ライフサイエンスコーパス: micropipette

1 chemotactic efficiency as they approach the micropipette.

2 d tissues where cells can be targeted with a micropipette.

3 dual-beam lasers and the other was held by a micropipette.

4 0.2 M) microinjections (50 nl) from a glass micropipette.

5 ymersomes using either optical tweezers or a micropipette.

6 ssue, and individually loaded onto a suction micropipette.

7 cal acid injection via a cell-attached glass micropipette.

8 gative pressure applied to the membrane by a micropipette.

9 a single red blood cell is aspirated into a micropipette.

10 e calculated from red cell velocities in the micropipette.

11 ectroporates a single cell at the tip of the micropipette.

12 d controllably deformed by aspiration into a micropipette.

13 that was constrained to the vicinity of the micropipette.

14 ylcholine injection into the compact AVN via micropipette.

15 ations of a coarse-grained network held in a micropipette.

16 ting antibodies and bending stiffness of the micropipette.

17 particles immobilized and manipulated with a micropipette.

18 aining 0.05% Triton X-100 on it from a third micropipette.

19 simulations of erythrocyte deformation in a micropipette.

20 Alexa Fluor 488 was injected via an adjacent micropipette.

21 was measured concurrently with a servo-null micropipette.

22 ement of a microsphere by using a fine glass micropipette.

23 raocular pressure was directly assessed with micropipettes.

24 ction of these rare marrow cells using glass micropipettes.

25 of cardiac surgery and cannulated with glass micropipettes.

26 s during cardiac surgery and cannulated with micropipettes.

27 roteins directly applied to growth cones via micropipettes.

28 Neural recordings were made with glass micropipettes.

29 endent electrodes fabricated on pulled glass micropipettes.

30 by glutamate which was pressure-ejected from micropipettes.

31 ked by GABA, which was pressure-ejected from micropipettes.

32 attached patch recordings using quartz-glass micropipettes.

33 ng the local membrane elasticity measured by micropipettes.

34 responses to glutamate, applied locally via micropipettes.

35 endent vasoactive agents were delivered from micropipettes (0.5 or 1 second pulse) onto an arteriole

36 rom 1.8 to 12.6 mm in inner diameter (ID), 6 micropipettes (0.7- or 1.1-mm ID filled with (64)Cu at 5

37 lutamate microelectrodes equipped with glass micropipettes 50 microm from the recording surfaces were

38 12)-10(-4) mol l(-1)) was applied dose-wise (micropipette, 60 seconds).

39 After withdrawal of the micropipette, a second penetration led to a similar leve

40 When released from a micropipette, acetylcholine (ACh) triggered vasodilatati

41 ll wound is induced in an intact layer using micropipette action and responses in neighboring cells a

42 combinant PECAM as assessed in a single-cell micropipette adhesion assay able to measure the biophysi

43 he peptide:MHC monomer-based two-dimensional micropipette adhesion frequency assay confirmed that NFM

44 ested this assumption by using data from the micropipette adhesion frequency assay that generates seq

45 ractions between T cells and APCs, we used a micropipette adhesion frequency assay to measure the adh

46 The micropipette adhesion frequency assay was used to obtain

47 By using a micropipette adhesion frequency assay, we show that this

48 Utilizing the highly sensitive micropipette adhesion frequency assay, which allows one

49 plasmon resonance and in two dimensions by a micropipette adhesion frequency assay.

50 Using the two-dimensional micropipette adhesion-frequency assay, we show that TRM

51 , 100 microm AP-5, or 50 microm CNQX) from a micropipette adjacent to the recording electrode signifi

52 pricking the rear of a slug with an unfilled micropipette, also cause a more limited nuclear transloc

53 moved toward a chemoattractant emitted by a micropipette, although persistence was lower than that o

54 Using two micropipettes, an avidin-coated vesicle was presented to

55 ions, an exponential gradient emitted from a micropipette and a shallow, linear gradient in a Dunn ch

56 ed detection scheme utilizing a borosilicate micropipette and an assay of complementary gamma-peptide

57 cted by iontophoresis through a single glass micropipette and detected by immunohistochemistry.

