microdilution methods and MIC ranges for quality control

198 CLSI reference broth microdilution methods and species-specific interpretive

199 d by CLSI disk diffusion and reference broth microdilution methods in the central reference laborator

200 by NCCLS disk diffusion and reference broth microdilution methods in the central reference laborator

201 rog/ml, the agreement between the E-test and microdilution methods was 87.8% at 24 h and 93.0% at 48

202 well dilutions) between the Etest and broth microdilution methods was 94%.

203 Agar dilution and broth microdilution methods were evaluated.

204 (Etest, disk diffusion, and Sensititre broth microdilution methods) for testing of minocycline, tigec

205 Program, 1997 to 2004) were tested by broth microdilution methods, and 399 randomly selected strains

206 and micafungin were determined by CLSI broth microdilution methods.

207 cal and Laboratory Standards Institute broth microdilution methods.

208 erformed by the CLSI M27-A3 and M38-A2 broth microdilution methods.

209 n (102 isolates) as determined by CLSI broth microdilution methods.

210 MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coeffic

211 titute (CLSI) M23 tier 2 study design, broth microdilution MIC and disk diffusion quality control (QC

212 CLSI broth microdilution MIC data gathered in five independent labo

213 ical isolates using the CLSI reference broth microdilution MIC method demonstrated a tendency toward

214 erculosis drugs, with a convenient 7H9 broth microdilution MIC method suitable for use in resource-li

215 rug-resistant tuberculosis using a 7H9 broth microdilution MIC method.

216 ratio of 400-600 mg*hour/L (assuming a broth microdilution MIC of 1 mg/L) to achieve clinical efficac

217 Broth microdilution MIC QC ranges spanned 3 to 4 doubling dilu

218 In a separate study, broth microdilution MIC quality control ranges for zoliflodaci

219 azole and voriconazole compared to the broth microdilution MIC reference method.

220 Reference broth microdilution MIC results for 12,796 strains of Candida

221 Reference broth microdilution MIC results for 13,338 strains of Candida

222 Broth microdilution MIC results were compared by scattergram a

223 tory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mec

224 imicrobial susceptibility results from broth microdilution MIC testing of 993 Staphylococcus lugdunen

225 itute (CLSI, formerly NCCLS) reference broth microdilution MIC testing was performed on all clinicall

226 a subculture of the isolate was tested by a microdilution MIC using YeastOne (TREK Diagnostics Syste

227 ges were approved by the CLSI in 2017 (broth microdilution MIC) and 2019 (disk diffusion).

228 For the 7H9 broth microdilution MIC, a 3-dilution QC range (0.015 to 0.06

229 s and 0.015 to 0.06 mug/ml for the 7H9 broth microdilution MIC.

230 were determined to be PB resistant by broth microdilution (MIC > 2 mug/ml), including all 7 JMI isol

231 The E-test and microdilution MICs show good concordance and interpretat

232 test demonstrated 82.6% agreement with broth microdilution MICs, a very major error rate of 2.2%, and

233 egorical agreement (CA) with reference broth microdilution MICs.

234 ed positive for amikacin resistance by broth microdilution or disk diffusion testing were investigate

235 the performance of a new colorimetric broth microdilution panel (SensiQuattro Candida EU) for antifu

236 A commercially prepared, dried colorimetric microdilution panel (Sensititre YeastOne Trek Diagnostic

237 A commercially prepared dried colorimetric microdilution panel (Sensititre YeastOne, TREK Diagnosti

238 and 2 microg/ml) in a single well of a broth microdilution panel to predict the presence of inducible

239 We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, a

240 ach isolate was tested by a frozen reference microdilution panel, the MicroScan ESbetaL plus confirma

241 For each isolate, a total of 30 microdilution panels from three different lots were test

242 Results were compared to frozen microdilution panels prepared according to NCCLS specifi

243 etry (MALDI-TOF MS) identification and broth microdilution phenotypic susceptibility testing on clini

244 ood culture broths by using Sensititre broth microdilution plates compared to testing with isolated c

245 IC test strip and the EUCAST reference broth microdilution protocol.

246 , Sparks, MD) radiometric method and a broth microdilution reference method.

247 tococci) with the Phoenix system and a broth microdilution reference method.

248 ng investigational test cards and by a broth microdilution reference method.

249 parison to the results obtained with a broth microdilution reference standard.

