ライフサイエンスコーパス: minigene

1 APII, and the splicing factor SF2/ASF at the minigene.

2 TTC repeats into an intron of a GFP reporter minigene.

3 ing and translational inhibition from a DICE-minigene.

4 tive splicing and exon inclusion from a CD44 minigene.

5 n 7 inclusion in mRNAs derived from the SMN2 minigene.

6 der the control of a human Cathepsin G (hCG) minigene.

7 ative poly(A) site selection on the reporter minigene.

8 atory elements in the alpha-tropomyosin test minigene.

9 e endogenous gene, as well as from an IKBKAP minigene.

10 elta147) when co-expressed as an ER-targeted minigene.

11 sis of a 51-nt internal exon in a three-exon minigene.

12 ess-induced alternative splicing of the MDM2 minigene.

13 to be an independent event localized to the minigene.

14 licing efficiency of the introns of the MDM2 minigene.

15 cts the splicing of a damage-responsive MDM2 minigene.

16 protein 1 and regulates splicing of reporter minigenes.

17 nsfection of G(o1) minigenes but not G(i1/2) minigenes.

18 er copy-number dependence than observed with minigenes.

19 g RNA analysis of human samples and splicing minigenes.

20 ion of the SC35 binding site in the PKCdelta minigene abolished RA-mediated utilization of 5' splice

21 , which, when introduced into the human CFTR minigene, abolished exon 9 skipping.

22 ion of a 395 bp upstream region in the MOR23 minigene abolishes expression.

23 DC pulsed with TAT-minigene also efficiently induced Flu-M1-specific T cell

24 Induction of the AUA minigene also stimulates both leftward and rightward fra

25 iates with cyclin D1b mRNA (transcript-b) in minigene analyses and with endogenous transcript in pros

26 Minigene analyses demonstrated that this alteration prec

27 Bioinformatic and minigene analyses identified signals that could modify t

28 Minigene analysis showed that the resultant net loss of

29 To test this idea, we constructed a CFTR minigene and replicated exon 9 skipping associated with

30 ecrease exon 10 inclusion in a wild type tau minigene and rescue the increase in exon 10 splicing cau

31 , we cloned a heterologous splicing PKCdelta minigene and showed that inclusion of PKCdelta exon 9 is

32 ge-specific alternative splicing of the MDM2 minigene and that the splicing factor SRSF1 binds exon 1

33 n IIIb inclusion in transcripts derived from minigenes and from the endogenous FGF-R2 gene.

34 eceptors was further combined with G protein minigenes and pharmacological intervention, along with c

35 Transfection of cells with single-exon IIIb minigenes and single-exon IIIc minigenes revealed that m

36 of COL2A1; construction of splicing reporter minigenes and transfection into cultured cells; and RT-P

37 hin two different internal exons in a 3-exon minigene, and millions of successfully spliced transcrip

38 n, the dominant negative Galpha(q)-(305-359) minigene, and pretreatment with pertussis toxin inhibite

39 ion on pre-mRNA splicing was studied using a minigene approach.

40 Here we show that the mouse and human minigenes are regulated similarly by conserved elements

41 cient to induce exon 7 skipping in the mouse minigene as in the human SMN2.

42 ependent exon 6 exclusion from fas mRNA in a minigene assay in cells.

43 Genetic advances include the use of the minigene assay to confirm pathogenicity of splice site m

44 Of 100 BRCA2e3 variants tested in the minigene assay, 64 were found to be spliceogenic, causin

45 itative real-time polymerase chain reaction, minigene assay, and flow cytometry.

46 Using a splicing reporter minigene assay, we demonstrated that RBM4 promoted exon

47 Using a minigene assay, we showed that c.790A>G altered CIZ1 spl

48 335A increases TDP-43 splicing function in a minigene assay.

49 haracterized for their impact on splicing in minigene assays and, when available, in patient lymphobl

50 Minigene assays in NIH3T3 cells further confirmed that a

51 Minigene assays were performed for two non-canonical spl

52 his variant completely prevented splicing in minigene assays, and resulted in exon skipping and an in

53 CLRN1 minigene-based analysis confirmed the splicing of an abe

54 t Wt1 promotes exon inclusion using a Vegf-a minigene-based splicing assay.

