ライフサイエンスコーパス: mobile phase

1 nolithic columns and carbon dioxide/methanol mobile phase.

2 C18 column with ACN-formic acid 0.1% as the mobile phase.

3 percritical fluid chromatography using a CO2 mobile phase.

4 nitrile: water (75:25, v/v) is proposed as a mobile phase.

5 red in-line ionic strength adjustment of the mobile phase.

6 tonitrile each containing formic acid as the mobile phase.

7 aqueous methanol was applied as the washing mobile phase.

8 phy (UTLC) plates in 1-2 min using a ternary mobile phase.

9 etonitrile/1% CH3COOH (82/18) as the loading mobile phase.

10 ample treatment other than dilution with the mobile phase.

11 ium acetate water solution and methanol as a mobile phase.

12 dium (-)-dibenzoyl-l-tartarate as the chiral mobile phase.

13 e column to rid the ion-pair reagents of the mobile phase.

14 nd phosphate buffered saline solution as the mobile phase.

15 ationary phase and bile salt solution as the mobile phase.

16 series using a gradient of hexane-2-propanol mobile phase.

17 sensor and a PDZ domain (MAGI-1 PDZ1) in the mobile phase.

18 5 min using NH(4)NO(3) solution at pH 8.8 as mobile phase.

19 ttributed to the presence of methanol in the mobile phase.

20 d chromatography using an anionic surfactant mobile phase.

21 nts directly into the analyte solution or LC mobile phase.

22 y changing the amount of acetonitrile in the mobile phase.

23 t originally present in the sample or in the mobile phase.

24 e LC sample injection and delivery of the LC mobile phase.

25 and concentration of organic modifier in the mobile phase.

26 rometry (LC-MS) with methanol as the organic mobile phase.

27 n of hydrophobic albumin in a highly aqueous mobile phase.

28 ry phase and methanol: water (98:02, v/v) as mobile phase.

29 data were acquired using acetonitrile as the mobile phase.

30 -bungarotoxin (alpha(x)beta(y) nAChR) to the mobile phase.

31 ups, and the ability to use a highly aqueous mobile phase.

32 onitrile in water with 0.1% formic acid as a mobile phase.

33 ation/immobilization (s/i) buffer and to the mobile phase.

34 eluted with a gradient acetate-acetonitrile mobile phase.

35 -polar compounds employing a totally organic mobile phase.

36 components using an aqueous, non-denaturing mobile phase.

37 h 2% acetic acid/methanol (96:4, v/v) as the mobile phase.

38 cetonitrile/0.1% trifluoroacetic acid as the mobile phase.

39 dryness and the residue was reconstituted in mobile phase.

40 le (ACN): 0.1% phosphoric acid (60:10:30) as mobile phase.

41 of mixed-solvent liquid chromatography (LC) mobile phases.

42 ating the separation temperature under basic mobile phases.

43 egrees C and zero magnesium concentration in mobile phases.

44 lectivity, using both organic and water-rich mobile phases.

45 en added (generally, 0.1%), were employed as mobile phases.

46 rly observed for peptides when using high-pH mobile phases.

47 retention of solutes with high water-content mobile phases.

48 aqueous ammonium formate and acetonitrile as mobile phases.

49 lammable solvents (e.g., hexane) are used as mobile phases.

50 e positive mode of ionization with common LC mobile phases.

51 on of liquefied CO(2) to conventional liquid mobile phases.

52 ary phases and step-gradient aqueous-ethanol mobile phases.

53 s to separate intact proteins using volatile mobile phases.

54 ted with a series of buffered methanol-water mobile phases (20/80, v/v).

55 This novel technique employs a mobile phase 50 mM ammonium acetate for high sensitivity

56 n chromatography (SEC) with an MS-compatible mobile phase (59% water, 40% isopropanol, 1% formic acid

57 C) are demonstrated with typical low density mobile phases (94% CO2).

58 Mobile phase A was 20mmolL(-1) ethylenediaminetetraaceti

59 anol, 14.5mM triethylamine, and 5% methanol (mobile phase A) and 385mM hexafluoro-2-propanol, 14.5mM

60 tion into plasma allowed use of organic-rich mobile phase, achieving species separation in 4 min.

