ライフサイエンスコーパス: mitochondrial RNA

1 9, a factor that is known to stabilize yeast mitochondrial RNA.

2 ormed foci coincident with newly synthesized mitochondrial RNA.

3 m using Illumina deep sequencing of purified mitochondrial RNA.

4 e and dinucleotide addition in characterized mitochondrial RNAs.

5 on of non-encoded nucleotides into PHYSARUM: mitochondrial RNAs.

6 ntify, quantitate, and characterize chimeric mitochondrial RNAs.

7 ing and structural dynamics of polycistronic mitochondrial RNAs.

8 ntaining deletion mutations express chimeric mitochondrial RNAs.

9 ivity in the organelle is reconstituted with mitochondrial RNAs.

10 ions, respectively, in nascent polycistronic mitochondrial RNA(4-6).

11 ng the Pet 127 protein, which is involved in mitochondrial RNA 5' processing and degradation, also pa

12 pyrimidine triphosphate levels essential for mitochondrial RNA abundance.

13 ading to enhanced host death associated with mitochondrial RNA and DNA depletion, and lethal activati

14 t in the expression of factors implicated in mitochondrial RNA and DNA metabolism was accompanied by

15 phosphate of 2'-F-NMC is not a substrate for mitochondrial RNA and DNA polymerases, indicating that m

16 Both mitochondrial RNA and DNA syntheses were affected by rnh

17 nuclease that is involved in the turnover of mitochondrial RNA, and is essential for mitochondrial fu

18 y than the wild-type protein with endogenous mitochondrial RNAs, and that phenotype probably explains

19 that gRNAs represent only a subset of small mitochondrial RNAs, and yet an inexplicably high fractio

20 types in PS-modeled LamC mutations, as other mitochondrial RNAs are affected by inhibition of NE budd

21 Here, we report that processed mitochondrial RNAs are consolidated into micrometer-scal

22 f partial and extragenic editing in Physarum mitochondrial RNAs, as well as an additional 772 C, U an

23 Analysis of mitochondrial RNAs at single nucleotide resolution revea

24 RNA, after the physical removal of rRNA (and mitochondrial RNA), because quantitative information on

25 Recently, the multiprotein mitochondrial RNA binding complex 1 (MRB1) has emerged a

26 sequence factor 1 (GRSF1) promoter region, a mitochondrial RNA binding protein, in replication- and d

27 The mitochondrial RNA binding proteins MRP1 and MRP2 form a

28 The multiprotein mitochondrial RNA-binding complex 1 (MRB1) is emerging a

29 RECC is largely RNA-free, and accessory mitochondrial RNA-binding complex 1 (MRB1) variants serv

30 The addition of LRPPRC/SLIRP, a mitochondrial RNA-binding complex, enhanced activity of

31 Here, we examined the role of the mitochondrial RNA-binding protein GRSF1 in regulating ma

32 ng a different function in the regulation of mitochondrial RNA biology, from mRNA processing and matu

33 discuss the individual role that each has in mitochondrial RNA biology.

34 been identified in nuclear genes involved in mitochondrial RNA biology.

35 ells are potently activated by bacterial and mitochondrial RNA, but not by mammalian total RNA, which

36 f LRPPRC and PNPT1, two proteins involved in mitochondrial RNA catabolic processes and both negativel

37 urospora crassa DEAD-box protein CYT-19 is a mitochondrial RNA chaperone that promotes group I intron

38 n demonstrated for the archael, eucaryal, or mitochondrial RNAs, comparative sequence analysis has es

39 experiments have demonstrated that T.brucei mitochondrial RNAs contain both short (approximately 20

40 The mitochondrial RNA degradosome of budding yeast (mtEXO) h

41 apy synergism and experimentally validated a mitochondrial RNA-dependent mechanism for drug-induced i

42 ow that the oligoribonuclease REXO2 degrades mitochondrial RNA dinucleotides to prevent RNA-primed tr

43 n regulating the formation and resolution of mitochondrial RNA:DNA hybrids.

44 The results suggest that mitochondrial RNA editing at the cob-908 site is necessa

45 emically mutated plant population identified mitochondrial RNA editing factor 10 (MEF10) in Arabidops

46 described role of RRM-containing proteins as mitochondrial RNA editing factors.

47 related to trans-splicing, RNA granules and mitochondrial RNA editing in single-cellular trypanosome

48 2, or KREN3 endonucleases, are essential for mitochondrial RNA editing in Trypanosoma brucei.