55 Minigene-based splicing assays upon MALAT1 modulation re

56 We show that a DUX4 minigene, bearing only the homeodomains and C-terminus,

57 We also identified two dipeptide coding minigenes between the Shine-Dalgarno sequence and start

58 nd could be blocked by transfection of G(o1) minigenes but not G(i1/2) minigenes.

59 When introduced as a minigene by retroviral transduction into human breast ca

60 pattern of LH2, both endogenously and in the minigene, by the RNA-binding splicing proteins TIA-1 and

61 ly, we have shown that use of the C-terminal minigenes can specifically block receptor activation of

62 Using minigenes carrying deletion mutations within intron 11,

63 ct of high-level expression of two different minigenes carrying either the rpoB-2 or the ndhF-2 editi

64 HEK293T cell transduction with minigenes carrying exon 39 showed that the splice defect

65 Using minigenes carrying linker-scanning mutations within exon

66 Transfection of minigenes carrying mutations associated with DRRD (G118R

67 Using a CoAA minigene cassette, we find that the switched alternative

68 in D3-induced expression of a growth hormone minigene cassette.

69 and rapid approach based on a modular hybrid minigene combined with antisense oligonucleotides to ena

70 The shark kappa clusters are minigenes consisting of a simple VL-JL-CL array, where V

71 In transgenic mice, a minigene construct containing 10 kb of upstream and 1.5

72 An FLT1 minigene construct containing exon 13, 14 and the interv

73 COS-1 and HepG2 cells transfected with a F5 minigene construct containing the patient's mutation exp

74 ption of dimerization using a TM4 peptide, a minigene construct encoding TM4, or by mutation of TM4,

75 Mice immunized with a minigene construct of hgp100(25-33) rejected B16 melanom

76 A minigene construct showed that this mutation alters spli

77 s faster and less sensitive to a PLCbeta1-ct minigene construct than inhibition of PIP(2)-sensitive K

78 We have engineered a human tau minigene construct that was designed to allow alternativ

79 gulate alternative splicing using a splicing minigene construct transfected into the oligodendrocyte

80 tion of Bcl-x pre-mRNA, we developed a BCL-x minigene construct which conferred the same ratio of Bcl

81 moter upstream of a human lipoprotein lipase minigene construct with a glycosylphosphatidylinositol a

82 e development using a recombinant protein or minigene construct, a series of truncated recombinant RA

83 equences, when transferred to a heterologous minigene construct, inhibited splicing but only when clo

84 rted at different positions within the FGFR2 minigene construct.

85 Human FIX minigene constructs containing a simian virus 40 (SV40)

86 In vitro assays were performed with minigene constructs containing ABCA4 exon 39.

87 Minigene constructs containing the entire intron 4 seque

88 In Drosophila melanogaster S2 cells, using minigene constructs designed to produce nearly identical

89 ssays between Xenopus laevis and X. borealis minigene constructs injected into oocytes.

90 Minigene constructs of the HD locus confirm the regulato

91 AT3 tumors in syngeneic rats, we constructed minigene constructs that report on alternative splicing.

92 re assessed by transfecting COS-7 cells with minigene constructs.

93 n of an alternative exon is through reporter minigene constructs.

94 tic), a finding reproduced in vitro by using minigene constructs.

95 and a collection of experimentally clarified minigene constructs.

96 us MYPT1 as well as stably transfected MYPT1 minigene constructs.

97 DNA ends (RACE), and transient expression of minigene constructs.

98 is of potential SRSF10 binding motifs in two minigene constructs.

99 d accumulation of both mRNA expressed from a minigene containing exon 12 and the endogenous G6PD mRNA

100 nusoidal endothelial cells by transfecting a minigene containing the EIIIA exon and its flanking intr

101 COS cells transfected with a minigene containing the intronic +14 mutation produce ex

102 pha-spectrin gene transcripts derived from a minigene containing the IVS 22 +5 mutation.