61 city of the sample matrix and sensitivity to mobile phase acidity, are identified and resolved.

62 by using the alternative means of expressing mobile phase acidity.

63 rifluoroacetic acid (TFA) is a commonly used mobile phase additive in liquid chromatography-mass spec

64 In this study, the effects of volatile mobile phase additives on SEC retention and ESI of prote

65 In this study, different mobile phase additives were tested in order to improve t

66 t the need for ion-pairing reagents or other mobile phase additives.

67 und to contain cluster ions corresponding to mobile phase additives.

68 ce of polar mobile-phase solutions or acidic mobile-phase additives (e.g., formic or trifluoroacetic

69 ILIC/MS method was optimized on the basis of mobile-phase additives and pH, followed by evaluation of

70 The mobile phase affects the ionization state of analytes an

71 Ammonium fluoride, added to the mobile phase, aids in the formation of pseudomolecular o

72 ss distribution of the compound between the "mobile phase" (air and dust particles settled on the car

73 t variations in analyte diffusivities in the mobile phase, analyte elution temperatures, optimal line

74 hyl acetate/formic acid (6:10:1, v/v) as the mobile phase and 1% vanillin hydrochloric solution as st

75 size exclusion chromatography (SEC) such as mobile phase and column interaction effects on protein s

76 arise due to the compressible nature of the mobile phase and detector idiosyncrasies to eliminate ba

77 selected proteins were eluted with an acidic mobile phase and identified in two ways.

78 M, determination rate, ca. 10 samplesh(-1), mobile phase and ISA solution consumption, ca. 2.6 mL pe

79 d 1.65 mL/min, with pure acetonitrile as the mobile phase and naphtho[2,3-a]pyrene as the retained co

80 radient elution in acidulated water-methanol mobile phase and octadecyl-silica columns.

81 pH units, depending on the initial pH of the mobile phase and the applied current.

82 The gradient composition of the mobile phase and the flow rate were optimized with respe

83 rstanding of the processes that occur in the mobile phase and the stationary phase.

84 compounds in a variety of acetonitrile/water mobile phases and at different temperatures.

85 has been made transferable between different mobile phases and instrument setups by using a suitable

86 the use of solvents other than those of the mobile phase, and (iii) the need to stop the mobile phas

87 Ammonium nitrate, as the mobile phase, and a suitable rinsing regime, allowed goo

88 the missing cofactor is added to the column mobile phase, and the enzyme converts substrate into pro

89 ffect of matrices, concentrations of aqueous mobile phase, and types of LC modifiers.

90 heir increasing of retention at organic-rich mobile phases (approximately >90% v/v for acetonitrile w

91 neither for the hydrodynamic pumping of the mobile phase as in high-performance liquid chromatograph

92 hy using methanesulfonic acid (75 mM) as the mobile phase at 0.25 mL/min flow rate.

93 nol, 14.5mM triethylamine, and 90% methanol (mobile phase B).

94 (NH4)2CO3 in 1% v/v methanol (pH 9.0) formed mobile phase B.

95 that nonspherical particles can both reduce mobile-phase band broadening and increase permeability c

96 tion gradient with acetonitrile and water as mobile phases (both with formic acid at 0.1%).

97 particle: acetonitrile in the interparticle mobile phase, C(18)-chain associated acetonitrile, and a

98 aining peptide eluted out of LC in an acidic mobile phase can be rapidly reduced prior to MS analysis

99 on behavior of bases (cationic acids) in the mobile phases can be better predicted by using the pH(ab

100 articles settled on the carpet) and the "non-mobile phase" (carpet fibers and pad) and the removal ra

101 peration to retain detector sensitivity when mobile phase changes.

102 Of the 132 column and mobile phase combinations examined for each mixture, a s

103 column temperature, and different column and mobile phase combinations).