58 battery-powered instrumentation (electronic micropipette and potentiostats) is commercially availabl

59 er-oleic acid (W-O) mixtures with the aid of micropipette and shaking.

60 n the impedance of the interface between the micropipette and the cell interior, which limits how sma

61 ce using the adhesion frequency assay with a micropipette and the thermal fluctuation and force-clamp

62 The IFP was measured with micropipettes and a servo-null system.

63 carotid arteries were mounted between glass micropipettes and kept fully vasodilated.

64 mpedance, they can be made much smaller than micropipettes and microelectrodes.

65 vessel is cannulated at both ends with glass micropipettes and the fluid filtration rate across the v

66 pler is employed, it is not necessary to use micropipettes and, nevertheless, precise measurements ar

67 myocytes were introduced into the chamber by micropipetting and subsequently capped with a layer of m

68 ds for allergen application (metered spray & micropipette) and NAC protocols (NAC with single or mult

69 the bottom of the flask was collected with a micropipette, and Cu and Ni were determined by graphite

70 second study, red cells are aspirated into a micropipette, and immunofluorescent maps of the surface

71 y used, including magnetic, angular-optical, micropipette, and magneto-optical tweezers.

72 ophoretically or pressure ejected from glass micropipettes, and 7 days later the animals were euthani

73 m o.d.) were isolated, cannulated with glass micropipettes, and pressurized to 85 mmHg.

74 rom OD) were isolated, cannulated with glass micropipettes, and pressurized.

75 olated from male rats, cannulated with glass micropipettes, and pressurized.

76 Focal micropipette application of either ACh, to stimulate end

77 e, the micropipette force probe, that uses a micropipette as a flexible cantilever that can aspirate

78 This perspective highlights the relevance of micropipette-aspirated single-particle tracking-used to

79 al properties of lipid bilayers by combining micropipette aspiration (MA) with theoretical modeling.

80 Here, we use micropipette aspiration (MPA) to directly characterize t

81 also caused the projections induced both by micropipette aspiration and by lysophosphatidylcholine t

82 coupling can be obtained by a combination of micropipette aspiration and fluctuation spectroscopy mea

83 In this work, we utilize micropipette aspiration and fluorescence imaging to exam

84 First, we simulated the micropipette aspiration and quantified the cytoskeletal

85 tion, we sorted spheroids using an automated micropipette aspiration and release system and monitored

86 Here, we develop micropipette aspiration and whole-cell patch-clamp (MAPA

87 The dynamics of human neutrophils during micropipette aspiration are frequently analyzed by appro

88 hrough micropores and nuclear flexibility in micropipette aspiration both appear limited by lamin-A:B

89 model and its simplified version to analyze micropipette aspiration computationally and analytically

90 Micropipette aspiration demonstrated decreased cortical

91 Furthermore, micropipette aspiration experiments revealed increased e

92 e aging, we use a combination of established micropipette aspiration experiments together with two op

93 ed from comparable rupture times in separate micropipette aspiration experiments.

94 With five decades of sustained application, micropipette aspiration has enabled a wide range of biom

95 itatively by our atomic force microscopy and micropipette aspiration measurements of iRBCs.

96 ment with previously reported values and our micropipette aspiration measurements.