103 sgenic mouse model using a 2.9-kilobase HD-5 minigene containing two HD-5 exons and 1.4 kilobases of

104 this effect was not specific to ETR-3 since minigenes containing either of the two motifs were respo

105 Minigenes containing X. laevis spacer sequences are only

106 Mutational analysis of an LH2 minigene demonstrated that 2 of the 4 Fox binding motifs

107 ation with a VV-e7r-expressing ubiquitinated minigene, demonstrated that memory CD8 T cells alone cou

108 rnative splicing, we systematically screened minigenes derived from the GABA(A)Rgamma2 and human beta

109 demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhance

110 hereas mice immunized with the mgp100(25-33) minigene did not develop protective tumor immunity.

111 competing 5' splice sites in an alpha-globin minigene, direct hnRNPH/F-regulated alternative splicing

112 The expression of a FVII minigene directed by a segment of the native FVII promot

113 A hybrid minigene driven by cytomegalovirus promoter mimicked 1B-

114 ransgenic line of mice carrying a tyrosinase minigene driven by the dopachrome tautomerase (Dct) prom

115 cinar cells in transgenic mice by creating a minigene driven by the rat elastase I enhancer/promoter.

116 CD8(+) T cell lines generated to minigene-encoded CTL epitopes secreted IFN-gamma and TNF

117 5-HT was antagonized by the expression of a minigene encoding a peptide scavenger of Gbetagamma subu

118 Here, we engineered a DNA minigene encoding seven H-2(b)-restricted Cpn CTL epitop

119 n PTLDKVLEV was pulsed onto cells, or when a minigene encoding this sequence was used to artificially

120 esponses by vaccinating rhesus macaques with minigenes encoding fragments of Gag, Vif, and Nef.

121 ma and beta-arrestin proteins; expression of minigenes encoding the carboxyl terminii of the G protei

122 ed whether vaccinating rhesus macaques with "minigenes" encoding fragments of Gag, Vif, and Nef resul

123 ith AGA at the test position; induction of a minigene ending in AGA stimulates bypassing at the latte

124 We find that induction of a minigene ending in AUA stimulates bypassing at an AUA co

125 Minigene experiments demonstrated an important role for

126 Minigene experiments explain disease-associated mutation

127 Reporter minigene experiments investigating representative exons

128 ional Ub from a poly hemagglutinin-tagged Ub minigene expressed from the Hprt locus.

129 sense mutations W1001X and R1014X using hERG minigenes expressed in HEK293 cells or neonatal rat vent

130 e containing ECR2 reproducibly directed lacZ minigene expression in mesoderm.

131 A minigene expression system confirms both the effect of t

132 temporal neocortex tissue and in a cellular minigene expression system.

133 h delivered one exon of a beta-galactosidase minigene flanked by donor or acceptor splice sequences.

134 We created minigenes for two of these genes, the CFTR and MTMR1 gen

135 Expression of minigene fragments from beta-arrestin1 or STAM1, known t

136 the IghPax5/+ mouse, which expressed a Pax5 minigene from the immunoglobulin heavy-chain locus.

137 e constructed various tissue-specific unc-52 minigenes fused to a gene for green fluorescent protein

138 In contrast, DC pulsed with minigene fusion protein lacking TAT were either poorly r

139 A TAT-minigene fusion protein was generated, encoding both the

140 ally, the haplotype- and allele-specific ACE minigenes generated similar splicing patterns in both AC

141 Minigenes generated with pSpliceExpress show the same re

142 Transfection of a G(i1/2) minigene had no effect on thrombin-stimulated [Ca(2+)](i

143 We expressed minigenes harboring the mutations in cell lines to demon

144 ertion of another RNAPII pause site into the minigene has a similar effect on exon IIIb silencing.

145 evis spacer sequences are only dominant over minigenes having complete X. borealis spacers if a space

146 In addition, a Gbetagamma minigene, hbetaARK1(495), inhibits VPF/VEGF-stimulated H

147 Functional assays using splicing reporter minigenes identified the proteins hrp36 and hrp38 and th

148 in an immunogenic context; that is, as a DNA minigene in a bacterial carrier system.

149 a calcitonin/calcitonin gene-related peptide minigene in a hormone-dependent manner.

150 urvival, whereas re-introduction of an LRP-1 minigene in a mouse LRP-1-deficient fibroblast cell line

151 vel of mutant mRNA compared to the wild-type minigene in an NMD-dependent manner.