49 ting by down-regulation of expression of the mitochondrial RNA editing TUTase 1 by RNA interference h

50 Two mitochondrial RNA editing TUTases have been described: R

51 Kinetoplastid mitochondrial RNA editing, the insertion and deletion of

52 urrently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insertion and U-deletion cy

53 as physically been depleted of ribosomal and mitochondrial RNA followed by bioinformatic steps to dif

54 Because tRNA(Gln)(UUG) is a constituent of mitochondrial RNA fractions and is encoded only by nucle

55 r complement of tRNA(Gln)(UUG) is present in mitochondrial RNA fractions, compared with 1% or less fo

56 We observe increased chimeric mitochondrial RNA frequency in samples from patients wit

57 fraction (VAF) of heteroplasmic variants in mitochondrial RNA from 257 CHD cardiovascular tissue sam

58 Mitochondrial RNA gel blot analyses indicated that all f

59 lasm maize lines, inbreds, and F1 hybrids by mitochondrial RNA gel blot analyses revealed that Rf8 is

60 stinct foci, which we have previously termed mitochondrial RNA granules (MRGs).

61 at the nucleoid and the spatially juxtaposed mitochondrial RNA granules, is protein synthesis also pe

62 2, and FASTKD5, FASTKD3 does not localize in mitochondrial RNA granules, which are sites of processin

63 Here, we discuss the recently characterized "mitochondrial RNA granules," mitochondrial subdomains wi

64 torted cristae and enlarged granules, likely mitochondrial RNA granules.

65 with the observation that h-mtTFB1 and human mitochondrial RNA (h-mtRNA) polymerase can also be coimm

66 Yeast SUV3 is a nuclear encoded mitochondrial RNA helicase that complexes with an exorib

67 aromyces cerevisiae has been classified as a mitochondrial RNA helicase.

68 FT1 insertion in supv3l1, a gene encoding a mitochondrial RNA helicase.

69 on of the import requirements indicates that mitochondrial RNA import proceeds by a pathway including

70 ion of a mammalian protein directly bound to mitochondrial RNA in vivo and provide a possible molecul

71 Northern analysis of mitochondrial RNAs in an atp25 temperature-sensitive mut

72 Mitochondrial RNAs in Trypanosoma brucei are post-transc

73 Mitochondrial RNAs in Trypanosoma brucei undergo posttra

74 d TFB2M correlated with the level of nascent mitochondrial RNA intensity (r = 0.896; P = 0.0156).

75 rniation(5) mediated by BAX and BAK releases mitochondrial RNA into the cytoplasm and triggers a RIG-

76 Maturation of Physarum mitochondrial RNA involves the highly specific insertion

77 we conclude that cytoplasmic accumulation of mitochondrial RNA is an intrinsic immune surveillance me

78 l translation and reveal that f(5)C in human mitochondrial RNA is generated by oxidative processing o

79 on of non-encoded nucleotides into PHYSARUM: mitochondrial RNAs is closely linked to transcription.

80 Mitochondrial RNA levels in general were found to be low

81 ockdown with RNA interference did not affect mitochondrial RNA levels.

82 sequent OXPHOS deficiency, without affecting mitochondrial RNA levels.

83 These properties of the mitochondrial RNA ligase are consistent with an expected

84 It was shown previously that the REL1 mitochondrial RNA ligase in Trypanosoma brucei was a vit

85 Mitochondrial RNA ligase subsequently rejoins the mRNA.

86 tochondrial precursor tRNAs, a vital step in mitochondrial RNA maturation, and is comprised of three

87 Defects in mitochondrial RNA metabolism have been linked to sensori

88 The function of MAR1 in mitochondrial RNA metabolism in L. tarentolae remains to

89 Further analysis of mitochondrial RNA metabolism revealed that the slo3 muta

90 , has been implicated in multiple aspects of mitochondrial RNA metabolism.

91 rate that these RAP proteins are involved in mitochondrial RNA metabolism.

92 the less characterized members, FASTKD3, in mitochondrial RNA metabolism.

93 As a result, failures in mitochondrial RNA metabolisms were responsible for the c

94 s of modifications at U(34) of tRNAs altered mitochondrial RNA metabolisms, causing a degradation of

95 ether, our results reveal that site-specific mitochondrial RNA modifications could be therapeutic tar

96 ble cell type in HD, we observe a release of mitochondrial RNA (mtRNA) (a potent mitochondrial-derive

97 Here, we demonstrate that mitochondrial RNA (mtRNA) also accumulates in the cytoso

98 However, the fate of mitochondrial RNA (mtRNA) during apoptosis is unknown.

99 itochondrial membrane and directly regulates mitochondrial RNA (mtRNA) homeostasis and bioenergetics.