97 We used the micropipette aspiration method, by which the changes of

98 Micropipette aspiration of cells shows myosin-II accumul

99 We utilized micropipette aspiration of giant unilamellar vesicles co

100 We have utilized micropipette aspiration of giant unilamellar vesicles to

101 rotein-lamin B dynamics were visualized in a micropipette aspiration of isolated nuclei, and both wer

102 Furthermore, studies using micropipette aspiration of single vesicles reveal that c

103 We develop a model to analyze the micropipette aspiration of these compressible gas vesicl

104 fluid motion around colonies immobilized by micropipette aspiration reveals flow fields with very la

105 Our results from micropipette aspiration suggest that cholesterol and erg

106 existence of fluid and gel-like domains, the micropipette aspiration technique allowed measurements o

107 In this study, using the micropipette aspiration technique and fluorescence micro

108 In this paper, we use the micropipette aspiration technique to characterize the el

109 The micropipette aspiration technique was used to determine

110 a custom-designed microscope chamber for the micropipette aspiration technique.

111 tension experiments at 37 degrees C with the micropipette aspiration technique.

112 uble tethers from endothelial cells with the micropipette aspiration technique.

113 ong the three layers were evaluated with the micropipette aspiration technique.

114 to investigate by alternative methods, i.e., micropipette aspiration technique.

115 , we examined EC surface protrusion with the micropipette aspiration technique.

116 s or overexpresses various myosin I's, using micropipette aspiration techniques.

117 We used micropipette aspiration to directly measure the area com

118 oplasmic membranes by applying the method of micropipette aspiration to Escherichia coli spheroplasts

119 Employing micropipette aspiration to mimic squeezing through narro

120 We employ micropipette aspiration to show that anisotropic tension

121 Micropipette aspiration was used to measure the pressure

122 Micropipette aspiration was used to test mechanical stre

123 on analysis, vesicle electrodeformation, and micropipette aspiration were used to assess the bending

124 istent with both dual pipette aspiration and micropipette aspiration, a problem not successfully tack

125 methods, such as atomic force microscopy and micropipette aspiration, by several orders of magnitude.

126 Local measurements by micropipette aspiration, however, have reported only an

127 Using micropipette aspiration, we show that the lamina in HGPS

128 han the value reported for intact cells from micropipette aspiration.

129 in Monte Carlo simulations of whole cells in micropipette aspiration.

130 ate their effect on membrane mechanics using micropipette aspiration.

131 quantify cell-substrate adhesion force using micropipette aspiration.

132 , thermal degradation, and applied stress by micropipette aspiration.

133 Here, with the micropipette-aspiration technique, we show that tethers

134 e have determined this relationship with the micropipette-aspiration technique.

135 -cell receptor (TCR) signalling reporter and micropipette assay to quantify naive precursors and expa

136 etermine molecular density (expression), and micropipette assays are used to find the probability of

137 A five barrel micropipette assembly was used for extracellular recordi

138 Glass micropipettes, atomic force microscope tips and nanoneed

139 jected into spinal segments T12-L3 through a micropipette attached to a Hamilton microliter syringe.

140 atory include traditional transfer pipettes, micropipettes based on air displacement, and motorized d

141 Using micropipette-based force measurements and epigenetic dru

142 Micropipette-based measurements of cadherin-mediated, ce

143 liter droplets of a second reagent through a micropipette by means of a pressure-driven droplet injec

144 ese agents and K+ (150 mM) were applied from micropipettes by brief (1 s) microperfusion pulses.

145 he cell interior, which limits how small the micropipette can be.

146 define the initial contact time (+/-50 ms), micropipette cell manipulation was used to bring individ

147 single cells were retrieved within the same micropipette column, with each cell encapsulated in a fl

148 ed for up to four single cells retrieved per micropipette column.

149 pressure measurements were obtained using a micropipette connected to a servonull device and positio

150 s the viability of the oil-immersed scanning micropipette contact method and opens up the avenue to m

151 Quantitative scanning micropipette contact method measurements are subject to

152 potentials during the oil-immersed scanning micropipette contact method measurements.

153 The oil-immersed scanning micropipette contact method significantly increases the

154 turized electrochemical cell of the scanning micropipette contact method was found to leak Ag(+) into

155 the development of an oil-immersed scanning micropipette contact method, a variant of the scanning m

156 75-T73 alloy using the oil-immersed scanning micropipette contact method, the cathodic current was in

157 te contact method, a variant of the scanning micropipette contact method, where a thin layer of oil w

158 lly challenging in the conventional scanning micropipette contact method.

