ライフサイエンスコーパス: midzone

1 stricting centromere movement to the spindle midzone.

2 ity with higher turnover rates nearer to the midzone.

3 e enrichment of Cin8 and Kip1 at the spindle midzone.

4 ty of the microtubules polymerize toward the midzone.

5 erlies the explosive assembly of the spindle midzone.

6 spindlin to accumulate stably at the spindle midzone.

7 from the chromosomes to the anaphase spindle midzone.

8 of multiple furrows that ingress toward the midzone.

9 uction of microtubule density in the spindle midzone.

10 y in anaphase that is centred at the spindle midzone.

11 ke the form of articular chondrocytes in the midzone.

12 ithin the cleavage furrow around the spindle midzone.

13 hed the localization of Cep55 to the spindle midzone.

14 somes, spindle microtubules, and the spindle midzone.

15 t imparts matrix-like characteristics to the midzone.

16 to the mitotic kinetochores and the spindle midzone.

17 tiparallel microtubule overlap at the cell's midzone.

18 localized to chromosomes and to the spindle midzone.

19 allel near the poles and antiparallel in the midzone.

20 ho-interacting kinase citron to the anaphase midzone.

21 se of restoring MT engagement in the spindle midzone.

22 tely a reduction of ZEN-4-GFP at the spindle midzone.

23 h targets and maintains ZEN-4 at the spindle midzone.

24 in metaphase and then with anaphase spindle midzone.

25 opposing the force generated by the spindle midzone.

26 ndle by promoting instability of the spindle midzone.

27 e, it is localized indirectly to the spindle midzone.

28 nd promoting transport of the complex to the midzone.

29 actors, Aurora B and Kif4, act as they do in midzones.

30 m for investigating plus-end organization in midzones.

31 the centromeres and relocates to the spindle midzone [7].

32 The spindle midzone, a conspicuous network of antiparallel interdigi

33 ision depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules

34 e ase1p-mediated recruitment of klp9p to the midzone accelerates pole separation, as suggested by com

35 provide cells with a modular system to tune midzone activity and control elongation rates.

36 the anti-parallel microtubule overlap of the midzone, after which the MKlp2-CPC complex moves in a no

37 Midzones, also called central spindles, are an array of

38 e equator by centralspindlin, Ect2's spindle midzone anchor complex, and is temporally coordinated wi

39 ubule-bundling protein that localizes to the midzone and also to microtubule bundles in the cytoplasm

40 DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule-depolymeri

41 onset, when it redistributed to the spindle midzone and became concentrated at the equator along mid

42 both a very slow rate of turnover within the midzone and limited lateral diffusion along spindle MTs.

43 cytokinesis, apparently because the spindle midzone and midbody microtublues were absent during late

44 ow completely regressed, even though spindle midzone and midbody microtubules appeared normal.

45 ment of cell division factors to the spindle midzone and midbody remain unclear.

46 tion is dispensable for the formation of the midzone and midbody, it promotes contractility and is re

47 omplex, a conserved component of the spindle midzone and midbody.

48 iorientation and organization of the spindle midzone and midbody.

49 In general, MAPs and motors stabilize the midzone and motors produce sliding.

50 pathway depends on a well-organized spindle midzone and PLK1, while the other depends on Aurora B ac

51 e that CPCs function to organize the spindle midzone and potentially switch motors between force gene

52 lization of MKLP1, PRC1, and Aurora B to the midzone and prevents the formation of a midbody matrix.

53 e intercellular canal containing the spindle midzone and resealing of the daughter cells.

54 actin cables in a gap between the monopolar midzone and the furrow-like cortex, suggesting a communi

55 rometaphase and metaphase and to the spindle midzone and the midbody during anaphase and cytokinesis.

56 was also concentrated on MTs in the spindle midzone and the phragmoplast.