104 th peak distortions due to strongly retained mobile phase components in other modes of liquid chromat

105 Mobile phase components needed to be sufficiently volati

106 The influence of mobile-phase components on chromatographic performance a

107 The use of a mobile phase composed by ammonium formate-methanol in a

108 amines were separated on C18 column using a mobile phase composed of ammonium formate buffer and ace

109 lysis employed silica gel-coated TLC plates, mobile phase composed of chloroform:methanol:water:25% a

110 the elution of compounds in a higher organic mobile phase composition (retention times were approxima

111 degrees C), flow rate (1.0-2.5 mL min(-1)), mobile phase composition and equilibration time (1-5 min

112 ssed as are the effects of mat thickness and mobile phase composition on the chromatographic properti

113 cts, e.g. flow rate, column temperature, and mobile phase composition on the response variable.

114 Non-chlorinated mobile phase composition was optimized for separation of

115 The mobile phase composition was optimized to maintain the p

116 < linear < ccc, is constant upon changes of mobile phase composition, gradient slope, and plasmid si

117 ntion factors at extremes of temperature and mobile phase composition.

118 ic conditions includes the column selection, mobile-phase composition, pH value, buffer type, and con

119 aration was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) an

120 was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on

121 eversed phase HPLC-ICP-MS, such as pH of the mobile phase, concentration of ion pairing reagents, typ

122 Both isocratic and gradient mobile phase conditions were used.

123 aphic columns when used under highly aqueous mobile-phase conditions.

124 n Hibar C18 column (250x4.6mm, 5micro) using mobile phase consist of acetonitrile: water (90:10, v/v)

125 The mobile phase consisted of 0.2 M phosphate buffer (pH 7.0

126 tion on silica gel-coated HPTLC plates using mobile phase consisted of chloroform:methanol:acetone:25

127 The mobile phase consisted of water and acetonitrile, with a

128 -crystalline bilayer phase, and an extremely mobile phase consistent with small vesicles or micelles.

129 c separation was achieved on C8 column using mobile phase consisting of (A) methanol:acetonitrile (8:

130 mm, 5 mum) column by gradient elution with a mobile phase consisting of 0.1% trifluoroacetic acid (pH

131 ne in food supplements was performed using a mobile phase consisting of 100% methanol, column tempera

132 ned on a Chiralcel(R) OJ-RH column using the mobile phase consisting of 10mM aqueous ammonium acetate

133 hic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroace

134 enyl column (50 x 2.0 mm) and supported by a mobile phase consisting of acetonitrile plus 0.1% formic

135 mm, 5 mum) column by gradient elution with a mobile phase consisting of ammonium acetate 0.05 M and a

136 An optimized mobile phase consisting of methanol and 25 mM triethylam

137 achieved using a C18 column with a gradient mobile phase consisting of solvents A (0.1% formic acid

138 thylene bridged hybrid phenyl column using a mobile phase consisting of water and methanol containing

139 The HILIC mobile phase contained 25 mM ammonium acetate and allowe

140 ntrary to previous applications in which the mobile phase contained water, the improvement in sensiti

141 er nondenaturing conditions using a buffered mobile phase containing 5% 2-propanol.

142 The optimized CEC-ESI-MS conditions were mobile phase containing 90/10 ACN/2.5 mM Tris, pH 8, she

143 olar organic mode (e.g., methanol or ethanol mobile phase containing a barium salt additive).

144 alyzed by reversed-phase HPLC method using a mobile phase containing acetonitrile:methanol:2-propanol

145 bolites was carried out isocratically with a mobile phase containing both positively and negatively c

146 The mobile phase containing CLA isomers eluting from the Ag(

147 ed-phase liquid chromatography (IPRP) with a mobile phase containing triethylamine (TEA) and hexafluo

148 lumn, acetonitrile-trifluoroacetic acid as a mobile phase, coupled with UV detector at 205 nm, was su

149 tes and matrix constituents solubility after mobile phase decompression.

150 n the retention factor (K) and the pH of the mobile phase demonstrate that the binding of cis-diols t

151 the dependency of solute retention with the mobile-phase density, complicating linear extrapolation

152 ution with mixtures of methanol and water as mobile phases; detection wavelength was set at 240 nm fo

153 his metric appears to be a good indicator of mobile-phase dispersion in ordered packed bed media, inc

154 ction of the mass transfer resistance in the mobile phase due to a flatter flow profile and faster an

155 tered when moving from an aqueous to organic mobile phase during a gradient elution, a key analytical

156 mobile phase, and (iii) the need to stop the mobile phase flow at any time during the full analytical

157 meters such as column type, buffer solution, mobile phase flow rate and sample injection volume were

158 0.1% formic acid (50:50, v/v) and an optimal mobile phase flow rate of 0.2 mL/min.