250 Standardized broth microdilution reference tests were compared to the zone

251 l and 512 mug/ml for agar dilution and broth microdilution, respectively.

252 ent between the agar-based methods and broth microdilution results ranged from 93 to 98%, with <1% ve

253 nt between the 24-h and reference 48-h broth microdilution results ranged from 93.8% (all Candida spe

254 nt between the 24-h and reference 48-h broth microdilution results ranged from 97.1% (C. parapsilosis

255 In comparison with the standard broth microdilution results, very major rates were low ( appro

256 A broth microdilution scheme allowed direct comparison of the MI

257 Isolates tested by the broth microdilution showed high levels of resistance; suscepti

258 an 8 h, was compared with the standard broth microdilution susceptibility assay (Clinical and Laborat

259 ach inoculated onto specially prepared broth microdilution susceptibility panels containing vancomyci

260 oratory study was conducted to compare broth microdilution susceptibility results using ambient air (

261 rlaboratory reproducibility with caspofungin microdilution susceptibility testing against panels comp

262 The telavancin broth microdilution susceptibility testing method was revised,

263 e performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical i

264 e performed Etest, disk diffusion, and broth microdilution susceptibility testing of posaconazole aga

265 entation is the recommended medium for broth microdilution susceptibility tests of Brucella abortus,

266 For broth microdilution susceptibility tests of Francisella tulare

267 Institute (CLSI)-recommended method of broth microdilution, susceptibility testing of 170 isolates of

268 Standardized broth microdilution techniques can be used to distinguish FKS

269 By broth microdilution techniques, we determined the MIC values o

270 Clinical Laboratory Standards (NCCLS) broth microdilution test at center 1 (C1).

271 is study has demonstrated that a single-well microdilution test incorporating erythromycin and clinda

272 be resistant to oxacillin by reference broth microdilution testing (MIC, 8 microg/ml), one isolate wa

273 ted to a central reference monitor for broth microdilution testing by Clinical and Laboratory Standar

274 I Laboratories, North Liberty, IA) for broth microdilution testing by reference methods.

275 While standardized microdilution testing methodologies and quality control

276 iology laboratories to perform at-will broth microdilution testing of antimicrobials and to address a

277 lity control ranges were developed for broth microdilution testing of Campylobacter jejuni ATCC 33560

278 owed unsatisfactory reproducibility of broth microdilution testing of ceftriaxone with N. cyriacigeor

279 six laboratories performed repetitive broth microdilution testing on single strains of Nocardia bras

280 ld serial dilution series required for broth microdilution testing.

281 Macrotiter or microdilution tests were performed according to the NCCL

282 orption ionization-time of flight, and broth microdilution tests were repeated to confirm the CRE phe

283 lity control (QC) ranges for reference broth microdilution tests.

284 h method were compared with those from broth microdilution (the reference method), and agreement was

285 For Etest compared to the reference broth microdilution, the essential agreement was 100% for both

286 from 94.9% (broth macrodilution versus broth microdilution) to 98.6% (agar dilution versus broth micr

287 isolates were tested simultaneously by broth microdilution using freshly prepared Mueller-Hinton brot

288 Broth microdilution using standard cation-adjusted Mueller-Hin

289 f Mycobacterium avium complex (MAC) by broth microdilution using two different media (cation-adjusted

290 evaluated the performance of the 24-h broth microdilution voriconazole MIC by obtaining MICs for 2,1

291 within two dilutions) between the E-test and microdilution was 64.8% at 24 h and 82.6% at 48 h.

292 agreement) of AXDX MICs with reference broth microdilution was 98.0% (96/98).

293 SensiQuattro panel with the reference broth microdilution was slightly higher for C. albicans (87%)

294 of the agar-based methods and those of broth microdilution were 96 to 98%, with no very major errors.

295 MICs obtained by broth microdilution were different than MICs by any other meth

296 tes determined to be PB susceptible by broth microdilution were NP test negative.

297 analyzed using both agar dilution and broth microdilution with a resulting high essential agreement

298 t (MDR) Gram-negative bacilli (GNB) by broth microdilution with polysorbate 80 (BMD-T), broth macrodi

299 ted the most agreement with those from broth microdilution, with 95.6% agreement based on the MIC and

300 allel using BMD-T, TDS, agar dilution, broth microdilution without polysorbate 80 (BMD), and the TREK