57 strate that dynamin localizes to the spindle midzone and the subsequent intercellular bridge in mamma

58 entional dynamin is recruited to the spindle midzone and the subsequent intercellular bridge, where i

59 o centrosomes, kinetochores, and the spindle midzone and what the specific tasks of Plk1 at these dis

60 t a specific time during cytokinesis, making midzones and mitotic spindles differ in their dynamics a

61 bidopsis, ATK5, localizes to mitotic spindle midzones and regions rich in growing plus-ends within ph

62 bundle microtubules), construct the spindle midzone, and complete cytokinesis.

63 us and ectopic I-2 localized to the spindle, midzone, and midbody of mitotic human epithelial ARPE-19

64 uted to the kinetochore, centrosome, spindle midzone, and midbody, all of which are known to play cri

65 when SPD-1 is depleted, there is no visible midzone, and only this furrow localization remains.

66 ein associated with centromeres, the spindle midzone, and poles during mitosis and the cleavage furro

67 ested CR components, is anchored at the cell midzone, and this physical property is likely to account

68 midbodies are both absent from the elongated midzones, and actin filaments from the furrow cortex are

69 Caenorhabiditis elegans disrupt the spindle midzone (anti-parallel microtubules and associated prote

70 dle are selected and organized into a stable midzone array are poorly understood.

71 rincipal role in organizing the antiparallel midzone array, we sought to clarify their roles in monop

72 mbly as they do in spindles, whereas two key midzone assembly factors, Aurora B and Kif4, act as they

73 Therefore, in contrast to the linear midzone assembly pathway, centralspindlin and the CPC ma

74 ates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.

75 microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends.

76 D-1 may play an early role in the pathway of midzone assembly.

77 A midzone-associated Aurora B gradient was found to monito

78 or protein that translocates PRC1, a spindle midzone-associated cyclin-dependent kinase substrate pro

79 In addition, we found that the Ase1 spindle midzone-associated protein is required for bipolar spind

80 We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate

81 is required for assembly of a robust spindle midzone at anaphase and for normal timings of spindle el

82 icrotubule antiparallel bundler ase1p at the midzone at anaphase B onset.

83 tes with the spindle and concentrates in the midzone at anaphase B.

84 zed onto the spindle pole bodies and spindle midzone at anaphase onset, where it persists into midana

85 the dispersion of aurora B from the spindle midzone at late anaphase; however, centromeric associati

86 10 to the spindle during anaphase and to the midzone at telophase.

87 though intact cortices, kinetochore MTs, and midzone augmentation are dispensable, this patronin-base

88 me separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient - the 'ruler'

89 sly delaying aster separation and disrupting midzone-based signaling leads to complete failure of fur

90 anner and 2) it subsequently refines spindle midzone-based signaling to restrict furrowing to a singl

91 Disrupting midzone-based signaling, by depleting conserved midzone

92 e nonkinetochore microtubules in the spindle midzone become compacted into the central spindle, a str

93 es to promote the recruitment of Ase1 to the midzone before anaphase onset and slow spindle elongatio

94 Despite this important role, many aspects of midzone biology remain unknown, including the dynamic or

95 omputer simulations demonstrate that minimal midzone bundle formation results from promoting antipara

96 How midzone bundles assemble is poorly understood.

97 tution approach, we demonstrate that minimal midzone bundles can reliably self-organize in solution f

98 arallel microtubules associate to form tight midzone bundles, as required for functional spindle arch

99 does not localize to the cortex and spindle midzone but accumulates on spindle pole microtubules to

100 dle in the presence of Feo, including at the midzone, but the antibody-induced dissociation of Feo in

101 nsverse organization initiated at the cell's midzone by 45 min after induction and progressed bidirec

102 itates the targeting of ZEN-4 to the spindle midzone by mediating the posttranscriptional regulation

103 Midzones can be considered to be platforms that recruit

104 As a result, sliding forces within the midzone cannot buckle spindle microtubules, which allows

105 rated that WISP3 protein is expressed in the midzone chondrocytes of normal adult articular cartilage

106 when trailing chromatids are present at the midzone.Chromatid segregation must be coordinated with c

107 netochores (metaphase and anaphase), spindle midzone/cleavage furrow (anaphase and telophase), and mi

108 The distribution of pPLK4 midzone/cleavage furrow and midbody positions pPLK4 to p

109 00945, prevents translocation to the spindle midzone/cleavage furrow and prevents cellular abscission

110 tubule plus-end dynamicity, and motor-driven midzone compaction and alignment.