100 Mitochondrial RNA (mtRNA) in the cytosol can trigger the

101 Mitochondrial RNA (mtRNA) polymerases are related to bac

102 uction through mechanisms that are driven by mitochondrial RNA (mtRNA) release and activation of the

103 e transmission of heteroplasmy from mtDNA to mitochondrial RNA (mtRNA) remains unclear.

104 In vivo, functional defects in mitochondrial RNA (mtRNA) translation and cell respirati

105 Here, we employ CRISPR screening on mitochondrial RNA (mtRNA)-binding proteins and identify

106 nd their genomic association, we report that mitochondrial RNAs (mtRNAs) are attached to the nuclear

107 cerbates DNA toxicity and host death without mitochondrial RNA or DNA depletion; moreover, autophagy

108 kdown of two target genes, alpha-tubulin and mitochondrial RNA polymerase (mtpol), were significantly

109 e promoter DNA template, and mutation of the mitochondrial RNA polymerase (mtRNAP) affect the kinetic

110 embly of an initiation complex that includes mitochondrial RNA polymerase (mtRNAP) and the initiation

111 l promoters requires the concerted action of mitochondrial RNA polymerase (mtRNAP) and transcription

112 Despite identification of the mitochondrial RNA polymerase (mtRNAP) from several organ

113 ication and functional characterization of a mitochondrial RNA polymerase (mtRNAP) from Trypanosoma b

114 Mitochondrial RNA polymerase (mtRNAP) is crucial in cell

115 The mitochondrial RNA polymerase (mtRNAP) of Saccharomyces c

116 chondrial DNA are transcribed by a dedicated mitochondrial RNA polymerase (mtRNAP) that is encoded in

117 tro transcription system with purified human mitochondrial RNA polymerase (POLRMT) and transcription

118 drial DNA (mtDNA) into nucleoids and recruit mitochondrial RNA polymerase (POLRMT) at specific promot

119 We show that the PPR domain of Drosophila mitochondrial RNA polymerase (PolrMT) has 3'-to-5' exori

120 shown that human MRPL12 binds and activates mitochondrial RNA polymerase (POLRMT), and hence has dis

121 omplex comprising mitochondrial transcripts, mitochondrial RNA polymerase (POLRMT), pentatricopeptide

122 hain termination when misincorporated by the mitochondrial RNA polymerase (POLRMT).

123 The yeast mitochondrial RNA polymerase (RNAP) is a two-subunit enz

124 The yeast mitochondrial RNA polymerase (RNAP) is composed of the c

125 n, we show that the Saccharomyces cerevisiae mitochondrial RNA polymerase (Rpo41) and its transcripti

126 ates, we have discovered that the yeast core mitochondrial RNA polymerase (Rpo41) has the intrinsic a

127 22 bp protein-coding region of the T. brucei mitochondrial RNA polymerase (TBMTRNAP) gene is predicte

128 uence similarity to Saccharomyces cerevisiae mitochondrial RNA polymerase and bacteriophage RNA polym

129 mtDNA promoters, LSP and HSP1, only requires mitochondrial RNA polymerase and h-mtTFB2 in vitro.

130 hybrid lines with different combinations of mitochondrial RNA polymerase and mtDNA genotypes.

131 an transcription elongation factor TEFM with mitochondrial RNA polymerase and nascent transcript prev

132 monstrated that mtDNA transcription requires mitochondrial RNA polymerase and Tfam, a DNA binding sti

133 ext of editing sites and the accuracy of the mitochondrial RNA polymerase argue that the mechanism of

134 that occur between the subunits of the yeast mitochondrial RNA polymerase can serve as a simple model

135 p shows a clear preference for ATP and human mitochondrial RNA polymerase does not show significant i

136 cations of TFAM increase the processivity of mitochondrial RNA polymerase during transcription throug

137 Binding of Mss116p stabilizes paused mitochondrial RNA polymerase elongation complexes in vit

138 modulates the activity of the single-subunit mitochondrial RNA polymerase encoded by RPO41.

139 ressed from an alternative transcript of the mitochondrial RNA polymerase gene (POLRMT).