159 NPs exhibited a retention time of 771 s at a mobile phase flow rate of 1 mL min(-1).

160 h direct injection onto the PLOT column at a mobile phase flow rate of 20 nL/min.

161 ides excellent chromatographic resolution at mobile phase flow rates from 1 to 55 muL min(-1).

162 ns for the generation of Fe nanoparticles, a mobile phase for As adsorption currently not a part of r

163 An anionic micellar medium was added to the mobile phase for increasing the fluorescence intensity a

164 methanol/aqueous trifluoroacetic acid as the mobile phase for size exclusion chromatography-ESI-MS an

165 spectrometry detection sensitivity than TFA mobile phases for LC-MS-based characterization of biopha

166 se low vapor pressure ionic liquids as their mobile phases for sensing atmospheric analytes.

167 nt column temperature, stationary phase, and mobile phase gradient conditions.

168 iagrams as a function of column temperature, mobile phase gradient or a multifactorial combination in

169 imized for organic modifier, ionic modifier, mobile phase gradient, flow rate, column type, MS source

170 ction with chromatographic separations using mobile phase gradients.

171 reagent, m-nitrobenzyl alcohol (m-NBA), into mobile phases greatly facilitates the analysis of acidic

172 purpose, the addition of thiosulfate to the mobile phase has been used to elute Ag(I) species from t

173 ) values for a number of common LC and LC-MS mobile phases have been determined.

174 in the DLLME with the reduced consumption of mobile phase in capillary HPLC.

175 out using water and ammonium bicarbonate as mobile phase in gradient mode.

176 ition of these supercharging reagents to the mobile phase in liquid chromatography (LC)-MS/MS increas

177 e change in the refractive index (RI) of the mobile phase in very fast gradients causes extremely ser

178 imation of the volumes of the stationary and mobile phases in dynamic equilibrium with eluents of var

179 The key feature is a mobile phase, in which hydrophilic and hydrophobic compo

180 At organic-rich mobile phases, in fact, stationary phases are characteri

181 ither post-column or by incorporation in the mobile phase increases specificity for all of the MC whi

182 ion following an increase of acetonitrile in mobile phase) initially exhibited by perfluoroalkyl acid

183 tation pattern, without any matrix effect or mobile-phase interference.

184 ndent accumulation of pyrene from an aqueous mobile phase into the center of individual C18-chromatog

185 d from potential contamination); (IV) the LC mobile phase is of much lower viscosity with respect to

186 y obtained with ammonium tartrate in the HIC mobile phases is orthogonal to that of reverse phase chr

187 stability of RNA in the denaturing/basic IEX mobile phase, lay out a protocol to determine the on-the

188 luate and correct for the impact of stagnant mobile phase mass transfer.

189 dge RP-18e (Merck), 10x4.6mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rat

190 The aqueous mobile phase migrates only through the channel due to th

191 ts in SFC separations using a nontraditional mobile phase mixture consisting of ammonium hydroxide co

192 Further, they were used in all mobile phase modes and with high flow rates and pressure

193 polarities amenable to pSFC with appropriate mobile-phase modifiers and additives under normal-phase

194 phenyl-2-oxoethanal as the negative-ion-mode mobile-phase modifiers for the analysis of peptides.

195 alcohol to the water (W)-acetonitrile (ACN) mobile phase (MP).

196 on a Synergi Hydro analytical column with a mobile phase of 0.02 M ammonium formate in water and ace

197 e quinolones were resolved in <22min using a mobile phase of 0.05M SDS - 7.5% 1-propanol - 0.5% triet

198 ones were eluted without interferences using mobile phase of 0.05M SDS/12.5% 1-propanol/0.5% triethyl

199 Using a mobile phase of 0.2 M ammonium acetate (pH 6.9), several

200 uorescence detection within 10min by using a mobile phase of acetonitrile and acetate buffer of pH 6.