111 tein PRC1 plays a key role in organizing the midzone complex.

112 zone-based signaling, by depleting conserved midzone complexes, results in a converse phenotype: neit

113 We found that two other midzone components also localize to the ingressing furro

114 o, it can localize to the midzone when other midzone components are depleted, suggesting that SPD-1 m

115 e spindle midzone fails to form, even though midzone components are present.

116 SPD-1 differs from other midzone components in that it is essential for the integ

117 -1 is perturbed in embryos depleted of other midzone components, yet the cytoplasmic bundles are not

118 The spindle midzone-composed of antiparallel microtubules, microtubu

119 crotubules that self-organize at the spindle midzone contributes to positioning the cell-division pla

120 w Ect2, which is associated with the spindle midzone, controls RhoA activity at the equatorial cortex

121 naling by the centrosomal asters and spindle midzone coordinately directs formation of the cytokineti

122 pression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells.

123 e show that loss of function of spd-1 causes midzone disruptions, although cytokinesis generally comp

124 e spindle midzone, thereby promoting spindle midzone dissolution.

125 It associates with the spindle midzone during anaphase and concentrates to a midbody ma

126 anaphase and is concentrated at the spindle midzone during anaphase and telophase.

127 parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophas

128 plus-ends of interpolar microtubules at the midzone during anaphase, a process that requires survivi

129 nd then largely redistributes to the spindle midzone during anaphase, transitioning from stretches al

130 ved along interpolar microtubules and at the midzone during anaphase.

131 e mutually dependent for localization to the midzone during cytokinesis.

132 ulate along microtubules towards the spindle midzone during late anaphase.

133 localized to the chromosomes and then to the midzone during mitosis, but the mutated forms are detect

134 s, which functions in organizing the spindle midzone during mitosis.

135 amics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition.

136 In the absence of KIF4, midzones elongate abnormally, and their overlap regions

137 h regulation appears to occur by terminating midzone elongation at a specific time during cytokinesis

138 KIF4 is required to terminate midzone elongation in late anaphase.

139 ess but subsequently regress and the spindle midzone fails to form, even though midzone components ar

140 Under these conditions, the spindle midzone fails to organize and function properly.

141 indlin to function as a regulator of spindle midzone formation and as an activator of RhoA at the equ

142 anslocation of PRC1 by Kif4 is essential for midzone formation and cytokinesis.

143 logues of CHO1 were shown to function in the midzone formation and cytokinesis.

144 Kif4 knockdown results in defective midzone formation and longer spindles, revealing new ana

145 PRC1 translocation and midzone formation can be restored, and the cytokinetic d

146 Midzone formation is also inhibited in fly spermatocytes

147 bition of PRC1 translocation, a block of the midzone formation, and a failure of cytokinesis.

148 had defects in chromosome behavior, spindle midzone formation, and cytokinesis.

149 lts, in light of the crucial role of PRC1 in midzone formation, indicate that cell cycle-dependent tr

150 ralspindlin in a linear pathway that governs midzone formation.

151 hromosome-microtubule attachment and spindle midzone formation.

152 Spindle midzone fragments not connected to either of the two spi

153 The spindle midzone harbors both microtubules and proteins necessary

154 brane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), i

155 strates colocalize with Ipl1p to the spindle midzone, identifying additional proteins that may regula

156 indle translocate continuously away from the midzone in a phenomenon called poleward microtubule flux

157 Cyclin B1-Cdk1 localized at the spindle midzone in an Aurora B-dependent manner, with incomplete

158 t not condensin II, localizes to the spindle midzone in anaphase and to the midbody during normal cyt

159 s at the spindle pole bodies and the spindle midzone in anaphase cells.