152 ate that the unplanned expression of the hGH minigene in CollagenVI expressing mesenchymal cells can

153 Splicing assays using a transfected TP53 minigene in combination with siRNA knockdown of SRSF3 sh

154 Expression of a minigene in LX2 containing the AZIN1 slow-fibrosis SNP y

155 xpressed in the same cell type as the unc-52 minigene in order to regulate its expression, supporting

156 findings by analyzing the effect of the hGH minigene in TgC6hp55 transgenic mice which express the h

157 vates exon inclusion of a cardiac troponin T minigene in transient transfection assays in an MSE-depe

158 short:long isoform-expression ratios of DRD2 minigenes in cell culture.

159 nisms are typically studied using artificial minigenes in cultured cells, conditions that may not acc

160 ctivate exon inclusion of cardiac troponin T minigenes in vivo via muscle-specific splicing enhancer

161 the MHC class I-dependent CD8(+) T cells to minigene-included Cpn CTL epitopes, rather than by pan-D

162 The minigene integrated 0.98 Mb upstream of Sox9 and was acc

163 s substantiated by transfection of the G(o1) minigene into HMECs, which led to a blockade of thrombin

164 ting of a single-copy gp91(phox) therapeutic minigene into one allele of the "safe harbor" AAVS1 locu

165 trastromal injection of a lumican expression minigene into the corneal stroma of Lum-/- mice.

166 e this hypothesis, we first transfected LDLR minigenes into SH-SY5Y neuroblastoma cells and found tha

167 s are here recapitulated by MOR23 and M71 OR minigenes, introduced into mice.

168 Therefore, introduction of a functional CD45 minigene is sufficient to overcome the principal severe

169 ability to direct this process by the use of minigenes is relevant to the design of vaccines and unde

170 A synthetic minigene library with degenerate regions in 3' intronic

171 into an intron in the APRT gene or the HPRT minigene, long tracts of CTG/CAG repeats (more than abou

172 To this end, we designed a minigene (M), and in combination with a series of deleti

173 e approaches, termed the "heterozygous" and "minigene" methods, we generated mice in which Cre-expres

174 exon 10-skipped CYP24A1 splice variant; in a minigene model the latter was attenuated by a functional

175 Expression of one of the minigenes modestly interfered with translation of ycbK.

176 Using high-throughput genetics, 5560 minigene molecules were assayed for splicing in human HE

177 Minigenes of 9 kb and as short as 2.2 kb are selectively

178 A panel of minigenes of varying splicing potential were integrated

179 idenced by investigating the effect of G(o1) minigenes on thrombin-stimulated stress fiber formation

180 n 7 inclusion in mRNAs derived from the SMN2 minigene or from endogenous SMN2.

181 icing errors of either the mutated human DDC minigene or the mouse artificial splicing construct in v

182 Manipulation of the UCE, in a reporter minigene or via random mutations in the genomic context

183 sigma, and sigma-2, encoded by 35 functional minigenes or clusters.

184 nd LFA-3, together with either p540 and p865 minigenes or the full-length hTERT, effectively stimulat

185 6, by expression of a dominant negative TP53 minigene, or by TP53 depletion.

186 s were found when autologous hFIX cDNA, hFIX minigenes, or hPC minigenes were used as reporters in th

187 tion, these findings highlight the generated minigene panel as a flexible platform for the query of s

188 ylation was equivalently detected across the minigene panel, irrespective of splicing and H3K36me3 st

189 patS minigenes patS4 to patS8 encode PatS C-terminal 4 (GSGR)

190 and an engineered alpha-SNAP binding-domain minigene peptide blocked basal and evoked vWF secretion.

191 i) response to the same extent as with G(o1) minigene peptide, suggesting that this G(o)-mediated [Ca

192 We have now used the 'minigene' phenomenon to ascertain whether depletion of t

193 transcripts, the transfection of the A or C minigenes produced a large amount of the alternatively s

194 l peptide (PA(224-233)) expressed as a viral minigene product formed a sizeable cytosolic pool contin

195 rther, co-transfection of SC35 with PKCdelta minigene promoted selection of 5' splice site II.

196 The GFP_(GAA*TTC)(560) minigene recapitulates the molecular hallmarks of the mu

197 HITS-CLIP and minigene reporter analyses indicate that these polyadeny

198 r adjacent to 2,198 human exons in the MFASS minigene reporter and found that 3.8% (1,050) of variant

199 of that SNP on alternative splicing using a minigene reporter assay.