159 currents inherently measured in the scanning micropipette contact method.

160 The micropipette contained 130 mM CsCl and 1 microM QX-314.

161 emoattractant, and orient and crawl toward a micropipette containing chemoattractant.

162 led by micromanipulating bound microbeads or micropipettes, cytoskeletal filaments reoriented, nuclei

163 in chains to patterned photobleaching of the micropipette-deformed network.

164 sults for single actin filaments, imaging of micropipette-deformed red cell ghosts has allowed an ass

165 les with the cell adhesion area (for a given micropipette diameter and loading rate), which defines a

166 Since the micropipette diameter and the aspiration pressure are ou

167 usoidally displacing the cupula with a glass micropipette driven with a piezoelectric device while re

168 aspirating large unilamellar vesicles into a micropipette electrode, we are able to simultaneously mo

169 ement of solute concentrations by indwelling micropipette electrodes and the collection of perfusate

170 When micropipette electrodes containing NMDA receptor antagon

171 As determined in micropipette experiments, microvilli deform like an elas

172 atively comparable to experimental data from micropipette experiments.

173 information on cortical activity by means of micropipette experiments.

174 Using glass micropipettes, extracellular isolation of 37 interpolari

175 r in combination with estrogen through glass micropipettes fastened to the electrodes.

176 Electrical stimulation through a micropipette filled with DNA or other macromolecules ele

177 The patch clamp technique, in which a glass micropipette filled with electrolyte is inserted into a

178 s assessed by voltage clamp of oocytes using micropipettes filled with 2 M NaCl.

179 Here we describe a new technique, the micropipette force probe, that uses a micropipette as a

180 Using a micropipette force sensor in an oscillating mode, we mea

181 directly measure their bending moduli using micropipette force sensors, and quantify propulsion and

182 We present novel experiments in which two micropipette-held somatic cells of Volvox carteri, with

183 distorted cell, are visually demonstrated in micropipette-imposed deformation.

184 tactic accuracy and speed as they approach a micropipette in a manner that is dependent on the increa

185 Single vesicles were manipulated by micropipette in solutions of fluorescently labeled avidi

186 teries were isolated, removed and mounted on micropipettes in a sealed chamber.

187 cles of wild-type mice following perivenular micropipette injections of a control (LSIGRL) or PAR2-ac

188 Whole-cell K(+) currents recorded with a micropipette inserted into the cell soma were Ba(2+)-sen

189 mber is used, making it possible to insert a micropipette inside this chamber to hold a second bead b

190 ndard tool for IC recording, the patch-clamp micropipette is applied widely, yet remains limited in t

191 ansient current exceeds a set threshold, the micropipette is automatically halted.

192 crospheres inside the lymphatic lumen with a micropipette is blocked by the lymphatic endothelium.

193 The micropipette is positioned perpendicular to the surface

194 cities of red cells when the pressure in the micropipettes is balanced.

195 f the initial lymphatic endothelium with the micropipette leads to rapid aspiration of intralymphatic

196 re used: Group I received a unilateral glass micropipette lesion into the vermal/paravermal region of

197 Second, a micropipette-loaded with magnetic nanoparticles to which

198 rigid micropores and in passive pulling into micropipettes, local compaction of chromatin is observed

199 zone), aggregometry (discrete interactions), micropipette manipulation (tether visualization), and ro

200 Micropipette manipulation measurements quantified the pr

201 Using micropipette manipulation of giant unilamellar vesicles,

202 n rat cremaster muscle was investigated with micropipette manipulation techniques.

203 Micropipette manipulation was used to expose a single li

204 o different methods, electron microscopy and micropipette manipulation, we have obtained two comparab

205 ptical-lever detection module with automated micropipette manipulation.