160 t block Aurora B localization to the spindle midzone in anaphase or Aurora B function in cytokinesis.

161 pindle poles in metaphase and to the spindle midzone in anaphase.

162 gulating the spatiotemporal formation of the midzone in HeLa cells.

163 trolling the spatiotemporal formation of the midzone in human cells.

164 y-terminal truncation fails to form a stable midzone in late anaphase, produces higher elongation rat

165 protein, Ndc10p, is enriched at the spindle midzone in late anaphase.

166 inhibiting Plk1 caused premature assembly of midzones in cells still in metaphase, breaking the tempo

167 erial and resemble similar foci in monopolar midzones in cells.

168 orylated at centromeres and anaphase spindle midzones in vivo.

169 ink antiparallel microtubules at the spindle midzone including BIMC, MKLP, and CENP-E are closely rel

170 ring proteins on the equatorial cortex in a midzone-independent manner and 2) it subsequently refine

171 at an outward force generated by the spindle midzone, independently of centrosomes, is sufficient to

172 ued the localization of ZEN-4 at the spindle midzone, indicating that ATX-2 mediates the localization

173 localizes to the mitotic spindle and spindle midzone, indicating that SUMO paralogs regulate distinct

174 The microtubule midzone is able to stimulate the cortex of the cell to e

175 targeting of centralspindlin complex to the midzone is abrogated upon Hook2 depletion; accordingly,

176 her hand, the localization of the CPC in the midzone is independent of the borealin-HP1 interaction,

177 se1p at microtubule plus ends at the spindle midzone is more stable.

178 These studies reveal that the spindle midzone is necessary and sufficient for the stabilizatio

179 bundle of microtubules known as the spindle midzone is rapidly assembled after anaphase onset, recru

180 ted secretion in the vicinity of the spindle midzone is required for the execution of the terminal ph

181 Recent observations suggest that the spindle midzone is required for this process.

182 The midzone is the domain of the mitotic spindle that mainta

183 ure that cleavage occurs through the spindle midzone is unknown.

184 er metaphase, and accumulates at the spindle midzone late in anaphase.

185 ne plus-end dynamics whose activity controls midzone length but not stability.

186 KIF4-mediated midzone length regulation appears to occur by terminatin

187 and recruits Bim1 to the midzone to maintain midzone length.

188 spindle microtubule polymerization, limiting midzone length.

189 crotubule (MT) motor proteins at the spindle midzone, less is known about how microtubule-associated

190 the short Ase1 isoforms impairs the spindle midzone localization of full-length Ase1, it is likely t

191 The midzone localization of SPD-1 is perturbed in embryos de

192 Feo homologues in other systems enhance the midzone localization of the MT-MT cross-linking motors k

193 oss-linking motors kinesin-4, -5 and -6, the midzone localization of these motors is respectively enh

194 sequesters centralspindlin onto the spindle midzone, making it unavailable for Aurora B at the equat

195 MT-dependent networks of spindle midzone MAPs may be one molecular basis for the postulat

196 the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle

197 l metaphase but subsequently transfer to the midzone microtubule array and the equatorial cortex duri

198 In addition, midzone microtubule bundles became destabilized and auro

199 iRNA abolishes this actin-myosin-independent midzone microtubule compaction.

200 rwent chromosome poleward movement, formed a midzone microtubule complex, and completed cytokinesis.

201 es both the exaggerated anaphase and reduced midzone microtubule density observed in early neural epi

202 Investigating midzone microtubule dynamics has been difficult in part

203 addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microt

204 Midzone microtubule stabilization also depends on the ki

205 integral for coupling furrow ingression with midzone microtubule stabilization.

206 lically associates with astral, spindle, and midzone microtubules and also to actomyosin pseudocleava

207 issociates from centromeres and localizes to midzone microtubules and the equatorial cortex.