140 Lines bearing certain mtDNA-mitochondrial RNA polymerase genotypic combinations show

141 nal domain (ATD) of Saccharomyces cerevisiae mitochondrial RNA polymerase has been shown to provide a

142 CSB specifically promoted elongation by the mitochondrial RNA polymerase in vitro.

143 The amino-terminal domain (ATD) of yeast mitochondrial RNA polymerase is required to couple trans

144 Mitochondrial RNA polymerase is thought to be the primas

145 ndrial role by directly interacting with the mitochondrial RNA polymerase POLRMT to stimulate mtDNA t

146 issues present dsRNA foci, and inhibition of mitochondrial RNA polymerase reduces systemic inflammati

147 fficiency of transcription initiation by the mitochondrial RNA polymerase Rpo41 and its initiation fa

148 dies of the yeast (Saccharomyces cerevisiae) mitochondrial RNA polymerase Rpo41 and its transcription

149 ient petite phenotype of a point mutation in mitochondrial RNA polymerase that affects mitochondrial

150 es, we demonstrate LRP130 complexes with the mitochondrial RNA polymerase to activate mitochondrial t

151 racterization of a developmentally regulated mitochondrial RNA polymerase transcript in the parasitic

152 inhibition of human polymerases and cellular mitochondrial RNA polymerase up to 100 muM.

153 n initiation factors TFAM and TFB2M on human mitochondrial RNA polymerase, and interactions of the la

154 ranscription machinery (NEP), related to the mitochondrial RNA polymerase, has been recognized only r

155 NA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in ma

156 P-IV lacks 262 amino-terminal amino acids of mitochondrial RNA polymerase, including the mitochondria

157 Human mitochondrial RNA polymerase, POLRMT, is required for mi

158 esponsible for enhancing the processivity of mitochondrial RNA polymerase, POLRMT.

159 length heterogeneity on the activity of the mitochondrial RNA polymerase, POLRMT.

160 factors, TFAM and TFB2M, in addition to the mitochondrial RNA polymerase, POLRMT.

161 hat during in vitro transcription with human mitochondrial RNA polymerase, stable and persistent RNA-

162 Inhibition of the mitochondrial RNA polymerase, the dsRNA sensors RIGI and

163 stem comprising the bacteriophage T7-related mitochondrial RNA polymerase, the rRNA methyltransferase

164 f the mitochondrial transcription apparatus (mitochondrial RNA polymerase, transcription factor 2B an

165 involves transient pausing by the Physarum: mitochondrial RNA polymerase.

166 ingle-subunit RNA polymerases, including the mitochondrial RNA polymerase.

167 are products of transcription synthesized by mitochondrial RNA polymerase.

168 inhibition of human polymerases and cellular mitochondrial RNA polymerase.

169 the presence of newly synthesized RNA and/or mitochondrial RNA polymerase.

170 tive nucleoids and a direct interaction with mitochondrial RNA polymerase.

171 poisomerase I was co-immunoprecipitated with mitochondrial RNA polymerase.

172 or recruitment of h-mtTFB1, h-mtTFB2 and the mitochondrial RNA polymerase.

173 Mitochondrial RNA polymerases (MtRNAPs) are members of t

174 Mitochondrial RNA polymerases depend on initiation facto

175 n of which exhibits 29-37% identity with the mitochondrial RNA polymerases from other organisms in th

176 serve as transcription initiation factors of mitochondrial RNA polymerases in Saccharomyces cerevisia

177 concentrations of exogenous nucleotides the mitochondrial RNA polymerases stall, generating a popula

178 ne, identified by sequence conservation with mitochondrial RNA polymerases, encodes the NEP catalytic

179 n the context of transcription initiation by mitochondrial RNA polymerases.

180 analyze the stability of different T. brucei mitochondrial RNA populations.

181 difications after release from polycistronic mitochondrial RNA precursors, which is essential for mit

182 te for endoribonucleolytic cleavage by RNase mitochondrial RNA processing (MRP) and mutations in the

183 RNase mitochondrial RNA processing (MRP) is a ribonucleoprotei

184 In the yeast Saccharomyces cerevisiae, RNase mitochondrial RNA processing (MRP) is an essential endor

185 RNase mitochondrial RNA processing (MRP) is an essential ribon

186 RNase mitochondrial RNA processing (MRP) is an essential, evol

187 igh-resolution structures of yeast RNase for mitochondrial RNA processing (MRP), a catalytic ribonucl

188 as potential substrates for mammalian RNase mitochondrial RNA processing (MRP).