201 ected to high-performance LC with an optimal mobile phase of acetonitrile-water containing 0.1% formi

202 Isocratic elution was used with a mobile phase of ACN: aqueous ammonium formate 1 mM with

203 used under the optimum conditions predicted: mobile phase of H2SO4 0.005 mol L(-1) solution, flow rat

204 Upon addition of 0.2% TFE to the mobile phase of nLC/MS experiments, tryptic peptide iden

205 Using a mobile phase of water:acetonitrile:methanol (83:6:11) at

206 th a C18 column (2.1 x 100 mm, 1.7 mum) with mobile phases of 0.1% formic acid in water and acetonitr

207 different stationary phases in contact with mobile phases of various water/methanol ratios.

208 and the final methanol concentration in the mobile phase on the peak resolution and peak symmetry wa

209 and ensured few changes were required in the mobile phase or other parameters.

210 Different mobile phase organic solvents and ion-pairing reagents w

211 ditions and subsequent stability analysis in mobile phase, PBS buffer, and rat serum of 12 aryl sulfo

212 mobile phase velocities, the friction of the mobile phase percolating through the column bed generate

213 of intact protein mixtures using a different mobile phase pH in each dimension.

214 Mobile phase pH is very important in LC, but its correct

215 reagent (IPR) concentration, counterion, and mobile phase pH on the quality of the RPIP-UPLC separati

216 We have found that a mobile phase pH value near the pI of the zwitterionic ad

217 c nature of proteins, the use of a different mobile phase pH was successful to provide altered select

218 on (<9.9%CV), recovery (83-99%), robustness (mobile phase pH, column temperature and flow rate) and s

219 hromatographic parameters (stationary phase, mobile phase pH, temperature, organic solvent, and gradi

220 s used to relate analyte retention time with mobile-phase pH and organic modifier content.

221 cetonitrile content, salt concentration, and mobile-phase pH with R(2) > 0.95.

222 rious modes of protein chromatography at any mobile-phase pH, which may have significant implications

223 combinations of organic modifier content and mobile-phase pH.

224 phase column with gradient elution of basic mobile phases (pH 9.2).

225 r peak shapes, particularly when eluted with mobile phases preferred for electrospray ionization mass

226 er, the water/acetonitrile and water/acetone mobile phases produced the better chromatographic separa

227 In this way, mobile phase protons are prevented from interfering with

228 The addition of liquefied CO(2) to the mobile phase provided an electrospray ionization (ESI)-f

229 he discovery of this glycine additive in TFA mobile phases provides a simple and conventional approac

230 ncrease of organic modifier, over the entire mobile phase range.

231 bicarbonate and pyridine buffer solutions as mobile phases, respectively.

232 ively high percentage of acetonitrile in the mobile phase, resulting in stable and high efficiency io

233 ng the retention of cationic analytes as the mobile-phase salt content is varied.

234 of amphiphilic (surfactant) molecules to the mobile-phase solution in order to bring about the retent

235 nary phase particles with acetonitrile-water mobile phase solutions by confocal Raman microscopy.

236 bilization, because of the presence of polar mobile-phase solutions or acidic mobile-phase additives

237 Herein, we optimize the choice of mobile phase solvent in a gradient program with three pa

238 alcohols on the particle surface and in the mobile phase solvent.

239 With the use of water-glycerol mobile phase spanning a wide range of viscosities, the o

240 Other small molecule additives in TFA mobile phases, such as amino acids containing extended s

241 rabutylammonium for carboxylic acids) in the mobile phase suppresses the ESI-MS signals in the gas ph

242 uted using a gradient methanol/water/toluene mobile phase system at a flow rate of 0.5 mL min(-1).

243 The gradient mobile phase system consisted of 385mM hexafluoro-2-prop

244 The overall performance of this mobile phase system was found comparable to ammonium ace

245 CX mixing ratio as the ionic strength of the mobile phase system.

246 ationary phases in conjunction with multiple mobile-phase systems, as applied to the separation of 45

247 ous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a

248 The column, mobile phase, temperature and flow rate were optimised t

249 e successive physical transformations of the mobile phase that take place in very high pressure liqui

250 in their ability to resolve glycoforms using mobile phases that are compatible with online liquid chr

251 ntial-oil "matrix", replacing it with the LC mobile phase (the GC system is more protected from poten

252 Because water was the mobile phase, the retention factor could not be kept con

253 Indeed it was found that, at organic-rich mobile phases, the transfer from the mobile to the stati

254 olumn is eluted with a predominantly organic mobile phase, then solutes can be retained through hydro

255 such as postcolumn organic enrichment of the mobile phase to enhance ESI efficiency.