200 In one gene (MAGOH), using minigene reporter assays, we demonstrated that the combi

201 Using a minigene reporter, we show that Sam68 controls expressio

202 dulate alternative exon inclusion from a MAG minigene reporter.

203 Two minigene reporters were constructed: One produces green

204 * and 33 can be recapitulated in transfected minigene reporters.

205 ant vaccinia virus expressing epitope V as a minigene, resulted in the induction of weak, but reprodu

206 ection of this cell line with a murine Xylt2 minigene results in the production of recombinant chondr

207 High level expression of plasmid-borne minigenes results in the sequestration as peptidyl-tRNA

208 of Akt2(-/-) cells with a PKCbetaII splicing minigene revealed defective betaII exon inclusion.

209 gle-exon IIIb minigenes and single-exon IIIc minigenes revealed that mutation of terminal sequences o

210 Minigene RNA splicing studies in BV2 microglial cells es

211 man fibroblasts (HFFs) expressing a Target 1 minigene RNA, which contains the required splicing and P

212 Analyzed with minigenes, SMN1C6T displayed a approximately 20% increas

213 Minigene splicing analyses revealed that the AMELX misse

214 lly, changing the promoter alters both FGFR2 minigene splicing and the MAZ4 effect.

215 to prioritize VUS and developed a cell-based minigene splicing assay to confirm aberrant splicing.

216 ns and demonstrates functional activity in a minigene splicing assay.

217 LW/MW exon 3 haplotypes by semi-quantitative minigene splicing assay.

218 und to have an impact on splicing by using a minigene splicing assay.

219 Reticulocyte RNA and functional minigene splicing assays in heterologous cells revealed

220 Minigene splicing assays revealed a novel splicing event

221 In silico predictions, minigene splicing assays, patients' RNA analyses, a mous

222 These predictions were confirmed using minigene splicing assays.

223 Using a minigene splicing construct containing an SVA, we observ

224 Mutating iE(B) in minigene splicing reporters abrogated intrasplicing, as

225 In vitro minigene splicing studies qualitatively replicated these

226 Through chimeric minigenes splicing assay we investigated the unique elem

227 Using bacterial artificial chromosome-minigene stable cell lines, CRISPR/Cas9 enhancer-deleted

228 In vitro minigene studies and in vivo splicing analyses revealed

229 Finally, data that we obtained using minigenes suggest that tim alternative splicing might ac

230 o levels comparable to that of the wild-type minigene, suggesting that hERG nonsense mutations are su

231 of T1945+6C on splicing was studied using a minigene system and on function by heterologous expressi

232 Using an in vitro minigene system to analyze splicing events, we found red

233 In this manuscript we use an inducible minigene system to determine the time course of ASO acti

234 We used a minigene system to study two frameshift mutations, R1032

235 Using a CFTR minigene system, we studied TG tract variation and obser

236 at exon-intron boundaries using an in vitro minigene system.

237 Using a novel tyrosinase minigene-tagged Sleeping Beauty transposon-mediated muta

238 A dominantly acting K14-agouti minigene tags both rearrangements, which enables these b

239 Using a minigene that includes exon 4, intron 4, exon 5, intron

240 region, we replaced the region with a 659-bp minigene that linked the three cis-elements (MG-GFP).

241 DM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2 splicing in respons

242 We have designed a G alpha(s) minigene that retains the signals required for G alpha(s

243 Using an intron 8-inclusion minigene that supports NMD, we demonstrated that mutant

244 this issue of Neuron, Vassalli et al. use OR minigenes that coexpress histochemical markers and show

245 ling in endothelial cells, we have developed minigenes that encode an 11-amino acid C-terminal peptid

246 NMD was studied using minigenes that support NMD.

247 A of tRNA cognate to the last triplet of the minigene, thereby limiting protein synthesis for lack of

248 classical memory responses were presented as minigenes, they induced clear memory inflation.