206 ilt, horizontal atomic force microscope with micropipette manipulation.

207 neutrophils or other amoeboid cells inside a micropipette, measurement of velocity versus counterpres

208 ly consistent with experimental results from micropipette measurements.

209 Binding to IgG-coated RBCs, measured using a micropipette method, indicated a 50-fold increase in eff

210 By using a micropipette method, we measured the kinetics of human F

211 vant presentation-using a recently developed micropipette method.

212 h juxtacellular Neurobiotin ejection, linked micropipette-microwire recording, and antidromic and ort

213 tone or benzophenone) are delivered into the micropipette needle (tip size ~ 15 um) through a fused s

214 The micropipette needle is produced by combining a pulled gl

215 We developed a new technique using a micropipette needle, in which Paterno-Buchi (PB) reactio

216 red microinjection system coupled to a glass micropipette needle.

217 ld an automated liquid handler that controls micropipetting of liquids in 3D space at speeds and posi

218 Acetylcholine delivery from a micropipette onto a feed artery evokes hyperpolarisation

219 muli were produced by ejecting buffer from a micropipette onto the cell surface with a pneumatic pico

220 oaches, our solution eliminates the need for micropipettes or electrical equipment, making it user-fr

221 DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by compu

222 ment is a compelling alternative to use of a micropipette, or to magnetic, electrical, and optical tr

223 robotic automation is difficult, however, as micropipette penetration induces tissue deformation, mov

224 e consistent with those determined using the micropipette perfusion and microdiffusion chamber techni

225 he use of the microdiffusion chamber and the micropipette perfusion technique, both of which have bee

226 chidonic acid and skin-permeable peroxide by micropipette perfusion to unwounded zebrafish tail fins.

227 al cortical injection of adenosine through a micropipette produced dose-dependent transient increases

228 The experiment was an extension of existing micropipette protocols.

229 to overcome these limitations by combining a micropipette (pulled glass capillary) based sample colle

230 Micropipette recording with juxtacellular Neurobiotin ej

231 ist-containing solution through a fine glass micropipette resulted in a spatially restricted increase

232 Furthermore, we used micropipette sampling to study intercellular heterogenei

233 l protocol designed to approximately mimic a micropipette setting, we show that asymmetric incorporat

234 The integrated micropipette setup facilitates the easy manipulation and

235 Ideally, the micropipette should be as small as possible to increase

236 Aspiration of a cell and its nucleus into a micropipette shows that chromatin aligns and stretches p

237 Using a micropipette single cell adhesion assay able to measure

238 area expansion that is independent of either micropipette size or aspiration pressure.

239 r vesicles, each of which was aspirated in a micropipette so that we could monitor the tension of the

240 or Gi, a trimeric G protein) responded to a micropipette source of attractant by localizing RhoA act

241 External calibration curves were prepared by micropipetting standards with internal standard (IS) on

242 In contrast, Ag conjugated to the tip of a micropipette stimulates local, repetitive Ca(2+) puffs a

243 asuring piconewton-scale forces that employs micropipette suction is presented here.

244 With micropipette suction, we applied pulling forces to human

245 mitotic newt chromosomes was studied, using micropipette surgery and manipulation, for elongations u

246 n electrophysiologic approach-the servo-null micropipette system (SNMS)-for measuring hydrostatic pre

247 Arterioles were cannulated with a micropipette system and luminally pressurized.

248 electrophysiologic approach (the servo-null micropipette system, SNMS) that had been adapted for con

249 nes was demonstrated by using the aspiration micropipette technique on red blood cells.

250 at the molecular level, we have developed a micropipette technique to quantify the redistribution of

251 Using a new micropipette technique, spherical, glassified protein mi

252 al cells from endothelial monolayers using a micropipette technique.