256 cellar conditions using 1-2% (v/v) 1-butanol mobile phase to remove plasma proteins and concentrate t

257 r for the transfer of polar species from the mobile phase to residual silanols, and this reduced barr

258 nd to develop gel porosity in contact with a mobile phase ultimately affecting the chromatographic pe

259 aqueous acetic acid/methanol mixture as the mobile phase under gradient conditions.

260 Moreover, the mobile phase used allows high quality on-line MS detecti

261 e linear velocity and the composition of the mobile phase used in the second dimension, its initial o

262 te in 22%v/v methanol solution (pH 4) as the mobile phase using isocratic elution.

263 xtures of small molecules are carried out at mobile phase velocities close to (for isocratic runs) or

264 ith very fine particles are operated at high mobile phase velocities, the friction of the mobile phas

265 axes are linear: column length vertical and mobile phase velocity horizontal.

266 ate height with an increase of around 15% in mobile phase velocity in nonretained measurements of Cou

267 al plates as a function of column length and mobile phase velocity is a surface (z direction) to the

268 HETP tend to increase faster with increasing mobile phase velocity than the calculated values.

269 d due to the influence of temperature on the mobile phase viscosity and on the equilibrium constant o

270 column, the temperature dependencies of the mobile phase viscosity, and the retention factor of the

271 urea, did provide an accurate measure of the mobile-phase volume but only over a limited range of elu

272 a viable measure of the kinetic void volume (mobile-phase volume) of the column.

273 At 2 min, mobile phase was changed to elute and separate PET radio

274 neous heat transfer from the assembly to the mobile phase was obtained.

275 The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82+/-3% and

276 mns were compared and the composition of the mobile phase was optimized to achieve baseline separatio

277 After the mobile phase was switched to a stronger one, these compo

278 obtained with a gradient method in which the mobile phase was water (0.1% acetic acid) as solvent A a

279 ality grades, postcolumn modification of the mobile phase) was investigated in a pragmatic and decisi

280 reas the other set received tap water as the mobile phase water.

281 tion, where the chemical constituents of the mobile phase were modified stepwise during analysis, thi

282 The aqueous and organic mobile phases were 0.1% formic acid in water and acetoni

283 The most superior mobile phases were also applied on instant thin-layer ch

284 For radio-TLC quality control, various mobile phases were analyzed using silica gel 60 plates a

285 Several liquid chromatography stationary and mobile phases were evaluated, and it was found that hydr

286 The (W)(S)pHs of the mobile phases were successively adjusted with addition o

287 h of analytical column, and flow rate of the mobile phase, were optimised for five selenium species;

288 One phase is used as the mobile phase when the other, the stationary phase, is he

289 aration of organic solutes in purely aqueous mobile phases whereby retention is controlled through te

290 ies by anion exchange chromatography using a mobile phase which is a 1:10 dilution of the extracting

291 covered glycine as a simple additive for TFA mobile phases, which mitigates ion suppression through a

292 LC-based column method using a fully aqueous mobile phase with 5 mM CaCl(2) at pH 4.5.

293 h MS detection (HIC-MS) utilizing an aqueous mobile phase with a low concentration of a volatile salt

294 general, androgens required a stronger UPLC mobile phase with a slower flow rate and ESI of the oppo

295 plied using 0.1% formic acid (pH 3.4) as the mobile phase with detection at 260 nm.

296 phobic interaction chromatography (HIC) uses mobile phases with high salt concentration that are not

297 rol was performed on silica gel 60 plates, 4 mobile phases with suitable separation properties and co

298 e-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined

299 ime direct comparison of acidities of any LC mobile phases, with different organic additives, differe

300 adding a small amount (e.g., 2 mM) into TFA mobile phases without compromising the chromatographic p