189 RNase mitochondrial RNA processing (RNase MRP) mutants have be

190 Several steps of mitochondrial RNA processing and maturation, including R

191 as an RNA demethylase that controls nascent mitochondrial RNA processing and mitochondrial activity.

192 PNPASE reduction impaired mitochondrial RNA processing and polycistronic transcrip

193 that splice-variants of proteins involved in mitochondrial RNA processing and translation may be invo

194 nd characterize the structural components of mitochondrial RNA processing and translation, the Mammal

195 he long noncoding RNA RNase component of the mitochondrial RNA processing endoribonuclease (RMRP) giv

196 er insight into the role of RNA Component of Mitochondrial RNA Processing Endoribonuclease (RMRP) in

197 crease in the levels of the RNA component of mitochondrial RNA processing endoribonuclease.

198 RNase mitochondrial RNA processing enzyme (MRP) is a nucleolar

199 d in association with both RNase P and RNase mitochondrial RNA processing in immunoprecipitates from

200 n RNA editing, which is an essential part of mitochondrial RNA processing in trypanosomes.

201 ion of human signal recognition particle and mitochondrial RNA processing RNAs isolated from HeLa cel

202 n of ALKBH7 leads to increased polycistronic mitochondrial RNA processing, reduced steady-state mitoc

203 a lncRNA-derived micropeptide that disrupts mitochondrial RNA processing, revealing a new layer of m

204 evisiae gene deletion strains for defects in mitochondrial RNA processing, we found that lack of any

205 e onset disorders, resulting from defects in mitochondrial RNA processing.

206 Here we report that PNC components mitochondrial RNA-processing (MRP) RNA, pyrimidine tract

207 mutated and wild-type mtPAP localized to the mitochondrial RNA-processing granules thereby eliminatin

208 Mapping and quantitation of chimeric mitochondrial RNAs provide an accessible, orthogonal app

209 omplexes, altering mitoribosome assembly and mitochondrial RNA pseudouridylation.

210 eover, loss of ppr results in a reduction in mitochondrial RNAs, reduced electron transport chain act

211 function, likely through a conserved role in mitochondrial RNA regulation.

212 Gln) in maintenance of mitochondrial genome, mitochondrial RNA stability, translation, and respirator

213 ew of them are identified with a function in mitochondrial RNA stability.

214 mall (12S, MT-RNR1) and large (16S, MT-RNR2) mitochondrial RNA subunits of the mitochondrial ribosome

215 organello, we show that hTERT binds various mitochondrial RNAs, suggesting that RT activity in the o

216 rRNA maturation by-products and as part of a mitochondrial RNA surveillance pathway that eliminates s

217 g and import activities were separable and a mitochondrial RNA targeting signal was isolated that ena

218 nto foci that depend on the transcription of mitochondrial RNAs that may form double-stranded RNA (ds

219 psi residues per RNA varied from one in the mitochondrial RNAs to 57 in the cytoplasmic LSU RNA of D

220 mitochondria, here we present evidence that mitochondrial RNA transcripts (mtRNA) are not limited to

221 f d-mtTFB2 reduces the abundance of specific mitochondrial RNA transcripts 2- to 8-fold and decreases

222 of d-mtTFB2 increases both the abundance of mitochondrial RNA transcripts and the copy number of mtD

223 FB1 did not increase either the abundance of mitochondrial RNA transcripts or mitochondrial DNA copy

224 B1 does not change the abundance of specific mitochondrial RNA transcripts, nor does it affect the co

225 duals have reduced levels of promoter distal mitochondrial RNA transcripts.

226 of the two uncharacterized genes also affect mitochondrial RNA turnover.

227 Recently, we developed mitochondrial RNA vectors that can be used to address an

228 osoma cruzi, edit their post-transcriptional mitochondrial RNA via a multiprotein complex called the

229 n of mitochondrial mRNA and transcription of mitochondrial RNA were suppressed, whereas mRNA expressi

230 The 3'-termini of maize mitochondrial RNAs were characterized by ligation of an

231 s typical of early embryos, or ribosomal and mitochondrial RNAs, while a majority of the remainder co

232 volved in generation of the 5'-ends of other mitochondrial RNAs, whose 5'-ends coincide with the 3'-e

233 ecules could be visualized by labeling total mitochondrial RNA with [alpha-32P]GTP and guanylyl trans