208 We suggest that cultured cells use midzone microtubules as the primary signaling pathway fo

209 crotubules at metaphase, then relocalizes to midzone microtubules at anaphase and regulates central s

210 naphase, hRif1 aligned along a subset of the midzone microtubules between the separating chromosomes.

211 ith centromeres during prometaphase and with midzone microtubules during anaphase and is required for

212 es throughout mitosis, organizes the spindle midzone microtubules during anaphase, and assembles into

213 the midbody matrix surrounding the compacted midzone microtubules during cytokinesis.

214 dback between aurora B kinase activation and midzone microtubules generates a gradient of post-transl

215 n metaphase and anaphase and associates with midzone microtubules in anaphase and telophase.

216 ssibility that microtubule polymerization of midzone microtubules is continuously required to sustain

217 mEg5 punctae on midzone microtubules moved transiently both toward and a

218 egulated by both astral microtubules and the midzone microtubules of the mitotic apparatus.

219 evealed that mEg5 punctae on both astral and midzone microtubules rapidly bind and unbind.

220 However, motion of Eg5 on midzone microtubules was not altered.

221 ividing cells, caused the disorganization of midzone microtubules, and resulted in abortive cytokines

222 segregation of chromosomes, the bundling of midzone microtubules, and the initiation of cytokinesis

223 subsequent appearance of fluorescence along midzone microtubules, but not that near the lateral equa

224 former delivered centromeric aurora B along midzone microtubules, whereas the latter delivered cytop

225 known, including the dynamic organization of midzone microtubules.

226 depend on the mitotic spindle apparatus and midzone microtubules.

227 r telophase II, becoming associated with the midzone microtubules.

228 localization of the ZEN-4 kinesin protein to midzone microtubules.

229 pindle poles and later in development on the midzone microtubules.

230 and became concentrated at the equator along midzone microtubules.

231 with centromeres or initial relocation onto midzone microtubules.

232 e equator by the centralspindlin complex and midzone microtubules.

233 pindlin complex that concentrates on spindle midzone microtubules.

234 In the spindle midzone, microtubules from opposite half-spindles form b

235 Deriving from midzones, midbodies are organized by a set of microtubul

236 ce of Arl3 in centrosomes, mitotic spindles, midzones, midbodies, and cilia are all supportive of rol

237 and Kif4 and PRC1 colocalize on the spindle midzone/midbody during anaphase and cytokinesis.

238 okinesis, but it is essential for the proper midzone/midbody formation and cytokinesis in mammalian c

239 s enriched on chromatin bridges and near the midzone/midbody in an AIR-2-dependent manner.

240 to an anti-parallel array termed the spindle midzone (midzone MTs), whereas F-actin and non-muscle my

241 on that is important for cytokinesis and for midzone MT stabilization following furrow ingression.

242 We show here that midzone MT stabilization is dependent on actomyosin cont

243 ous studies in somatic cells have shown that midzone MTs become highly stable after furrows have begu

244 ach marks showed that sliding of overlapping midzone MTs was responsible for the elongation of the sp

245 Stabilization of midzone MTs with low amounts of Taxol rescues cytokinesi

246 i-parallel array termed the spindle midzone (midzone MTs), whereas F-actin and non-muscle myosin II,

247 ads to the rapid depolymerization of spindle midzone MTs.

248 n NoCut, Aurora kinase (Ipl1) at the spindle midzone negatively regulates cytokinesis through two pro

249 in TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells.

250 hibited greatly enhanced localization in the midzone of developing phragmoplast.

251 pression of the 3 proteins extended into the midzone of OA cartilage.

252 s in mitotic cells where it localizes to the midzone of the spindle during anaphase, and to the cleav

253 t form in the absence of associated spindle, midzone, or chromosomes.

254 Disruption of AIR-2, the spindle midzone, or condensin leads to cytokinesis failure in a

255 ule-associated proteins (MAPs) contribute to midzone organization and function.