253 Myocytes were attached to micropipettes that extended from a force transducer and

254 ogenization, Triton-skinned, and attached to micropipettes that projected from a force transducer and

255 cell is caught and held between two suction micropipettes the surface membrane is destroyed by brief

256 he passive aspiration of a neutrophil into a micropipette, the active extension of a pseudopod by a n

257 WO tears were collected from 48 patients by micropipette, the WO sample after instillation of 10 muL

258 cause they are mounted on conventional glass micropipettes, the endoscopes readily fit standard instr

259 Evaporation is prevented by positioning the micropipette through a tiny hole in a cover glass, seale

260 sor is embedded in the internal surface of a micropipette tip as the aptasensor substrate for the lab

261 tended distance between the Ag/AgCl wire and micropipette tip droplet eliminated the Ag(+) contaminat

262 was applied on the smaller end of a 1000 muL micropipette tip made of polypropylene.

263 s in a lipid bilayer formed at a patch-clamp micropipette tip under a buffer solution.

264 The micropipette tip was visualized in the anterior chamber.

265 , microiontophoresis of acetylcholine (1 mum micropipette tip, 1 muA, 500 ms) initiated dilatation th

266 the color change of the solution inside the micropipette tip.

267 Local delivery (1 microm micropipette tip; 500-2000 ms pulse) of acetylcholine (A

268 Multi-barreled glass-micropipettes (tip size 20-40 microm) were used to make

269 lls were biotinylated and manipulated with a micropipette to form an adhesive contact with a glass mi

270 affect neural activity in the AC, we used a micropipette to infuse salicylate (20 mul, 2.8 mM) into

271 noliters of whole blood, and only requires a micropipette to operate.

272 ure ejection or iontophoresis of ions from a micropipette to quantify diffusion characteristics of ne

273 e applied potential during the approach of a micropipette to the substrate generates a transient curr

274 utrophils and immobilized ICAM-1 while using micropipettes to control the force of contact between th

275 cular mechanoreceptors, TTX was applied with micropipettes to proximal segments of feed arteries, the

276 s, and the crawling of a neutrophil inside a micropipette toward a chemoattractant against a varying

277 ncturing of transporting sieve elements with micropipettes triggered the rapid (<1 min) development o

278 Novel curves were identified by micropipetting U-dHRM reactions and Sanger sequencing am

279 lectrolyte that was suspended into two glass micropipettes using a conductive hydrogel.

280 Au nanoparticles (NPs) were coated on micropipettes using aminosilane linkers; and these micro

281 sensitivity to acid solutions, using the two-micropipette voltage clamp in Xenopus oocytes.

282 Diffusion-controlled micropipette voltammetry revealed that 2,6-diphenylpyrid

283 stress of fluid aspirated into a large-bore micropipette was then used to forcibly peel myotubes.

284 closed downstream, pressure (applied via the micropipette) was raised in a series of steps of 10 mmHg

285 By using a micropipette, we measured the conformational regulation

286 Fine-tipped (5 microm in diameter) micropipettes were advanced across the cornea with a pie

287 Stimulating micropipettes were inserted stereotaxically into the lat

288 For in vivo studies, micropipettes were used for recording and injecting vehi

289 ipettes using aminosilane linkers; and these micropipettes were used for stimulating and inhibiting t

290 Micropipettes were used to bring neutrophils into contac

291 urrent-clamp recordings with high-resistance micropipettes were used to characterize electrophysiolog

292 racellular recording techniques with 'sharp' micropipettes were used to evoke action potentials (APs)

293 Multibarrel glass micropipettes were used to record the activity of NST ne

294 th of the network projection pulled into the micropipette, where the network is strongly sheared in a

295 h the efficient continuous liquid feeding of micropipettes while allowing scalability to 1- and 2D pr

296 bility by whole cell patch using a multiplex micropipette with a common outlet to change artificial C

297 The DMB is a pulled glass micropipette with a fine tip that contains a microscopic

298 llisecond scale involves placing a recording micropipette with a membrane patch in front of a double-

299 The vessels were perfused via micropipettes with Ringer solutions containing bovine se