249 For the CFTR minigene this regulation was demonstrated to be dependen

250 We delivered these minigenes through combinations of recombinant Mycobacter

251 ls (LS8) were transfected with an amelogenin minigene to increase amelogenin synthesis, the transfect

252 We used a series of HIV-1 reporter minigenes to demonstrate that Tat's role in splicing is

253 In this study, we screened 366 human miRNA minigenes to determine their effects on the major signal

254 Several recent reports use fluorescence minigenes to image alternative splicing events in living

255 h the aid of p115RhoGEF-RGS, G(12) and G(13) minigenes to inhibit G(12/13), we found that the G(12/13

256 Whereas transfection of the G or T minigenes transcribed predominantly normal-sized transcr

257 stituted U2AF2 protein reduces splicing of a minigene transcript carrying prototypical splice sites.

258 cells resulted in an increase in LH2 (long) minigene transcripts, accompanied by a decrease in LH2 (

259 (LDLR) exon 12 in female human liver and in minigene-transfected HepG2 cells.

260 in both in vitro complementation assays and minigene-transfected mouse erythroleukemia cells (MELCs)

261 Using a minigene transfection assay for alternative splicing act

262 Plasmid DNA encoding a FN-C/H-II minigene under control of the cytomegalovirus promoter w

263 c mice have been generated that carry a CD45 minigene under control of the human leukocyte function-a

264 Transfection splicing assays of SMN minigenes under hypoxia revealed that hypoxia-induced sk

265 The human growth hormone (hGH) minigene used for transgene stabilization in mice has be

266 CD45 minigenes using the CD45 5' sequences up to 19 kilobases

267 0-fold increase in the Cpn challenging dose, minigene-vaccinated mice had a 60-fold reduction in lung

268 GD2 cross-reactive IgG antibody responses by minigene vaccination with a protective epitope of GD2 ga

269 chieved for the first time using an oral DNA minigene vaccine against murine vascular endothelial gro

270 s for inclusion in a multiepitope peptide or minigene vaccine construct, truncated recombinant Hsp20

271 this model by demonstrating that an oral DNA minigene vaccine induces effective HLA-A2-restricted, CE

272 This minigene vaccine strategy provides a more flexible alter

273 mice was broken by the CEA(691) (IMIGVLVGV) minigene vaccine.

274 pertussis toxin or cells transfected with a minigene vector for Gi inhibited the ISO-mediated RhoA a

275 ARMS2 transcription from minigene vectors carrying different alleles at variants

276 However, cells transfected with minigene vectors for G12 and G13 did not prevent RhoA ac

277 simple presentation of the Ag in the form of minigene vectors.

278 binant vaccinia virus encoding SSIEFARL as a minigene (VvgB(498-505)).

279 ents that influenced Bcl-x splicing, a Bcl-x minigene was constructed.

280 An LH2 minigene was designed, validated, and used in Fox-2 over

281 Further analyses showed that the hGH minigene was expressed in several tissues, also leading

282 nd a keratin-14 (K14) promoter-driven agouti minigene was introduced onto the inverted chromosome 15

283 lternative 5' splice site utilization in the minigene was promoted by RA.

284 3' intronic regions (3.4 million individual minigenes) was used to compare BP usage of SF3B1K700E an

285 Using an LH2 minigene, we have compared the regulation of the alterna

286 Also, using an I-SceI-sensitive HPRT minigene, we show that gene correction is more efficient

287 Using Galpha13 or Galpha12 minigenes, we demonstrated that Galpha13, but not Galpha

288 Using splicing minigenes, we identified multiple exonic and intronic sp

289 Using transfected minigenes, we performed a systematic analysis of the seq

290 DC treated with TAT-minigene were efficiently recognized by both Flu-M1 and

291 patS minigenes were constructed and expressed by different de

292 A variety of P element minigenes were examined in transgenic embryos to determi

293 autologous hFIX cDNA, hFIX minigenes, or hPC minigenes were used as reporters in the expression vecto

294 Minigenes were used to validate the functional effect of

295 lved in 5' splice site selection we cloned a minigene, which included PKCdelta exon 10 and its flanki

296 Using a TP53 minigene, which mimics intron 9 alternative splicing, we

297 whereas co-injection of a lumican-expressing minigene with a beta-galactosidase reporter driven by th

298 lacement of the TG dinucleotide tract in the minigene with random sequence abolished splicing of exon

299 the deltaenalphaA promoter was maintained in minigenes with different types of introns and polyadenyl

300 The system allows generation of minigenes within one week.