256 ion, it is unknown whether interference with midzone organization by EWSR1/FLI1 induces aneuploidy.

257 ation pattern, demonstrating dynamic spindle midzone organization in oocytes.

258 chromosomal passenger complex (CPC) promotes midzone organization, specifies the cleavage plane, and

259 that affect chromosome behavior and spindle midzone organization.

260 mployed monopolar cytokinesis to reveal that midzone plus ends appear to be nondynamic.

261 hromokinesin KIF4 as a negative regulator of midzone plus-end dynamics whose activity controls midzon

262 report in Science a mechanism of asymmetric midzone positioning driven by a polarized cortical distr

263 s critical to ensure that furrow and spindle midzone positions coincide throughout cytokinesis.

264 ess, we found that Ase1, a conserved spindle midzone protein [5], appeared as two short protein isofo

265 We show that the spindle midzone proteins kinesin-5 cut7p and MT bundler ase1p co

266 them, focusing on the conserved cytokinesis midzone proteins Prc1 and Kif4A.

267 neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger co

268 f cytokinesis and speculate that the spindle midzone region of animal cells is functionally equivalen

269 anaphase, binding to RacGAP1 at the spindle midzone repositions Ect2 to induce local actomyosin ring

270 cumulation of centralspindlin at the spindle midzone requires its multimerization into clusters and A

271 ock-down of PRC1, which disrupts the spindle midzone, revealed the existence of two pathways that can

272 t Ase1p is a member of a conserved family of midzone-specific MAPs.

273 ochores in early mitosis and shuttles to the midzone spindle at mitotic exit.

274 nd separation, as well as recruitment of key midzone-stabilizing proteins all appeared normal, but mi

275 The spindle midzone stimulates the actomyosin-driven contraction of

276 that both actin binding and MKlp2-dependent midzone targeting cooperate to precisely position the CP

277 nterdigitating microtubules to establish the midzone that is necessary for cytokinesis.

278 s the localization of Aurora B kinase to the midzone (the midline structure located between segregati

279 Once at the midzone, the CYK-4 subunit functions to recruit actin re

280 It directs vesicles to the midzone, the site where they coalesce to form the new ce

281 f extra-long chromatids and clearance of the midzone, thereby avoiding cytokinesis failure and aneupl

282 tor composition of antiparallel ipMTs at the midzone, thereby facilitating the kinesin-5-driven slidi

283 isassembly factor to localize at the spindle midzone, thereby promoting spindle midzone dissolution.

284 release of centralspindlin from the spindle midzone through inhibition of PRC1, allowing centralspin

285 yos, showing that Ect2 migrates from spindle midzone to astral microtubules in anaphase and that Ect2

286 ng protein EB1/Bim1 and recruits Bim1 to the midzone to maintain midzone length.

287 antiparallel microtubules within the spindle midzone to play roles in bipolar spindle assembly and pr

288 cal nuclear envelope breakdown to the bridge midzone to prevent the leakage of material from daughter

289 over length scales of micrometres, from the midzone to the cell cortex, is not known.

290 egregate the chromosomes before anaphase and midzones to keep sister genomes apart and guide the clea

291 Also, it can localize to the midzone when other midzone components are depleted, sugg

292 -2 to chromosomes and perhaps to the spindle midzone, where AIR-2 phosphorylates proteins that affect

293 h it congresses inward from the poles to the midzone, where it becomes progressively enriched at regi

294 se, the kinase Plk1 localizes to the spindle midzone, where it orchestrates cytokinesis.

295 ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus

296 Feo increases kinesin-5 association with the midzone, which becomes abnormally narrow, leading to imp

297 esis regulators that localize to the spindle midzone, which forms between the separating chromosomes.

298 ons activated Aurora B(AIR-2) at the spindle midzone, which is needed for the abscission checkpoint i

299 mmetric positioning of the telophase spindle midzone, which specifies the cleavage furrow.

300 hat it is essential for the integrity of the midzone, yet not for cytokinesis.